TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
AU  - Dopsaj, Milivoj
AU  - Kasum, Goran
AU  - Kotur-Stevuljević, Jelena
AU  - Koropanovski, Nenad
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1900
AB  - The aim of this study was to profile hematological, oxidative stress, and immunological parameters in male athletes who practiced combat sports and to determine whether the type of combat sport influenced the measured parameters. Eighteen karate professionals, 15 wrestlers, and 14 kickboxers participated in the study. Hematological, iron-related, oxidative stress, and immunological parameters were measured at the beginning of a precompetitive period. The general linear model showed significant differences between the karate professionals, wrestlers, and kickboxers with respect to their hematological and iron status parameters (Wilks' Lambda = 0.270, F = 2.186, p  lt  0.05) and oxidative stress status (Wilks' Lambda = 0.529, F = 1.940, p  lt  0.05). The immature reticulocyte fraction was significantly higher in wrestlers (0.30 +/- 0.03) compared with kickboxers (0.24 +/- 0.04; p  lt  0.05) and karate professionals (0.26 +/- 0.04; p  lt  0.05). Low hemoglobin density was significantly lower in wrestlers and kickboxers (p  lt  0.05) compared with karate professionals (karate: 3.51 +/- 1.19, wrestlers: 1.95 +/- 1.10, and kickboxers: 1.77 +/- 0.76). Significant differences were observed between the karate professionals and wrestlers with respect to their pro-oxidant-antioxidant balance (437 +/- 103 vs. 323 +/- 148, p  lt  0.05) and superoxide-dismutase activity (SOD) (73 +/- 37 vs. 103 +/- 30, p  lt  0.05). All the measured parameters (with the exception of SOD activity) fell within their physiological ranges, indicating that the study participants represented a young and healthy male population. Hematological parameters differed between kickboxers and karate professionals. The low pro-oxidant-antioxidant balance and high SOD activity in wrestlers could be associated with the long-term impact of wrestling as a type of strenuous exercise.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Strength and Conditioning Research
T1  - Hematological, oxidative stress, and immune status profiling in elite combat sport athletes
VL  - 27
IS  - 12
SP  - 3506
EP  - 3514
DO  - 10.1519/JSC.0b013e31828ddeea
ER  - 

@article{
author = "Dopsaj, Violeta and Martinović, Jelena and Dopsaj, Milivoj and Kasum, Goran and Kotur-Stevuljević, Jelena and Koropanovski, Nenad",
year = "2013",
abstract = "The aim of this study was to profile hematological, oxidative stress, and immunological parameters in male athletes who practiced combat sports and to determine whether the type of combat sport influenced the measured parameters. Eighteen karate professionals, 15 wrestlers, and 14 kickboxers participated in the study. Hematological, iron-related, oxidative stress, and immunological parameters were measured at the beginning of a precompetitive period. The general linear model showed significant differences between the karate professionals, wrestlers, and kickboxers with respect to their hematological and iron status parameters (Wilks' Lambda = 0.270, F = 2.186, p  lt  0.05) and oxidative stress status (Wilks' Lambda = 0.529, F = 1.940, p  lt  0.05). The immature reticulocyte fraction was significantly higher in wrestlers (0.30 +/- 0.03) compared with kickboxers (0.24 +/- 0.04; p  lt  0.05) and karate professionals (0.26 +/- 0.04; p  lt  0.05). Low hemoglobin density was significantly lower in wrestlers and kickboxers (p  lt  0.05) compared with karate professionals (karate: 3.51 +/- 1.19, wrestlers: 1.95 +/- 1.10, and kickboxers: 1.77 +/- 0.76). Significant differences were observed between the karate professionals and wrestlers with respect to their pro-oxidant-antioxidant balance (437 +/- 103 vs. 323 +/- 148, p  lt  0.05) and superoxide-dismutase activity (SOD) (73 +/- 37 vs. 103 +/- 30, p  lt  0.05). All the measured parameters (with the exception of SOD activity) fell within their physiological ranges, indicating that the study participants represented a young and healthy male population. Hematological parameters differed between kickboxers and karate professionals. The low pro-oxidant-antioxidant balance and high SOD activity in wrestlers could be associated with the long-term impact of wrestling as a type of strenuous exercise.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Strength and Conditioning Research",
title = "Hematological, oxidative stress, and immune status profiling in elite combat sport athletes",
volume = "27",
number = "12",
pages = "3506-3514",
doi = "10.1519/JSC.0b013e31828ddeea"
}

