TY  - JOUR
AU  - Zengin, Gökhan
AU  - Mollica, Adriano
AU  - Arsenijević, Jelena
AU  - Pavlić, Branimir
AU  - Zeković, Zoran
AU  - Sinan, Kouadio Ibrahime
AU  - Yan, Linlin
AU  - Cvetanović-Kljakić, Aleksandra
AU  - Ražić, Slavica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4418
AB  - Bearing in mind the centuries-old traditional use of chamomile, but also the increasing demand for its products in modern industry, oriented toward sustainable development, there are increasing efforts for the efficient extraction of high-value compounds of this plant, as well as obtaining its products with added value. With that goal, conventional and contemporary separation techniques were applied in this work. Both hydrodistillation processes (HD), conducted in a traditional manner and coupled with microwave irradiation (MWHD), were used for essential oil isolation. In parallel with those procedures, chamomile lipophilic extracts were obtained by Soxhlet extraction applying organic solvents and using supercritical fluid extraction as a greener approach. The obtained extracts and essential oils were characterized in terms of chemical composition (GC analysis, contents of total phenolics and flavonoids) and biological potential. GC analysis revealed that oxygenated sesquiterpenes and non-terpene compounds were the dominant compounds. α-Bisabolol oxide A (29.71–34.41%) and α-bisabolol oxide B (21.06–25.83%) were the most abundant individual components in samples obtained by distillation while in supercritical and Soxhlet extracts, major compounds were α-bisabolol oxide A and pentacosane. The biological potential of essential oils and extracts was tested by applying a set of analyzes to estimate the inhibition of biologically important enzymes (amylase, glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase) and antioxidant capacity (DPPH, ABTS, CUPRAC, FRAP, chelating and total antioxidant capacity). The results suggested essential oils as better antioxidants, while the extracts were proven to be better inhibitors of the tested enzymes. Principal Component Analysis was conducted using the experimental results of the composition of extracts and EOs of chamomile obtained by different separation techniques, showing clear discrimination between methods applied in correlation with the chemical profile. Molecular docking was applied for the identification of the main active principles present in the essential oil, among which α-bisabolol-oxide B (cp3) showed a higher affinity for tyrosinase.
PB  - MDPI
T2  - Separations
T1  - A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity
VL  - 10
IS  - 1
DO  - 10.3390/separations10010018
ER  - 

@article{
author = "Zengin, Gökhan and Mollica, Adriano and Arsenijević, Jelena and Pavlić, Branimir and Zeković, Zoran and Sinan, Kouadio Ibrahime and Yan, Linlin and Cvetanović-Kljakić, Aleksandra and Ražić, Slavica",
year = "2023",
abstract = "Bearing in mind the centuries-old traditional use of chamomile, but also the increasing demand for its products in modern industry, oriented toward sustainable development, there are increasing efforts for the efficient extraction of high-value compounds of this plant, as well as obtaining its products with added value. With that goal, conventional and contemporary separation techniques were applied in this work. Both hydrodistillation processes (HD), conducted in a traditional manner and coupled with microwave irradiation (MWHD), were used for essential oil isolation. In parallel with those procedures, chamomile lipophilic extracts were obtained by Soxhlet extraction applying organic solvents and using supercritical fluid extraction as a greener approach. The obtained extracts and essential oils were characterized in terms of chemical composition (GC analysis, contents of total phenolics and flavonoids) and biological potential. GC analysis revealed that oxygenated sesquiterpenes and non-terpene compounds were the dominant compounds. α-Bisabolol oxide A (29.71–34.41%) and α-bisabolol oxide B (21.06–25.83%) were the most abundant individual components in samples obtained by distillation while in supercritical and Soxhlet extracts, major compounds were α-bisabolol oxide A and pentacosane. The biological potential of essential oils and extracts was tested by applying a set of analyzes to estimate the inhibition of biologically important enzymes (amylase, glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase) and antioxidant capacity (DPPH, ABTS, CUPRAC, FRAP, chelating and total antioxidant capacity). The results suggested essential oils as better antioxidants, while the extracts were proven to be better inhibitors of the tested enzymes. Principal Component Analysis was conducted using the experimental results of the composition of extracts and EOs of chamomile obtained by different separation techniques, showing clear discrimination between methods applied in correlation with the chemical profile. Molecular docking was applied for the identification of the main active principles present in the essential oil, among which α-bisabolol-oxide B (cp3) showed a higher affinity for tyrosinase.",
publisher = "MDPI",
journal = "Separations",
title = "A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity",
volume = "10",
number = "1",
doi = "10.3390/separations10010018"
}

Zengin, G., Mollica, A., Arsenijević, J., Pavlić, B., Zeković, Z., Sinan, K. I., Yan, L., Cvetanović-Kljakić, A.,& Ražić, S.. (2023). A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity. in Separations
MDPI., 10(1).
https://doi.org/10.3390/separations10010018

Zengin G, Mollica A, Arsenijević J, Pavlić B, Zeković Z, Sinan KI, Yan L, Cvetanović-Kljakić A, Ražić S. A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity. in Separations. 2023;10(1).
doi:10.3390/separations10010018 .

Zengin, Gökhan, Mollica, Adriano, Arsenijević, Jelena, Pavlić, Branimir, Zeković, Zoran, Sinan, Kouadio Ibrahime, Yan, Linlin, Cvetanović-Kljakić, Aleksandra, Ražić, Slavica, "A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity" in Separations, 10, no. 1 (2023),
https://doi.org/10.3390/separations10010018 . .

TY  - JOUR
AU  - Semenova, Irina
AU  - Bryskina, Diana
AU  - Cvetanović Kljakić, Aleksandra
AU  - Ražić, Slavica
AU  - Ananiev, Vasiliy
AU  - Rodin, Igor
AU  - Shpigun, Oleg
AU  - Stavrianidi, Andrey
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3133
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4115
AB  - Introduction: Limited availability of individual standards is a bottleneck for quality control of functional foods and natural medicines. The use of standard mixtures or secondary standards is a possible alternative in this case. Earlier, an approach known as standardised reference extract (RE) strategy was introduced for HPLC-UV analysis of different plant materials; however, its application in HPLC-MS analysis has not been investigated. Objective: To establish an HPLC-MS-based RE method for determination of ginsenoside content in ginseng infusions using commercially available extract refer- ence material of Panax quinquefolius L. Results: The developed HPLC-MS method was validated as precise (1.1%–9.4% intra-day variation; 1.6%–12.8% inter-day variation) and highly sensitive [limit of detection (LOD): 1–40 ng/mL; limit of quantification (LOQ): 4–120 ng/mL]. The sta- bility of samples was satisfactory (5.7%–16.3%). The RE quantification method was compared with the external standard method, and the obtained difference was not significant, mostly in the range of 5%–10%. Matrix effects for the diluted samples of RE and ginseng infusions, determined via the standard addition method, were in the range of 85%–115% and 80%–126%, respectively, and were also positively corre- lated with the ginsenoside concentration. Eleven batches of ginseng infusions from different manufacturers were analysed using the established method. Conclusion: The method for HPLC-MS-based ginsenoside quantification using RE as a secondary standard was established for the first time. The results of this study dem- onstrate that the application of the standardised RE strategy in HPLC-MS can mini- mise the matrix effect-related error in addition to the cost-effective quality control of herbal products, foods, and traditional medicines.
PB  - John Wiley and Sons Ltd
T2  - Phytochemical Analysis
T1  - An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection
VL  - 33
IS  - 6
SP  - 838
EP  - 850
DO  - 10.1002/pca.3133
ER  - 

@article{
author = "Semenova, Irina and Bryskina, Diana and Cvetanović Kljakić, Aleksandra and Ražić, Slavica and Ananiev, Vasiliy and Rodin, Igor and Shpigun, Oleg and Stavrianidi, Andrey",
year = "2022",
abstract = "Introduction: Limited availability of individual standards is a bottleneck for quality control of functional foods and natural medicines. The use of standard mixtures or secondary standards is a possible alternative in this case. Earlier, an approach known as standardised reference extract (RE) strategy was introduced for HPLC-UV analysis of different plant materials; however, its application in HPLC-MS analysis has not been investigated. Objective: To establish an HPLC-MS-based RE method for determination of ginsenoside content in ginseng infusions using commercially available extract refer- ence material of Panax quinquefolius L. Results: The developed HPLC-MS method was validated as precise (1.1%–9.4% intra-day variation; 1.6%–12.8% inter-day variation) and highly sensitive [limit of detection (LOD): 1–40 ng/mL; limit of quantification (LOQ): 4–120 ng/mL]. The sta- bility of samples was satisfactory (5.7%–16.3%). The RE quantification method was compared with the external standard method, and the obtained difference was not significant, mostly in the range of 5%–10%. Matrix effects for the diluted samples of RE and ginseng infusions, determined via the standard addition method, were in the range of 85%–115% and 80%–126%, respectively, and were also positively corre- lated with the ginsenoside concentration. Eleven batches of ginseng infusions from different manufacturers were analysed using the established method. Conclusion: The method for HPLC-MS-based ginsenoside quantification using RE as a secondary standard was established for the first time. The results of this study dem- onstrate that the application of the standardised RE strategy in HPLC-MS can mini- mise the matrix effect-related error in addition to the cost-effective quality control of herbal products, foods, and traditional medicines.",
publisher = "John Wiley and Sons Ltd",
journal = "Phytochemical Analysis",
title = "An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection",
volume = "33",
number = "6",
pages = "838-850",
doi = "10.1002/pca.3133"
}

Semenova, I., Bryskina, D., Cvetanović Kljakić, A., Ražić, S., Ananiev, V., Rodin, I., Shpigun, O.,& Stavrianidi, A.. (2022). An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection. in Phytochemical Analysis
John Wiley and Sons Ltd., 33(6), 838-850.
https://doi.org/10.1002/pca.3133

Semenova I, Bryskina D, Cvetanović Kljakić A, Ražić S, Ananiev V, Rodin I, Shpigun O, Stavrianidi A. An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection. in Phytochemical Analysis. 2022;33(6):838-850.
doi:10.1002/pca.3133 .

Semenova, Irina, Bryskina, Diana, Cvetanović Kljakić, Aleksandra, Ražić, Slavica, Ananiev, Vasiliy, Rodin, Igor, Shpigun, Oleg, Stavrianidi, Andrey, "An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection" in Phytochemical Analysis, 33, no. 6 (2022):838-850,
https://doi.org/10.1002/pca.3133 . .