TY  - JOUR
AU  - Davidović, Vesna
AU  - Lazarević, Miodrag
AU  - Joksimović-Todorović, Mirjana
AU  - Maksimović, Zoran
AU  - Jovanović, Milan
AU  - Miljas, Niko
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1439
AB  - This paper presents the results of investigations of parameter values of the red blood cell count in Wistar rats, after intramuscular application of rhizome and root extract of Helleborus odorus W. et K (HE). The trial was conducted in experimental conditions on 49 Wistar rats divided into 7 groups with 7 animals per unit (control and six experimental groups). The control group was treated by an intramuscular injection of sterile saline (0.25 ml/100 g BM). For the purpose of determing the duration of the HE effects, the animals were injected with a dose of 10 mg/100 g BMand blood was collected by cardial taps after 24, 48 and 72 hrs. Moreover, we investigated the influence of elevated temperatures on the noted HE extract effects, 24 hrs after the application of the inactivated (56oC 30 min) or denaturized (100oC 5 min) extract, as well as the influence of dialyzed HE. The obtained results of haematological analyses have pointed out the weak hemolyses activity of the extract. In all experimental groups, the erythrocyte count was decreased, but remained within reference values. Haemoglobin concentration and haematocrit values in the experimental and the control groups of rats were decreased in comparison to the physiological values, while the applied extract did not significantly affect the examined parameters. .
AB  - U ovom radu su prikazani rezultati ispitivanja vrednosti parametara crvene krvne slike pacova soja Wistar, nakon intramuskularne aplikacije ekstrakta rizoma i korena Helleborus odorus W. et K. Ogled je izveden u eksperimentalnim uslovima na 49 pacova soja Wistar, podeljenih u 7 grupa sa po 7 jedinki (jedna kontrolna i šest oglednih grupa). Kontrolnoj grupi pacova je intramuskularno aplikovan fiziološki rastvor (0,25 ml/100 g t.m.). U cilju određivanja dužine trajanja efekata ekstrakta, pacovima je aplikovan tečni ekstrakt podzemnih organa kukureka u koncentraciji od 10 mg/100 g t.m., a krv je uzorkovana kardijalnom punkcijom nakon 24h, 48h i 72h. Ispitivan je i uticaj ekstrakta izlaganog povišenoj temperaturi na uočene efekte, 24h posle aplikacije inaktivisanog (56oC 30 min) ili denaturisanog ekstrakta (100oC 5 min), kao i efekat dijalizata ekstrakta. Dobijeni rezultati hematoloških analiza su ukazali na slabu hemolitičku aktivnost ekstrakta. Kod svih oglednih grupa, prosečan broj eritrocita bio je smanjen, ali se održao u granicama referentnih vrednosti. Zabeležene koncentracije hemoglobina i hematokrita u krvi pacova kontrolne i oglednih grupa bile su niže u odnosu na fiziološke vrednosti, pri čemu korišćeni ekstrakt nije značajno uticao na ispitivane parametre.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats
T1  - Uticaj ekstrakta podzemnih organa Helleborus odorus w. et K. na crvenu krvnu sliku Wistar pacova
VL  - 64
IS  - 3-4
SP  - 219
EP  - 229
DO  - 10.2298/VETGL1004219D
ER  - 

