TY  - JOUR
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Savić, Miroslav
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Jančić, Ivan
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4910
AB  - Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords
AB  - Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.
PB  - De Gruyter Poland
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Is alumina suitable for solid phase extraction of catecholamines from brain tissue?
T1  - Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?
VL  - 74
IS  - 2
SP  - 120
EP  - 126
DO  - 10.2478/aiht-2023-74-3706
ER  - 

@article{
author = "Mirković, Duško and Beletić, Anđelo and Savić, Miroslav and Milinković, Neda and Sarić-Matutinović, Marija and Jančić, Ivan",
year = "2023",
abstract = "Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords, Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.",
publisher = "De Gruyter Poland",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?, Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?",
volume = "74",
number = "2",
pages = "120-126",
doi = "10.2478/aiht-2023-74-3706"
}

Mirković, D., Beletić, A., Savić, M., Milinković, N., Sarić-Matutinović, M.,& Jančić, I.. (2023). Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju
De Gruyter Poland., 74(2), 120-126.
https://doi.org/10.2478/aiht-2023-74-3706

Mirković D, Beletić A, Savić M, Milinković N, Sarić-Matutinović M, Jančić I. Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju. 2023;74(2):120-126.
doi:10.2478/aiht-2023-74-3706 .

Mirković, Duško, Beletić, Anđelo, Savić, Miroslav, Milinković, Neda, Sarić-Matutinović, Marija, Jančić, Ivan, "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?" in Arhiv za higijenu rada i toksikologiju, 74, no. 2 (2023):120-126,
https://doi.org/10.2478/aiht-2023-74-3706 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3588
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
DO  - 10.1016/j.vetpar.2020.109140
ER  - 

@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević Filipović, Milica",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
doi = "10.1016/j.vetpar.2020.109140"
}

Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević Filipović, M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology
Elsevier., 282.
https://doi.org/10.1016/j.vetpar.2020.109140

Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević Filipović M. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282.
doi:10.1016/j.vetpar.2020.109140 .

Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević Filipović, Milica, "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020),
https://doi.org/10.1016/j.vetpar.2020.109140 . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Đorđević, Valentina
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2299
AB  - Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease
VL  - 34
IS  - 4
SP  - 467
EP  - 472
DO  - 10.2478/jomb-2014-0046
ER  - 

@article{
author = "Beletić, Anđelo and Mirković, Duško and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Đorđević, Valentina and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2015",
abstract = "Background: An increased homocysteine (Hcy) concentration may represent a metabolic marker of folate and vitamin B-12 deficiency, both significant public health problems. For different reasons, patients with chronic obstructive pulmonary disease (COPD) are prone to these deficiencies. The study evaluates the reliability of Hcy concentration in predicting folate or vitamin B-12 deficiency in these patients. Methods: A group of 50 COPD patients (28 males/22 females, age ((X) over bar +/- SD=49.0 +/- 14.5) years was enrolled. A chemiluminescent microparticle immunoassay was applied for homocysteine, folate and vitamin B-12 concentration. Kolmogorov-Smirnov, Mann-Whitney U and chi(2) tests, Spearman's correlation and ROC analysis were included in the statistical analysis, with the level of significance set at 0.05. Results: Average (SD) concentrations of folate and vitamin B-12 were 4.13 (2.16) mu g/L and 463.6 (271.0) ng/L, whereas only vitamin B-12 correlated with the Hcy level (P=-0.310 (R=0.029)). Gender related differences were not significant and only a borderline significant correlation between age and folate was confirmed (R=0.279 (P=0.047)). The incidence of folate and vitamin B-12 deficiency differed significantly (P=0.000 and P lt 0.000 for folate and vitamin B12 respectively), depending on the cutoff used for classification (4.4, 6.6 and 8.0 mu g/L folate; 203 and 473 ng/L - vitamin B-12). ROC analyses failed to show any significance of hyperhomocysteinemia as a predictor of folate or vitamin B-12 deficiency. Conclusion: Reliability of the Hcy concentration as a biomarker of folate or vitamin B-12 depletion in COPD patients is not satisfactory, so their deficiency cannot be predicted by the occurrence of HHcy.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease",
volume = "34",
number = "4",
pages = "467-472",
doi = "10.2478/jomb-2014-0046"
}

