TY  - JOUR
AU  - Bošnjak-Neumueller, Jasna
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Vuković-Gacić, Branka
AU  - Dajić-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knezević-Vukcević, Jelena
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2994
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).
PB  - Univ Karachi, Karachi
T2  - Pakistan journal of pharmaceutical sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
VL  - 30
IS  - 2
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumueller, Jasna and Radaković, Milena and Đelić, Ninoslav and Vuković-Gacić, Branka and Dajić-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knezević-Vukcević, Jelena and Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).",
publisher = "Univ Karachi, Karachi",
journal = "Pakistan journal of pharmaceutical sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
volume = "30",
number = "2",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumueller, J., Radaković, M., Đelić, N., Vuković-Gacić, B., Dajić-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knezević-Vukcević, J., Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences
Univ Karachi, Karachi., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumueller J, Radaković M, Đelić N, Vuković-Gacić B, Dajić-Stevanović Z, Kolarević S, Misić D, Stanković M, Knezević-Vukcević J, Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumueller, Jasna, Radaković, Milena, Đelić, Ninoslav, Vuković-Gacić, Branka, Dajić-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knezević-Vukcević, Jelena, Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan journal of pharmaceutical sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Šoštarić, Ivan
AU  - Arsenijević, Jelena
AU  - Acić, Svetlana
AU  - Dajić-Stevanović, Zora
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1669
AB  - Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia
VL  - 15
IS  - 2
SP  - 237
EP  - 243
DO  - 10.1080/0972060X.2012.10644041
ER  - 

@article{
author = "Šoštarić, Ivan and Arsenijević, Jelena and Acić, Svetlana and Dajić-Stevanović, Zora",
year = "2012",
abstract = "Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia",
volume = "15",
number = "2",
pages = "237-243",
doi = "10.1080/0972060X.2012.10644041"
}

Šoštarić, I., Arsenijević, J., Acić, S.,& Dajić-Stevanović, Z.. (2012). Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 15(2), 237-243.
https://doi.org/10.1080/0972060X.2012.10644041

Šoštarić I, Arsenijević J, Acić S, Dajić-Stevanović Z. Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants. 2012;15(2):237-243.
doi:10.1080/0972060X.2012.10644041 .

