@article{
author = "Milenković, Anđela and Miljić, Dragana and Mitrović, Jelena and Protić, Ana and Otašević, Biljana",
year = "2016",
abstract = "In recent years, there is aim to reduce toxic solvents use in chromatographic techniques. Beta-cyclodextrins (β-CD) are commonly used for this purpose, as substance retention modulators. By increasing their concentration in the mobile phase, generally there is a decrease in analyte retention time, and consequently, a decrease in the organic solvent ratio in the mobile phase. The aim of this study was to investigate β-CD and selected sartans inclusion complexes formation. The emphasis was on the influence of the aqueous phase pH value, the drug-β-CD molar ratio and the organic solvent type on complex formation. The mass spectrometry investigation included characterization of the formed complex drug: β-CD at different molar ratios (1:1, 1:2, 1:5 and 1:10), and at different pH values of the aqueous phase (5, 7 and 9) using triple quadrupole mass analyzer. The mobile phase and solvent in which the complexes were formed consisted of acetonitrile and water mixture (50:50, v/v) and methanol and water mixture (50:50, v/v). Measurements of these complexes signal intensity after electrospray ionization in negative mode. Observed m/z values indicated formation of drug-β-CD in 1:1 ratio at all pH values of water phase and independently of organic solvent type and β-CD ratio. It was noted that formed complex signal intensity increased only by increasing β-CD ratio., Poslednjih godina sve više se teži smanjenju upotrebe toksičnih rastvarača u hromatografskim metodama. U te svrhe često se upotrebljavaju beta-ciklodekstrini (β-CD) kao modulatori retencije supstanci. Povećavanjem njihove koncentracije u mobilnoj fazi uglavnom dolazi do smanjenja retencije analita i, posledično, do smanjenja udela organskog rastvarača u mobilnoj fazi. Cilj rada bio je ispitivanje načina formiranja inkluzionih kompleksa β-CD i odabranih lekova iz grupe sartana, sa posebnim osvrtom na uticaj pH vrednosti vodene faze, molarnog odnosa leka i β-CD i vrste organskog rastvarača na formiranje kompleksa. Primenom metode masene spektrometrije, na triplkvadrupolskom masenom analizatoru, vršena je karakterizacija formiranog kompleksa β-CD i leka pri različitom molarnom odnosu lek:β-CD (1:1, 1:2, 1:5 i 1:10) i pri pH vrednostima vodene faze 5, 7 i 9. Mobilna faza i rastvarač u kome su formirani kompleksi bila je smeša acetonitrila i vode, kao i smeša metanola i vode (50:50, V/V). Merenja intenziteta signala nagrađenih kompleksa nakon elektrosprej jonizacije vršena su u negativnom modu. Uočene su m/z vrednosti koje potiču od masa kompleksa leka i β-CD u odnosu 1:1 pri svim pH vrednostima vodene faze i nezavisno od vrste organskog rastvarača i udela β-CD. Uočeno je da intenzitet signala nastalog kompleksa raste jedino povećanjem udela β-CD.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Monitoring of complex forming of the active pharmaceutical substance and β-cyclodextrin as an additive of the mobile phase using mass spectrometry, Praćenje građenja kompleksa aktivne farmaceutske supstance i β-ciklodekstrina kao aditiva mobilne faze primenom masene spektrometrije",
volume = "66",
number = "4",
pages = "147-160",
doi = "10.5937/arhfarm1604147M"
}
