TY  - JOUR
AU  - Dudašova Petrovičova, Olina
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
AU  - Dopsaj, Violeta
AU  - Milinković, Neda
AU  - Dopsaj, Milivoj
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5409
AB  - A randomized, double-blind, placebo-controlled study was conducted to investigate the influence of supplementation with a superoxide dismutase (SOD)-rich plant extract on markers of oxidative stress, zonulin levels and the performance of elite athletes. Participants were 30 international-level rowers, divided into an experimental group (n = 15) and a control group (n = 15). The rowers performed a maximal effort incremental test on a rowing ergometer at the beginning and at the end of the study. Markers of oxidative stress (total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), advanced oxidation protein products (AOPPs), malondialdehyde (MDA), sulfhydryl (SH) groups, bilirubin, uric acid, albumin and zonulin) were determined in serum. A lower TOS (p = 0.010) and OSI (p = 0.004), a lower MDA (p = 0.001) and a higher level of SH groups (p = 0.031) were observed in the experimental group after supplementation. Physical performance was evaluated through metabolic efficiency, taking lactate levels and power output on the ergometer into account. After 6 weeks of supplementation, the relative increase in metabolic efficiency at a 4 mmol/L lactate concentration and maximal effort was significantly higher in the experimental group (p = 0.004 and p = 0.015, respectively). These results suggest that supplementation with a SOD-rich extract promotes lower oxidative stress, better antioxidant protection and, consequently, the better work performance of athletes.
PB  - MDPI
T2  - Metabolites
T1  - How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress
VL  - 13
IS  - 12
DO  - 10.3390/metabo13121200
ER  - 

@article{
author = "Dudašova Petrovičova, Olina and Stanković, Ivan and Đorđević, Brižita and Dopsaj, Violeta and Milinković, Neda and Dopsaj, Milivoj",
year = "2023",
abstract = "A randomized, double-blind, placebo-controlled study was conducted to investigate the influence of supplementation with a superoxide dismutase (SOD)-rich plant extract on markers of oxidative stress, zonulin levels and the performance of elite athletes. Participants were 30 international-level rowers, divided into an experimental group (n = 15) and a control group (n = 15). The rowers performed a maximal effort incremental test on a rowing ergometer at the beginning and at the end of the study. Markers of oxidative stress (total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), advanced oxidation protein products (AOPPs), malondialdehyde (MDA), sulfhydryl (SH) groups, bilirubin, uric acid, albumin and zonulin) were determined in serum. A lower TOS (p = 0.010) and OSI (p = 0.004), a lower MDA (p = 0.001) and a higher level of SH groups (p = 0.031) were observed in the experimental group after supplementation. Physical performance was evaluated through metabolic efficiency, taking lactate levels and power output on the ergometer into account. After 6 weeks of supplementation, the relative increase in metabolic efficiency at a 4 mmol/L lactate concentration and maximal effort was significantly higher in the experimental group (p = 0.004 and p = 0.015, respectively). These results suggest that supplementation with a SOD-rich extract promotes lower oxidative stress, better antioxidant protection and, consequently, the better work performance of athletes.",
publisher = "MDPI",
journal = "Metabolites",
title = "How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress",
volume = "13",
number = "12",
doi = "10.3390/metabo13121200"
}

Dudašova Petrovičova, O., Stanković, I., Đorđević, B., Dopsaj, V., Milinković, N.,& Dopsaj, M.. (2023). How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress. in Metabolites
MDPI., 13(12).
https://doi.org/10.3390/metabo13121200

Dudašova Petrovičova O, Stanković I, Đorđević B, Dopsaj V, Milinković N, Dopsaj M. How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress. in Metabolites. 2023;13(12).
doi:10.3390/metabo13121200 .

Dudašova Petrovičova, Olina, Stanković, Ivan, Đorđević, Brižita, Dopsaj, Violeta, Milinković, Neda, Dopsaj, Milivoj, "How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress" in Metabolites, 13, no. 12 (2023),
https://doi.org/10.3390/metabo13121200 . .

TY  - JOUR
AU  - Trtica, Marko
AU  - Milenković, Branislava
AU  - Janković, Jelena
AU  - Dimić Janjić, Sanja
AU  - Dopuđa Pantić, Vesna
AU  - Dopsaj, Violeta
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4426
AB  - Introduction: Erythrocyte indices LHD and Maf are complementary parameters to complete blood count and have been shown as reliable iron deficiency markers in different clinical settings. The aim of the study was to assess diagnostic performances of LHD and Maf in detecting iron deficiency in nonanaemic stable COPD patients. Methods: A total of 93 nonanaemic stable COPD patients were classified as either iron deficient (ID, N = 15) or non-iron deficient (non-ID, N = 78). Iron deficiency was defined as a ferritin level < 100 μg/L with a transferrin saturation (TSAT) <20%. A complete blood count, including LHD and Maf as well as other relevant inflammation and iron status parameters were obtained for all participants. Results: Both LHD and Maf have shown significant differences between the ID and non-ID group with p =.003 and p =.007 respectively. The AUC for LHD was.744 (95% CI:.626–.863, p =.003) with the best cut-off of 5.85 and sensitivity of 80% (95% CI: 76.0–84.0) and specificity of 61.5% (95% CI: 58.4–64.6). The AUC for Maf was.707 with optimal cut-off value 12.65 and sensitivity of 83.3% (95% CI: 79.1–87.5) and specificity of 60.0% (95% CI: 57.0–63.0). Furthermore, LHD performance was not affected by vitamin B12 status. Conclusion: LHD and Maf are useful for iron deficiency diagnosis in stable COPD patients. LHD was shown to be resistant to vitamin B12 deficiency, which is of substantial importance in specific patient subpopulations. Both parameters are not technology-dependant and do not require additional sample and/or reagent volume, which makes them cost-effective and convenient for everyday use.
PB  - John Wiley and Sons Inc
T2  - International Journal of Laboratory Hematology
T1  - Iron deficiency in nonanaemic COPD patients—Could Low haemoglobin density and Microcytic anaemia factor be usefull?
VL  - 45
IS  - 3
SP  - 394
EP  - 402
DO  - 10.1111/ijlh.14029
ER  - 

@article{
author = "Trtica, Marko and Milenković, Branislava and Janković, Jelena and Dimić Janjić, Sanja and Dopuđa Pantić, Vesna and Dopsaj, Violeta",
year = "2023",
abstract = "Introduction: Erythrocyte indices LHD and Maf are complementary parameters to complete blood count and have been shown as reliable iron deficiency markers in different clinical settings. The aim of the study was to assess diagnostic performances of LHD and Maf in detecting iron deficiency in nonanaemic stable COPD patients. Methods: A total of 93 nonanaemic stable COPD patients were classified as either iron deficient (ID, N = 15) or non-iron deficient (non-ID, N = 78). Iron deficiency was defined as a ferritin level < 100 μg/L with a transferrin saturation (TSAT) <20%. A complete blood count, including LHD and Maf as well as other relevant inflammation and iron status parameters were obtained for all participants. Results: Both LHD and Maf have shown significant differences between the ID and non-ID group with p =.003 and p =.007 respectively. The AUC for LHD was.744 (95% CI:.626–.863, p =.003) with the best cut-off of 5.85 and sensitivity of 80% (95% CI: 76.0–84.0) and specificity of 61.5% (95% CI: 58.4–64.6). The AUC for Maf was.707 with optimal cut-off value 12.65 and sensitivity of 83.3% (95% CI: 79.1–87.5) and specificity of 60.0% (95% CI: 57.0–63.0). Furthermore, LHD performance was not affected by vitamin B12 status. Conclusion: LHD and Maf are useful for iron deficiency diagnosis in stable COPD patients. LHD was shown to be resistant to vitamin B12 deficiency, which is of substantial importance in specific patient subpopulations. Both parameters are not technology-dependant and do not require additional sample and/or reagent volume, which makes them cost-effective and convenient for everyday use.",
publisher = "John Wiley and Sons Inc",
journal = "International Journal of Laboratory Hematology",
title = "Iron deficiency in nonanaemic COPD patients—Could Low haemoglobin density and Microcytic anaemia factor be usefull?",
volume = "45",
number = "3",
pages = "394-402",
doi = "10.1111/ijlh.14029"
}

Trtica, M., Milenković, B., Janković, J., Dimić Janjić, S., Dopuđa Pantić, V.,& Dopsaj, V.. (2023). Iron deficiency in nonanaemic COPD patients—Could Low haemoglobin density and Microcytic anaemia factor be usefull?. in International Journal of Laboratory Hematology
John Wiley and Sons Inc., 45(3), 394-402.
https://doi.org/10.1111/ijlh.14029

Trtica M, Milenković B, Janković J, Dimić Janjić S, Dopuđa Pantić V, Dopsaj V. Iron deficiency in nonanaemic COPD patients—Could Low haemoglobin density and Microcytic anaemia factor be usefull?. in International Journal of Laboratory Hematology. 2023;45(3):394-402.
doi:10.1111/ijlh.14029 .

Trtica, Marko, Milenković, Branislava, Janković, Jelena, Dimić Janjić, Sanja, Dopuđa Pantić, Vesna, Dopsaj, Violeta, "Iron deficiency in nonanaemic COPD patients—Could Low haemoglobin density and Microcytic anaemia factor be usefull?" in International Journal of Laboratory Hematology, 45, no. 3 (2023):394-402,
https://doi.org/10.1111/ijlh.14029 . .

TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanovich, Ljudmila
AU  - Đoković, Aleksandra
AU  - Todić, Brankica
AU  - Dopsaj, Violeta
AU  - Saponjski, Jovica
AU  - Saponjski, Dušan
AU  - Marković, Olivera
AU  - Belizna, Cristina
AU  - Zdravković, Marija
AU  - Marisavljević, Dragomir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4310
AB  - Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients
VL  - 23
IS  - 20
DO  - 10.3390/ijms232012309
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanovich, Ljudmila and Đoković, Aleksandra and Todić, Brankica and Dopsaj, Violeta and Saponjski, Jovica and Saponjski, Dušan and Marković, Olivera and Belizna, Cristina and Zdravković, Marija and Marisavljević, Dragomir",
year = "2022",
abstract = "Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients",
volume = "23",
number = "20",
doi = "10.3390/ijms232012309"
}

Stanisavljević, N., Stojanovich, L., Đoković, A., Todić, B., Dopsaj, V., Saponjski, J., Saponjski, D., Marković, O., Belizna, C., Zdravković, M.,& Marisavljević, D.. (2022). Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences
MDPI., 23(20).
https://doi.org/10.3390/ijms232012309

Stanisavljević N, Stojanovich L, Đoković A, Todić B, Dopsaj V, Saponjski J, Saponjski D, Marković O, Belizna C, Zdravković M, Marisavljević D. Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences. 2022;23(20).
doi:10.3390/ijms232012309 .

Stanisavljević, Nataša, Stojanovich, Ljudmila, Đoković, Aleksandra, Todić, Brankica, Dopsaj, Violeta, Saponjski, Jovica, Saponjski, Dušan, Marković, Olivera, Belizna, Cristina, Zdravković, Marija, Marisavljević, Dragomir, "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients" in International Journal of Molecular Sciences, 23, no. 20 (2022),
https://doi.org/10.3390/ijms232012309 . .

