TY  - JOUR
AU  - Vukašinović, Mila
AU  - Pantelić, Ivana
AU  - Savić, Sanela
AU  - Cekić, Nebojša
AU  - Vukašinović Sekulić, Maja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Tošić, Anđela
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5311
AB  - Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.
PB  - MDPI
T2  - Cosmetics
T1  - Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety
VL  - 10
IS  - 6
DO  - 10.3390/cosmetics10060162
ER  - 

@article{
author = "Vukašinović, Mila and Pantelić, Ivana and Savić, Sanela and Cekić, Nebojša and Vukašinović Sekulić, Maja and Antić-Stanković, Jelena and Božić, Dragana and Tošić, Anđela and Tamburić, Slobodanka and Savić, Snežana",
year = "2023",
abstract = "Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.",
publisher = "MDPI",
journal = "Cosmetics",
title = "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety",
volume = "10",
number = "6",
doi = "10.3390/cosmetics10060162"
}

Vukašinović, M., Pantelić, I., Savić, S., Cekić, N., Vukašinović Sekulić, M., Antić-Stanković, J., Božić, D., Tošić, A., Tamburić, S.,& Savić, S.. (2023). Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics
MDPI., 10(6).
https://doi.org/10.3390/cosmetics10060162

Vukašinović M, Pantelić I, Savić S, Cekić N, Vukašinović Sekulić M, Antić-Stanković J, Božić D, Tošić A, Tamburić S, Savić S. Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics. 2023;10(6).
doi:10.3390/cosmetics10060162 .

Vukašinović, Mila, Pantelić, Ivana, Savić, Sanela, Cekić, Nebojša, Vukašinović Sekulić, Maja, Antić-Stanković, Jelena, Božić, Dragana, Tošić, Anđela, Tamburić, Slobodanka, Savić, Snežana, "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety" in Cosmetics, 10, no. 6 (2023),
https://doi.org/10.3390/cosmetics10060162 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4300
AB  - The aim of this study was to assess and improve the oxidative stability of red raspberry seed oil–RO, a potential topical ingredient derived from food industry by-products, on its own and when incorporated in low energy nanoemulsion (NE). The RO’s oxidative stability was assessed at 5, 25, and 40 °C during one month of storage and expressed in: peroxide value, p-anisidine, and thiobarbituric reactive substances—TBARS value, while for NEs, lipid hydroperoxides and TBARS values were monitored. Both synthetic (butylated hydroxytoluene—BHT and ethylenediaminetetraacetic acid—EDTA), and natural (oregano essential oil—ORE and oak fruit extract—OAK) antioxidants were used. Pure RO and RO with BHT or ORE were stable at 5 °C and 25 °C, but at 40 °C BHT showed only moderate protection, while ORE was prooxidant. NEs prepared with new biodegradable polyglycerol esters-based surfactants, with droplet sizes of < 50 nm and narrow size distribution, showed improved physicochemical stability at room temperature, and especially at 40 °C, compared to NEs with polysorbate 80, which required the addition of antioxidants to preserve their stability. Natural antioxidants ORE and OAK were compatible with all NEs; therefore, their use is proposed as an effective alternative to synthetic antioxidants.
PB  - MDPI
T2  - Antioxidants
T1  - Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability
VL  - 11
IS  - 10
DO  - 10.3390/antiox11101898
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Tamburić, Slobodanka and Savić, Snežana",
year = "2022",
abstract = "The aim of this study was to assess and improve the oxidative stability of red raspberry seed oil–RO, a potential topical ingredient derived from food industry by-products, on its own and when incorporated in low energy nanoemulsion (NE). The RO’s oxidative stability was assessed at 5, 25, and 40 °C during one month of storage and expressed in: peroxide value, p-anisidine, and thiobarbituric reactive substances—TBARS value, while for NEs, lipid hydroperoxides and TBARS values were monitored. Both synthetic (butylated hydroxytoluene—BHT and ethylenediaminetetraacetic acid—EDTA), and natural (oregano essential oil—ORE and oak fruit extract—OAK) antioxidants were used. Pure RO and RO with BHT or ORE were stable at 5 °C and 25 °C, but at 40 °C BHT showed only moderate protection, while ORE was prooxidant. NEs prepared with new biodegradable polyglycerol esters-based surfactants, with droplet sizes of < 50 nm and narrow size distribution, showed improved physicochemical stability at room temperature, and especially at 40 °C, compared to NEs with polysorbate 80, which required the addition of antioxidants to preserve their stability. Natural antioxidants ORE and OAK were compatible with all NEs; therefore, their use is proposed as an effective alternative to synthetic antioxidants.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability",
volume = "11",
number = "10",
doi = "10.3390/antiox11101898"
}

Gledović, A., Janošević-Ležaić, A., Tamburić, S.,& Savić, S.. (2022). Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability. in Antioxidants
MDPI., 11(10).
https://doi.org/10.3390/antiox11101898

Gledović A, Janošević-Ležaić A, Tamburić S, Savić S. Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability. in Antioxidants. 2022;11(10).
doi:10.3390/antiox11101898 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Tamburić, Slobodanka, Savić, Snežana, "Red Raspberry Seed Oil Low Energy Nanoemulsions: Influence of Surfactants, Antioxidants, and Temperature on Oxidative Stability" in Antioxidants, 11, no. 10 (2022),
https://doi.org/10.3390/antiox11101898 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Nikolić, Ines
AU  - Tasić-Kostov, Marija
AU  - Antić-Stanković, Jelena
AU  - Krstonošić, Veljko
AU  - Ranđelović, Danijela
AU  - Božić, Dragana
AU  - Ilić, Dušan
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3777
AB  - This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.
PB  - MDPI AG
T2  - Nanomaterials
T1  - Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance
VL  - 11
IS  - 1
SP  - 1
EP  - 21
DO  - 10.3390/nano11010217
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Nikolić, Ines and Tasić-Kostov, Marija and Antić-Stanković, Jelena and Krstonošić, Veljko and Ranđelović, Danijela and Božić, Dragana and Ilić, Dušan and Tamburić, Slobodanka and Savić, Snežana",
year = "2021",
abstract = "This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.",
publisher = "MDPI AG",
journal = "Nanomaterials",
title = "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance",
volume = "11",
number = "1",
pages = "1-21",
doi = "10.3390/nano11010217"
}