Šoštarić, Ivan, Arsenijević, Jelena, Acić, Svetlana, Dajić-Stevanović, Zora, "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia" in Journal of Essential Oil Bearing Plants, 15, no. 2 (2012):237-243,
https://doi.org/10.1080/0972060X.2012.10644041 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Šoštarić, Ivan
AU  - Marin, Petar
AU  - Stojanović, Danilo
AU  - Ristić, Mihailo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1143
AB  - In five indigenous populations of Thymus glabrescens Willd. collected in the region of Banat (Serbia), the variability on leaf morphological traits, leaf and stem anatomy, and composition of the essential oil was studied. The major component in the studied populations was either thymol or γ-terpinene. Distinct differentiation of populations with respect to chemical composition of essential oils might be related to spatial distribution of the studied populations. No correlations between morphology, anatomy, and essential oil yield and composition were determined. Both capitate and peltate glandular trichomes were found on calyces, whereas the latter were noticed on the abaxial and adaxial leaf surface.
AB  - U pet autohtonih populacija Thymus glabrescens Willd. sakupljenih u regionu Banata, Srbija, ispitivana je varijabilnost morfoloških karakteristika lista, anatomske građe lista i stabla i sastava etarskih ulja. Glavne komponente etarskih ulja proučavanih populacija bile su timol, γ-terpinen i p-cimen. Uočena je prostorna diferencijacija populacija u odnosu na sastav etarskih ulja. Nije ustanovljena korelacija između morfoloških i anatomskih karakteristika populacija i prinosa i sastava etarskih ulja. Prisustvo i izgled žlezdanih trihoma su utvrđeni posmatranjem nadzemnih delova na svetlosnom i skening elektronskom mikroskopu. Peltatne trihome su uočene na čašici, licu i naličju lista, dok su kapitatne nađene samo na čašicama.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition
T1  - Populaciona varijabilnost unutar vrste Thymus glabrescens Willd. iz Srbije - morfologija, anatomija i sastav etarskih ulja
VL  - 60
IS  - 3
SP  - 475
EP  - 483
DO  - 10.2298/ABS0803475D
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Šoštarić, Ivan and Marin, Petar and Stojanović, Danilo and Ristić, Mihailo",
year = "2008",
abstract = "In five indigenous populations of Thymus glabrescens Willd. collected in the region of Banat (Serbia), the variability on leaf morphological traits, leaf and stem anatomy, and composition of the essential oil was studied. The major component in the studied populations was either thymol or γ-terpinene. Distinct differentiation of populations with respect to chemical composition of essential oils might be related to spatial distribution of the studied populations. No correlations between morphology, anatomy, and essential oil yield and composition were determined. Both capitate and peltate glandular trichomes were found on calyces, whereas the latter were noticed on the abaxial and adaxial leaf surface., U pet autohtonih populacija Thymus glabrescens Willd. sakupljenih u regionu Banata, Srbija, ispitivana je varijabilnost morfoloških karakteristika lista, anatomske građe lista i stabla i sastava etarskih ulja. Glavne komponente etarskih ulja proučavanih populacija bile su timol, γ-terpinen i p-cimen. Uočena je prostorna diferencijacija populacija u odnosu na sastav etarskih ulja. Nije ustanovljena korelacija između morfoloških i anatomskih karakteristika populacija i prinosa i sastava etarskih ulja. Prisustvo i izgled žlezdanih trihoma su utvrđeni posmatranjem nadzemnih delova na svetlosnom i skening elektronskom mikroskopu. Peltatne trihome su uočene na čašici, licu i naličju lista, dok su kapitatne nađene samo na čašicama.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition, Populaciona varijabilnost unutar vrste Thymus glabrescens Willd. iz Srbije - morfologija, anatomija i sastav etarskih ulja",
volume = "60",
number = "3",
pages = "475-483",
doi = "10.2298/ABS0803475D"
}

Dajić-Stevanović, Z., Šoštarić, I., Marin, P., Stojanović, D.,& Ristić, M.. (2008). Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 475-483.
https://doi.org/10.2298/ABS0803475D

Dajić-Stevanović Z, Šoštarić I, Marin P, Stojanović D, Ristić M. Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition. in Archives of Biological Sciences. 2008;60(3):475-483.
doi:10.2298/ABS0803475D .

Dajić-Stevanović, Zora, Šoštarić, Ivan, Marin, Petar, Stojanović, Danilo, Ristić, Mihailo, "Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition" in Archives of Biological Sciences, 60, no. 3 (2008):475-483,
https://doi.org/10.2298/ABS0803475D . .

TY  - JOUR
AU  - Maksimović, Zoran
AU  - Stojanović, Danilo
AU  - Šoštarić, Ivan
AU  - Dajić, Zora
AU  - Ristić, Mihailo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1117
AB  - BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil
VL  - 88
IS  - 11
SP  - 2036
EP  - 2041
DO  - 10.1002/jsfa.3311
ER  - 

@article{
author = "Maksimović, Zoran and Stojanović, Danilo and Šoštarić, Ivan and Dajić, Zora and Ristić, Mihailo",
year = "2008",
abstract = "BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil",
volume = "88",
number = "11",
pages = "2036-2041",
doi = "10.1002/jsfa.3311"
}

Maksimović, Z., Stojanović, D., Šoštarić, I., Dajić, Z.,& Ristić, M.. (2008). Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 88(11), 2036-2041.
https://doi.org/10.1002/jsfa.3311

Maksimović Z, Stojanović D, Šoštarić I, Dajić Z, Ristić M. Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture. 2008;88(11):2036-2041.
doi:10.1002/jsfa.3311 .

Maksimović, Zoran, Stojanović, Danilo, Šoštarić, Ivan, Dajić, Zora, Ristić, Mihailo, "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil" in Journal of the Science of Food and Agriculture, 88, no. 11 (2008):2036-2041,
https://doi.org/10.1002/jsfa.3311 . .