TY  - JOUR
AU  - Tosić, Svetlana
AU  - Stojicić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3363
AB  - Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer, New York
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
VL  - 249
IS  - 5
SP  - 1365
EP  - 1377
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tosić, Svetlana and Stojicić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer, New York",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
volume = "249",
number = "5",
pages = "1365-1377",
doi = "10.1007/s00425-018-03071-5"
}

Tosić, S., Stojicić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta
Springer, New York., 249(5), 1365-1377.
https://doi.org/10.1007/s00425-018-03071-5

Tosić S, Stojicić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta. 2019;249(5):1365-1377.
doi:10.1007/s00425-018-03071-5 .

Tosić, Svetlana, Stojicić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Planta, 249, no. 5 (2019):1365-1377,
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Veljković, Milica
AU  - Stojanović, Nikola M.
AU  - Gocmanac-Ignjatović, Marija
AU  - Mihailov-Krstev, Tatjana
AU  - Branković, Suzana
AU  - Sokolović, Dušan
AU  - Marčetić, Mirjana
AU  - Radulović, Niko
AU  - Radenković, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2802
AB  - ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.
PB  - Wiley, Hoboken
T2  - Journal of Pharmacy and Pharmacology
T1  - Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties
VL  - 69
IS  - 9
SP  - 1208
EP  - 1218
DO  - 10.1111/jphp.12746
ER  - 

@article{
author = "Pavlović, Dragana R. and Veljković, Milica and Stojanović, Nikola M. and Gocmanac-Ignjatović, Marija and Mihailov-Krstev, Tatjana and Branković, Suzana and Sokolović, Dušan and Marčetić, Mirjana and Radulović, Niko and Radenković, Mirjana",
year = "2017",
abstract = "ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.",
publisher = "Wiley, Hoboken",
journal = "Journal of Pharmacy and Pharmacology",
title = "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties",
volume = "69",
number = "9",
pages = "1208-1218",
doi = "10.1111/jphp.12746"
}

Pavlović, D. R., Veljković, M., Stojanović, N. M., Gocmanac-Ignjatović, M., Mihailov-Krstev, T., Branković, S., Sokolović, D., Marčetić, M., Radulović, N.,& Radenković, M.. (2017). Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology
Wiley, Hoboken., 69(9), 1208-1218.
https://doi.org/10.1111/jphp.12746

Pavlović DR, Veljković M, Stojanović NM, Gocmanac-Ignjatović M, Mihailov-Krstev T, Branković S, Sokolović D, Marčetić M, Radulović N, Radenković M. Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology. 2017;69(9):1208-1218.
doi:10.1111/jphp.12746 .

Pavlović, Dragana R., Veljković, Milica, Stojanović, Nikola M., Gocmanac-Ignjatović, Marija, Mihailov-Krstev, Tatjana, Branković, Suzana, Sokolović, Dušan, Marčetić, Mirjana, Radulović, Niko, Radenković, Mirjana, "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties" in Journal of Pharmacy and Pharmacology, 69, no. 9 (2017):1208-1218,
https://doi.org/10.1111/jphp.12746 . .