TY  - JOUR
AU  - Dimić-Janjić, Sanja
AU  - Hoda, Mir Alireza
AU  - Milenković, Branislava
AU  - Kotur-Stevuljević, Jelena
AU  - Stjepanović, Mihailo
AU  - Gompelmann, Daniela
AU  - Janković, Jelena
AU  - Miljković, Milica
AU  - Milin-Lazović, Jelena
AU  - Đurđević, Nataša
AU  - Marić, Dragana
AU  - Milivojević, Ivan
AU  - Popević, Spasoje
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4612
AB  - Background: Inflammation, oxidative stress and an imbalance between proteases and protease inhibitors are recognized pathophysiological features of chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate serum levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in patients with COPD and to assess their relationship with lung function, symptom severity scores and recent acute exacerbations. Methods: In this observational cohort study, serum levels of MMP-9 and TIMP-1 and the MMP-9/TIMP-1 ratio in the peripheral blood of COPD patients with stable disease and healthy controls were determined, and their association with lung function (postbronchodilator spirometry, body plethysmography, single breath diffusion capacity for carbon monoxide), symptom severity scores (mMRC and CAT) and exacerbation history were assessed. Results: COPD patients (n = 98) had significantly higher levels of serum MMP-9 and TIMP-1 and a higher MMP-9/TIMP-1 ratio than healthy controls (n = 47) (p ≤ 0.001). The areas under the receiver operating characteristic curve for MMP-9, TIMP-1 and the MMP-9/TIMP-1 ratio for COPD diagnosis were 0.974, 0.961 and 0.910, respectively (all p < 0.05). MMP-9 and the MMP-9/TIMP-1 ratio were both negatively correlated with FVC, FEV1, FEV1/FVC, VC, and IC (all p < 0.05). For MMP-9, a positive correlation was found with RV/TLC% (p = 0.005), and a positive correlation was found for the MMP-9/TIMP-1 ratio with RV% and RV/TLC% (p = 0.013 and 0.002, respectively). Patients with COPD GOLD 3 and 4 presented greater MMP-9 levels and a greater MMP-9/TIMP-1 ratio compared to GOLD 1 and 2 patients (p ≤ 0.001). No correlation between diffusion capacity for carbon monoxide and number of acute exacerbations in the previous year was found. Conclusions: COPD patients have elevated serum levels of MMP-9 and TIMP-1 and MMP-9/TIMP-1 ratio. COPD patients have an imbalance between MMP-9 and TIMP-1 in favor of a pro-proteolytic environment, which overall indicates the importance of the MMP-9/TIMP-1 ratio as a potential biomarker for COPD diagnosis and severity.
PB  - BioMed Central Ltd
T2  - European Journal of Medical Research
T1  - The usefulness of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio for diagnosis and assessment of COPD severity
VL  - 28
IS  - 1
DO  - 10.1186/s40001-023-01094-7
ER  - 

@article{
author = "Dimić-Janjić, Sanja and Hoda, Mir Alireza and Milenković, Branislava and Kotur-Stevuljević, Jelena and Stjepanović, Mihailo and Gompelmann, Daniela and Janković, Jelena and Miljković, Milica and Milin-Lazović, Jelena and Đurđević, Nataša and Marić, Dragana and Milivojević, Ivan and Popević, Spasoje",
year = "2023",
abstract = "Background: Inflammation, oxidative stress and an imbalance between proteases and protease inhibitors are recognized pathophysiological features of chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate serum levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in patients with COPD and to assess their relationship with lung function, symptom severity scores and recent acute exacerbations. Methods: In this observational cohort study, serum levels of MMP-9 and TIMP-1 and the MMP-9/TIMP-1 ratio in the peripheral blood of COPD patients with stable disease and healthy controls were determined, and their association with lung function (postbronchodilator spirometry, body plethysmography, single breath diffusion capacity for carbon monoxide), symptom severity scores (mMRC and CAT) and exacerbation history were assessed. Results: COPD patients (n = 98) had significantly higher levels of serum MMP-9 and TIMP-1 and a higher MMP-9/TIMP-1 ratio than healthy controls (n = 47) (p ≤ 0.001). The areas under the receiver operating characteristic curve for MMP-9, TIMP-1 and the MMP-9/TIMP-1 ratio for COPD diagnosis were 0.974, 0.961 and 0.910, respectively (all p < 0.05). MMP-9 and the MMP-9/TIMP-1 ratio were both negatively correlated with FVC, FEV1, FEV1/FVC, VC, and IC (all p < 0.05). For MMP-9, a positive correlation was found with RV/TLC% (p = 0.005), and a positive correlation was found for the MMP-9/TIMP-1 ratio with RV% and RV/TLC% (p = 0.013 and 0.002, respectively). Patients with COPD GOLD 3 and 4 presented greater MMP-9 levels and a greater MMP-9/TIMP-1 ratio compared to GOLD 1 and 2 patients (p ≤ 0.001). No correlation between diffusion capacity for carbon monoxide and number of acute exacerbations in the previous year was found. Conclusions: COPD patients have elevated serum levels of MMP-9 and TIMP-1 and MMP-9/TIMP-1 ratio. COPD patients have an imbalance between MMP-9 and TIMP-1 in favor of a pro-proteolytic environment, which overall indicates the importance of the MMP-9/TIMP-1 ratio as a potential biomarker for COPD diagnosis and severity.",
publisher = "BioMed Central Ltd",
journal = "European Journal of Medical Research",
title = "The usefulness of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio for diagnosis and assessment of COPD severity",
volume = "28",
number = "1",
doi = "10.1186/s40001-023-01094-7"
}

Dimić-Janjić, S., Hoda, M. A., Milenković, B., Kotur-Stevuljević, J., Stjepanović, M., Gompelmann, D., Janković, J., Miljković, M., Milin-Lazović, J., Đurđević, N., Marić, D., Milivojević, I.,& Popević, S.. (2023). The usefulness of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio for diagnosis and assessment of COPD severity. in European Journal of Medical Research
BioMed Central Ltd., 28(1).
https://doi.org/10.1186/s40001-023-01094-7

Dimić-Janjić S, Hoda MA, Milenković B, Kotur-Stevuljević J, Stjepanović M, Gompelmann D, Janković J, Miljković M, Milin-Lazović J, Đurđević N, Marić D, Milivojević I, Popević S. The usefulness of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio for diagnosis and assessment of COPD severity. in European Journal of Medical Research. 2023;28(1).
doi:10.1186/s40001-023-01094-7 .

Dimić-Janjić, Sanja, Hoda, Mir Alireza, Milenković, Branislava, Kotur-Stevuljević, Jelena, Stjepanović, Mihailo, Gompelmann, Daniela, Janković, Jelena, Miljković, Milica, Milin-Lazović, Jelena, Đurđević, Nataša, Marić, Dragana, Milivojević, Ivan, Popević, Spasoje, "The usefulness of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio for diagnosis and assessment of COPD severity" in European Journal of Medical Research, 28, no. 1 (2023),
https://doi.org/10.1186/s40001-023-01094-7 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Milojević, Ana
AU  - Vladimirov, Sandra
AU  - Zdravković, Marija
AU  - Memon, Lidija
AU  - Brajković, Milica
AU  - Gardijan, Vera
AU  - Gojković, Tamara
AU  - Stefanović, Aleksandra
AU  - Miljković-Trailović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ninić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4322
AB  - Background and aims: Obstructive sleep apnea (OSA) is closely linked to obesity and related adverse metabolic changes, including dyslipidemia. However, it is not clear whether OSA is an independent contributing factor to dyslipidemia, or the observed association is a reflection of a concomitant presence of obesity. Additionally, dyslipidemia is usually evaluated through measurement of parameters of routine lipid status, while more precise evaluation of lipid homeostasis is rarely performed in OSA. In this study, we analyzed markers of cholesterol synthesis and absorption in patients with OSA with respect to the presence of obesity and the disease severity. Methods and results: This study enrolled 116 OSA patients. Concentrations of non-cholesterol sterols (NCS), measured by LC-MS/MS, were used as markers of cholesterol synthesis and absorption. Apnea-hypopnea index (AHI) and oxygen saturation (SaO2) were utilized as markers of OSA severity. Serum lipid status parameters were determined by routine enzymatic methods. Markers of cholesterol synthesis were increased (P = 0.005), whilst markers of cholesterol absorption decreased (P = 0.001) in obese OSA patients. Cholesterol synthesis/absorption ratio was elevated in obese subjects (P < 0.001). Concentration of cholesterol synthesis marker lathosterol was significantly higher in subjects with severe OSA (P = 0.014) and we observed a trend of decreased cholesterol absorption in these patients. AHI was revealed as an independent determinant of lathosterol concentration (P = 0.022). Conclusions: Our results suggest that the presence of obesity and severe forms of OSA is characterized by elevated endogenous cholesterol synthesis. AHI was singled out as an independent determinant of the serum level of cholesterol synthesis marker lathosterol.
PB  - Elsevier B.V.
T2  - Nutrition, Metabolism and Cardiovascular Diseases
T1  - Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity
VL  - 32
IS  - 12
SP  - 2848
EP  - 2857
DO  - 10.1016/j.numecd.2022.09.006
ER  - 

@article{
author = "Zeljković, Aleksandra and Milojević, Ana and Vladimirov, Sandra and Zdravković, Marija and Memon, Lidija and Brajković, Milica and Gardijan, Vera and Gojković, Tamara and Stefanović, Aleksandra and Miljković-Trailović, Milica and Spasojević-Kalimanovska, Vesna and Ninić, Ana",
year = "2022",
abstract = "Background and aims: Obstructive sleep apnea (OSA) is closely linked to obesity and related adverse metabolic changes, including dyslipidemia. However, it is not clear whether OSA is an independent contributing factor to dyslipidemia, or the observed association is a reflection of a concomitant presence of obesity. Additionally, dyslipidemia is usually evaluated through measurement of parameters of routine lipid status, while more precise evaluation of lipid homeostasis is rarely performed in OSA. In this study, we analyzed markers of cholesterol synthesis and absorption in patients with OSA with respect to the presence of obesity and the disease severity. Methods and results: This study enrolled 116 OSA patients. Concentrations of non-cholesterol sterols (NCS), measured by LC-MS/MS, were used as markers of cholesterol synthesis and absorption. Apnea-hypopnea index (AHI) and oxygen saturation (SaO2) were utilized as markers of OSA severity. Serum lipid status parameters were determined by routine enzymatic methods. Markers of cholesterol synthesis were increased (P = 0.005), whilst markers of cholesterol absorption decreased (P = 0.001) in obese OSA patients. Cholesterol synthesis/absorption ratio was elevated in obese subjects (P < 0.001). Concentration of cholesterol synthesis marker lathosterol was significantly higher in subjects with severe OSA (P = 0.014) and we observed a trend of decreased cholesterol absorption in these patients. AHI was revealed as an independent determinant of lathosterol concentration (P = 0.022). Conclusions: Our results suggest that the presence of obesity and severe forms of OSA is characterized by elevated endogenous cholesterol synthesis. AHI was singled out as an independent determinant of the serum level of cholesterol synthesis marker lathosterol.",
publisher = "Elsevier B.V.",
journal = "Nutrition, Metabolism and Cardiovascular Diseases",
title = "Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity",
volume = "32",
number = "12",
pages = "2848-2857",
doi = "10.1016/j.numecd.2022.09.006"
}

Zeljković, A., Milojević, A., Vladimirov, S., Zdravković, M., Memon, L., Brajković, M., Gardijan, V., Gojković, T., Stefanović, A., Miljković-Trailović, M., Spasojević-Kalimanovska, V.,& Ninić, A.. (2022). Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity. in Nutrition, Metabolism and Cardiovascular Diseases
Elsevier B.V.., 32(12), 2848-2857.
https://doi.org/10.1016/j.numecd.2022.09.006

Zeljković A, Milojević A, Vladimirov S, Zdravković M, Memon L, Brajković M, Gardijan V, Gojković T, Stefanović A, Miljković-Trailović M, Spasojević-Kalimanovska V, Ninić A. Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity. in Nutrition, Metabolism and Cardiovascular Diseases. 2022;32(12):2848-2857.
doi:10.1016/j.numecd.2022.09.006 .

Zeljković, Aleksandra, Milojević, Ana, Vladimirov, Sandra, Zdravković, Marija, Memon, Lidija, Brajković, Milica, Gardijan, Vera, Gojković, Tamara, Stefanović, Aleksandra, Miljković-Trailović, Milica, Spasojević-Kalimanovska, Vesna, Ninić, Ana, "Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity" in Nutrition, Metabolism and Cardiovascular Diseases, 32, no. 12 (2022):2848-2857,
https://doi.org/10.1016/j.numecd.2022.09.006 . .

