TY  - JOUR
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Grubišić, Dragoljub
AU  - Petrović, Silvana
AU  - Kukić, Jelena
AU  - Ristić, Mihailo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1054
AB  - The chemical composition and antimicrobial and antiradical activities of the essential oils isolated from unripe and ripe fruits of S. globiferum Vis. (Apiaceae) have been determined. The most abundant constituent in the essential oils of unripe and ripe fruits was sabinene (53.1% and 65.3%), followed by gamma-terpinene (7.7% and 6.6%), alpha-pinene (7.2% and 4.4%), and beta-phellandrene (5.0% and 4.9%). Antibacterial and antifungal properties of these oils were evaluated using a modified microdilution technique. Scavenging activity was determined by the DPPH radical assay. The essential oils exhibited significant antimicrobial, but low antiradical activity.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Chemical Composition, Antimicrobial and Antiradical Properties of the Essential Oils of Seseli globiferum Fruits
VL  - 3
IS  - 11
SP  - 1935
EP  - 1938
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1054
ER  - 

@article{
author = "Stojković, Dejan and Glamočlija, Jasmina and Soković, Marina and Grubišić, Dragoljub and Petrović, Silvana and Kukić, Jelena and Ristić, Mihailo",
year = "2008",
abstract = "The chemical composition and antimicrobial and antiradical activities of the essential oils isolated from unripe and ripe fruits of S. globiferum Vis. (Apiaceae) have been determined. The most abundant constituent in the essential oils of unripe and ripe fruits was sabinene (53.1% and 65.3%), followed by gamma-terpinene (7.7% and 6.6%), alpha-pinene (7.2% and 4.4%), and beta-phellandrene (5.0% and 4.9%). Antibacterial and antifungal properties of these oils were evaluated using a modified microdilution technique. Scavenging activity was determined by the DPPH radical assay. The essential oils exhibited significant antimicrobial, but low antiradical activity.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Chemical Composition, Antimicrobial and Antiradical Properties of the Essential Oils of Seseli globiferum Fruits",
volume = "3",
number = "11",
pages = "1935-1938",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1054"
}

Stojković, D., Glamočlija, J., Soković, M., Grubišić, D., Petrović, S., Kukić, J.,& Ristić, M.. (2008). Chemical Composition, Antimicrobial and Antiradical Properties of the Essential Oils of Seseli globiferum Fruits. in Natural Product Communications
Natural Products Inc, Westerville., 3(11), 1935-1938.
https://hdl.handle.net/21.15107/rcub_farfar_1054

Stojković D, Glamočlija J, Soković M, Grubišić D, Petrović S, Kukić J, Ristić M. Chemical Composition, Antimicrobial and Antiradical Properties of the Essential Oils of Seseli globiferum Fruits. in Natural Product Communications. 2008;3(11):1935-1938.
https://hdl.handle.net/21.15107/rcub_farfar_1054 .

Stojković, Dejan, Glamočlija, Jasmina, Soković, Marina, Grubišić, Dragoljub, Petrović, Silvana, Kukić, Jelena, Ristić, Mihailo, "Chemical Composition, Antimicrobial and Antiradical Properties of the Essential Oils of Seseli globiferum Fruits" in Natural Product Communications, 3, no. 11 (2008):1935-1938,
https://hdl.handle.net/21.15107/rcub_farfar_1054 .

TY  - JOUR
AU  - Rosić, Nedeljka
AU  - Momcilović, I.
AU  - Kovačević, Nada
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/725
AB  - Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.
PB  - Springer, Dordrecht
T2  - Biologia Plantarum
T1  - Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones
VL  - 50
IS  - 4
SP  - 514
EP  - 518
DO  - 10.1007/s10535-006-0081-6
ER  - 

@article{
author = "Rosić, Nedeljka and Momcilović, I. and Kovačević, Nada and Grubišić, Dragoljub",
year = "2006",
abstract = "Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.",
publisher = "Springer, Dordrecht",
journal = "Biologia Plantarum",
title = "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones",
volume = "50",
number = "4",
pages = "514-518",
doi = "10.1007/s10535-006-0081-6"
}

Rosić, N., Momcilović, I., Kovačević, N.,& Grubišić, D.. (2006). Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum
Springer, Dordrecht., 50(4), 514-518.
https://doi.org/10.1007/s10535-006-0081-6

Rosić N, Momcilović I, Kovačević N, Grubišić D. Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum. 2006;50(4):514-518.
doi:10.1007/s10535-006-0081-6 .

