TY  - JOUR
AU  - Vekić, Jelena
AU  - Kotur-Stevuljević, Jelena
AU  - Jelić-Ivanović, Zorana
AU  - Spasić, S.
AU  - Spasojević-Kalimanovska, Vesna
AU  - Topić, Aleksandra
AU  - Zeljković, Aleksandra
AU  - Stefanović, A.
AU  - Zunić, G.
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/979
AB  - Background Alterations in plasma lipoprotein subclass distributions affect atherosclerosis risk. Smaller, denser low-density lipoprotein (LDL) particles (sdLDL) are more susceptible to oxidation. In contrast, most of the protective effects of high-density lipoproteins (HDL) are attributable to larger particles. This study investigates the connection between LDL and HDL particle heterogeneity and oxidative stress, antioxidative defence (AOD) and paraoxonase (PON1) status in a healthy middle-aged Serbian population. Materials and methods LDL and HDL particle sizes and subclass distributions were measured by gradient gel electrophoresis in 104 men and 103 women, aged 53 +/- 9.4 years. PON1 activities and PON1(Q192R) phenotypes were determined with paraoxon and diazoxon as substrates. The oxidative stress/AOD status was estimated by measuring malondialdehyde (MDA) and superoxide-anion (O-2(.-)) levels and superoxide-dismutase (SOD) activity. Results Subjects with sdLDL had significantly higher MDA (P  lt  0.001) and O-2(.-) (P  lt  0.05) levels and greater diazoxonase (DZOase) activity (P  lt  0.05) compared to subjects with larger LDL particles. A high MDA concentration was a significant predictor of the sdLDL phenotype (P  lt  0.005). Increased levels of O-2(.-) and MDA were associated with smaller HDL3 subclass abundance. Reduced HDL particle size was associated with lower DZOase activity (P  lt  0.01). Conclusions Even in the absence of symptoms of atherosclerosis, sdLDL particles are associated with increased oxidative stress, which may stimulate a compensatory rise in PON1 DZOase activity. Elevated oxidative stress may significantly affect HDL subclass distribution, resulting in the accumulation of smaller, denser HDL particles with diminished antioxidative capacity.
PB  - Wiley, Hoboken
T2  - European Journal of Clinical Investigation
T1  - Association of oxidative stress and PON1 with LDL and HDL particle size in middle-aged subjects
VL  - 37
IS  - 9
SP  - 715
EP  - 723
DO  - 10.1111/j.1365-2362.2007.01849.x
ER  - 

@article{
author = "Vekić, Jelena and Kotur-Stevuljević, Jelena and Jelić-Ivanović, Zorana and Spasić, S. and Spasojević-Kalimanovska, Vesna and Topić, Aleksandra and Zeljković, Aleksandra and Stefanović, A. and Zunić, G.",
year = "2007",
abstract = "Background Alterations in plasma lipoprotein subclass distributions affect atherosclerosis risk. Smaller, denser low-density lipoprotein (LDL) particles (sdLDL) are more susceptible to oxidation. In contrast, most of the protective effects of high-density lipoproteins (HDL) are attributable to larger particles. This study investigates the connection between LDL and HDL particle heterogeneity and oxidative stress, antioxidative defence (AOD) and paraoxonase (PON1) status in a healthy middle-aged Serbian population. Materials and methods LDL and HDL particle sizes and subclass distributions were measured by gradient gel electrophoresis in 104 men and 103 women, aged 53 +/- 9.4 years. PON1 activities and PON1(Q192R) phenotypes were determined with paraoxon and diazoxon as substrates. The oxidative stress/AOD status was estimated by measuring malondialdehyde (MDA) and superoxide-anion (O-2(.-)) levels and superoxide-dismutase (SOD) activity. Results Subjects with sdLDL had significantly higher MDA (P  lt  0.001) and O-2(.-) (P  lt  0.05) levels and greater diazoxonase (DZOase) activity (P  lt  0.05) compared to subjects with larger LDL particles. A high MDA concentration was a significant predictor of the sdLDL phenotype (P  lt  0.005). Increased levels of O-2(.-) and MDA were associated with smaller HDL3 subclass abundance. Reduced HDL particle size was associated with lower DZOase activity (P  lt  0.01). Conclusions Even in the absence of symptoms of atherosclerosis, sdLDL particles are associated with increased oxidative stress, which may stimulate a compensatory rise in PON1 DZOase activity. Elevated oxidative stress may significantly affect HDL subclass distribution, resulting in the accumulation of smaller, denser HDL particles with diminished antioxidative capacity.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Clinical Investigation",
title = "Association of oxidative stress and PON1 with LDL and HDL particle size in middle-aged subjects",
volume = "37",
number = "9",
pages = "715-723",
doi = "10.1111/j.1365-2362.2007.01849.x"
}

