TY  - CONF
AU  - Martić, Radava
AU  - Krajišnik, Danina
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Savić, Snežana
AU  - Milić, Jela
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5247
AB  - INTRODUCTION
The SWOP (SWitch-Oil-Phase) emulsions are oil-in-water emulsions which are characterized by fast inversion into water-in-oil emulsions during application on the skin and consequent formation of a water-resistance layer over the skin [1]. Flavonoids quercetin (QUE) and dihydroquercetin (DHQ), as well as β-carotene (βC) are used in cosmetics as antioxidants. Additionally, these compounds show protective effects against ultraviolet radiation. Therefore, their incorporation into SWOP emulsion could result in a new waterproof sun protection product. The aim of this study was to prepare SWOP emulsion with 0.5% QUE (SQUE), 0.5% DHQ (SDHQ) and with combination of 0.5% DHQ and 0.2% βC (SDHQβC), and to evaluate DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability of incorporated antioxidants in comparison to the pure compounds.
MATERIALS AND METHODS
For this purpose, in vitro colorimetric DPPH assay was used [2, 3]. Results were expressed as the concentrations of antioxidants that scavenged 50% DPPH radicals, and analysed by one-way ANOVA, followed by Tukey’s post hoc test (α=0.05).
RESULTS
QUE and DHQ incorporated into SWOP emulsion exhibited strong anti-DPPH activity, without significant statistical difference compared to the pure compounds. The SC50 values of incorporated and pure QUE were 3.48±0.10 and 3.37±0.03 µg/mL, respectively. The SC50 values of DHQ incorporated in SDHQ and SDHQβC, and of pure DHQ were 5.36±0.27, 5.06±0.14 and 5.02±0.10 µg/mL, respectively. Neither incorporated nor pure βC showed anti-DPPH activity at tested concentrations (0.40-8.00 µg/mL). The SC50 values of tested SWOP emulsions, i.e. SQUE, SDHQ and SDHQβC were 0.70±0.02, 1.07±0.05, 1.01±0.03 mg/mL, respectively.
CONCLUSION
QUE and DHQ incorporated into SWOP emulsion retain their strong anti-DPPH activity, i.e. investigated SWOP emulsion is a suitable vehicle/base for the tested flavonoids. On the other hand, although known for its antioxidant activity, βC showed no anti-DPPH activity, which is in agreement with findings of some other authors [4]. Therefore, DPPH assay is not suitable for the testing of antioxidant activity of βC incorporated into SWOP emulsion.
REFERENCES
1.	Beuché M. et al. EP1917954 A1 (2008)
2.	Cuendet M. et al. Helv Chim Acta. 80, 1144-52 (1997)
3.	Casagrande R. et al. Int J Pharm. 328, 183–190 (2007)
4.	Müller L. et al. Food Chem. 129, 139-48 (2011)
PB  - Hungarian Society for Pharmaceutical Sciences
C3  - Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
T1  - Investigation of DPPH radical scavenging ability of different antioxidants incorporated into fast inverted oil-in-water emulsion
VL  - 88
IS  - 043
SP  - 159
EP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5247
ER  - 

@conference{
author = "Martić, Radava and Krajišnik, Danina and Ušjak, Ljuboš and Petrović, Silvana and Savić, Snežana and Milić, Jela",
year = "2018",
abstract = "INTRODUCTION
The SWOP (SWitch-Oil-Phase) emulsions are oil-in-water emulsions which are characterized by fast inversion into water-in-oil emulsions during application on the skin and consequent formation of a water-resistance layer over the skin [1]. Flavonoids quercetin (QUE) and dihydroquercetin (DHQ), as well as β-carotene (βC) are used in cosmetics as antioxidants. Additionally, these compounds show protective effects against ultraviolet radiation. Therefore, their incorporation into SWOP emulsion could result in a new waterproof sun protection product. The aim of this study was to prepare SWOP emulsion with 0.5% QUE (SQUE), 0.5% DHQ (SDHQ) and with combination of 0.5% DHQ and 0.2% βC (SDHQβC), and to evaluate DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability of incorporated antioxidants in comparison to the pure compounds.
MATERIALS AND METHODS
For this purpose, in vitro colorimetric DPPH assay was used [2, 3]. Results were expressed as the concentrations of antioxidants that scavenged 50% DPPH radicals, and analysed by one-way ANOVA, followed by Tukey’s post hoc test (α=0.05).
RESULTS
QUE and DHQ incorporated into SWOP emulsion exhibited strong anti-DPPH activity, without significant statistical difference compared to the pure compounds. The SC50 values of incorporated and pure QUE were 3.48±0.10 and 3.37±0.03 µg/mL, respectively. The SC50 values of DHQ incorporated in SDHQ and SDHQβC, and of pure DHQ were 5.36±0.27, 5.06±0.14 and 5.02±0.10 µg/mL, respectively. Neither incorporated nor pure βC showed anti-DPPH activity at tested concentrations (0.40-8.00 µg/mL). The SC50 values of tested SWOP emulsions, i.e. SQUE, SDHQ and SDHQβC were 0.70±0.02, 1.07±0.05, 1.01±0.03 mg/mL, respectively.
CONCLUSION
QUE and DHQ incorporated into SWOP emulsion retain their strong anti-DPPH activity, i.e. investigated SWOP emulsion is a suitable vehicle/base for the tested flavonoids. On the other hand, although known for its antioxidant activity, βC showed no anti-DPPH activity, which is in agreement with findings of some other authors [4]. Therefore, DPPH assay is not suitable for the testing of antioxidant activity of βC incorporated into SWOP emulsion.
REFERENCES
1.	Beuché M. et al. EP1917954 A1 (2008)
2.	Cuendet M. et al. Helv Chim Acta. 80, 1144-52 (1997)
3.	Casagrande R. et al. Int J Pharm. 328, 183–190 (2007)
4.	Müller L. et al. Food Chem. 129, 139-48 (2011)",
publisher = "Hungarian Society for Pharmaceutical Sciences",
journal = "Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts",
title = "Investigation of DPPH radical scavenging ability of different antioxidants incorporated into fast inverted oil-in-water emulsion",
volume = "88",
number = "043",
pages = "159-160",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5247"
}

Martić, R., Krajišnik, D., Ušjak, L., Petrović, S., Savić, S.,& Milić, J.. (2018). Investigation of DPPH radical scavenging ability of different antioxidants incorporated into fast inverted oil-in-water emulsion. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
Hungarian Society for Pharmaceutical Sciences., 88(043), 159-160.
https://hdl.handle.net/21.15107/rcub_farfar_5247

Martić R, Krajišnik D, Ušjak L, Petrović S, Savić S, Milić J. Investigation of DPPH radical scavenging ability of different antioxidants incorporated into fast inverted oil-in-water emulsion. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts. 2018;88(043):159-160.
https://hdl.handle.net/21.15107/rcub_farfar_5247 .

Martić, Radava, Krajišnik, Danina, Ušjak, Ljuboš, Petrović, Silvana, Savić, Snežana, Milić, Jela, "Investigation of DPPH radical scavenging ability of different antioxidants incorporated into fast inverted oil-in-water emulsion" in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts, 88, no. 043 (2018):159-160,
https://hdl.handle.net/21.15107/rcub_farfar_5247 .