TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Branković, Ivana
AU  - Lađarević, Jelena
AU  - Pavun, Leposava
AU  - Kostić, Mirjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5060
AB  - The raw jute fabric was oxidized with 0.2% or 0.4% NaIO4 for 60 or 120 min to obtain valuable adsorbents for the
textile dye Congo Red (CR). Batch adsorption experiments were carried out as a function of solution pH, contact
time, initial CR concentration, and temperature. At an initial CR concentration of 25 mg/L, the raw jute possessed the
lowest adsorption due to the lowest availability and content of carboxyl and aldehyde groups capable of binding dye. The
fabrics oxidized for 60 min with 0.2% NaIO4 and 120 min with 0.4% NaIO4 reached equilibrium adsorption after 240 and
330 min, respectively. The adsorption of CR onto studied fabrics followed the pseudo-second-order model indicating
that the chemisorption process is primarily represented. Within the studied range of CR concentrations (25–100 mg/L),
its adsorption onto fabric oxidized for 120 min with 0.4% NaIO4 obeys the Langmuir isotherm model (i.e. adsorption
occurs at specific, energetically equivalent adsorbent sites with monolayer coverage of CR over a homogeneous surface),
wherein the calculated maximal adsorption capacity is 12.863 mg/g. A thermodynamic study revealed the spontaneous
and endothermic character of CR adsorption onto jute fabric. Based on the recorded ATR-FTIR spectra before and after
CR adsorption, it is suggested that repulsion, hydrogen bonds, and π-π stacking interactions are involved in the binding
of CR onto jute fabrics.
PB  - Sage Journals
T2  - Journal of Engineered Fibers and Fabrics
T1  - Oxidized jute as a valuable adsorbent for Congo Red from an aqueous solution
VL  - 17
DO  - 10.1177/15589250221101380
ER  - 

@article{
author = "Ivanovska, Aleksandra and Branković, Ivana and Lađarević, Jelena and Pavun, Leposava and Kostić, Mirjana",
year = "2022",
abstract = "The raw jute fabric was oxidized with 0.2% or 0.4% NaIO4 for 60 or 120 min to obtain valuable adsorbents for the
textile dye Congo Red (CR). Batch adsorption experiments were carried out as a function of solution pH, contact
time, initial CR concentration, and temperature. At an initial CR concentration of 25 mg/L, the raw jute possessed the
lowest adsorption due to the lowest availability and content of carboxyl and aldehyde groups capable of binding dye. The
fabrics oxidized for 60 min with 0.2% NaIO4 and 120 min with 0.4% NaIO4 reached equilibrium adsorption after 240 and
330 min, respectively. The adsorption of CR onto studied fabrics followed the pseudo-second-order model indicating
that the chemisorption process is primarily represented. Within the studied range of CR concentrations (25–100 mg/L),
its adsorption onto fabric oxidized for 120 min with 0.4% NaIO4 obeys the Langmuir isotherm model (i.e. adsorption
occurs at specific, energetically equivalent adsorbent sites with monolayer coverage of CR over a homogeneous surface),
wherein the calculated maximal adsorption capacity is 12.863 mg/g. A thermodynamic study revealed the spontaneous
and endothermic character of CR adsorption onto jute fabric. Based on the recorded ATR-FTIR spectra before and after
CR adsorption, it is suggested that repulsion, hydrogen bonds, and π-π stacking interactions are involved in the binding
of CR onto jute fabrics.",
publisher = "Sage Journals",
journal = "Journal of Engineered Fibers and Fabrics",
title = "Oxidized jute as a valuable adsorbent for Congo Red from an aqueous solution",
volume = "17",
doi = "10.1177/15589250221101380"
}

Ivanovska, A., Branković, I., Lađarević, J., Pavun, L.,& Kostić, M.. (2022). Oxidized jute as a valuable adsorbent for Congo Red from an aqueous solution. in Journal of Engineered Fibers and Fabrics
Sage Journals., 17.
https://doi.org/10.1177/15589250221101380

Ivanovska A, Branković I, Lađarević J, Pavun L, Kostić M. Oxidized jute as a valuable adsorbent for Congo Red from an aqueous solution. in Journal of Engineered Fibers and Fabrics. 2022;17.
doi:10.1177/15589250221101380 .

Ivanovska, Aleksandra, Branković, Ivana, Lađarević, Jelena, Pavun, Leposava, Kostić, Mirjana, "Oxidized jute as a valuable adsorbent for Congo Red from an aqueous solution" in Journal of Engineered Fibers and Fabrics, 17 (2022),
https://doi.org/10.1177/15589250221101380 . .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Pavun, Leposava
AU  - Dojčinović, Biljana
AU  - Kostić, Mirjana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3955
AB  - Raw jute fabric was chemically modified with 1 % NaOH for 30 min,  17.5 % NaOH for 5 min or with 0.7 % NaClO2 for 30 and 60 min to study the  influence of changes in the chemical composition of the fabrics and quantity of  carboxyl groups on the biosorption capacity of the fabrics for nickel ions. The  effects of contact time and initial concentration of nickel ions on the biosorp- tion capacity of the fabrics were also investigated. The obtained results rev- ealed that the biosorption of nickel ions can be explained by the pseudo-sec- ond-order kinetic model, while the experimental isotherm data fit better with  the Langmuir model. The calculated ratios between maximal biosorption cap- acity (6.30–12.06 mg g-1) of the jute fabrics and carboxyl group quantity indi- cated that approximately half of the carboxyl groups of the fabrics’ would be  involved in binding nickel ions during biosorption. Therefore, the quantity of  carboxyl groups can be used to predict the maximal biosorption capacity of jute  fabrics toward nickel ions. In the case of oxidized jute fabrics, the lignin rem- oval, and consequently increased content of cellulose and hemicelluloses, also  contributed to a higher biosorption capacity.
AB  - Сирова тканина од јуте је хемијски модификована 1 % NaOH у току 30 min, 17,5 %  NaOH у току 5 min или 0,7 % NaClO2 у току 30 и 60 min у циљу испитивања утицаја промена хемијског састава и садржаја карбоксилних група на биосорпциони капацитет тканина  према  јонима  никла.  Такође,  испитиван  је  и  утицај  времена  контакта  тканина  са  раствором  NiSO4  и  почетне  концентрације  раствора  никла  на  биосорпциони  капацитет  тканина од јуте. Добијени резултати указују на то да се процес биосорпције никла може  описати  кинетичким  моделом  псеудо-другог  реда,  а  равнотежне  вредности  добијене  за  сорпцију никла се боље слажу са Langmuir адсорпционом изотермом. Израчунати однос  између  максималних  биосорпционих  капацитета  тканина  од  јуте  (6,30–12,06  mg  g-1)  и  садржаја  карбоксилних  група  указују  на  то  да  ће  приближно  половина  карбоксилних  група  учествовати  у  везивању  јона  никла  током  биосорпције.  Према  томе,  садржај  карбоксилних  група  се  може  користити  као  индикатор  максималног  биосорпционог  капацитета тканина од јуте према јонима никла. Код оксидисане тканине, уклањање лигнина  које  је  праћено  са  повећањем  садржаја  целулозе  и  хемицелулоза  је  такође  допринело  већем биосорпционом капацитету.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Kinetic and isotherm studies for the biosorption of nickel ions by jute fabrics
T1  - Биосорпција јона никла на тканинама од јуте: кинетичка и  изотермска испитивања
VL  - 86
IS  - 9
SP  - 885
EP  - 897
DO  - 10.2298/JSC210209030I
ER  - 