TY  - JOUR
AU  - Perović-Blagojević, Iva
AU  - Vekić, Jelena
AU  - Macut, Đuro
AU  - Ignjatović, Svetlana
AU  - Miljković-Trailović, Milica
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Božić-Antić, Ivana
AU  - Bjekić-Macut, Jelica
AU  - Kastratović-Kotlica, Biljana
AU  - Andrić, Zoran
AU  - Ilić, Dušan
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3976
AB  - Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia
VL  - 128
IS  - 4
SP  - 604
EP  - 612
DO  - 10.1017/S0007114521003585
ER  - 

@article{
author = "Perović-Blagojević, Iva and Vekić, Jelena and Macut, Đuro and Ignjatović, Svetlana and Miljković-Trailović, Milica and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Božić-Antić, Ivana and Bjekić-Macut, Jelica and Kastratović-Kotlica, Biljana and Andrić, Zoran and Ilić, Dušan and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia",
volume = "128",
number = "4",
pages = "604-612",
doi = "10.1017/S0007114521003585"
}

Perović-Blagojević, I., Vekić, J., Macut, Đ., Ignjatović, S., Miljković-Trailović, M., Zeljković, A., Spasojević-Kalimanovska, V., Božić-Antić, I., Bjekić-Macut, J., Kastratović-Kotlica, B., Andrić, Z., Ilić, D.,& Kotur-Stevuljević, J.. (2022). Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition
Cambridge University Press., 128(4), 604-612.
https://doi.org/10.1017/S0007114521003585

Perović-Blagojević I, Vekić J, Macut Đ, Ignjatović S, Miljković-Trailović M, Zeljković A, Spasojević-Kalimanovska V, Božić-Antić I, Bjekić-Macut J, Kastratović-Kotlica B, Andrić Z, Ilić D, Kotur-Stevuljević J. Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition. 2022;128(4):604-612.
doi:10.1017/S0007114521003585 .

Perović-Blagojević, Iva, Vekić, Jelena, Macut, Đuro, Ignjatović, Svetlana, Miljković-Trailović, Milica, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Božić-Antić, Ivana, Bjekić-Macut, Jelica, Kastratović-Kotlica, Biljana, Andrić, Zoran, Ilić, Dušan, Kotur-Stevuljević, Jelena, "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia" in British Journal of Nutrition, 128, no. 4 (2022):604-612,
https://doi.org/10.1017/S0007114521003585 . .

TY  - JOUR
AU  - Perović-Blagojević, Iva
AU  - Vekić, Jelena
AU  - Macut, Đuro
AU  - Ignjatović, Svetlana
AU  - Miljković-Trailović, Milica
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Božić-Antić, Ivana
AU  - Bjekić-Macut, Jelica
AU  - Kastratović-Kotlica, Biljana
AU  - Andrić, Zoran
AU  - Ilić, Dušan
AU  - Kotur-Stevuljević, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3974
AB  - Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia
DO  - 10.1017/S0007114521003585
ER  - 

@article{
author = "Perović-Blagojević, Iva and Vekić, Jelena and Macut, Đuro and Ignjatović, Svetlana and Miljković-Trailović, Milica and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Božić-Antić, Ivana and Bjekić-Macut, Jelica and Kastratović-Kotlica, Biljana and Andrić, Zoran and Ilić, Dušan and Kotur-Stevuljević, Jelena",
year = "2021",
abstract = "Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia",
doi = "10.1017/S0007114521003585"
}

Perović-Blagojević, I., Vekić, J., Macut, Đ., Ignjatović, S., Miljković-Trailović, M., Zeljković, A., Spasojević-Kalimanovska, V., Božić-Antić, I., Bjekić-Macut, J., Kastratović-Kotlica, B., Andrić, Z., Ilić, D.,& Kotur-Stevuljević, J.. (2021). Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition
Cambridge University Press..
https://doi.org/10.1017/S0007114521003585

Perović-Blagojević I, Vekić J, Macut Đ, Ignjatović S, Miljković-Trailović M, Zeljković A, Spasojević-Kalimanovska V, Božić-Antić I, Bjekić-Macut J, Kastratović-Kotlica B, Andrić Z, Ilić D, Kotur-Stevuljević J. Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition. 2021;.
doi:10.1017/S0007114521003585 .

Perović-Blagojević, Iva, Vekić, Jelena, Macut, Đuro, Ignjatović, Svetlana, Miljković-Trailović, Milica, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Božić-Antić, Ivana, Bjekić-Macut, Jelica, Kastratović-Kotlica, Biljana, Andrić, Zoran, Ilić, Dušan, Kotur-Stevuljević, Jelena, "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia" in British Journal of Nutrition (2021),
https://doi.org/10.1017/S0007114521003585 . .

TY  - JOUR
AU  - Baralić, Katarina
AU  - Jorgovanović, Dragica
AU  - Živančević, Katarina
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Miljković, Milica
AU  - Kotur-Stevuljević, Jelena
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3762
AB  - The aim of this study was to: (i) determine and compare the capacity of bis (2 –ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), bisphenol A (BPA), and their mixture to produce testicular toxicity after the subacute exposure; (ii) explore the mechanisms behind the observed changes using in silico toxicogenomic approach. Male rats were randomly split into groups (n = 6): (1) Control (corn oil); (2) DEHP (50 mg/kg b.w./day); (3) DBP (50 mg/kg b.w./day); (4) BPA (25 mg/kg b.w./day); and (5) MIX (50 mg/kg b.w./day DEHP + 50 mg/kg b.w/day DBP + 25 mg/kg b.w./day BPA). Animals were sacrificed after 28 days of oral exposure, testes were extracted and prepared for histological assessments under the light microscope (haematoxylin and eosin staining) and redox status analysis. The Comparative Toxicogenomics Database (CTD; http://CTD.mdibl.org), Cytoscape software (https://cytoscape.org) and ToppGene Suite (https://toppgene.cchmc.org) were used for data-mining. Present pathohistological study has demonstrated more pronounced testicular toxicity of the MIX group (desquamated germinal epithelium cells, enlarged cells with hyperchromatic nuclei, multinucleated cell forms and intracytoplasmic vacuoles) in comparison with the single substances, while effects on redox status parameters were either more prominent, or present only in the MIX group. In silico investigation revealed 20 genes linked to male reproductive disorders, affected by all three investigated substances. Effects on metabolism, AhR pathway, apoptosis and oxidative stress could be singled out as the most probable mechanisms involved in the subacute DEHP, DBP and BPA mixture testicular toxicity, while the effect on oxidative stress parameters was confirmed by in vivo experiment.
PB  - Elsevier Ltd
T2  - Chemosphere
T1  - Combining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity
VL  - 267
DO  - 10.1016/j.chemosphere.2020.129296
ER  - 

@article{
author = "Baralić, Katarina and Jorgovanović, Dragica and Živančević, Katarina and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Miljković, Milica and Kotur-Stevuljević, Jelena and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2021",
abstract = "The aim of this study was to: (i) determine and compare the capacity of bis (2 –ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), bisphenol A (BPA), and their mixture to produce testicular toxicity after the subacute exposure; (ii) explore the mechanisms behind the observed changes using in silico toxicogenomic approach. Male rats were randomly split into groups (n = 6): (1) Control (corn oil); (2) DEHP (50 mg/kg b.w./day); (3) DBP (50 mg/kg b.w./day); (4) BPA (25 mg/kg b.w./day); and (5) MIX (50 mg/kg b.w./day DEHP + 50 mg/kg b.w/day DBP + 25 mg/kg b.w./day BPA). Animals were sacrificed after 28 days of oral exposure, testes were extracted and prepared for histological assessments under the light microscope (haematoxylin and eosin staining) and redox status analysis. The Comparative Toxicogenomics Database (CTD; http://CTD.mdibl.org), Cytoscape software (https://cytoscape.org) and ToppGene Suite (https://toppgene.cchmc.org) were used for data-mining. Present pathohistological study has demonstrated more pronounced testicular toxicity of the MIX group (desquamated germinal epithelium cells, enlarged cells with hyperchromatic nuclei, multinucleated cell forms and intracytoplasmic vacuoles) in comparison with the single substances, while effects on redox status parameters were either more prominent, or present only in the MIX group. In silico investigation revealed 20 genes linked to male reproductive disorders, affected by all three investigated substances. Effects on metabolism, AhR pathway, apoptosis and oxidative stress could be singled out as the most probable mechanisms involved in the subacute DEHP, DBP and BPA mixture testicular toxicity, while the effect on oxidative stress parameters was confirmed by in vivo experiment.",
publisher = "Elsevier Ltd",
journal = "Chemosphere",
title = "Combining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity",
volume = "267",
doi = "10.1016/j.chemosphere.2020.129296"
}

Baralić, K., Jorgovanović, D., Živančević, K., Buha-Đorđević, A., Antonijević-Miljaković, E., Miljković, M., Kotur-Stevuljević, J., Antonijević, B.,& Đukić-Ćosić, D.. (2021). Combining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity. in Chemosphere
Elsevier Ltd., 267.
https://doi.org/10.1016/j.chemosphere.2020.129296

Baralić K, Jorgovanović D, Živančević K, Buha-Đorđević A, Antonijević-Miljaković E, Miljković M, Kotur-Stevuljević J, Antonijević B, Đukić-Ćosić D. Combining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity. in Chemosphere. 2021;267.
doi:10.1016/j.chemosphere.2020.129296 .

Baralić, Katarina, Jorgovanović, Dragica, Živančević, Katarina, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Miljković, Milica, Kotur-Stevuljević, Jelena, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Combining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity" in Chemosphere, 267 (2021),
https://doi.org/10.1016/j.chemosphere.2020.129296 . .

TY  - JOUR
AU  - Vuković Dejanović, Vesna
AU  - Kotur-Stevuljević, Jelena
AU  - Vukašinović, Aleksandra
AU  - Miljković, Milica
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Hinić, Saša
AU  - Cerović, Milivoje
AU  - Stefanović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Memon, Lidija
AU  - Nešković, Aleksandar
AU  - Bogavac-Stanojević, Nataša
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3612
AB  - We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.
PB  - SAGE journals
T2  - Angiology
T1  - Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?
VL  - 71
IS  - 8
SP  - 713
EP  - 720
DO  - 10.1177/0003319720921724
ER  - 

@article{
author = "Vuković Dejanović, Vesna and Kotur-Stevuljević, Jelena and Vukašinović, Aleksandra and Miljković, Milica and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Spasojević-Kalimanovska, Vesna and Memon, Lidija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša",
year = "2020",
abstract = "We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.",
publisher = "SAGE journals",
journal = "Angiology",
title = "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?",
volume = "71",
number = "8",
pages = "713-720",
doi = "10.1177/0003319720921724"
}

Vuković Dejanović, V., Kotur-Stevuljević, J., Vukašinović, A., Miljković, M., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Spasojević-Kalimanovska, V., Memon, L., Nešković, A.,& Bogavac-Stanojević, N.. (2020). Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology
SAGE journals., 71(8), 713-720.
https://doi.org/10.1177/0003319720921724

Vuković Dejanović V, Kotur-Stevuljević J, Vukašinović A, Miljković M, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Spasojević-Kalimanovska V, Memon L, Nešković A, Bogavac-Stanojević N. Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology. 2020;71(8):713-720.
doi:10.1177/0003319720921724 .

Vuković Dejanović, Vesna, Kotur-Stevuljević, Jelena, Vukašinović, Aleksandra, Miljković, Milica, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Spasojević-Kalimanovska, Vesna, Memon, Lidija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?" in Angiology, 71, no. 8 (2020):713-720,
https://doi.org/10.1177/0003319720921724 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Miljković, Milica
AU  - Mandić-Marković, Vesna
AU  - Stanimirović, Srđan
AU  - Damnjanović Pažin, Barbara
AU  - Spasić, Slavíca
AU  - Spasojević-Kalimanovska, Vesna
AU  - Karadžov-Orlić, Nataša
AU  - Miković, Željko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3626
AB  - Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction
VL  - 81
SP  - 34
EP  - 40
DO  - 10.1016/j.clinbiochem.2020.05.003
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Banjac, Gorica and Cabunac, Petar and Miljković, Milica and Mandić-Marković, Vesna and Stanimirović, Srđan and Damnjanović Pažin, Barbara and Spasić, Slavíca and Spasojević-Kalimanovska, Vesna and Karadžov-Orlić, Nataša and Miković, Željko",
year = "2020",
abstract = "Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction",
volume = "81",
pages = "34-40",
doi = "10.1016/j.clinbiochem.2020.05.003"
}

Ardalić, D., Stefanović, A., Banjac, G., Cabunac, P., Miljković, M., Mandić-Marković, V., Stanimirović, S., Damnjanović Pažin, B., Spasić, S., Spasojević-Kalimanovska, V., Karadžov-Orlić, N.,& Miković, Ž.. (2020). Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry
Elsevier., 81, 34-40.
https://doi.org/10.1016/j.clinbiochem.2020.05.003

Ardalić D, Stefanović A, Banjac G, Cabunac P, Miljković M, Mandić-Marković V, Stanimirović S, Damnjanović Pažin B, Spasić S, Spasojević-Kalimanovska V, Karadžov-Orlić N, Miković Ž. Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry. 2020;81:34-40.
doi:10.1016/j.clinbiochem.2020.05.003 .

Ardalić, Daniela, Stefanović, Aleksandra, Banjac, Gorica, Cabunac, Petar, Miljković, Milica, Mandić-Marković, Vesna, Stanimirović, Srđan, Damnjanović Pažin, Barbara, Spasić, Slavíca, Spasojević-Kalimanovska, Vesna, Karadžov-Orlić, Nataša, Miković, Željko, "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction" in Clinical Biochemistry, 81 (2020):34-40,
https://doi.org/10.1016/j.clinbiochem.2020.05.003 . .

