TY  - JOUR
AU  - Vukelić, Dragana
AU  - Buha-Đorđević, Aleksandra
AU  - Anđelković, Milena
AU  - Repić, Aleksandra
AU  - Baralić, Katarina
AU  - Ćurčić, Marijana
AU  - Đukić-Ćosić, Danijela
AU  - Boričić, Novica
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4385
AB  - The objectives of the study were to simulate low-level Pb exposure scenario in an animal model and to examine reproductive adverse effects. Based on obtained data, we have performed Benchmark dose (BMD)-response modelling. Male Wistar rats were randomized in seven groups (n = 6): one control and six treated with: 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg body weight, daily for 28 days by oral gavage. The rats were sacrificed and the blood and testes were used for further analysis of testosterone levels in serum, testicular essential metal levels and histological analysis. The Pb treatment led to a dose-dependent decrease of serum testosterone levels with a negative trend (BMDI 0.17–6.13 mg Pb/kg). Increase of Zn (dose-dependent, BMDI 0.004–19.7 mg Pb/kg) and Cu and a decrease of Mn testicular levels were also detected with unscathed histology of the testes. The presented results might be used in further evaluation of the point of departure in human health risk assessment for Pb.
PB  - Elsevier Ireland Ltd
T2  - Toxicology Letters
T1  - Derivation of benchmark doses for male reproductive toxicity in a subacute low-level Pb exposure model in rats
VL  - 375
SP  - 69
EP  - 76
DO  - 10.1016/j.toxlet.2023.01.001
ER  - 

@article{
author = "Vukelić, Dragana and Buha-Đorđević, Aleksandra and Anđelković, Milena and Repić, Aleksandra and Baralić, Katarina and Ćurčić, Marijana and Đukić-Ćosić, Danijela and Boričić, Novica and Antonijević, Biljana and Bulat, Zorica",
year = "2023",
abstract = "The objectives of the study were to simulate low-level Pb exposure scenario in an animal model and to examine reproductive adverse effects. Based on obtained data, we have performed Benchmark dose (BMD)-response modelling. Male Wistar rats were randomized in seven groups (n = 6): one control and six treated with: 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg body weight, daily for 28 days by oral gavage. The rats were sacrificed and the blood and testes were used for further analysis of testosterone levels in serum, testicular essential metal levels and histological analysis. The Pb treatment led to a dose-dependent decrease of serum testosterone levels with a negative trend (BMDI 0.17–6.13 mg Pb/kg). Increase of Zn (dose-dependent, BMDI 0.004–19.7 mg Pb/kg) and Cu and a decrease of Mn testicular levels were also detected with unscathed histology of the testes. The presented results might be used in further evaluation of the point of departure in human health risk assessment for Pb.",
publisher = "Elsevier Ireland Ltd",
journal = "Toxicology Letters",
title = "Derivation of benchmark doses for male reproductive toxicity in a subacute low-level Pb exposure model in rats",
volume = "375",
pages = "69-76",
doi = "10.1016/j.toxlet.2023.01.001"
}

Vukelić, D., Buha-Đorđević, A., Anđelković, M., Repić, A., Baralić, K., Ćurčić, M., Đukić-Ćosić, D., Boričić, N., Antonijević, B.,& Bulat, Z.. (2023). Derivation of benchmark doses for male reproductive toxicity in a subacute low-level Pb exposure model in rats. in Toxicology Letters
Elsevier Ireland Ltd., 375, 69-76.
https://doi.org/10.1016/j.toxlet.2023.01.001

Vukelić D, Buha-Đorđević A, Anđelković M, Repić A, Baralić K, Ćurčić M, Đukić-Ćosić D, Boričić N, Antonijević B, Bulat Z. Derivation of benchmark doses for male reproductive toxicity in a subacute low-level Pb exposure model in rats. in Toxicology Letters. 2023;375:69-76.
doi:10.1016/j.toxlet.2023.01.001 .

Vukelić, Dragana, Buha-Đorđević, Aleksandra, Anđelković, Milena, Repić, Aleksandra, Baralić, Katarina, Ćurčić, Marijana, Đukić-Ćosić, Danijela, Boričić, Novica, Antonijević, Biljana, Bulat, Zorica, "Derivation of benchmark doses for male reproductive toxicity in a subacute low-level Pb exposure model in rats" in Toxicology Letters, 375 (2023):69-76,
https://doi.org/10.1016/j.toxlet.2023.01.001 . .