@article{
author = "Ivanovska, Aleksandra and Pavun, Leposava and Dojčinović, Biljana and Kostić, Mirjana",
year = "2021",
abstract = "Raw jute fabric was chemically modified with 1 % NaOH for 30 min,  17.5 % NaOH for 5 min or with 0.7 % NaClO2 for 30 and 60 min to study the  influence of changes in the chemical composition of the fabrics and quantity of  carboxyl groups on the biosorption capacity of the fabrics for nickel ions. The  effects of contact time and initial concentration of nickel ions on the biosorp- tion capacity of the fabrics were also investigated. The obtained results rev- ealed that the biosorption of nickel ions can be explained by the pseudo-sec- ond-order kinetic model, while the experimental isotherm data fit better with  the Langmuir model. The calculated ratios between maximal biosorption cap- acity (6.30–12.06 mg g-1) of the jute fabrics and carboxyl group quantity indi- cated that approximately half of the carboxyl groups of the fabrics’ would be  involved in binding nickel ions during biosorption. Therefore, the quantity of  carboxyl groups can be used to predict the maximal biosorption capacity of jute  fabrics toward nickel ions. In the case of oxidized jute fabrics, the lignin rem- oval, and consequently increased content of cellulose and hemicelluloses, also  contributed to a higher biosorption capacity., Сирова тканина од јуте је хемијски модификована 1 % NaOH у току 30 min, 17,5 %  NaOH у току 5 min или 0,7 % NaClO2 у току 30 и 60 min у циљу испитивања утицаја промена хемијског састава и садржаја карбоксилних група на биосорпциони капацитет тканина  према  јонима  никла.  Такође,  испитиван  је  и  утицај  времена  контакта  тканина  са  раствором  NiSO4  и  почетне  концентрације  раствора  никла  на  биосорпциони  капацитет  тканина од јуте. Добијени резултати указују на то да се процес биосорпције никла може  описати  кинетичким  моделом  псеудо-другог  реда,  а  равнотежне  вредности  добијене  за  сорпцију никла се боље слажу са Langmuir адсорпционом изотермом. Израчунати однос  између  максималних  биосорпционих  капацитета  тканина  од  јуте  (6,30–12,06  mg  g-1)  и  садржаја  карбоксилних  група  указују  на  то  да  ће  приближно  половина  карбоксилних  група  учествовати  у  везивању  јона  никла  током  биосорпције.  Према  томе,  садржај  карбоксилних  група  се  може  користити  као  индикатор  максималног  биосорпционог  капацитета тканина од јуте према јонима никла. Код оксидисане тканине, уклањање лигнина  које  је  праћено  са  повећањем  садржаја  целулозе  и  хемицелулоза  је  такође  допринело  већем биосорпционом капацитету.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Kinetic and isotherm studies for the biosorption of nickel ions by jute fabrics, Биосорпција јона никла на тканинама од јуте: кинетичка и  изотермска испитивања",
volume = "86",
number = "9",
pages = "885-897",
doi = "10.2298/JSC210209030I"
}

Ivanovska, A., Pavun, L., Dojčinović, B.,& Kostić, M.. (2021). Kinetic and isotherm studies for the biosorption of nickel ions by jute fabrics. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 86(9), 885-897.
https://doi.org/10.2298/JSC210209030I

Ivanovska A, Pavun L, Dojčinović B, Kostić M. Kinetic and isotherm studies for the biosorption of nickel ions by jute fabrics. in Journal of the Serbian Chemical Society. 2021;86(9):885-897.
doi:10.2298/JSC210209030I .