TY  - JOUR
AU  - Janać, Jelena
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Dimitrijević-Srećković, Vesna
AU  - Vekić, Jelena
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Ivanišević, Jasmina
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3803
AB  - Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.
PB  - Oxford University Press
T2  - Lab Medicine
T1  - Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals
VL  - 51
IS  - 1
SP  - 24
EP  - 33
DO  - 10.1093/labmed/lmz017
ER  - 

@article{
author = "Janać, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Dimitrijević-Srećković, Vesna and Vekić, Jelena and Miljković, Milica and Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.",
publisher = "Oxford University Press",
journal = "Lab Medicine",
title = "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals",
volume = "51",
number = "1",
pages = "24-33",
doi = "10.1093/labmed/lmz017"
}

Janać, J., Zeljković, A., Jelić-Ivanović, Z., Dimitrijević-Srećković, V., Vekić, J., Miljković, M., Stefanović, A., Kotur-Stevuljević, J., Ivanišević, J.,& Spasojević-Kalimanovska, V.. (2020). Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine
Oxford University Press., 51(1), 24-33.
https://doi.org/10.1093/labmed/lmz017

Janać J, Zeljković A, Jelić-Ivanović Z, Dimitrijević-Srećković V, Vekić J, Miljković M, Stefanović A, Kotur-Stevuljević J, Ivanišević J, Spasojević-Kalimanovska V. Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine. 2020;51(1):24-33.
doi:10.1093/labmed/lmz017 .

Janać, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Dimitrijević-Srećković, Vesna, Vekić, Jelena, Miljković, Milica, Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Spasojević-Kalimanovska, Vesna, "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals" in Lab Medicine, 51, no. 1 (2020):24-33,
https://doi.org/10.1093/labmed/lmz017 . .

TY  - JOUR
AU  - Vekić, Jelena
AU  - Ivanišević, Jasmina
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Bogavac-Stanojević, Nataša
AU  - Mihajlović, Marija
AU  - Janać, Jelena
AU  - Vujčić, Sanja
AU  - Miljković, Milica
AU  - Zujović, Dejan
AU  - Kotur-Stevuljević, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3746
AB  - Background:Propolis  and  N-acetylcysteine  have  positiveimpact  on  respiratory  tract  health.  Also,  it  has  been  sug-gested that they have beneficial effects on serum lipid andoxidative  stress  status,  but  the  available  data  are  limitedand  mostly  gained  from  animal  models.  In  this  study  weevaluated the effects of propolis and N-acetylcysteine sup-plementation  (PropoMucil®)  on  lipid  status,  lipoproteinsubclasses  distribution  and  paraoxonase  1  activity  in  sub-jects with acute respiratory infection.Methods:Twenty  subjects  with  acute  respiratory  infectionwere included. PropoMucil®granules for oral solution (80mg of dry propolis extract and 200 mg of N-acetylcysteine)were administered tree times per day for ten days. Serumlipid  profile,  paraoxonase  1  activity  and  low-density  andhigh-density  lipoprotein  size  and  subclasses  distributionwere assessed at baseline and after supplementation.Results:Following ten days of supplementation lipid statusremained  unchanged,  but  a  significant  increase  of  low-density lipoprotein particle size and proportion of high-den-sity   lipoprotein   3a   particles   were   found   (P<0.05).Moreover, supplementation with PropoMucil®significantlyimproved  high-density  lipoprotein  particles  distribution, particularly  in  those  who  smoke.  There  was  a  moderateincrease  of  paraoxonase  1  activity,  but  without  statisticalsignificance.Conclusions:The presented study demonstrated that short-term  supplementation  with  PropoMucil®has  beneficialeffects  on  low-density  and  high-density  lipoprotein  sub-classes  distribution  and  paraoxonase  1  activity  in  subjectswith  acute  respiratory  infection  particularly  in  those  whosmoke.
AB  - Uvod: Propolis i N-acetilcistein pozitivno utiču na zdravlje disajnih puteva. Takođe, sugeriše se da oni imaju blagotvorno dejstvo na lipidni i oksidativno-stresni status, ali podaci su ograničeni i dobijeni uglavnom na životinjskim modelima. U ovom istraživanju, procenili smo kombinovani efekat propolisa i N-acetilcisteina (PropoMucil®) na status lipida, raspodelu supklasa lipoproteina i aktivnost paraoksonaze 1 kod pacijenata sa akutnom respiratornom infekcijom (ARI). Metode: Uključeno je 20 ispitanika sa akutnom respiratornom infekcijom. Oralni rastvor praška PropoMucil® (80 mg suvog ekstrakta propolisa i 200 mg N-acetilcisteina) je aplikovan 3 puta dnevno tokom 10 dana. Serumski lipidni profil, aktivnost praoksonaze 1 i veličina i raspodela supfrakcija lipoproteina niske gustine i lipoproteina visoke gustine su određeni pre i nakon suplementacije. Rezultati: Nakon deset dana suplementacije, lipidni status je ostao nepromenjen, ali je utvrđeno značajno povećanje veličine lipoproteina niske gustine i relativnog udela lipoproteina visoke gustine 3a (P <0,05). Pored toga, suplementacija PropoMucil®-om značajno je poboljšala distribuciju lipoproteinskih čestica visoke gustine, posebno kod pacijenata koji puše. Došlo je do umerenog porasta aktivnosti paraoksonaze 1, ali bez statističke značajnosti. Zaključak: Studija je pokazala da kratkotrajna suplementacija PropoMucil®-om ima korisne efekte na distribuciju supklasa lipoproteina niske i visoke gustine i aktivnost paraoksonaze 1 kod ispitanika sa akutnom respiratornom infekcijom, posebno kod onih koji puše.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection
T1  - Efekat suplementacije propolisom i N-acetilcisteinom na raspodelu lipoproteinskih supklasa i aktivnosti paraoksonaze 1 u osoba sa akutnom respiratornom infekcijom
VL  - 39
IS  - 4
SP  - 467
EP  - 473
DO  - 10.5937/jomb0-24695
ER  - 

@article{
author = "Vekić, Jelena and Ivanišević, Jasmina and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Mihajlović, Marija and Janać, Jelena and Vujčić, Sanja and Miljković, Milica and Zujović, Dejan and Kotur-Stevuljević, Jelena",
year = "2020",
abstract = "Background:Propolis  and  N-acetylcysteine  have  positiveimpact  on  respiratory  tract  health.  Also,  it  has  been  sug-gested that they have beneficial effects on serum lipid andoxidative  stress  status,  but  the  available  data  are  limitedand  mostly  gained  from  animal  models.  In  this  study  weevaluated the effects of propolis and N-acetylcysteine sup-plementation  (PropoMucil®)  on  lipid  status,  lipoproteinsubclasses  distribution  and  paraoxonase  1  activity  in  sub-jects with acute respiratory infection.Methods:Twenty  subjects  with  acute  respiratory  infectionwere included. PropoMucil®granules for oral solution (80mg of dry propolis extract and 200 mg of N-acetylcysteine)were administered tree times per day for ten days. Serumlipid  profile,  paraoxonase  1  activity  and  low-density  andhigh-density  lipoprotein  size  and  subclasses  distributionwere assessed at baseline and after supplementation.Results:Following ten days of supplementation lipid statusremained  unchanged,  but  a  significant  increase  of  low-density lipoprotein particle size and proportion of high-den-sity   lipoprotein   3a   particles   were   found   (P<0.05).Moreover, supplementation with PropoMucil®significantlyimproved  high-density  lipoprotein  particles  distribution, particularly  in  those  who  smoke.  There  was  a  moderateincrease  of  paraoxonase  1  activity,  but  without  statisticalsignificance.Conclusions:The presented study demonstrated that short-term  supplementation  with  PropoMucil®has  beneficialeffects  on  low-density  and  high-density  lipoprotein  sub-classes  distribution  and  paraoxonase  1  activity  in  subjectswith  acute  respiratory  infection  particularly  in  those  whosmoke., Uvod: Propolis i N-acetilcistein pozitivno utiču na zdravlje disajnih puteva. Takođe, sugeriše se da oni imaju blagotvorno dejstvo na lipidni i oksidativno-stresni status, ali podaci su ograničeni i dobijeni uglavnom na životinjskim modelima. U ovom istraživanju, procenili smo kombinovani efekat propolisa i N-acetilcisteina (PropoMucil®) na status lipida, raspodelu supklasa lipoproteina i aktivnost paraoksonaze 1 kod pacijenata sa akutnom respiratornom infekcijom (ARI). Metode: Uključeno je 20 ispitanika sa akutnom respiratornom infekcijom. Oralni rastvor praška PropoMucil® (80 mg suvog ekstrakta propolisa i 200 mg N-acetilcisteina) je aplikovan 3 puta dnevno tokom 10 dana. Serumski lipidni profil, aktivnost praoksonaze 1 i veličina i raspodela supfrakcija lipoproteina niske gustine i lipoproteina visoke gustine su određeni pre i nakon suplementacije. Rezultati: Nakon deset dana suplementacije, lipidni status je ostao nepromenjen, ali je utvrđeno značajno povećanje veličine lipoproteina niske gustine i relativnog udela lipoproteina visoke gustine 3a (P <0,05). Pored toga, suplementacija PropoMucil®-om značajno je poboljšala distribuciju lipoproteinskih čestica visoke gustine, posebno kod pacijenata koji puše. Došlo je do umerenog porasta aktivnosti paraoksonaze 1, ali bez statističke značajnosti. Zaključak: Studija je pokazala da kratkotrajna suplementacija PropoMucil®-om ima korisne efekte na distribuciju supklasa lipoproteina niske i visoke gustine i aktivnost paraoksonaze 1 kod ispitanika sa akutnom respiratornom infekcijom, posebno kod onih koji puše.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection, Efekat suplementacije propolisom i N-acetilcisteinom na raspodelu lipoproteinskih supklasa i aktivnosti paraoksonaze 1 u osoba sa akutnom respiratornom infekcijom",
volume = "39",
number = "4",
pages = "467-473",
doi = "10.5937/jomb0-24695"
}

Vekić, J., Ivanišević, J., Zeljković, A., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N., Mihajlović, M., Janać, J., Vujčić, S., Miljković, M., Zujović, D.,& Kotur-Stevuljević, J.. (2020). Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(4), 467-473.
https://doi.org/10.5937/jomb0-24695

Vekić J, Ivanišević J, Zeljković A, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Mihajlović M, Janać J, Vujčić S, Miljković M, Zujović D, Kotur-Stevuljević J. Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection. in Journal of Medical Biochemistry. 2020;39(4):467-473.
doi:10.5937/jomb0-24695 .

Vekić, Jelena, Ivanišević, Jasmina, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Mihajlović, Marija, Janać, Jelena, Vujčić, Sanja, Miljković, Milica, Zujović, Dejan, Kotur-Stevuljević, Jelena, "Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection" in Journal of Medical Biochemistry, 39, no. 4 (2020):467-473,
https://doi.org/10.5937/jomb0-24695 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Mihajlović, Marija
AU  - Stefanović, Aleksandra
AU  - Zeljković, D.
AU  - Trifunović, B.
AU  - Miljković, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Vekić, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3739
AB  - Aim: Despite many efforts, reliable biomarkers for the prediction and diagnosis of colorectal cancer (CRC) are still missing. Insulin-like growth factor 1 (IGF-1) and E-cadherin are recognized as potential biomarkers, but their diagnostic capacity is largely unexplored in CRC. The aim of this work is to investigate IGF-1 and E-cadherin levels with respect to various characteristics of CRC and to estimate their diagnostic potential. Method: Seventy CRC patients and 75 healthy individuals were enrolled. IGF-1 and E-cadherin were determined using enzyme-linked immunosorbent assay. The predictive and diagnostic capacities of IGF-1 and E-cadherin were estimated by logistic regression analysis and by determination of the area under the receiver operating characteristic (ROC) curve (AUC). Results: Concentrations of IGF-1 were lower (P = 0.019) while levels of E-cadherin were higher (P < 0.001) in CRC patients than in controls. IGF-1 concentration decreased in parallel with age and progression of CRC (P = 0.023). Also, IGF-1 was higher in men with CRC than in women (P = 0.003). E-cadherin levels were unaffected by variations in either anthropometric characteristics of CRC patients, or localization, grade and stage of the tumour. Both IGF-1 and E-cadherin were independently associated with CRC (P = 0.040; P < 0.001, respectively). The diagnostic accuracy of IGF-1 was estimated as acceptable (AUC = 0.757; P < 0.001), while the diagnostic accuracy of E-cadherin was outstanding (AUC = 0.954; P < 0.001). Conclusions: Decreased IGF-1 and increased E-cadherin levels were found in CRC patients. IGF-1, but not E-cadherin, concentrations differed according to age, gender and stage of CRC. Both markers were independently associated with the presence of the disease, while E-cadherin demonstrated high diagnostic accuracy.
PB  - Blackwell Publishing Ltd
T2  - Colorectal Disease
T1  - Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer
DO  - 10.1111/codi.15360
ER  - 