TY  - JOUR
AU  - Dudašova Petrovičova, Olina
AU  - Stanković, Ivan
AU  - Milinković, Neda
AU  - Dopsaj, Violeta
AU  - Đorđević, Brižita
AU  - Dopsaj, Milivoj
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4302
AB  - This study aimed to investigate the effect of supplementation with plant origin superoxide dismutase (SOD), GliSODin, on parameters of muscle damage, metabolic, and work performance at international level rowers. Twenty-eight rowers were included in a randomized, double-blind study. The study was conducted during a 6-week preparation period. At the beginning of the study and after 6 weeks of the supplementation period, all rowers were tested on a rowing ergometer. Blood samples were taken from the antecubital vein before and after every ergometer testing. Muscle damage markers creatine kinase (CK) and lactate dehydrogenase (LDH), total antioxidant capacity (TAC), inflammation parameters interleukin-6 (IL-6), and C-reactive protein (CRP) were measured. Rowing performance was assessed by lactate level in capillary blood and power output on the rowing ergometer. After supplementation, experimental group had significantly lower CK (p = 0.049) and IL-6 (p = 0.035) before and IL-6 (p = 0.050) after exhausting exercise on ergometer. Relative change of power output at 4 mmol/L concentration of lactate in blood, considering the initial and final test, was significantly higher (p = 0.020) in the supplemented group. It was concluded that GliSODin could be considered a good supplement in preventing some deleterious effects of intensive physical activity, including inflammation and muscle damage, and consequently, to enable a better rowing performance of elite rowers.
PB  - MDPI
T2  - Biology
T1  - Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort
VL  - 11
IS  - 10
DO  - 10.3390/biology11101437
ER  - 

@article{
author = "Dudašova Petrovičova, Olina and Stanković, Ivan and Milinković, Neda and Dopsaj, Violeta and Đorđević, Brižita and Dopsaj, Milivoj",
year = "2022",
abstract = "This study aimed to investigate the effect of supplementation with plant origin superoxide dismutase (SOD), GliSODin, on parameters of muscle damage, metabolic, and work performance at international level rowers. Twenty-eight rowers were included in a randomized, double-blind study. The study was conducted during a 6-week preparation period. At the beginning of the study and after 6 weeks of the supplementation period, all rowers were tested on a rowing ergometer. Blood samples were taken from the antecubital vein before and after every ergometer testing. Muscle damage markers creatine kinase (CK) and lactate dehydrogenase (LDH), total antioxidant capacity (TAC), inflammation parameters interleukin-6 (IL-6), and C-reactive protein (CRP) were measured. Rowing performance was assessed by lactate level in capillary blood and power output on the rowing ergometer. After supplementation, experimental group had significantly lower CK (p = 0.049) and IL-6 (p = 0.035) before and IL-6 (p = 0.050) after exhausting exercise on ergometer. Relative change of power output at 4 mmol/L concentration of lactate in blood, considering the initial and final test, was significantly higher (p = 0.020) in the supplemented group. It was concluded that GliSODin could be considered a good supplement in preventing some deleterious effects of intensive physical activity, including inflammation and muscle damage, and consequently, to enable a better rowing performance of elite rowers.",
publisher = "MDPI",
journal = "Biology",
title = "Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort",
volume = "11",
number = "10",
doi = "10.3390/biology11101437"
}

Dudašova Petrovičova, O., Stanković, I., Milinković, N., Dopsaj, V., Đorđević, B.,& Dopsaj, M.. (2022). Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort. in Biology
MDPI., 11(10).
https://doi.org/10.3390/biology11101437

Dudašova Petrovičova O, Stanković I, Milinković N, Dopsaj V, Đorđević B, Dopsaj M. Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort. in Biology. 2022;11(10).
doi:10.3390/biology11101437 .

Dudašova Petrovičova, Olina, Stanković, Ivan, Milinković, Neda, Dopsaj, Violeta, Đorđević, Brižita, Dopsaj, Milivoj, "Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort" in Biology, 11, no. 10 (2022),
https://doi.org/10.3390/biology11101437 . .

TY  - JOUR
AU  - Lalić-Ćosić, Sanja
AU  - Dopsaj, Violeta
AU  - Kovač, Mirjana
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Mobarrez, Fariborz
AU  - Antović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3997
AB  - Background: Pre-eclampsia (P-EC) is associated with systemic inflammation, endothelial dysfunction and hypercoagulability. The role of extracellular vesicles (EVs) in coagulation disturbances affecting the development and severity of P-EC remains elusive. We aimed to evaluate the concentration of EVs expressing phosphatidylserine (PS) and specific markers in relation to the thrombin and fibrin formation as well as fibrin clot properties, in pregnant women with P-EC in comparison to healthy pregnant women of similar gestational age. Methods: Blood samples of 30 pregnant women diagnosed with P-EC were collected on the morning following admission to hospital and after delivery (mean duration 5 days). The concentration of the PS-exposing EVs (PS+ EVs) from platelets (CD42a+, endothelial cells (CD62E+), and PS+ EVs expressing tissue factor (TF) and vascular cell adhesion molecule 1 (VCAM-1) were measured by flow cytometry. Further phenotyping of EVs also included expression of PlGF. Markers of maternal haemostasis were correlated with EVs concentration in plasma. Results: Preeclamptic pregnancy was associated with significantly higher plasma levels of PS+ CD42a+ EVs and PS+ VCAM-1+ EVs in comparison with normotensive pregnancy. P-EC patients after delivery had markedly elevated concentration of PS+ CD42a+ EVs, CD62E+ EVs, TF+ EVs, and VCAM-1+ EVs compared to those before delivery. Inverse correlation was observed between EVs concentrations (PS+, PS+ TF+, and PlGF+) and parameters of overall haemostatic potential (OHP) and fibrin formation, while PS+ VCAM-1+ EVs directly correlated with FVIII activity in plasma. Conclusion: Increased levels of PS+ EVs subpopulations in P-EC and their association with global haemostatic parameters, as well as with fibrin clot properties may suggest EVs involvement in intravascular fibrin deposition leading to subsequent microcirculation disorders.
PB  - Frontiers Media S.A.
T2  - Frontiers in Medicine
T1  - Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients
VL  - 8
DO  - 10.3389/fmed.2021.761453
ER  - 

@article{
author = "Lalić-Ćosić, Sanja and Dopsaj, Violeta and Kovač, Mirjana and Mandić-Marković, Vesna and Miković, Željko and Mobarrez, Fariborz and Antović, Aleksandra",
year = "2021",
abstract = "Background: Pre-eclampsia (P-EC) is associated with systemic inflammation, endothelial dysfunction and hypercoagulability. The role of extracellular vesicles (EVs) in coagulation disturbances affecting the development and severity of P-EC remains elusive. We aimed to evaluate the concentration of EVs expressing phosphatidylserine (PS) and specific markers in relation to the thrombin and fibrin formation as well as fibrin clot properties, in pregnant women with P-EC in comparison to healthy pregnant women of similar gestational age. Methods: Blood samples of 30 pregnant women diagnosed with P-EC were collected on the morning following admission to hospital and after delivery (mean duration 5 days). The concentration of the PS-exposing EVs (PS+ EVs) from platelets (CD42a+, endothelial cells (CD62E+), and PS+ EVs expressing tissue factor (TF) and vascular cell adhesion molecule 1 (VCAM-1) were measured by flow cytometry. Further phenotyping of EVs also included expression of PlGF. Markers of maternal haemostasis were correlated with EVs concentration in plasma. Results: Preeclamptic pregnancy was associated with significantly higher plasma levels of PS+ CD42a+ EVs and PS+ VCAM-1+ EVs in comparison with normotensive pregnancy. P-EC patients after delivery had markedly elevated concentration of PS+ CD42a+ EVs, CD62E+ EVs, TF+ EVs, and VCAM-1+ EVs compared to those before delivery. Inverse correlation was observed between EVs concentrations (PS+, PS+ TF+, and PlGF+) and parameters of overall haemostatic potential (OHP) and fibrin formation, while PS+ VCAM-1+ EVs directly correlated with FVIII activity in plasma. Conclusion: Increased levels of PS+ EVs subpopulations in P-EC and their association with global haemostatic parameters, as well as with fibrin clot properties may suggest EVs involvement in intravascular fibrin deposition leading to subsequent microcirculation disorders.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Medicine",
title = "Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients",
volume = "8",
doi = "10.3389/fmed.2021.761453"
}

Lalić-Ćosić, S., Dopsaj, V., Kovač, M., Mandić-Marković, V., Miković, Ž., Mobarrez, F.,& Antović, A.. (2021). Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients. in Frontiers in Medicine
Frontiers Media S.A.., 8.
https://doi.org/10.3389/fmed.2021.761453

Lalić-Ćosić S, Dopsaj V, Kovač M, Mandić-Marković V, Miković Ž, Mobarrez F, Antović A. Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients. in Frontiers in Medicine. 2021;8.
doi:10.3389/fmed.2021.761453 .

Lalić-Ćosić, Sanja, Dopsaj, Violeta, Kovač, Mirjana, Mandić-Marković, Vesna, Miković, Željko, Mobarrez, Fariborz, Antović, Aleksandra, "Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients" in Frontiers in Medicine, 8 (2021),
https://doi.org/10.3389/fmed.2021.761453 . .

TY  - JOUR
AU  - Savković, Miljan
AU  - Simić-Ogrizović, Sanja
AU  - Dopsaj, Violeta
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3903
AB  - Introduction: The aim of our study was to examine the relationship of hepcidin-25 with red blood cell and reticulocyte indices and to evaluate the diagnostic properties of hepcidin-25 in the assessment of positive iron balance in end-stage renal disease (ESRD) patients. Methods: Eighty anemic ESRD patients (hemoglobin < 110 g/L) were classified as having iron deficiency (ID, N = 20), iron sufficiency (IS, N = 29), and positive iron balance (PB, N = 31) using the conventional biomarkers for iron status evaluation. Hepcidin-25 was determined by a chemiluminescent direct ELISA. Results: Hepcidin-25 was significantly negatively correlated with the proportion of hypochromic erythrocytes (%HYPO) (P =.034) and immature reticulocyte fraction (P =.010) in ID and with the absolute reticulocyte concentration in ID (P =.048) and PB (P =.040). In multivariate models, hepcidin-25 was independently negatively associated with the mean reticulocyte hemoglobin content (CHr; β = −0.493, P =.004) and red blood cell size factor (RSf) (β = −0.334, P =.036) only in the PB group. The best hepcidin-25 value to exclude PB was 66.13 µg/L, showing a sensitivity of 61.3%, a specificity of 75.5%, and an AUC of 0.808. Conclusion: Our results suggest that hepcidin-25 levels are independently negatively associated with the iron demand for the most recent erythropoiesis only in PB. Hepcidin-25 performed acceptable in discriminating anemic ESRD patients with positive iron balance and may prove to be a useful additional tool in the evaluation of iron status.
PB  - John Wiley & Sons Ltd
T2  - International Journal of Laboratory Hematology
T1  - Assessment of positive iron balance in end-stage renal disease: Could hepcidin-25 be useful?
VL  - 43
IS  - 5
SP  - 1159
EP  - 1167
DO  - 10.1111/ijlh.13539
ER  - 

@article{
author = "Savković, Miljan and Simić-Ogrizović, Sanja and Dopsaj, Violeta",
year = "2021",
abstract = "Introduction: The aim of our study was to examine the relationship of hepcidin-25 with red blood cell and reticulocyte indices and to evaluate the diagnostic properties of hepcidin-25 in the assessment of positive iron balance in end-stage renal disease (ESRD) patients. Methods: Eighty anemic ESRD patients (hemoglobin < 110 g/L) were classified as having iron deficiency (ID, N = 20), iron sufficiency (IS, N = 29), and positive iron balance (PB, N = 31) using the conventional biomarkers for iron status evaluation. Hepcidin-25 was determined by a chemiluminescent direct ELISA. Results: Hepcidin-25 was significantly negatively correlated with the proportion of hypochromic erythrocytes (%HYPO) (P =.034) and immature reticulocyte fraction (P =.010) in ID and with the absolute reticulocyte concentration in ID (P =.048) and PB (P =.040). In multivariate models, hepcidin-25 was independently negatively associated with the mean reticulocyte hemoglobin content (CHr; β = −0.493, P =.004) and red blood cell size factor (RSf) (β = −0.334, P =.036) only in the PB group. The best hepcidin-25 value to exclude PB was 66.13 µg/L, showing a sensitivity of 61.3%, a specificity of 75.5%, and an AUC of 0.808. Conclusion: Our results suggest that hepcidin-25 levels are independently negatively associated with the iron demand for the most recent erythropoiesis only in PB. Hepcidin-25 performed acceptable in discriminating anemic ESRD patients with positive iron balance and may prove to be a useful additional tool in the evaluation of iron status.",
publisher = "John Wiley & Sons Ltd",
journal = "International Journal of Laboratory Hematology",
title = "Assessment of positive iron balance in end-stage renal disease: Could hepcidin-25 be useful?",
volume = "43",
number = "5",
pages = "1159-1167",
doi = "10.1111/ijlh.13539"
}

Savković, M., Simić-Ogrizović, S.,& Dopsaj, V.. (2021). Assessment of positive iron balance in end-stage renal disease: Could hepcidin-25 be useful?. in International Journal of Laboratory Hematology
John Wiley & Sons Ltd., 43(5), 1159-1167.
https://doi.org/10.1111/ijlh.13539

Savković M, Simić-Ogrizović S, Dopsaj V. Assessment of positive iron balance in end-stage renal disease: Could hepcidin-25 be useful?. in International Journal of Laboratory Hematology. 2021;43(5):1159-1167.
doi:10.1111/ijlh.13539 .

