TY  - JOUR
AU  - Toljić, Mina
AU  - Nikolić, Nađa
AU  - Joksić, Ivana
AU  - Čarkić, Jelena
AU  - Munjas, Jelena
AU  - Karadžov Orlić, Nataša
AU  - Milašin, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5615
AB  - Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.
PB  - Elsevier B.V.
T2  - Journal of Reproductive Immunology
T1  - Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus
VL  - 162
SP  - 104211
DO  - 10.1016/j.jri.2024.104211
ER  - 

@article{
author = "Toljić, Mina and Nikolić, Nađa and Joksić, Ivana and Čarkić, Jelena and Munjas, Jelena and Karadžov Orlić, Nataša and Milašin, Jelena",
year = "2024",
abstract = "Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.",
publisher = "Elsevier B.V.",
journal = "Journal of Reproductive Immunology",
title = "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus",
volume = "162",
pages = "104211",
doi = "10.1016/j.jri.2024.104211"
}

Toljić, M., Nikolić, N., Joksić, I., Čarkić, J., Munjas, J., Karadžov Orlić, N.,& Milašin, J.. (2024). Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology
Elsevier B.V.., 162, 104211.
https://doi.org/10.1016/j.jri.2024.104211

Toljić M, Nikolić N, Joksić I, Čarkić J, Munjas J, Karadžov Orlić N, Milašin J. Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology. 2024;162:104211.
doi:10.1016/j.jri.2024.104211 .

Toljić, Mina, Nikolić, Nađa, Joksić, Ivana, Čarkić, Jelena, Munjas, Jelena, Karadžov Orlić, Nataša, Milašin, Jelena, "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus" in Journal of Reproductive Immunology, 162 (2024):104211,
https://doi.org/10.1016/j.jri.2024.104211 . .

TY  - JOUR
AU  - Munjas, Jelena
AU  - Sopić, Miron
AU  - Stefanović, Aleksandra
AU  - Košir, Rok
AU  - Ninić, Ana
AU  - Joksić, Ivana
AU  - Antonić, Tamara
AU  - Spasojević-Kalimanovska, Vesna
AU  - Prosenc Zmrzljak, Uršula
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3979
AB  - Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential
VL  - 22
IS  - 19
DO  - 10.3390/ijms221910652
ER  - 

@article{
author = "Munjas, Jelena and Sopić, Miron and Stefanović, Aleksandra and Košir, Rok and Ninić, Ana and Joksić, Ivana and Antonić, Tamara and Spasojević-Kalimanovska, Vesna and Prosenc Zmrzljak, Uršula",
year = "2021",
abstract = "Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential",
volume = "22",
number = "19",
doi = "10.3390/ijms221910652"
}

Munjas, J., Sopić, M., Stefanović, A., Košir, R., Ninić, A., Joksić, I., Antonić, T., Spasojević-Kalimanovska, V.,& Prosenc Zmrzljak, U.. (2021). Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential. in International Journal of Molecular Sciences
MDPI., 22(19).
https://doi.org/10.3390/ijms221910652

Munjas J, Sopić M, Stefanović A, Košir R, Ninić A, Joksić I, Antonić T, Spasojević-Kalimanovska V, Prosenc Zmrzljak U. Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential. in International Journal of Molecular Sciences. 2021;22(19).
doi:10.3390/ijms221910652 .

Munjas, Jelena, Sopić, Miron, Stefanović, Aleksandra, Košir, Rok, Ninić, Ana, Joksić, Ivana, Antonić, Tamara, Spasojević-Kalimanovska, Vesna, Prosenc Zmrzljak, Uršula, "Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential" in International Journal of Molecular Sciences, 22, no. 19 (2021),
https://doi.org/10.3390/ijms221910652 . .