Rosić, Nedeljka, Momcilović, I., Kovačević, Nada, Grubišić, Dragoljub, "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones" in Biologia Plantarum, 50, no. 4 (2006):514-518,
https://doi.org/10.1007/s10535-006-0081-6 . .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Grubišić, Dragoljub
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/620
AB  - This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Biology
T1  - In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production
VL  - 43
IS  - 5
SP  - 420
EP  - 424
DO  - 10.1080/13880200590963691
ER  - 

@article{
author = "Kovačević, Nada and Grubišić, Dragoljub",
year = "2005",
abstract = "This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Biology",
title = "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production",
volume = "43",
number = "5",
pages = "420-424",
doi = "10.1080/13880200590963691"
}

Kovačević, N.,& Grubišić, D.. (2005). In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology
Taylor & Francis Ltd, Abingdon., 43(5), 420-424.
https://doi.org/10.1080/13880200590963691

Kovačević N, Grubišić D. In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology. 2005;43(5):420-424.
doi:10.1080/13880200590963691 .

Kovačević, Nada, Grubišić, Dragoljub, "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production" in Pharmaceutical Biology, 43, no. 5 (2005):420-424,
https://doi.org/10.1080/13880200590963691 . .

TY  - JOUR
AU  - Ristić, M
AU  - Soković, Marina
AU  - Grubišić, Dragoljub
AU  - Kovačević, Nada
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/531
AB  - The essential oil of Achillea atrata L. and its major component, 1,8-cineole, were evaluated for their antifungal activity by the mycelial growth method against 18 micromycetes (Alternaria alternata, Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Cladosporium cladosporioides, Fusarium tricinctum, Penicillium ochrochloron, P. funiculosum, Phomopsis helianthi, Trichoderma viride, Trichophyton mentagrophytes, T. rubrum, T tonsurans, Epidermophyton floccosum, Microsporum canis, M. gypseum). The oil exhibited strong inhibitory action against all tested micromycetes with MIC values of 2.0-8.0 muL/mL.
PB  - Allured Publ Corp, Carol Stream
T2  - Journal of Essential Oil Research
T1  - Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.
VL  - 16
IS  - 1
SP  - 75
EP  - 78
DO  - 10.1080/10412905.2004.9698654
ER  - 

@article{
author = "Ristić, M and Soković, Marina and Grubišić, Dragoljub and Kovačević, Nada",
year = "2004",
abstract = "The essential oil of Achillea atrata L. and its major component, 1,8-cineole, were evaluated for their antifungal activity by the mycelial growth method against 18 micromycetes (Alternaria alternata, Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Cladosporium cladosporioides, Fusarium tricinctum, Penicillium ochrochloron, P. funiculosum, Phomopsis helianthi, Trichoderma viride, Trichophyton mentagrophytes, T. rubrum, T tonsurans, Epidermophyton floccosum, Microsporum canis, M. gypseum). The oil exhibited strong inhibitory action against all tested micromycetes with MIC values of 2.0-8.0 muL/mL.",
publisher = "Allured Publ Corp, Carol Stream",
journal = "Journal of Essential Oil Research",
title = "Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.",
volume = "16",
number = "1",
pages = "75-78",
doi = "10.1080/10412905.2004.9698654"
}

Ristić, M., Soković, M., Grubišić, D.,& Kovačević, N.. (2004). Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.. in Journal of Essential Oil Research
Allured Publ Corp, Carol Stream., 16(1), 75-78.
https://doi.org/10.1080/10412905.2004.9698654

Ristić M, Soković M, Grubišić D, Kovačević N. Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.. in Journal of Essential Oil Research. 2004;16(1):75-78.
doi:10.1080/10412905.2004.9698654 .

Ristić, M, Soković, Marina, Grubišić, Dragoljub, Kovačević, Nada, "Chemical analysis and antifungal activity of the essential oil of Achillea atrata L." in Journal of Essential Oil Research, 16, no. 1 (2004):75-78,
https://doi.org/10.1080/10412905.2004.9698654 . .