Gledović, A., Janošević-Ležaić, A., Nikolić, I., Tasić-Kostov, M., Antić-Stanković, J., Krstonošić, V., Ranđelović, D., Božić, D., Ilić, D., Tamburić, S.,& Savić, S.. (2021). Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials
MDPI AG., 11(1), 1-21.
https://doi.org/10.3390/nano11010217

Gledović A, Janošević-Ležaić A, Nikolić I, Tasić-Kostov M, Antić-Stanković J, Krstonošić V, Ranđelović D, Božić D, Ilić D, Tamburić S, Savić S. Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials. 2021;11(1):1-21.
doi:10.3390/nano11010217 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Nikolić, Ines, Tasić-Kostov, Marija, Antić-Stanković, Jelena, Krstonošić, Veljko, Ranđelović, Danijela, Božić, Dragana, Ilić, Dušan, Tamburić, Slobodanka, Savić, Snežana, "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance" in Nanomaterials, 11, no. 1 (2021):1-21,
https://doi.org/10.3390/nano11010217 . .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Krstonošić, Veljko
AU  - Đoković, Jelena
AU  - Nikolić, Ines
AU  - Bajuk-Bogdanović, Danica
AU  - Antić-Stanković, Jelena
AU  - Ranđelović, Danijela
AU  - Savić, Sanela M.
AU  - Filipović, Mila
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3581
AB  - Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity
VL  - 15
IS  - 4
DO  - 10.1371/journal.pone.0230993
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Krstonošić, Veljko and Đoković, Jelena and Nikolić, Ines and Bajuk-Bogdanović, Danica and Antić-Stanković, Jelena and Ranđelović, Danijela and Savić, Sanela M. and Filipović, Mila and Tamburić, Slobodanka and Savić, Snežana",
year = "2020",
abstract = "Considering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2- extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram - PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity",
volume = "15",
number = "4",
doi = "10.1371/journal.pone.0230993"
}

Gledović, A., Janošević-Ležaić, A., Krstonošić, V., Đoković, J., Nikolić, I., Bajuk-Bogdanović, D., Antić-Stanković, J., Ranđelović, D., Savić, S. M., Filipović, M., Tamburić, S.,& Savić, S.. (2020). Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE
Public Library of Science., 15(4).
https://doi.org/10.1371/journal.pone.0230993

Gledović A, Janošević-Ležaić A, Krstonošić V, Đoković J, Nikolić I, Bajuk-Bogdanović D, Antić-Stanković J, Ranđelović D, Savić SM, Filipović M, Tamburić S, Savić S. Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity. in PLoS ONE. 2020;15(4).
doi:10.1371/journal.pone.0230993 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Krstonošić, Veljko, Đoković, Jelena, Nikolić, Ines, Bajuk-Bogdanović, Danica, Antić-Stanković, Jelena, Ranđelović, Danijela, Savić, Sanela M., Filipović, Mila, Tamburić, Slobodanka, Savić, Snežana, "Low-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activity" in PLoS ONE, 15, no. 4 (2020),
https://doi.org/10.1371/journal.pone.0230993 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Lukić, Milica
AU  - Pantelić, Ivana
AU  - Reichl, Stephan
AU  - Tamburić, Slobodanka
AU  - Mueller-Goymann, Christel
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1533
AB  - To be considered as a suitable vehicle for drugs/cosmetic actives, an emulsion system should have a number of desirable properties mainly dependent on surfactant used for its stabilization. In the current study, C12-14 alkyl polyglucoside (APG)-mixed emulsifier of natural origin has been investigated in a series of binary (emulsifier concentration 10-25% (w/w)) and ternary systems with fixed emulsifier content (15% (w/w)) with or without glycerol. To elucidate the systems' colloidal structure the following physicochemical techniques were employed: polarization and transmission electron microscopy, X-ray diffraction (WAXD and SAXD), thermal analysis (DSC and TGA), complex rheological, pH, and conductivity measurements. Additionally, the emulsion vehicles' skin hydration potential was tested in vivo, on human skin under occlusion. In a series of binary systems with fixed emulsifier/water ratios ranging from 10/90 to 25/75 the predominance of a lamellar mesophase was found, changing its character from a liquid crystalline to a gel crystalline type. The same was observed in gel emulsions containing equal amounts of emulsifier and oil (15% (w/w)), but varying in glycerol content (0-25%). Different emulsion samples exhibited different water distribution modes in the structure, reflecting their rheological behavior and also their skin hydration capacity.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Colloid and Interface Science
T1  - An alkyl polyglucoside-mixed emulsifier as stabilizer of emulsion systems: The influence of colloidal structure on emulsions skin hydration potential
VL  - 358
IS  - 1
SP  - 182
EP  - 191
DO  - 10.1016/j.jcis.2011.02.049
ER  - 

