TY  - JOUR
AU  - Maksić, Jelena
AU  - Stajić, Ana
AU  - Knežević, Miroslav
AU  - Dacić-Krnjaja, Bojana
AU  - Jančić-Stojanović, Biljana
AU  - Medenica, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2860
AB  - Aim: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. Materials & methods: Separation was performed on Acquity BEH amide column (2.1 x 100 mm, 1.7 mu m). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized. Results: The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated. Conclusion: The proposed method was utilized for monitoring of olopatadine in human tear.
PB  - Future Sci Ltd, London
T2  - Bioanalysis
T1  - Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method
VL  - 9
IS  - 24
SP  - 1943
EP  - 1954
DO  - 10.4155/bio-2017-0172
ER  - 

@article{
author = "Maksić, Jelena and Stajić, Ana and Knežević, Miroslav and Dacić-Krnjaja, Bojana and Jančić-Stojanović, Biljana and Medenica, Mirjana",
year = "2017",
abstract = "Aim: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. Materials & methods: Separation was performed on Acquity BEH amide column (2.1 x 100 mm, 1.7 mu m). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized. Results: The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated. Conclusion: The proposed method was utilized for monitoring of olopatadine in human tear.",
publisher = "Future Sci Ltd, London",
journal = "Bioanalysis",
title = "Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method",
volume = "9",
number = "24",
pages = "1943-1954",
doi = "10.4155/bio-2017-0172"
}

Maksić, J., Stajić, A., Knežević, M., Dacić-Krnjaja, B., Jančić-Stojanović, B.,& Medenica, M.. (2017). Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method. in Bioanalysis
Future Sci Ltd, London., 9(24), 1943-1954.
https://doi.org/10.4155/bio-2017-0172

Maksić J, Stajić A, Knežević M, Dacić-Krnjaja B, Jančić-Stojanović B, Medenica M. Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method. in Bioanalysis. 2017;9(24):1943-1954.
doi:10.4155/bio-2017-0172 .

Maksić, Jelena, Stajić, Ana, Knežević, Miroslav, Dacić-Krnjaja, Bojana, Jančić-Stojanović, Biljana, Medenica, Mirjana, "Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method" in Bioanalysis, 9, no. 24 (2017):1943-1954,
https://doi.org/10.4155/bio-2017-0172 . .

TY  - JOUR
AU  - Foivas, Anargyros
AU  - Malenović, Anđelija
AU  - Kostić, Nada
AU  - Božić, Marija
AU  - Knežević, Miroslav
AU  - Loukas, Yannis L.
AU  - Dotsikas, Yannis
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2566
AB  - In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method
VL  - 119
SP  - 84
EP  - 90
DO  - 10.1016/j.jpba.2015.11.043
ER  - 

@article{
author = "Foivas, Anargyros and Malenović, Anđelija and Kostić, Nada and Božić, Marija and Knežević, Miroslav and Loukas, Yannis L. and Dotsikas, Yannis",
year = "2016",
abstract = "In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method",
volume = "119",
pages = "84-90",
doi = "10.1016/j.jpba.2015.11.043"
}

Foivas, A., Malenović, A., Kostić, N., Božić, M., Knežević, M., Loukas, Y. L.,& Dotsikas, Y.. (2016). Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 119, 84-90.
https://doi.org/10.1016/j.jpba.2015.11.043

Foivas A, Malenović A, Kostić N, Božić M, Knežević M, Loukas YL, Dotsikas Y. Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis. 2016;119:84-90.
doi:10.1016/j.jpba.2015.11.043 .

Foivas, Anargyros, Malenović, Anđelija, Kostić, Nada, Božić, Marija, Knežević, Miroslav, Loukas, Yannis L., Dotsikas, Yannis, "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method" in Journal of Pharmaceutical and Biomedical Analysis, 119 (2016):84-90,
https://doi.org/10.1016/j.jpba.2015.11.043 . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Knežević, Miroslav
AU  - Božić, Dragana
AU  - Rasić, Dejan
AU  - Larsen, Anders Rhod
AU  - Dukić, Slobodanka
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2414
AB  - The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.
PB  - Springer, New York
T2  - Graefes Archive for Clinical and Experimental Ophthalmology
T1  - Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage
VL  - 253
IS  - 1
SP  - 77
EP  - 82
DO  - 10.1007/s00417-014-2786-0
ER  - 

@article{
author = "Cirković, Ivana and Knežević, Miroslav and Božić, Dragana and Rasić, Dejan and Larsen, Anders Rhod and Dukić, Slobodanka",
year = "2015",
abstract = "The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.",
publisher = "Springer, New York",
journal = "Graefes Archive for Clinical and Experimental Ophthalmology",
title = "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage",
volume = "253",
number = "1",
pages = "77-82",
doi = "10.1007/s00417-014-2786-0"
}

Cirković, I., Knežević, M., Božić, D., Rasić, D., Larsen, A. R.,& Dukić, S.. (2015). Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology
Springer, New York., 253(1), 77-82.
https://doi.org/10.1007/s00417-014-2786-0

Cirković I, Knežević M, Božić D, Rasić D, Larsen AR, Dukić S. Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology. 2015;253(1):77-82.
doi:10.1007/s00417-014-2786-0 .

Cirković, Ivana, Knežević, Miroslav, Božić, Dragana, Rasić, Dejan, Larsen, Anders Rhod, Dukić, Slobodanka, "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage" in Graefes Archive for Clinical and Experimental Ophthalmology, 253, no. 1 (2015):77-82,
https://doi.org/10.1007/s00417-014-2786-0 . .