Beletić, A., Mirković, D., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Đorđević, V., Ignjatović, S.,& Majkić-Singh, N.. (2015). Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 34(4), 467-472.
https://doi.org/10.2478/jomb-2014-0046

Beletić A, Mirković D, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Đorđević V, Ignjatović S, Majkić-Singh N. Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease. in Journal of Medical Biochemistry. 2015;34(4):467-472.
doi:10.2478/jomb-2014-0046 .

Beletić, Anđelo, Mirković, Duško, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Đorđević, Valentina, Ignjatović, Svetlana, Majkić-Singh, Nada, "Questionable Reliability of Homocysteine As the Metabolic Marker for Folate and Vitamin B12 Deficiency in Patients with Chronic Obstructive Pulmonary Disease" in Journal of Medical Biochemistry, 34, no. 4 (2015):467-472,
https://doi.org/10.2478/jomb-2014-0046 . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Dudvarski-Ilić, Aleksandra
AU  - Milenković, Branislava
AU  - Nagorni-Obradović, Ljudmila
AU  - Ljujić, Mila
AU  - Đorđević, Valentina
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2204
AB  - Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.
PB  - Croatian Soc Medical Biochemists, Zagreb
T2  - Biochemia Medica
T1  - Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?
VL  - 24
IS  - 2
SP  - 293
EP  - 298
DO  - 10.11613/BM.2014.032
ER  - 

@article{
author = "Beletić, Anđelo and Dudvarski-Ilić, Aleksandra and Milenković, Branislava and Nagorni-Obradović, Ljudmila and Ljujić, Mila and Đorđević, Valentina and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2014",
abstract = "Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness of an integrative laboratory algorithm for AATD detection in patients diagnosed with COPD by the age of 45 years, in comparison with the screening approach based on AAT concentration measurement alone. Subjects and methods: 50 unrelated patients (28 males / 22 females, age 52 (24-75 years) diagnosed with COPD before the age of 45 years were enrolled. Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S allele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests. Results: AATD associated genotypes were detected in 7 patients (5 ZZ, 1 ZM(malton), 1 ZQ0(amersfoort)), 10 were heterozygous carriers (8 MZ and 2 MS genotypes) and 33 were without AATD (MM genotype). Carriers and patients without AATD had comparable AAT concentrations (P = 0.125). In majority of participants (48) first line tests were sufficient to analyze AATD presence. In two remaining cases reflex tests identified rare alleles, M-malton and Q0(amersfoort), the later one being reported for the first time in Serbian population. Detection rate did not differ between algorithm and screening both for AATD (P = 0.500) and carriers (P = 0.063). Conclusion: There is a high prevalence of AATD affected subjects and carriers in a group of patients with premature COPD. The use of integrative laboratory algorithm does not improve the effectiveness of AATD detection in comparison with the screening based on AAT concentration alone.",
publisher = "Croatian Soc Medical Biochemists, Zagreb",
journal = "Biochemia Medica",
title = "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?",
volume = "24",
number = "2",
pages = "293-298",
doi = "10.11613/BM.2014.032"
}

Beletić, A., Dudvarski-Ilić, A., Milenković, B., Nagorni-Obradović, L., Ljujić, M., Đorđević, V., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2014). Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica
Croatian Soc Medical Biochemists, Zagreb., 24(2), 293-298.
https://doi.org/10.11613/BM.2014.032

Beletić A, Dudvarski-Ilić A, Milenković B, Nagorni-Obradović L, Ljujić M, Đorđević V, Mirković D, Radojković D, Majkić-Singh N. Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?. in Biochemia Medica. 2014;24(2):293-298.
doi:10.11613/BM.2014.032 .

