TY  - JOUR
AU  - Vujović, Ana
AU  - Kotur-Stevuljević, Jelena
AU  - Spasić, Slavica
AU  - Bujisić, Nada
AU  - Martinović, Jelena
AU  - Vujović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Aleksandra
AU  - Pajić, Dragoljub
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1374
AB  - Background: Friedewald's formula for the estimation of LDL-C concentration is the most often used formula in clinical practice. A recent formula by Anandaraja and colleagues for LDL-C estimation still needs to be evaluated before it is extensively applied in diagnosis. In the present study we validated existing formulas and derived a more accurate formula to determine LDL-C in a Serbian population. Methods: Our study included 2053 patients with TG  lt = 4.52 mmol/L. In an initial group of 1010 patients, Friedewald's and Anandaraja's formulas were compared to a direct homogenous method for LDL-C determination. The obtained results allowed us to modify Friedewald's formula and apply it in a second group of patients. Results: The mean LDL-C concentrations were 3.9 +/- 1.09 mmol/L, 3.63 +/- 1.06 mmol/L and 3.72 +/- 1.04 mmol/L measured by a direct homogenous assay (D-LDL-C), calculated by Friedewald's formula (F-LDL-C) and calculated by Anandaraja's formula (A-LDL-C), respectively in the 1010 patients. The Student's paired t-test showed that D-LDL-C values were significantly higher than F-LDL-C and A-LDL-C values (p  lt  0.001). The Passing-Bablok regression analysis indicated good correlation between calculated and measured LDL-Cs (r > 0.89). Using lipoprotein values from the initial group we modified Friedewald's formula by replacing the term 2.2 with 3. The new modified formula for LDL-C estimation (S-LDL-C) showed no statistically significant difference compared to D-LDL-C. The absolute bias between these two methods was -0.06 +/- 0.37 mmol/L with a high correlation coefficient (r = 0.96). Conclusions: Our modified formula for LDL-C estimation appears to be more accurate than both Friedewald's and Anandaraja's formulas when applied to a Serbian population.
PB  - BMC, LONDON
T2  - Lipids in Health and Disease
T1  - Evaluation of different formulas for LDL-C calculation
VL  - 9
DO  - 10.1186/1476-511X-9-27
ER  - 

@article{
author = "Vujović, Ana and Kotur-Stevuljević, Jelena and Spasić, Slavica and Bujisić, Nada and Martinović, Jelena and Vujović, Milica and Spasojević-Kalimanovska, Vesna and Zeljković, Aleksandra and Pajić, Dragoljub",
year = "2010",
abstract = "Background: Friedewald's formula for the estimation of LDL-C concentration is the most often used formula in clinical practice. A recent formula by Anandaraja and colleagues for LDL-C estimation still needs to be evaluated before it is extensively applied in diagnosis. In the present study we validated existing formulas and derived a more accurate formula to determine LDL-C in a Serbian population. Methods: Our study included 2053 patients with TG  lt = 4.52 mmol/L. In an initial group of 1010 patients, Friedewald's and Anandaraja's formulas were compared to a direct homogenous method for LDL-C determination. The obtained results allowed us to modify Friedewald's formula and apply it in a second group of patients. Results: The mean LDL-C concentrations were 3.9 +/- 1.09 mmol/L, 3.63 +/- 1.06 mmol/L and 3.72 +/- 1.04 mmol/L measured by a direct homogenous assay (D-LDL-C), calculated by Friedewald's formula (F-LDL-C) and calculated by Anandaraja's formula (A-LDL-C), respectively in the 1010 patients. The Student's paired t-test showed that D-LDL-C values were significantly higher than F-LDL-C and A-LDL-C values (p  lt  0.001). The Passing-Bablok regression analysis indicated good correlation between calculated and measured LDL-Cs (r > 0.89). Using lipoprotein values from the initial group we modified Friedewald's formula by replacing the term 2.2 with 3. The new modified formula for LDL-C estimation (S-LDL-C) showed no statistically significant difference compared to D-LDL-C. The absolute bias between these two methods was -0.06 +/- 0.37 mmol/L with a high correlation coefficient (r = 0.96). Conclusions: Our modified formula for LDL-C estimation appears to be more accurate than both Friedewald's and Anandaraja's formulas when applied to a Serbian population.",
publisher = "BMC, LONDON",
journal = "Lipids in Health and Disease",
title = "Evaluation of different formulas for LDL-C calculation",
volume = "9",
doi = "10.1186/1476-511X-9-27"
}