Vekić, J., Kotur-Stevuljević, J., Jelić-Ivanović, Z., Spasić, S., Spasojević-Kalimanovska, V., Topić, A., Zeljković, A., Stefanović, A.,& Zunić, G.. (2007). Association of oxidative stress and PON1 with LDL and HDL particle size in middle-aged subjects. in European Journal of Clinical Investigation
Wiley, Hoboken., 37(9), 715-723.
https://doi.org/10.1111/j.1365-2362.2007.01849.x

Vekić J, Kotur-Stevuljević J, Jelić-Ivanović Z, Spasić S, Spasojević-Kalimanovska V, Topić A, Zeljković A, Stefanović A, Zunić G. Association of oxidative stress and PON1 with LDL and HDL particle size in middle-aged subjects. in European Journal of Clinical Investigation. 2007;37(9):715-723.
doi:10.1111/j.1365-2362.2007.01849.x .

Vekić, Jelena, Kotur-Stevuljević, Jelena, Jelić-Ivanović, Zorana, Spasić, S., Spasojević-Kalimanovska, Vesna, Topić, Aleksandra, Zeljković, Aleksandra, Stefanović, A., Zunić, G., "Association of oxidative stress and PON1 with LDL and HDL particle size in middle-aged subjects" in European Journal of Clinical Investigation, 37, no. 9 (2007):715-723,
https://doi.org/10.1111/j.1365-2362.2007.01849.x . .

TY  - JOUR
AU  - Zunić, G
AU  - Jelić-Ivanović, Zorana
AU  - Colić, M
AU  - Spasić, S
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/328
AB  - This report describes a rapid, single-run procedure, based on the optimization of capillary electrophoresis (CE) and indirect absorbance detection capabilities, which was developed for the separation and quantification of 30 underivatized physiological amino acids and peptides, usually present in biological fluids. p-Aminosalicylic acid buffered with sodium carbonate at pH 10.2 +/- 0.1 was used as the running electrolyte. Electrophoresis, carried out in a capillary (87 cmx75 mum) at 15 kV potential (normal polarity), separated the examined compounds within 30 min. Limits of detection ranged from 1.93 to 20.08 mumol/l (median 6.71 mumol/l). The method was linear within the 50-200 mumol/l concentration range (r ranged from 0.684 to 0.989, median r=0.934). Within run migration times precision was good (median C.V.=0.7%). Less favorable within run peak area precision (median C.V.=6.6%) was obtained. The analytical procedure presented was successfully tested for separation and quantification of amino acids in physiological fluids, such as plasma or supernatant of macrophage cultures. Sample preparations require only a protein precipitation and dilution step.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids
VL  - 772
IS  - 1
SP  - 19
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_farfar_328
ER  - 

@article{
author = "Zunić, G and Jelić-Ivanović, Zorana and Colić, M and Spasić, S",
year = "2002",
abstract = "This report describes a rapid, single-run procedure, based on the optimization of capillary electrophoresis (CE) and indirect absorbance detection capabilities, which was developed for the separation and quantification of 30 underivatized physiological amino acids and peptides, usually present in biological fluids. p-Aminosalicylic acid buffered with sodium carbonate at pH 10.2 +/- 0.1 was used as the running electrolyte. Electrophoresis, carried out in a capillary (87 cmx75 mum) at 15 kV potential (normal polarity), separated the examined compounds within 30 min. Limits of detection ranged from 1.93 to 20.08 mumol/l (median 6.71 mumol/l). The method was linear within the 50-200 mumol/l concentration range (r ranged from 0.684 to 0.989, median r=0.934). Within run migration times precision was good (median C.V.=0.7%). Less favorable within run peak area precision (median C.V.=6.6%) was obtained. The analytical procedure presented was successfully tested for separation and quantification of amino acids in physiological fluids, such as plasma or supernatant of macrophage cultures. Sample preparations require only a protein precipitation and dilution step.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids",
volume = "772",
number = "1",
pages = "19-33",
url = "https://hdl.handle.net/21.15107/rcub_farfar_328"
}

Zunić, G., Jelić-Ivanović, Z., Colić, M.,& Spasić, S.. (2002). Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 772(1), 19-33.
https://hdl.handle.net/21.15107/rcub_farfar_328

Zunić G, Jelić-Ivanović Z, Colić M, Spasić S. Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2002;772(1):19-33.
https://hdl.handle.net/21.15107/rcub_farfar_328 .

