TY  - CONF
AU  - Ćuruvija, Ivana
AU  - Bufan, Biljana
AU  - Đorović, Emilija
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Marković, Milica
AU  - Đuretić, Jasmina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5132
AB  - Aims Ageing affects N-methyl-D-aspartate receptors (NMDARs), their expression and function in neuronal and non-neuronal 
cells. Contribution of NMDARs to pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been investigated 
but further study is still needed. The aim of this study was to determine whether ageing affects the role of NMDARs in EAE. 
Methods Memantine, a non-competitive NMDAR antagonist which limits pathological activity of NMDARs while sparing 
normal synaptic activity, was administered orally from day 7 after immunization to 3- and 24-month-old female Dark Agouti 
rats. The animals were sacrificed at the peak of the disease. Spinal cord mononuclear cells were analyzed by flow cytometry. 
Brain tissue was collected for biochemical analysis of redox status and RT-qPCR. Results Semiprophylactic administration of 
memantine ameliorated clinical disease course, with greater effect in aged rats. Memantine reduced the number, frequency, 
and reactivation of CD4+ T lymphocytes and increased the relative percentage of CX3CR1-expressing microglia in spinal 
cord, but to a greater extent in aged rats. Additionally, analysis of brain redox status parameters showed that memantine was 
more effective in reducing superoxide anion radical, malondialdehyde and advanced oxidation protein products in aged rats 
than in young ones. In accordance with previous findings, NMDAR inhibition by memantine decreased NADPH oxidase and 
IL-1β expression and increased the nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 expression, to a greater 
extent in aged rats. Conclusions The involvement of NMDARs in the pathogenesis of EAE was age-dependent, being more 
pronounced in aged than in young rats.
PB  - Federation of European Neuroscience Societies (FENS)
C3  - FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts
T1  - Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis
SP  - S05-250
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5132
ER  - 

@conference{
author = "Ćuruvija, Ivana and Bufan, Biljana and Đorović, Emilija and Blagojević, Veljko and Grujić-Milanović, Jelica and Marković, Milica and Đuretić, Jasmina",
year = "2022",
abstract = "Aims Ageing affects N-methyl-D-aspartate receptors (NMDARs), their expression and function in neuronal and non-neuronal 
cells. Contribution of NMDARs to pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been investigated 
but further study is still needed. The aim of this study was to determine whether ageing affects the role of NMDARs in EAE. 
Methods Memantine, a non-competitive NMDAR antagonist which limits pathological activity of NMDARs while sparing 
normal synaptic activity, was administered orally from day 7 after immunization to 3- and 24-month-old female Dark Agouti 
rats. The animals were sacrificed at the peak of the disease. Spinal cord mononuclear cells were analyzed by flow cytometry. 
Brain tissue was collected for biochemical analysis of redox status and RT-qPCR. Results Semiprophylactic administration of 
memantine ameliorated clinical disease course, with greater effect in aged rats. Memantine reduced the number, frequency, 
and reactivation of CD4+ T lymphocytes and increased the relative percentage of CX3CR1-expressing microglia in spinal 
cord, but to a greater extent in aged rats. Additionally, analysis of brain redox status parameters showed that memantine was 
more effective in reducing superoxide anion radical, malondialdehyde and advanced oxidation protein products in aged rats 
than in young ones. In accordance with previous findings, NMDAR inhibition by memantine decreased NADPH oxidase and 
IL-1β expression and increased the nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 expression, to a greater 
extent in aged rats. Conclusions The involvement of NMDARs in the pathogenesis of EAE was age-dependent, being more 
pronounced in aged than in young rats.",
publisher = "Federation of European Neuroscience Societies (FENS)",
journal = "FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts",
title = "Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis",
pages = "S05-250",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5132"
}

Ćuruvija, I., Bufan, B., Đorović, E., Blagojević, V., Grujić-Milanović, J., Marković, M.,& Đuretić, J.. (2022). Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis. in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts
Federation of European Neuroscience Societies (FENS)., S05-250.
https://hdl.handle.net/21.15107/rcub_farfar_5132

Ćuruvija I, Bufan B, Đorović E, Blagojević V, Grujić-Milanović J, Marković M, Đuretić J. Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis. in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts. 2022;:S05-250.
https://hdl.handle.net/21.15107/rcub_farfar_5132 .