Vujović, A., Kotur-Stevuljević, J., Spasić, S., Bujisić, N., Martinović, J., Vujović, M., Spasojević-Kalimanovska, V., Zeljković, A.,& Pajić, D.. (2010). Evaluation of different formulas for LDL-C calculation. in Lipids in Health and Disease
BMC, LONDON., 9.
https://doi.org/10.1186/1476-511X-9-27

Vujović A, Kotur-Stevuljević J, Spasić S, Bujisić N, Martinović J, Vujović M, Spasojević-Kalimanovska V, Zeljković A, Pajić D. Evaluation of different formulas for LDL-C calculation. in Lipids in Health and Disease. 2010;9.
doi:10.1186/1476-511X-9-27 .

Vujović, Ana, Kotur-Stevuljević, Jelena, Spasić, Slavica, Bujisić, Nada, Martinović, Jelena, Vujović, Milica, Spasojević-Kalimanovska, Vesna, Zeljković, Aleksandra, Pajić, Dragoljub, "Evaluation of different formulas for LDL-C calculation" in Lipids in Health and Disease, 9 (2010),
https://doi.org/10.1186/1476-511X-9-27 . .

TY  - JOUR
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Spasić, Slavica
AU  - Vekić, Jelena
AU  - Bujisić, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1226
AB  - Objectives: Oxidative stress and paraoxonase activity play a significant role in the pathogenesis of cardiovascular disease (CVD). The Prospective Cardiovascular Munster (PROCAM) study evaluated the prevalence of CVD risk factors and postulated the prediction of future CVD events. We therefore investigated the association between plasma markers of oxidative stress and paraoxonase status With PROCAM risk score. Design and methods: Oxidative stress status parameters [lipid peroxidation measured as thiobarbituric acid-reacting substances (TBARS), superoxide anion (O-2 center dot(-)), superoxide dismutase (SOD) activity, total sulphydryl group content] and paraoxonase (PONI) status were assessed in 211 participants. The predicted 10-year risk was calculated according to the PROCAM algorithm. Results: As expected subjects with high PROCAM risk score (high CVD risk) had significantly higher concentrations of oxidative stress parameters (TBARS and O-2 center dot(-) P lt 0-001 and P lt 0.05, respectively). The PONI192 phenotype distribution among CVD risk groups was not significantly different. Logistic regression analyses revealed significant associations of all the examined oxidative stress status parameters with calculated CVD risk score. The potential of the parameters for CVD risk prediction was tested via multivariate analysis. Only the O-2 center dot(-) level retained a strong association with high CVD risk. Conclusions: Our study demonstrated that high PROCAM risk score is associated with increased oxidative stress, indicating for the first time that elevated O-2 center dot(-) is independently associated with high CVD, risk.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Association of oxidative stress and paraoxonase status with PROCAM risk score
VL  - 42
IS  - 7-8
SP  - 617
EP  - 623
DO  - 10.1016/j.clinbiochem.2009.01.008
ER  - 

@article{
author = "Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Spasić, Slavica and Vekić, Jelena and Bujisić, Nada",
year = "2009",
abstract = "Objectives: Oxidative stress and paraoxonase activity play a significant role in the pathogenesis of cardiovascular disease (CVD). The Prospective Cardiovascular Munster (PROCAM) study evaluated the prevalence of CVD risk factors and postulated the prediction of future CVD events. We therefore investigated the association between plasma markers of oxidative stress and paraoxonase status With PROCAM risk score. Design and methods: Oxidative stress status parameters [lipid peroxidation measured as thiobarbituric acid-reacting substances (TBARS), superoxide anion (O-2 center dot(-)), superoxide dismutase (SOD) activity, total sulphydryl group content] and paraoxonase (PONI) status were assessed in 211 participants. The predicted 10-year risk was calculated according to the PROCAM algorithm. Results: As expected subjects with high PROCAM risk score (high CVD risk) had significantly higher concentrations of oxidative stress parameters (TBARS and O-2 center dot(-) P lt 0-001 and P lt 0.05, respectively). The PONI192 phenotype distribution among CVD risk groups was not significantly different. Logistic regression analyses revealed significant associations of all the examined oxidative stress status parameters with calculated CVD risk score. The potential of the parameters for CVD risk prediction was tested via multivariate analysis. Only the O-2 center dot(-) level retained a strong association with high CVD risk. Conclusions: Our study demonstrated that high PROCAM risk score is associated with increased oxidative stress, indicating for the first time that elevated O-2 center dot(-) is independently associated with high CVD, risk.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Association of oxidative stress and paraoxonase status with PROCAM risk score",
volume = "42",
number = "7-8",
pages = "617-623",
doi = "10.1016/j.clinbiochem.2009.01.008"
}