Savković, Miljan, Simić-Ogrizović, Sanja, Dopsaj, Violeta, "Assessment of positive iron balance in end-stage renal disease: Could hepcidin-25 be useful?" in International Journal of Laboratory Hematology, 43, no. 5 (2021):1159-1167,
https://doi.org/10.1111/ijlh.13539 . .

TY  - JOUR
AU  - Savković, Miljan
AU  - Simić-Ogrizović, Sanja
AU  - Dopsaj, Violeta
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3778
AB  - This study aimed to investigate the factors that are independently associated with hepcidin-25 and its relationship with doses of erythropoiesis-stimulating agents (ESAs) and intravenous iron in stable maintenance hemodialysis patients (smHD) stratified by ESAs administration. In 103 adult smHD (ESAs therapy (N = 64) and ESAs-free (N = 39)), median values of biologically active hepcidin-25 (chemiluminescent direct ELISA assay) and ferritin levels were significantly higher whereas red blood cell count, hemoglobin, and hematocrit values were lower in ESAs therapy compared to ESAs-free group (P <.001, for all). Our results suggest that ESAs-independent smHD exhibit supposedly normal hepcidin-25 levels and preserved iron homeostasis, with a lower degree of anemia. The results of our multivariable model indicate that hepcidin-25 levels are independently and positively associated with iron stores and inflammation, and inversely with active erythropoiesis, regardless of ESAs administration. Maintenance ESAs and the intravenous iron dose were not related to hepcidin-25 levels.
PB  - Blackwell Publishing Ltd
T2  - Therapeutic Apheresis and Dialysis
T1  - Factors associated with hepcidin-25 levels in maintenance hemodialysis patients
DO  - 10.1111/1744-9987.13617
ER  - 

@article{
author = "Savković, Miljan and Simić-Ogrizović, Sanja and Dopsaj, Violeta",
year = "2021",
abstract = "This study aimed to investigate the factors that are independently associated with hepcidin-25 and its relationship with doses of erythropoiesis-stimulating agents (ESAs) and intravenous iron in stable maintenance hemodialysis patients (smHD) stratified by ESAs administration. In 103 adult smHD (ESAs therapy (N = 64) and ESAs-free (N = 39)), median values of biologically active hepcidin-25 (chemiluminescent direct ELISA assay) and ferritin levels were significantly higher whereas red blood cell count, hemoglobin, and hematocrit values were lower in ESAs therapy compared to ESAs-free group (P <.001, for all). Our results suggest that ESAs-independent smHD exhibit supposedly normal hepcidin-25 levels and preserved iron homeostasis, with a lower degree of anemia. The results of our multivariable model indicate that hepcidin-25 levels are independently and positively associated with iron stores and inflammation, and inversely with active erythropoiesis, regardless of ESAs administration. Maintenance ESAs and the intravenous iron dose were not related to hepcidin-25 levels.",
publisher = "Blackwell Publishing Ltd",
journal = "Therapeutic Apheresis and Dialysis",
title = "Factors associated with hepcidin-25 levels in maintenance hemodialysis patients",
doi = "10.1111/1744-9987.13617"
}

Savković, M., Simić-Ogrizović, S.,& Dopsaj, V.. (2021). Factors associated with hepcidin-25 levels in maintenance hemodialysis patients. in Therapeutic Apheresis and Dialysis
Blackwell Publishing Ltd..
https://doi.org/10.1111/1744-9987.13617

Savković M, Simić-Ogrizović S, Dopsaj V. Factors associated with hepcidin-25 levels in maintenance hemodialysis patients. in Therapeutic Apheresis and Dialysis. 2021;.
doi:10.1111/1744-9987.13617 .

Savković, Miljan, Simić-Ogrizović, Sanja, Dopsaj, Violeta, "Factors associated with hepcidin-25 levels in maintenance hemodialysis patients" in Therapeutic Apheresis and Dialysis (2021),
https://doi.org/10.1111/1744-9987.13617 . .

TY  - JOUR
AU  - Dopsaj, Milivoj
AU  - Kukić, Filip
AU  - Ðordević-Nikić, Marina
AU  - Koropanovski, Nenad
AU  - Radovanović, Dragan
AU  - Miljuš, Dragan
AU  - Subošić, Dane
AU  - Tomanić, Milena
AU  - Dopsaj, Violeta
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3649
AB  - This study aimed to explore the set of variables related to skeletal muscle mass (SMM) inboth sexes, and to create age- and sex-related models of changes in SMM, using the most representativeindicator of muscular status. Body composition was assessed in 8733 subjects (♀=3370 and♂=5363),allocated into subsamples according to age: 18–29.9, 30–39.9, 40–49.9, 50–59.9, 60–69.9, and 70.0–79.9years. Nine variables were used: protein mass, protein percent, protein mass index, SMM, percentof SMM, SMM index,  fat-free mass,  fat-free mass index,  and protein/fat index.   Univariate andmultivariate analysis of variance (ANOVA and MANOVA) were used to determine between- andwithin-sex difference in all variables by age. Correlation analysis established the relationship betweenage and muscularity variables. Principal Component Analysis extracted the variables that loadedhighest in explaining muscularity, while regression analysis determined the linearity of associationbetween the age and indicators of muscular status. Variables SMMI and PSMM were extracted asthe most sensitive to age, with SMMI being gender-independent while showing the parabolic andsinusoidal form of change as function of ageing in males and females, respectively; and PSMM beingsex-dependent while showing a linear trend of decrease in both sexes.
PB  - MDPI AG
T2  - International Journal of Environmental Research and Public Health
T1  - Indicators of absolute and relative changes in skeletal muscle mass during adulthood and ageing
VL  - 17
IS  - 16
SP  - 1
EP  - 13
DO  - 10.3390/ijerph17165977
ER  - 

@article{
author = "Dopsaj, Milivoj and Kukić, Filip and Ðordević-Nikić, Marina and Koropanovski, Nenad and Radovanović, Dragan and Miljuš, Dragan and Subošić, Dane and Tomanić, Milena and Dopsaj, Violeta",
year = "2020",
abstract = "This study aimed to explore the set of variables related to skeletal muscle mass (SMM) inboth sexes, and to create age- and sex-related models of changes in SMM, using the most representativeindicator of muscular status. Body composition was assessed in 8733 subjects (♀=3370 and♂=5363),allocated into subsamples according to age: 18–29.9, 30–39.9, 40–49.9, 50–59.9, 60–69.9, and 70.0–79.9years. Nine variables were used: protein mass, protein percent, protein mass index, SMM, percentof SMM, SMM index,  fat-free mass,  fat-free mass index,  and protein/fat index.   Univariate andmultivariate analysis of variance (ANOVA and MANOVA) were used to determine between- andwithin-sex difference in all variables by age. Correlation analysis established the relationship betweenage and muscularity variables. Principal Component Analysis extracted the variables that loadedhighest in explaining muscularity, while regression analysis determined the linearity of associationbetween the age and indicators of muscular status. Variables SMMI and PSMM were extracted asthe most sensitive to age, with SMMI being gender-independent while showing the parabolic andsinusoidal form of change as function of ageing in males and females, respectively; and PSMM beingsex-dependent while showing a linear trend of decrease in both sexes.",
publisher = "MDPI AG",
journal = "International Journal of Environmental Research and Public Health",
title = "Indicators of absolute and relative changes in skeletal muscle mass during adulthood and ageing",
volume = "17",
number = "16",
pages = "1-13",
doi = "10.3390/ijerph17165977"
}

Dopsaj, M., Kukić, F., Ðordević-Nikić, M., Koropanovski, N., Radovanović, D., Miljuš, D., Subošić, D., Tomanić, M.,& Dopsaj, V.. (2020). Indicators of absolute and relative changes in skeletal muscle mass during adulthood and ageing. in International Journal of Environmental Research and Public Health
MDPI AG., 17(16), 1-13.
https://doi.org/10.3390/ijerph17165977

Dopsaj M, Kukić F, Ðordević-Nikić M, Koropanovski N, Radovanović D, Miljuš D, Subošić D, Tomanić M, Dopsaj V. Indicators of absolute and relative changes in skeletal muscle mass during adulthood and ageing. in International Journal of Environmental Research and Public Health. 2020;17(16):1-13.
doi:10.3390/ijerph17165977 .

Dopsaj, Milivoj, Kukić, Filip, Ðordević-Nikić, Marina, Koropanovski, Nenad, Radovanović, Dragan, Miljuš, Dragan, Subošić, Dane, Tomanić, Milena, Dopsaj, Violeta, "Indicators of absolute and relative changes in skeletal muscle mass during adulthood and ageing" in International Journal of Environmental Research and Public Health, 17, no. 16 (2020):1-13,
https://doi.org/10.3390/ijerph17165977 . .

TY  - JOUR
AU  - Kukić, Filip
AU  - Dopsaj, Milivoj
AU  - Đorđević Nikić, Marina
AU  - Koropanovski, Nenad
AU  - Maksimović, Miloš
AU  - Eminović, Fadilj
AU  - Dopsaj, Violeta
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3619
PB  - Iranian Public Health Association
T2  - Iranian Journal of Public Health
T1  - Educational status, leisure-time physical activity and body composition in serbian adult population
VL  - 49
IS  - 3
SP  - 606
EP  - 608
UR  - https://hdl.handle.net/21.15107/rcub_rfasper_1290
ER  - 

@article{
author = "Kukić, Filip and Dopsaj, Milivoj and Đorđević Nikić, Marina and Koropanovski, Nenad and Maksimović, Miloš and Eminović, Fadilj and Dopsaj, Violeta",
year = "2020",
publisher = "Iranian Public Health Association",
journal = "Iranian Journal of Public Health",
title = "Educational status, leisure-time physical activity and body composition in serbian adult population",
volume = "49",
number = "3",
pages = "606-608",
url = "https://hdl.handle.net/21.15107/rcub_rfasper_1290"
}

Kukić, F., Dopsaj, M., Đorđević Nikić, M., Koropanovski, N., Maksimović, M., Eminović, F.,& Dopsaj, V.. (2020). Educational status, leisure-time physical activity and body composition in serbian adult population. in Iranian Journal of Public Health
Iranian Public Health Association., 49(3), 606-608.
https://hdl.handle.net/21.15107/rcub_rfasper_1290

Kukić F, Dopsaj M, Đorđević Nikić M, Koropanovski N, Maksimović M, Eminović F, Dopsaj V. Educational status, leisure-time physical activity and body composition in serbian adult population. in Iranian Journal of Public Health. 2020;49(3):606-608.
https://hdl.handle.net/21.15107/rcub_rfasper_1290 .

Kukić, Filip, Dopsaj, Milivoj, Đorđević Nikić, Marina, Koropanovski, Nenad, Maksimović, Miloš, Eminović, Fadilj, Dopsaj, Violeta, "Educational status, leisure-time physical activity and body composition in serbian adult population" in Iranian Journal of Public Health, 49, no. 3 (2020):606-608,
https://hdl.handle.net/21.15107/rcub_rfasper_1290 .