Zunić, G, Jelić-Ivanović, Zorana, Colić, M, Spasić, S, "Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 772, no. 1 (2002):19-33,
https://hdl.handle.net/21.15107/rcub_farfar_328 .

TY  - JOUR
AU  - Zunić, G
AU  - Spasić, S
AU  - Jelić-Ivanović, Zorana
PY  - 1999
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/221
AB  - Nitrite and nitrate levels in physiological fluids are commonly used as an index of nitric oxide production. We developed simple and rapid method for the determination of these anions by capillary zone electrophoresis employing berate buffer (pH 10, 100 mmol/l) as running electrolyte. The anions were analyzed in plasma and cerebrospinal fluid (CSF) without deproteinization of the samples. Electrophoresis was carried out in a capillary (36.5 cm X 75 mu m) at a potential of 15 kV, with on-column UV detection at 214 nm. Mean retention times for nitrite and nitrates were 4.631 and 5.152 min, respectively. The method was linear (r = 0.999) within a 1-500 mu mol/l concentration range. Physiological levels of nitrate in plasma (40.2 mu mol/l) and CSF (15.3 mu mol/l) could be determined with good precision (coefficients of variation  lt 6%) and accuracy (recoveries of added nitrate to plasma and CSF were 97.4 and 104.5%, respectively). Measurements of the physiological levels of nitrite in plasma (6.1 mu mol/l) and CSF (0.9 mu mol/l) were less precise and accurate.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B
T1  - Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis
VL  - 727
IS  - 1-2
SP  - 73
EP  - 79
DO  - 10.1016/S0378-4347(99)00054-7
ER  - 

@article{
author = "Zunić, G and Spasić, S and Jelić-Ivanović, Zorana",
year = "1999",
abstract = "Nitrite and nitrate levels in physiological fluids are commonly used as an index of nitric oxide production. We developed simple and rapid method for the determination of these anions by capillary zone electrophoresis employing berate buffer (pH 10, 100 mmol/l) as running electrolyte. The anions were analyzed in plasma and cerebrospinal fluid (CSF) without deproteinization of the samples. Electrophoresis was carried out in a capillary (36.5 cm X 75 mu m) at a potential of 15 kV, with on-column UV detection at 214 nm. Mean retention times for nitrite and nitrates were 4.631 and 5.152 min, respectively. The method was linear (r = 0.999) within a 1-500 mu mol/l concentration range. Physiological levels of nitrate in plasma (40.2 mu mol/l) and CSF (15.3 mu mol/l) could be determined with good precision (coefficients of variation  lt 6%) and accuracy (recoveries of added nitrate to plasma and CSF were 97.4 and 104.5%, respectively). Measurements of the physiological levels of nitrite in plasma (6.1 mu mol/l) and CSF (0.9 mu mol/l) were less precise and accurate.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B",
title = "Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis",
volume = "727",
number = "1-2",
pages = "73-79",
doi = "10.1016/S0378-4347(99)00054-7"
}

Zunić, G., Spasić, S.,& Jelić-Ivanović, Z.. (1999). Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis. in Journal of Chromatography B
Elsevier Science BV, Amsterdam., 727(1-2), 73-79.
https://doi.org/10.1016/S0378-4347(99)00054-7

Zunić G, Spasić S, Jelić-Ivanović Z. Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis. in Journal of Chromatography B. 1999;727(1-2):73-79.
doi:10.1016/S0378-4347(99)00054-7 .

Zunić, G, Spasić, S, Jelić-Ivanović, Zorana, "Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis" in Journal of Chromatography B, 727, no. 1-2 (1999):73-79,
https://doi.org/10.1016/S0378-4347(99)00054-7 . .