@conference{
author = "Radović, Jelena and Grozdanić, Nađa and Stanojković, Tatjana and Suručić, Relja and Kundaković-Vasović, Tatjana",
year = "2018",
abstract = "Tanini, polifenolni biljni metaboliti, značajno smanjuju postprandijalnu
hiperglikemiju inhibicijom α‐glukozidaze, i stoga mogu biti efikasna strategija u
kontroli dijabetesa tipa 2. Takođe, dokazano je i da nespecifično inhibiraju aktivnost
angiotenzin‐konvertujućeg enzima (ACE). Kako su tanini identifikovani samo u vrsti
Alchemilla vulgaris L., cilj ovog istraživanja je da se odredi sadržaj tanina u do sada
neistraženoj vrsti A. viridiflora Rothm. (Rosaceae), kao i inhibitorni uticaj na aktivnost
ACE i α‐glukozidaze.
Ukupni sadržaj tanina u metanolnom ekstraktu A. viridiflora određen je prema
propisu Ph. Eur. 9.0. Suvi metanolni ekstrakt, enzimski rastvor (400 mU/ml α‐
glukozidaze u 0,1 M fosfatnom puferu) i supstrat, p‐nitrofenil α‐D‐glukopiranozid
korišćeni su za kolorimetrijski test inhibitorne aktivnosti α‐glukozidaze. Kao pozitivna
kontrola korišćena je akarboza. ACE inhibitorna aktivnost metanolnog ekstrakta
ispitana je korišćenjem komercijalnog testa ACE Kit‐ WST (Dojindo Inc., Japan) prema
uputstvu proizvođača. Procenat inhibicije enzima je izračunata IC50 vrednost, tj.
procenjena koncentracija ekstrakta koja je izazvala 50% inhibicije aktivnosti enzima,
koristeći linearnu regresionu analizu.
IC50 vrednost metanolnog ekstrakta A. viridiflora, očitana sa dozno‐zavisne krive
iznosi 2,6±0,5 μg/ml, i ekstrakt pokazuje bolju anti‐α‐glukozidaznu aktivnost od
standarda akarboze (IC50=74,2±3,3 μg/ml). Takođe, ispitivani ekstrakt pokazuje doznozavisnu
inhibiciju ACE pri IC50 2 μg/ml. Dobijeni rezultati su u korelaciji sa visokim
sadržajem tanina u metanolnom ekstraktu A. viridiflora (3,74 %).
Pokazane inhibicije angiotenzin‐konvertujućeg enzima i α‐glukozidaze čine
metanolni ekstrakt vrste A. viridiflora pogodnim za dalje istraživanje u cilju
pronalaženja novih prirodnih proizvoda značajnih za terapiju kardiovaskularnih bolesti
i dijabetesa., Tannins, polyphenolic plant metabolites, significantly reduce postprandial
hyperglycemia by inhibiting α‐glucosidase, and therefore can be an effective strategy
for controlling type 2 diabetes. It has also been proven that they are non‐specific
inhibitors of the activity of angiotensin‐converting enzyme (ACE). As tannins were
identified only in the species Alchemilla vulgaris L., the aim of this study is to determine
the content of tannins in the unexplored A. viridiflora Rothm. (Rosaceae), as well as the
inhibitory effect on the activity of angiotensin‐converting enzyme and α‐glucosidase.
The content of tannins in methanol extract of A. viridiflora was determined
according to the Ph. Eur. 9.0. Dry methanol extract, enzyme solution (400 mU/ml of α‐
glucosidase in 0.1 M phosphate buffer) and substrate, p‐nitrophenyl α‐Dglucopyranoside
were used for colorimetric α‐glucosidase inhibitory activity test.
Acarbose was used as a positive control. The ACE inhibitory activity of the methanol
extract was tested using the commercial ACE Kit‐WST (Dojindo Inc., Japan) according to
the manufacturer's instructions. The percentage of enzyme inhibition is the calculated
IC50 value, i.e. estimated concentration of the extract that caused 50% inhibition of
enzyme activity using linear regression analysis.
The IC50 of A. viridiflora methanol extract, read from the dose‐dependent curve,
was 2.6±0.5 μg/mL, and this extract demonstrated better anti‐α‐glucosidase activity
than standard acarbose (IC50=74.2±3.3 μg/mL). In addition, the examined extract
shows a dose‐dependent inhibition of ACE with IC50 2 μg/mL. Obtained results were in
correlation with high level of tannins in methanol extract of A. viridiflora (3.74%).
The proven inhibitions of ACE and α-glucosidase make the methanol extract
of A. viridiflora suitable for further scientific research in order to find a new natural
product for the treatment of cardiovascular diseases and diabetes.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "ACE i α‐glukozidazna inhibitorna aktivnost metanolnog ekstrakta Alchemilla viridiflora Rothm. (Rosaceae), ACE and α‐glucosidase inhibitory activity of methanol extract of Alchemilla viridiflora Rothm. (Rosaceae)",
volume = "68",
number = "2",
pages = "342-343",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5277"
}