TY  - JOUR
AU  - Munjas, Jelena
AU  - Sopić, Miron
AU  - Joksić, Ivana
AU  - Prosenc Zmrzljak, Ursula
AU  - Karadžov-Orlić, Nataša
AU  - Košir, Rok
AU  - Egić, Amira
AU  - Miković, Željko
AU  - Ninić, Ana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3669
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]
VL  - 85
SP  - 57
EP  - 57
DO  - 10.1016/j.clinbiochem.2020.08.011
ER  - 

@article{
author = "Munjas, Jelena and Sopić, Miron and Joksić, Ivana and Prosenc Zmrzljak, Ursula and Karadžov-Orlić, Nataša and Košir, Rok and Egić, Amira and Miković, Željko and Ninić, Ana and Spasojević-Kalimanovska, Vesna",
year = "2020",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]",
volume = "85",
pages = "57-57",
doi = "10.1016/j.clinbiochem.2020.08.011"
}

Munjas, J., Sopić, M., Joksić, I., Prosenc Zmrzljak, U., Karadžov-Orlić, N., Košir, R., Egić, A., Miković, Ž., Ninić, A.,& Spasojević-Kalimanovska, V.. (2020). Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]. in Clinical Biochemistry
Elsevier., 85, 57-57.
https://doi.org/10.1016/j.clinbiochem.2020.08.011

Munjas J, Sopić M, Joksić I, Prosenc Zmrzljak U, Karadžov-Orlić N, Košir R, Egić A, Miković Ž, Ninić A, Spasojević-Kalimanovska V. Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]. in Clinical Biochemistry. 2020;85:57-57.
doi:10.1016/j.clinbiochem.2020.08.011 .

Munjas, Jelena, Sopić, Miron, Joksić, Ivana, Prosenc Zmrzljak, Ursula, Karadžov-Orlić, Nataša, Košir, Rok, Egić, Amira, Miković, Željko, Ninić, Ana, Spasojević-Kalimanovska, Vesna, "Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]" in Clinical Biochemistry, 85 (2020):57-57,
https://doi.org/10.1016/j.clinbiochem.2020.08.011 . .