Ivanovska, Aleksandra, Pavun, Leposava, Dojčinović, Biljana, Kostić, Mirjana, "Kinetic and isotherm studies for the biosorption of nickel ions by jute fabrics" in Journal of the Serbian Chemical Society, 86, no. 9 (2021):885-897,
https://doi.org/10.2298/JSC210209030I . .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Lađarević, Jelena
AU  - Pavun, Leposava
AU  - Antonijević, Biljana
AU  - Cvijetić, Ilija
AU  - Mijin, Dušan
AU  - Kostić, Mirjana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3939
AB  - The objective of this investigation was to obtain jute fabrics with enhanced sorption properties (by using simple and cost-effective alkali and oxidative modifications) and a long life cycle. The applied alkali modifications lead to hemicellulose removal and decreased the fibers’ crystallinity, both contributing to enhanced fibers’ sorption properties, i.e., water retention power and degree of fibers’ swelling up to 49 % and 70 %, respectively. A connection between cellulose polymorphs’ (cellulose I and cellulose II) contents (determined by XRD), fibers’ surface morphology (verified by FESEM), fabrics’ crimp, and capillarity of jute fabrics modified with 17.5 % NaOH was established. During the oxidative modifications, significant changes in jute fibers’ chemical composition and structure (i.e., lignin removal and more homogeneous middle lamellae) occurred which further resulted in enhanced jute fabrics’ water retention power and capillarity as well as fibers’ swelling up to 80 %, 75 %, and 54 %, compared to the raw jute, respectively. In order to move towards a circular economy and to ensure the recycling and re-use of recycled fabrics, the jute fabrics with enhanced sorption properties were evaluated as biosorbents for anthraquinone dye C. I. Acid Blue 111. The obtained results revealed that the jute fabrics’ maximum biosorption capacities for this dye ranged from 12.94 to 18.97 mg/g, while the equilibrium adsorption data were highly consistent with the Langmuir isotherm model. Moreover, based on the predicted dye pKa values, the fabric zeta potential, content of carboxyl and aldehyde groups as well as hydrogen bond intensity (determined by ATR-FTIR), a possible mechanism of the dye biosorption onto jute fabric waste was proposed.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle
VL  - 171
DO  - 10.1016/j.indcrop.2021.113913
ER  - 

@article{
author = "Ivanovska, Aleksandra and Lađarević, Jelena and Pavun, Leposava and Antonijević, Biljana and Cvijetić, Ilija and Mijin, Dušan and Kostić, Mirjana",
year = "2021",
abstract = "The objective of this investigation was to obtain jute fabrics with enhanced sorption properties (by using simple and cost-effective alkali and oxidative modifications) and a long life cycle. The applied alkali modifications lead to hemicellulose removal and decreased the fibers’ crystallinity, both contributing to enhanced fibers’ sorption properties, i.e., water retention power and degree of fibers’ swelling up to 49 % and 70 %, respectively. A connection between cellulose polymorphs’ (cellulose I and cellulose II) contents (determined by XRD), fibers’ surface morphology (verified by FESEM), fabrics’ crimp, and capillarity of jute fabrics modified with 17.5 % NaOH was established. During the oxidative modifications, significant changes in jute fibers’ chemical composition and structure (i.e., lignin removal and more homogeneous middle lamellae) occurred which further resulted in enhanced jute fabrics’ water retention power and capillarity as well as fibers’ swelling up to 80 %, 75 %, and 54 %, compared to the raw jute, respectively. In order to move towards a circular economy and to ensure the recycling and re-use of recycled fabrics, the jute fabrics with enhanced sorption properties were evaluated as biosorbents for anthraquinone dye C. I. Acid Blue 111. The obtained results revealed that the jute fabrics’ maximum biosorption capacities for this dye ranged from 12.94 to 18.97 mg/g, while the equilibrium adsorption data were highly consistent with the Langmuir isotherm model. Moreover, based on the predicted dye pKa values, the fabric zeta potential, content of carboxyl and aldehyde groups as well as hydrogen bond intensity (determined by ATR-FTIR), a possible mechanism of the dye biosorption onto jute fabric waste was proposed.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle",
volume = "171",
doi = "10.1016/j.indcrop.2021.113913"
}

Ivanovska, A., Lađarević, J., Pavun, L., Antonijević, B., Cvijetić, I., Mijin, D.,& Kostić, M.. (2021). Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle. in Industrial Crops and Products
Elsevier B.V.., 171.
https://doi.org/10.1016/j.indcrop.2021.113913

Ivanovska A, Lađarević J, Pavun L, Antonijević B, Cvijetić I, Mijin D, Kostić M. Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle. in Industrial Crops and Products. 2021;171.
doi:10.1016/j.indcrop.2021.113913 .

Ivanovska, Aleksandra, Lađarević, Jelena, Pavun, Leposava, Antonijević, Biljana, Cvijetić, Ilija, Mijin, Dušan, Kostić, Mirjana, "Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle" in Industrial Crops and Products, 171 (2021),
https://doi.org/10.1016/j.indcrop.2021.113913 . .

TY  - CONF
AU  - Ivanovska, Aleksandra
AU  - Pavun, Leposava
AU  - Asanović, Koviljka
AU  - Kostić, Mirjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5215
AB  - Waste jute fabrics (raw and alkali modified), acquired as waste from different industries
were used as an efficient biosorbent for removal of Cu2+ ions from aqueous solution. All
woven jute fabrics (raw as well as alkali modified) were characterized by determination of
their chemical composition and amount of carboxyl groups. The effect of solution pH,
contact time and initial metal ion concentration on the biosorption were studied. The alkali
modifications lead to hemicelluloses removal and an increased amount of carboxyl groups.
The maximum biosorption capacity for Cu2+ was observed at pH 5.5. Concerning the contact
time, more than 80% of total Cu2+ uptake by the raw jute fabric was removed within 1 h. On
the other hand, in the case of alkali modified jute fabrics, between 70-75% of the total Cu2+
uptake was removed within 3 h. Increased initial ion concentration from 10 to 20 mg/l
caused an increase in the total uptake capacity of alkali modified jute fabrics for 42-55%. It
was found that the biosorption properties of waste jute fabrics are predominantly influenced
by the amount of carboxyl groups, while fabric chemical composition (i.e. hemicelluloses
content) has a secondary role in the biosorption of Cu2+ ions. The best biosorption
performance possesses alkali modified jute fabric with 58% higher amount of carboxyl
groups; its total uptake capacity towards Cu2+ ions (at 20 mg/l) is about 2 times higher
compared to the raw jute fabric.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Eighteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, December 4-6, 2019, Belgrade, Serbia
T1  - Biosorption of Cu2+ from aqueous solution by alkali modified waste jute woven fabrics
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5215
ER  - 