TY  - JOUR
AU  - Javorac, Dragana
AU  - Anđelković, Milena
AU  - Repić, Aleksandra
AU  - Tatović, Simona
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Batinić, Bojan
AU  - Boričić, Novica
AU  - Đukić-Ćosić, Danijela
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4089
AB  - Lead (Pb) is a toxic metal that affects almost all human’s system and organs, with the nervous system as the most sensitive. Better understanding of the Pb neurobehavioral effects and neurotoxicity requires realistic study scenarios based on low level exposure. The aim of this study was to determine neurotoxic effects and mechanisms of Pb in six low doses and to establish dose-response relationship for these effects and related Benchmark dose (BMD). Forty-two, male albino Wistar rats were randomized into seven groups, control and Pb-exposed: 0.1, 0.5, 1, 3, 7 and 15 mg Pb/kg body weight/day (oral gavage) for 28 days. Behavioural tests (Elevated plus maze test, Spontaneous locomotor activity test and Novel object recognition test) were conducted in the last week of experiment, in the control, lower (0.5 mg Pb/kg), middle (3 mg Pb/kg) and higher (15 mg Pb/kg) dose groups. The acetylcholinesterase activity, oxidative status and essential elements levels (Cu, Zn, Mn and Fe) were measured in brain tissue along with histological analyses. External and internal dose-response analyses were performed using PROASTweb 70.1 software. The results have shown that subacute exposure to very low doses of Pb resulted in memory deficits in rats that was accompanied with acetylcholinesterase enzyme activity decrease. The observed hyperactive behaviour was accompanied by dose-dependent induction of brain oxidative stress and Zn elevation. The histological alterations in Purkinje cells were only detected in the group treated with the highest Pb dose. The lowest BMD considering entire oxidative status was calculated based on total oxidative status (4.5e-06 mg Pb/kg b.w./day). The findings reported in our study may be beneficial in further evaluating the health consequences and human health risk assessment of low-level Pb exposure.
PB  - Elsevier Ireland Ltd
T2  - Chemico-Biological Interactions
T1  - Comprehensive insight into the neurotoxic mechanisms of low dose Pb exposure in Wistar rats: Benchmark dose analysis
VL  - 360
DO  - 10.1016/j.cbi.2022.109932
ER  - 

@article{
author = "Javorac, Dragana and Anđelković, Milena and Repić, Aleksandra and Tatović, Simona and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Batinić, Bojan and Boričić, Novica and Đukić-Ćosić, Danijela and Antonijević, Biljana and Bulat, Zorica",
year = "2022",
abstract = "Lead (Pb) is a toxic metal that affects almost all human’s system and organs, with the nervous system as the most sensitive. Better understanding of the Pb neurobehavioral effects and neurotoxicity requires realistic study scenarios based on low level exposure. The aim of this study was to determine neurotoxic effects and mechanisms of Pb in six low doses and to establish dose-response relationship for these effects and related Benchmark dose (BMD). Forty-two, male albino Wistar rats were randomized into seven groups, control and Pb-exposed: 0.1, 0.5, 1, 3, 7 and 15 mg Pb/kg body weight/day (oral gavage) for 28 days. Behavioural tests (Elevated plus maze test, Spontaneous locomotor activity test and Novel object recognition test) were conducted in the last week of experiment, in the control, lower (0.5 mg Pb/kg), middle (3 mg Pb/kg) and higher (15 mg Pb/kg) dose groups. The acetylcholinesterase activity, oxidative status and essential elements levels (Cu, Zn, Mn and Fe) were measured in brain tissue along with histological analyses. External and internal dose-response analyses were performed using PROASTweb 70.1 software. The results have shown that subacute exposure to very low doses of Pb resulted in memory deficits in rats that was accompanied with acetylcholinesterase enzyme activity decrease. The observed hyperactive behaviour was accompanied by dose-dependent induction of brain oxidative stress and Zn elevation. The histological alterations in Purkinje cells were only detected in the group treated with the highest Pb dose. The lowest BMD considering entire oxidative status was calculated based on total oxidative status (4.5e-06 mg Pb/kg b.w./day). The findings reported in our study may be beneficial in further evaluating the health consequences and human health risk assessment of low-level Pb exposure.",
publisher = "Elsevier Ireland Ltd",
journal = "Chemico-Biological Interactions",
title = "Comprehensive insight into the neurotoxic mechanisms of low dose Pb exposure in Wistar rats: Benchmark dose analysis",
volume = "360",
doi = "10.1016/j.cbi.2022.109932"
}

Javorac, D., Anđelković, M., Repić, A., Tatović, S., Buha-Đorđević, A., Antonijević-Miljaković, E., Batinić, B., Boričić, N., Đukić-Ćosić, D., Antonijević, B.,& Bulat, Z.. (2022). Comprehensive insight into the neurotoxic mechanisms of low dose Pb exposure in Wistar rats: Benchmark dose analysis. in Chemico-Biological Interactions
Elsevier Ireland Ltd., 360.
https://doi.org/10.1016/j.cbi.2022.109932

Javorac D, Anđelković M, Repić A, Tatović S, Buha-Đorđević A, Antonijević-Miljaković E, Batinić B, Boričić N, Đukić-Ćosić D, Antonijević B, Bulat Z. Comprehensive insight into the neurotoxic mechanisms of low dose Pb exposure in Wistar rats: Benchmark dose analysis. in Chemico-Biological Interactions. 2022;360.
doi:10.1016/j.cbi.2022.109932 .

Javorac, Dragana, Anđelković, Milena, Repić, Aleksandra, Tatović, Simona, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Batinić, Bojan, Boričić, Novica, Đukić-Ćosić, Danijela, Antonijević, Biljana, Bulat, Zorica, "Comprehensive insight into the neurotoxic mechanisms of low dose Pb exposure in Wistar rats: Benchmark dose analysis" in Chemico-Biological Interactions, 360 (2022),
https://doi.org/10.1016/j.cbi.2022.109932 . .