TY  - JOUR
AU  - Rosić, Nedeljka
AU  - Momčilović, Ivana D.
AU  - Kovačević, Nada
AU  - Grubišić, Dragoljub
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/276
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro cultures of Rhamnus fallax Boiss., (Rhamnaceae) and anthraquinone production
VL  - 52
IS  - 2
SP  - -1
EP  - -1
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_61
ER  - 

@article{
author = "Rosić, Nedeljka and Momčilović, Ivana D. and Kovačević, Nada and Grubišić, Dragoljub",
year = "2000",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro cultures of Rhamnus fallax Boiss., (Rhamnaceae) and anthraquinone production",
volume = "52",
number = "2",
pages = "-1--1",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_61"
}

Rosić, N., Momčilović, I. D., Kovačević, N.,& Grubišić, D.. (2000). In vitro cultures of Rhamnus fallax Boiss., (Rhamnaceae) and anthraquinone production. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 52(2), -1--1.
https://hdl.handle.net/21.15107/rcub_ibiss_61

Rosić N, Momčilović ID, Kovačević N, Grubišić D. In vitro cultures of Rhamnus fallax Boiss., (Rhamnaceae) and anthraquinone production. in Archives of Biological Sciences. 2000;52(2):-1--1.
https://hdl.handle.net/21.15107/rcub_ibiss_61 .

Rosić, Nedeljka, Momčilović, Ivana D., Kovačević, Nada, Grubišić, Dragoljub, "In vitro cultures of Rhamnus fallax Boiss., (Rhamnaceae) and anthraquinone production" in Archives of Biological Sciences, 52, no. 2 (2000):-1--1,
https://hdl.handle.net/21.15107/rcub_ibiss_61 .

TY  - JOUR
AU  - Tasić, Slavoljub R
AU  - Ristić, Mihailo
AU  - Menković, Nebojša
AU  - Kovačević, Nada
AU  - Grubišić, Dragoljub
AU  - Đoković, Dejan D.
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/209
AB  - Achillea chrysocoma Friv. (Asteraceae) is an endemic plant of the Balkan peninsula. Herb of this yarrow contained up to 0.2% of essential oil. The oil was analyzed by GC and GC/MS and 48 of the 79 detected peaks were identified. The major constituent was 1,8-cineole (17.0%).
T2  - Journal of Essential Oil Research
T1  - Endemic plant species of sara mountain. Part VI. Essential oil of achillea chrysocoma
VL  - 10
IS  - 3
SP  - 329
EP  - 332
DO  - 10.1080/10412905.1998.9700912
ER  - 

@article{
author = "Tasić, Slavoljub R and Ristić, Mihailo and Menković, Nebojša and Kovačević, Nada and Grubišić, Dragoljub and Đoković, Dejan D.",
year = "1998",
abstract = "Achillea chrysocoma Friv. (Asteraceae) is an endemic plant of the Balkan peninsula. Herb of this yarrow contained up to 0.2% of essential oil. The oil was analyzed by GC and GC/MS and 48 of the 79 detected peaks were identified. The major constituent was 1,8-cineole (17.0%).",
journal = "Journal of Essential Oil Research",
title = "Endemic plant species of sara mountain. Part VI. Essential oil of achillea chrysocoma",
volume = "10",
number = "3",
pages = "329-332",
doi = "10.1080/10412905.1998.9700912"
}

Tasić, S. R., Ristić, M., Menković, N., Kovačević, N., Grubišić, D.,& Đoković, D. D.. (1998). Endemic plant species of sara mountain. Part VI. Essential oil of achillea chrysocoma. in Journal of Essential Oil Research, 10(3), 329-332.
https://doi.org/10.1080/10412905.1998.9700912

Tasić SR, Ristić M, Menković N, Kovačević N, Grubišić D, Đoković DD. Endemic plant species of sara mountain. Part VI. Essential oil of achillea chrysocoma. in Journal of Essential Oil Research. 1998;10(3):329-332.
doi:10.1080/10412905.1998.9700912 .