@conference{
author = "Ivanovska, Aleksandra and Pavun, Leposava and Asanović, Koviljka and Kostić, Mirjana",
year = "2019",
abstract = "Waste jute fabrics (raw and alkali modified), acquired as waste from different industries
were used as an efficient biosorbent for removal of Cu2+ ions from aqueous solution. All
woven jute fabrics (raw as well as alkali modified) were characterized by determination of
their chemical composition and amount of carboxyl groups. The effect of solution pH,
contact time and initial metal ion concentration on the biosorption were studied. The alkali
modifications lead to hemicelluloses removal and an increased amount of carboxyl groups.
The maximum biosorption capacity for Cu2+ was observed at pH 5.5. Concerning the contact
time, more than 80% of total Cu2+ uptake by the raw jute fabric was removed within 1 h. On
the other hand, in the case of alkali modified jute fabrics, between 70-75% of the total Cu2+
uptake was removed within 3 h. Increased initial ion concentration from 10 to 20 mg/l
caused an increase in the total uptake capacity of alkali modified jute fabrics for 42-55%. It
was found that the biosorption properties of waste jute fabrics are predominantly influenced
by the amount of carboxyl groups, while fabric chemical composition (i.e. hemicelluloses
content) has a secondary role in the biosorption of Cu2+ ions. The best biosorption
performance possesses alkali modified jute fabric with 58% higher amount of carboxyl
groups; its total uptake capacity towards Cu2+ ions (at 20 mg/l) is about 2 times higher
compared to the raw jute fabric.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Eighteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, December 4-6, 2019, Belgrade, Serbia",
title = "Biosorption of Cu2+ from aqueous solution by alkali modified waste jute woven fabrics",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5215"
}

Ivanovska, A., Pavun, L., Asanović, K.,& Kostić, M.. (2019). Biosorption of Cu2+ from aqueous solution by alkali modified waste jute woven fabrics. in Eighteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, December 4-6, 2019, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA..
https://hdl.handle.net/21.15107/rcub_farfar_5215

Ivanovska A, Pavun L, Asanović K, Kostić M. Biosorption of Cu2+ from aqueous solution by alkali modified waste jute woven fabrics. in Eighteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, December 4-6, 2019, Belgrade, Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_5215 .

Ivanovska, Aleksandra, Pavun, Leposava, Asanović, Koviljka, Kostić, Mirjana, "Biosorption of Cu2+ from aqueous solution by alkali modified waste jute woven fabrics" in Eighteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, December 4-6, 2019, Belgrade, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_farfar_5215 .

TY  - CONF
AU  - Spasojević-Dimitrijeva, Brankica
AU  - Kotur-Stevuljević, Jelena
AU  - Đukić, Milan
AU  - Mitrović, Jadranka
AU  - Cvetković, Mirjana
AU  - Miloševski-Lomić, Gordana
AU  - Paripović, Dušan
AU  - Gojković, Ivan
AU  - Kostić, Mirjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3079
PB  - Springer, New York
C3  - Pediatric Nephrology
T1  - Acute kidney injury after gadolinium and iodinated-based contrast media exposure in children with normal renal function
VL  - 33
IS  - 10
SP  - 1840
EP  - 1841
DO  - 10.1007/s00467-018-4028-x
ER  - 

@conference{
author = "Spasojević-Dimitrijeva, Brankica and Kotur-Stevuljević, Jelena and Đukić, Milan and Mitrović, Jadranka and Cvetković, Mirjana and Miloševski-Lomić, Gordana and Paripović, Dušan and Gojković, Ivan and Kostić, Mirjana",
year = "2018",
publisher = "Springer, New York",
journal = "Pediatric Nephrology",
title = "Acute kidney injury after gadolinium and iodinated-based contrast media exposure in children with normal renal function",
volume = "33",
number = "10",
pages = "1840-1841",
doi = "10.1007/s00467-018-4028-x"
}

Spasojević-Dimitrijeva, B., Kotur-Stevuljević, J., Đukić, M., Mitrović, J., Cvetković, M., Miloševski-Lomić, G., Paripović, D., Gojković, I.,& Kostić, M.. (2018). Acute kidney injury after gadolinium and iodinated-based contrast media exposure in children with normal renal function. in Pediatric Nephrology
Springer, New York., 33(10), 1840-1841.
https://doi.org/10.1007/s00467-018-4028-x

Spasojević-Dimitrijeva B, Kotur-Stevuljević J, Đukić M, Mitrović J, Cvetković M, Miloševski-Lomić G, Paripović D, Gojković I, Kostić M. Acute kidney injury after gadolinium and iodinated-based contrast media exposure in children with normal renal function. in Pediatric Nephrology. 2018;33(10):1840-1841.
doi:10.1007/s00467-018-4028-x .

Spasojević-Dimitrijeva, Brankica, Kotur-Stevuljević, Jelena, Đukić, Milan, Mitrović, Jadranka, Cvetković, Mirjana, Miloševski-Lomić, Gordana, Paripović, Dušan, Gojković, Ivan, Kostić, Mirjana, "Acute kidney injury after gadolinium and iodinated-based contrast media exposure in children with normal renal function" in Pediatric Nephrology, 33, no. 10 (2018):1840-1841,
https://doi.org/10.1007/s00467-018-4028-x . .