Ćuruvija, Ivana, Bufan, Biljana, Đorović, Emilija, Blagojević, Veljko, Grujić-Milanović, Jelica, Marković, Milica, Đuretić, Jasmina, "Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis" in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts (2022):S05-250,
https://hdl.handle.net/21.15107/rcub_farfar_5132 .

TY  - JOUR
AU  - Nikodijević, Slavomir
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Kosanović, Dejana
AU  - Đukić, Tamara
AU  - Đorđević, Brižita
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4294
AB  - Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
PB  - John Wiley and Sons Inc
T2  - Scandinavian Journal of Immunology
T1  - Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans
VL  - 96
IS  - 6
DO  - 10.1111/sji.13223
ER  - 

@article{
author = "Nikodijević, Slavomir and Blagojević, Veljko and Ćuruvija, Ivana and Kosanović, Dejana and Đukić, Tamara and Đorđević, Brižita and Ilić, Vesna and Minić, Rajna",
year = "2022",
abstract = "Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.",
publisher = "John Wiley and Sons Inc",
journal = "Scandinavian Journal of Immunology",
title = "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans",
volume = "96",
number = "6",
doi = "10.1111/sji.13223"
}

Nikodijević, S., Blagojević, V., Ćuruvija, I., Kosanović, D., Đukić, T., Đorđević, B., Ilić, V.,& Minić, R.. (2022). Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology
John Wiley and Sons Inc., 96(6).
https://doi.org/10.1111/sji.13223

Nikodijević S, Blagojević V, Ćuruvija I, Kosanović D, Đukić T, Đorđević B, Ilić V, Minić R. Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology. 2022;96(6).
doi:10.1111/sji.13223 .

Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Đorđević, Brižita, Ilić, Vesna, Minić, Rajna, "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans" in Scandinavian Journal of Immunology, 96, no. 6 (2022),
https://doi.org/10.1111/sji.13223 . .