TY  - JOUR
AU  - Ćulafić, Milica
AU  - Vezmar-Kovačević, Sandra
AU  - Dopsaj, Violeta
AU  - Oluić, Branislav
AU  - Bidžić, Nemanja
AU  - Miljković, Branislava
AU  - Ćulafić, Đorđe
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3625
AB  - Background:The progression of the nonalcoholic fatty liver disease to nonalcoholic steatohepatitis (NASH) is multifactorial, and there is still a lack of approved medications for its  treatment.  The  study  aimed  to  evaluate  the  impact  of combined treatment with Pentoxifylline and Metformin on biochemical  parameters  in  patients  with  NASH.  Setting:Outpatient hepatology clinic.Methods:A  prospective  trial  was  conducted.  The  first cohort  included  patients  with  biopsy-proven  NASH,  while the  second  cohort  consisted  of  patients  with  biopsy-confirmed NAFLD. Blood tests were checked at baseline and every three months. Pentoxifylline at a dosage of 400 mgt.i.d.  and  Metformin  at  the  dosage  of  500  mg  t.i.d.  were introduced for six months in NASH group.  The impact of the  treatment  was  assessed  based  on  biochemical  results after combined treatment with low-cost medications. Results:All  33  NASH  patients  completed  24  weeks  of treatment. We observed significant improvement (p<0.05)of median values after treatment for the following parameters: serum uric acid levels decreased by 51.0 mmol/L, calcium decreased for 0.27 mmoL/L, magnesium showed an increase of 0.11 mmoL/L. Insulin resistance improved as a reduction of HOMA – IR by 1.3 was detected. A significant decrease of median in liver enzymes, alanine aminotransferase,  aspartate  aminotransferase  and  gamma-glutamyltransferase  by  24.0  U/L,  9.1  U/L,  10.8  U/L  respectively,was noted. Conclusions:Pentoxifylline  and  Metformin  may  provide possible  treatment  option  in  NASH.  Some  new  potential benefit  of  the  therapy  in  improving  liver  function  whilst decreasing cardiovascular risk was perceived.
AB  - Uvod: Progresija nealkoholne masne bolesti jetre (NAFLD) do nealkoholnog steatohepatitisa (NASH) je multifaktorijalna, i još uvek ne postoje zvanično odobreni lekovi za terapiju. U studiji je procenjivan uticaj kombinovane terapije pentoksifilinom i metforminom na biohemijske parametre kod pacijenata sa NASHom. Okruženje: Hepatološka ambulanta klinike. Metode: Studija je bila prospektivnog karaktera. Prva grupa uključila je pacijente sa biopsijom potvrđenim NASHom, dok su drugu grupu činili pacijenti sa biopsijom potvrđenom NAFLDom. Analize krvi vršene su svaka tri meseca. Uvedena je kombinovana terapija pentoksifilinom u dozi od 400 mg 3×1 i metforminom u dozi od 500 mg 3×1, u trajanju od šest meseci za Nash grupu. Efekat lečenja procenjen je na osnovu biohemijskih parametara nakon terapije ovim medikamentima sa niskom cenom. Rezultati: Svih 33 pacijenta su završila 24 nedelje terapije. Uočeno je značajno poboljšanje (p<0,05) vrednosti medijane za sledeće parametre: nivoi mokraćne kiseline u serumu su se snizili za 51,0 mmol/L, kalcijum je snižen za 0,27 mmol/L, magnezijum je pokazao porast za 0,11 mmol/L. Poboljšana je insulinska rezistencija konstatovana kroz smanjenje HOMA-IR za 1,3. Zabeleženo je značajno smanjenje medijane enzima jetre: alanin aminotransferaze za 24,0 U/L, aspartat aminotransferaze za 9,1 U/L i gama glutamiltransferaze za 10,8 U/L. Zaključak: Pentoksifilin sa metforminom može predstavljati moguću terapijsku opciju u NASHu. Primećena je nova potencijalna korist primenjene terapije u poboljšanju funkcije jetre i smanjenju kardiovaskularnog rizika.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis
T1  - Terapija pentoksifilinom sa metforminom poboljšava biohemijske parametre u pacijenata sa nealkoholnim steatohepatitisom
VL  - 39
IS  - 3
SP  - 290
EP  - 298
DO  - 10.2478/jomb-2019-0043
ER  - 

@article{
author = "Ćulafić, Milica and Vezmar-Kovačević, Sandra and Dopsaj, Violeta and Oluić, Branislav and Bidžić, Nemanja and Miljković, Branislava and Ćulafić, Đorđe",
year = "2020",
abstract = "Background:The progression of the nonalcoholic fatty liver disease to nonalcoholic steatohepatitis (NASH) is multifactorial, and there is still a lack of approved medications for its  treatment.  The  study  aimed  to  evaluate  the  impact  of combined treatment with Pentoxifylline and Metformin on biochemical  parameters  in  patients  with  NASH.  Setting:Outpatient hepatology clinic.Methods:A  prospective  trial  was  conducted.  The  first cohort  included  patients  with  biopsy-proven  NASH,  while the  second  cohort  consisted  of  patients  with  biopsy-confirmed NAFLD. Blood tests were checked at baseline and every three months. Pentoxifylline at a dosage of 400 mgt.i.d.  and  Metformin  at  the  dosage  of  500  mg  t.i.d.  were introduced for six months in NASH group.  The impact of the  treatment  was  assessed  based  on  biochemical  results after combined treatment with low-cost medications. Results:All  33  NASH  patients  completed  24  weeks  of treatment. We observed significant improvement (p<0.05)of median values after treatment for the following parameters: serum uric acid levels decreased by 51.0 mmol/L, calcium decreased for 0.27 mmoL/L, magnesium showed an increase of 0.11 mmoL/L. Insulin resistance improved as a reduction of HOMA – IR by 1.3 was detected. A significant decrease of median in liver enzymes, alanine aminotransferase,  aspartate  aminotransferase  and  gamma-glutamyltransferase  by  24.0  U/L,  9.1  U/L,  10.8  U/L  respectively,was noted. Conclusions:Pentoxifylline  and  Metformin  may  provide possible  treatment  option  in  NASH.  Some  new  potential benefit  of  the  therapy  in  improving  liver  function  whilst decreasing cardiovascular risk was perceived., Uvod: Progresija nealkoholne masne bolesti jetre (NAFLD) do nealkoholnog steatohepatitisa (NASH) je multifaktorijalna, i još uvek ne postoje zvanično odobreni lekovi za terapiju. U studiji je procenjivan uticaj kombinovane terapije pentoksifilinom i metforminom na biohemijske parametre kod pacijenata sa NASHom. Okruženje: Hepatološka ambulanta klinike. Metode: Studija je bila prospektivnog karaktera. Prva grupa uključila je pacijente sa biopsijom potvrđenim NASHom, dok su drugu grupu činili pacijenti sa biopsijom potvrđenom NAFLDom. Analize krvi vršene su svaka tri meseca. Uvedena je kombinovana terapija pentoksifilinom u dozi od 400 mg 3×1 i metforminom u dozi od 500 mg 3×1, u trajanju od šest meseci za Nash grupu. Efekat lečenja procenjen je na osnovu biohemijskih parametara nakon terapije ovim medikamentima sa niskom cenom. Rezultati: Svih 33 pacijenta su završila 24 nedelje terapije. Uočeno je značajno poboljšanje (p<0,05) vrednosti medijane za sledeće parametre: nivoi mokraćne kiseline u serumu su se snizili za 51,0 mmol/L, kalcijum je snižen za 0,27 mmol/L, magnezijum je pokazao porast za 0,11 mmol/L. Poboljšana je insulinska rezistencija konstatovana kroz smanjenje HOMA-IR za 1,3. Zabeleženo je značajno smanjenje medijane enzima jetre: alanin aminotransferaze za 24,0 U/L, aspartat aminotransferaze za 9,1 U/L i gama glutamiltransferaze za 10,8 U/L. Zaključak: Pentoksifilin sa metforminom može predstavljati moguću terapijsku opciju u NASHu. Primećena je nova potencijalna korist primenjene terapije u poboljšanju funkcije jetre i smanjenju kardiovaskularnog rizika.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis, Terapija pentoksifilinom sa metforminom poboljšava biohemijske parametre u pacijenata sa nealkoholnim steatohepatitisom",
volume = "39",
number = "3",
pages = "290-298",
doi = "10.2478/jomb-2019-0043"
}

Ćulafić, M., Vezmar-Kovačević, S., Dopsaj, V., Oluić, B., Bidžić, N., Miljković, B.,& Ćulafić, Đ.. (2020). Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 290-298.
https://doi.org/10.2478/jomb-2019-0043

Ćulafić M, Vezmar-Kovačević S, Dopsaj V, Oluić B, Bidžić N, Miljković B, Ćulafić Đ. Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis. in Journal of Medical Biochemistry. 2020;39(3):290-298.
doi:10.2478/jomb-2019-0043 .

Ćulafić, Milica, Vezmar-Kovačević, Sandra, Dopsaj, Violeta, Oluić, Branislav, Bidžić, Nemanja, Miljković, Branislava, Ćulafić, Đorđe, "Pentoxifylline with metformin treatment improves biochemical parameters in patients with nonalcoholic steatohepatitis" in Journal of Medical Biochemistry, 39, no. 3 (2020):290-298,
https://doi.org/10.2478/jomb-2019-0043 . .

TY  - JOUR
AU  - Lalić-Ćosić, Sanja
AU  - Dopsaj, Violeta
AU  - Kovač, Mirjana
AU  - Pruner, Iva
AU  - Littmann, Karin
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Antović, Aleksandra
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3609
AB  - Introduction: Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods: Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays—endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)—were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results: Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP—determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion: Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.
PB  - Wiley-Blackwell
T2  - International Journal of Laboratory Hematology
T1  - Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia
VL  - 42
IS  - 3
SP  - 322
EP  - 330
DO  - 10.1111/ijlh.13183
ER  - 

@article{
author = "Lalić-Ćosić, Sanja and Dopsaj, Violeta and Kovač, Mirjana and Pruner, Iva and Littmann, Karin and Mandić-Marković, Vesna and Miković, Željko and Antović, Aleksandra",
year = "2020",
abstract = "Introduction: Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods: Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays—endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)—were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results: Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP—determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion: Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.",
publisher = "Wiley-Blackwell",
journal = "International Journal of Laboratory Hematology",
title = "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia",
volume = "42",
number = "3",
pages = "322-330",
doi = "10.1111/ijlh.13183"
}

Lalić-Ćosić, S., Dopsaj, V., Kovač, M., Pruner, I., Littmann, K., Mandić-Marković, V., Miković, Ž.,& Antović, A.. (2020). Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology
Wiley-Blackwell., 42(3), 322-330.
https://doi.org/10.1111/ijlh.13183

Lalić-Ćosić S, Dopsaj V, Kovač M, Pruner I, Littmann K, Mandić-Marković V, Miković Ž, Antović A. Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology. 2020;42(3):322-330.
doi:10.1111/ijlh.13183 .

Lalić-Ćosić, Sanja, Dopsaj, Violeta, Kovač, Mirjana, Pruner, Iva, Littmann, Karin, Mandić-Marković, Vesna, Miković, Željko, Antović, Aleksandra, "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia" in International Journal of Laboratory Hematology, 42, no. 3 (2020):322-330,
https://doi.org/10.1111/ijlh.13183 . .