Beletić, Anđelo, Dudvarski-Ilić, Aleksandra, Milenković, Branislava, Nagorni-Obradović, Ljudmila, Ljujić, Mila, Đorđević, Valentina, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Is an integrative laboratory algorithm more effective in detecting alpha-1-antitrypsin deficiency in patients with premature chronic obstructive pulmonary disease than AAT concentration based screening approach?" in Biochemia Medica, 24, no. 2 (2014):293-298,
https://doi.org/10.11613/BM.2014.032 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1656
AB  - Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L).
AB  - Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience
T1  - Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo
VL  - 31
IS  - 4
SP  - 347
EP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1656
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Beletić, Anđelo and Mirković, Duško and Majkić-Singh, Nada",
year = "2012",
abstract = "Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L)., Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience, Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo",
volume = "31",
number = "4",
pages = "347-357",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1656"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Beletić, A., Mirković, D.,& Majkić-Singh, N.. (2012). Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656

Jovičić S, Ignjatović S, Kangrga R, Beletić A, Mirković D, Majkić-Singh N. Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry. 2012;31(4):347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Beletić, Anđelo, Mirković, Duško, Majkić-Singh, Nada, "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience" in Journal of Medical Biochemistry, 31, no. 4 (2012):347-357,
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

TY  - CONF
AU  - Beletić, Anđelo
AU  - Đorđević, Valentina
AU  - Canić, I.
AU  - Kocica, T.
AU  - Kuzmanović, I.
AU  - Golubović, Milka
AU  - Mirković, Duško
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1531
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1531
ER  - 

@conference{
author = "Beletić, Anđelo and Đorđević, Valentina and Canić, I. and Kocica, T. and Kuzmanović, I. and Golubović, Milka and Mirković, Duško and Radojković, Dragica and Majkić-Singh, Nada",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1531"
}

Beletić, A., Đorđević, V., Canić, I., Kocica, T., Kuzmanović, I., Golubović, M., Mirković, D., Radojković, D.,& Majkić-Singh, N.. (2011). Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1531

Beletić A, Đorđević V, Canić I, Kocica T, Kuzmanović I, Golubović M, Mirković D, Radojković D, Majkić-Singh N. Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

Beletić, Anđelo, Đorđević, Valentina, Canić, I., Kocica, T., Kuzmanović, I., Golubović, Milka, Mirković, Duško, Radojković, Dragica, Majkić-Singh, Nada, "Experiences in simultaneous detection of factor v leiden, factor ii g20210a, mthfr c677t and mthfr a1298c mutations in patients with thrombophilia" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1531 .

TY  - CONF
AU  - Mirković, Duško
AU  - Ćulafić, Đorđe
AU  - Rudić, J. S.
AU  - Beletić, Anđelo
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1530
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Importance of serotonin determination in patients with esophageal and gastric fundus varices
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1530
ER  - 

@conference{
author = "Mirković, Duško and Ćulafić, Đorđe and Rudić, J. S. and Beletić, Anđelo",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Importance of serotonin determination in patients with esophageal and gastric fundus varices",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1530"
}

Mirković, D., Ćulafić, Đ., Rudić, J. S.,& Beletić, A.. (2011). Importance of serotonin determination in patients with esophageal and gastric fundus varices. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1530

Mirković D, Ćulafić Đ, Rudić JS, Beletić A. Importance of serotonin determination in patients with esophageal and gastric fundus varices. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1530 .

Mirković, Duško, Ćulafić, Đorđe, Rudić, J. S., Beletić, Anđelo, "Importance of serotonin determination in patients with esophageal and gastric fundus varices" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1530 .