@article{
author = "Davidović, Vesna and Lazarević, Miodrag and Joksimović-Todorović, Mirjana and Maksimović, Zoran and Jovanović, Milan and Miljas, Niko",
year = "2010",
abstract = "This paper presents the results of investigations of parameter values of the red blood cell count in Wistar rats, after intramuscular application of rhizome and root extract of Helleborus odorus W. et K (HE). The trial was conducted in experimental conditions on 49 Wistar rats divided into 7 groups with 7 animals per unit (control and six experimental groups). The control group was treated by an intramuscular injection of sterile saline (0.25 ml/100 g BM). For the purpose of determing the duration of the HE effects, the animals were injected with a dose of 10 mg/100 g BMand blood was collected by cardial taps after 24, 48 and 72 hrs. Moreover, we investigated the influence of elevated temperatures on the noted HE extract effects, 24 hrs after the application of the inactivated (56oC 30 min) or denaturized (100oC 5 min) extract, as well as the influence of dialyzed HE. The obtained results of haematological analyses have pointed out the weak hemolyses activity of the extract. In all experimental groups, the erythrocyte count was decreased, but remained within reference values. Haemoglobin concentration and haematocrit values in the experimental and the control groups of rats were decreased in comparison to the physiological values, while the applied extract did not significantly affect the examined parameters. ., U ovom radu su prikazani rezultati ispitivanja vrednosti parametara crvene krvne slike pacova soja Wistar, nakon intramuskularne aplikacije ekstrakta rizoma i korena Helleborus odorus W. et K. Ogled je izveden u eksperimentalnim uslovima na 49 pacova soja Wistar, podeljenih u 7 grupa sa po 7 jedinki (jedna kontrolna i šest oglednih grupa). Kontrolnoj grupi pacova je intramuskularno aplikovan fiziološki rastvor (0,25 ml/100 g t.m.). U cilju određivanja dužine trajanja efekata ekstrakta, pacovima je aplikovan tečni ekstrakt podzemnih organa kukureka u koncentraciji od 10 mg/100 g t.m., a krv je uzorkovana kardijalnom punkcijom nakon 24h, 48h i 72h. Ispitivan je i uticaj ekstrakta izlaganog povišenoj temperaturi na uočene efekte, 24h posle aplikacije inaktivisanog (56oC 30 min) ili denaturisanog ekstrakta (100oC 5 min), kao i efekat dijalizata ekstrakta. Dobijeni rezultati hematoloških analiza su ukazali na slabu hemolitičku aktivnost ekstrakta. Kod svih oglednih grupa, prosečan broj eritrocita bio je smanjen, ali se održao u granicama referentnih vrednosti. Zabeležene koncentracije hemoglobina i hematokrita u krvi pacova kontrolne i oglednih grupa bile su niže u odnosu na fiziološke vrednosti, pri čemu korišćeni ekstrakt nije značajno uticao na ispitivane parametre.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats, Uticaj ekstrakta podzemnih organa Helleborus odorus w. et K. na crvenu krvnu sliku Wistar pacova",
volume = "64",
number = "3-4",
pages = "219-229",
doi = "10.2298/VETGL1004219D"
}

Davidović, V., Lazarević, M., Joksimović-Todorović, M., Maksimović, Z., Jovanović, M.,& Miljas, N.. (2010). Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3-4), 219-229.
https://doi.org/10.2298/VETGL1004219D

Davidović V, Lazarević M, Joksimović-Todorović M, Maksimović Z, Jovanović M, Miljas N. Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats. in Veterinarski glasnik. 2010;64(3-4):219-229.
doi:10.2298/VETGL1004219D .

Davidović, Vesna, Lazarević, Miodrag, Joksimović-Todorović, Mirjana, Maksimović, Zoran, Jovanović, Milan, Miljas, Niko, "Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats" in Veterinarski glasnik, 64, no. 3-4 (2010):219-229,
https://doi.org/10.2298/VETGL1004219D . .