Dopsaj, V., Martinović, J., Dopsaj, M., Kasum, G., Kotur-Stevuljević, J.,& Koropanovski, N.. (2013). Hematological, oxidative stress, and immune status profiling in elite combat sport athletes. in Journal of Strength and Conditioning Research
Lippincott Williams & Wilkins, Philadelphia., 27(12), 3506-3514.
https://doi.org/10.1519/JSC.0b013e31828ddeea

Dopsaj V, Martinović J, Dopsaj M, Kasum G, Kotur-Stevuljević J, Koropanovski N. Hematological, oxidative stress, and immune status profiling in elite combat sport athletes. in Journal of Strength and Conditioning Research. 2013;27(12):3506-3514.
doi:10.1519/JSC.0b013e31828ddeea .

Dopsaj, Violeta, Martinović, Jelena, Dopsaj, Milivoj, Kasum, Goran, Kotur-Stevuljević, Jelena, Koropanovski, Nenad, "Hematological, oxidative stress, and immune status profiling in elite combat sport athletes" in Journal of Strength and Conditioning Research, 27, no. 12 (2013):3506-3514,
https://doi.org/10.1519/JSC.0b013e31828ddeea . .

TY  - CHAP
AU  - Martinović, J
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Dopsaj, Milivoj
AU  - Kasum, Goran
AU  - Nešić, G
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1808
AB  - Physical activity has been shown to increase the production of free radicals to a point that can exceed internal protective antioxidant system. The harmful effects of free radicals are neutralized by activity of antioxidant enzymes and numerous non-enzymatic antioxidants, including vitamins, glutathione, ubiquinone and flavonoids. Defence mechanisms against free radical-mediated oxidative damage also include the iron binding proteins such as transferrin and ferritin. Ferritin and trensferrin are able to restrict the availability of iron to participate in conversion of hydrogen peroxide to toxic hydroxyl radicals by Fenton reaction. Because the endogenous antioxidant system may not be sufficient to prevent exercise-induced free radical generation and consequent oxidative damage, supplementation of antioxidants may have important effect antioxidant status in athletes. The aim of the present work was to examine the association of physiological iron carriers with oxidative stress in elite athletes. One hundred and five elite athletes were divided into two groups: supplemented group which consisted of sixty-five athletes who regularly use antioxidant supplements and control group which consisted of forty athletes that were without antioxidants intake. Following parameters were measured: albumin, ferritin, transferrin, advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), superoxide dismutase (SOD) and total sulphydryl group concentration (SH groups). Multivariate analysis of covariance (MANCOVA, Wilks' lambda) was performed to test the hypotheses that supplementation (fixed factor) and antioxidative proteins (indicated via albumin, ferritin and transferrin) (covariates) have a significant effect on the oxidative stress parameters (dependent variables). The SOD activity (P=0.024) and sulphydryl group concentration (P=0.042) were significantly higher in supplemented athletes. Multivariate analysis of covariance indicated ferritin (P<0.001) and transferrin (P=0.001) as significant covariates, which have contributed 37.1 % and 22.8% to variability of oxidative stres parameters, respectively. The transferrin exhibited linear relationships with LOOH (R2 = 0.127; P < 0.001) and AOPP (R2 = 0.113; P < 0.001) while ferritin exhibited non-linear (logarithmic) relationships with this parameters (AOPP: R2 = 0.201, y = 82.3 -15.9log(x), P<0.001; LOOH: R2 = 0.256, y = 193 -33.3log(x), P<0.001). In conclusion, proteins that regulate iron transport and storage, transferrin and ferritin, were negatively related with oxidative damage in professional athletes regardless of antioxidant supplementation.
PB  - Nova Science Publishers, Inc.
T2  - Ferritin: Functions, Biosynthesis and Regulation
T1  - The antioxidant role of ferritin and transferrin in elite athletes
SP  - 101
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1808
ER  - 

