TY  - CHAP
AU  - Martinović, J
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Dopsaj, Milivoj
AU  - Kasum, Goran
AU  - Nešić, G
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1808
AB  - Physical activity has been shown to increase the production of free radicals to a point that can exceed internal protective antioxidant system. The harmful effects of free radicals are neutralized by activity of antioxidant enzymes and numerous non-enzymatic antioxidants, including vitamins, glutathione, ubiquinone and flavonoids. Defence mechanisms against free radical-mediated oxidative damage also include the iron binding proteins such as transferrin and ferritin. Ferritin and trensferrin are able to restrict the availability of iron to participate in conversion of hydrogen peroxide to toxic hydroxyl radicals by Fenton reaction. Because the endogenous antioxidant system may not be sufficient to prevent exercise-induced free radical generation and consequent oxidative damage, supplementation of antioxidants may have important effect antioxidant status in athletes. The aim of the present work was to examine the association of physiological iron carriers with oxidative stress in elite athletes. One hundred and five elite athletes were divided into two groups: supplemented group which consisted of sixty-five athletes who regularly use antioxidant supplements and control group which consisted of forty athletes that were without antioxidants intake. Following parameters were measured: albumin, ferritin, transferrin, advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), superoxide dismutase (SOD) and total sulphydryl group concentration (SH groups). Multivariate analysis of covariance (MANCOVA, Wilks' lambda) was performed to test the hypotheses that supplementation (fixed factor) and antioxidative proteins (indicated via albumin, ferritin and transferrin) (covariates) have a significant effect on the oxidative stress parameters (dependent variables). The SOD activity (P=0.024) and sulphydryl group concentration (P=0.042) were significantly higher in supplemented athletes. Multivariate analysis of covariance indicated ferritin (P<0.001) and transferrin (P=0.001) as significant covariates, which have contributed 37.1 % and 22.8% to variability of oxidative stres parameters, respectively. The transferrin exhibited linear relationships with LOOH (R2 = 0.127; P < 0.001) and AOPP (R2 = 0.113; P < 0.001) while ferritin exhibited non-linear (logarithmic) relationships with this parameters (AOPP: R2 = 0.201, y = 82.3 -15.9log(x), P<0.001; LOOH: R2 = 0.256, y = 193 -33.3log(x), P<0.001). In conclusion, proteins that regulate iron transport and storage, transferrin and ferritin, were negatively related with oxidative damage in professional athletes regardless of antioxidant supplementation.
PB  - Nova Science Publishers, Inc.
T2  - Ferritin: Functions, Biosynthesis and Regulation
T1  - The antioxidant role of ferritin and transferrin in elite athletes
SP  - 101
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1808
ER  - 

@inbook{
author = "Martinović, J and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Dopsaj, Milivoj and Kasum, Goran and Nešić, G",
year = "2012",
abstract = "Physical activity has been shown to increase the production of free radicals to a point that can exceed internal protective antioxidant system. The harmful effects of free radicals are neutralized by activity of antioxidant enzymes and numerous non-enzymatic antioxidants, including vitamins, glutathione, ubiquinone and flavonoids. Defence mechanisms against free radical-mediated oxidative damage also include the iron binding proteins such as transferrin and ferritin. Ferritin and trensferrin are able to restrict the availability of iron to participate in conversion of hydrogen peroxide to toxic hydroxyl radicals by Fenton reaction. Because the endogenous antioxidant system may not be sufficient to prevent exercise-induced free radical generation and consequent oxidative damage, supplementation of antioxidants may have important effect antioxidant status in athletes. The aim of the present work was to examine the association of physiological iron carriers with oxidative stress in elite athletes. One hundred and five elite athletes were divided into two groups: supplemented group which consisted of sixty-five athletes who regularly use antioxidant supplements and control group which consisted of forty athletes that were without antioxidants intake. Following parameters were measured: albumin, ferritin, transferrin, advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), superoxide dismutase (SOD) and total sulphydryl group concentration (SH groups). Multivariate analysis of covariance (MANCOVA, Wilks' lambda) was performed to test the hypotheses that supplementation (fixed factor) and antioxidative proteins (indicated via albumin, ferritin and transferrin) (covariates) have a significant effect on the oxidative stress parameters (dependent variables). The SOD activity (P=0.024) and sulphydryl group concentration (P=0.042) were significantly higher in supplemented athletes. Multivariate analysis of covariance indicated ferritin (P<0.001) and transferrin (P=0.001) as significant covariates, which have contributed 37.1 % and 22.8% to variability of oxidative stres parameters, respectively. The transferrin exhibited linear relationships with LOOH (R2 = 0.127; P < 0.001) and AOPP (R2 = 0.113; P < 0.001) while ferritin exhibited non-linear (logarithmic) relationships with this parameters (AOPP: R2 = 0.201, y = 82.3 -15.9log(x), P<0.001; LOOH: R2 = 0.256, y = 193 -33.3log(x), P<0.001). In conclusion, proteins that regulate iron transport and storage, transferrin and ferritin, were negatively related with oxidative damage in professional athletes regardless of antioxidant supplementation.",
publisher = "Nova Science Publishers, Inc.",
journal = "Ferritin: Functions, Biosynthesis and Regulation",
booktitle = "The antioxidant role of ferritin and transferrin in elite athletes",
pages = "101-118",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1808"
}