TY  - JOUR
AU  - Spasojević-Dimitrijeva, Brankica
AU  - Kotur-Stevuljević, Jelena
AU  - Dukić, Milan
AU  - Paripović, Dušan
AU  - Miloševski-Lomić, Gordana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Pavicević, Polina
AU  - Mitrović, Jadranka
AU  - Kostić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2793
AB  - Background: New renal biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) show promise in early diagnosis of contrast media induced acute kidney injury (CI-AKI). The purpose of our study was to compare the subclinical nephrotoxicity (a condition without changes in standard renal biomarkers) of gadolinium-based contrast media (Gd-DTPA, gadopentetate dimeglumine) and iodinated-based contrast media (iopromide) in pediatric patients with normal kidney function. Material/Methods: The first group (n=58) of patients included in the study were undergoing angiography with iopromide, and the second group (n=65) were undergoing magnetic resonance (MR) angiography/urography with Gd-DTPA administration. The concentrations of NGAL and KIM-1 were measured four times in the urine (pre-contrast, then at four hours, 24 hours, and 48 hours after contrast administration), and serum NGAL was measured at 0 (base-line), 24 hours, and 48 hours after contrast exposure. Results: After 24 hours, serum NGAL increase of >= 25% was noticed in 32.6% of the patients in the iopromide group and in 25.45% of the patients in the gadolinium group, with significantly higher average percent of this increase in first group (62.23% vs. 36.44%, p=0.002). In the Gd-DTPA group, we observed a statistically significant increase in urinary KIM-1 24 hours after the procedure. Normalized urinary KIM-1, 24 hours after contrast exposure, was a better predictive factor for CI-AKI than other biomarkers (AUC 0.757, cut off 214 pg/mg, sensitivity 83.3%, specificity 54.2%, p=0.035). Conclusions: In children with normal renal function, exposure to iodinated-based and gadolinium-based media might lead to subclinical nephrotoxicity, which could be detected using serum NGAL and urinary KIM-1.
PB  - Int Scientific Information, Inc, Melville
T2  - Medical Science Monitor
T1  - Serum Neutrophil Gelatinase-Associated Lipocalin and Urinary Kidney Injury Molecule-1 as Potential Biomarkers of Subclinical Nephrotoxicity After Gadolinium-Based and Iodinated-Based Contrast Media Exposure in Pediatric Patients with Normal Kidney Functio
VL  - 23
DO  - 10.12659/MSM.903255
ER  - 

@article{
author = "Spasojević-Dimitrijeva, Brankica and Kotur-Stevuljević, Jelena and Dukić, Milan and Paripović, Dušan and Miloševski-Lomić, Gordana and Spasojević-Kalimanovska, Vesna and Pavicević, Polina and Mitrović, Jadranka and Kostić, Mirjana",
year = "2017",
abstract = "Background: New renal biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) show promise in early diagnosis of contrast media induced acute kidney injury (CI-AKI). The purpose of our study was to compare the subclinical nephrotoxicity (a condition without changes in standard renal biomarkers) of gadolinium-based contrast media (Gd-DTPA, gadopentetate dimeglumine) and iodinated-based contrast media (iopromide) in pediatric patients with normal kidney function. Material/Methods: The first group (n=58) of patients included in the study were undergoing angiography with iopromide, and the second group (n=65) were undergoing magnetic resonance (MR) angiography/urography with Gd-DTPA administration. The concentrations of NGAL and KIM-1 were measured four times in the urine (pre-contrast, then at four hours, 24 hours, and 48 hours after contrast administration), and serum NGAL was measured at 0 (base-line), 24 hours, and 48 hours after contrast exposure. Results: After 24 hours, serum NGAL increase of >= 25% was noticed in 32.6% of the patients in the iopromide group and in 25.45% of the patients in the gadolinium group, with significantly higher average percent of this increase in first group (62.23% vs. 36.44%, p=0.002). In the Gd-DTPA group, we observed a statistically significant increase in urinary KIM-1 24 hours after the procedure. Normalized urinary KIM-1, 24 hours after contrast exposure, was a better predictive factor for CI-AKI than other biomarkers (AUC 0.757, cut off 214 pg/mg, sensitivity 83.3%, specificity 54.2%, p=0.035). Conclusions: In children with normal renal function, exposure to iodinated-based and gadolinium-based media might lead to subclinical nephrotoxicity, which could be detected using serum NGAL and urinary KIM-1.",
publisher = "Int Scientific Information, Inc, Melville",
journal = "Medical Science Monitor",
title = "Serum Neutrophil Gelatinase-Associated Lipocalin and Urinary Kidney Injury Molecule-1 as Potential Biomarkers of Subclinical Nephrotoxicity After Gadolinium-Based and Iodinated-Based Contrast Media Exposure in Pediatric Patients with Normal Kidney Functio",
volume = "23",
doi = "10.12659/MSM.903255"
}

Spasojević-Dimitrijeva, B., Kotur-Stevuljević, J., Dukić, M., Paripović, D., Miloševski-Lomić, G., Spasojević-Kalimanovska, V., Pavicević, P., Mitrović, J.,& Kostić, M.. (2017). Serum Neutrophil Gelatinase-Associated Lipocalin and Urinary Kidney Injury Molecule-1 as Potential Biomarkers of Subclinical Nephrotoxicity After Gadolinium-Based and Iodinated-Based Contrast Media Exposure in Pediatric Patients with Normal Kidney Functio. in Medical Science Monitor
Int Scientific Information, Inc, Melville., 23.
https://doi.org/10.12659/MSM.903255

Spasojević-Dimitrijeva B, Kotur-Stevuljević J, Dukić M, Paripović D, Miloševski-Lomić G, Spasojević-Kalimanovska V, Pavicević P, Mitrović J, Kostić M. Serum Neutrophil Gelatinase-Associated Lipocalin and Urinary Kidney Injury Molecule-1 as Potential Biomarkers of Subclinical Nephrotoxicity After Gadolinium-Based and Iodinated-Based Contrast Media Exposure in Pediatric Patients with Normal Kidney Functio. in Medical Science Monitor. 2017;23.
doi:10.12659/MSM.903255 .

Spasojević-Dimitrijeva, Brankica, Kotur-Stevuljević, Jelena, Dukić, Milan, Paripović, Dušan, Miloševski-Lomić, Gordana, Spasojević-Kalimanovska, Vesna, Pavicević, Polina, Mitrović, Jadranka, Kostić, Mirjana, "Serum Neutrophil Gelatinase-Associated Lipocalin and Urinary Kidney Injury Molecule-1 as Potential Biomarkers of Subclinical Nephrotoxicity After Gadolinium-Based and Iodinated-Based Contrast Media Exposure in Pediatric Patients with Normal Kidney Functio" in Medical Science Monitor, 23 (2017),
https://doi.org/10.12659/MSM.903255 . .