TY  - JOUR
AU  - Davidović, Vesna
AU  - Lazarević, M.
AU  - Joksimović-Todorović, Mirjana
AU  - Maksimović, Zoran
AU  - Jovanović, M.
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1441
AB  - The objective of this research was to study the effects that the extract of rhizome and root of Helleborus odorus W. et K. (Ranunculaceae) can have on modifications in the parameter values of white blood cells count and degree of phagocytosis by peritoneal macrophages and neutrophil granulocytes in Wistar rats. The trial was conducted on 28 rats divided into 4 groups with 7 animals in each group. To the control group of rats sterile physiological solution in the quantity of 0.25 mL/100 g BW was applied intramuscularly. For the purpose of monitoring the effect of the extract of rhizome and root of hellebore (HE) during a time period, the HE was applied intramuscularly to rats in a dose of 10 mg/100 g BW, while the blood samples for analysis were taken after 24h, 48h and 72h. The consequence of intramuscular application of HE was an increased count of total leukocytes in all trial groups, the most expressed leukocytosis being registered 24h after application of HE. Statistically significant higher value in the count and percent of neutrophil granulocytes in the blood was recorded 24h after treatment in relation to the control and two other trial groups (p lt 0.001), among which a statistical significance was not established. The extract of hellebore rhizome and root has led to lymphopenia, resulting in the increase of the neutrophil/limphocyte index in the trial groups 24h and 48h after treatment. The application of HE had no significant effect on the count of monocytes in treated animals. The applied extract has caused a significant increase in the degree of phagocytosis by residing peritoneal macrophages and neutrophil granulocytes in blood.
AB  - Cilj ovog rada je bio da se ispita uticaj ekstrakta rizoma i korena H. odorus W. et K. na promenu vrednosti parametara bele krvne slike i stepen fagocitoze od strane peritonealnih makrofaga i neutrofilnih granulocita kod pacova soja Wistar. Ogled je izveden na 28 pacova podeljenih u 4 grupe po 7 jedinki. Kontrolnoj grupi pacova je intramuskularno aplikovan sterilan fiziološki rastvor u količini od 0,25 ml/100 g TM. U cilju praćenja efekta ekstrakta rizoma i korena kukureka (EK) u toku vremena, pacovima je intramuskularno aplikovan EK u dozi od 10mg/100g TM, a krv za analizu je uzimana posle 24h, 48h i 72h. Intramuskularna aplikacija EK imala je za posledicu povećanje broja ukupnih leukocita u svim oglednim grupama, pri čemu je najizraženija leukocitoza registrovana 24h nakon aplikovanja EK. Statistički značajno veća vrednost broja i procenta neutrofilnih granulocita u krvi zabeležena je 24h posle tretmana u odnosu na kontrolnu i ostale dve ogledne grupe (p lt 0,001), između kojih nije utvrđena statistička značajnost. Ekstrakt rizoma i korena kukureka doveo je do nastanka limfopenije, što je imalo za posledicu povećanje neutrofilno/limfocitnog indeksa u oglednim grupama 24h i 48h nakon tretmana. Aplikacija EK nije značajno uticala na broj monocita kod tretiranih životinja. Upotrebljeni ekstrakt doveo je do značajnog povećanja stepena fagocitoze od strane rezidentnih peritonealnih makrofaga i neutrofilnih granulocita krvi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats
T1  - Uticaj ekstrakta rizoma i korena kukureka (Helleborus odorus W. et K.) na parametre bele krvne slike i stepen fagocitoze kod wistar pacova
VL  - 60
IS  - 5-6
SP  - 605
EP  - 618
DO  - 10.2298/AVB1006605D
ER  - 