TY  - JOUR
AU  - Dopsaj, Violeta
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3740
AB  - The classical oral anticoagulants are increasingly being replaced in clinical practice by new antithrombotic drugs, which act by enabling direct inhibition of coagulation factor IIa (FIIa) or factor Xa (FXa). These drugs have multiple acronyms, including NOACs (new, non–vitamin K antagonist)  or  DOACs  (direct  oral  anticoagulants),  and  currently  include  dabigatran  (FIIa inhibitor), and rivaroxaban, apixaban, and edoxaban (FXa inhibitors). These drugs are approved for stroke prevention in patients with non-valvular atrial fibrillation and the prevention and treatment of venous thromboembolism. The "mantra" that DOACs do not require laboratory monitoring is not entirely correct because laboratory testing for drug effects is needed in many situations, because they influence hemostasis tests and in situations in which urgent measurement of DOACs is required. This should be very important to consider in the clinical situation for numbers of indications and increasing numbers of patients on DOACs therapy. The main aim of this article is to provide practical issues to general laboratory testing for DOACs, as well as to help  avoid  diagnostic  errors  associated  with  hemostasis  testing.  The  assays  for  DOAC quantification must be available in medical centers on a whole day basis, to facilitate optimal drug management in conditions when things go wrong or in urgent cases of immediate reversal of anticoagulation or appropriate administration of a specific antidote.
AB  - Nova generacija oralnih antikoagulantnih lekova omogućava direktnu inhibiciju FIIa ili FXa, i sve više zamenjuje klasične antikoagulanse u kliničkoj praksi za različita stanja. Ovi lekovi su označeni sa nekoliko akronima, uključujući NOAC, DOAC, i odnose se na nove (ne-vitamin K antagoniste) i direktne oralne antikoagulanse, a trenutno uključuju dabigatran (FIIa inhibitor), i rivaroksaban, apiksaban i edoksaban (FXa inhibitori) . Direktni oralni antikoagulantni lekovi odobreni su za prevenciju moždanog udara kod pacijenata sa ne-valvularnom atrijalnom fibrilacijom i za prevenciju i lečenje venskog tromboembolizma. "Mantra" da direktni oralni antikoagulantni lekovi ne zahtevaju laboratorijsko praćenje ne može se prihvatiti u mnogim kliničkim situacijama. Štaviše, pošto ovi lekovi "ne zahtevaju" laboratorijsko praćenje, neki kliničari su to prihvatili kao da ne utiču na testove u hemostazi. Postoji više situacija u kojima je potrebno rutinsko i hitno određivanje DOAC-a, pri čemu će se broj laboratorijskih zahteva povećavati u budućnosti jer sve više zamenjuju konvencionalne antikoagulanse za sve veći broj indikacija, kod sve većeg broja pacijenata. Glavni cilj ovog rada je da odgovori na praktična pitanja u opštem laboratorijskom ispitivanju DOAC-a, kao i da pomogne u uklanjanju i smanjenju dijagnostičkih grešaka povezanih sa ispitivanjem hemostaze. Testovi za kvantifikaciju DOAC-a moraju biti dostupni u medicinskim centrima 24 sata, kako bi se olakšalo optimalno lečenje u uslovima kada stvari pođu po zlu ili u hitnim slučajevima trenutnog ukidanja antikoagulantne terapije ili odgovarajuće primene specifičnog antidota.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Practical issues in measuring the anticoagulant effect of direct oral anticoagulants
T1  - Praktični aspekti u određivanju antikoagulantnog dejstva direktnih oralnih antikoagulantnih lekova
VL  - 70
IS  - 5
SP  - 297
EP  - 309
DO  - 10.5937/arhfarm2005297D
ER  - 

@article{
author = "Dopsaj, Violeta",
year = "2020",
abstract = "The classical oral anticoagulants are increasingly being replaced in clinical practice by new antithrombotic drugs, which act by enabling direct inhibition of coagulation factor IIa (FIIa) or factor Xa (FXa). These drugs have multiple acronyms, including NOACs (new, non–vitamin K antagonist)  or  DOACs  (direct  oral  anticoagulants),  and  currently  include  dabigatran  (FIIa inhibitor), and rivaroxaban, apixaban, and edoxaban (FXa inhibitors). These drugs are approved for stroke prevention in patients with non-valvular atrial fibrillation and the prevention and treatment of venous thromboembolism. The "mantra" that DOACs do not require laboratory monitoring is not entirely correct because laboratory testing for drug effects is needed in many situations, because they influence hemostasis tests and in situations in which urgent measurement of DOACs is required. This should be very important to consider in the clinical situation for numbers of indications and increasing numbers of patients on DOACs therapy. The main aim of this article is to provide practical issues to general laboratory testing for DOACs, as well as to help  avoid  diagnostic  errors  associated  with  hemostasis  testing.  The  assays  for  DOAC quantification must be available in medical centers on a whole day basis, to facilitate optimal drug management in conditions when things go wrong or in urgent cases of immediate reversal of anticoagulation or appropriate administration of a specific antidote., Nova generacija oralnih antikoagulantnih lekova omogućava direktnu inhibiciju FIIa ili FXa, i sve više zamenjuje klasične antikoagulanse u kliničkoj praksi za različita stanja. Ovi lekovi su označeni sa nekoliko akronima, uključujući NOAC, DOAC, i odnose se na nove (ne-vitamin K antagoniste) i direktne oralne antikoagulanse, a trenutno uključuju dabigatran (FIIa inhibitor), i rivaroksaban, apiksaban i edoksaban (FXa inhibitori) . Direktni oralni antikoagulantni lekovi odobreni su za prevenciju moždanog udara kod pacijenata sa ne-valvularnom atrijalnom fibrilacijom i za prevenciju i lečenje venskog tromboembolizma. "Mantra" da direktni oralni antikoagulantni lekovi ne zahtevaju laboratorijsko praćenje ne može se prihvatiti u mnogim kliničkim situacijama. Štaviše, pošto ovi lekovi "ne zahtevaju" laboratorijsko praćenje, neki kliničari su to prihvatili kao da ne utiču na testove u hemostazi. Postoji više situacija u kojima je potrebno rutinsko i hitno određivanje DOAC-a, pri čemu će se broj laboratorijskih zahteva povećavati u budućnosti jer sve više zamenjuju konvencionalne antikoagulanse za sve veći broj indikacija, kod sve većeg broja pacijenata. Glavni cilj ovog rada je da odgovori na praktična pitanja u opštem laboratorijskom ispitivanju DOAC-a, kao i da pomogne u uklanjanju i smanjenju dijagnostičkih grešaka povezanih sa ispitivanjem hemostaze. Testovi za kvantifikaciju DOAC-a moraju biti dostupni u medicinskim centrima 24 sata, kako bi se olakšalo optimalno lečenje u uslovima kada stvari pođu po zlu ili u hitnim slučajevima trenutnog ukidanja antikoagulantne terapije ili odgovarajuće primene specifičnog antidota.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Practical issues in measuring the anticoagulant effect of direct oral anticoagulants, Praktični aspekti u određivanju antikoagulantnog dejstva direktnih oralnih antikoagulantnih lekova",
volume = "70",
number = "5",
pages = "297-309",
doi = "10.5937/arhfarm2005297D"
}

Dopsaj, V.. (2020). Practical issues in measuring the anticoagulant effect of direct oral anticoagulants. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 70(5), 297-309.
https://doi.org/10.5937/arhfarm2005297D

Dopsaj V. Practical issues in measuring the anticoagulant effect of direct oral anticoagulants. in Arhiv za farmaciju. 2020;70(5):297-309.
doi:10.5937/arhfarm2005297D .

Dopsaj, Violeta, "Practical issues in measuring the anticoagulant effect of direct oral anticoagulants" in Arhiv za farmaciju, 70, no. 5 (2020):297-309,
https://doi.org/10.5937/arhfarm2005297D . .

TY  - JOUR
AU  - Dopsaj, Milivoj
AU  - Nenasheva, A.V
AU  - Tretiakova, T.N
AU  - Syromiatnikova, Yu.A
AU  - Surina-Marysheva, E.F
AU  - Marković, S
AU  - Dopsaj, Violeta
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3322
AB  - The Aim. The aim of this paper is to define the contractile characteristics of maximal isometric handgrip force and to establish possible differences between the students of the Universities of Chelyabinsk and Belgrade. The secondary aim is to create a practically applicable initial model in order to explore the mentioned characteristics in the future. Material and methods. For the purposes of this research, the overall sample of 225 subjects was tested. 91 subjects were from Russia and 134 were from Serbia. The results were gathered using a strain gage and a standardized isometric handgrip test protocol. The contractile properties of HG muscle force were measured in relation to three different contractile dimensions: the maximal muscle force (Fmax), the maximal explosive muscle force (RFDmax), the time need for achieving maximal (tFmax) and maximal explosive (tRFDmax) muscle force. Results. This study has shown that there are no generally statistically significant differences for all observed variables of HG contractile characteristics between tested Russian and Serbian students. Based on the results of the present study, six different models of the equation for evaluation of HG contractile characteristics of female and male students, i.e. young adults, were made. All defined models are highly statistically significant, accurate and sensitive in the prediction of the general distributive position of an individual or particular group of subjects in relation to the measured contractile characteristics. Conclusions. The obtained results can generally indicate the stability of potential to exert the given contractile characteristic in relation to the population of similar evolutionary (Slavs) at different geographical background.
PB  - South Ural State University - Institute of Sport, Tourism and Service
T2  - Human Sport Medicine
T1  - Handgrip muscle force characteristics with general reference values at Chelyabinsk and Belgrade students
VL  - 19
IS  - 2
SP  - 27
EP  - 36
DO  - 10.14529/hsm190204
ER  - 

@article{
author = "Dopsaj, Milivoj and Nenasheva, A.V and Tretiakova, T.N and Syromiatnikova, Yu.A and Surina-Marysheva, E.F and Marković, S and Dopsaj, Violeta",
year = "2019",
abstract = "The Aim. The aim of this paper is to define the contractile characteristics of maximal isometric handgrip force and to establish possible differences between the students of the Universities of Chelyabinsk and Belgrade. The secondary aim is to create a practically applicable initial model in order to explore the mentioned characteristics in the future. Material and methods. For the purposes of this research, the overall sample of 225 subjects was tested. 91 subjects were from Russia and 134 were from Serbia. The results were gathered using a strain gage and a standardized isometric handgrip test protocol. The contractile properties of HG muscle force were measured in relation to three different contractile dimensions: the maximal muscle force (Fmax), the maximal explosive muscle force (RFDmax), the time need for achieving maximal (tFmax) and maximal explosive (tRFDmax) muscle force. Results. This study has shown that there are no generally statistically significant differences for all observed variables of HG contractile characteristics between tested Russian and Serbian students. Based on the results of the present study, six different models of the equation for evaluation of HG contractile characteristics of female and male students, i.e. young adults, were made. All defined models are highly statistically significant, accurate and sensitive in the prediction of the general distributive position of an individual or particular group of subjects in relation to the measured contractile characteristics. Conclusions. The obtained results can generally indicate the stability of potential to exert the given contractile characteristic in relation to the population of similar evolutionary (Slavs) at different geographical background.",
publisher = "South Ural State University - Institute of Sport, Tourism and Service",
journal = "Human Sport Medicine",
title = "Handgrip muscle force characteristics with general reference values at Chelyabinsk and Belgrade students",
volume = "19",
number = "2",
pages = "27-36",
doi = "10.14529/hsm190204"
}

Dopsaj, M., Nenasheva, A.V, Tretiakova, T.N, Syromiatnikova, Yu.A, Surina-Marysheva, E.F, Marković, S.,& Dopsaj, V.. (2019). Handgrip muscle force characteristics with general reference values at Chelyabinsk and Belgrade students. in Human Sport Medicine
South Ural State University - Institute of Sport, Tourism and Service., 19(2), 27-36.
https://doi.org/10.14529/hsm190204

Dopsaj M, Nenasheva A, Tretiakova T, Syromiatnikova Y, Surina-Marysheva E, Marković S, Dopsaj V. Handgrip muscle force characteristics with general reference values at Chelyabinsk and Belgrade students. in Human Sport Medicine. 2019;19(2):27-36.
doi:10.14529/hsm190204 .

Dopsaj, Milivoj, Nenasheva, A.V, Tretiakova, T.N, Syromiatnikova, Yu.A, Surina-Marysheva, E.F, Marković, S, Dopsaj, Violeta, "Handgrip muscle force characteristics with general reference values at Chelyabinsk and Belgrade students" in Human Sport Medicine, 19, no. 2 (2019):27-36,
https://doi.org/10.14529/hsm190204 . .