@article{
author = "Zeljković, Aleksandra and Mihajlović, Marija and Stefanović, Aleksandra and Zeljković, D. and Trifunović, B. and Miljković, Milica and Spasojević-Kalimanovska, Vesna and Vekić, Jelena",
year = "2020",
abstract = "Aim: Despite many efforts, reliable biomarkers for the prediction and diagnosis of colorectal cancer (CRC) are still missing. Insulin-like growth factor 1 (IGF-1) and E-cadherin are recognized as potential biomarkers, but their diagnostic capacity is largely unexplored in CRC. The aim of this work is to investigate IGF-1 and E-cadherin levels with respect to various characteristics of CRC and to estimate their diagnostic potential. Method: Seventy CRC patients and 75 healthy individuals were enrolled. IGF-1 and E-cadherin were determined using enzyme-linked immunosorbent assay. The predictive and diagnostic capacities of IGF-1 and E-cadherin were estimated by logistic regression analysis and by determination of the area under the receiver operating characteristic (ROC) curve (AUC). Results: Concentrations of IGF-1 were lower (P = 0.019) while levels of E-cadherin were higher (P < 0.001) in CRC patients than in controls. IGF-1 concentration decreased in parallel with age and progression of CRC (P = 0.023). Also, IGF-1 was higher in men with CRC than in women (P = 0.003). E-cadherin levels were unaffected by variations in either anthropometric characteristics of CRC patients, or localization, grade and stage of the tumour. Both IGF-1 and E-cadherin were independently associated with CRC (P = 0.040; P < 0.001, respectively). The diagnostic accuracy of IGF-1 was estimated as acceptable (AUC = 0.757; P < 0.001), while the diagnostic accuracy of E-cadherin was outstanding (AUC = 0.954; P < 0.001). Conclusions: Decreased IGF-1 and increased E-cadherin levels were found in CRC patients. IGF-1, but not E-cadherin, concentrations differed according to age, gender and stage of CRC. Both markers were independently associated with the presence of the disease, while E-cadherin demonstrated high diagnostic accuracy.",
publisher = "Blackwell Publishing Ltd",
journal = "Colorectal Disease",
title = "Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer",
doi = "10.1111/codi.15360"
}

Zeljković, A., Mihajlović, M., Stefanović, A., Zeljković, D., Trifunović, B., Miljković, M., Spasojević-Kalimanovska, V.,& Vekić, J.. (2020). Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer. in Colorectal Disease
Blackwell Publishing Ltd..
https://doi.org/10.1111/codi.15360

Zeljković A, Mihajlović M, Stefanović A, Zeljković D, Trifunović B, Miljković M, Spasojević-Kalimanovska V, Vekić J. Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer. in Colorectal Disease. 2020;.
doi:10.1111/codi.15360 .

Zeljković, Aleksandra, Mihajlović, Marija, Stefanović, Aleksandra, Zeljković, D., Trifunović, B., Miljković, Milica, Spasojević-Kalimanovska, Vesna, Vekić, Jelena, "Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer" in Colorectal Disease (2020),
https://doi.org/10.1111/codi.15360 . .

TY  - JOUR
AU  - Kotur-Stevuljević, Jelena
AU  - Vekić, Jelena
AU  - Stefanović, Aleksandra
AU  - Zeljković, Aleksandra
AU  - Ninić, Ana
AU  - Ivanišević, Jasmina
AU  - Miljković, Milica
AU  - Sopić, Miron
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Spasić, Slavica
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3615
AB  - A direct and an indirect relationship between paraoxonase 1 (PON1) and atherosclerosis exists. Given PON1's physical location within high-density lipoprotein (HDL) particles and its recognized enzyme activity, it is certainly reasonable to suggest that PON1 facilitates the antiatherogenic nature of HDL particles. PON1 also plays a role in regulating reverse cholesterol transport, antioxidative, anti-inflammatory, antiapoptotic, vasodilative, and antithrombotic activities and several endothelial cell functions. HDL dysfunctionality is a more recent issue and seems to be centered on pathological conditions affecting HDL structure and size profiles. This review is focused on the role of PON1 status in different atherosclerosis-related diseases that we have studied over the last twenty years (coronary heart disease, acute ischemic stroke, diabetes mellitus type 2, end-stage renal disease, chronic obstructive pulmonary disease, and sarcoidosis) with the aim to determine the true value of PON1 as a biomarker. The role of PON1 in cancer is also covered, as risk factors and mechanisms underlying both atherosclerosis and cancer share common features. © 2019 International Union of Biochemistry and Molecular Biology
PB  - Blackwell Publishing Inc.
T2  - BioFactors
T1  - Paraoxonase 1 and atherosclerosis-related diseases
VL  - 46
IS  - 2
SP  - 193
EP  - 205
DO  - 10.1002/biof.1549
ER  - 

@article{
author = "Kotur-Stevuljević, Jelena and Vekić, Jelena and Stefanović, Aleksandra and Zeljković, Aleksandra and Ninić, Ana and Ivanišević, Jasmina and Miljković, Milica and Sopić, Miron and Munjas, Jelena and Mihajlović, Marija and Spasić, Slavica and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "A direct and an indirect relationship between paraoxonase 1 (PON1) and atherosclerosis exists. Given PON1's physical location within high-density lipoprotein (HDL) particles and its recognized enzyme activity, it is certainly reasonable to suggest that PON1 facilitates the antiatherogenic nature of HDL particles. PON1 also plays a role in regulating reverse cholesterol transport, antioxidative, anti-inflammatory, antiapoptotic, vasodilative, and antithrombotic activities and several endothelial cell functions. HDL dysfunctionality is a more recent issue and seems to be centered on pathological conditions affecting HDL structure and size profiles. This review is focused on the role of PON1 status in different atherosclerosis-related diseases that we have studied over the last twenty years (coronary heart disease, acute ischemic stroke, diabetes mellitus type 2, end-stage renal disease, chronic obstructive pulmonary disease, and sarcoidosis) with the aim to determine the true value of PON1 as a biomarker. The role of PON1 in cancer is also covered, as risk factors and mechanisms underlying both atherosclerosis and cancer share common features. © 2019 International Union of Biochemistry and Molecular Biology",
publisher = "Blackwell Publishing Inc.",
journal = "BioFactors",
title = "Paraoxonase 1 and atherosclerosis-related diseases",
volume = "46",
number = "2",
pages = "193-205",
doi = "10.1002/biof.1549"
}

Kotur-Stevuljević, J., Vekić, J., Stefanović, A., Zeljković, A., Ninić, A., Ivanišević, J., Miljković, M., Sopić, M., Munjas, J., Mihajlović, M., Spasić, S., Jelić-Ivanović, Z.,& Spasojević-Kalimanovska, V.. (2020). Paraoxonase 1 and atherosclerosis-related diseases. in BioFactors
Blackwell Publishing Inc.., 46(2), 193-205.
https://doi.org/10.1002/biof.1549

Kotur-Stevuljević J, Vekić J, Stefanović A, Zeljković A, Ninić A, Ivanišević J, Miljković M, Sopić M, Munjas J, Mihajlović M, Spasić S, Jelić-Ivanović Z, Spasojević-Kalimanovska V. Paraoxonase 1 and atherosclerosis-related diseases. in BioFactors. 2020;46(2):193-205.
doi:10.1002/biof.1549 .

Kotur-Stevuljević, Jelena, Vekić, Jelena, Stefanović, Aleksandra, Zeljković, Aleksandra, Ninić, Ana, Ivanišević, Jasmina, Miljković, Milica, Sopić, Miron, Munjas, Jelena, Mihajlović, Marija, Spasić, Slavica, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, "Paraoxonase 1 and atherosclerosis-related diseases" in BioFactors, 46, no. 2 (2020):193-205,
https://doi.org/10.1002/biof.1549 . .

TY  - JOUR
AU  - Vladimirov, Sandra
AU  - Zeljković, Aleksandra
AU  - Gojković, Tamara
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3623
AB  - Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
AB  - Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer
T1  - Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa
VL  - 39
IS  - 3
SP  - 318
EP  - 327
DO  - 10.2478/jomb-2019-0047
ER  - 

@article{
author = "Vladimirov, Sandra and Zeljković, Aleksandra and Gojković, Tamara and Miljković, Milica and Stefanović, Aleksandra and Zeljković, Dejan and Trifunović, Bratislav and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor., Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer, Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa",
volume = "39",
number = "3",
pages = "318-327",
doi = "10.2478/jomb-2019-0047"
}

Vladimirov, S., Zeljković, A., Gojković, T., Miljković, M., Stefanović, A., Zeljković, D., Trifunović, B.,& Spasojević-Kalimanovska, V.. (2020). Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 318-327.
https://doi.org/10.2478/jomb-2019-0047

Vladimirov S, Zeljković A, Gojković T, Miljković M, Stefanović A, Zeljković D, Trifunović B, Spasojević-Kalimanovska V. Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry. 2020;39(3):318-327.
doi:10.2478/jomb-2019-0047 .

Vladimirov, Sandra, Zeljković, Aleksandra, Gojković, Tamara, Miljković, Milica, Stefanović, Aleksandra, Zeljković, Dejan, Trifunović, Bratislav, Spasojević-Kalimanovska, Vesna, "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer" in Journal of Medical Biochemistry, 39, no. 3 (2020):318-327,
https://doi.org/10.2478/jomb-2019-0047 . .

TY  - JOUR
AU  - Ninić, Ana
AU  - Bogavac-Stanojević, Nataša
AU  - Sopić, Miron
AU  - Munjas, Jelena
AU  - Kotur-Stevuljević, Jelena
AU  - Miljković, Milica
AU  - Gojković, Tamara
AU  - Kalimanovska-Oštrić, Dimitra
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3339
AB  - Background: Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression could be modulated by oxidative stress in CAD patients. Methods: This study included 77 CAD patients and 31 apparently healthy persons. Serum lipid levels, high sensitivity C-reactive protein (hsCRP), total antioxidant status (TAS) and thiobarbituric acid-reacting substances (TBARS) were measured. SOD isoenzymes gene expression was determined in peripheral blood mononuclear cells using quantitative polymerase chain reaction. Results: Mn SOD messenger ribonucleic acid (mRNA) levels were significantly lower in CAD patients than in controls (p=0.011), while Cu/Zn SOD mRNA levels did not change significantly between tested groups (p=0.091). We found significantly lower high-density lipoprotein-cholesterol (HDL-c) (p lt 0.001) and TAS (p lt 0.001) levels and significantly higher hsCRP (p=0.002) and TBARS (p lt 0.001) in CAD patients than in controls. There were significant positive correlations between TAS and Mn SOD mRNA (rho=0.243, p=0.020) and TAS and Cu/Zn SOD mRNA (rho=0.359, p lt 0.001). TBARS negatively correlated only with Cu/Zn SOD mRNA (rho=-0.215, p=0.040). TAS levels remained independent predictor for Mn SOD mRNA levels (OR = 2.995, p=0.034). Conclusions: Results of this study showed that Mn SOD gene expression were decreased in CAD patients compared to controls and can be modulated by nonenzymatic antioxidant status in blood.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease
VL  - 38
IS  - 3
SP  - 284
EP  - 291
DO  - 10.2478/jomb-2018-0041
ER  - 

@article{
author = "Ninić, Ana and Bogavac-Stanojević, Nataša and Sopić, Miron and Munjas, Jelena and Kotur-Stevuljević, Jelena and Miljković, Milica and Gojković, Tamara and Kalimanovska-Oštrić, Dimitra and Spasojević-Kalimanovska, Vesna",
year = "2019",
abstract = "Background: Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression could be modulated by oxidative stress in CAD patients. Methods: This study included 77 CAD patients and 31 apparently healthy persons. Serum lipid levels, high sensitivity C-reactive protein (hsCRP), total antioxidant status (TAS) and thiobarbituric acid-reacting substances (TBARS) were measured. SOD isoenzymes gene expression was determined in peripheral blood mononuclear cells using quantitative polymerase chain reaction. Results: Mn SOD messenger ribonucleic acid (mRNA) levels were significantly lower in CAD patients than in controls (p=0.011), while Cu/Zn SOD mRNA levels did not change significantly between tested groups (p=0.091). We found significantly lower high-density lipoprotein-cholesterol (HDL-c) (p lt 0.001) and TAS (p lt 0.001) levels and significantly higher hsCRP (p=0.002) and TBARS (p lt 0.001) in CAD patients than in controls. There were significant positive correlations between TAS and Mn SOD mRNA (rho=0.243, p=0.020) and TAS and Cu/Zn SOD mRNA (rho=0.359, p lt 0.001). TBARS negatively correlated only with Cu/Zn SOD mRNA (rho=-0.215, p=0.040). TAS levels remained independent predictor for Mn SOD mRNA levels (OR = 2.995, p=0.034). Conclusions: Results of this study showed that Mn SOD gene expression were decreased in CAD patients compared to controls and can be modulated by nonenzymatic antioxidant status in blood.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease",
volume = "38",
number = "3",
pages = "284-291",
doi = "10.2478/jomb-2018-0041"
}

Ninić, A., Bogavac-Stanojević, N., Sopić, M., Munjas, J., Kotur-Stevuljević, J., Miljković, M., Gojković, T., Kalimanovska-Oštrić, D.,& Spasojević-Kalimanovska, V.. (2019). Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 38(3), 284-291.
https://doi.org/10.2478/jomb-2018-0041

Ninić A, Bogavac-Stanojević N, Sopić M, Munjas J, Kotur-Stevuljević J, Miljković M, Gojković T, Kalimanovska-Oštrić D, Spasojević-Kalimanovska V. Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease. in Journal of Medical Biochemistry. 2019;38(3):284-291.
doi:10.2478/jomb-2018-0041 .