TY  - JOUR
AU  - Kotur-Stevuljević, Jelena
AU  - Peco-Antić, Amira
AU  - Spasić, Slavica
AU  - Stefanović, Aleksandra
AU  - Paripović, Dušan
AU  - Kostić, Mirjana
AU  - Vasić, Dragan
AU  - Vujović, Ana
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kornić-Ristovski, Danijela
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1943
AB  - The roles of dyslipidemia and oxidative stress in the early phases of atherosclerosis were tested in children with chronic kidney disease (CKD). Intima media thickness of common carotid arteries (cIMT) is used as a measure of early atherosclerosis. Fifty-two pediatric CKD patients were enrolled in the study (10 with chronic renal failure [CRF], 22 with a renal transplant [RT], 20 with chronic hemodialysis (cHD) patients, and 36 healthy children (control group, CG). Lipid status, oxidative stress, and paraoxonase 1 (PON1) status were assessed. cIMT was measured by ultrasound, adjusted for age and sex, and presented as standard deviation scores (SDS). Children with CKD had disturbed lipid content, which was most pronounced in cHD children, with higher free cholesterol and triglycerides compared with healthy children. Oxidative stress was markedly increased (malodialdehyde [MDA, mu mol/L]: CRF 1.50 +/- 0.26, RT 1.55 +/- 0.40, cHD 1.77 +/- 0.34, CG 0.97 +/- 0.33, p  lt  0.001) and antioxidative defense was compromised (superoxide dismutase [SOD, U/L]: CG 120 +/- 21, CRF 84 +/- 25, RT 93 +/- 12, cHD 119 +/- 37, p  lt  0.001). Multiple linear regression analysis showed that a model that included disease duration, blood pressure, urea, lipid, and oxidative status parameters accounted for more than 90% of the variability of cIMT-SDS. Early atherosclerosis in CKD children is caused, at least in part, by dyslipidemia and oxidative stress. Monitoring of vessel wall changes, along with assessment of oxidative stress status and high density lipoprotein (HDL) functionality is necessary to ensure better therapeutic strategies for delaying atherosclerotic changes in their asymptomatic phase.
PB  - Springer, New York
T2  - Pediatric Nephrology
T1  - Hyperlipidemia, oxidative stress, and intima media thickness in children with chronic kidney disease
VL  - 28
IS  - 2
SP  - 295
EP  - 303
DO  - 10.1007/s00467-012-2323-5
ER  - 

@article{
author = "Kotur-Stevuljević, Jelena and Peco-Antić, Amira and Spasić, Slavica and Stefanović, Aleksandra and Paripović, Dušan and Kostić, Mirjana and Vasić, Dragan and Vujović, Ana and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Kornić-Ristovski, Danijela",
year = "2013",
abstract = "The roles of dyslipidemia and oxidative stress in the early phases of atherosclerosis were tested in children with chronic kidney disease (CKD). Intima media thickness of common carotid arteries (cIMT) is used as a measure of early atherosclerosis. Fifty-two pediatric CKD patients were enrolled in the study (10 with chronic renal failure [CRF], 22 with a renal transplant [RT], 20 with chronic hemodialysis (cHD) patients, and 36 healthy children (control group, CG). Lipid status, oxidative stress, and paraoxonase 1 (PON1) status were assessed. cIMT was measured by ultrasound, adjusted for age and sex, and presented as standard deviation scores (SDS). Children with CKD had disturbed lipid content, which was most pronounced in cHD children, with higher free cholesterol and triglycerides compared with healthy children. Oxidative stress was markedly increased (malodialdehyde [MDA, mu mol/L]: CRF 1.50 +/- 0.26, RT 1.55 +/- 0.40, cHD 1.77 +/- 0.34, CG 0.97 +/- 0.33, p  lt  0.001) and antioxidative defense was compromised (superoxide dismutase [SOD, U/L]: CG 120 +/- 21, CRF 84 +/- 25, RT 93 +/- 12, cHD 119 +/- 37, p  lt  0.001). Multiple linear regression analysis showed that a model that included disease duration, blood pressure, urea, lipid, and oxidative status parameters accounted for more than 90% of the variability of cIMT-SDS. Early atherosclerosis in CKD children is caused, at least in part, by dyslipidemia and oxidative stress. Monitoring of vessel wall changes, along with assessment of oxidative stress status and high density lipoprotein (HDL) functionality is necessary to ensure better therapeutic strategies for delaying atherosclerotic changes in their asymptomatic phase.",
publisher = "Springer, New York",
journal = "Pediatric Nephrology",
title = "Hyperlipidemia, oxidative stress, and intima media thickness in children with chronic kidney disease",
volume = "28",
number = "2",
pages = "295-303",
doi = "10.1007/s00467-012-2323-5"
}

Kotur-Stevuljević, J., Peco-Antić, A., Spasić, S., Stefanović, A., Paripović, D., Kostić, M., Vasić, D., Vujović, A., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V.,& Kornić-Ristovski, D.. (2013). Hyperlipidemia, oxidative stress, and intima media thickness in children with chronic kidney disease. in Pediatric Nephrology
Springer, New York., 28(2), 295-303.
https://doi.org/10.1007/s00467-012-2323-5

Kotur-Stevuljević J, Peco-Antić A, Spasić S, Stefanović A, Paripović D, Kostić M, Vasić D, Vujović A, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Kornić-Ristovski D. Hyperlipidemia, oxidative stress, and intima media thickness in children with chronic kidney disease. in Pediatric Nephrology. 2013;28(2):295-303.
doi:10.1007/s00467-012-2323-5 .