@article{
author = "Savić, Snežana and Lukić, Milica and Pantelić, Ivana and Reichl, Stephan and Tamburić, Slobodanka and Mueller-Goymann, Christel",
year = "2011",
abstract = "To be considered as a suitable vehicle for drugs/cosmetic actives, an emulsion system should have a number of desirable properties mainly dependent on surfactant used for its stabilization. In the current study, C12-14 alkyl polyglucoside (APG)-mixed emulsifier of natural origin has been investigated in a series of binary (emulsifier concentration 10-25% (w/w)) and ternary systems with fixed emulsifier content (15% (w/w)) with or without glycerol. To elucidate the systems' colloidal structure the following physicochemical techniques were employed: polarization and transmission electron microscopy, X-ray diffraction (WAXD and SAXD), thermal analysis (DSC and TGA), complex rheological, pH, and conductivity measurements. Additionally, the emulsion vehicles' skin hydration potential was tested in vivo, on human skin under occlusion. In a series of binary systems with fixed emulsifier/water ratios ranging from 10/90 to 25/75 the predominance of a lamellar mesophase was found, changing its character from a liquid crystalline to a gel crystalline type. The same was observed in gel emulsions containing equal amounts of emulsifier and oil (15% (w/w)), but varying in glycerol content (0-25%). Different emulsion samples exhibited different water distribution modes in the structure, reflecting their rheological behavior and also their skin hydration capacity.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Colloid and Interface Science",
title = "An alkyl polyglucoside-mixed emulsifier as stabilizer of emulsion systems: The influence of colloidal structure on emulsions skin hydration potential",
volume = "358",
number = "1",
pages = "182-191",
doi = "10.1016/j.jcis.2011.02.049"
}

Savić, S., Lukić, M., Pantelić, I., Reichl, S., Tamburić, S.,& Mueller-Goymann, C.. (2011). An alkyl polyglucoside-mixed emulsifier as stabilizer of emulsion systems: The influence of colloidal structure on emulsions skin hydration potential. in Journal of Colloid and Interface Science
Academic Press Inc Elsevier Science, San Diego., 358(1), 182-191.
https://doi.org/10.1016/j.jcis.2011.02.049

Savić S, Lukić M, Pantelić I, Reichl S, Tamburić S, Mueller-Goymann C. An alkyl polyglucoside-mixed emulsifier as stabilizer of emulsion systems: The influence of colloidal structure on emulsions skin hydration potential. in Journal of Colloid and Interface Science. 2011;358(1):182-191.
doi:10.1016/j.jcis.2011.02.049 .

Savić, Snežana, Lukić, Milica, Pantelić, Ivana, Reichl, Stephan, Tamburić, Slobodanka, Mueller-Goymann, Christel, "An alkyl polyglucoside-mixed emulsifier as stabilizer of emulsion systems: The influence of colloidal structure on emulsions skin hydration potential" in Journal of Colloid and Interface Science, 358, no. 1 (2011):182-191,
https://doi.org/10.1016/j.jcis.2011.02.049 . .

TY  - CHAP
AU  - Savić, Snežana
AU  - Tamburić, Slobodanka
AU  - Jančić, Biljana
AU  - Milić, Jela
AU  - Vuleta, Gordana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1578
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Colloids in Cosmetics and Personal Care
T1  - The Impact of Urea on the Colloidal Structure of Alkylpolyglucoside-Based Emulsions: Physicochemical and In Vitro In Vivo Characterization
VL  - 4
SP  - 259
EP  - 274
DO  - 10.1002/9783527631131.ch12
ER  - 

@inbook{
author = "Savić, Snežana and Tamburić, Slobodanka and Jančić, Biljana and Milić, Jela and Vuleta, Gordana",
year = "2011",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Colloids in Cosmetics and Personal Care",
booktitle = "The Impact of Urea on the Colloidal Structure of Alkylpolyglucoside-Based Emulsions: Physicochemical and In Vitro In Vivo Characterization",
volume = "4",
pages = "259-274",
doi = "10.1002/9783527631131.ch12"
}

Savić, S., Tamburić, S., Jančić, B., Milić, J.,& Vuleta, G.. (2011). The Impact of Urea on the Colloidal Structure of Alkylpolyglucoside-Based Emulsions: Physicochemical and In Vitro In Vivo Characterization. in Colloids in Cosmetics and Personal Care
Wiley-VCH Verlag GMBH, Weinheim., 4, 259-274.
https://doi.org/10.1002/9783527631131.ch12

Savić S, Tamburić S, Jančić B, Milić J, Vuleta G. The Impact of Urea on the Colloidal Structure of Alkylpolyglucoside-Based Emulsions: Physicochemical and In Vitro In Vivo Characterization. in Colloids in Cosmetics and Personal Care. 2011;4:259-274.
doi:10.1002/9783527631131.ch12 .

Savić, Snežana, Tamburić, Slobodanka, Jančić, Biljana, Milić, Jela, Vuleta, Gordana, "The Impact of Urea on the Colloidal Structure of Alkylpolyglucoside-Based Emulsions: Physicochemical and In Vitro In Vivo Characterization" in Colloids in Cosmetics and Personal Care, 4 (2011):259-274,
https://doi.org/10.1002/9783527631131.ch12 . .

TY  - JOUR
AU  - Tasić-Kostov, Marija
AU  - Savić, Snežana
AU  - Lukić, Milica
AU  - Tamburić, Slobodanka
AU  - Pavlović, Miloš
AU  - Vuleta, Gordana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1393
AB  - Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of alpha-hydroxyacids (AHAs), showing advantages over them. The aim of part I of this study was to compare efficacy and irritation potential of LA vs. glycolic acid (GA) from two types of vehicles - gel and emulsion. In part II, effects of LA-containing emulsions based on a new, natural emulsifier of alkylpolyglucoside (APG) type were evaluated. Methods Skin bioengineering was used on 77 healthy volunteers to assess: color as erythema and melanin (MI) index, transepidermal water loss, electrical capacitance and pH of the skin. In part I of the study, the parameters were measured after occlusion and periodically during 2 weeks of test samples application; in part II parameters were measured periodically during 4 weeks. Results/conclusion LA-containing samples has produced better skin performance when compared with corresponding GA-containing ones, particularly the lack of both skin irritation and skin barrier impairment. When used in vehicles based on a new APG-emulsifier, LA and GA have shown better efficacy, emphasizing the importance of vehicle on the effects of topical actives. LA (6%) in the emulsion based on APGs could be proposed as an alternative to low-molecular AHAs in cosmeceuticals.
PB  - Wiley Periodicals, Inc, Malden
T2  - Journal of Cosmetic Dermatology
T1  - Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid
VL  - 9
IS  - 1
SP  - 3
EP  - 10
DO  - 10.1111/j.1473-2165.2010.00474.x
ER  - 

