TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojsa
AU  - Ninić, Ana
AU  - Kotur-Stevuljević, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3969
AB  - Oxidative stress is assumed to be the underlying feature of non-alcoholic fatty liver disease (NAFLD). To our knowledge, the mutual involvement of redox status homeostasis parameters [i.e., advanced oxidation protein products (AOPP), pro-oxidant-antioxidant balance (PAB), total oxidant status (TOS), total antioxidant status (TAS) and oxidative-stress index (OSI)] and cardiometabolic biomarkers in subjects with NAFLD has not been examined yet. Accordingly, we aimed to investigate this potential relationship. A total of 122 subjects with NAFLD were compared with 56 participants without NAFLD. The diagnosis of NAFLD was confirmed by abdominal ultrasound. Anthropometric and biochemical parameters were measured. OSI, Castelli’s Risk Index I (CRI-I) and Castelli’s Risk Index II (CRI-II) were calculated. Univariate and multivariate binary logistic regression analysis were used to test the predictions of oxidative stress and cardiometabolic markers, respectively for NAFLD. Principal component analysis (PCA) was applied to explore its mutual effect on NAFLD status. Significant positive associations of CRI-I, CRI-II, high sensitivity C-reactive protein (hsCRP) and AOPP with NAFLD were found. PCA analysis extracted 3 significant factors: Oxidative stress-cardiometabolic related factor (i.e., triglycerides, AOPP, HDL-c and HbA1c)-explained 36% of variance; Pro-oxidants related factor (i.e., TOS and PAB)-explained 17% of variance; and Antioxidants related factor (i.e., TAS)- explained 15% of variance of the tested parameters. Moreover, binary logistic regression analysis revealed significant predictive ability of Oxidative stress-cardiometabolic related factor (p < 0.001) and Pro-oxidants related factor (p < 0.05) for NAFLD status. In addition to oxidative stress (i.e., determined by higher AOPP levels), dyslipidemia (i.e., determined by higher lipid indexes: CRI-I and CRI-II) and inflammation (determined by higher hsCRP) are independently related to NAFLD status. The mutual involvement of pro-oxidants (i.e., TOS and PAB), or the joint involvement of pro-oxidants (i.e., AOPP) and cardiometabolic parameters (i.e., HbA1c, triglycerides and HDL-c) can differentiate subjects with NAFLD from those individuals without this metabolic disorder. New studies are needed to validate our results in order to find the best therapeutic approach for NAFLD.
PB  - Nature Research
T2  - Scientific Reports
T1  - Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease
VL  - 11
IS  - 1
DO  - 10.1038/s41598-021-97686-6
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojsa and Ninić, Ana and Kotur-Stevuljević, Jelena",
year = "2021",
abstract = "Oxidative stress is assumed to be the underlying feature of non-alcoholic fatty liver disease (NAFLD). To our knowledge, the mutual involvement of redox status homeostasis parameters [i.e., advanced oxidation protein products (AOPP), pro-oxidant-antioxidant balance (PAB), total oxidant status (TOS), total antioxidant status (TAS) and oxidative-stress index (OSI)] and cardiometabolic biomarkers in subjects with NAFLD has not been examined yet. Accordingly, we aimed to investigate this potential relationship. A total of 122 subjects with NAFLD were compared with 56 participants without NAFLD. The diagnosis of NAFLD was confirmed by abdominal ultrasound. Anthropometric and biochemical parameters were measured. OSI, Castelli’s Risk Index I (CRI-I) and Castelli’s Risk Index II (CRI-II) were calculated. Univariate and multivariate binary logistic regression analysis were used to test the predictions of oxidative stress and cardiometabolic markers, respectively for NAFLD. Principal component analysis (PCA) was applied to explore its mutual effect on NAFLD status. Significant positive associations of CRI-I, CRI-II, high sensitivity C-reactive protein (hsCRP) and AOPP with NAFLD were found. PCA analysis extracted 3 significant factors: Oxidative stress-cardiometabolic related factor (i.e., triglycerides, AOPP, HDL-c and HbA1c)-explained 36% of variance; Pro-oxidants related factor (i.e., TOS and PAB)-explained 17% of variance; and Antioxidants related factor (i.e., TAS)- explained 15% of variance of the tested parameters. Moreover, binary logistic regression analysis revealed significant predictive ability of Oxidative stress-cardiometabolic related factor (p < 0.001) and Pro-oxidants related factor (p < 0.05) for NAFLD status. In addition to oxidative stress (i.e., determined by higher AOPP levels), dyslipidemia (i.e., determined by higher lipid indexes: CRI-I and CRI-II) and inflammation (determined by higher hsCRP) are independently related to NAFLD status. The mutual involvement of pro-oxidants (i.e., TOS and PAB), or the joint involvement of pro-oxidants (i.e., AOPP) and cardiometabolic parameters (i.e., HbA1c, triglycerides and HDL-c) can differentiate subjects with NAFLD from those individuals without this metabolic disorder. New studies are needed to validate our results in order to find the best therapeutic approach for NAFLD.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease",
volume = "11",
number = "1",
doi = "10.1038/s41598-021-97686-6"
}