Ninić, Ana, Bogavac-Stanojević, Nataša, Sopić, Miron, Munjas, Jelena, Kotur-Stevuljević, Jelena, Miljković, Milica, Gojković, Tamara, Kalimanovska-Oštrić, Dimitra, Spasojević-Kalimanovska, Vesna, "Superoxide dismutase isoenzymes gene expression in peripheral blood mononuclear cells in patients with coronary artery disease" in Journal of Medical Biochemistry, 38, no. 3 (2019):284-291,
https://doi.org/10.2478/jomb-2018-0041 . .

TY  - JOUR
AU  - Janać, Jelena
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Dimitrijević-Srećković, Vesna
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Munjas, Jelena
AU  - Vekić, Jelena
AU  - Kotur-Stevuljević, Jelena
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3293
AB  - Background: Non-alcoholic fatty liver disease is a frequent ailment with known complications, including those within the cardiovascular system. Associations between several indicators of high-density lipoprotein metabolism and function with clinical and laboratory parameters for the assessment of fatty liver index, a surrogate marker of non-alcoholic fatty liver disease, were evaluated. Methods: The study comprised 130 patients classified according to fatty liver index values: fatty liver index  lt  30, fatty liver index 30–59 (the intermediate group) and fatty liver index ⩾ 60. Lecithin–cholesterol acyltransferase and cholesteryl ester transfer protein activities were determined. Paraoxonase 1 concentration and its activity, paraoxonase 3 concentration and high-density lipoprotein subclass distribution were assessed. Results: Increased lecithin–cholesterol acyltransferase activity correlated with increased fatty liver index (P  lt  0.001). Paraoxonase 3 concentration was lower in the fatty liver index ⩾ 60 group compared with the fatty liver index  lt  30 group (P  lt  0.05). Cholesteryl ester transfer protein activity, paraoxonase 1 concentration and its activity did not significantly differ across the fatty liver index groups. The relative proportion of small-sized high-density lipoprotein 3 subclass was higher in the fatty liver index ⩾ 60 group compared with the other two fatty liver index groups (P  lt  0.01). Lecithin–cholesterol acyltransferase activity positively associated with the fatty liver index ⩾ 60 group and remained significant after adjustment for other potential confounders. Only the triglyceride concentration remained significantly associated with lecithin–cholesterol acyltransferase activity when the parameters that constitute the fatty liver index equation were examined. Conclusions: Higher lecithin–cholesterol acyltransferase activity is associated with elevated fatty liver index values. Significant independent association between triglycerides and lecithin–cholesterol acyltransferase activity might indicate a role of hypertriglyceridaemia in alterations of lecithin–cholesterol acyltransferase activity in individuals with elevated fatty liver index.
PB  - Sage Publications Ltd
T2  - Annals of Clinical Biochemistry
T1  - The association between lecithin–cholesterol acyltransferase activity and fatty liver index
VL  - 56
IS  - 5
SP  - 583
EP  - 592
DO  - 10.1177/0004563219853596
ER  - 

@article{
author = "Janać, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Dimitrijević-Srećković, Vesna and Miljković, Milica and Stefanović, Aleksandra and Munjas, Jelena and Vekić, Jelena and Kotur-Stevuljević, Jelena and Spasojević-Kalimanovska, Vesna",
year = "2019",
abstract = "Background: Non-alcoholic fatty liver disease is a frequent ailment with known complications, including those within the cardiovascular system. Associations between several indicators of high-density lipoprotein metabolism and function with clinical and laboratory parameters for the assessment of fatty liver index, a surrogate marker of non-alcoholic fatty liver disease, were evaluated. Methods: The study comprised 130 patients classified according to fatty liver index values: fatty liver index  lt  30, fatty liver index 30–59 (the intermediate group) and fatty liver index ⩾ 60. Lecithin–cholesterol acyltransferase and cholesteryl ester transfer protein activities were determined. Paraoxonase 1 concentration and its activity, paraoxonase 3 concentration and high-density lipoprotein subclass distribution were assessed. Results: Increased lecithin–cholesterol acyltransferase activity correlated with increased fatty liver index (P  lt  0.001). Paraoxonase 3 concentration was lower in the fatty liver index ⩾ 60 group compared with the fatty liver index  lt  30 group (P  lt  0.05). Cholesteryl ester transfer protein activity, paraoxonase 1 concentration and its activity did not significantly differ across the fatty liver index groups. The relative proportion of small-sized high-density lipoprotein 3 subclass was higher in the fatty liver index ⩾ 60 group compared with the other two fatty liver index groups (P  lt  0.01). Lecithin–cholesterol acyltransferase activity positively associated with the fatty liver index ⩾ 60 group and remained significant after adjustment for other potential confounders. Only the triglyceride concentration remained significantly associated with lecithin–cholesterol acyltransferase activity when the parameters that constitute the fatty liver index equation were examined. Conclusions: Higher lecithin–cholesterol acyltransferase activity is associated with elevated fatty liver index values. Significant independent association between triglycerides and lecithin–cholesterol acyltransferase activity might indicate a role of hypertriglyceridaemia in alterations of lecithin–cholesterol acyltransferase activity in individuals with elevated fatty liver index.",
publisher = "Sage Publications Ltd",
journal = "Annals of Clinical Biochemistry",
title = "The association between lecithin–cholesterol acyltransferase activity and fatty liver index",
volume = "56",
number = "5",
pages = "583-592",
doi = "10.1177/0004563219853596"
}

Janać, J., Zeljković, A., Jelić-Ivanović, Z., Dimitrijević-Srećković, V., Miljković, M., Stefanović, A., Munjas, J., Vekić, J., Kotur-Stevuljević, J.,& Spasojević-Kalimanovska, V.. (2019). The association between lecithin–cholesterol acyltransferase activity and fatty liver index. in Annals of Clinical Biochemistry
Sage Publications Ltd., 56(5), 583-592.
https://doi.org/10.1177/0004563219853596

Janać J, Zeljković A, Jelić-Ivanović Z, Dimitrijević-Srećković V, Miljković M, Stefanović A, Munjas J, Vekić J, Kotur-Stevuljević J, Spasojević-Kalimanovska V. The association between lecithin–cholesterol acyltransferase activity and fatty liver index. in Annals of Clinical Biochemistry. 2019;56(5):583-592.
doi:10.1177/0004563219853596 .

Janać, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Dimitrijević-Srećković, Vesna, Miljković, Milica, Stefanović, Aleksandra, Munjas, Jelena, Vekić, Jelena, Kotur-Stevuljević, Jelena, Spasojević-Kalimanovska, Vesna, "The association between lecithin–cholesterol acyltransferase activity and fatty liver index" in Annals of Clinical Biochemistry, 56, no. 5 (2019):583-592,
https://doi.org/10.1177/0004563219853596 . .

TY  - JOUR
AU  - Mihajlović, Marija
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Kotur-Stevuljević, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Aleksandra
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3334
AB  - Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL-C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p  lt  .001) were observed in cohort of patients, alongside with decreased LCAT (p  lt  .050) and increased CETP activity (p  lt  .050). Both PON-1 activities were diminished in CRC (p  lt  .050 and p  lt  .001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p  lt  .001), CETP activity (OR = 1.010, p  lt  .01) and mass (OR = 0.994, p  lt  .001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p  lt  .001; OR = 0.982, p  lt  .050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer
VL  - 63
SP  - 32
EP  - 38
DO  - 10.1016/j.clinbiochem.2018.11.010
ER  - 

@article{
author = "Mihajlović, Marija and Gojković, Tamara and Vladimirov, Sandra and Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Zeljković, Dejan and Trifunović, Bratislav and Kotur-Stevuljević, Jelena and Spasojević-Kalimanovska, Vesna and Zeljković, Aleksandra",
year = "2019",
abstract = "Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL-C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p  lt  .001) were observed in cohort of patients, alongside with decreased LCAT (p  lt  .050) and increased CETP activity (p  lt  .050). Both PON-1 activities were diminished in CRC (p  lt  .050 and p  lt  .001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p  lt  .001), CETP activity (OR = 1.010, p  lt  .01) and mass (OR = 0.994, p  lt  .001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p  lt  .001; OR = 0.982, p  lt  .050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer",
volume = "63",
pages = "32-38",
doi = "10.1016/j.clinbiochem.2018.11.010"
}

Mihajlović, M., Gojković, T., Vladimirov, S., Miljković, M., Stefanović, A., Vekić, J., Zeljković, D., Trifunović, B., Kotur-Stevuljević, J., Spasojević-Kalimanovska, V.,& Zeljković, A.. (2019). Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 63, 32-38.
https://doi.org/10.1016/j.clinbiochem.2018.11.010

Mihajlović M, Gojković T, Vladimirov S, Miljković M, Stefanović A, Vekić J, Zeljković D, Trifunović B, Kotur-Stevuljević J, Spasojević-Kalimanovska V, Zeljković A. Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer. in Clinical Biochemistry. 2019;63:32-38.
doi:10.1016/j.clinbiochem.2018.11.010 .

Mihajlović, Marija, Gojković, Tamara, Vladimirov, Sandra, Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Zeljković, Dejan, Trifunović, Bratislav, Kotur-Stevuljević, Jelena, Spasojević-Kalimanovska, Vesna, Zeljković, Aleksandra, "Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer" in Clinical Biochemistry, 63 (2019):32-38,
https://doi.org/10.1016/j.clinbiochem.2018.11.010 . .

TY  - JOUR
AU  - Mihajlović, Marija
AU  - Ninić, Ana
AU  - Sopić, Miron
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Stjepanović, Željka
AU  - Zeljković, Aleksandra
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3458
AB  - Background: Elevated concentrations of resistin have been reported in colorectal cancer (CRC), but its interactions with adenylate cyclase-associated protein 1 (CAP-1) are largely unexplored. We investigated resistin plasma concentration, peripheral blood mononuclear cells (PBMCs) resistin messenger ribonucleic acid (mRNA), and CAP-1 mRNA levels in CRC patients, as well as the impact of resistin gene polymorphism rs1862513 on the examined markers. We also explored associations of resistin with high-density lipoprotein cholesterol (HDL-C) and predictive potential of our parameters for CRC. Methods: Eighty-six patients with CRC and 75 healthy adults were included. Commercial ELISA kit was used for obtaining resistin’s concentrations, while polymerase chain reaction (PCR) method was applied for evaluation of resistin and CAP-1 mRNA levels and rs1862513 polymorphism. Results: Plasma resistin and CAP-1 mRNA levels were higher in CRC patients (p < 0.001 and p < 0.05, respectively), while resistin mRNA levels were lower (p < 0.001). Negative association existed among plasma resistin and HDL-C concentrations (ρ = − 0.280; p < 0.05). A model including age, body-mass index, HDL-C, low-density lipoprotein cholesterol (LDL-C), and plasma resistin concentrations as independent predictors of CRC showed very good diagnostic accuracy (AUC = 0.898). We found no associations of rs1862513 with the examined markers. Conclusions: Our study demonstrated increased plasma resistin and CAP-1 mRNA levels, implying their possible interaction in CRC. The association among plasma resistin and HDL-C might indicate that HDL-C is involved in alterations of resistin’s secretion process. As a hallmark of personalized medicine, multi-marker approach in determination of resistin-related parameters might be useful for prediction and prevention of CRC development.
PB  - Springer
T2  - EPMA Journal
T1  - Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine
VL  - 10
SP  - 307
EP  - 316
DO  - 10.1007/s13167-019-00178-x
ER  - 

@article{
author = "Mihajlović, Marija and Ninić, Ana and Sopić, Miron and Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Zeljković, Dejan and Trifunović, Bratislav and Stjepanović, Željka and Zeljković, Aleksandra",
year = "2019",
abstract = "Background: Elevated concentrations of resistin have been reported in colorectal cancer (CRC), but its interactions with adenylate cyclase-associated protein 1 (CAP-1) are largely unexplored. We investigated resistin plasma concentration, peripheral blood mononuclear cells (PBMCs) resistin messenger ribonucleic acid (mRNA), and CAP-1 mRNA levels in CRC patients, as well as the impact of resistin gene polymorphism rs1862513 on the examined markers. We also explored associations of resistin with high-density lipoprotein cholesterol (HDL-C) and predictive potential of our parameters for CRC. Methods: Eighty-six patients with CRC and 75 healthy adults were included. Commercial ELISA kit was used for obtaining resistin’s concentrations, while polymerase chain reaction (PCR) method was applied for evaluation of resistin and CAP-1 mRNA levels and rs1862513 polymorphism. Results: Plasma resistin and CAP-1 mRNA levels were higher in CRC patients (p < 0.001 and p < 0.05, respectively), while resistin mRNA levels were lower (p < 0.001). Negative association existed among plasma resistin and HDL-C concentrations (ρ = − 0.280; p < 0.05). A model including age, body-mass index, HDL-C, low-density lipoprotein cholesterol (LDL-C), and plasma resistin concentrations as independent predictors of CRC showed very good diagnostic accuracy (AUC = 0.898). We found no associations of rs1862513 with the examined markers. Conclusions: Our study demonstrated increased plasma resistin and CAP-1 mRNA levels, implying their possible interaction in CRC. The association among plasma resistin and HDL-C might indicate that HDL-C is involved in alterations of resistin’s secretion process. As a hallmark of personalized medicine, multi-marker approach in determination of resistin-related parameters might be useful for prediction and prevention of CRC development.",
publisher = "Springer",
journal = "EPMA Journal",
title = "Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine",
volume = "10",
pages = "307-316",
doi = "10.1007/s13167-019-00178-x"
}

Mihajlović, M., Ninić, A., Sopić, M., Miljković, M., Stefanović, A., Vekić, J., Spasojević-Kalimanovska, V., Zeljković, D., Trifunović, B., Stjepanović, Ž.,& Zeljković, A.. (2019). Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine. in EPMA Journal
Springer., 10, 307-316.
https://doi.org/10.1007/s13167-019-00178-x

Mihajlović M, Ninić A, Sopić M, Miljković M, Stefanović A, Vekić J, Spasojević-Kalimanovska V, Zeljković D, Trifunović B, Stjepanović Ž, Zeljković A. Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine. in EPMA Journal. 2019;10:307-316.
doi:10.1007/s13167-019-00178-x .

