@conference{
author = "Milutinović, Violeta and Petrović, Silvana and Niketić, Marjan and Krunić, Aleksej and Nikolić, Dejan",
year = "2018",
abstract = "The genus Hieracium L. is autochthonous in Eurasia, North Africa and North America and the
vast majority of the taxa are distributed in Europe. Hieracium naegelianum Pančić, belonging
to monotypic subendemic section H. sect. Naegeliana Zahn ex Szeląg, is a small, caespitose
plant, with long rhizome. It inhabits subalpine and alpine orosubmediterranean screes and
mainly is endemic for the mountains in W Balkan, with a single population in S Apennines.
Hieracium scheppigianum Freyn is a hybridogenous apomictic species probably originated
from H. gymnocephalum Griseb. ex Pant. (H. sect. Pannosa /Zahn (Zahn) and H. bupleuroides
C.C. Gmelin, s. l. (H. sect. Drepanoidea Monnier). The species can be found only on screes,
rocky places or rocky pastures in subalpine and alpine zone in Dinarides. The underground
parts of H. naegelianum and H. scheppigianum were collected on Mt Durmitor, Republic of
Montenegro. Dried and powered plant material was macerated with dichloromethane and
methanol, successively. Both solvents were evaporated under reduced pressure and dried
methanol extracts were used for further investigation. Using LC-MS method, sesquiterpene
lactone glycoside of guaianolide type, crepiside E, and six phenolic acids, i.e. chlorogenic acid
and five dicaffeoylquinic acids (cynarin, 3,5-, 1,5-, 3,4- and 4,5-dicaffeoylquinic acids) were
identified and quantified in both dried methanol extracts. Crepiside E was the most abundant
compound (116.58 and 126.88 mg/g), while among phenolic acids, 3,5-dicaffeoylquinic
acid (72.86 and 62.01 mg/g) was dominant in both investigated extracts. Radical scavenging
activity was estimated using colorimetric DPPH and OH radical assays, and both extracts
showed prominent and concentration dependent activity with SC50DPPH of 25.25 and
29.38 μg/mL and SC50OH of 16.09 and 17.50 μg/mL, respectively. This is the first report on
secondary metabolites and antiradical potential of underground parts of H. naegelianum and
H. scheppigianum",
publisher = "University of Zagreb, Faculty of Agriculture, Zagreb, Croatia, Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Zagreb, Croatia",
journal = "10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts",
title = "Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5260"
}