TY  - JOUR
AU  - Tomanić, Dragana
AU  - Božin, Biljana
AU  - Kladar, Nebojša
AU  - Stanojević, Jovan
AU  - Čabarkapa, Ivana
AU  - Stilinović, Nebojša
AU  - Apić, Jelena
AU  - Božić, Dragana
AU  - Kovačević, Zorana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4267
AB  - Mastitis is considered to be one of the most important diseases of dairy cows in terms of health, production, and economy. Being the most common cause of antibiotic consumption in dairy cows, treatment of this disease is one of the biggest challenges in the veterinary profession as an increasing number of pathogens develop resistance to antibiotics used in the treatment. Therefore, new alternative approaches for limiting the use of antibiotics in livestock are required. For this reason, our study aimed to investigate prevalence of environmental mastitis associated bacterial strains, as well as the sensitivity of isolated strains to different antibiotics. Additionally, the therapeutic potential of three essential oils (EOs) was tested against bovine Serratia spp. and Proteus spp. mastitis pathogens, based on their chemical composition, as well as antibacterial potential. The study was carried out on 81 milk samples collected from dairy cows with mastitis. In order to determine prevalence of S. marcescens and P. mirabilis, microbiological isolation and identification were performed. Antimicrobial susceptibility testing was performed by disk diffusion method and the microdilution method was used to determine the antibacterial activity of selected EOs. In the oregano EO, a total of 23 compounds were detected, with carvacrol as a dominant component (78.94%). A total of 26 components were present in the EO of common thyme, where thymol was the most abundant compound (46.37%). Thymol also dominated (55.11%) the wild thyme EO. All tested EOs displayed antibacterial activity against all strains to different extents, while wild and common thyme EOs were the most effective. It could be concluded that the tested EOs represent promising therapeutic candidates for effective non-antibiotic treatment of mastitis.
PB  - MDPI
T2  - Antibiotics
T1  - Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils
VL  - 11
IS  - 8
DO  - 10.3390/antibiotics11081077
ER  - 

@article{
author = "Tomanić, Dragana and Božin, Biljana and Kladar, Nebojša and Stanojević, Jovan and Čabarkapa, Ivana and Stilinović, Nebojša and Apić, Jelena and Božić, Dragana and Kovačević, Zorana",
year = "2022",
abstract = "Mastitis is considered to be one of the most important diseases of dairy cows in terms of health, production, and economy. Being the most common cause of antibiotic consumption in dairy cows, treatment of this disease is one of the biggest challenges in the veterinary profession as an increasing number of pathogens develop resistance to antibiotics used in the treatment. Therefore, new alternative approaches for limiting the use of antibiotics in livestock are required. For this reason, our study aimed to investigate prevalence of environmental mastitis associated bacterial strains, as well as the sensitivity of isolated strains to different antibiotics. Additionally, the therapeutic potential of three essential oils (EOs) was tested against bovine Serratia spp. and Proteus spp. mastitis pathogens, based on their chemical composition, as well as antibacterial potential. The study was carried out on 81 milk samples collected from dairy cows with mastitis. In order to determine prevalence of S. marcescens and P. mirabilis, microbiological isolation and identification were performed. Antimicrobial susceptibility testing was performed by disk diffusion method and the microdilution method was used to determine the antibacterial activity of selected EOs. In the oregano EO, a total of 23 compounds were detected, with carvacrol as a dominant component (78.94%). A total of 26 components were present in the EO of common thyme, where thymol was the most abundant compound (46.37%). Thymol also dominated (55.11%) the wild thyme EO. All tested EOs displayed antibacterial activity against all strains to different extents, while wild and common thyme EOs were the most effective. It could be concluded that the tested EOs represent promising therapeutic candidates for effective non-antibiotic treatment of mastitis.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils",
volume = "11",
number = "8",
doi = "10.3390/antibiotics11081077"
}

Tomanić, D., Božin, B., Kladar, N., Stanojević, J., Čabarkapa, I., Stilinović, N., Apić, J., Božić, D.,& Kovačević, Z.. (2022). Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils. in Antibiotics
MDPI., 11(8).
https://doi.org/10.3390/antibiotics11081077

Tomanić D, Božin B, Kladar N, Stanojević J, Čabarkapa I, Stilinović N, Apić J, Božić D, Kovačević Z. Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils. in Antibiotics. 2022;11(8).
doi:10.3390/antibiotics11081077 .

Tomanić, Dragana, Božin, Biljana, Kladar, Nebojša, Stanojević, Jovan, Čabarkapa, Ivana, Stilinović, Nebojša, Apić, Jelena, Božić, Dragana, Kovačević, Zorana, "Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils" in Antibiotics, 11, no. 8 (2022),
https://doi.org/10.3390/antibiotics11081077 . .