@article{
author = "Tasić-Kostov, Marija and Savić, Snežana and Lukić, Milica and Tamburić, Slobodanka and Pavlović, Miloš and Vuleta, Gordana",
year = "2010",
abstract = "Background/aims Lactobionic acid (LA) is a newer cosmeceutical active belonging to the class of alpha-hydroxyacids (AHAs), showing advantages over them. The aim of part I of this study was to compare efficacy and irritation potential of LA vs. glycolic acid (GA) from two types of vehicles - gel and emulsion. In part II, effects of LA-containing emulsions based on a new, natural emulsifier of alkylpolyglucoside (APG) type were evaluated. Methods Skin bioengineering was used on 77 healthy volunteers to assess: color as erythema and melanin (MI) index, transepidermal water loss, electrical capacitance and pH of the skin. In part I of the study, the parameters were measured after occlusion and periodically during 2 weeks of test samples application; in part II parameters were measured periodically during 4 weeks. Results/conclusion LA-containing samples has produced better skin performance when compared with corresponding GA-containing ones, particularly the lack of both skin irritation and skin barrier impairment. When used in vehicles based on a new APG-emulsifier, LA and GA have shown better efficacy, emphasizing the importance of vehicle on the effects of topical actives. LA (6%) in the emulsion based on APGs could be proposed as an alternative to low-molecular AHAs in cosmeceuticals.",
publisher = "Wiley Periodicals, Inc, Malden",
journal = "Journal of Cosmetic Dermatology",
title = "Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid",
volume = "9",
number = "1",
pages = "3-10",
doi = "10.1111/j.1473-2165.2010.00474.x"
}

Tasić-Kostov, M., Savić, S., Lukić, M., Tamburić, S., Pavlović, M.,& Vuleta, G.. (2010). Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid. in Journal of Cosmetic Dermatology
Wiley Periodicals, Inc, Malden., 9(1), 3-10.
https://doi.org/10.1111/j.1473-2165.2010.00474.x

Tasić-Kostov M, Savić S, Lukić M, Tamburić S, Pavlović M, Vuleta G. Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid. in Journal of Cosmetic Dermatology. 2010;9(1):3-10.
doi:10.1111/j.1473-2165.2010.00474.x .

Tasić-Kostov, Marija, Savić, Snežana, Lukić, Milica, Tamburić, Slobodanka, Pavlović, Miloš, Vuleta, Gordana, "Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and efficacy aspects in comparison to glycolic acid" in Journal of Cosmetic Dermatology, 9, no. 1 (2010):3-10,
https://doi.org/10.1111/j.1473-2165.2010.00474.x . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Tamburić, Slobodanka
AU  - Savić, Miroslav
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1373
AB  - Importance of the field: Surfactants play an important role in the development of both conventional and advanced (colloidal) drug delivery systems. There are several commercial surfactants, but a proportionally small group of them is approved as pharmaceutical excipients, recognized in various pharmacopoeias and therefore widely accepted by the pharmaceutical industry. Areas covered in this review: The review covers some of the main categories of natural, sugar-based surfactants (alkyl polyglucosides and sugar esters) as prospective pharmaceutical excipients. It provides analysis of the physicochemical characteristics of sugar-based surfactants and their possible roles in the design of conventional or advanced drug delivery systems for different routes of administration. What the reader will gain: Summary and analysis of recent data on functionality, applied concentrations and formulation improvements produced by alkyl polyglucosides and sugar esters in different conventional and advanced delivery systems could be of interest to researchers dealing with drug formulation. Take home message: Recent FDA certification of an alkyl polyglucoside surfactant for topical formulation presents a significant step in the process of recognition of this relatively new group of surfactants. This could trigger further research into the potential benefits of naturally derived materials in both conventional and new drug delivery systems.
PB  - Informa Healthcare, London
T2  - Expert Opinion on Drug Delivery
T1  - From conventional towards new - natural surfactants in drug delivery systems design: current status and perspectives
VL  - 7
IS  - 3
SP  - 353
EP  - 369
DO  - 10.1517/17425240903535833
ER  - 

@article{
author = "Savić, Snežana and Tamburić, Slobodanka and Savić, Miroslav",
year = "2010",
abstract = "Importance of the field: Surfactants play an important role in the development of both conventional and advanced (colloidal) drug delivery systems. There are several commercial surfactants, but a proportionally small group of them is approved as pharmaceutical excipients, recognized in various pharmacopoeias and therefore widely accepted by the pharmaceutical industry. Areas covered in this review: The review covers some of the main categories of natural, sugar-based surfactants (alkyl polyglucosides and sugar esters) as prospective pharmaceutical excipients. It provides analysis of the physicochemical characteristics of sugar-based surfactants and their possible roles in the design of conventional or advanced drug delivery systems for different routes of administration. What the reader will gain: Summary and analysis of recent data on functionality, applied concentrations and formulation improvements produced by alkyl polyglucosides and sugar esters in different conventional and advanced delivery systems could be of interest to researchers dealing with drug formulation. Take home message: Recent FDA certification of an alkyl polyglucoside surfactant for topical formulation presents a significant step in the process of recognition of this relatively new group of surfactants. This could trigger further research into the potential benefits of naturally derived materials in both conventional and new drug delivery systems.",
publisher = "Informa Healthcare, London",
journal = "Expert Opinion on Drug Delivery",
title = "From conventional towards new - natural surfactants in drug delivery systems design: current status and perspectives",
volume = "7",
number = "3",
pages = "353-369",
doi = "10.1517/17425240903535833"
}

Savić, S., Tamburić, S.,& Savić, M.. (2010). From conventional towards new - natural surfactants in drug delivery systems design: current status and perspectives. in Expert Opinion on Drug Delivery
Informa Healthcare, London., 7(3), 353-369.
https://doi.org/10.1517/17425240903535833

Savić S, Tamburić S, Savić M. From conventional towards new - natural surfactants in drug delivery systems design: current status and perspectives. in Expert Opinion on Drug Delivery. 2010;7(3):353-369.
doi:10.1517/17425240903535833 .