Klisić, A., Kavarić, N., Ninić, A.,& Kotur-Stevuljević, J.. (2021). Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease. in Scientific Reports
Nature Research., 11(1).
https://doi.org/10.1038/s41598-021-97686-6

Klisić A, Kavarić N, Ninić A, Kotur-Stevuljević J. Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-97686-6 .

Klisić, Aleksandra, Kavarić, Nebojsa, Ninić, Ana, Kotur-Stevuljević, Jelena, "Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-97686-6 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Ninić, Ana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5570
AB  - Objective: Given the fact that pathophysiological background of non-alcoholic fatty liver disease is not completely
elucidated, we aimed to explore the relationship between dyslipidemia, inflammation and oxidative stress with
hepatic steatosis index (HSI) in the cohort of adolescent population in Montenegro.
Patients and Methods: A total of 136 adolescents participated in this cross-sectional study. Anthropometric and
biochemical markers were provided. HSI was calculated. Associations of biochemical parameters with HSI were
tested using univariable and multivariable ordinal regression analysis for HSI tertiles as dependent variable.
Results: Adjusted odds for uric acid (OR=2.641, P=0.007) and for triglycerides, (OR=5.275, P=0.008) showed
their highly significant positive associations with HSI. On the contrary, adjusted odds for bilirubin (OR=0.923,
P=0.015), indicated its significant negative independent association with HSI. Although C-reactive protein
correlated positively with HSI in univariable analysis (OR=1.962, P<0.001), it lost its independent prediction for
HSI in multivariable analysis (OR=1.442, P=0.130).
Conclusion: Serum uric acid, triglycerides and total bilirubin are associated with HSI in adolescents. Longitudinal
studies are needed to confirm the causal relationship between mentioned biomarkers and hepatic steatosis and to
further explore its diagnostic potential in NAFLD in young population.
AB  - Cilj: S obzirom na činjenicu da patofiziološki aspekt nealkoholne steatoze jetre nije u potpunosti razjašnjen, cilj
studije je bio da se ispita povezanost dislipidemije, inflamacije i oksidativnog stresa sa Indeksom masne jetre (IMJ)
u kohorti adolescentne populacije u Crnoj Gori.
Pacijenti i Metode: Ukupno 136 adolescenata je učestvovalo u studiji preseka. Antropometrijski i biohemijski
marker su mereni. IMJ je izračunat. Povezanost biohemijskih parametara sa IMJ ispitana je univarijantnom i
multivarijantnom ordinalnom regresionom analizom, sa tercilnim vrednostima IMJ kao zavisnom varijablom.
Rezultati: Prilagođene odds vrednosti za mokraćnu kiselinu (OR=2,641, P=0,007) i trigliceride (OR=5,275,
P=0,008) pokazali su visoko značajnu pozitivnu povezanost sa IMJ. Suprotno tome, prilagođene odds vrednosti za
bilirubin (OR=0,923, P=0,015) pokazale su značajnu negativnu povezanost sa IMJ. Premda su vrednosti C-
reaktivnog proteina pozitivno korelirale sa IMJ u univarijantnoj (OR=1,962, P<0,001), isti je izgubio nezavisnu
predikciju za IMJ u multivarijantnoj analizi (OR=1,442, P=0,130).
Zaključak: Serumske vrednosti mokraćne kiseline, triglicerida i ukupnog bilirubina su povezane sa IMJ u
adolescenata. Longitudinalne studije su potrebne da potvrde uzročnu povezanost između pomenutih biomarkera i
steatoze jetre, kao i da dalje ispitaju njihov dijagnostički potencijal za ovo oboljenje u mlađoj populaciji.
PB  - Udruženje preventivne pedijatrije Srbije
T2  - Preventivna pedijatrija
T1  - Serum uric acid, triglycerides and total bilirubin are associated with Hepatic Steatosis Index in adolescent population
T1  - Serumske vrednosti mokraćne kiseline, triglicerida i ukupnog bilirubina su povezane sa Indeksom masne jetre u adolescentnoj populaciji
VL  - 6
IS  - 1-2
SP  - 71
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5570
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Ninić, Ana",
year = "2020",
abstract = "Objective: Given the fact that pathophysiological background of non-alcoholic fatty liver disease is not completely