TY  - JOUR
AU  - Joksić, Ivana
AU  - Miković, Željko
AU  - Filimonović, Dejan
AU  - Munjas, Jelena
AU  - Karadžov-Orlić, Nataša
AU  - Egić, Amira
AU  - Joksić, Gordana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3618
AB  - Background:Recurrent pregnancy loss (RPL) is a heterogeneous condition affecting up to 5% of women of reproductive age. Inherited thrombophilia have been postulated as one  of  the  causes  of  RPL.  Here  we  examined  the  prevalence  of  nine  thrombophilic  gene  polymorphisms  among women  with  history  of  recurrent  miscarriages  and  fertile controls.Methods:The study included 70 women with history of at least  three  early  pregnancy  losses  and  31  fertile  controls with  no  miscarriages.  We  investigated  mutations  in  genes responsible  for  clotting  and  fibrinolysis,  including  factor  V(FV) Leiden, FV H1299R, factor II (FII) G20210A, methyl-ene  tetrahydrofolate  reductase  (MTHFR)  C677T  and A1298C,  factor  XIII  (FXIII)  V34L,  plasminogen  activator inhibitor-1 (PAI-1) 4G/5G and endothelial protein C receptor  (EPCR)  H1  and  H3  haplotypes  using  reverse  polymerase  chain  reaction  ViennaLab  cardiovascular  disease StrippAssays. Results:Our  results  showed  no  significant  increase  inprevalence  of  tested  polymorphisms  in  women  with  RPL. However, relative risk for PRL among women heterozygousfor FXIII V34L was 2.81 times increased (OR 2.81, 95% CI1.15–6.87,  P=0.023).  Haplotype  analysis  showed  that combined  presence  of  high-risk  genotypes  for  FXIII  andPAI-1  significantly  increases  risk  for  RPL  (OR  13.98,  CI95% 1.11–17.46, P=0.044).Conclusions:This  is  the  first  study  in  Serbian  population that investigated prevalence of FVR2, A1298C, FXIII V34Land  EPCR  gene  variants.  Compound  heterozygosity  forFXIII V34L and PAI-1 4G is significant risk factor for recur-rent miscarriage. Our results should be viewed in context of small case-control study, so further large prospective studies are need for confirmation of our findings.
AB  - Uvod: Ponavljani spontani pobačaji (PSP) su etiološki heterogeni i javljaju se kod 5% parova u reproduktivnom period. Jedan od mogućih uzroka PSP su i nasledne trombofilije. U okviru ove studije analizirali smo učestalost devet trombofilnih polimorfizama kod pacijentkinja sa ponavljanim spontanim pobačajima. Metode: Ispitanici su u studiji podeljeni u dve grupe na osnovu anamnestičkih podataka o broju spontanih pobačaja (70 u grupi sa PSP i 31 u kontrolnoj grupi). Ispitivani su sledeći genski polimorfizmi: faktor V Lajden (FVL), FVR2, faktor II (FII) G21210A, metilentetrahidrofolat reduktaza (MTHFR) C677T i A1298C polimorfizmi, inhibitor aktivatora plazminogena 1 (PAI-1) 4G/5G, faktor XIII (FXIII) V34L i endotelni protein C receptor (EPRC) H1, H2 i H3 haplotipovi. Za detekciju navedenih polimorfizama je korišćena metoda multipleks reakcije lančanog umnožavanja i reverzne hibridizacije na ViennaLab stripovima. Rezultati: Dobijeni rezultati nisu pokazali povećanu učestalost ispitivanih polimorfizama u grupi sa PSP. Posmatrajući uticaj pojedinačnih polimorfizama na ishod trudnoće pokazano je da polimorfizam FXIII V34L povećava rizik za ponavljane spontane pobačaje (OR 2,81, 95%CI 1,15-6,87, P=0,023). Analizom haplotipova ustanovljeno je da kombinovano prisustvo V34L i PAI-1 4G varijanti značajno povećava rizik za PSP (OR 13,98, CI 95% 1,11-17,46, P=0,044). Zaključak: Ovo je prva studija koja je ispitivala prevalencu FVR2, A1298C, FXIII V34L and EPCR polimorfizama u populaciji žena iz Srbije. Složeni heterozigoti za FXIII V34L i PAI-1 4G polimorfizme imaju značajno povišen rizik sa ponavljane gubitke trudnoće. Radi potvrde dobijenih rezultata potrebne su veće prospektivne studije.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in serbian population
T1  - Kombinovano prisustvo genskih polimorfizma faktora koagulacije XIII V34L i inhibitora plazminogen aktivatora 1 4G/5G značajno utiče na rizik od spontanog pobačaja u srpskoj populaciji
VL  - 39
IS  - 2
SP  - 199
EP  - 207
DO  - 10.2478/jomb-2019-0028
ER  - 