Savić, Snežana, Tamburić, Slobodanka, Savić, Miroslav, "From conventional towards new - natural surfactants in drug delivery systems design: current status and perspectives" in Expert Opinion on Drug Delivery, 7, no. 3 (2010):353-369,
https://doi.org/10.1517/17425240903535833 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Weber, Christian
AU  - Tamburić, Slobodanka
AU  - Savić, Miroslav
AU  - Mueller-Goymann, Christel
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1272
AB  - There is a growing need for in-depth research into new skill- and environment-friendly surfactants, such as alkylpolyglucosides. The aim of this study was to assess whether, to which extent and by what mechanism the two commonly used hydrophilic excipients, propylene glycol (PG) and glycerol (GL), affect the Colloidal structure of emulsions formed by a natural mixed emulsifier, cetearyl glucoside and cetearyl alcohol. Furthermore, the study was concerned with the effect of these changes on in vitro permeation profiles of two model drugs (diclofenac sodium and caffeine) and in vivo skin performance of the test samples. The results have shown that the emulsion vehicles consisted of a complex colloidal structure of lamellar liquid crystalline and lamellar gel crystalline type. PG addition produced a stronger hydrophilic lamellar gel phase than GL, which was independent on the model drug used. PG-containing vehicles have revealed a considerable amount of interlamellar PG/water mixture, with incorporated drug. In vitro permeation data obtained using artificial skill constructs (ASC) confirmed the relationship between rheological profiles of vehicles and the extent of skill delivery. Higher permeation profiles of both drugs from PG-containing formulations coincided with a higher increase in transepidermal water loss observed in in vivo study on human volunteers, which confirms the penetration/permeation enhancer effect of PG. It also indicates the existence of the vehicle/ASC interactions analogous to those between the vehicle and the skin, thus affirming the use of ASC as a reliable tool for permeation studies. Contrary to the effect of PG, the results obtained with GL suggest that it may have a permeation-retarding rather than a permeation-enhancing effect ill topical vehicles of this type.
PB  - Elsevier Science Inc, New York
T2  - Journal of Pharmaceutical Sciences
T1  - Topical Vehicles Based on Natural Surfactant/Fatty Alcohols Mixed Emulsifier: The Influence of Two Polyols on the Colloidal Structure and In Vitro/In Vivo Skin Performance
VL  - 98
IS  - 6
SP  - 2073
EP  - 2090
DO  - 10.1002/jps.21591
ER  - 

@article{
author = "Savić, Snežana and Weber, Christian and Tamburić, Slobodanka and Savić, Miroslav and Mueller-Goymann, Christel",
year = "2009",
abstract = "There is a growing need for in-depth research into new skill- and environment-friendly surfactants, such as alkylpolyglucosides. The aim of this study was to assess whether, to which extent and by what mechanism the two commonly used hydrophilic excipients, propylene glycol (PG) and glycerol (GL), affect the Colloidal structure of emulsions formed by a natural mixed emulsifier, cetearyl glucoside and cetearyl alcohol. Furthermore, the study was concerned with the effect of these changes on in vitro permeation profiles of two model drugs (diclofenac sodium and caffeine) and in vivo skin performance of the test samples. The results have shown that the emulsion vehicles consisted of a complex colloidal structure of lamellar liquid crystalline and lamellar gel crystalline type. PG addition produced a stronger hydrophilic lamellar gel phase than GL, which was independent on the model drug used. PG-containing vehicles have revealed a considerable amount of interlamellar PG/water mixture, with incorporated drug. In vitro permeation data obtained using artificial skill constructs (ASC) confirmed the relationship between rheological profiles of vehicles and the extent of skill delivery. Higher permeation profiles of both drugs from PG-containing formulations coincided with a higher increase in transepidermal water loss observed in in vivo study on human volunteers, which confirms the penetration/permeation enhancer effect of PG. It also indicates the existence of the vehicle/ASC interactions analogous to those between the vehicle and the skin, thus affirming the use of ASC as a reliable tool for permeation studies. Contrary to the effect of PG, the results obtained with GL suggest that it may have a permeation-retarding rather than a permeation-enhancing effect ill topical vehicles of this type.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Pharmaceutical Sciences",
title = "Topical Vehicles Based on Natural Surfactant/Fatty Alcohols Mixed Emulsifier: The Influence of Two Polyols on the Colloidal Structure and In Vitro/In Vivo Skin Performance",
volume = "98",
number = "6",
pages = "2073-2090",
doi = "10.1002/jps.21591"
}

Savić, S., Weber, C., Tamburić, S., Savić, M.,& Mueller-Goymann, C.. (2009). Topical Vehicles Based on Natural Surfactant/Fatty Alcohols Mixed Emulsifier: The Influence of Two Polyols on the Colloidal Structure and In Vitro/In Vivo Skin Performance. in Journal of Pharmaceutical Sciences
Elsevier Science Inc, New York., 98(6), 2073-2090.
https://doi.org/10.1002/jps.21591

Savić S, Weber C, Tamburić S, Savić M, Mueller-Goymann C. Topical Vehicles Based on Natural Surfactant/Fatty Alcohols Mixed Emulsifier: The Influence of Two Polyols on the Colloidal Structure and In Vitro/In Vivo Skin Performance. in Journal of Pharmaceutical Sciences. 2009;98(6):2073-2090.
doi:10.1002/jps.21591 .