Kotur-Stevuljević, Jelena, Peco-Antić, Amira, Spasić, Slavica, Stefanović, Aleksandra, Paripović, Dušan, Kostić, Mirjana, Vasić, Dragan, Vujović, Ana, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Kornić-Ristovski, Danijela, "Hyperlipidemia, oxidative stress, and intima media thickness in children with chronic kidney disease" in Pediatric Nephrology, 28, no. 2 (2013):295-303,
https://doi.org/10.1007/s00467-012-2323-5 . .

TY  - CONF
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Peco-Antić, Amira
AU  - Kostić, Mirjana
AU  - Vasić, Dragan
AU  - Spasić, Slavica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1460
PB  - Elsevier Science BV, Amsterdam
C3  - European Journal of Pharmaceutical Sciences
T1  - Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients
VL  - 44
IS  - Supplement 1
SP  - 191
EP  - 191
DO  - 10.1016/j.ejps.2011.08.002
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1460
ER  - 

@conference{
author = "Zeljković, Aleksandra and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Peco-Antić, Amira and Kostić, Mirjana and Vasić, Dragan and Spasić, Slavica",
year = "2011",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients",
volume = "44",
number = "Supplement 1",
pages = "191-191",
doi = "10.1016/j.ejps.2011.08.002",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1460"
}

Zeljković, A., Vekić, J., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Peco-Antić, A., Kostić, M., Vasić, D.,& Spasić, S.. (2011). Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 44(Supplement 1), 191-191.
https://doi.org/10.1016/j.ejps.2011.08.002
https://hdl.handle.net/21.15107/rcub_farfar_1460

Zeljković A, Vekić J, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Peco-Antić A, Kostić M, Vasić D, Spasić S. Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients. in European Journal of Pharmaceutical Sciences. 2011;44(Supplement 1):191-191.
doi:10.1016/j.ejps.2011.08.002
https://hdl.handle.net/21.15107/rcub_farfar_1460 .

Zeljković, Aleksandra, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Peco-Antić, Amira, Kostić, Mirjana, Vasić, Dragan, Spasić, Slavica, "Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients" in European Journal of Pharmaceutical Sciences, 44, no. Supplement 1 (2011):191-191,
https://doi.org/10.1016/j.ejps.2011.08.002 .,
https://hdl.handle.net/21.15107/rcub_farfar_1460 .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Peco-Antić, Amira
AU  - Kostić, Mirjana
AU  - Vasić, Dragan
AU  - Spasić, Slavica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1549
AB  - Renal transplant recipients often suffer from dyslipidemia which is one of the principal risk factors for cardiovascular disease. This study sought to determine characteristics of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particles and their associations with carotid intima-media thickness (cIMT) in a group of pediatric renal transplant recipients. We also examined the influence of immunosuppressive therapy on measured LDL and HDL particle characteristics. HDL size and subclass distribution were determined using gradient gel electrophoresis, while concentrations of small, dense LDL (sdLDL)-cholesterol (sdLDL-C) and sdLDL-apolipoprotein B (sdLDL-apoB) using heparin-magnesium precipitation method in 21 renal transplant recipients and 32 controls. Renal transplant recipients had less HDL 2b (P  lt  0.001), but more HDL 3a (P  lt  0.01) and 3b (P  lt  0.001) subclasses. They also had increased sdLDL-C (P  lt  0.01) and sdLDL-apoB (P  lt  0.05) levels. The proportion of the HDL 3b subclasses was a significant predictor of increased cIMT (P  lt  0.05). Patients treated with cyclosporine had significantly higher sdLDL-C and sdLDL-apoB concentrations (P  lt  0.05) when compared with those on tacrolimus therapy. Pediatric renal transplant recipients have impaired distribution of HDL and LDL particles. Changes in the proportion of small-sized HDL particles are significantly associated with cIMT. Advanced lipid testing might be useful in evaluating the effects of immunosuppressive therapy.
PB  - Wiley-Blackwell, Malden
T2  - Transplantation
T1  - Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients
VL  - 24
IS  - 11
SP  - 1094
EP  - 1102
DO  - 10.1111/j.1432-2277.2011.01313.x
ER  - 

@article{
author = "Zeljković, Aleksandra and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Peco-Antić, Amira and Kostić, Mirjana and Vasić, Dragan and Spasić, Slavica",
year = "2011",
abstract = "Renal transplant recipients often suffer from dyslipidemia which is one of the principal risk factors for cardiovascular disease. This study sought to determine characteristics of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particles and their associations with carotid intima-media thickness (cIMT) in a group of pediatric renal transplant recipients. We also examined the influence of immunosuppressive therapy on measured LDL and HDL particle characteristics. HDL size and subclass distribution were determined using gradient gel electrophoresis, while concentrations of small, dense LDL (sdLDL)-cholesterol (sdLDL-C) and sdLDL-apolipoprotein B (sdLDL-apoB) using heparin-magnesium precipitation method in 21 renal transplant recipients and 32 controls. Renal transplant recipients had less HDL 2b (P  lt  0.001), but more HDL 3a (P  lt  0.01) and 3b (P  lt  0.001) subclasses. They also had increased sdLDL-C (P  lt  0.01) and sdLDL-apoB (P  lt  0.05) levels. The proportion of the HDL 3b subclasses was a significant predictor of increased cIMT (P  lt  0.05). Patients treated with cyclosporine had significantly higher sdLDL-C and sdLDL-apoB concentrations (P  lt  0.05) when compared with those on tacrolimus therapy. Pediatric renal transplant recipients have impaired distribution of HDL and LDL particles. Changes in the proportion of small-sized HDL particles are significantly associated with cIMT. Advanced lipid testing might be useful in evaluating the effects of immunosuppressive therapy.",
publisher = "Wiley-Blackwell, Malden",
journal = "Transplantation",
title = "Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients",
volume = "24",
number = "11",
pages = "1094-1102",
doi = "10.1111/j.1432-2277.2011.01313.x"
}

Zeljković, A., Vekić, J., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Peco-Antić, A., Kostić, M., Vasić, D.,& Spasić, S.. (2011). Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients. in Transplantation
Wiley-Blackwell, Malden., 24(11), 1094-1102.
https://doi.org/10.1111/j.1432-2277.2011.01313.x

Zeljković A, Vekić J, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Peco-Antić A, Kostić M, Vasić D, Spasić S. Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients. in Transplantation. 2011;24(11):1094-1102.
doi:10.1111/j.1432-2277.2011.01313.x .