Mihajlović, Marija, Ninić, Ana, Sopić, Miron, Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Zeljković, Dejan, Trifunović, Bratislav, Stjepanović, Željka, Zeljković, Aleksandra, "Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine" in EPMA Journal, 10 (2019):307-316,
https://doi.org/10.1007/s13167-019-00178-x . .

TY  - JOUR
AU  - Bojanin, Dragana
AU  - Vekić, Jelena
AU  - Milenković, Tatjana
AU  - Vuković, Rade
AU  - Zeljković, Aleksandra
AU  - Stefanović, Aleksandra
AU  - Janać, Jelena
AU  - Ivanišević, Jasmina
AU  - Mitrović, Katarina
AU  - Miljković, Milica
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3326
AB  - Background and aims: Dyslipidemia in type 1 diabetes mellitus (T1DM) is characterised by altered distributions of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses. Recent studies suggested that proprotein convertase subtilisin/kexin 9 (PCSK9) may contribute to the development of dyslipidemia in T1DM. In this cross-sectional study, we investigated the association between PCSK9 and lipoprotein subclasses in young T1DM patients, with respect to glycemic control. Methods: Plasma PCSK9 and lipoprotein subclasses were determined in 207 patients with T1DM (106 boys and 101 girls), aged 13.9 +/- 3.0 years and treated by intensive insulin therapy. Results: Plasma PCSK9 levels significantly increased with worsening of glycemic control (p  lt  0.001). T1DM patients with poor glucoregulation had the highest proportion of small, dense LDL (sdLDL) and smaller HDL particles, as well. PCSK9 was positively associated with markers of glucose homeostasis and serum lipid parameters only in patients with suboptimal/poor glucoregulation. In well-controlled T1DM, plasma PCSK9 level was inversely associated with a relative proportion of sdLDL particles (p  lt  0.01) and this association remained significant in multivariate analysis. In T1DM patients with suboptimal/poor glycemic control, PCSK9 was positively associated with the proportion of the smallest HDL3c particles (p  lt  0.001), but negatively with HDL size (p  lt  0.05). Conclusions: The extent of achieved metabolic control modifies the association between PCSK9 and lipoprotein subclasses in T1DM. Further investigations are needed to reveal whether the observed effects of glycemic control on PCSK9 and sdLDL levels have causal consequences on CVD risk in young patients with T1DM.
PB  - Elsevier Ireland Ltd, Clare
T2  - Atherosclerosis
T1  - Association between proprotein convertase subtilisin/kexin 9 (PCSK9) and lipoprotein subclasses in children with type 1 diabetes mellitus: Effects of glycemic control
VL  - 280
SP  - 14
EP  - 20
DO  - 10.1016/j.atherosclerosis.2018.11.020
ER  - 

@article{
author = "Bojanin, Dragana and Vekić, Jelena and Milenković, Tatjana and Vuković, Rade and Zeljković, Aleksandra and Stefanović, Aleksandra and Janać, Jelena and Ivanišević, Jasmina and Mitrović, Katarina and Miljković, Milica and Spasojević-Kalimanovska, Vesna",
year = "2019",
abstract = "Background and aims: Dyslipidemia in type 1 diabetes mellitus (T1DM) is characterised by altered distributions of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses. Recent studies suggested that proprotein convertase subtilisin/kexin 9 (PCSK9) may contribute to the development of dyslipidemia in T1DM. In this cross-sectional study, we investigated the association between PCSK9 and lipoprotein subclasses in young T1DM patients, with respect to glycemic control. Methods: Plasma PCSK9 and lipoprotein subclasses were determined in 207 patients with T1DM (106 boys and 101 girls), aged 13.9 +/- 3.0 years and treated by intensive insulin therapy. Results: Plasma PCSK9 levels significantly increased with worsening of glycemic control (p  lt  0.001). T1DM patients with poor glucoregulation had the highest proportion of small, dense LDL (sdLDL) and smaller HDL particles, as well. PCSK9 was positively associated with markers of glucose homeostasis and serum lipid parameters only in patients with suboptimal/poor glucoregulation. In well-controlled T1DM, plasma PCSK9 level was inversely associated with a relative proportion of sdLDL particles (p  lt  0.01) and this association remained significant in multivariate analysis. In T1DM patients with suboptimal/poor glycemic control, PCSK9 was positively associated with the proportion of the smallest HDL3c particles (p  lt  0.001), but negatively with HDL size (p  lt  0.05). Conclusions: The extent of achieved metabolic control modifies the association between PCSK9 and lipoprotein subclasses in T1DM. Further investigations are needed to reveal whether the observed effects of glycemic control on PCSK9 and sdLDL levels have causal consequences on CVD risk in young patients with T1DM.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Association between proprotein convertase subtilisin/kexin 9 (PCSK9) and lipoprotein subclasses in children with type 1 diabetes mellitus: Effects of glycemic control",
volume = "280",
pages = "14-20",
doi = "10.1016/j.atherosclerosis.2018.11.020"
}

Bojanin, D., Vekić, J., Milenković, T., Vuković, R., Zeljković, A., Stefanović, A., Janać, J., Ivanišević, J., Mitrović, K., Miljković, M.,& Spasojević-Kalimanovska, V.. (2019). Association between proprotein convertase subtilisin/kexin 9 (PCSK9) and lipoprotein subclasses in children with type 1 diabetes mellitus: Effects of glycemic control. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 280, 14-20.
https://doi.org/10.1016/j.atherosclerosis.2018.11.020

Bojanin D, Vekić J, Milenković T, Vuković R, Zeljković A, Stefanović A, Janać J, Ivanišević J, Mitrović K, Miljković M, Spasojević-Kalimanovska V. Association between proprotein convertase subtilisin/kexin 9 (PCSK9) and lipoprotein subclasses in children with type 1 diabetes mellitus: Effects of glycemic control. in Atherosclerosis. 2019;280:14-20.
doi:10.1016/j.atherosclerosis.2018.11.020 .

Bojanin, Dragana, Vekić, Jelena, Milenković, Tatjana, Vuković, Rade, Zeljković, Aleksandra, Stefanović, Aleksandra, Janać, Jelena, Ivanišević, Jasmina, Mitrović, Katarina, Miljković, Milica, Spasojević-Kalimanovska, Vesna, "Association between proprotein convertase subtilisin/kexin 9 (PCSK9) and lipoprotein subclasses in children with type 1 diabetes mellitus: Effects of glycemic control" in Atherosclerosis, 280 (2019):14-20,
https://doi.org/10.1016/j.atherosclerosis.2018.11.020 . .

TY  - THES
AU  - Miljković, Milica
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6253
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18879/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=2048320098
UR  - http://nardus.mpn.gov.rs/123456789/10295
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3400
AB  - Hronična  bolest  bubrega  (HBB)  predstavlja  stanje  koje  se  karakteriše  brojnim tradicionalnim i netradicionalnim faktorima rizika za razvoj kardiovaskularnih bolesti (KVB). Dislipidemija,  oksidativni  stres  i  inflamacija  zahvaljujući  međusobnim  interakcijama predstavljaju  faktore  koji  značajno  doprinose  patogenezi  i  progresiji  ateroskleroze  kod bolesnika  sa  HBB.  Paraoksonaza  1  (PON1)  je  glavni  antioksidativni  enzim  na  HDL česticama,  koji  svoj  antiaterogeni  potencijal  ostvaruje  mehanizmima  koji  sprečavaju oksidaciju lipoproteina niske gustine (LDL). Prema in vitro studijama u reverznom transportu holesterola mogu učestvovati i membrane eritrocita koje razmenjuju holesterol (RBC-Mh) sa drugim  lipoproteinima, kao  i hemoglobin koji privremeno uklanja višak holesterola  tako što gradi Hb-holesterol kompleks  (Hb-h). Brojne  studije  su pokazale da  inflamacija  ima važnu ulogu  u  inicijaciji  i  progresiji  ateroskleroze.  Takođe,  remodelovanje  ekstracelularnog matriksa (ECM) i neadekvatna aktivnost matriks metaloproteinaza (MMP) i tkivnih inhibitora matriks metaloproteinaza  (TIMP), kao  i galektin-3 doprinose napredovanju hronične bolesti bubrega  i  razvoju  ateroskleroze  kod  ovih  bolesnika.    Cilj  ove  studije  je  bio  ispitivanje parametara  dislipidemije,  oksidativnog  stresa  i  inflamacije  u  krvi  bolesnika  sa  HBB, bolesnika  na  hemodijalizi    i  zdravih  ispitanika.  Takođe,  ova  studija  je  imala  za  cilj  i ispitivanje  uticaja  pomenutih  faktora  rizika  na  redoks  status  u  glavnim  lipoproteinskim frakcijama  i  na  remodelovanje  ECM.  Cilj  je  bio  i  izračunati  odgovarajuće  skorove dislipidemije, oksidativnog stresa  i  inflamacije kako bi se procenio sinergistički efekat ovih procesa  kao  i  efekat  koji  zajedno  ostvaruju  na  oksidativno-stresni  status  u  lipoproteinskim frakcijama kod bolesnika sa različitim stepenom bubrežnog oštećenja. U  istraživanju  je učestvovalo 77 bolesnika sa  različitim stepenom oštećenja  funkcije bubrega  i  40  zdravih  ispitanika.  Istraživanje  je  planirano  i  sprovedeno  prema  etičkim principima u skladu sa Helsinškom deklaracijom. Svi  parametri  oksidativnog  stresa,  totalni  oksidativni  status  (p<0,01),  uznapredovali produkti  oksidacije  proteina  (AOPP)  (p<0,01)  i  tiobarbiturna  kiselina  reagujuće  supstance (p<0,05)  su bili  značajno viši kod bolesnika  sa oštećenom  funkcijom bubrega u odnosu na zdrave ispitanike. Vrednosti  Hb-h u obe grupe bolesnika su bile niže u odnosu na kontrolnu grupu (p<0,001), dok je s druge strane RBC-Mh bio povišen kod bolesnika na hemodijalizi u poređenju sa kontrolnom grupom (p<0,01). Zdravi  ispitanici su  imali značajno viši relativni udeo HDL2 subfrakcija u odnosu na bubrežne bolesnike (p<0,05).  Koncentracija i aktivnost PON1 u serumu su bile značajno niže u obe grupe bolesnika u odnosu na zdrave  ispitanike (p<0,001)...
AB  - Chronic kidney disease (CKD) presents condition that is characterized by numeroustraditional and non-traditional risk factors for developing cardiovascular disease (CVD).Dyslipidemia, oxidative stress and inflammation due to mutual interaction are factors thatsignificantly contribute to the pathogenesis and progression of atherosclerosis in patients withCKD. Paraoxonase 1 (PON1) is the main antioxidant enzyme on HDL particles, which hasantiatherogenic potential by mechanisms that inhibit oxidation of low density lipoproteinparticles (LDL). In accordance to in vitro studies reverse cholesterol transport could involveerythrocyte membranes that exchange free cholesterol (RBC-Mc) with other lipoproteins, aswell as hemoglobin that temporarily removes excess of cholesterol by building Hbcholesterolcomplex (Hb-c). Numerous studies have shown that inflammation plays animportant role in initiation and progression of atherosclerosis. Also, extracellular matrix(ECM) remodeling and inadequate activity of matrix metalloproteinase (MMP) and tissuematrix metalloproteinase inhibitors (TIMP) as well as galectin-3 also contribute to theprogression of chronic kidney disease and atherosclerosis in these patients. The aim of thisstudy was to examine the parameters of dyslipidemia, oxidative stress and inflammation inserum of patients with CKD, dialysis patients and healthy subjects. Also, this study wasaimed at examining the influence of the mentioned risk factors on redox status in majorlipoprotein fractions and on ECM remodeling. The aim was to calculate summary score ofdyslipidemia, oxidative stress and inflammation to evaluate the synergistic effect of theseprocesses as well as the effect on oxidative stress status in lipoprotein fractions in patientswith different degrees of renal impairment.The study included 77 patients with different stage of renal function impairment and40 healthy subjects. The research is planned and implemented according to ethical principlesin accordance with the Helsinki Declaration.Healthy subjects had a significantly higher percentage of HDL2 subfractionscompared to renal patients (p <0.05). The values of Hb-c in both patients groups were lowerthan in control group (p <0.001), while values of RBC-Mc were elevated in dialysis patientscompared to the control group (p <0.01). All parameters of oxidative stress, total oxidativestatus (p <0.01), advanced oxidation protein products (AOPP) (p <0.01) and thiobarbituricacid reactive substances (p <0.05) were significantly higher in patients with impaired renalfunction compared to healthy subjects. Concentration and activity of PON1 in serum weresignificantly lower in both groups of patients compared to healthy subjects (p <0.001).Arylesterase activity of PON1 was significantly lower in renal patients on HDL2 and HDL3subclasses (p <0.001). It was found that after hemodialysis there was a significant increase inparaoxonase activity of PON1 (p <0.01), while arylesterase activity of PON1 was lower afterdialysis (p <0.01)...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Oksidativno-stresni status u krvi i lipoproteinskim frakcijama pacijenata sa hroničnom bolešću bubrega
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10295
ER  - 