Savić, Snežana, Weber, Christian, Tamburić, Slobodanka, Savić, Miroslav, Mueller-Goymann, Christel, "Topical Vehicles Based on Natural Surfactant/Fatty Alcohols Mixed Emulsifier: The Influence of Two Polyols on the Colloidal Structure and In Vitro/In Vivo Skin Performance" in Journal of Pharmaceutical Sciences, 98, no. 6 (2009):2073-2090,
https://doi.org/10.1002/jps.21591 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Tamburić, Slobodanka
AU  - Kovacević, Anđelka
AU  - Daniels, Rolf
AU  - Mueller-Goymann, Christel
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1034
AB  - There is a growing need for research into new skin- and environment-friendly surfactants. The aim of the study was to find out whether a combination of an alkylpolyglucoside natural surfactant with established pharmaceutical excipients could provide a solid pharmaceutical base with satisfied physical stability. The study was carried out in two phases: the first one focused on the colloidal structure of vehicles formulated with oils of different polarity and/or different costabilizer (lipophilic versus hydrophilic) and the second one evaluated vehicles' physical stability. A number of techniques were used (polarization, light, and transmission electron microscopy, pH, conductivity and thermogravimetric measurements, rheological analysis and cyclic temperature stress test). Natural surfactant's interaction with used excipients resulted in the formation of semisolid emulsion systems of different rheological profiles, stabilized predominantly by synergistic effects of lamellar liquid-crystalline (L alpha) and complex lamellar gel (L beta) phases. The type of used oil and costabilizer significantly influenced the colloidal structure of the vehicles, particularly in terms of water distribution mode and initial rheological performance as well as their physical stability. It was recommended that medium polar oils of ester type and lipophilic costabilizers, particularly long chain fatty alcohols, should be used in the formulation of stable alkylpolyglucoside-based topical vehicles.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Dispersion Science and Technology
T1  - Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability
VL  - 29
IS  - 9
SP  - 1276
EP  - 1287
DO  - 10.1080/01932690701857558
ER  - 

@article{
author = "Savić, Snežana and Tamburić, Slobodanka and Kovacević, Anđelka and Daniels, Rolf and Mueller-Goymann, Christel",
year = "2008",
abstract = "There is a growing need for research into new skin- and environment-friendly surfactants. The aim of the study was to find out whether a combination of an alkylpolyglucoside natural surfactant with established pharmaceutical excipients could provide a solid pharmaceutical base with satisfied physical stability. The study was carried out in two phases: the first one focused on the colloidal structure of vehicles formulated with oils of different polarity and/or different costabilizer (lipophilic versus hydrophilic) and the second one evaluated vehicles' physical stability. A number of techniques were used (polarization, light, and transmission electron microscopy, pH, conductivity and thermogravimetric measurements, rheological analysis and cyclic temperature stress test). Natural surfactant's interaction with used excipients resulted in the formation of semisolid emulsion systems of different rheological profiles, stabilized predominantly by synergistic effects of lamellar liquid-crystalline (L alpha) and complex lamellar gel (L beta) phases. The type of used oil and costabilizer significantly influenced the colloidal structure of the vehicles, particularly in terms of water distribution mode and initial rheological performance as well as their physical stability. It was recommended that medium polar oils of ester type and lipophilic costabilizers, particularly long chain fatty alcohols, should be used in the formulation of stable alkylpolyglucoside-based topical vehicles.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Dispersion Science and Technology",
title = "Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability",
volume = "29",
number = "9",
pages = "1276-1287",
doi = "10.1080/01932690701857558"
}

Savić, S., Tamburić, S., Kovacević, A., Daniels, R.,& Mueller-Goymann, C.. (2008). Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability. in Journal of Dispersion Science and Technology
Taylor & Francis Inc, Philadelphia., 29(9), 1276-1287.
https://doi.org/10.1080/01932690701857558

Savić S, Tamburić S, Kovacević A, Daniels R, Mueller-Goymann C. Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability. in Journal of Dispersion Science and Technology. 2008;29(9):1276-1287.
doi:10.1080/01932690701857558 .

Savić, Snežana, Tamburić, Slobodanka, Kovacević, Anđelka, Daniels, Rolf, Mueller-Goymann, Christel, "Natural Surfactant-Based Emulsion Systems: The Influence of Common Pharmaceutical Excipients on Colloidal Structure and Physical Stability" in Journal of Dispersion Science and Technology, 29, no. 9 (2008):1276-1287,
https://doi.org/10.1080/01932690701857558 . .

TY  - JOUR
AU  - Savić, Snežana
AU  - Savić, M.
AU  - Tamburić, Slobodanka
AU  - Vuleta, G.
AU  - Vesić, Sonja
AU  - Mueller-Goymann, Christel
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/939
AB  - There is a growing need for research into new skin- and environment-friendly surfactants. This paper focuses on a natural surfactant of an alkylpolyglucoside type, which can form both thermotropic and lyotropic liquid-crystalline phases. The aim of this study was to relate some physicochemical properties (characterised by polarisation and transmission electron microscopy, thermal analysis and rheology) of the three formulations based on cetearyl glucoside and cetearyl alcohol, to the results of in vitro and in vivo bioavailability of hydrocortisone (HC). The three formulations contained oils of different polarity (medium chain triglycerides: MG, isopropyl myristate: IPM and light liquid paraffin: LP), respectively In vitro permeation was followed through the artificial skin constructs (ASC), while the parameters measured in vivo were erythema index: EI (using instrumental human skin blanching assay), transepidermal water loss (TEWL) and stratum corneum. hydration (SCH). The vehicles based on cetearyl glucoside and cetearyl alcohol showed a complex colloidal structure of lamellar liquid-crystalline and lamellar gel-crystalline type, depending on oil polarity. Rheological profile of the vehicle was directly related to the in vitro profile of the HC permeation. In vivo results suggested that the vehicle with MG retarded the HC permeation, whereas less polar IPM and non-polar LP enhanced it. It is suggested that the enhancement is achieved either by a direct interaction with lipid lamellae of the SC or indirectly by improving skin hydration. There were no adverse effects during in vivo study, which indicates a good safety profile of this alkylpolyglucoside surfactant.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation
VL  - 30
IS  - 5
SP  - 441
EP  - 450
DO  - 10.1016/j.ejps.2007.01.006
ER  - 

