TY  - JOUR
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Nikolić, Dejan
AU  - Krunić, Aleksej
AU  - Zidorn, Christian
AU  - Petrović, Silvana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3127
AB  - IntroductionHieracium s. str. represents one of the largest and most complex genera of flowering plants. As molecular genetics seems unlikely to disentangle intricate relationships within this reticulate species complex, analysis of flavonoids and phenolic acids, known as good chemosystematic markers, promise to be more reliable. Data about pharmacological activity of Hieracium species are scarce. ObjectiveEvaluation of the chemosystematic significance of flavonoids and phenolic acids of methanol extracts of aerial flowering parts of 28 Hieracium species from the Balkans. Additionally, investigation of antioxidant potentials of the extracts. MethodsComparative qualitative and quantitative analysis of flavonoids and phenolic acids was performed by LC-MS. Multivariate statistical data analysis included non-metric multidimensional scaling (nMDS), unweighted pair-group arithmetic averages (UPGMA) and principal component analysis (PCA). Antioxidant activity was evaluated using three colorimetric tests. ResultsDominant phenolics in almost all species were luteolin type flavonoids, followed by phenolic acids. Although the investigated Hieracium species share many compounds, the current classification of the genus was supported by nMDS and UPGMA analyses with a good resolution to the group level. Hieracium naegelianum was clearly separated from the other investigated species. Spatial and ecological distances of the samples were likely to influence unexpected differentiation of some groups within H. sect. Pannosa. The vast majority of dominant compounds significantly contributed to differences between taxa. The antioxidant potential of the extracts was satisfactory and in accordance with their phenolics composition. ConclusionsComparative LC-MS analysis demonstrated that flavonoids and phenolic acids are good indicators of chemosystematic relationships within Hieracium, particularly between non-hybrid species and groups from the same location. Copyright
PB  - Wiley, Hoboken
T2  - Phytochemical Analysis
T1  - Methanol Extracts of 28 Hieracium Species from the Balkan Peninsula - Comparative LC-MS Analysis, Chemosystematic Evaluation of their Flavonoid and Phenolic Acid Profiles and Antioxidant Potentials
VL  - 29
IS  - 1
SP  - 30
EP  - 47
DO  - 10.1002/pca.2712
ER  - 

@article{
author = "Milutinović, Violeta and Niketić, Marjan and Ušjak, Ljuboš and Nikolić, Dejan and Krunić, Aleksej and Zidorn, Christian and Petrović, Silvana",
year = "2018",
abstract = "IntroductionHieracium s. str. represents one of the largest and most complex genera of flowering plants. As molecular genetics seems unlikely to disentangle intricate relationships within this reticulate species complex, analysis of flavonoids and phenolic acids, known as good chemosystematic markers, promise to be more reliable. Data about pharmacological activity of Hieracium species are scarce. ObjectiveEvaluation of the chemosystematic significance of flavonoids and phenolic acids of methanol extracts of aerial flowering parts of 28 Hieracium species from the Balkans. Additionally, investigation of antioxidant potentials of the extracts. MethodsComparative qualitative and quantitative analysis of flavonoids and phenolic acids was performed by LC-MS. Multivariate statistical data analysis included non-metric multidimensional scaling (nMDS), unweighted pair-group arithmetic averages (UPGMA) and principal component analysis (PCA). Antioxidant activity was evaluated using three colorimetric tests. ResultsDominant phenolics in almost all species were luteolin type flavonoids, followed by phenolic acids. Although the investigated Hieracium species share many compounds, the current classification of the genus was supported by nMDS and UPGMA analyses with a good resolution to the group level. Hieracium naegelianum was clearly separated from the other investigated species. Spatial and ecological distances of the samples were likely to influence unexpected differentiation of some groups within H. sect. Pannosa. The vast majority of dominant compounds significantly contributed to differences between taxa. The antioxidant potential of the extracts was satisfactory and in accordance with their phenolics composition. ConclusionsComparative LC-MS analysis demonstrated that flavonoids and phenolic acids are good indicators of chemosystematic relationships within Hieracium, particularly between non-hybrid species and groups from the same location. Copyright",
publisher = "Wiley, Hoboken",
journal = "Phytochemical Analysis",
title = "Methanol Extracts of 28 Hieracium Species from the Balkan Peninsula - Comparative LC-MS Analysis, Chemosystematic Evaluation of their Flavonoid and Phenolic Acid Profiles and Antioxidant Potentials",
volume = "29",
number = "1",
pages = "30-47",
doi = "10.1002/pca.2712"
}

Milutinović, V., Niketić, M., Ušjak, L., Nikolić, D., Krunić, A., Zidorn, C.,& Petrović, S.. (2018). Methanol Extracts of 28 Hieracium Species from the Balkan Peninsula - Comparative LC-MS Analysis, Chemosystematic Evaluation of their Flavonoid and Phenolic Acid Profiles and Antioxidant Potentials. in Phytochemical Analysis
Wiley, Hoboken., 29(1), 30-47.
https://doi.org/10.1002/pca.2712

Milutinović V, Niketić M, Ušjak L, Nikolić D, Krunić A, Zidorn C, Petrović S. Methanol Extracts of 28 Hieracium Species from the Balkan Peninsula - Comparative LC-MS Analysis, Chemosystematic Evaluation of their Flavonoid and Phenolic Acid Profiles and Antioxidant Potentials. in Phytochemical Analysis. 2018;29(1):30-47.
doi:10.1002/pca.2712 .

Milutinović, Violeta, Niketić, Marjan, Ušjak, Ljuboš, Nikolić, Dejan, Krunić, Aleksej, Zidorn, Christian, Petrović, Silvana, "Methanol Extracts of 28 Hieracium Species from the Balkan Peninsula - Comparative LC-MS Analysis, Chemosystematic Evaluation of their Flavonoid and Phenolic Acid Profiles and Antioxidant Potentials" in Phytochemical Analysis, 29, no. 1 (2018):30-47,
https://doi.org/10.1002/pca.2712 . .