Stefanović, A., Kotur-Stevuljević, J., Spasić, S., Vekić, J.,& Bujisić, N.. (2009). Association of oxidative stress and paraoxonase status with PROCAM risk score. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 42(7-8), 617-623.
https://doi.org/10.1016/j.clinbiochem.2009.01.008

Stefanović A, Kotur-Stevuljević J, Spasić S, Vekić J, Bujisić N. Association of oxidative stress and paraoxonase status with PROCAM risk score. in Clinical Biochemistry. 2009;42(7-8):617-623.
doi:10.1016/j.clinbiochem.2009.01.008 .

Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Spasić, Slavica, Vekić, Jelena, Bujisić, Nada, "Association of oxidative stress and paraoxonase status with PROCAM risk score" in Clinical Biochemistry, 42, no. 7-8 (2009):617-623,
https://doi.org/10.1016/j.clinbiochem.2009.01.008 . .

TY  - JOUR
AU  - Jelić-Ivanović, Zorana
AU  - Bujisić, Nada
AU  - Spasić, Slavica
AU  - Bogavac-Stanojević, Nataša
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1210
AB  - Objectives: Decreased concentrations of circulating soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) were recently reported to be associated with atherosclerosis, but there are still no data concerning its predictive performance. Design and methods: The current cross-sectional study investigates the potential of the novel atherosclerotic biomarker for the prediction of coronary artery disease (CAD). Serum sTWEAK was measured by ELISA in 76 CAD patients and 82 CAD-free subjects. Results: Serum sTWEAK concentrations were significantly lower in the patients (534.5 +/- 110.9 mu g/L) than in the controls (688.1 +/- 150.0 mu g/L, p  lt  0.001), even after adjusting for different confounders (p  lt  0.001). The areas under ROC curves (AUC)s calculated for logistic regression models that included different known risk factors were significantly increased when sTWEAK was added to the corresponding model (p = 0.011-0.035). Conclusions: The measurement of serum sTWEAK concentrations improves the prediction of CAD based on existing biomarkers.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Circulating sTWEAK improves the prediction of coronary artery disease
VL  - 42
IS  - 13-14
SP  - 1381
EP  - 1386
DO  - 10.1016/j.clinbiochem.2009.06.001
ER  - 

@article{
author = "Jelić-Ivanović, Zorana and Bujisić, Nada and Spasić, Slavica and Bogavac-Stanojević, Nataša and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena",
year = "2009",
abstract = "Objectives: Decreased concentrations of circulating soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) were recently reported to be associated with atherosclerosis, but there are still no data concerning its predictive performance. Design and methods: The current cross-sectional study investigates the potential of the novel atherosclerotic biomarker for the prediction of coronary artery disease (CAD). Serum sTWEAK was measured by ELISA in 76 CAD patients and 82 CAD-free subjects. Results: Serum sTWEAK concentrations were significantly lower in the patients (534.5 +/- 110.9 mu g/L) than in the controls (688.1 +/- 150.0 mu g/L, p  lt  0.001), even after adjusting for different confounders (p  lt  0.001). The areas under ROC curves (AUC)s calculated for logistic regression models that included different known risk factors were significantly increased when sTWEAK was added to the corresponding model (p = 0.011-0.035). Conclusions: The measurement of serum sTWEAK concentrations improves the prediction of CAD based on existing biomarkers.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Circulating sTWEAK improves the prediction of coronary artery disease",
volume = "42",
number = "13-14",
pages = "1381-1386",
doi = "10.1016/j.clinbiochem.2009.06.001"
}

Jelić-Ivanović, Z., Bujisić, N., Spasić, S., Bogavac-Stanojević, N., Spasojević-Kalimanovska, V.,& Kotur-Stevuljević, J.. (2009). Circulating sTWEAK improves the prediction of coronary artery disease. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 42(13-14), 1381-1386.
https://doi.org/10.1016/j.clinbiochem.2009.06.001

Jelić-Ivanović Z, Bujisić N, Spasić S, Bogavac-Stanojević N, Spasojević-Kalimanovska V, Kotur-Stevuljević J. Circulating sTWEAK improves the prediction of coronary artery disease. in Clinical Biochemistry. 2009;42(13-14):1381-1386.
doi:10.1016/j.clinbiochem.2009.06.001 .