@article{
author = "Savić, Snežana and Savić, M. and Tamburić, Slobodanka and Vuleta, G. and Vesić, Sonja and Mueller-Goymann, Christel",
year = "2007",
abstract = "There is a growing need for research into new skin- and environment-friendly surfactants. This paper focuses on a natural surfactant of an alkylpolyglucoside type, which can form both thermotropic and lyotropic liquid-crystalline phases. The aim of this study was to relate some physicochemical properties (characterised by polarisation and transmission electron microscopy, thermal analysis and rheology) of the three formulations based on cetearyl glucoside and cetearyl alcohol, to the results of in vitro and in vivo bioavailability of hydrocortisone (HC). The three formulations contained oils of different polarity (medium chain triglycerides: MG, isopropyl myristate: IPM and light liquid paraffin: LP), respectively In vitro permeation was followed through the artificial skin constructs (ASC), while the parameters measured in vivo were erythema index: EI (using instrumental human skin blanching assay), transepidermal water loss (TEWL) and stratum corneum. hydration (SCH). The vehicles based on cetearyl glucoside and cetearyl alcohol showed a complex colloidal structure of lamellar liquid-crystalline and lamellar gel-crystalline type, depending on oil polarity. Rheological profile of the vehicle was directly related to the in vitro profile of the HC permeation. In vivo results suggested that the vehicle with MG retarded the HC permeation, whereas less polar IPM and non-polar LP enhanced it. It is suggested that the enhancement is achieved either by a direct interaction with lipid lamellae of the SC or indirectly by improving skin hydration. There were no adverse effects during in vivo study, which indicates a good safety profile of this alkylpolyglucoside surfactant.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation",
volume = "30",
number = "5",
pages = "441-450",
doi = "10.1016/j.ejps.2007.01.006"
}

Savić, S., Savić, M., Tamburić, S., Vuleta, G., Vesić, S.,& Mueller-Goymann, C.. (2007). An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 30(5), 441-450.
https://doi.org/10.1016/j.ejps.2007.01.006

Savić S, Savić M, Tamburić S, Vuleta G, Vesić S, Mueller-Goymann C. An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation. in European Journal of Pharmaceutical Sciences. 2007;30(5):441-450.
doi:10.1016/j.ejps.2007.01.006 .

Savić, Snežana, Savić, M., Tamburić, Slobodanka, Vuleta, G., Vesić, Sonja, Mueller-Goymann, Christel, "An alkylpolyglucoside surfactant as a prospective pharmaceutical excipient for topical formulations: The influence of oil polarity on the colloidal structure and hydrocortisone in vitro/in vivo permeation" in European Journal of Pharmaceutical Sciences, 30, no. 5 (2007):441-450,
https://doi.org/10.1016/j.ejps.2007.01.006 . .

TY  - CONF
AU  - Savić, Snežana
AU  - Vasiljević, Dragana
AU  - Tasić, M.
AU  - Tamburić, Slobodanka
AU  - Vuleta, Gordana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/722
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Formulation and physicochemical characterization of O/W cream vehicles for novel cosmetic active substance: Lactobionic acid
T1  - Formulacija i fizičkohemijska karakterizacija U/V kremova kao nosača za novu kozmetički aktivnu supstancu - laktobionsku kiselinu
VL  - 56
IS  - 4
SP  - 520
EP  - 521
UR  - https://hdl.handle.net/21.15107/rcub_farfar_722
ER  - 

@conference{
author = "Savić, Snežana and Vasiljević, Dragana and Tasić, M. and Tamburić, Slobodanka and Vuleta, Gordana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Formulation and physicochemical characterization of O/W cream vehicles for novel cosmetic active substance: Lactobionic acid, Formulacija i fizičkohemijska karakterizacija U/V kremova kao nosača za novu kozmetički aktivnu supstancu - laktobionsku kiselinu",
volume = "56",
number = "4",
pages = "520-521",
url = "https://hdl.handle.net/21.15107/rcub_farfar_722"
}

Savić, S., Vasiljević, D., Tasić, M., Tamburić, S.,& Vuleta, G.. (2006). Formulation and physicochemical characterization of O/W cream vehicles for novel cosmetic active substance: Lactobionic acid. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 520-521.
https://hdl.handle.net/21.15107/rcub_farfar_722

Savić S, Vasiljević D, Tasić M, Tamburić S, Vuleta G. Formulation and physicochemical characterization of O/W cream vehicles for novel cosmetic active substance: Lactobionic acid. in Arhiv za farmaciju. 2006;56(4):520-521.
https://hdl.handle.net/21.15107/rcub_farfar_722 .

Savić, Snežana, Vasiljević, Dragana, Tasić, M., Tamburić, Slobodanka, Vuleta, Gordana, "Formulation and physicochemical characterization of O/W cream vehicles for novel cosmetic active substance: Lactobionic acid" in Arhiv za farmaciju, 56, no. 4 (2006):520-521,
https://hdl.handle.net/21.15107/rcub_farfar_722 .