TY  - JOUR
AU  - Radovanović, N.
AU  - Antonijević, N.
AU  - Beletić, Anđelo
AU  - Perunicić, J.
AU  - Kocica, Mladen J.
AU  - Mirković, Duško
AU  - Lacković, Vesna
AU  - Lacković, Milena
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1326
AB  - Investigation of hyperhomocysteinemia (HHcy) as an important risk factor for pulmonary thromboembolism (PTE), which represents a most dangerous consequence of a unique phenomenon of venous thromboembolism which still suffers from sometimes conflicting or inadequately clarified results. The role of homocysteine in the clinical manifestation of this life-threatening disease and its treatment (in which any further information may be decisive) requires detailed examination. The purpose of this study is to determine the differences in HHcy incidence and homocysteinemia levels between patients with PTE and healthy persons. The study enrolled 70 patients with PTE and 50 healthy persons. Homocysteine was measured using the HPLC method with fluorescent detection and HHcy was defined as homocysteinemia above 12 mu mol/L. Statistical analyses included chi-square and Mann Whitney U tests. The median homocysteinemia value was significantly higher (p=0.017) in the patients (12.10 mu mol/L) than in the controls (10.35 mu mol/L). The comparison of HHcy incidence between the patients (51.5%) and controls (30%) revealed a significant difference (p=0.021). In patients, homocysteinemia was significantly higher (p=0.002) in men (14.05 mu mol/L) than in women (10.01 mu mol/L) HHcy was present in 67.6% of men with PTE, which was significantly higher (p=0.006) than the incidence in women with PTE (33.3%). Healthy males had significantly higher (p=0.001) homocysteinemia (12.54 mu mol/L) than healthy females (9.4 mu mol/L). A significant difference (p=0.031) was observed between the incidences of HHcy in healthy males (44.0%) and healthy females (16.0%). We conclude that the incidence of hyperhomocysteinemia and homocysteinemia are significantly higher in all the patients compared with de healthy persons, as well as in both healthy males and males with PTE compared with healthy females and female patients. This indicates that HHcy findings in PE are likely to have a clinical importance.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Hyperhomocysteinemia in patients with pulmonary embolism
VL  - 62
IS  - 4
SP  - 907
EP  - 914
DO  - 10.2298/ABS1004907R
ER  - 

@article{
author = "Radovanović, N. and Antonijević, N. and Beletić, Anđelo and Perunicić, J. and Kocica, Mladen J. and Mirković, Duško and Lacković, Vesna and Lacković, Milena",
year = "2010",
abstract = "Investigation of hyperhomocysteinemia (HHcy) as an important risk factor for pulmonary thromboembolism (PTE), which represents a most dangerous consequence of a unique phenomenon of venous thromboembolism which still suffers from sometimes conflicting or inadequately clarified results. The role of homocysteine in the clinical manifestation of this life-threatening disease and its treatment (in which any further information may be decisive) requires detailed examination. The purpose of this study is to determine the differences in HHcy incidence and homocysteinemia levels between patients with PTE and healthy persons. The study enrolled 70 patients with PTE and 50 healthy persons. Homocysteine was measured using the HPLC method with fluorescent detection and HHcy was defined as homocysteinemia above 12 mu mol/L. Statistical analyses included chi-square and Mann Whitney U tests. The median homocysteinemia value was significantly higher (p=0.017) in the patients (12.10 mu mol/L) than in the controls (10.35 mu mol/L). The comparison of HHcy incidence between the patients (51.5%) and controls (30%) revealed a significant difference (p=0.021). In patients, homocysteinemia was significantly higher (p=0.002) in men (14.05 mu mol/L) than in women (10.01 mu mol/L) HHcy was present in 67.6% of men with PTE, which was significantly higher (p=0.006) than the incidence in women with PTE (33.3%). Healthy males had significantly higher (p=0.001) homocysteinemia (12.54 mu mol/L) than healthy females (9.4 mu mol/L). A significant difference (p=0.031) was observed between the incidences of HHcy in healthy males (44.0%) and healthy females (16.0%). We conclude that the incidence of hyperhomocysteinemia and homocysteinemia are significantly higher in all the patients compared with de healthy persons, as well as in both healthy males and males with PTE compared with healthy females and female patients. This indicates that HHcy findings in PE are likely to have a clinical importance.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Hyperhomocysteinemia in patients with pulmonary embolism",
volume = "62",
number = "4",
pages = "907-914",
doi = "10.2298/ABS1004907R"
}

Radovanović, N., Antonijević, N., Beletić, A., Perunicić, J., Kocica, M. J., Mirković, D., Lacković, V.,& Lacković, M.. (2010). Hyperhomocysteinemia in patients with pulmonary embolism. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 907-914.
https://doi.org/10.2298/ABS1004907R

Radovanović N, Antonijević N, Beletić A, Perunicić J, Kocica MJ, Mirković D, Lacković V, Lacković M. Hyperhomocysteinemia in patients with pulmonary embolism. in Archives of Biological Sciences. 2010;62(4):907-914.
doi:10.2298/ABS1004907R .

