@article{
author = "Vasić, Marija and Topić, Aleksandra and Marković, Bojan and Milinković, Neda and Dinčić, Evica",
year = "2023",
abstract = "Background: Multiple sclerosis (MS) is characterized by
inflammation, demyelination and axonal degeneration.
Oxidative stress (OS) plays a significant role in the pathogenesis of the disease. The aim of the study was to examine the association between OS and smoking on the MS
development.
Methods: The study included 175 patients with relapsing-
remitting multiple sclerosis (RRMS) (76 males, 99 females)
and 254 healthy subjects (81 males and 173 females).
Oxidative stress biomarkers in serum, Total Antioxidant
Status (TAS) and Total Oxidative Status (TOS) were determined spectrophotometrically. Oxidative Stress Index (OSI)
was calculated as the ratio of TOS and TAS. Urinary 8-oxo-
7,8-dihydro-2’-deoxyguanosine were determined by
HPLC-MS/MS and expressed as 8-oxodG/creatinine.
Results: In females with RRMS were higher TOS, OSI and
8-oxodG/creatinine than in females in control group. The
group of males with RRMS had lower level of TAS than the
males in control group. Higher levels of 8-oxodG/creatinine was obtained in active, passive and former smokers
with RRMS than in control group with the same exposition
to tobacco smoke. Independent predictors of MS are passive smoking, increased OSI and increased levels of urinary
8-oxodG/creatinine.
Conclusions: Our results demonstrate that the OS parameters should be included in the assessment of the risk for MS
development. Due to the more sensitivity to oxidative
stress, females may be at higher risk of MS development.
This data indicates the importance of introducing the
antioxidant therapy as a complementary treatment in
patients with RRMS., Uvod: Multipla skleroza (MS) se karakteriše upalom, demijelinizacijom i degeneracijom aksona. Oksidativni stres (OS) igra značajnu ulogu u patogenezi bolesti. Cilj studije je bio da se ispita povezanost OS i pušenja na razvoj MS. Metode: Studija je obuhvatila 175 pacijenata sa relapsnoremitentnom multiplom sklerozom (RRMS) (76 muškaraca, 99 žena) i 254 zdrava ispitanika (81 muškarac i 173 žene). Biomarkeri oksidativnog stresa u serumu, ukupni antioksidativni status (TAS) i ukupni oksidativni status (TOS) su određeni spektrofotometrijski. Indeks oksidativnog stresa (OSI) je izračunat kao odnos TOS i TAS. Urinarni 8-okso7,8-dihidro-2'-deoksiguanozin je određen HPLC-MS/MS i izražen kao 8-oksoG/kreatinin. Rezultati: Kod žena sa RRMS bili su viši TOS, OSI i 8okodG/kreatinin nego kod žena u kontrolnoj grupi. Grupa muškaraca sa RRMS imala je niži nivo TAS od muškaraca u kontrolnoj grupi. Veći nivoi 8-okodG/kreatinina su dobijeni kod aktivnih, pasivnih i bivših pušača sa RRMS nego u kontrolnoj grupi sa istom izloženošću duvanskom dimu. Nezavisni prediktori MS su pasivno pušenje, povećan OSI i povećani nivoi 8-okodG/kreatinina u urinu. Zaključak: Naši rezultati pokazuju da parametre OS treba uključiti u procenu rizika za razvoj MS. Zbog veće osetljivosti na oksidativni stres, žene mogu biti izložene većem riziku od razvoja MS. Ovi podaci ukazuju na značaj uvođenja antioksidativne terapije kao komplementarnog lečenja kod pacijenata sa RRMS.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Oxidative stress-related risk of the multiple sclerosis development, Rizik razvoja multiple skleroze povezan sa oksidativnim stresom",
volume = "42",
number = "1",
pages = "1-8",
doi = "10.5937/jomb0-37546"
}