@inbook{
author = "Martinović, J and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Dopsaj, Milivoj and Kasum, Goran and Nešić, G",
year = "2012",
abstract = "Physical activity has been shown to increase the production of free radicals to a point that can exceed internal protective antioxidant system. The harmful effects of free radicals are neutralized by activity of antioxidant enzymes and numerous non-enzymatic antioxidants, including vitamins, glutathione, ubiquinone and flavonoids. Defence mechanisms against free radical-mediated oxidative damage also include the iron binding proteins such as transferrin and ferritin. Ferritin and trensferrin are able to restrict the availability of iron to participate in conversion of hydrogen peroxide to toxic hydroxyl radicals by Fenton reaction. Because the endogenous antioxidant system may not be sufficient to prevent exercise-induced free radical generation and consequent oxidative damage, supplementation of antioxidants may have important effect antioxidant status in athletes. The aim of the present work was to examine the association of physiological iron carriers with oxidative stress in elite athletes. One hundred and five elite athletes were divided into two groups: supplemented group which consisted of sixty-five athletes who regularly use antioxidant supplements and control group which consisted of forty athletes that were without antioxidants intake. Following parameters were measured: albumin, ferritin, transferrin, advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), superoxide dismutase (SOD) and total sulphydryl group concentration (SH groups). Multivariate analysis of covariance (MANCOVA, Wilks' lambda) was performed to test the hypotheses that supplementation (fixed factor) and antioxidative proteins (indicated via albumin, ferritin and transferrin) (covariates) have a significant effect on the oxidative stress parameters (dependent variables). The SOD activity (P=0.024) and sulphydryl group concentration (P=0.042) were significantly higher in supplemented athletes. Multivariate analysis of covariance indicated ferritin (P<0.001) and transferrin (P=0.001) as significant covariates, which have contributed 37.1 % and 22.8% to variability of oxidative stres parameters, respectively. The transferrin exhibited linear relationships with LOOH (R2 = 0.127; P < 0.001) and AOPP (R2 = 0.113; P < 0.001) while ferritin exhibited non-linear (logarithmic) relationships with this parameters (AOPP: R2 = 0.201, y = 82.3 -15.9log(x), P<0.001; LOOH: R2 = 0.256, y = 193 -33.3log(x), P<0.001). In conclusion, proteins that regulate iron transport and storage, transferrin and ferritin, were negatively related with oxidative damage in professional athletes regardless of antioxidant supplementation.",
publisher = "Nova Science Publishers, Inc.",
journal = "Ferritin: Functions, Biosynthesis and Regulation",
booktitle = "The antioxidant role of ferritin and transferrin in elite athletes",
pages = "101-118",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1808"
}

Martinović, J., Dopsaj, V., Kotur-Stevuljević, J., Dopsaj, M., Kasum, G.,& Nešić, G.. (2012). The antioxidant role of ferritin and transferrin in elite athletes. in Ferritin: Functions, Biosynthesis and Regulation
Nova Science Publishers, Inc.., 101-118.
https://hdl.handle.net/21.15107/rcub_farfar_1808

Martinović J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Kasum G, Nešić G. The antioxidant role of ferritin and transferrin in elite athletes. in Ferritin: Functions, Biosynthesis and Regulation. 2012;:101-118.
https://hdl.handle.net/21.15107/rcub_farfar_1808 .

Martinović, J, Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Dopsaj, Milivoj, Kasum, Goran, Nešić, G, "The antioxidant role of ferritin and transferrin in elite athletes" in Ferritin: Functions, Biosynthesis and Regulation (2012):101-118,
https://hdl.handle.net/21.15107/rcub_farfar_1808 .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Dopsaj, Milivoj
AU  - Stefanović, Aleksandra
AU  - Kasum, Goran
AU  - Vujović, Ana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1409
AB  - The main purpose of this study was to demonstrate the impact of different training regimes and the type of metabolism that predominates as the source of energy on the oxidative stress / anti-oxidative defense status in elite-level trained athletes. One hundred twenty-four athletes were divided into three groups: 'aerobic' (karate, rowing, triathlon), 'anaerobic' (wrestling and swimming) and 'mixed' (volleyball, water polo, kick boxing). The following parameters were measured: oxidative stress status parameters: [(reactive oxygen metabolites (ROMs), superoxide anion (O2 -), malondialdehyde (MDA), advanced oxidation protein products (AOPP) and lipid hydroperoxides (LOOH)] and anti-oxidative defense parameters [biological anti-oxidative potential (BAP), superoxide-dismutase (SOD), sulphydryl groups (-SH) and pro-oxidant-antioxidant balance (PAB)]. In general, significant differences were found in oxidative stress parameters between three experimental groups (Wilks' Lambda = 0.366, F value = 8.185, p  lt  0.001). Comparing the athletes from the anaerobic and mixed groups, we found that the anaerobic group had significantly lower ROMs (p = 0.019), AOPP (p  lt  0.001), O2 - (p = 0.003) and LOOH (p  lt  0.001). The aerobic group of athletes differed from the group with mixed training regime by lower AOPP, MDA, O2 - and LOOH values (p  lt  0.001). Discriminant analysis of the three experimental groups indicated protein oxidation marker (AOPP) and pro-oxidant-antioxidant balance as the most important discriminant variables. Oxidative stress status parameters adequately discriminated 74.2 % of the athletes with different energy expenditure during their training programs. The results obtained provide evidence that there are differences in the oxidative stress / anti-oxidant defense status between athletes that have different energy expenditure during exercise and identify athletes who participate in team sports as most susceptible to oxidative stress. .
PB  - Sportska akademija, Beograd
T2  - Serbian Journal of Sports sciences
T1  - Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?
VL  - 4
IS  - 1-4
SP  - 75
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1409
ER  - 

