TY  - JOUR
AU  - Bajić, Vladan
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Kotur-Stevuljević, Jelena
AU  - Isenović, Esma
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana
AU  - Lazić, Vesna
AU  - Ahrenkiel, S. Phillip
AU  - Nedeljković, Jovan M.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2968
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.
PB  - Elsevier Science BV, Amsterdam
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 

@article{
author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Pirković, Andrea and Kotur-Stevuljević, Jelena and Isenović, Esma and Sredojević, Dušan and Vukoje, Ivana and Lazić, Vesna and Ahrenkiel, S. Phillip and Nedeljković, Jovan M.",
year = "2017",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}

Bajić, V., Potparević, B., Živković, L., Pirković, A., Kotur-Stevuljević, J., Isenović, E., Sredojević, D., Vukoje, I., Lazić, V., Ahrenkiel, S. P.,& Nedeljković, J. M.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces
Elsevier Science BV, Amsterdam., 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032

Bajić V, Potparević B, Živković L, Pirković A, Kotur-Stevuljević J, Isenović E, Sredojević D, Vukoje I, Lazić V, Ahrenkiel SP, Nedeljković JM. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces. 2017;155:323-331.
doi:10.1016/j.colsurfb.2017.04.032 .

Bajić, Vladan, Potparević, Biljana, Živković, Lada, Pirković, Andrea, Kotur-Stevuljević, Jelena, Isenović, Esma, Sredojević, Dušan, Vukoje, Ivana, Lazić, Vesna, Ahrenkiel, S. Phillip, Nedeljković, Jovan M., "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 . .