elucidated, we aimed to explore the relationship between dyslipidemia, inflammation and oxidative stress with
hepatic steatosis index (HSI) in the cohort of adolescent population in Montenegro.
Patients and Methods: A total of 136 adolescents participated in this cross-sectional study. Anthropometric and
biochemical markers were provided. HSI was calculated. Associations of biochemical parameters with HSI were
tested using univariable and multivariable ordinal regression analysis for HSI tertiles as dependent variable.
Results: Adjusted odds for uric acid (OR=2.641, P=0.007) and for triglycerides, (OR=5.275, P=0.008) showed
their highly significant positive associations with HSI. On the contrary, adjusted odds for bilirubin (OR=0.923,
P=0.015), indicated its significant negative independent association with HSI. Although C-reactive protein
correlated positively with HSI in univariable analysis (OR=1.962, P<0.001), it lost its independent prediction for
HSI in multivariable analysis (OR=1.442, P=0.130).
Conclusion: Serum uric acid, triglycerides and total bilirubin are associated with HSI in adolescents. Longitudinal
studies are needed to confirm the causal relationship between mentioned biomarkers and hepatic steatosis and to
further explore its diagnostic potential in NAFLD in young population., Cilj: S obzirom na činjenicu da patofiziološki aspekt nealkoholne steatoze jetre nije u potpunosti razjašnjen, cilj
studije je bio da se ispita povezanost dislipidemije, inflamacije i oksidativnog stresa sa Indeksom masne jetre (IMJ)
u kohorti adolescentne populacije u Crnoj Gori.
Pacijenti i Metode: Ukupno 136 adolescenata je učestvovalo u studiji preseka. Antropometrijski i biohemijski
marker su mereni. IMJ je izračunat. Povezanost biohemijskih parametara sa IMJ ispitana je univarijantnom i
multivarijantnom ordinalnom regresionom analizom, sa tercilnim vrednostima IMJ kao zavisnom varijablom.
Rezultati: Prilagođene odds vrednosti za mokraćnu kiselinu (OR=2,641, P=0,007) i trigliceride (OR=5,275,
P=0,008) pokazali su visoko značajnu pozitivnu povezanost sa IMJ. Suprotno tome, prilagođene odds vrednosti za
bilirubin (OR=0,923, P=0,015) pokazale su značajnu negativnu povezanost sa IMJ. Premda su vrednosti C-
reaktivnog proteina pozitivno korelirale sa IMJ u univarijantnoj (OR=1,962, P<0,001), isti je izgubio nezavisnu
predikciju za IMJ u multivarijantnoj analizi (OR=1,442, P=0,130).
Zaključak: Serumske vrednosti mokraćne kiseline, triglicerida i ukupnog bilirubina su povezane sa IMJ u
adolescenata. Longitudinalne studije su potrebne da potvrde uzročnu povezanost između pomenutih biomarkera i
steatoze jetre, kao i da dalje ispitaju njihov dijagnostički potencijal za ovo oboljenje u mlađoj populaciji.",
publisher = "Udruženje preventivne pedijatrije Srbije",
journal = "Preventivna pedijatrija",
title = "Serum uric acid, triglycerides and total bilirubin are associated with Hepatic Steatosis Index in adolescent population, Serumske vrednosti mokraćne kiseline, triglicerida i ukupnog bilirubina su povezane sa Indeksom masne jetre u adolescentnoj populaciji",
volume = "6",
number = "1-2",
pages = "71-76",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5570"
}

Klisić, A., Kavarić, N.,& Ninić, A.. (2020). Serum uric acid, triglycerides and total bilirubin are associated with Hepatic Steatosis Index in adolescent population. in Preventivna pedijatrija
Udruženje preventivne pedijatrije Srbije., 6(1-2), 71-76.
https://hdl.handle.net/21.15107/rcub_farfar_5570

Klisić A, Kavarić N, Ninić A. Serum uric acid, triglycerides and total bilirubin are associated with Hepatic Steatosis Index in adolescent population. in Preventivna pedijatrija. 2020;6(1-2):71-76.
https://hdl.handle.net/21.15107/rcub_farfar_5570 .

Klisić, Aleksandra, Kavarić, Nebojša, Ninić, Ana, "Serum uric acid, triglycerides and total bilirubin are associated with Hepatic Steatosis Index in adolescent population" in Preventivna pedijatrija, 6, no. 1-2 (2020):71-76,
https://hdl.handle.net/21.15107/rcub_farfar_5570 .