@article{
author = "Martinović, Jelena and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Dopsaj, Milivoj and Stefanović, Aleksandra and Kasum, Goran and Vujović, Ana",
year = "2010",
abstract = "The main purpose of this study was to demonstrate the impact of different training regimes and the type of metabolism that predominates as the source of energy on the oxidative stress / anti-oxidative defense status in elite-level trained athletes. One hundred twenty-four athletes were divided into three groups: 'aerobic' (karate, rowing, triathlon), 'anaerobic' (wrestling and swimming) and 'mixed' (volleyball, water polo, kick boxing). The following parameters were measured: oxidative stress status parameters: [(reactive oxygen metabolites (ROMs), superoxide anion (O2 -), malondialdehyde (MDA), advanced oxidation protein products (AOPP) and lipid hydroperoxides (LOOH)] and anti-oxidative defense parameters [biological anti-oxidative potential (BAP), superoxide-dismutase (SOD), sulphydryl groups (-SH) and pro-oxidant-antioxidant balance (PAB)]. In general, significant differences were found in oxidative stress parameters between three experimental groups (Wilks' Lambda = 0.366, F value = 8.185, p  lt  0.001). Comparing the athletes from the anaerobic and mixed groups, we found that the anaerobic group had significantly lower ROMs (p = 0.019), AOPP (p  lt  0.001), O2 - (p = 0.003) and LOOH (p  lt  0.001). The aerobic group of athletes differed from the group with mixed training regime by lower AOPP, MDA, O2 - and LOOH values (p  lt  0.001). Discriminant analysis of the three experimental groups indicated protein oxidation marker (AOPP) and pro-oxidant-antioxidant balance as the most important discriminant variables. Oxidative stress status parameters adequately discriminated 74.2 % of the athletes with different energy expenditure during their training programs. The results obtained provide evidence that there are differences in the oxidative stress / anti-oxidant defense status between athletes that have different energy expenditure during exercise and identify athletes who participate in team sports as most susceptible to oxidative stress. .",
publisher = "Sportska akademija, Beograd",
journal = "Serbian Journal of Sports sciences",
title = "Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?",
volume = "4",
number = "1-4",
pages = "75-81",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1409"
}

Martinović, J., Dopsaj, V., Kotur-Stevuljević, J., Dopsaj, M., Stefanović, A., Kasum, G.,& Vujović, A.. (2010). Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?. in Serbian Journal of Sports sciences
Sportska akademija, Beograd., 4(1-4), 75-81.
https://hdl.handle.net/21.15107/rcub_farfar_1409

Martinović J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Stefanović A, Kasum G, Vujović A. Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?. in Serbian Journal of Sports sciences. 2010;4(1-4):75-81.
https://hdl.handle.net/21.15107/rcub_farfar_1409 .