TY  - JOUR
AU  - Ćulafić, Milica
AU  - Vezmar-Kovačević, Sandra
AU  - Dopsaj, Violeta
AU  - Stulić, Miloš
AU  - Vlaisavljević, Željko
AU  - Miljković, Branislava
AU  - Ćulafić, Đorđe
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3262
AB  - Background and objectives: Data suggests that nearly 30% of the general population have steatosis and up to 5% of this population develops nonalcoholic steatohepatitis (NASH). Liver biopsy is still considered to be the gold standard for the diagnosis of NASH. Great effort is being made toward the identification of sensitive diagnostic tests that do not involve invasive procedures to address a common concern in patients with the nonalcoholic fatty liver disease-whether they have NASH or simple steatosis. We aimed to investigate the independent predictors and develop a non-invasive, easy-to-perform, low-cost set of parameters that may be used in clinical practice to differentiate simple steatosis from NASH. Methods: А cross-sectional study of nonalcoholic fatty liver disease (NAFLD) patients divided into two groups: group I-simple steatosis (SS) and group II-biopsy-proven NASH. Strict inclusion criteria and stepwise analysis allowed the evaluation of a vast number of measured/estimated parameters. Results: One hundred and eleven patients were included-82 with simple steatosis and 29 with biopsy-proven NASH. The probability of NASH was the highest when homeostatic model assessment of insulin resistance (HOMA-IR) was above 2.5, uric acid above 380 µmol/L, ferritin above 100 µg/L and ALT above 45 U/L. An acronym of using first letters was created and named the HUFA index. This combined model resulted in an area under the receiver operator characteristic curve (AUROC) of 0.94, provided sensitivity, specificity, positive predictive value and a negative predictive value for NASH of 70.3%, 95.1%, 83.1% and 90.0%, respectively. Conclusion: We suggest a simple non-invasive predictive index HUFA that encompasses four easily available parameters (HOMA-IR, uric acid, ferritin and ALT) to identify patients with NASH, which may reduce the need for a liver biopsy on a routine basis in patients with NAFLD.
PB  - NLM (Medline)
T2  - Medicina (Kaunas, Lithuania)
T1  - A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests
VL  - 55
IS  - 6
DO  - 10.3390/medicina55060243
ER  - 

@article{
author = "Ćulafić, Milica and Vezmar-Kovačević, Sandra and Dopsaj, Violeta and Stulić, Miloš and Vlaisavljević, Željko and Miljković, Branislava and Ćulafić, Đorđe",
year = "2019",
abstract = "Background and objectives: Data suggests that nearly 30% of the general population have steatosis and up to 5% of this population develops nonalcoholic steatohepatitis (NASH). Liver biopsy is still considered to be the gold standard for the diagnosis of NASH. Great effort is being made toward the identification of sensitive diagnostic tests that do not involve invasive procedures to address a common concern in patients with the nonalcoholic fatty liver disease-whether they have NASH or simple steatosis. We aimed to investigate the independent predictors and develop a non-invasive, easy-to-perform, low-cost set of parameters that may be used in clinical practice to differentiate simple steatosis from NASH. Methods: А cross-sectional study of nonalcoholic fatty liver disease (NAFLD) patients divided into two groups: group I-simple steatosis (SS) and group II-biopsy-proven NASH. Strict inclusion criteria and stepwise analysis allowed the evaluation of a vast number of measured/estimated parameters. Results: One hundred and eleven patients were included-82 with simple steatosis and 29 with biopsy-proven NASH. The probability of NASH was the highest when homeostatic model assessment of insulin resistance (HOMA-IR) was above 2.5, uric acid above 380 µmol/L, ferritin above 100 µg/L and ALT above 45 U/L. An acronym of using first letters was created and named the HUFA index. This combined model resulted in an area under the receiver operator characteristic curve (AUROC) of 0.94, provided sensitivity, specificity, positive predictive value and a negative predictive value for NASH of 70.3%, 95.1%, 83.1% and 90.0%, respectively. Conclusion: We suggest a simple non-invasive predictive index HUFA that encompasses four easily available parameters (HOMA-IR, uric acid, ferritin and ALT) to identify patients with NASH, which may reduce the need for a liver biopsy on a routine basis in patients with NAFLD.",
publisher = "NLM (Medline)",
journal = "Medicina (Kaunas, Lithuania)",
title = "A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests",
volume = "55",
number = "6",
doi = "10.3390/medicina55060243"
}

Ćulafić, M., Vezmar-Kovačević, S., Dopsaj, V., Stulić, M., Vlaisavljević, Ž., Miljković, B.,& Ćulafić, Đ.. (2019). A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests. in Medicina (Kaunas, Lithuania)
NLM (Medline)., 55(6).
https://doi.org/10.3390/medicina55060243

Ćulafić M, Vezmar-Kovačević S, Dopsaj V, Stulić M, Vlaisavljević Ž, Miljković B, Ćulafić Đ. A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests. in Medicina (Kaunas, Lithuania). 2019;55(6).
doi:10.3390/medicina55060243 .

Ćulafić, Milica, Vezmar-Kovačević, Sandra, Dopsaj, Violeta, Stulić, Miloš, Vlaisavljević, Željko, Miljković, Branislava, Ćulafić, Đorđe, "A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests" in Medicina (Kaunas, Lithuania), 55, no. 6 (2019),
https://doi.org/10.3390/medicina55060243 . .

TY  - JOUR
AU  - Radović, Ana
AU  - Milinković, Neda
AU  - Stošović, Milan
AU  - Dopsaj, Violeta
AU  - Ignjatović, Svetlana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3566
AB  - Literature data indicate that increased uric acid (UA) levels are an important risk factor for cardiovascular disease. The aim of this study was to examine the association of  UA with cardiovascular risk factors in patients with hemodialysis using retrospective analysis. In 110 patients,  we  studied  the  values  of  basic  renal  function  parameters,  bone  metabolism  and cardiovascular risk factors in the period from 2010 to 2017. The results indicate a significant increase in urea (P=0.004), creatinine (P=0.028) and inorganic phosphate (P=0.001), as well as significant differences in gender (P=0.013), in risk categories defined on the basis of the UA decision  limit  (cut  off  <350  μmol/L).  After  controlling  the  effects  of  most  parameters, statistically  significant  correlation  coefficients  were  obtained  for  UA  and  urea  (r=0.361; P=0.0013),  creatinine  (r=0.388;  P=0.0005)  and  inorganic  phosphate  (r=0.366;  P=0.0011). Significant regression coefficients were obtained for UA and male gender (β=-0.227, P=0.004), age (β=-0.298, P˂0.001), urea (β=0.271, P=0.005) and inorganic phosphate (β=0.232, P=0.009). The  predictive  value  of  independent  parameters  in  relation  to  UA  was  confirmed  for  male gender  (OR=3.595;  95%  CI:  1.421-9.094;  P=0.007)  and  inorganic  phosphate  (OR=14.842; 2.518-87.472, P=0.003). By Cox regression analysis of proportional hazard ratio, we obtained the most significant combined effect of the body mass index, dialysis and diastolic pressure on UA concentration in relation to the duration of hemodialysis (P <0.0001). The results of this long-term  study  suggest  that  UA  can  not  be  considered  an  independent  cardiovascular  risk factor, but that HD patients need to strategically control the level of MK in order to reduce the resulting complications, morbidity and mortality.
AB  - Literaturni  podaci  ukazuju  da  povećana  koncentracija  mokraćne  kiseline  (MK) predstavlja  značajan  faktor  rizika  za  kardiovaskularne  bolesti.  Cilj  ove  studije  bio  je  da  se retrospektivnom analizom ispita povezanost MK sa faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi. Za 110 bolesnika analizirane su vrednosti osnovnih parametara funkcije bubrega, metabolizma kostiju i faktora rizika za kardiovaskularne bolesti u periodu od 2010. do 2017. godine. Rezultati ukazuju na značajno povećanje koncentracije uree (P=0,004), kreatinina (P=0,028) i neorganskog fosfata (P˂0,001), kao i značajne razlike u zavisnosti od pola (P=0,013) u kategorijama rizika definisanim na osnovu granične koncentracije MK (cut off <350  μmol/L).  Nakon  kontrolisanja  efekta  većine  parametara,  statistički  značajne  vrednosti koeficijenata  korelacije  dobijene  su  za  MK  i  ureu  (r=0,361;  P=0,0013),  kreatinin  (r=0,388; P=0,0005) i neorganski fosfat (r=0,366; P=0,0011). Značajni regresioni koeficijenti su dobijeni za MK i muški pol (β=-0,227, P=0,004), godine starosti (β=-0,298, P˂0,001), ureu (β=0,271, P=0,005) i neorganski fosfat (β=0,232, P=0,009). Prediktivna vrednost nezavisnih parametara u odnosu  na  MK  je  potvrđena  za  muški  pol  (OR=3,595;  95%  CI:  1,421-9,094;  P=0,007)  i neorganski  fosfat  (OR=14,842;  95%  CI:  2,518-87,472,  P=0,003).  Cox-ovom  regresijom proporcionalnih  nepoželjnih  ishoda  dobijen  je  najznačajniji  udruženi  efekat  indeksa  telesne mase  i  adekvatnosti  dijalize  na  koncentraciju  MK  u  odnosu  na  dužinu  trajanja  hemodijalize (P<0,0001). Rezultati ove dugoročne studije ukazuju da se MK ne može smatrati nezavisnim faktorom rizika za kardiovaskularne bolesti, ali da je kod bolesnika na HD, potrebno strateški kontrolisati nivo MK u cilju smanjenja posledičnih komplikacija, morbiditeta i mortaliteta.
PB  - Beograd: Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis
T1  - Povezanost koncentracije mokraćne kiseline sa tradicionalnim i ne-tradicionalnim faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi – retrospektivna analiza
VL  - 69
IS  - 5
SP  - 323
EP  - 337
DO  - 10.5937/arhfarm1905323R
ER  - 

@article{
author = "Radović, Ana and Milinković, Neda and Stošović, Milan and Dopsaj, Violeta and Ignjatović, Svetlana",
year = "2019",
abstract = "Literature data indicate that increased uric acid (UA) levels are an important risk factor for cardiovascular disease. The aim of this study was to examine the association of  UA with cardiovascular risk factors in patients with hemodialysis using retrospective analysis. In 110 patients,  we  studied  the  values  of  basic  renal  function  parameters,  bone  metabolism  and cardiovascular risk factors in the period from 2010 to 2017. The results indicate a significant increase in urea (P=0.004), creatinine (P=0.028) and inorganic phosphate (P=0.001), as well as significant differences in gender (P=0.013), in risk categories defined on the basis of the UA decision  limit  (cut  off  <350  μmol/L).  After  controlling  the  effects  of  most  parameters, statistically  significant  correlation  coefficients  were  obtained  for  UA  and  urea  (r=0.361; P=0.0013),  creatinine  (r=0.388;  P=0.0005)  and  inorganic  phosphate  (r=0.366;  P=0.0011). Significant regression coefficients were obtained for UA and male gender (β=-0.227, P=0.004), age (β=-0.298, P˂0.001), urea (β=0.271, P=0.005) and inorganic phosphate (β=0.232, P=0.009). The  predictive  value  of  independent  parameters  in  relation  to  UA  was  confirmed  for  male gender  (OR=3.595;  95%  CI:  1.421-9.094;  P=0.007)  and  inorganic  phosphate  (OR=14.842; 2.518-87.472, P=0.003). By Cox regression analysis of proportional hazard ratio, we obtained the most significant combined effect of the body mass index, dialysis and diastolic pressure on UA concentration in relation to the duration of hemodialysis (P <0.0001). The results of this long-term  study  suggest  that  UA  can  not  be  considered  an  independent  cardiovascular  risk factor, but that HD patients need to strategically control the level of MK in order to reduce the resulting complications, morbidity and mortality., Literaturni  podaci  ukazuju  da  povećana  koncentracija  mokraćne  kiseline  (MK) predstavlja  značajan  faktor  rizika  za  kardiovaskularne  bolesti.  Cilj  ove  studije  bio  je  da  se retrospektivnom analizom ispita povezanost MK sa faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi. Za 110 bolesnika analizirane su vrednosti osnovnih parametara funkcije bubrega, metabolizma kostiju i faktora rizika za kardiovaskularne bolesti u periodu od 2010. do 2017. godine. Rezultati ukazuju na značajno povećanje koncentracije uree (P=0,004), kreatinina (P=0,028) i neorganskog fosfata (P˂0,001), kao i značajne razlike u zavisnosti od pola (P=0,013) u kategorijama rizika definisanim na osnovu granične koncentracije MK (cut off <350  μmol/L).  Nakon  kontrolisanja  efekta  većine  parametara,  statistički  značajne  vrednosti koeficijenata  korelacije  dobijene  su  za  MK  i  ureu  (r=0,361;  P=0,0013),  kreatinin  (r=0,388; P=0,0005) i neorganski fosfat (r=0,366; P=0,0011). Značajni regresioni koeficijenti su dobijeni za MK i muški pol (β=-0,227, P=0,004), godine starosti (β=-0,298, P˂0,001), ureu (β=0,271, P=0,005) i neorganski fosfat (β=0,232, P=0,009). Prediktivna vrednost nezavisnih parametara u odnosu  na  MK  je  potvrđena  za  muški  pol  (OR=3,595;  95%  CI:  1,421-9,094;  P=0,007)  i neorganski  fosfat  (OR=14,842;  95%  CI:  2,518-87,472,  P=0,003).  Cox-ovom  regresijom proporcionalnih  nepoželjnih  ishoda  dobijen  je  najznačajniji  udruženi  efekat  indeksa  telesne mase  i  adekvatnosti  dijalize  na  koncentraciju  MK  u  odnosu  na  dužinu  trajanja  hemodijalize (P<0,0001). Rezultati ove dugoročne studije ukazuju da se MK ne može smatrati nezavisnim faktorom rizika za kardiovaskularne bolesti, ali da je kod bolesnika na HD, potrebno strateški kontrolisati nivo MK u cilju smanjenja posledičnih komplikacija, morbiditeta i mortaliteta.",
publisher = "Beograd: Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis, Povezanost koncentracije mokraćne kiseline sa tradicionalnim i ne-tradicionalnim faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi – retrospektivna analiza",
volume = "69",
number = "5",
pages = "323-337",
doi = "10.5937/arhfarm1905323R"
}