Radovanović, N., Antonijević, N., Beletić, Anđelo, Perunicić, J., Kocica, Mladen J., Mirković, Duško, Lacković, Vesna, Lacković, Milena, "Hyperhomocysteinemia in patients with pulmonary embolism" in Archives of Biological Sciences, 62, no. 4 (2010):907-914,
https://doi.org/10.2298/ABS1004907R . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Đorđević, Valentina
AU  - Dudvarski-Ilić, Aleksandra
AU  - Obradović, Ivana
AU  - Mirković, Duško
AU  - Ilić, Mirka
AU  - Radojković, Dragica
AU  - Majkić-Singh, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1163
AB  - Alpha-1-antitrypsin deficiency is a potentially lethal genetic disorder, which has pulmonary and liver manifestations. The standardized biochemical and molecular diagnostic protocol for detection of clinically relevant alleles is needed. The paper summarizes current concepts about AATD, describes the potentials of isoelectric focusing and PCR amplification-reverse allele specific oligonucleotide hybridization assay in the detection of affected individuals and shortly presents our experiences in the evaluation of AATD. We conclude that the systematic clinical laboratory approach to AATD might be based on the combination of mentioned methods, coordinated by alpha-1-antritrypsin quantification. Additionally, its complete medical implementation is achieved through teamwork between clinical chemists, molecular biologists and clinicians.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency
VL  - 28
IS  - 4
SP  - 241
EP  - 247
DO  - 10.2478/v10011-009-0023-x
ER  - 

@article{
author = "Beletić, Anđelo and Đorđević, Valentina and Dudvarski-Ilić, Aleksandra and Obradović, Ivana and Mirković, Duško and Ilić, Mirka and Radojković, Dragica and Majkić-Singh, Nada",
year = "2009",
abstract = "Alpha-1-antitrypsin deficiency is a potentially lethal genetic disorder, which has pulmonary and liver manifestations. The standardized biochemical and molecular diagnostic protocol for detection of clinically relevant alleles is needed. The paper summarizes current concepts about AATD, describes the potentials of isoelectric focusing and PCR amplification-reverse allele specific oligonucleotide hybridization assay in the detection of affected individuals and shortly presents our experiences in the evaluation of AATD. We conclude that the systematic clinical laboratory approach to AATD might be based on the combination of mentioned methods, coordinated by alpha-1-antritrypsin quantification. Additionally, its complete medical implementation is achieved through teamwork between clinical chemists, molecular biologists and clinicians.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency",
volume = "28",
number = "4",
pages = "241-247",
doi = "10.2478/v10011-009-0023-x"
}

Beletić, A., Đorđević, V., Dudvarski-Ilić, A., Obradović, I., Mirković, D., Ilić, M., Radojković, D.,& Majkić-Singh, N.. (2009). Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 28(4), 241-247.
https://doi.org/10.2478/v10011-009-0023-x

Beletić A, Đorđević V, Dudvarski-Ilić A, Obradović I, Mirković D, Ilić M, Radojković D, Majkić-Singh N. Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency. in Journal of Medical Biochemistry. 2009;28(4):241-247.
doi:10.2478/v10011-009-0023-x .

Beletić, Anđelo, Đorđević, Valentina, Dudvarski-Ilić, Aleksandra, Obradović, Ivana, Mirković, Duško, Ilić, Mirka, Radojković, Dragica, Majkić-Singh, Nada, "Isoelectrofocusing and PCR Amplification-Reverse Hybridization Assay in Evaluation of Alpha-1-Antitrypsin Deficiency" in Journal of Medical Biochemistry, 28, no. 4 (2009):241-247,
https://doi.org/10.2478/v10011-009-0023-x . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Majkić-Singh, Nada
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Pejanović, Svetlana D.
AU  - Vujanić, Svetlana T.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1189
AB  - Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients
VL  - 55
IS  - 9-10
SP  - 333
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1189
ER  - 