Tasić, Slavoljub R, Ristić, Mihailo, Menković, Nebojša, Kovačević, Nada, Grubišić, Dragoljub, Đoković, Dejan D., "Endemic plant species of sara mountain. Part VI. Essential oil of achillea chrysocoma" in Journal of Essential Oil Research, 10, no. 3 (1998):329-332,
https://doi.org/10.1080/10412905.1998.9700912 . .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Doković, D
AU  - Ristić, Mihailo
AU  - Menković, Nebojša
AU  - Tasić, S
AU  - Grubišić, Dragoljub
PY  - 1997
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/167
AB  - Two flavonoid aglycones were isolated from the resin obtained by washing dry herb of Achillea atrata subspec. multifida with chloroform. The spectral characteristics (UV, MS, 1 H NMR) of these aglycones indicated that they belong to the highly methylated group of 6,8-dihydroxy flavone derivatives, not so frequent in the genus of Achillea. According to their spectral characteristics and literature data, the one was indentified as 5,8-dihydroxy-6,7,4′-trimethoxy flavone (pedunculin), while the structure of the second compound was not finally clarified. Based on its spectral data it could be 5,7,3′-trihydroxy-6,8,4′-trimethoxy flavone or 5,7,8-trihydroxy-6,3′,4′-trimethoxy flavone.
PB  - Medical Information Center
T2  - Pharmacia
T1  - Flavonoids from the herb resin of Achillea atrata L. Subspec, multifida (DC.) HEIM
VL  - 44
IS  - 1
SP  - 14
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_farfar_167
ER  - 

@article{
author = "Kovačević, Nada and Doković, D and Ristić, Mihailo and Menković, Nebojša and Tasić, S and Grubišić, Dragoljub",
year = "1997",
abstract = "Two flavonoid aglycones were isolated from the resin obtained by washing dry herb of Achillea atrata subspec. multifida with chloroform. The spectral characteristics (UV, MS, 1 H NMR) of these aglycones indicated that they belong to the highly methylated group of 6,8-dihydroxy flavone derivatives, not so frequent in the genus of Achillea. According to their spectral characteristics and literature data, the one was indentified as 5,8-dihydroxy-6,7,4′-trimethoxy flavone (pedunculin), while the structure of the second compound was not finally clarified. Based on its spectral data it could be 5,7,3′-trihydroxy-6,8,4′-trimethoxy flavone or 5,7,8-trihydroxy-6,3′,4′-trimethoxy flavone.",
publisher = "Medical Information Center",
journal = "Pharmacia",
title = "Flavonoids from the herb resin of Achillea atrata L. Subspec, multifida (DC.) HEIM",
volume = "44",
number = "1",
pages = "14-16",
url = "https://hdl.handle.net/21.15107/rcub_farfar_167"
}

Kovačević, N., Doković, D., Ristić, M., Menković, N., Tasić, S.,& Grubišić, D.. (1997). Flavonoids from the herb resin of Achillea atrata L. Subspec, multifida (DC.) HEIM. in Pharmacia
Medical Information Center., 44(1), 14-16.
https://hdl.handle.net/21.15107/rcub_farfar_167

Kovačević N, Doković D, Ristić M, Menković N, Tasić S, Grubišić D. Flavonoids from the herb resin of Achillea atrata L. Subspec, multifida (DC.) HEIM. in Pharmacia. 1997;44(1):14-16.
https://hdl.handle.net/21.15107/rcub_farfar_167 .

Kovačević, Nada, Doković, D, Ristić, Mihailo, Menković, Nebojša, Tasić, S, Grubišić, Dragoljub, "Flavonoids from the herb resin of Achillea atrata L. Subspec, multifida (DC.) HEIM" in Pharmacia, 44, no. 1 (1997):14-16,
https://hdl.handle.net/21.15107/rcub_farfar_167 .

TY  - JOUR
AU  - Sajc, L
AU  - Vunjak-Novaković, G
AU  - Grubišić, Dragoljub
AU  - Kovačević, Nada
AU  - Vuković, D
AU  - Bugarski, Branko
PY  - 1995
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/126
AB  - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
T2  - Applied Microbiology and Biotechnology
T1  - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
VL  - 43
IS  - 3
SP  - 416
EP  - 423
DO  - 10.1007/BF00218443
ER  - 

@article{
author = "Sajc, L and Vunjak-Novaković, G and Grubišić, Dragoljub and Kovačević, Nada and Vuković, D and Bugarski, Branko",
year = "1995",
abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.",
journal = "Applied Microbiology and Biotechnology",
title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor",
volume = "43",
number = "3",
pages = "416-423",
doi = "10.1007/BF00218443"
}

Sajc, L., Vunjak-Novaković, G., Grubišić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology, 43(3), 416-423.
https://doi.org/10.1007/BF00218443

Sajc L, Vunjak-Novaković G, Grubišić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423.
doi:10.1007/BF00218443 .

Sajc, L, Vunjak-Novaković, G, Grubišić, Dragoljub, Kovačević, Nada, Vuković, D, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423,
https://doi.org/10.1007/BF00218443 . .