Martinović, Jelena, Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Dopsaj, Milivoj, Stefanović, Aleksandra, Kasum, Goran, Vujović, Ana, "Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?" in Serbian Journal of Sports sciences, 4, no. 1-4 (2010):75-81,
https://hdl.handle.net/21.15107/rcub_farfar_1409 .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Kotur-Stevuljević, Jelena
AU  - Dopsaj, Violeta
AU  - Dopsaj, Milivoj
AU  - Stefanović, Aleksandra
AU  - Kasum, Goran
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1368
AB  - Objective: The aim of this study was to evaluate how conditions that precede anaemia (iron store depletion and iron-deficient erythropoiesis) affect human serum paraoxonase PON1 activity. Design and methods: Based on haemoglobin, transferrin saturation and serum ferritin values 119 athletes were divided into three groups: with iron depletion, with deficient erythropoiesis and controls. The following parameters were measured: paraoxonase activity towards paraoxon (POase) and diazoxon (DZOase), lipid hydroperoxides (LOOH), the pro-oxidant-antioxidant balance (PAB), red blood cells (RBC) and lipid status. Results: Significant differences were found between athletes with different stages of iron deficiency and controls with respect to PON 1 activity and oxidative stress status parameters (Wilks' Lambda = 0.712, F = 5.241, p lt 0.001, eta(2) = 0.156). There was no significant difference between the PON1 192 Q and R polymorphism distribution in the two groups of athletes with different stages of iron deficiency and controls (chi(2) = 1.086; p = 0.896). PON1 activity was positively correlated with RBCs, haemoglobin, transferrin saturation (p lt 0.001) and ferritin (p = 0.037) and negatively correlated with LOOH (p = 0.044) in all three study groups. Conclusions: Deficient erythropoiesis in athletes contributes to impaired PON1 activity. In contrast, iron depletion, regardless of increased oxidative stress, does not affect PON1 activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis
VL  - 43
IS  - 15
SP  - 1225
EP  - 1229
DO  - 10.1016/j.clinbiochem.2010.07.024
ER  - 

@article{
author = "Martinović, Jelena and Kotur-Stevuljević, Jelena and Dopsaj, Violeta and Dopsaj, Milivoj and Stefanović, Aleksandra and Kasum, Goran",
year = "2010",
abstract = "Objective: The aim of this study was to evaluate how conditions that precede anaemia (iron store depletion and iron-deficient erythropoiesis) affect human serum paraoxonase PON1 activity. Design and methods: Based on haemoglobin, transferrin saturation and serum ferritin values 119 athletes were divided into three groups: with iron depletion, with deficient erythropoiesis and controls. The following parameters were measured: paraoxonase activity towards paraoxon (POase) and diazoxon (DZOase), lipid hydroperoxides (LOOH), the pro-oxidant-antioxidant balance (PAB), red blood cells (RBC) and lipid status. Results: Significant differences were found between athletes with different stages of iron deficiency and controls with respect to PON 1 activity and oxidative stress status parameters (Wilks' Lambda = 0.712, F = 5.241, p lt 0.001, eta(2) = 0.156). There was no significant difference between the PON1 192 Q and R polymorphism distribution in the two groups of athletes with different stages of iron deficiency and controls (chi(2) = 1.086; p = 0.896). PON1 activity was positively correlated with RBCs, haemoglobin, transferrin saturation (p lt 0.001) and ferritin (p = 0.037) and negatively correlated with LOOH (p = 0.044) in all three study groups. Conclusions: Deficient erythropoiesis in athletes contributes to impaired PON1 activity. In contrast, iron depletion, regardless of increased oxidative stress, does not affect PON1 activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis",
volume = "43",
number = "15",
pages = "1225-1229",
doi = "10.1016/j.clinbiochem.2010.07.024"
}

Martinović, J., Kotur-Stevuljević, J., Dopsaj, V., Dopsaj, M., Stefanović, A.,& Kasum, G.. (2010). Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 43(15), 1225-1229.
https://doi.org/10.1016/j.clinbiochem.2010.07.024

Martinović J, Kotur-Stevuljević J, Dopsaj V, Dopsaj M, Stefanović A, Kasum G. Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis. in Clinical Biochemistry. 2010;43(15):1225-1229.
doi:10.1016/j.clinbiochem.2010.07.024 .

Martinović, Jelena, Kotur-Stevuljević, Jelena, Dopsaj, Violeta, Dopsaj, Milivoj, Stefanović, Aleksandra, Kasum, Goran, "Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis" in Clinical Biochemistry, 43, no. 15 (2010):1225-1229,
https://doi.org/10.1016/j.clinbiochem.2010.07.024 . .