Radović, A., Milinković, N., Stošović, M., Dopsaj, V.,& Ignjatović, S.. (2019). The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis. in Arhiv za farmaciju
Beograd: Savez farmaceutskih udruženja Srbije., 69(5), 323-337.
https://doi.org/10.5937/arhfarm1905323R

Radović A, Milinković N, Stošović M, Dopsaj V, Ignjatović S. The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis. in Arhiv za farmaciju. 2019;69(5):323-337.
doi:10.5937/arhfarm1905323R .

Radović, Ana, Milinković, Neda, Stošović, Milan, Dopsaj, Violeta, Ignjatović, Svetlana, "The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis" in Arhiv za farmaciju, 69, no. 5 (2019):323-337,
https://doi.org/10.5937/arhfarm1905323R . .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Topić, Aleksandra
AU  - Savković, Miljan
AU  - Milinković, Neda
AU  - Novaković, Ivana
AU  - Cujić, Danica
AU  - Simić-Ogrizović, Sanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3366
AB  - Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.
PB  - Hindawi Ltd, London
T2  - Disease Markers
T1  - Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease
DO  - 10.1155/2019/4864370
ER  - 

@article{
author = "Dopsaj, Violeta and Topić, Aleksandra and Savković, Miljan and Milinković, Neda and Novaković, Ivana and Cujić, Danica and Simić-Ogrizović, Sanja",
year = "2019",
abstract = "Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.",
publisher = "Hindawi Ltd, London",
journal = "Disease Markers",
title = "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease",
doi = "10.1155/2019/4864370"
}

Dopsaj, V., Topić, A., Savković, M., Milinković, N., Novaković, I., Cujić, D.,& Simić-Ogrizović, S.. (2019). Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers
Hindawi Ltd, London..
https://doi.org/10.1155/2019/4864370

Dopsaj V, Topić A, Savković M, Milinković N, Novaković I, Cujić D, Simić-Ogrizović S. Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers. 2019;.
doi:10.1155/2019/4864370 .

Dopsaj, Violeta, Topić, Aleksandra, Savković, Miljan, Milinković, Neda, Novaković, Ivana, Cujić, Danica, Simić-Ogrizović, Sanja, "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease" in Disease Markers (2019),
https://doi.org/10.1155/2019/4864370 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Sarić, Marija
AU  - Stojanović, Ljudmila
AU  - Mirković, Duško
AU  - Dopsaj, Violeta
AU  - Ignjatović, Svetlana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3063
AB  - Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome
VL  - 37
IS  - 12
SP  - 3359
EP  - 3364
DO  - 10.1007/s10067-018-4170-7
ER  - 

@article{
author = "Becarević, Mirjana and Sarić, Marija and Stojanović, Ljudmila and Mirković, Duško and Dopsaj, Violeta and Ignjatović, Svetlana",
year = "2018",
abstract = "Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome",
volume = "37",
number = "12",
pages = "3359-3364",
doi = "10.1007/s10067-018-4170-7"
}

Becarević, M., Sarić, M., Stojanović, L., Mirković, D., Dopsaj, V.,& Ignjatović, S.. (2018). Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 37(12), 3359-3364.
https://doi.org/10.1007/s10067-018-4170-7

Becarević M, Sarić M, Stojanović L, Mirković D, Dopsaj V, Ignjatović S. Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology. 2018;37(12):3359-3364.
doi:10.1007/s10067-018-4170-7 .

Becarević, Mirjana, Sarić, Marija, Stojanović, Ljudmila, Mirković, Duško, Dopsaj, Violeta, Ignjatović, Svetlana, "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome" in Clinical Rheumatology, 37, no. 12 (2018):3359-3364,
https://doi.org/10.1007/s10067-018-4170-7 . .

TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2803
AB  - The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.
PB  - Univ South Bohemia, Ceska Budejovice
T2  - Journal of Applied Biomedicine
T1  - Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function
VL  - 15
IS  - 3
SP  - 204
EP  - 209
DO  - 10.1016/j.jab.2017.02.002
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2017",
abstract = "The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.",
publisher = "Univ South Bohemia, Ceska Budejovice",
journal = "Journal of Applied Biomedicine",
title = "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function",
volume = "15",
number = "3",
pages = "204-209",
doi = "10.1016/j.jab.2017.02.002"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2017). Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine
Univ South Bohemia, Ceska Budejovice., 15(3), 204-209.
https://doi.org/10.1016/j.jab.2017.02.002

Totosković D, Dopsaj V, Martinović J. Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine. 2017;15(3):204-209.
doi:10.1016/j.jab.2017.02.002 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function" in Journal of Applied Biomedicine, 15, no. 3 (2017):204-209,
https://doi.org/10.1016/j.jab.2017.02.002 . .

TY  - CONF
AU  - Jordanova, Elena
AU  - Simić-Ogrizović, Sanja
AU  - Samardžić, Vladimir
AU  - Dopsaj, Violeta
AU  - Bokan, Ljiljana
AU  - Peruničič-Peković, Gordana
AU  - Tica-Jeftić, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2931
PB  - Oxford Univ Press, Oxford
C3  - Nephrology Dialysis Transplantation
T1  - Is there any importance to determine of uromodulin serum concentration in patients with long term diabetes mellitus type 2
VL  - 32
IS  - Supplement 3
SP  - iii545
EP  - iii545
DO  - 10.1093/ndt/gfx168.MP325
ER  - 

@conference{
author = "Jordanova, Elena and Simić-Ogrizović, Sanja and Samardžić, Vladimir and Dopsaj, Violeta and Bokan, Ljiljana and Peruničič-Peković, Gordana and Tica-Jeftić, Jelena",
year = "2017",
publisher = "Oxford Univ Press, Oxford",
journal = "Nephrology Dialysis Transplantation",
title = "Is there any importance to determine of uromodulin serum concentration in patients with long term diabetes mellitus type 2",
volume = "32",
number = "Supplement 3",
pages = "iii545-iii545",
doi = "10.1093/ndt/gfx168.MP325"
}

Jordanova, E., Simić-Ogrizović, S., Samardžić, V., Dopsaj, V., Bokan, L., Peruničič-Peković, G.,& Tica-Jeftić, J.. (2017). Is there any importance to determine of uromodulin serum concentration in patients with long term diabetes mellitus type 2. in Nephrology Dialysis Transplantation
Oxford Univ Press, Oxford., 32(Supplement 3), iii545-iii545.
https://doi.org/10.1093/ndt/gfx168.MP325

Jordanova E, Simić-Ogrizović S, Samardžić V, Dopsaj V, Bokan L, Peruničič-Peković G, Tica-Jeftić J. Is there any importance to determine of uromodulin serum concentration in patients with long term diabetes mellitus type 2. in Nephrology Dialysis Transplantation. 2017;32(Supplement 3):iii545-iii545.
doi:10.1093/ndt/gfx168.MP325 .

Jordanova, Elena, Simić-Ogrizović, Sanja, Samardžić, Vladimir, Dopsaj, Violeta, Bokan, Ljiljana, Peruničič-Peković, Gordana, Tica-Jeftić, Jelena, "Is there any importance to determine of uromodulin serum concentration in patients with long term diabetes mellitus type 2" in Nephrology Dialysis Transplantation, 32, no. Supplement 3 (2017):iii545-iii545,
https://doi.org/10.1093/ndt/gfx168.MP325 . .

TY  - CONF
AU  - Stojanović, Ljudmila
AU  - Stanisavljević, Nataša
AU  - Marisavljević, D.
AU  - Dopsaj, Violeta
AU  - Todić, B.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2648
PB  - BMJ PUBLISHING GROUP, LONDON
C3  - Annals of the Rheumatic Diseases
T1  - The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome
VL  - 75
IS  - Supplement 2
SP  - 284
EP  - 284
DO  - 10.1136/annrheumdis-2016-eular.1782
ER  - 

@conference{
author = "Stojanović, Ljudmila and Stanisavljević, Nataša and Marisavljević, D. and Dopsaj, Violeta and Todić, B.",
year = "2016",
publisher = "BMJ PUBLISHING GROUP, LONDON",
journal = "Annals of the Rheumatic Diseases",
title = "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome",
volume = "75",
number = "Supplement 2",
pages = "284-284",
doi = "10.1136/annrheumdis-2016-eular.1782"
}

Stojanović, L., Stanisavljević, N., Marisavljević, D., Dopsaj, V.,& Todić, B.. (2016). The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases
BMJ PUBLISHING GROUP, LONDON., 75(Supplement 2), 284-284.
https://doi.org/10.1136/annrheumdis-2016-eular.1782

Stojanović L, Stanisavljević N, Marisavljević D, Dopsaj V, Todić B. The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases. 2016;75(Supplement 2):284-284.
doi:10.1136/annrheumdis-2016-eular.1782 .

Stojanović, Ljudmila, Stanisavljević, Nataša, Marisavljević, D., Dopsaj, Violeta, Todić, B., "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome" in Annals of the Rheumatic Diseases, 75, no. Supplement 2 (2016):284-284,
https://doi.org/10.1136/annrheumdis-2016-eular.1782 . .

TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanović, Ljudmila
AU  - Marisavljević, D.
AU  - Đoković, A.
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Martinović, Jelena
AU  - Memon, Lidija
AU  - Radovanović, Slavica
AU  - Todić, B.
AU  - Lisulov, D.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2710
AB  - The aim of this study was to evaluate oxidative stress markers and it relations to endothelial damage as risk factor for thrombosis in patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome (APS) in correlation to traditional risk factors. Flow-mediated (FMD) and nitroglycerine (NMD)-induced dilation of the brachial artery were studied in 140 APS patients (90 PAPS, 50 SAPS) and 40 controls matched by age, sex, and conventional risk factors for atherosclerosis. Markers of oxidative stress, lipid hydroperoxydes (LOOH), advanced oxidation protein products (AOPP), total sulfhydryl groups (tSHG), and paraoxonase 1 activity (PON1) were determined by spectrophotometric method. Oxidative stress dominates in APS patients. LOOH and AOPP correlate to lipid fractions (p  lt  0.05), unlike PON1, tSHG that correlated to antiphospholipid antibody positivity (p  lt  0.05). FMD was lower in APS patients comparing to controls (p  lt  0.001). Cholesterol is independent variable for FMD impairment in control group (p = 0.011); LOOH in PAPS (p = 0.004); LOOH, aCL, and triglycerides in SAPS patients (p = 0.009, p = 0.049, and p = 0.012, respectively). Combined predictive of aCL and LOOH is better for FMD impairment than LOOH alone in both PAPS and SAPS patients (AUC 0.727, p = 0.001, 95 % CI 0.616-0.837 and AUC 0.824, pE,0.001, 95 % CI 0.690-0.957, respectively). Lipid peroxidation is independent predictor for endothelial dysfunction in APS patients. We demonstrated synergistic effect of aCL and LOOH as risk for endothelial impairment in both PAPS and SAPS patients.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients
VL  - 35
IS  - 10
SP  - 2485
EP  - 2493
DO  - 10.1007/s10067-016-3369-8
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanović, Ljudmila and Marisavljević, D. and Đoković, A. and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Martinović, Jelena and Memon, Lidija and Radovanović, Slavica and Todić, B. and Lisulov, D.",
year = "2016",
abstract = "The aim of this study was to evaluate oxidative stress markers and it relations to endothelial damage as risk factor for thrombosis in patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome (APS) in correlation to traditional risk factors. Flow-mediated (FMD) and nitroglycerine (NMD)-induced dilation of the brachial artery were studied in 140 APS patients (90 PAPS, 50 SAPS) and 40 controls matched by age, sex, and conventional risk factors for atherosclerosis. Markers of oxidative stress, lipid hydroperoxydes (LOOH), advanced oxidation protein products (AOPP), total sulfhydryl groups (tSHG), and paraoxonase 1 activity (PON1) were determined by spectrophotometric method. Oxidative stress dominates in APS patients. LOOH and AOPP correlate to lipid fractions (p  lt  0.05), unlike PON1, tSHG that correlated to antiphospholipid antibody positivity (p  lt  0.05). FMD was lower in APS patients comparing to controls (p  lt  0.001). Cholesterol is independent variable for FMD impairment in control group (p = 0.011); LOOH in PAPS (p = 0.004); LOOH, aCL, and triglycerides in SAPS patients (p = 0.009, p = 0.049, and p = 0.012, respectively). Combined predictive of aCL and LOOH is better for FMD impairment than LOOH alone in both PAPS and SAPS patients (AUC 0.727, p = 0.001, 95 % CI 0.616-0.837 and AUC 0.824, pE,0.001, 95 % CI 0.690-0.957, respectively). Lipid peroxidation is independent predictor for endothelial dysfunction in APS patients. We demonstrated synergistic effect of aCL and LOOH as risk for endothelial impairment in both PAPS and SAPS patients.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients",
volume = "35",
number = "10",
pages = "2485-2493",
doi = "10.1007/s10067-016-3369-8"
}

Stanisavljević, N., Stojanović, L., Marisavljević, D., Đoković, A., Dopsaj, V., Kotur-Stevuljević, J., Martinović, J., Memon, L., Radovanović, S., Todić, B.,& Lisulov, D.. (2016). Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients. in Clinical Rheumatology
Springer London Ltd, London., 35(10), 2485-2493.
https://doi.org/10.1007/s10067-016-3369-8

Stanisavljević N, Stojanović L, Marisavljević D, Đoković A, Dopsaj V, Kotur-Stevuljević J, Martinović J, Memon L, Radovanović S, Todić B, Lisulov D. Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients. in Clinical Rheumatology. 2016;35(10):2485-2493.
doi:10.1007/s10067-016-3369-8 .

Stanisavljević, Nataša, Stojanović, Ljudmila, Marisavljević, D., Đoković, A., Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Martinović, Jelena, Memon, Lidija, Radovanović, Slavica, Todić, B., Lisulov, D., "Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients" in Clinical Rheumatology, 35, no. 10 (2016):2485-2493,
https://doi.org/10.1007/s10067-016-3369-8 . .

TY  - CONF
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2697
PB  - Wiley-Blackwell, Hoboken
C3  - International Journal of Laboratory Hematology
T1  - Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment
VL  - 38
SP  - 107
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2697
ER  - 

@conference{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment",
volume = "38",
pages = "107-108",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2697"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 38, 107-108.
https://hdl.handle.net/21.15107/rcub_farfar_2697

Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment. in International Journal of Laboratory Hematology. 2016;38:107-108.
https://hdl.handle.net/21.15107/rcub_farfar_2697 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment" in International Journal of Laboratory Hematology, 38 (2016):107-108,
https://hdl.handle.net/21.15107/rcub_farfar_2697 .

TY  - JOUR
AU  - Alempijević, Tamara
AU  - Zec, Simon
AU  - Nikolić, Vladimir
AU  - Veljković, Aleksandar
AU  - Milivojević, Vladimir
AU  - Dopsaj, Violeta
AU  - Stanković, Sanja
AU  - Milosavljević, Tomica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2670
AB  - Background & Aims: Acute-on-chronic liver failure (ACLF) is characterized by a rapid progression to multiple organ failure and is associated with a very high mortality rate of 50-90%. Novel therapies are being investigated such as Erythropoietin (EPO). The aim of this prospective cohort study was to analyse the value of EPO in predicting prognosis and determine which patients may benefit most from EPO therapy. Methods: According to the EASL-CLIF criteria, 104 consecutive patients were diagnosed with ACLF, and separated into two groups based on the type of insult: bleeding (Group A=31) or non-bleeding (Group B=73). In addition to a complete biochemical work-up and calculation of relevant prognostic scores, levels of EPO were measured on admission and correlated to the type of insult and final outcome. Results: Fifteen patients from Group A (mean age 60.32 +/- 9.29 years) had a lethal outcome and higher values of EPO on admission (319.26 +/- 326.58 mIU/ml) (p lt 0.005), compared to the 37 patients from Group B (mean age 59.9 +/- 10.19 years) with EPO levels at admission of 29.88 +/- 34.6 mIU/mL. In Group B, a cut-off EPO value of 30.65 mIU/mL had a sensitivity of 87.5% and a specificity 57.4% in predicting lethal outcome with an AUROC of 0.823. In Group A, a cut-off value of 229.95 mlU/mL had a sensitivity and specificity of 53.3% and 92.7%, respectively. The AUROC for this cut-off was 0.847. Conclusions: Erythropoietin is superior to the standard prognostic scores in predicting 28-day mortality. Lower levels of EPO were detected in patients without bleeding as an insult indicating a possible therapeutic benefit in these patients.
PB  - Medical Univ Press, Cluj-Napoca
T2  - Journal of Gastrointestinal and Liver Diseases
T1  - Erythropoietin in Predicting Prognosis in Patients with Acute-on-Chronic Liver Failure
VL  - 25
IS  - 4
SP  - 473
EP  - 479
DO  - 10.15403/jgld.2014.1121.254.jev
ER  - 

@article{
author = "Alempijević, Tamara and Zec, Simon and Nikolić, Vladimir and Veljković, Aleksandar and Milivojević, Vladimir and Dopsaj, Violeta and Stanković, Sanja and Milosavljević, Tomica",
year = "2016",
abstract = "Background & Aims: Acute-on-chronic liver failure (ACLF) is characterized by a rapid progression to multiple organ failure and is associated with a very high mortality rate of 50-90%. Novel therapies are being investigated such as Erythropoietin (EPO). The aim of this prospective cohort study was to analyse the value of EPO in predicting prognosis and determine which patients may benefit most from EPO therapy. Methods: According to the EASL-CLIF criteria, 104 consecutive patients were diagnosed with ACLF, and separated into two groups based on the type of insult: bleeding (Group A=31) or non-bleeding (Group B=73). In addition to a complete biochemical work-up and calculation of relevant prognostic scores, levels of EPO were measured on admission and correlated to the type of insult and final outcome. Results: Fifteen patients from Group A (mean age 60.32 +/- 9.29 years) had a lethal outcome and higher values of EPO on admission (319.26 +/- 326.58 mIU/ml) (p lt 0.005), compared to the 37 patients from Group B (mean age 59.9 +/- 10.19 years) with EPO levels at admission of 29.88 +/- 34.6 mIU/mL. In Group B, a cut-off EPO value of 30.65 mIU/mL had a sensitivity of 87.5% and a specificity 57.4% in predicting lethal outcome with an AUROC of 0.823. In Group A, a cut-off value of 229.95 mlU/mL had a sensitivity and specificity of 53.3% and 92.7%, respectively. The AUROC for this cut-off was 0.847. Conclusions: Erythropoietin is superior to the standard prognostic scores in predicting 28-day mortality. Lower levels of EPO were detected in patients without bleeding as an insult indicating a possible therapeutic benefit in these patients.",
publisher = "Medical Univ Press, Cluj-Napoca",
journal = "Journal of Gastrointestinal and Liver Diseases",
title = "Erythropoietin in Predicting Prognosis in Patients with Acute-on-Chronic Liver Failure",
volume = "25",
number = "4",
pages = "473-479",
doi = "10.15403/jgld.2014.1121.254.jev"
}

Alempijević, T., Zec, S., Nikolić, V., Veljković, A., Milivojević, V., Dopsaj, V., Stanković, S.,& Milosavljević, T.. (2016). Erythropoietin in Predicting Prognosis in Patients with Acute-on-Chronic Liver Failure. in Journal of Gastrointestinal and Liver Diseases
Medical Univ Press, Cluj-Napoca., 25(4), 473-479.
https://doi.org/10.15403/jgld.2014.1121.254.jev

Alempijević T, Zec S, Nikolić V, Veljković A, Milivojević V, Dopsaj V, Stanković S, Milosavljević T. Erythropoietin in Predicting Prognosis in Patients with Acute-on-Chronic Liver Failure. in Journal of Gastrointestinal and Liver Diseases. 2016;25(4):473-479.
doi:10.15403/jgld.2014.1121.254.jev .

Alempijević, Tamara, Zec, Simon, Nikolić, Vladimir, Veljković, Aleksandar, Milivojević, Vladimir, Dopsaj, Violeta, Stanković, Sanja, Milosavljević, Tomica, "Erythropoietin in Predicting Prognosis in Patients with Acute-on-Chronic Liver Failure" in Journal of Gastrointestinal and Liver Diseases, 25, no. 4 (2016):473-479,
https://doi.org/10.15403/jgld.2014.1121.254.jev . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Stojanović, Ljudmila
AU  - Ignjatović, Svetlana
AU  - Dopsaj, Violeta
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2590
AB  - We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome
VL  - 35
IS  - 5
SP  - 1361
EP  - 1365
DO  - 10.1007/s10067-016-3230-0
ER  - 

@article{
author = "Becarević, Mirjana and Stojanović, Ljudmila and Ignjatović, Svetlana and Dopsaj, Violeta",
year = "2016",
abstract = "We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome",
volume = "35",
number = "5",
pages = "1361-1365",
doi = "10.1007/s10067-016-3230-0"
}

Becarević, M., Stojanović, L., Ignjatović, S.,& Dopsaj, V.. (2016). The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 35(5), 1361-1365.
https://doi.org/10.1007/s10067-016-3230-0

Becarević M, Stojanović L, Ignjatović S, Dopsaj V. The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology. 2016;35(5):1361-1365.
doi:10.1007/s10067-016-3230-0 .

Becarević, Mirjana, Stojanović, Ljudmila, Ignjatović, Svetlana, Dopsaj, Violeta, "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome" in Clinical Rheumatology, 35, no. 5 (2016):1361-1365,
https://doi.org/10.1007/s10067-016-3230-0 . .

TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2589
AB  - IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis
VL  - 38
IS  - 3
SP  - 265
EP  - 272
DO  - 10.1111/ijlh.12479
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2016",
abstract = "IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis",
volume = "38",
number = "3",
pages = "265-272",
doi = "10.1111/ijlh.12479"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 38(3), 265-272.
https://doi.org/10.1111/ijlh.12479

Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology. 2016;38(3):265-272.
doi:10.1111/ijlh.12479 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis" in International Journal of Laboratory Hematology, 38, no. 3 (2016):265-272,
https://doi.org/10.1111/ijlh.12479 . .