@phdthesis{
author = "Miljković, Milica",
year = "2018",
abstract = "Hronična  bolest  bubrega  (HBB)  predstavlja  stanje  koje  se  karakteriše  brojnim tradicionalnim i netradicionalnim faktorima rizika za razvoj kardiovaskularnih bolesti (KVB). Dislipidemija,  oksidativni  stres  i  inflamacija  zahvaljujući  međusobnim  interakcijama predstavljaju  faktore  koji  značajno  doprinose  patogenezi  i  progresiji  ateroskleroze  kod bolesnika  sa  HBB.  Paraoksonaza  1  (PON1)  je  glavni  antioksidativni  enzim  na  HDL česticama,  koji  svoj  antiaterogeni  potencijal  ostvaruje  mehanizmima  koji  sprečavaju oksidaciju lipoproteina niske gustine (LDL). Prema in vitro studijama u reverznom transportu holesterola mogu učestvovati i membrane eritrocita koje razmenjuju holesterol (RBC-Mh) sa drugim  lipoproteinima, kao  i hemoglobin koji privremeno uklanja višak holesterola  tako što gradi Hb-holesterol kompleks  (Hb-h). Brojne  studije  su pokazale da  inflamacija  ima važnu ulogu  u  inicijaciji  i  progresiji  ateroskleroze.  Takođe,  remodelovanje  ekstracelularnog matriksa (ECM) i neadekvatna aktivnost matriks metaloproteinaza (MMP) i tkivnih inhibitora matriks metaloproteinaza  (TIMP), kao  i galektin-3 doprinose napredovanju hronične bolesti bubrega  i  razvoju  ateroskleroze  kod  ovih  bolesnika.    Cilj  ove  studije  je  bio  ispitivanje parametara  dislipidemije,  oksidativnog  stresa  i  inflamacije  u  krvi  bolesnika  sa  HBB, bolesnika  na  hemodijalizi    i  zdravih  ispitanika.  Takođe,  ova  studija  je  imala  za  cilj  i ispitivanje  uticaja  pomenutih  faktora  rizika  na  redoks  status  u  glavnim  lipoproteinskim frakcijama  i  na  remodelovanje  ECM.  Cilj  je  bio  i  izračunati  odgovarajuće  skorove dislipidemije, oksidativnog stresa  i  inflamacije kako bi se procenio sinergistički efekat ovih procesa  kao  i  efekat  koji  zajedno  ostvaruju  na  oksidativno-stresni  status  u  lipoproteinskim frakcijama kod bolesnika sa različitim stepenom bubrežnog oštećenja. U  istraživanju  je učestvovalo 77 bolesnika sa  različitim stepenom oštećenja  funkcije bubrega  i  40  zdravih  ispitanika.  Istraživanje  je  planirano  i  sprovedeno  prema  etičkim principima u skladu sa Helsinškom deklaracijom. Svi  parametri  oksidativnog  stresa,  totalni  oksidativni  status  (p<0,01),  uznapredovali produkti  oksidacije  proteina  (AOPP)  (p<0,01)  i  tiobarbiturna  kiselina  reagujuće  supstance (p<0,05)  su bili  značajno viši kod bolesnika  sa oštećenom  funkcijom bubrega u odnosu na zdrave ispitanike. Vrednosti  Hb-h u obe grupe bolesnika su bile niže u odnosu na kontrolnu grupu (p<0,001), dok je s druge strane RBC-Mh bio povišen kod bolesnika na hemodijalizi u poređenju sa kontrolnom grupom (p<0,01). Zdravi  ispitanici su  imali značajno viši relativni udeo HDL2 subfrakcija u odnosu na bubrežne bolesnike (p<0,05).  Koncentracija i aktivnost PON1 u serumu su bile značajno niže u obe grupe bolesnika u odnosu na zdrave  ispitanike (p<0,001)..., Chronic kidney disease (CKD) presents condition that is characterized by numeroustraditional and non-traditional risk factors for developing cardiovascular disease (CVD).Dyslipidemia, oxidative stress and inflammation due to mutual interaction are factors thatsignificantly contribute to the pathogenesis and progression of atherosclerosis in patients withCKD. Paraoxonase 1 (PON1) is the main antioxidant enzyme on HDL particles, which hasantiatherogenic potential by mechanisms that inhibit oxidation of low density lipoproteinparticles (LDL). In accordance to in vitro studies reverse cholesterol transport could involveerythrocyte membranes that exchange free cholesterol (RBC-Mc) with other lipoproteins, aswell as hemoglobin that temporarily removes excess of cholesterol by building Hbcholesterolcomplex (Hb-c). Numerous studies have shown that inflammation plays animportant role in initiation and progression of atherosclerosis. Also, extracellular matrix(ECM) remodeling and inadequate activity of matrix metalloproteinase (MMP) and tissuematrix metalloproteinase inhibitors (TIMP) as well as galectin-3 also contribute to theprogression of chronic kidney disease and atherosclerosis in these patients. The aim of thisstudy was to examine the parameters of dyslipidemia, oxidative stress and inflammation inserum of patients with CKD, dialysis patients and healthy subjects. Also, this study wasaimed at examining the influence of the mentioned risk factors on redox status in majorlipoprotein fractions and on ECM remodeling. The aim was to calculate summary score ofdyslipidemia, oxidative stress and inflammation to evaluate the synergistic effect of theseprocesses as well as the effect on oxidative stress status in lipoprotein fractions in patientswith different degrees of renal impairment.The study included 77 patients with different stage of renal function impairment and40 healthy subjects. The research is planned and implemented according to ethical principlesin accordance with the Helsinki Declaration.Healthy subjects had a significantly higher percentage of HDL2 subfractionscompared to renal patients (p <0.05). The values of Hb-c in both patients groups were lowerthan in control group (p <0.001), while values of RBC-Mc were elevated in dialysis patientscompared to the control group (p <0.01). All parameters of oxidative stress, total oxidativestatus (p <0.01), advanced oxidation protein products (AOPP) (p <0.01) and thiobarbituricacid reactive substances (p <0.05) were significantly higher in patients with impaired renalfunction compared to healthy subjects. Concentration and activity of PON1 in serum weresignificantly lower in both groups of patients compared to healthy subjects (p <0.001).Arylesterase activity of PON1 was significantly lower in renal patients on HDL2 and HDL3subclasses (p <0.001). It was found that after hemodialysis there was a significant increase inparaoxonase activity of PON1 (p <0.01), while arylesterase activity of PON1 was lower afterdialysis (p <0.01)...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Oksidativno-stresni status u krvi i lipoproteinskim frakcijama pacijenata sa hroničnom bolešću bubrega",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10295"
}

Miljković, M.. (2018). Oksidativno-stresni status u krvi i lipoproteinskim frakcijama pacijenata sa hroničnom bolešću bubrega. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_10295

Miljković M. Oksidativno-stresni status u krvi i lipoproteinskim frakcijama pacijenata sa hroničnom bolešću bubrega. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10295 .

Miljković, Milica, "Oksidativno-stresni status u krvi i lipoproteinskim frakcijama pacijenata sa hroničnom bolešću bubrega" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10295 .

TY  - CONF
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Kalimanovska-Oštrić, Dimitra
AU  - Đuričić, Ivana
AU  - Jelić-Ivanović, Zorana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3071
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis
T1  - Association of statin therapy with plasma fatty acids profile and lipoprotein-associated phospholipase A2 concentration
VL  - 275
SP  - e60
EP  - e60
DO  - 10.1016/j.atherosclerosis.2018.06.164
ER  - 

@conference{
author = "Gojković, Tamara and Vladimirov, Sandra and Spasojević-Kalimanovska, Vesna and Zeljković, Aleksandra and Vekić, Jelena and Miljković, Milica and Stefanović, Aleksandra and Kalimanovska-Oštrić, Dimitra and Đuričić, Ivana and Jelić-Ivanović, Zorana",
year = "2018",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Association of statin therapy with plasma fatty acids profile and lipoprotein-associated phospholipase A2 concentration",
volume = "275",
pages = "e60-e60",
doi = "10.1016/j.atherosclerosis.2018.06.164"
}

Gojković, T., Vladimirov, S., Spasojević-Kalimanovska, V., Zeljković, A., Vekić, J., Miljković, M., Stefanović, A., Kalimanovska-Oštrić, D., Đuričić, I.,& Jelić-Ivanović, Z.. (2018). Association of statin therapy with plasma fatty acids profile and lipoprotein-associated phospholipase A2 concentration. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 275, e60-e60.
https://doi.org/10.1016/j.atherosclerosis.2018.06.164

Gojković T, Vladimirov S, Spasojević-Kalimanovska V, Zeljković A, Vekić J, Miljković M, Stefanović A, Kalimanovska-Oštrić D, Đuričić I, Jelić-Ivanović Z. Association of statin therapy with plasma fatty acids profile and lipoprotein-associated phospholipase A2 concentration. in Atherosclerosis. 2018;275:e60-e60.
doi:10.1016/j.atherosclerosis.2018.06.164 .

Gojković, Tamara, Vladimirov, Sandra, Spasojević-Kalimanovska, Vesna, Zeljković, Aleksandra, Vekić, Jelena, Miljković, Milica, Stefanović, Aleksandra, Kalimanovska-Oštrić, Dimitra, Đuričić, Ivana, Jelić-Ivanović, Zorana, "Association of statin therapy with plasma fatty acids profile and lipoprotein-associated phospholipase A2 concentration" in Atherosclerosis, 275 (2018):e60-e60,
https://doi.org/10.1016/j.atherosclerosis.2018.06.164 . .

TY  - CONF
AU  - Vladimirov, Sandra
AU  - Gojković, Tamara
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Miljković, Milica
AU  - Bogavac-Stanojević, Nataša
AU  - Stepanović, Željka
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3073
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis
T1  - Association of cholesterol homeostasis parameters with cardiovascular risk factors in healthy population
VL  - 275
SP  - e86
EP  - e86
DO  - 10.1016/j.atherosclerosis.2018.06.238
ER  - 

@conference{
author = "Vladimirov, Sandra and Gojković, Tamara and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Miljković, Milica and Bogavac-Stanojević, Nataša and Stepanović, Željka and Spasojević-Kalimanovska, Vesna",
year = "2018",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Association of cholesterol homeostasis parameters with cardiovascular risk factors in healthy population",
volume = "275",
pages = "e86-e86",
doi = "10.1016/j.atherosclerosis.2018.06.238"
}

Vladimirov, S., Gojković, T., Zeljković, A., Jelić-Ivanović, Z., Miljković, M., Bogavac-Stanojević, N., Stepanović, Ž.,& Spasojević-Kalimanovska, V.. (2018). Association of cholesterol homeostasis parameters with cardiovascular risk factors in healthy population. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 275, e86-e86.
https://doi.org/10.1016/j.atherosclerosis.2018.06.238

Vladimirov S, Gojković T, Zeljković A, Jelić-Ivanović Z, Miljković M, Bogavac-Stanojević N, Stepanović Ž, Spasojević-Kalimanovska V. Association of cholesterol homeostasis parameters with cardiovascular risk factors in healthy population. in Atherosclerosis. 2018;275:e86-e86.
doi:10.1016/j.atherosclerosis.2018.06.238 .

Vladimirov, Sandra, Gojković, Tamara, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Miljković, Milica, Bogavac-Stanojević, Nataša, Stepanović, Željka, Spasojević-Kalimanovska, Vesna, "Association of cholesterol homeostasis parameters with cardiovascular risk factors in healthy population" in Atherosclerosis, 275 (2018):e86-e86,
https://doi.org/10.1016/j.atherosclerosis.2018.06.238 . .

