TY  - JOUR
AU  - Konstantinović, Neda
AU  - Cirković, Ivana
AU  - Dukić, Slobodanka
AU  - Marić, Vesna
AU  - Božić, Dragana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2786
AB  - Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Biofilm formation of achromobacter xylosoxidans on contact lens
VL  - 64
IS  - 3
SP  - 293
EP  - 300
DO  - 10.1556/030.64.2017.005
ER  - 

@article{
author = "Konstantinović, Neda and Cirković, Ivana and Dukić, Slobodanka and Marić, Vesna and Božić, Dragana",
year = "2017",
abstract = "Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Biofilm formation of achromobacter xylosoxidans on contact lens",
volume = "64",
number = "3",
pages = "293-300",
doi = "10.1556/030.64.2017.005"
}

Konstantinović, N., Cirković, I., Dukić, S., Marić, V.,& Božić, D.. (2017). Biofilm formation of achromobacter xylosoxidans on contact lens. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 64(3), 293-300.
https://doi.org/10.1556/030.64.2017.005

Konstantinović N, Cirković I, Dukić S, Marić V, Božić D. Biofilm formation of achromobacter xylosoxidans on contact lens. in Acta Microbiologica et Immunologica Hungarica. 2017;64(3):293-300.
doi:10.1556/030.64.2017.005 .

Konstantinović, Neda, Cirković, Ivana, Dukić, Slobodanka, Marić, Vesna, Božić, Dragana, "Biofilm formation of achromobacter xylosoxidans on contact lens" in Acta Microbiologica et Immunologica Hungarica, 64, no. 3 (2017):293-300,
https://doi.org/10.1556/030.64.2017.005 . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Knežević, Miroslav
AU  - Božić, Dragana
AU  - Rasić, Dejan
AU  - Larsen, Anders Rhod
AU  - Dukić, Slobodanka
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2414
AB  - The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.
PB  - Springer, New York
T2  - Graefes Archive for Clinical and Experimental Ophthalmology
T1  - Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage
VL  - 253
IS  - 1
SP  - 77
EP  - 82
DO  - 10.1007/s00417-014-2786-0
ER  - 

@article{
author = "Cirković, Ivana and Knežević, Miroslav and Božić, Dragana and Rasić, Dejan and Larsen, Anders Rhod and Dukić, Slobodanka",
year = "2015",
abstract = "The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.",
publisher = "Springer, New York",
journal = "Graefes Archive for Clinical and Experimental Ophthalmology",
title = "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage",
volume = "253",
number = "1",
pages = "77-82",
doi = "10.1007/s00417-014-2786-0"
}

Cirković, I., Knežević, M., Božić, D., Rasić, D., Larsen, A. R.,& Dukić, S.. (2015). Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology
Springer, New York., 253(1), 77-82.
https://doi.org/10.1007/s00417-014-2786-0

Cirković I, Knežević M, Božić D, Rasić D, Larsen AR, Dukić S. Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology. 2015;253(1):77-82.
doi:10.1007/s00417-014-2786-0 .

Cirković, Ivana, Knežević, Miroslav, Božić, Dragana, Rasić, Dejan, Larsen, Anders Rhod, Dukić, Slobodanka, "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage" in Graefes Archive for Clinical and Experimental Ophthalmology, 253, no. 1 (2015):77-82,
https://doi.org/10.1007/s00417-014-2786-0 . .