TY  - JOUR
AU  - Toljić, Mina
AU  - Egić, Amira
AU  - Munjas, Jelena
AU  - Orlić, Nataša Karadzov
AU  - Milovanović, Zagorka
AU  - Radenković, Aleksandra
AU  - Vuceljić, Jovana
AU  - Joksić, Ivana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2808
AB  - We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Research in Microbiology
T1  - Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension
VL  - 71
SP  - 55
EP  - 62
DO  - 10.1016/j.reprotox.2017.04.002
ER  - 

@article{
author = "Toljić, Mina and Egić, Amira and Munjas, Jelena and Orlić, Nataša Karadzov and Milovanović, Zagorka and Radenković, Aleksandra and Vuceljić, Jovana and Joksić, Ivana",
year = "2017",
abstract = "We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Research in Microbiology",
title = "Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension",
volume = "71",
pages = "55-62",
doi = "10.1016/j.reprotox.2017.04.002"
}

Toljić, M., Egić, A., Munjas, J., Orlić, N. K., Milovanović, Z., Radenković, A., Vuceljić, J.,& Joksić, I.. (2017). Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension. in Research in Microbiology
Pergamon-Elsevier Science Ltd, Oxford., 71, 55-62.
https://doi.org/10.1016/j.reprotox.2017.04.002

Toljić M, Egić A, Munjas J, Orlić NK, Milovanović Z, Radenković A, Vuceljić J, Joksić I. Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension. in Research in Microbiology. 2017;71:55-62.
doi:10.1016/j.reprotox.2017.04.002 .

Toljić, Mina, Egić, Amira, Munjas, Jelena, Orlić, Nataša Karadzov, Milovanović, Zagorka, Radenković, Aleksandra, Vuceljić, Jovana, Joksić, Ivana, "Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension" in Research in Microbiology, 71 (2017):55-62,
https://doi.org/10.1016/j.reprotox.2017.04.002 . .