@article{
author = "Davidović, Vesna and Lazarević, M. and Joksimović-Todorović, Mirjana and Maksimović, Zoran and Jovanović, M.",
year = "2010",
abstract = "The objective of this research was to study the effects that the extract of rhizome and root of Helleborus odorus W. et K. (Ranunculaceae) can have on modifications in the parameter values of white blood cells count and degree of phagocytosis by peritoneal macrophages and neutrophil granulocytes in Wistar rats. The trial was conducted on 28 rats divided into 4 groups with 7 animals in each group. To the control group of rats sterile physiological solution in the quantity of 0.25 mL/100 g BW was applied intramuscularly. For the purpose of monitoring the effect of the extract of rhizome and root of hellebore (HE) during a time period, the HE was applied intramuscularly to rats in a dose of 10 mg/100 g BW, while the blood samples for analysis were taken after 24h, 48h and 72h. The consequence of intramuscular application of HE was an increased count of total leukocytes in all trial groups, the most expressed leukocytosis being registered 24h after application of HE. Statistically significant higher value in the count and percent of neutrophil granulocytes in the blood was recorded 24h after treatment in relation to the control and two other trial groups (p lt 0.001), among which a statistical significance was not established. The extract of hellebore rhizome and root has led to lymphopenia, resulting in the increase of the neutrophil/limphocyte index in the trial groups 24h and 48h after treatment. The application of HE had no significant effect on the count of monocytes in treated animals. The applied extract has caused a significant increase in the degree of phagocytosis by residing peritoneal macrophages and neutrophil granulocytes in blood., Cilj ovog rada je bio da se ispita uticaj ekstrakta rizoma i korena H. odorus W. et K. na promenu vrednosti parametara bele krvne slike i stepen fagocitoze od strane peritonealnih makrofaga i neutrofilnih granulocita kod pacova soja Wistar. Ogled je izveden na 28 pacova podeljenih u 4 grupe po 7 jedinki. Kontrolnoj grupi pacova je intramuskularno aplikovan sterilan fiziološki rastvor u količini od 0,25 ml/100 g TM. U cilju praćenja efekta ekstrakta rizoma i korena kukureka (EK) u toku vremena, pacovima je intramuskularno aplikovan EK u dozi od 10mg/100g TM, a krv za analizu je uzimana posle 24h, 48h i 72h. Intramuskularna aplikacija EK imala je za posledicu povećanje broja ukupnih leukocita u svim oglednim grupama, pri čemu je najizraženija leukocitoza registrovana 24h nakon aplikovanja EK. Statistički značajno veća vrednost broja i procenta neutrofilnih granulocita u krvi zabeležena je 24h posle tretmana u odnosu na kontrolnu i ostale dve ogledne grupe (p lt 0,001), između kojih nije utvrđena statistička značajnost. Ekstrakt rizoma i korena kukureka doveo je do nastanka limfopenije, što je imalo za posledicu povećanje neutrofilno/limfocitnog indeksa u oglednim grupama 24h i 48h nakon tretmana. Aplikacija EK nije značajno uticala na broj monocita kod tretiranih životinja. Upotrebljeni ekstrakt doveo je do značajnog povećanja stepena fagocitoze od strane rezidentnih peritonealnih makrofaga i neutrofilnih granulocita krvi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats, Uticaj ekstrakta rizoma i korena kukureka (Helleborus odorus W. et K.) na parametre bele krvne slike i stepen fagocitoze kod wistar pacova",
volume = "60",
number = "5-6",
pages = "605-618",
doi = "10.2298/AVB1006605D"
}

Davidović, V., Lazarević, M., Joksimović-Todorović, M., Maksimović, Z.,& Jovanović, M.. (2010). The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 605-618.
https://doi.org/10.2298/AVB1006605D

Davidović V, Lazarević M, Joksimović-Todorović M, Maksimović Z, Jovanović M. The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats. in Acta veterinaria. 2010;60(5-6):605-618.
doi:10.2298/AVB1006605D .

Davidović, Vesna, Lazarević, M., Joksimović-Todorović, Mirjana, Maksimović, Zoran, Jovanović, M., "The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats" in Acta veterinaria, 60, no. 5-6 (2010):605-618,
https://doi.org/10.2298/AVB1006605D . .