Martinović, J., Dopsaj, V., Kotur-Stevuljević, J., Dopsaj, M., Kasum, G.,& Nešić, G.. (2012). The antioxidant role of ferritin and transferrin in elite athletes. in Ferritin: Functions, Biosynthesis and Regulation
Nova Science Publishers, Inc.., 101-118.
https://hdl.handle.net/21.15107/rcub_farfar_1808

Martinović J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Kasum G, Nešić G. The antioxidant role of ferritin and transferrin in elite athletes. in Ferritin: Functions, Biosynthesis and Regulation. 2012;:101-118.
https://hdl.handle.net/21.15107/rcub_farfar_1808 .

Martinović, J, Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Dopsaj, Milivoj, Kasum, Goran, Nešić, G, "The antioxidant role of ferritin and transferrin in elite athletes" in Ferritin: Functions, Biosynthesis and Regulation (2012):101-118,
https://hdl.handle.net/21.15107/rcub_farfar_1808 .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Martinović, J.
AU  - Dopsaj, Milivoj
AU  - Kotur-Stevuljević, Jelena
AU  - Bogavac-Stanojević, Nataša
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1539
AB  - The aim of the present study was to examine the association of proteins that regulate iron transport/storage content and acute phase response with oxidative stress in male and female athletes. Serum ferritin, transferrin, soluble transferrin receptor, C-reactive protein, interleukin-6 and oxidative stress parameters (reactive oxygen metabolites, superoxide anion, advanced oxidation protein products, lipid hydroperoxides, superoxide-dismutase and pro-oxidant-antioxidant balance) were determined in 138 athletes (73 females and 65 males). A general linear model indicated significant gender differences between athletes in terms of reactive oxygen metabolites (307.48 +/- 61.02 vs. 276.98 +/- 50.08; P = 0.030), superoxide-dismutase (114.60 +/- 41.64 vs. 101.42 +/- 38.76; P = 0.001), lipid hydroperoxides (149.84 +/- 38.95 vs. 101.43 +/- 39.26; P  lt  0.001), pro-oxidant-antioxidant balance (512.40 +/- 148.67 vs. 413.09 +/- 120.30; P = 0.002), advanced oxidation protein products (1.49 +/- 0.30 vs. 0.91 +/- 0.25; P  lt  0.001) and superoxide (2.61 +/- 0.36 vs. 2.22 +/- 0.35; P = 0.001), which were all significantly higher in females. Multivariate analysis of covariance indicated gender (P  lt  0.001), training experience (P = 0.004), Creactive protein (P = 0.002), soluble transferrin receptor (P = 0.004) and transferrin (P  lt  0.001) as significant covariates. Gender accounted for the largest proportion of variability for all oxidative stress parameters (46.3%) and female athletes were more susceptible to oxidative stress. Iron transport and storage proteins (transferrin and ferritin), but also acute phase reactants, were negatively related factors for oxidative stress. In conclusion, variation in the ferritin level may contribute to the different oxidative stress level between the sexes.
PB  - Georg Thieme Verlag Kg, Stuttgart
T2  - International Journal of Sports Medicine
T1  - Gender-Specific Oxidative Stress Parameters
VL  - 32
IS  - 1
SP  - 14
EP  - 19
DO  - 10.1055/s-0030-1267930
ER  - 