Jelić-Ivanović, Zorana, Bujisić, Nada, Spasić, Slavica, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, "Circulating sTWEAK improves the prediction of coronary artery disease" in Clinical Biochemistry, 42, no. 13-14 (2009):1381-1386,
https://doi.org/10.1016/j.clinbiochem.2009.06.001 . .

TY  - JOUR
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Spasić, Slavica
AU  - Bogavac-Stanojević, Nataša
AU  - Bujisić, Nada
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1043
AB  - The aim of this study was to determinate both the oxidative stress/anti-oxidative defense status and the concentration of leptin in obese, overweight and normal weight type 2 diabetes mellitus patients to seek possible association between oxidative stress and hyperleptinemia. Oxidative stress status parameters [thiobarbituric acid-reacting substances (TBARS), superoxide anion (O-2(center dot-)), superoxide dismutase (SOD) activity and total sulphydryl groups] and the concentration of leptin were measured in 312 subjects (178 patients and in 134 control subjects). Obese patients had a significantly higher concentration of leptin compared to obese subjects in the control population (P  lt  0.001). They also had significantly higher plasma concentrations of TBARS, O-2(center dot-) and SOD activity in combination with a lower sulphydryl group concentration when compared to control subjects. Obese patients had significantly higher concentrations of both TBARS and O-2(center dot-) and increased SOD activity compared to normal weight patients. The odds ratio for the degree of association between oxidative stress status parameters and hyperleptinemia was strongest for TBARS [odds ratio 2.66, 95% CI (1.02-6.94), P = 0.045]. The observed positive correlation between TBARS and leptin (p = 0.29, P  lt  0.01) in obese patients suggests that increased oxidative stress and hyperleptinemia, both consequences of obesity, may play a role in type 2 diabetes mellitus development.
PB  - Elsevier Ireland Ltd, Clare
T2  - Diabetes Research and Clinical Practice
T1  - The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients
VL  - 79
IS  - 1
SP  - 156
EP  - 163
DO  - 10.1016/j.diabres.2007.07.019
ER  - 

@article{
author = "Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Spasić, Slavica and Bogavac-Stanojević, Nataša and Bujisić, Nada",
year = "2008",
abstract = "The aim of this study was to determinate both the oxidative stress/anti-oxidative defense status and the concentration of leptin in obese, overweight and normal weight type 2 diabetes mellitus patients to seek possible association between oxidative stress and hyperleptinemia. Oxidative stress status parameters [thiobarbituric acid-reacting substances (TBARS), superoxide anion (O-2(center dot-)), superoxide dismutase (SOD) activity and total sulphydryl groups] and the concentration of leptin were measured in 312 subjects (178 patients and in 134 control subjects). Obese patients had a significantly higher concentration of leptin compared to obese subjects in the control population (P  lt  0.001). They also had significantly higher plasma concentrations of TBARS, O-2(center dot-) and SOD activity in combination with a lower sulphydryl group concentration when compared to control subjects. Obese patients had significantly higher concentrations of both TBARS and O-2(center dot-) and increased SOD activity compared to normal weight patients. The odds ratio for the degree of association between oxidative stress status parameters and hyperleptinemia was strongest for TBARS [odds ratio 2.66, 95% CI (1.02-6.94), P = 0.045]. The observed positive correlation between TBARS and leptin (p = 0.29, P  lt  0.01) in obese patients suggests that increased oxidative stress and hyperleptinemia, both consequences of obesity, may play a role in type 2 diabetes mellitus development.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Diabetes Research and Clinical Practice",
title = "The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients",
volume = "79",
number = "1",
pages = "156-163",
doi = "10.1016/j.diabres.2007.07.019"
}

Stefanović, A., Kotur-Stevuljević, J., Spasić, S., Bogavac-Stanojević, N.,& Bujisić, N.. (2008). The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients. in Diabetes Research and Clinical Practice
Elsevier Ireland Ltd, Clare., 79(1), 156-163.
https://doi.org/10.1016/j.diabres.2007.07.019

Stefanović A, Kotur-Stevuljević J, Spasić S, Bogavac-Stanojević N, Bujisić N. The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients. in Diabetes Research and Clinical Practice. 2008;79(1):156-163.
doi:10.1016/j.diabres.2007.07.019 .

Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Spasić, Slavica, Bogavac-Stanojević, Nataša, Bujisić, Nada, "The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients" in Diabetes Research and Clinical Practice, 79, no. 1 (2008):156-163,
https://doi.org/10.1016/j.diabres.2007.07.019 . .