@article{
author = "Milinković, Neda and Majkić-Singh, Nada and Mirković, Duško and Beletić, Anđelo and Pejanović, Svetlana D. and Vujanić, Svetlana T.",
year = "2009",
abstract = "Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients",
volume = "55",
number = "9-10",
pages = "333-339",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1189"
}

Milinković, N., Majkić-Singh, N., Mirković, D., Beletić, A., Pejanović, S. D.,& Vujanić, S. T.. (2009). Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 55(9-10), 333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189

Milinković N, Majkić-Singh N, Mirković D, Beletić A, Pejanović SD, Vujanić ST. Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory. 2009;55(9-10):333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189 .

Milinković, Neda, Majkić-Singh, Nada, Mirković, Duško, Beletić, Anđelo, Pejanović, Svetlana D., Vujanić, Svetlana T., "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients" in Clinical Laboratory, 55, no. 9-10 (2009):333-339,
https://hdl.handle.net/21.15107/rcub_farfar_1189 .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Antonijević, Nebojša
AU  - Đorđević, Valentina
AU  - Sango, Violeta
AU  - Jakovljević, Branko
AU  - Perunicić, Jovan
AU  - Ilić, Mirka
AU  - Vasiljević, Zorana
AU  - Majkić-Singh, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1168
AB  - Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylenetetra-hydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 mu mol/L), homocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P = 0.038). Median homocysteine levels in patients (10.4 mu mol/L) and controls (9.6 mu mol/L) were significantly different (P=0.035). Among patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from the observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Incidence of hyperhomocysteinemia and Mthfr C677T polymorphism among young patients with acute myocardial infarction
VL  - 28
IS  - 1
SP  - 41
EP  - 45
DO  - 10.2478/v10011-008-0029-9
ER  - 

@article{
author = "Beletić, Anđelo and Mirković, Duško and Antonijević, Nebojša and Đorđević, Valentina and Sango, Violeta and Jakovljević, Branko and Perunicić, Jovan and Ilić, Mirka and Vasiljević, Zorana and Majkić-Singh, Nada",
year = "2009",
abstract = "Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylenetetra-hydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 mu mol/L), homocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P = 0.038). Median homocysteine levels in patients (10.4 mu mol/L) and controls (9.6 mu mol/L) were significantly different (P=0.035). Among patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from the observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Incidence of hyperhomocysteinemia and Mthfr C677T polymorphism among young patients with acute myocardial infarction",
volume = "28",
number = "1",
pages = "41-45",
doi = "10.2478/v10011-008-0029-9"
}

Beletić, A., Mirković, D., Antonijević, N., Đorđević, V., Sango, V., Jakovljević, B., Perunicić, J., Ilić, M., Vasiljević, Z.,& Majkić-Singh, N.. (2009). Incidence of hyperhomocysteinemia and Mthfr C677T polymorphism among young patients with acute myocardial infarction. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 28(1), 41-45.
https://doi.org/10.2478/v10011-008-0029-9

Beletić A, Mirković D, Antonijević N, Đorđević V, Sango V, Jakovljević B, Perunicić J, Ilić M, Vasiljević Z, Majkić-Singh N. Incidence of hyperhomocysteinemia and Mthfr C677T polymorphism among young patients with acute myocardial infarction. in Journal of Medical Biochemistry. 2009;28(1):41-45.
doi:10.2478/v10011-008-0029-9 .

Beletić, Anđelo, Mirković, Duško, Antonijević, Nebojša, Đorđević, Valentina, Sango, Violeta, Jakovljević, Branko, Perunicić, Jovan, Ilić, Mirka, Vasiljević, Zorana, Majkić-Singh, Nada, "Incidence of hyperhomocysteinemia and Mthfr C677T polymorphism among young patients with acute myocardial infarction" in Journal of Medical Biochemistry, 28, no. 1 (2009):41-45,
https://doi.org/10.2478/v10011-008-0029-9 . .