@article{
author = "Dopsaj, Violeta and Martinović, J. and Dopsaj, Milivoj and Kotur-Stevuljević, Jelena and Bogavac-Stanojević, Nataša",
year = "2011",
abstract = "The aim of the present study was to examine the association of proteins that regulate iron transport/storage content and acute phase response with oxidative stress in male and female athletes. Serum ferritin, transferrin, soluble transferrin receptor, C-reactive protein, interleukin-6 and oxidative stress parameters (reactive oxygen metabolites, superoxide anion, advanced oxidation protein products, lipid hydroperoxides, superoxide-dismutase and pro-oxidant-antioxidant balance) were determined in 138 athletes (73 females and 65 males). A general linear model indicated significant gender differences between athletes in terms of reactive oxygen metabolites (307.48 +/- 61.02 vs. 276.98 +/- 50.08; P = 0.030), superoxide-dismutase (114.60 +/- 41.64 vs. 101.42 +/- 38.76; P = 0.001), lipid hydroperoxides (149.84 +/- 38.95 vs. 101.43 +/- 39.26; P  lt  0.001), pro-oxidant-antioxidant balance (512.40 +/- 148.67 vs. 413.09 +/- 120.30; P = 0.002), advanced oxidation protein products (1.49 +/- 0.30 vs. 0.91 +/- 0.25; P  lt  0.001) and superoxide (2.61 +/- 0.36 vs. 2.22 +/- 0.35; P = 0.001), which were all significantly higher in females. Multivariate analysis of covariance indicated gender (P  lt  0.001), training experience (P = 0.004), Creactive protein (P = 0.002), soluble transferrin receptor (P = 0.004) and transferrin (P  lt  0.001) as significant covariates. Gender accounted for the largest proportion of variability for all oxidative stress parameters (46.3%) and female athletes were more susceptible to oxidative stress. Iron transport and storage proteins (transferrin and ferritin), but also acute phase reactants, were negatively related factors for oxidative stress. In conclusion, variation in the ferritin level may contribute to the different oxidative stress level between the sexes.",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "International Journal of Sports Medicine",
title = "Gender-Specific Oxidative Stress Parameters",
volume = "32",
number = "1",
pages = "14-19",
doi = "10.1055/s-0030-1267930"
}

Dopsaj, V., Martinović, J., Dopsaj, M., Kotur-Stevuljević, J.,& Bogavac-Stanojević, N.. (2011). Gender-Specific Oxidative Stress Parameters. in International Journal of Sports Medicine
Georg Thieme Verlag Kg, Stuttgart., 32(1), 14-19.
https://doi.org/10.1055/s-0030-1267930

Dopsaj V, Martinović J, Dopsaj M, Kotur-Stevuljević J, Bogavac-Stanojević N. Gender-Specific Oxidative Stress Parameters. in International Journal of Sports Medicine. 2011;32(1):14-19.
doi:10.1055/s-0030-1267930 .

Dopsaj, Violeta, Martinović, J., Dopsaj, Milivoj, Kotur-Stevuljević, Jelena, Bogavac-Stanojević, Nataša, "Gender-Specific Oxidative Stress Parameters" in International Journal of Sports Medicine, 32, no. 1 (2011):14-19,
https://doi.org/10.1055/s-0030-1267930 . .

TY  - JOUR
AU  - Martinović, J.
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Dopsaj, Milivoj
AU  - Vujović, Ana
AU  - Stefanović, Aleksandra
AU  - Nesić, G.
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1523
AB  - Aim. The objectives of this study were: 1) to evaluate pro-oxidant-antioxidant balance (PAB) associations with the commonly measured parameters of oxidative stress and antioxidative defence in elite female volleyball athletes; 2) to investigate changes in the parameters of oxidative stress during a period of intense training and dietary antioxidant supplementation. Methods. Twenty-seven female volleyball players participated in this study. Blood samples were collected the day before the pre-competitive mesocycle training period began. After the first blood sample donation and during the next six weeks fourteen players (supplemented group) received a cocktail of antioxidants while thirteen of them (control group) received no dietary supplementation. The following parameters were measured: reactive oxygen metabolites (ROMs), superoxide anion (O(2)(-)), malondialdehyde (AIDA), advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), paraoxonase activity toward paraoxon (POase) and diazoxon (DZOase), superoxide dismutase (SOD), total sulphydryl group concentration (SH groups) and pro-oxidant-antioxidant balance. Results. Significant associations were observed between biomarkers of oxidative damage with PAB in multiple linear regression model in the supplemented and the control groups (82.3% vs. 83.1%) before training and in the control group (82.1%) after training. Significant associations between anti-oxidative defence parameters and PAB values were found in the supplemented group after six-weeks of training (57%). Conclusion. In the absence of antioxidant supplementation, PAB values were dependent on the association with biomarkers of oxidative damage before and after training. After a six-week training period and the applied antioxidant supplementation, PAB values were under the influence of nonenzymatic anti-oxidative defence.
PB  - Edizioni Minerva Medica, Turin
T2  - Journal of Sports Medicine and Physical Fitness
T1  - Proxidant antioxidant balance in supplemented elite female volleyball athletes during a six week training period
VL  - 51
IS  - 1
SP  - 145
EP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1523
ER  - 