@article{
author = "Joksić, Ivana and Miković, Željko and Filimonović, Dejan and Munjas, Jelena and Karadžov-Orlić, Nataša and Egić, Amira and Joksić, Gordana",
year = "2020",
abstract = "Background:Recurrent pregnancy loss (RPL) is a heterogeneous condition affecting up to 5% of women of reproductive age. Inherited thrombophilia have been postulated as one  of  the  causes  of  RPL.  Here  we  examined  the  prevalence  of  nine  thrombophilic  gene  polymorphisms  among women  with  history  of  recurrent  miscarriages  and  fertile controls.Methods:The study included 70 women with history of at least  three  early  pregnancy  losses  and  31  fertile  controls with  no  miscarriages.  We  investigated  mutations  in  genes responsible  for  clotting  and  fibrinolysis,  including  factor  V(FV) Leiden, FV H1299R, factor II (FII) G20210A, methyl-ene  tetrahydrofolate  reductase  (MTHFR)  C677T  and A1298C,  factor  XIII  (FXIII)  V34L,  plasminogen  activator inhibitor-1 (PAI-1) 4G/5G and endothelial protein C receptor  (EPCR)  H1  and  H3  haplotypes  using  reverse  polymerase  chain  reaction  ViennaLab  cardiovascular  disease StrippAssays. Results:Our  results  showed  no  significant  increase  inprevalence  of  tested  polymorphisms  in  women  with  RPL. However, relative risk for PRL among women heterozygousfor FXIII V34L was 2.81 times increased (OR 2.81, 95% CI1.15–6.87,  P=0.023).  Haplotype  analysis  showed  that combined  presence  of  high-risk  genotypes  for  FXIII  andPAI-1  significantly  increases  risk  for  RPL  (OR  13.98,  CI95% 1.11–17.46, P=0.044).Conclusions:This  is  the  first  study  in  Serbian  population that investigated prevalence of FVR2, A1298C, FXIII V34Land  EPCR  gene  variants.  Compound  heterozygosity  forFXIII V34L and PAI-1 4G is significant risk factor for recur-rent miscarriage. Our results should be viewed in context of small case-control study, so further large prospective studies are need for confirmation of our findings., Uvod: Ponavljani spontani pobačaji (PSP) su etiološki heterogeni i javljaju se kod 5% parova u reproduktivnom period. Jedan od mogućih uzroka PSP su i nasledne trombofilije. U okviru ove studije analizirali smo učestalost devet trombofilnih polimorfizama kod pacijentkinja sa ponavljanim spontanim pobačajima. Metode: Ispitanici su u studiji podeljeni u dve grupe na osnovu anamnestičkih podataka o broju spontanih pobačaja (70 u grupi sa PSP i 31 u kontrolnoj grupi). Ispitivani su sledeći genski polimorfizmi: faktor V Lajden (FVL), FVR2, faktor II (FII) G21210A, metilentetrahidrofolat reduktaza (MTHFR) C677T i A1298C polimorfizmi, inhibitor aktivatora plazminogena 1 (PAI-1) 4G/5G, faktor XIII (FXIII) V34L i endotelni protein C receptor (EPRC) H1, H2 i H3 haplotipovi. Za detekciju navedenih polimorfizama je korišćena metoda multipleks reakcije lančanog umnožavanja i reverzne hibridizacije na ViennaLab stripovima. Rezultati: Dobijeni rezultati nisu pokazali povećanu učestalost ispitivanih polimorfizama u grupi sa PSP. Posmatrajući uticaj pojedinačnih polimorfizama na ishod trudnoće pokazano je da polimorfizam FXIII V34L povećava rizik za ponavljane spontane pobačaje (OR 2,81, 95%CI 1,15-6,87, P=0,023). Analizom haplotipova ustanovljeno je da kombinovano prisustvo V34L i PAI-1 4G varijanti značajno povećava rizik za PSP (OR 13,98, CI 95% 1,11-17,46, P=0,044). Zaključak: Ovo je prva studija koja je ispitivala prevalencu FVR2, A1298C, FXIII V34L and EPCR polimorfizama u populaciji žena iz Srbije. Složeni heterozigoti za FXIII V34L i PAI-1 4G polimorfizme imaju značajno povišen rizik sa ponavljane gubitke trudnoće. Radi potvrde dobijenih rezultata potrebne su veće prospektivne studije.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in serbian population, Kombinovano prisustvo genskih polimorfizma faktora koagulacije XIII V34L i inhibitora plazminogen aktivatora 1 4G/5G značajno utiče na rizik od spontanog pobačaja u srpskoj populaciji",
volume = "39",
number = "2",
pages = "199-207",
doi = "10.2478/jomb-2019-0028"
}

Joksić, I., Miković, Ž., Filimonović, D., Munjas, J., Karadžov-Orlić, N., Egić, A.,& Joksić, G.. (2020). Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in serbian population. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(2), 199-207.
https://doi.org/10.2478/jomb-2019-0028

Joksić I, Miković Ž, Filimonović D, Munjas J, Karadžov-Orlić N, Egić A, Joksić G. Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in serbian population. in Journal of Medical Biochemistry. 2020;39(2):199-207.
doi:10.2478/jomb-2019-0028 .

Joksić, Ivana, Miković, Željko, Filimonović, Dejan, Munjas, Jelena, Karadžov-Orlić, Nataša, Egić, Amira, Joksić, Gordana, "Combined presence of coagulation factor XIII V34L and plasminogen activator inhibitor 1 4G/5G gene polymorphisms significantly contribute to recurrent pregnancy loss in serbian population" in Journal of Medical Biochemistry, 39, no. 2 (2020):199-207,
https://doi.org/10.2478/jomb-2019-0028 . .