TY  - JOUR
AU  - Stojanović, Danilo
AU  - Aleksić, Jelena M.
AU  - Jančić, Ivan
AU  - Jančić, Radiša
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2376
AB  - Salvia officinalis (Lamiaceae), common or Dalmatian sage, is a Mediterranean aromatic and medicinal plant used in medicine since ancient times. Knowledge on current genetic patterns and genealogical history of its natural populations is required for both breeding efforts and species conservation. We used sequences of two chloroplast intergenic spacers, 3'rps16-5'trnK and rp132-trnL, from 83 individuals from eight natural populations to distinguish between anthropogenic vs natural origin of four disjunct inland populations found outside of the main Adriatic range of the species. We found seven haplotypes, high total gene diversity (H-r = 0.695) and genetic differentiation (G(ST) = 0.682), as well as a phylogeographic structure with two lineages, a sub-structured inland-Adriatic lineage (IAL, comprising inland and Adriatic sub-lineages) and a purely Adriatic lineage (PAL). All four inland and disjunct populations, which comprised the inland sub-lineage of IAL, were almost fixed for a distinct haplotype genealogically closely related to the ancestral haplotype and displayed other features of relict populations. Along with previous biogeographic data and other lines of evidence, assumptions on their anthropogenic origin were rejected. At present, a less diverse IAL (Hd = 0.426, pi = 0.00106) and a more diverse PAL (Hd = 0.403, pi = 0.00257), whose divergence was dated to the Pliocene (3.267 Mya), do not exhibit signs of recent demographic expansions and overlap on the SE Adriatic coast, a region delineated as the main glacial refugium of S. officinalis. Conservation measures accounting for the historical distinctiveness of populations and focusing on currently the most threatened populations are recommended.
PB  - Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin
T2  - Women & Health
T1  - A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications
VL  - 45
IS  - 1
SP  - 103
EP  - 118
DO  - 10.3372/wi.45.45112
ER  - 

@article{
author = "Stojanović, Danilo and Aleksić, Jelena M. and Jančić, Ivan and Jančić, Radiša",
year = "2015",
abstract = "Salvia officinalis (Lamiaceae), common or Dalmatian sage, is a Mediterranean aromatic and medicinal plant used in medicine since ancient times. Knowledge on current genetic patterns and genealogical history of its natural populations is required for both breeding efforts and species conservation. We used sequences of two chloroplast intergenic spacers, 3'rps16-5'trnK and rp132-trnL, from 83 individuals from eight natural populations to distinguish between anthropogenic vs natural origin of four disjunct inland populations found outside of the main Adriatic range of the species. We found seven haplotypes, high total gene diversity (H-r = 0.695) and genetic differentiation (G(ST) = 0.682), as well as a phylogeographic structure with two lineages, a sub-structured inland-Adriatic lineage (IAL, comprising inland and Adriatic sub-lineages) and a purely Adriatic lineage (PAL). All four inland and disjunct populations, which comprised the inland sub-lineage of IAL, were almost fixed for a distinct haplotype genealogically closely related to the ancestral haplotype and displayed other features of relict populations. Along with previous biogeographic data and other lines of evidence, assumptions on their anthropogenic origin were rejected. At present, a less diverse IAL (Hd = 0.426, pi = 0.00106) and a more diverse PAL (Hd = 0.403, pi = 0.00257), whose divergence was dated to the Pliocene (3.267 Mya), do not exhibit signs of recent demographic expansions and overlap on the SE Adriatic coast, a region delineated as the main glacial refugium of S. officinalis. Conservation measures accounting for the historical distinctiveness of populations and focusing on currently the most threatened populations are recommended.",
publisher = "Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin",
journal = "Women & Health",
title = "A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications",
volume = "45",
number = "1",
pages = "103-118",
doi = "10.3372/wi.45.45112"
}

Stojanović, D., Aleksić, J. M., Jančić, I.,& Jančić, R.. (2015). A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications. in Women & Health
Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin., 45(1), 103-118.
https://doi.org/10.3372/wi.45.45112

Stojanović D, Aleksić JM, Jančić I, Jančić R. A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications. in Women & Health. 2015;45(1):103-118.
doi:10.3372/wi.45.45112 .

Stojanović, Danilo, Aleksić, Jelena M., Jančić, Ivan, Jančić, Radiša, "A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications" in Women & Health, 45, no. 1 (2015):103-118,
https://doi.org/10.3372/wi.45.45112 . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Stojanović, Danilo
AU  - Banović, Bojana
AU  - Jančić, Radiša
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1675
AB  - We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - A Simple and Efficient DNA Isolation Method for Salvia officinalis
VL  - 50
IS  - 11-12
SP  - 881
EP  - 892
DO  - 10.1007/s10528-012-9528-y
ER  - 

@article{
author = "Aleksić, Jelena M. and Stojanović, Danilo and Banović, Bojana and Jančić, Radiša",
year = "2012",
abstract = "We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "A Simple and Efficient DNA Isolation Method for Salvia officinalis",
volume = "50",
number = "11-12",
pages = "881-892",
doi = "10.1007/s10528-012-9528-y"
}

Aleksić, J. M., Stojanović, D., Banović, B.,& Jančić, R.. (2012). A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics
Springer/Plenum Publishers, New York., 50(11-12), 881-892.
https://doi.org/10.1007/s10528-012-9528-y

Aleksić JM, Stojanović D, Banović B, Jančić R. A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics. 2012;50(11-12):881-892.
doi:10.1007/s10528-012-9528-y .

Aleksić, Jelena M., Stojanović, Danilo, Banović, Bojana, Jančić, Radiša, "A Simple and Efficient DNA Isolation Method for Salvia officinalis" in Biochemical Genetics, 50, no. 11-12 (2012):881-892,
https://doi.org/10.1007/s10528-012-9528-y . .