@article{
author = "Martinović, J. and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Dopsaj, Milivoj and Vujović, Ana and Stefanović, Aleksandra and Nesić, G.",
year = "2011",
abstract = "Aim. The objectives of this study were: 1) to evaluate pro-oxidant-antioxidant balance (PAB) associations with the commonly measured parameters of oxidative stress and antioxidative defence in elite female volleyball athletes; 2) to investigate changes in the parameters of oxidative stress during a period of intense training and dietary antioxidant supplementation. Methods. Twenty-seven female volleyball players participated in this study. Blood samples were collected the day before the pre-competitive mesocycle training period began. After the first blood sample donation and during the next six weeks fourteen players (supplemented group) received a cocktail of antioxidants while thirteen of them (control group) received no dietary supplementation. The following parameters were measured: reactive oxygen metabolites (ROMs), superoxide anion (O(2)(-)), malondialdehyde (AIDA), advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), biological antioxidative potential (BAP), paraoxonase activity toward paraoxon (POase) and diazoxon (DZOase), superoxide dismutase (SOD), total sulphydryl group concentration (SH groups) and pro-oxidant-antioxidant balance. Results. Significant associations were observed between biomarkers of oxidative damage with PAB in multiple linear regression model in the supplemented and the control groups (82.3% vs. 83.1%) before training and in the control group (82.1%) after training. Significant associations between anti-oxidative defence parameters and PAB values were found in the supplemented group after six-weeks of training (57%). Conclusion. In the absence of antioxidant supplementation, PAB values were dependent on the association with biomarkers of oxidative damage before and after training. After a six-week training period and the applied antioxidant supplementation, PAB values were under the influence of nonenzymatic anti-oxidative defence.",
publisher = "Edizioni Minerva Medica, Turin",
journal = "Journal of Sports Medicine and Physical Fitness",
title = "Proxidant antioxidant balance in supplemented elite female volleyball athletes during a six week training period",
volume = "51",
number = "1",
pages = "145-152",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1523"
}

Martinović, J., Dopsaj, V., Kotur-Stevuljević, J., Dopsaj, M., Vujović, A., Stefanović, A.,& Nesić, G.. (2011). Proxidant antioxidant balance in supplemented elite female volleyball athletes during a six week training period. in Journal of Sports Medicine and Physical Fitness
Edizioni Minerva Medica, Turin., 51(1), 145-152.
https://hdl.handle.net/21.15107/rcub_farfar_1523

Martinović J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Vujović A, Stefanović A, Nesić G. Proxidant antioxidant balance in supplemented elite female volleyball athletes during a six week training period. in Journal of Sports Medicine and Physical Fitness. 2011;51(1):145-152.
https://hdl.handle.net/21.15107/rcub_farfar_1523 .

Martinović, J., Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Dopsaj, Milivoj, Vujović, Ana, Stefanović, Aleksandra, Nesić, G., "Proxidant antioxidant balance in supplemented elite female volleyball athletes during a six week training period" in Journal of Sports Medicine and Physical Fitness, 51, no. 1 (2011):145-152,
https://hdl.handle.net/21.15107/rcub_farfar_1523 .