TY  - CONF
AU  - Savić, Snežana
AU  - Kovacević, Anđelka
AU  - Tamburić, Slobodanka
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Vuleta, Gordana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/738
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Study on sugar surfactants mesomorphic behavior in hydrous and anhydrous emulsion vehicles stabilization
T1  - Studija mezomorfnog ponašanja šećernih emulgatora u stabilizaciji vodenih i nevodenih emulzionih nosača
VL  - 56
IS  - 4
SP  - 522
EP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_farfar_738
ER  - 

@conference{
author = "Savić, Snežana and Kovacević, Anđelka and Tamburić, Slobodanka and Krajišnik, Danina and Milić, Jela and Vuleta, Gordana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Study on sugar surfactants mesomorphic behavior in hydrous and anhydrous emulsion vehicles stabilization, Studija mezomorfnog ponašanja šećernih emulgatora u stabilizaciji vodenih i nevodenih emulzionih nosača",
volume = "56",
number = "4",
pages = "522-523",
url = "https://hdl.handle.net/21.15107/rcub_farfar_738"
}

Savić, S., Kovacević, A., Tamburić, S., Krajišnik, D., Milić, J.,& Vuleta, G.. (2006). Study on sugar surfactants mesomorphic behavior in hydrous and anhydrous emulsion vehicles stabilization. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 522-523.
https://hdl.handle.net/21.15107/rcub_farfar_738

Savić S, Kovacević A, Tamburić S, Krajišnik D, Milić J, Vuleta G. Study on sugar surfactants mesomorphic behavior in hydrous and anhydrous emulsion vehicles stabilization. in Arhiv za farmaciju. 2006;56(4):522-523.
https://hdl.handle.net/21.15107/rcub_farfar_738 .

Savić, Snežana, Kovacević, Anđelka, Tamburić, Slobodanka, Krajišnik, Danina, Milić, Jela, Vuleta, Gordana, "Study on sugar surfactants mesomorphic behavior in hydrous and anhydrous emulsion vehicles stabilization" in Arhiv za farmaciju, 56, no. 4 (2006):522-523,
https://hdl.handle.net/21.15107/rcub_farfar_738 .

TY  - JOUR
AU  - Savić, Snežana
AU  - Tamburić, Slobodanka
AU  - Savić, Miroslav
AU  - Cekić, Nebojša
AU  - Milić, J.
AU  - Vuleta, G
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/528
AB  - It is known that, depending on the concentration, treatment with urea could improve skin barrier function, despite its penetration-enhancing properties. This controversial skin effect of urea has been explored systematically in this study in terms of the effect of vehicle on the performance of urea. In the first part, a series of four semi-solid emulsions with 5% (w/w) urea, varying in the type of emulsion, nature of emulsifier and polarity of oil ingredients, have been evaluated with regard to their skin hydrating and transepidermal water loss (TEWL)-modifying properties. Placebo samples were tested alongside the urea-containing ones. Two best performing moisturisers from the above were chosen for the second part of the study, in which sodium lauryl sulphate (SLS)-irritated skin was treated with both placebo and urea-containing samples. In addition to TEWL and skin hydration level, the erythema index (EI) was measured before, during and after the treatment. The results have shown that barrier-improving and hydrating abilities of urea are bi-directional and dependent on both the type of vehicle used for its delivery and the state of skin.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Vehicle-controlled effect of urea on normal and SLS-irritated skin
VL  - 271
IS  - 1-2
SP  - 269
EP  - 280
DO  - 10.1016/j.ijpharm.2003.11.033
ER  - 

@article{
author = "Savić, Snežana and Tamburić, Slobodanka and Savić, Miroslav and Cekić, Nebojša and Milić, J. and Vuleta, G",
year = "2004",
abstract = "It is known that, depending on the concentration, treatment with urea could improve skin barrier function, despite its penetration-enhancing properties. This controversial skin effect of urea has been explored systematically in this study in terms of the effect of vehicle on the performance of urea. In the first part, a series of four semi-solid emulsions with 5% (w/w) urea, varying in the type of emulsion, nature of emulsifier and polarity of oil ingredients, have been evaluated with regard to their skin hydrating and transepidermal water loss (TEWL)-modifying properties. Placebo samples were tested alongside the urea-containing ones. Two best performing moisturisers from the above were chosen for the second part of the study, in which sodium lauryl sulphate (SLS)-irritated skin was treated with both placebo and urea-containing samples. In addition to TEWL and skin hydration level, the erythema index (EI) was measured before, during and after the treatment. The results have shown that barrier-improving and hydrating abilities of urea are bi-directional and dependent on both the type of vehicle used for its delivery and the state of skin.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Vehicle-controlled effect of urea on normal and SLS-irritated skin",
volume = "271",
number = "1-2",
pages = "269-280",
doi = "10.1016/j.ijpharm.2003.11.033"
}

Savić, S., Tamburić, S., Savić, M., Cekić, N., Milić, J.,& Vuleta, G.. (2004). Vehicle-controlled effect of urea on normal and SLS-irritated skin. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 271(1-2), 269-280.
https://doi.org/10.1016/j.ijpharm.2003.11.033

Savić S, Tamburić S, Savić M, Cekić N, Milić J, Vuleta G. Vehicle-controlled effect of urea on normal and SLS-irritated skin. in International Journal of Pharmaceutics. 2004;271(1-2):269-280.
doi:10.1016/j.ijpharm.2003.11.033 .

Savić, Snežana, Tamburić, Slobodanka, Savić, Miroslav, Cekić, Nebojša, Milić, J., Vuleta, G, "Vehicle-controlled effect of urea on normal and SLS-irritated skin" in International Journal of Pharmaceutics, 271, no. 1-2 (2004):269-280,
https://doi.org/10.1016/j.ijpharm.2003.11.033 . .