@article{
author = "Žilić, Slađana and Milivojević, Marija and Šobajić, Slađana and Maksimović, Milan",
year = "2006",
abstract = "The purpose of this study was to determine changes of lipoxygenase activities, contents of antioxidants (tocopherol α, β-carotene and chlorophyll) fatty acids and soluble proteins, as well as, vigor in accelerated aged soybean seeds. The following soybean cultivars were used in the study: Williams 82, Goyou Kurakake and L93-7290. Subsequent to the accelerated ageing test (AAT), 23% of seeds of the cultivar Goyou Kurakake retained normal germination, while grain of the two remaining cultivars L93-7290 and Williams 82, completely lost germination ability. According to our results, the seeds of the cultivar Goyou Kurakake (the absence of lipoxygenases 2) is characterized by a significantly higher content of all observed antioxidants (tocopherol α 2.7 mg 100g-1, β-carotene 6.1 µg g-1 chlorophyll 4.9 µg g-1). The contents of (β-carotene and chlorophyll decreased after AAT in the seed of the cultivar Goyou Kurakake by 42.8% and 60.5%, respectively, while the content of tocopherol a remained the same. Furthermore, the tocopherol a content was not changed after AAT neither in the cultivar L93-7290 nor in the cultivar Williams 82. The content of β-carotene after AAT remained the same in seeds of the cultivar Williams 82 while it decreased by 7.7% in the cultivar L93-7290. Since changes of the fatty acids content were not pronounced in the cultivar Goyou Kurakake after AAT it can be concluded that the antioxidative system had a crucial role in seeds protection against lipid peroxidation., Svrha ovih istraživanja bila je da se odrede promene aktivnosti izoenzima lipoksigenaze, sadržaja antioksidanasa (tokoferola α, ß-karotina i hlorofila), masnih kiselina i rastvorljivih proteina, kao i životne sposobnosti semena soje nakon ubrzanog starenja. Za istraživanja su korišćene sledeće sorte soje: Williams 82, Goyou Kurakake and L93-7290. Nakon testa ubrzanog starenja 23% semena sorte Goyou Kurakake zadržalo je normalnu klijavost, dok je seme sorti Williams 82 i L93-7290 potpuno izgubilo klijavost. Na osnovu naših rezultata sorta Goyou Kurakake, koja se odlikuje odsustvom lipoksigenaze 2, imala je značajno viši sadržaj ispitivanih antioksidanasa (tokofherol α 2,7 mg 100g-1, ß-karotina 6,1 µg g-1, hlorofila 4,9 µg g-1). Sadržaj ß-karotina i hlorofila, nakon testa ubrzanog starenja smanjio se za 42,8% odnosno 60,5%, dok je sadržaj tokoferol α ostao nepromenjen. Sadržaj tokoferol α ostao je nepromenjen i u semenu sorti Williams 82 i L93-7290 nakon ubrzanog starenja. Nakon testa ubrzanog starenja u semenu sorte Williams 82 sadržaj ß-karotina je ostao isti, dok je u semenu sorte L93-7290 smanjen za 7,7%. S obzirom da nakon testa ubrzanog starenja nije došlo do promene sadržaja masnih kiselina kod sorte Goyou Kurakake, može se zaključiti da antioksidacioni sistem ima značajnu ulogu u zaštiti od lipidne peroksidacije.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test, Uticaj multiplih alela na oksidacionu stabilnost i klijavost semena soje nakon ubrzanog starenja",
volume = "38",
number = "1",
pages = "37-48",
doi = "10.2298/GENSR0601037Z"
}