Zeljković, Aleksandra, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Peco-Antić, Amira, Kostić, Mirjana, Vasić, Dragan, Spasić, Slavica, "Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients" in Transplantation, 24, no. 11 (2011):1094-1102,
https://doi.org/10.1111/j.1432-2277.2011.01313.x . .

TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kostić, Mirjana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1255
AB  - Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of mycophenolic acid (MPA) and mycophenolic acid glucuronide (MPAG) in human urine and plasma. In the first stage of method development, pH value of the water phase. percentage of acetonitrile, temperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. Afterwards, the optimal conditions were found employing central composite design and Derringer's desirability function. Two goals were considered, the retention factor of the MPAG to be in the range, between 0.8 and 1.118 which allowed well separation of MPAG from the urine and plasma peaks, and the shortest possible total analysis run time. Then, the obtained sigmoid functions were used to transform the optimization criteria into the desirability values. The satisfactory chromatographic conditions were obtained with mobile phase consisted of acetonitrile-phosphate buffer (pH 2.4; 0.04 M KH2PO4) (28:72, v/v). The separation was performed on C-18 Chromolith column (100 mm x 4.6 mm) with flow rate of 5 mL/min, the temperature of the column was 25 degrees C and the chosen wavelength for simultaneous determination of MPA and MPAG was 215 nm. The MPAG eluted at 0.552 min and the duration of run was 3.092 min. Afterwards, the method was subjected to validation. Linearity was observed over the concentration range of 1-50 mu g/mL for MPA and 1-500 mu g/mL for MPAG in urine and 1-60 mu g/mL for MPA and 1-70 mu g/mL for MPAG in plasma matrix. The method showed intra-day and inter-day precision with relative standard deviation lower then 5% and accuracy as recovery (%) between 100 +/- 5%.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma
VL  - 50
IS  - 4
SP  - 640
EP  - 648
DO  - 10.1016/j.jpba.2008.09.052
ER  - 

@article{
author = "Živanović, Ljiljana and Protić, Ana and Zečević, Mira and Jocić, Biljana and Kostić, Mirjana",
year = "2009",
abstract = "Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of mycophenolic acid (MPA) and mycophenolic acid glucuronide (MPAG) in human urine and plasma. In the first stage of method development, pH value of the water phase. percentage of acetonitrile, temperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. Afterwards, the optimal conditions were found employing central composite design and Derringer's desirability function. Two goals were considered, the retention factor of the MPAG to be in the range, between 0.8 and 1.118 which allowed well separation of MPAG from the urine and plasma peaks, and the shortest possible total analysis run time. Then, the obtained sigmoid functions were used to transform the optimization criteria into the desirability values. The satisfactory chromatographic conditions were obtained with mobile phase consisted of acetonitrile-phosphate buffer (pH 2.4; 0.04 M KH2PO4) (28:72, v/v). The separation was performed on C-18 Chromolith column (100 mm x 4.6 mm) with flow rate of 5 mL/min, the temperature of the column was 25 degrees C and the chosen wavelength for simultaneous determination of MPA and MPAG was 215 nm. The MPAG eluted at 0.552 min and the duration of run was 3.092 min. Afterwards, the method was subjected to validation. Linearity was observed over the concentration range of 1-50 mu g/mL for MPA and 1-500 mu g/mL for MPAG in urine and 1-60 mu g/mL for MPA and 1-70 mu g/mL for MPAG in plasma matrix. The method showed intra-day and inter-day precision with relative standard deviation lower then 5% and accuracy as recovery (%) between 100 +/- 5%.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma",
volume = "50",
number = "4",
pages = "640-648",
doi = "10.1016/j.jpba.2008.09.052"
}

Živanović, L., Protić, A., Zečević, M., Jocić, B.,& Kostić, M.. (2009). Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 50(4), 640-648.
https://doi.org/10.1016/j.jpba.2008.09.052

Živanović L, Protić A, Zečević M, Jocić B, Kostić M. Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma. in Journal of Pharmaceutical and Biomedical Analysis. 2009;50(4):640-648.
doi:10.1016/j.jpba.2008.09.052 .

Živanović, Ljiljana, Protić, Ana, Zečević, Mira, Jocić, Biljana, Kostić, Mirjana, "Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma" in Journal of Pharmaceutical and Biomedical Analysis, 50, no. 4 (2009):640-648,
https://doi.org/10.1016/j.jpba.2008.09.052 . .

TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Licanski, A.
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kostić, Mirjana
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1089
AB  - The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation
VL  - 47
IS  - 3
SP  - 575
EP  - 585
DO  - 10.1016/j.jpba.2008.01.046
ER  - 

@article{
author = "Živanović, Ljiljana and Licanski, A. and Zečević, Mira and Jocić, Biljana and Kostić, Mirjana",
year = "2008",
abstract = "The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation",
volume = "47",
number = "3",
pages = "575-585",
doi = "10.1016/j.jpba.2008.01.046"
}

Živanović, L., Licanski, A., Zečević, M., Jocić, B.,& Kostić, M.. (2008). Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 47(3), 575-585.
https://doi.org/10.1016/j.jpba.2008.01.046

Živanović L, Licanski A, Zečević M, Jocić B, Kostić M. Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis. 2008;47(3):575-585.
doi:10.1016/j.jpba.2008.01.046 .

Živanović, Ljiljana, Licanski, A., Zečević, Mira, Jocić, Biljana, Kostić, Mirjana, "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation" in Journal of Pharmaceutical and Biomedical Analysis, 47, no. 3 (2008):575-585,
https://doi.org/10.1016/j.jpba.2008.01.046 . .