TY  - THES
AU  - Ćuruvija, Ivana
PY  - 2022
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9053
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:29011/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/62302985
UR  - https://nardus.mpn.gov.rs/handle/123456789/21381
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4838
AB  - Starenje se povezuje sa razvojem sistemskog, sterilnog hroničnog zapaljenja (engl.„inflammaging”). Malo je podataka o uticaju genetskih faktora i pola na sposobnost makrofaga(Mφ), kao ključnih ćelija urođenog imunskog odgovora, da tokom starenja “kontrolišu”rezoluciju akutnog zapaljenja i time razvoj hroničnog zapaljenja. Ciljevi ove disertacije su bilida se ispita 1) uticaj rane faze reproduktivnog starenja na fenotipske osobine (ekspresijamarkera povezanih sa aktivacijom i poreklom/funkcijom) i funkcijska svojstva (fagocitoza,sinteza inflamatornih medijatora) Mφ “mirne” i inflamirane (delovanjem tioglikolata)peritonealne duplje ženki Albino Oxford (AO) pacova, 2) značaj genetskih faktora zareproduktivnim starenjem uslovljene promene peritonealnih Mφ od značaja za uspešnurezoluciju akutnog zapaljenja i 3) uloga polnih steroida u nastanku ovih promena uporednomanalizom promena kod mužjaka i ženki AO pacova, njihovom analizom kod ženki AO pacovakojima su na kraju reproduktivnog perioda uklonjeni jajnici i ispitivanjem delovanjaestradiola na Mφ mladih i sredovečnih ženki AO pacova in vitro. Rezultati su pokazali da: 1) sesposobnost Mφ ženki AO pacova da “kontrolišu” rezoluciju akutnog zapaljenja menja većtokom rane faze reproduktivnog starenja, kao i da su ove promene sojno specifične (Mφsredovečnih ženki AO pacova koje “uspešnije” stare od ženki Dark Agouti pacova pokazujusvojstva koja se mogu povezati sa boljom “kontrolom” inflamacije); 2) su ove promene polnospecifične (Mφ sredovečnih ženki imaju svojstva koja ukazuju na veći kapacitet da“kontrolišu” inflamaciju od Mφ mužjaka istog uzrasta) i 3) u nastanku promena relevantnih zasposobnost Mφ ženki AO pacova da “kontrolišu” akutnu inflamaciju važnu ulogu imajupromene u delovanju i estradiola i progesterona. Dodatno, ispitavanja in vitro su ukazala da suza uzrasno zavisne promene u sposobnosti Mφ da “kontrolišu” inflamaciju pored promene ukoncentraciji estradiola važne i one u samim Mφ, koje menjaju njihov odgovor na delovanjeestradiola.
AB  - Aging is associated with the development of systemic, sterile chronic inflammation("inflammaging"). Little is known about the influence of genetic factors and sex on the abilityof macrophages (Mφ), as key innate immune cells, to "control" the resolution of acuteinflammation and thus the development of chronic inflammation during aging. The objectivesof this dissertation were to examine 1) the influence of the early phase of reproductive agingon phenotypic characteristics (expression of markers associated with activation andorigin/function) and functional characteristics (phagocytosis, synthesis of inflammatorymediators) of Mφ isolated from “naive” and inflamed (thioglycollate-induced) peritonealcavity of females Albino Oxford (AO) rats; 2) the significance of genetic factors forreproductive aging-related changes of peritoneal Mφ, especially those important forsuccessful resolution of acute inflammation and 3) the role of sex steroids in the occurrence ofthese changes by comparative analysis in males and females of AO rats, their analysis infemale AO rats whose ovaries were removed at the end of the reproductive period and byexamining in vitro effect of estradiol on Mφ from young and middle-aged female AO rats. Theresults showed that: 1) the ability of Mφ from AO females to “control” the resolution of acuteinflammation changes during the early phase of reproductive aging, and these changes arestrain-specific (Mφ from middle-aged AO females that “age more successful” than Dark Agoutifemales, show properties which may be associated with better "control" of inflammation); 2)these changes are sex-specific (Mφ from middle-aged females have a greater capacity to“control” inflammation than Mφ from males of the same age group) and 3) both estradiol andprogesterone play important roles in the ability of Mφ from AO females to “control” acuteinflammation. In vitro studies revealed that, in addition to changes in estradiol concentration,intrinsic changes in Mφ that regulate their response to estradiol action are important for age-dependent changes in Mφ ability to „control“ inflammation.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Polne i sojne specifičnosti promena citokinskog profila makrofaga ženki pacova u reproduktivnom starenju
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21381
ER  - 