TY  - JOUR
AU  - Martinović, J.
AU  - Dopsaj, Violeta
AU  - Dopsaj, Milivoj
AU  - Kotur-Stevuljević, Jelena
AU  - Vujović, Ana
AU  - Stefanović, Aleksandra
AU  - Nesić, G.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1213
AB  - The aim of this study was to determine the impact of long-term training on elite female volleyball players and to determine parameters that could discriminate them according to the level of oxidative stress-associated adaptation. Fifty-four elite female volleyball players were divided into 3 groups (1: below-average training experience  lt 8.0 years, 2: average training experience between 8.0 and 10.5 years and 3: above-average training experience > 10.5 years). The measured parameters were reactive oxygen metabolites, biological anti-oxidative potential, superoxide anion, malondialdehyde, advanced oxidation protein products, lipid hydroperoxides, paraoxonase activity, superoxide-dismutase activity and sulphydryl groups. Multiple discriminant analysis of the oxidative stress status parameters between the three groups of athletes indicated a statistically significant difference (Wilks' lambda=0.458, X-2=35.898, p=0.031). The most important discriminant variables, superoxide-dismutase and superoxide anion, were the best indicators of differences between groups with different training experience. The significantly higher values were found in Group 3 compared with Group 1 in superoxide-dismutase activity (141 +/- 32 vs. 86 +/- 46; p=0.002), sulphydryl groups (p=0.031), and reactive oxygen metabolites (p = 0.042). The significantly lower superoxide anion was found between Group 3 and Group 1 (377 +/- 187 vs. 1183 +/- 905; p=0.001). Oxidative stress status parameters adequately discriminated 68.5% of athletes with different training experience.
PB  - Georg Thieme Verlag Kg, Stuttgart
T2  - International Journal of Sports Medicine
T1  - Long-term Effects of Oxidative Stress in Volleyball Players
VL  - 30
IS  - 12
SP  - 851
EP  - 856
DO  - 10.1055/s-0029-1238289
ER  - 

@article{
author = "Martinović, J. and Dopsaj, Violeta and Dopsaj, Milivoj and Kotur-Stevuljević, Jelena and Vujović, Ana and Stefanović, Aleksandra and Nesić, G.",
year = "2009",
abstract = "The aim of this study was to determine the impact of long-term training on elite female volleyball players and to determine parameters that could discriminate them according to the level of oxidative stress-associated adaptation. Fifty-four elite female volleyball players were divided into 3 groups (1: below-average training experience  lt 8.0 years, 2: average training experience between 8.0 and 10.5 years and 3: above-average training experience > 10.5 years). The measured parameters were reactive oxygen metabolites, biological anti-oxidative potential, superoxide anion, malondialdehyde, advanced oxidation protein products, lipid hydroperoxides, paraoxonase activity, superoxide-dismutase activity and sulphydryl groups. Multiple discriminant analysis of the oxidative stress status parameters between the three groups of athletes indicated a statistically significant difference (Wilks' lambda=0.458, X-2=35.898, p=0.031). The most important discriminant variables, superoxide-dismutase and superoxide anion, were the best indicators of differences between groups with different training experience. The significantly higher values were found in Group 3 compared with Group 1 in superoxide-dismutase activity (141 +/- 32 vs. 86 +/- 46; p=0.002), sulphydryl groups (p=0.031), and reactive oxygen metabolites (p = 0.042). The significantly lower superoxide anion was found between Group 3 and Group 1 (377 +/- 187 vs. 1183 +/- 905; p=0.001). Oxidative stress status parameters adequately discriminated 68.5% of athletes with different training experience.",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "International Journal of Sports Medicine",
title = "Long-term Effects of Oxidative Stress in Volleyball Players",
volume = "30",
number = "12",
pages = "851-856",
doi = "10.1055/s-0029-1238289"
}

Martinović, J., Dopsaj, V., Dopsaj, M., Kotur-Stevuljević, J., Vujović, A., Stefanović, A.,& Nesić, G.. (2009). Long-term Effects of Oxidative Stress in Volleyball Players. in International Journal of Sports Medicine
Georg Thieme Verlag Kg, Stuttgart., 30(12), 851-856.
https://doi.org/10.1055/s-0029-1238289

Martinović J, Dopsaj V, Dopsaj M, Kotur-Stevuljević J, Vujović A, Stefanović A, Nesić G. Long-term Effects of Oxidative Stress in Volleyball Players. in International Journal of Sports Medicine. 2009;30(12):851-856.
doi:10.1055/s-0029-1238289 .

Martinović, J., Dopsaj, Violeta, Dopsaj, Milivoj, Kotur-Stevuljević, Jelena, Vujović, Ana, Stefanović, Aleksandra, Nesić, G., "Long-term Effects of Oxidative Stress in Volleyball Players" in International Journal of Sports Medicine, 30, no. 12 (2009):851-856,
https://doi.org/10.1055/s-0029-1238289 . .