TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Balog, Zsófia Csuzd
AU  - Dukai, Eva
AU  - Vekić, Jelena
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3802
AB  - Introduction: Indirect estimation of reference intervals (RIs) is straightforward and inexpensive procedure for determination of intra-laboratory RIs. We applied the indirect approach to assess RIs for haematological parameters in capillary blood of pre-school children, using results stored in our laboratory database. Materials and methods: We extracted data from laboratory information system, for the results obtained by automatic haematology analyser in capillary blood of 154 boys and 146 girls during pre-school medical examination. Data distribution was tested, and logarithmic transformation was applied if needed. Reference intervals were calculated by the nonparametric percentile method. Results: Reference intervals were calculated for: RBC count (4.2-5.4 x1012/L), haemoglobin (114-146 g/L), MCH (25.0-29.4 pg), MCHC (321-368 g/L), RDW-SD (36.1-43.5 fL), WBC count (4.5-12.3 x109/L), neutrophils count (1.7-6.9 x109/L) and percentage (29.0-69.0%), lymphocytes count (1.6-4.4 x109/L) and percentage (21.9-60.7%), PLT (165-459 x109/L), MPV (8.1-11.4 fL) and PDW (9.2-14.4%). Gender specific RIs were calculated for mo-nocytes count (male (M): 0.2-1.6 x109/L; female (F): 0.1-1.4 x109/L) and percentage (M: 2.5-18.3%; F: 1.8-16.7%), haematocrit (M: 0.34-0.42 L/L; F: 0.34-0.43 L/L), MCV (M: 73.4-84.6 fL; F: 75.5-84.2 fL) and RDW (M: 12.1-14.3%; F: 11.7-13.9%), due to observed gender differences in these parameters (P = 0.031, 0.028, 0.020, 0.012 and 0.001; respectively). Estimated RIs markedly varied from the literature based RIs that are used in the labora-tory. Conclusions: Indirect method employed in this study enables straightforward assessment of RIs in pre-school children. Herein derived RIs differed from the literature-based ones, indicating the need for intra-laboratory determination of RIs for specific populations and sample types.
PB  - Zagreb : Hrvatsko drustvo medicinskih biokemičara
T2  - Biochemia Medica
T1  - Indirect reference intervals for haematological parameters in capillary blood of pre-school children
VL  - 31
IS  - 1
SP  - 1
EP  - 9
DO  - 10.11613/BM.2021.010709
ER  - 

@article{
author = "Zeljković, Aleksandra and Balog, Zsófia Csuzd and Dukai, Eva and Vekić, Jelena and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna",
year = "2021",
abstract = "Introduction: Indirect estimation of reference intervals (RIs) is straightforward and inexpensive procedure for determination of intra-laboratory RIs. We applied the indirect approach to assess RIs for haematological parameters in capillary blood of pre-school children, using results stored in our laboratory database. Materials and methods: We extracted data from laboratory information system, for the results obtained by automatic haematology analyser in capillary blood of 154 boys and 146 girls during pre-school medical examination. Data distribution was tested, and logarithmic transformation was applied if needed. Reference intervals were calculated by the nonparametric percentile method. Results: Reference intervals were calculated for: RBC count (4.2-5.4 x1012/L), haemoglobin (114-146 g/L), MCH (25.0-29.4 pg), MCHC (321-368 g/L), RDW-SD (36.1-43.5 fL), WBC count (4.5-12.3 x109/L), neutrophils count (1.7-6.9 x109/L) and percentage (29.0-69.0%), lymphocytes count (1.6-4.4 x109/L) and percentage (21.9-60.7%), PLT (165-459 x109/L), MPV (8.1-11.4 fL) and PDW (9.2-14.4%). Gender specific RIs were calculated for mo-nocytes count (male (M): 0.2-1.6 x109/L; female (F): 0.1-1.4 x109/L) and percentage (M: 2.5-18.3%; F: 1.8-16.7%), haematocrit (M: 0.34-0.42 L/L; F: 0.34-0.43 L/L), MCV (M: 73.4-84.6 fL; F: 75.5-84.2 fL) and RDW (M: 12.1-14.3%; F: 11.7-13.9%), due to observed gender differences in these parameters (P = 0.031, 0.028, 0.020, 0.012 and 0.001; respectively). Estimated RIs markedly varied from the literature based RIs that are used in the labora-tory. Conclusions: Indirect method employed in this study enables straightforward assessment of RIs in pre-school children. Herein derived RIs differed from the literature-based ones, indicating the need for intra-laboratory determination of RIs for specific populations and sample types.",
publisher = "Zagreb : Hrvatsko drustvo medicinskih biokemičara",
journal = "Biochemia Medica",
title = "Indirect reference intervals for haematological parameters in capillary blood of pre-school children",
volume = "31",
number = "1",
pages = "1-9",
doi = "10.11613/BM.2021.010709"
}

Zeljković, A., Balog, Z. C., Dukai, E., Vekić, J., Jelić-Ivanović, Z.,& Spasojević-Kalimanovska, V.. (2021). Indirect reference intervals for haematological parameters in capillary blood of pre-school children. in Biochemia Medica
Zagreb : Hrvatsko drustvo medicinskih biokemičara., 31(1), 1-9.
https://doi.org/10.11613/BM.2021.010709

Zeljković A, Balog ZC, Dukai E, Vekić J, Jelić-Ivanović Z, Spasojević-Kalimanovska V. Indirect reference intervals for haematological parameters in capillary blood of pre-school children. in Biochemia Medica. 2021;31(1):1-9.
doi:10.11613/BM.2021.010709 .

Zeljković, Aleksandra, Balog, Zsófia Csuzd, Dukai, Eva, Vekić, Jelena, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, "Indirect reference intervals for haematological parameters in capillary blood of pre-school children" in Biochemia Medica, 31, no. 1 (2021):1-9,
https://doi.org/10.11613/BM.2021.010709 . .