@phdthesis{
author = "Ćuruvija, Ivana",
year = "2022",
abstract = "Starenje se povezuje sa razvojem sistemskog, sterilnog hroničnog zapaljenja (engl.„inflammaging”). Malo je podataka o uticaju genetskih faktora i pola na sposobnost makrofaga(Mφ), kao ključnih ćelija urođenog imunskog odgovora, da tokom starenja “kontrolišu”rezoluciju akutnog zapaljenja i time razvoj hroničnog zapaljenja. Ciljevi ove disertacije su bilida se ispita 1) uticaj rane faze reproduktivnog starenja na fenotipske osobine (ekspresijamarkera povezanih sa aktivacijom i poreklom/funkcijom) i funkcijska svojstva (fagocitoza,sinteza inflamatornih medijatora) Mφ “mirne” i inflamirane (delovanjem tioglikolata)peritonealne duplje ženki Albino Oxford (AO) pacova, 2) značaj genetskih faktora zareproduktivnim starenjem uslovljene promene peritonealnih Mφ od značaja za uspešnurezoluciju akutnog zapaljenja i 3) uloga polnih steroida u nastanku ovih promena uporednomanalizom promena kod mužjaka i ženki AO pacova, njihovom analizom kod ženki AO pacovakojima su na kraju reproduktivnog perioda uklonjeni jajnici i ispitivanjem delovanjaestradiola na Mφ mladih i sredovečnih ženki AO pacova in vitro. Rezultati su pokazali da: 1) sesposobnost Mφ ženki AO pacova da “kontrolišu” rezoluciju akutnog zapaljenja menja većtokom rane faze reproduktivnog starenja, kao i da su ove promene sojno specifične (Mφsredovečnih ženki AO pacova koje “uspešnije” stare od ženki Dark Agouti pacova pokazujusvojstva koja se mogu povezati sa boljom “kontrolom” inflamacije); 2) su ove promene polnospecifične (Mφ sredovečnih ženki imaju svojstva koja ukazuju na veći kapacitet da“kontrolišu” inflamaciju od Mφ mužjaka istog uzrasta) i 3) u nastanku promena relevantnih zasposobnost Mφ ženki AO pacova da “kontrolišu” akutnu inflamaciju važnu ulogu imajupromene u delovanju i estradiola i progesterona. Dodatno, ispitavanja in vitro su ukazala da suza uzrasno zavisne promene u sposobnosti Mφ da “kontrolišu” inflamaciju pored promene ukoncentraciji estradiola važne i one u samim Mφ, koje menjaju njihov odgovor na delovanjeestradiola., Aging is associated with the development of systemic, sterile chronic inflammation("inflammaging"). Little is known about the influence of genetic factors and sex on the abilityof macrophages (Mφ), as key innate immune cells, to "control" the resolution of acuteinflammation and thus the development of chronic inflammation during aging. The objectivesof this dissertation were to examine 1) the influence of the early phase of reproductive agingon phenotypic characteristics (expression of markers associated with activation andorigin/function) and functional characteristics (phagocytosis, synthesis of inflammatorymediators) of Mφ isolated from “naive” and inflamed (thioglycollate-induced) peritonealcavity of females Albino Oxford (AO) rats; 2) the significance of genetic factors forreproductive aging-related changes of peritoneal Mφ, especially those important forsuccessful resolution of acute inflammation and 3) the role of sex steroids in the occurrence ofthese changes by comparative analysis in males and females of AO rats, their analysis infemale AO rats whose ovaries were removed at the end of the reproductive period and byexamining in vitro effect of estradiol on Mφ from young and middle-aged female AO rats. Theresults showed that: 1) the ability of Mφ from AO females to “control” the resolution of acuteinflammation changes during the early phase of reproductive aging, and these changes arestrain-specific (Mφ from middle-aged AO females that “age more successful” than Dark Agoutifemales, show properties which may be associated with better "control" of inflammation); 2)these changes are sex-specific (Mφ from middle-aged females have a greater capacity to“control” inflammation than Mφ from males of the same age group) and 3) both estradiol andprogesterone play important roles in the ability of Mφ from AO females to “control” acuteinflammation. In vitro studies revealed that, in addition to changes in estradiol concentration,intrinsic changes in Mφ that regulate their response to estradiol action are important for age-dependent changes in Mφ ability to „control“ inflammation.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Polne i sojne specifičnosti promena citokinskog profila makrofaga ženki pacova u reproduktivnom starenju",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21381"
}

Ćuruvija, I.. (2022). Polne i sojne specifičnosti promena citokinskog profila makrofaga ženki pacova u reproduktivnom starenju. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21381

Ćuruvija I. Polne i sojne specifičnosti promena citokinskog profila makrofaga ženki pacova u reproduktivnom starenju. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_21381 .

Ćuruvija, Ivana, "Polne i sojne specifičnosti promena citokinskog profila makrofaga ženki pacova u reproduktivnom starenju" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_nardus_21381 .