TY  - JOUR
AU  - Michalickova, Danica
AU  - Kotur-Stevuljević, Jelena
AU  - Miljković, Milica
AU  - Dikić, Nenad
AU  - Kostić-Vučićević, Marija
AU  - Anđelković, Marija
AU  - Koricanac, Vladimir
AU  - Đorđević, Brižita
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3201
AB  - A randomized, double-blind, placebo-controlled study was conducted, in order to evaluate if Lactobacillus helveticus Lafti (R) L10 (Lallemand Health Solutions, Montreal, Canada) supplementation during three months could influence oxidative markers in the population of elite athletes: triathletes, cyclists and endurance athletes. Twenty-two elite athletes were randomized to either placebo (n = 12) or probiotic (n = 10) groups. The probiotic group received 2x1010 colony forming units of Lafti (R) L10. Before and after the supplementation serum samples were collected. Markers of oxidative stress and anti-oxidative defense: superoxide dismutase (SOD), paraoxonase (PON), advanced oxidation protein products (AOPP), malondialdehyde (MDA), total antioxidant status, total oxidant status, prooxidant-antioxidant balance, oxidative stress index, bilirubin, uric acid and albumin were determined in serum. Parameters of lipid status, as well as susceptibility to copper-induced oxidation of LDL particles in vitro were also determined. There was a significant interaction effect for MDA (p = 0.039), with a decrease in MDA in the probiotic group only (p = 0.049). There was a significant interaction effect for AOPP (p = 0.037), with a significant decrease in the probiotic group (p = 0.045). Interaction effect for SOD was approaching to formal significance (p = 0.108) and the post-hoc test showed a significant decrease in the probiotic group (p = 0.041) only. A significant correlation between AOPP and SOD (p = 0.012, r = -0.40) was found in the probiotic group at the end of the study. PON1 activity was decreased in both the probiotic (p = 0.032) and placebo group (p = 0.035). No significant changes in the remainder of the evaluated parameters were noted. In conclusion, probiotic strain Lafti (R) L10 exerts certain antioxidant potential, but further research is needed.
PB  - De Gruyter Poland Sp Zoo, Warsaw
T2  - Journal of Human Kinetics
T1  - Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study
VL  - 64
IS  - 1
SP  - 111
EP  - 122
DO  - 10.1515/hukin-2017-0203
ER  - 

@article{
author = "Michalickova, Danica and Kotur-Stevuljević, Jelena and Miljković, Milica and Dikić, Nenad and Kostić-Vučićević, Marija and Anđelković, Marija and Koricanac, Vladimir and Đorđević, Brižita",
year = "2018",
abstract = "A randomized, double-blind, placebo-controlled study was conducted, in order to evaluate if Lactobacillus helveticus Lafti (R) L10 (Lallemand Health Solutions, Montreal, Canada) supplementation during three months could influence oxidative markers in the population of elite athletes: triathletes, cyclists and endurance athletes. Twenty-two elite athletes were randomized to either placebo (n = 12) or probiotic (n = 10) groups. The probiotic group received 2x1010 colony forming units of Lafti (R) L10. Before and after the supplementation serum samples were collected. Markers of oxidative stress and anti-oxidative defense: superoxide dismutase (SOD), paraoxonase (PON), advanced oxidation protein products (AOPP), malondialdehyde (MDA), total antioxidant status, total oxidant status, prooxidant-antioxidant balance, oxidative stress index, bilirubin, uric acid and albumin were determined in serum. Parameters of lipid status, as well as susceptibility to copper-induced oxidation of LDL particles in vitro were also determined. There was a significant interaction effect for MDA (p = 0.039), with a decrease in MDA in the probiotic group only (p = 0.049). There was a significant interaction effect for AOPP (p = 0.037), with a significant decrease in the probiotic group (p = 0.045). Interaction effect for SOD was approaching to formal significance (p = 0.108) and the post-hoc test showed a significant decrease in the probiotic group (p = 0.041) only. A significant correlation between AOPP and SOD (p = 0.012, r = -0.40) was found in the probiotic group at the end of the study. PON1 activity was decreased in both the probiotic (p = 0.032) and placebo group (p = 0.035). No significant changes in the remainder of the evaluated parameters were noted. In conclusion, probiotic strain Lafti (R) L10 exerts certain antioxidant potential, but further research is needed.",
publisher = "De Gruyter Poland Sp Zoo, Warsaw",
journal = "Journal of Human Kinetics",
title = "Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study",
volume = "64",
number = "1",
pages = "111-122",
doi = "10.1515/hukin-2017-0203"
}

Michalickova, D., Kotur-Stevuljević, J., Miljković, M., Dikić, N., Kostić-Vučićević, M., Anđelković, M., Koricanac, V.,& Đorđević, B.. (2018). Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study. in Journal of Human Kinetics
De Gruyter Poland Sp Zoo, Warsaw., 64(1), 111-122.
https://doi.org/10.1515/hukin-2017-0203

Michalickova D, Kotur-Stevuljević J, Miljković M, Dikić N, Kostić-Vučićević M, Anđelković M, Koricanac V, Đorđević B. Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study. in Journal of Human Kinetics. 2018;64(1):111-122.
doi:10.1515/hukin-2017-0203 .

Michalickova, Danica, Kotur-Stevuljević, Jelena, Miljković, Milica, Dikić, Nenad, Kostić-Vučićević, Marija, Anđelković, Marija, Koricanac, Vladimir, Đorđević, Brižita, "Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study" in Journal of Human Kinetics, 64, no. 1 (2018):111-122,
https://doi.org/10.1515/hukin-2017-0203 . .

TY  - JOUR
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Simić-Ogrizović, Sanja
AU  - Vekić, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Cerne, Darko
AU  - Kocbek, Petra
AU  - Marc, Janja
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3085
AB  - Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant-antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score (P  lt  .001), oxy score (P  lt  .01), inflammation score (P  lt  .001), and the DOI score (P  lt  .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP (P  lt  .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction (P  lt  .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (rho = 0.510, P  lt  .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Angiology
T1  - Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease
VL  - 69
IS  - 10
SP  - 861
EP  - 870
DO  - 10.1177/0003319718780041
ER  - 

@article{
author = "Miljković, Milica and Stefanović, Aleksandra and Simić-Ogrizović, Sanja and Vekić, Jelena and Bogavac-Stanojević, Nataša and Cerne, Darko and Kocbek, Petra and Marc, Janja and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena",
year = "2018",
abstract = "Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant-antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score (P  lt  .001), oxy score (P  lt  .01), inflammation score (P  lt  .001), and the DOI score (P  lt  .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP (P  lt  .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction (P  lt  .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (rho = 0.510, P  lt  .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Angiology",
title = "Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease",
volume = "69",
number = "10",
pages = "861-870",
doi = "10.1177/0003319718780041"
}

Miljković, M., Stefanović, A., Simić-Ogrizović, S., Vekić, J., Bogavac-Stanojević, N., Cerne, D., Kocbek, P., Marc, J., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V.,& Kotur-Stevuljević, J.. (2018). Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease. in Angiology
Sage Publications Inc, Thousand Oaks., 69(10), 861-870.
https://doi.org/10.1177/0003319718780041

Miljković M, Stefanović A, Simić-Ogrizović S, Vekić J, Bogavac-Stanojević N, Cerne D, Kocbek P, Marc J, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Kotur-Stevuljević J. Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease. in Angiology. 2018;69(10):861-870.
doi:10.1177/0003319718780041 .

Miljković, Milica, Stefanović, Aleksandra, Simić-Ogrizović, Sanja, Vekić, Jelena, Bogavac-Stanojević, Nataša, Cerne, Darko, Kocbek, Petra, Marc, Janja, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, "Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease" in Angiology, 69, no. 10 (2018):861-870,
https://doi.org/10.1177/0003319718780041 . .

TY  - JOUR
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Gojković, Tamara
AU  - Simić-Ogrizović, Sanja
AU  - Bogavac-Stanojević, Nataša
AU  - Cerne, Darko
AU  - Ilić, Jasmina
AU  - Stefanović, Ivan
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3067
AB  - Introduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p  lt  0.01) and arylesterase activity (p  lt  0.001) of PON1 as well as its concentration (p  lt  0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3 subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p  lt  0.05) and ESRD (p  lt  0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p  lt  0.001) and HDL3 (CKD p  lt  0.05; ESRD patients p  lt  0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p  lt  0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease
VL  - 60
SP  - 52
EP  - 58
DO  - 10.1016/j.clinbiochem.2018.08.006
ER  - 

@article{
author = "Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Zeljković, Aleksandra and Gojković, Tamara and Simić-Ogrizović, Sanja and Bogavac-Stanojević, Nataša and Cerne, Darko and Ilić, Jasmina and Stefanović, Ivan and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena",
year = "2018",
abstract = "Introduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p  lt  0.01) and arylesterase activity (p  lt  0.001) of PON1 as well as its concentration (p  lt  0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3 subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p  lt  0.05) and ESRD (p  lt  0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p  lt  0.001) and HDL3 (CKD p  lt  0.05; ESRD patients p  lt  0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p  lt  0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease",
volume = "60",
pages = "52-58",
doi = "10.1016/j.clinbiochem.2018.08.006"
}

Miljković, M., Stefanović, A., Vekić, J., Zeljković, A., Gojković, T., Simić-Ogrizović, S., Bogavac-Stanojević, N., Cerne, D., Ilić, J., Stefanović, I., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V.,& Kotur-Stevuljević, J.. (2018). Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 60, 52-58.
https://doi.org/10.1016/j.clinbiochem.2018.08.006

Miljković M, Stefanović A, Vekić J, Zeljković A, Gojković T, Simić-Ogrizović S, Bogavac-Stanojević N, Cerne D, Ilić J, Stefanović I, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Kotur-Stevuljević J. Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease. in Clinical Biochemistry. 2018;60:52-58.
doi:10.1016/j.clinbiochem.2018.08.006 .

Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Zeljković, Aleksandra, Gojković, Tamara, Simić-Ogrizović, Sanja, Bogavac-Stanojević, Nataša, Cerne, Darko, Ilić, Jasmina, Stefanović, Ivan, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, "Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease" in Clinical Biochemistry, 60 (2018):52-58,
https://doi.org/10.1016/j.clinbiochem.2018.08.006 . .

TY  - JOUR
AU  - Miletić, Jadranka
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Petković, Marijana
AU  - Ilić, Tihomir V.
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Prostran, Milica
AU  - Stojanov, Marina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3163
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3420
AB  - Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
VL  - 128
IS  - 7
SP  - 600
EP  - 607
DO  - 10.1080/00207454.2017.1403916
ER  - 

@article{
author = "Miletić, Jadranka and Drakulić, Dunja and Pejić, Snežana and Petković, Marijana and Ilić, Tihomir V. and Miljković, Milica and Stefanović, Aleksandra and Prostran, Milica and Stojanov, Marina",
year = "2018",
abstract = "Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease",
volume = "128",
number = "7",
pages = "600-607",
doi = "10.1080/00207454.2017.1403916"
}

Miletić, J., Drakulić, D., Pejić, S., Petković, M., Ilić, T. V., Miljković, M., Stefanović, A., Prostran, M.,& Stojanov, M.. (2018). Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 128(7), 600-607.
https://doi.org/10.1080/00207454.2017.1403916

Miletić J, Drakulić D, Pejić S, Petković M, Ilić TV, Miljković M, Stefanović A, Prostran M, Stojanov M. Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience. 2018;128(7):600-607.
doi:10.1080/00207454.2017.1403916 .

Miletić, Jadranka, Drakulić, Dunja, Pejić, Snežana, Petković, Marijana, Ilić, Tihomir V., Miljković, Milica, Stefanović, Aleksandra, Prostran, Milica, Stojanov, Marina, "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease" in International Journal of Neuroscience, 128, no. 7 (2018):600-607,
https://doi.org/10.1080/00207454.2017.1403916 . .

TY  - JOUR
AU  - Miletić, Jadranka
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Petković, Marijana
AU  - Ilić, Tihomir V.
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Prostran, Milica
AU  - Stojanov, Marina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3163
AB  - Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
VL  - 128
IS  - 7
SP  - 600
EP  - 607
DO  - 10.1080/00207454.2017.1403916
ER  - 

@article{
author = "Miletić, Jadranka and Drakulić, Dunja and Pejić, Snežana and Petković, Marijana and Ilić, Tihomir V. and Miljković, Milica and Stefanović, Aleksandra and Prostran, Milica and Stojanov, Marina",
year = "2018",
abstract = "Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease",
volume = "128",
number = "7",
pages = "600-607",
doi = "10.1080/00207454.2017.1403916"
}

Miletić, J., Drakulić, D., Pejić, S., Petković, M., Ilić, T. V., Miljković, M., Stefanović, A., Prostran, M.,& Stojanov, M.. (2018). Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 128(7), 600-607.
https://doi.org/10.1080/00207454.2017.1403916

Miletić J, Drakulić D, Pejić S, Petković M, Ilić TV, Miljković M, Stefanović A, Prostran M, Stojanov M. Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience. 2018;128(7):600-607.
doi:10.1080/00207454.2017.1403916 .

Miletić, Jadranka, Drakulić, Dunja, Pejić, Snežana, Petković, Marijana, Ilić, Tihomir V., Miljković, Milica, Stefanović, Aleksandra, Prostran, Milica, Stojanov, Marina, "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease" in International Journal of Neuroscience, 128, no. 7 (2018):600-607,
https://doi.org/10.1080/00207454.2017.1403916 . .