TY  - JOUR
AU  - Davidović, Vesna
AU  - Lazarević, Miodrag
AU  - Joksimović-Todorović, Mirjana
AU  - Maksimović, Zoran
AU  - Jovanović, Milan
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1131
AB  - In the prevention and treatment of milder forms of diseases, chronic diseases and recurrent infections in farm animals the application of individual or combined medicinal herb preparations has long been used. The application of some herbal preparations can initiate the function of the effectors defensive cells and potentiate their defensive capacities. On the other hand, some immuno-suppresive phytopreparations are used in order to reduce or prevent undesired activities of immune system (allergic reactions and autoimmune disease). By the regulations of the European Union from 2006 the use of antibiotics as growth stimulators in livestock production was forbidden so aromatic herbs and their extracts have recently been more and more used for that purposes. .
AB  - U prevenciji i lečenju blažih oblika bolesti, bolesti hroničnog toka i rekurentnih infekcija domaćih životinja, već dugo se primenjuju pojedinačna ili kombinovana biljna lekovita sredstva. Primena pojedinih biljnih preparata podstiče funkcije efektorskih odbrambenih ćelija organizma i potencira njegove odbrambene sposobnosti. Sa druge strane, neki imunosupresivni fitopreparati se upotrebljavaju u cilju smanjenja ili sprečavanja neželjenih aktivnosti imunog sistema (alergijskih reakcija i autoimunih bolesti). Propisima Evropske Unije zabranjena je od 2006. godine upotreba antibiotika kao stimulatora rasta u stočarskoj proizvodnji, pa se poslednjih godina u te svrhe sve više koriste aromatične biljke i njihovi ekstrakti. .
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - The application of Helleborus L. and other herbs in prophylaxis and therapy in animal diseases
T1  - Primena Helleborus L. i drugog bilja u profilaksi i terapiji bolesti životinja
IS  - 28
SP  - 63
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1646
ER  - 

@article{
author = "Davidović, Vesna and Lazarević, Miodrag and Joksimović-Todorović, Mirjana and Maksimović, Zoran and Jovanović, Milan",
year = "2008",
abstract = "In the prevention and treatment of milder forms of diseases, chronic diseases and recurrent infections in farm animals the application of individual or combined medicinal herb preparations has long been used. The application of some herbal preparations can initiate the function of the effectors defensive cells and potentiate their defensive capacities. On the other hand, some immuno-suppresive phytopreparations are used in order to reduce or prevent undesired activities of immune system (allergic reactions and autoimmune disease). By the regulations of the European Union from 2006 the use of antibiotics as growth stimulators in livestock production was forbidden so aromatic herbs and their extracts have recently been more and more used for that purposes. ., U prevenciji i lečenju blažih oblika bolesti, bolesti hroničnog toka i rekurentnih infekcija domaćih životinja, već dugo se primenjuju pojedinačna ili kombinovana biljna lekovita sredstva. Primena pojedinih biljnih preparata podstiče funkcije efektorskih odbrambenih ćelija organizma i potencira njegove odbrambene sposobnosti. Sa druge strane, neki imunosupresivni fitopreparati se upotrebljavaju u cilju smanjenja ili sprečavanja neželjenih aktivnosti imunog sistema (alergijskih reakcija i autoimunih bolesti). Propisima Evropske Unije zabranjena je od 2006. godine upotreba antibiotika kao stimulatora rasta u stočarskoj proizvodnji, pa se poslednjih godina u te svrhe sve više koriste aromatične biljke i njihovi ekstrakti. .",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "The application of Helleborus L. and other herbs in prophylaxis and therapy in animal diseases, Primena Helleborus L. i drugog bilja u profilaksi i terapiji bolesti životinja",
number = "28",
pages = "63-69",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1646"
}

Davidović, V., Lazarević, M., Joksimović-Todorović, M., Maksimović, Z.,& Jovanović, M.. (2008). The application of Helleborus L. and other herbs in prophylaxis and therapy in animal diseases. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(28), 63-69.
https://hdl.handle.net/21.15107/rcub_agrospace_1646

Davidović V, Lazarević M, Joksimović-Todorović M, Maksimović Z, Jovanović M. The application of Helleborus L. and other herbs in prophylaxis and therapy in animal diseases. in Lekovite sirovine. 2008;(28):63-69.
https://hdl.handle.net/21.15107/rcub_agrospace_1646 .

Davidović, Vesna, Lazarević, Miodrag, Joksimović-Todorović, Mirjana, Maksimović, Zoran, Jovanović, Milan, "The application of Helleborus L. and other herbs in prophylaxis and therapy in animal diseases" in Lekovite sirovine, no. 28 (2008):63-69,
https://hdl.handle.net/21.15107/rcub_agrospace_1646 .