TY  - JOUR
AU  - Joksić, Gordana
AU  - Micić, Mileva
AU  - Filipović, Jelena
AU  - Drakulić, Dunja
AU  - Stanojlović, Miloš
AU  - Čalija, Bojan
AU  - Valenta Sobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3015
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Micić, Mileva and Filipović, Jelena and Drakulić, Dunja and Stanojlović, Miloš and Čalija, Bojan and Valenta Sobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Micić, M., Filipović, J., Drakulić, D., Stanojlović, M., Čalija, B., Valenta Sobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Micić M, Filipović J, Drakulić D, Stanojlović M, Čalija B, Valenta Sobot A, Demajo M, Nilsson R. Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Micić, Mileva, Filipović, Jelena, Drakulić, Dunja, Stanojlović, Miloš, Čalija, Bojan, Valenta Sobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach" in Acta veterinaria, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .