TY  - JOUR
AU  - Odović, Jadranka
AU  - Marković, Bojan
AU  - Injac, Rade
AU  - Vladimirov, Sote
AU  - Karljiković-Rajić, Katarina
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1651
AB  - In this research seven ACE inhibitors (enalapril, quinapril, fosinopril, lisinopril, cilazapril, ramipril, benazepril) were studied to evaluate the correlation between their absorption and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS) and reversed-phase thin-layer chromatography (RP-TLC) hydrophobicity data (phi(0) or C-0 parameters, respectively). Their absorption values were in the range of 25-60%, while calculated KOWWIN logP values were from -0.94 to 6.61. Additionally. perindopril (absorption 70%, KOWWIN logP 2.59) and moexipril (absorption 22%, KOWWIN logP 3.36) were introduced for the theoretical considerations due to their high/low absorption values which were on the opposite sites in comparison with the majority of ACE inhibitors (25-60%). In the theoretical considerations it was shown that the solubility data (logS) must be considered, as independent variable, simultaneously with KOWWIN logP to obtain reliable correlation (r(2) = 0.7208) between absorption and ACE inhibitors lipophilicity. As the main topic of this study, the relationships between literature available and absorption data predicted by multiple linear regression (MLR) using logS values besides chromatographically obtained hydrophobicity parameters C-0 (r(2) = 0.6424) or phi(0) (r(2) = 0.6762) were studied proving that these parameters could be used in ACE inhibitors absorption evaluation. The UHPLC-MS method provides the direct application of experimentally obtained phi(0) values that is the advantage of this method. For better MLR correlation of ACE inhibitors absorption with C-0 parameters (RP-TLC) and logS, mathematical conversion of C-0 parameters to logC(0) values was necessary based on requisite for probability value of regression analysis (P  lt  0.05). The accordance and differences between hydrophobicity parameters obtained by UHPLC-MS and RP-TLC were defined.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography A
T1  - Correlation between ultra-high performance liquid chromatography-tandem mass spectrometry and reversed-phase thin-layer chromatography hydrophobicity data for evaluation of angiotensin-converting enzyme inhibitors absorption
VL  - 1258
SP  - 94
EP  - 100
DO  - 10.1016/j.chroma.2012.08.038
ER  - 

@article{
author = "Odović, Jadranka and Marković, Bojan and Injac, Rade and Vladimirov, Sote and Karljiković-Rajić, Katarina",
year = "2012",
abstract = "In this research seven ACE inhibitors (enalapril, quinapril, fosinopril, lisinopril, cilazapril, ramipril, benazepril) were studied to evaluate the correlation between their absorption and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS) and reversed-phase thin-layer chromatography (RP-TLC) hydrophobicity data (phi(0) or C-0 parameters, respectively). Their absorption values were in the range of 25-60%, while calculated KOWWIN logP values were from -0.94 to 6.61. Additionally. perindopril (absorption 70%, KOWWIN logP 2.59) and moexipril (absorption 22%, KOWWIN logP 3.36) were introduced for the theoretical considerations due to their high/low absorption values which were on the opposite sites in comparison with the majority of ACE inhibitors (25-60%). In the theoretical considerations it was shown that the solubility data (logS) must be considered, as independent variable, simultaneously with KOWWIN logP to obtain reliable correlation (r(2) = 0.7208) between absorption and ACE inhibitors lipophilicity. As the main topic of this study, the relationships between literature available and absorption data predicted by multiple linear regression (MLR) using logS values besides chromatographically obtained hydrophobicity parameters C-0 (r(2) = 0.6424) or phi(0) (r(2) = 0.6762) were studied proving that these parameters could be used in ACE inhibitors absorption evaluation. The UHPLC-MS method provides the direct application of experimentally obtained phi(0) values that is the advantage of this method. For better MLR correlation of ACE inhibitors absorption with C-0 parameters (RP-TLC) and logS, mathematical conversion of C-0 parameters to logC(0) values was necessary based on requisite for probability value of regression analysis (P  lt  0.05). The accordance and differences between hydrophobicity parameters obtained by UHPLC-MS and RP-TLC were defined.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography A",
title = "Correlation between ultra-high performance liquid chromatography-tandem mass spectrometry and reversed-phase thin-layer chromatography hydrophobicity data for evaluation of angiotensin-converting enzyme inhibitors absorption",
volume = "1258",
pages = "94-100",
doi = "10.1016/j.chroma.2012.08.038"
}

Odović, J., Marković, B., Injac, R., Vladimirov, S.,& Karljiković-Rajić, K.. (2012). Correlation between ultra-high performance liquid chromatography-tandem mass spectrometry and reversed-phase thin-layer chromatography hydrophobicity data for evaluation of angiotensin-converting enzyme inhibitors absorption. in Journal of Chromatography A
Elsevier Science BV, Amsterdam., 1258, 94-100.
https://doi.org/10.1016/j.chroma.2012.08.038

Odović J, Marković B, Injac R, Vladimirov S, Karljiković-Rajić K. Correlation between ultra-high performance liquid chromatography-tandem mass spectrometry and reversed-phase thin-layer chromatography hydrophobicity data for evaluation of angiotensin-converting enzyme inhibitors absorption. in Journal of Chromatography A. 2012;1258:94-100.
doi:10.1016/j.chroma.2012.08.038 .

Odović, Jadranka, Marković, Bojan, Injac, Rade, Vladimirov, Sote, Karljiković-Rajić, Katarina, "Correlation between ultra-high performance liquid chromatography-tandem mass spectrometry and reversed-phase thin-layer chromatography hydrophobicity data for evaluation of angiotensin-converting enzyme inhibitors absorption" in Journal of Chromatography A, 1258 (2012):94-100,
https://doi.org/10.1016/j.chroma.2012.08.038 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Mlinarić, Ales
AU  - Đorđević-Milić, Vukosava
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1103
AB  - A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
T1  - Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography
VL  - 25
IS  - 4
SP  - 424
EP  - 431
DO  - 10.1080/02652030701584058
ER  - 

@article{
author = "Injac, Rade and Mlinarić, Ales and Đorđević-Milić, Vukosava and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment",
title = "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography",
volume = "25",
number = "4",
pages = "424-431",
doi = "10.1080/02652030701584058"
}

Injac, R., Mlinarić, A., Đorđević-Milić, V., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
Taylor & Francis Ltd, Abingdon., 25(4), 424-431.
https://doi.org/10.1080/02652030701584058

Injac R, Mlinarić A, Đorđević-Milić V, Karljiković-Rajić K, Štrukelj B. Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment. 2008;25(4):424-431.
doi:10.1080/02652030701584058 .

Injac, Rade, Mlinarić, Ales, Đorđević-Milić, Vukosava, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography" in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 25, no. 4 (2008):424-431,
https://doi.org/10.1080/02652030701584058 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Srđenović, Branislava
AU  - Prijatelj, Matevz
AU  - Bošković, Marija
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1053
PB  - Preston Publ Inc, Niles
T2  - Journal of Chromatographic Science
T1  - Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography
VL  - 46
IS  - 2
SP  - 137
EP  - 143
DO  - 10.1093/chromsci/46.2.137
ER  - 

@article{
author = "Injac, Rade and Srđenović, Branislava and Prijatelj, Matevz and Bošković, Marija and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
publisher = "Preston Publ Inc, Niles",
journal = "Journal of Chromatographic Science",
title = "Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography",
volume = "46",
number = "2",
pages = "137-143",
doi = "10.1093/chromsci/46.2.137"
}

Injac, R., Srđenović, B., Prijatelj, M., Bošković, M., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography. in Journal of Chromatographic Science
Preston Publ Inc, Niles., 46(2), 137-143.
https://doi.org/10.1093/chromsci/46.2.137

Injac R, Srđenović B, Prijatelj M, Bošković M, Karljiković-Rajić K, Štrukelj B. Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography. in Journal of Chromatographic Science. 2008;46(2):137-143.
doi:10.1093/chromsci/46.2.137 .

Injac, Rade, Srđenović, Branislava, Prijatelj, Matevz, Bošković, Marija, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography" in Journal of Chromatographic Science, 46, no. 2 (2008):137-143,
https://doi.org/10.1093/chromsci/46.2.137 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1134
AB  - Micellar electrokinetic capillary chromatography (MEKC) has become a popular mode among the several capillary electro-migration techniques. Most drug analysis can be performed by using MEKC because of its wide applicability. Separation of very complex mixtures, determination of drugs in the biological materials, etc., can be successfully achieved by MEKC. This review surveys typical applications of MEKC analysis. Recent advances in MEKC, especially with solid-phase extraction and large-volume sample stacking, are described. Modes of electrokinetic chromatography including MEKC, a separation theory of MEKC, environmental friendly analysis, and selectivity manipulation in MEKC are also briefly mentioned.
AB  - Micelama elektrokinetička kapilarna hromatografija (MEKC) postala je najpopularnija kapilarna elektromigraciona metoda. Na osnovu veoma široke upotrebe metode, većina analiza lekova moguća je uz MEKC. Separacija veoma kompleksnih smeša, određivanje lekova u biološkom materijalu, i analize drugih uzoraka, mogu biti veoma uspešno izvedene upotrebom MEKC tehnike. U okviru ovog preglednog rada prikazana je aplikacija tipičnih MEKC metoda. Noviji pristupi u kombinaciji sa čvrsto-faznom ekstrakcijom i visoko-volumskim injektovanjem su takođe opisani. Teorijski i ekološki pristup, kao i prednosti i nedostatci same metode, prikazani su teorijski i kroz praktične primere.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Application of micellar electrokinetic capillary chromatography for routine analysis of different materials
T1  - Upotreba micelarne elektrokinetičke kapilarne hromatografije u rutinskoj analizi različitih uzoraka
VL  - 62
IS  - 3
SP  - 181
EP  - 190
DO  - 10.2298/HEMIND0803181I
ER  - 

@article{
author = "Injac, Rade and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "Micellar electrokinetic capillary chromatography (MEKC) has become a popular mode among the several capillary electro-migration techniques. Most drug analysis can be performed by using MEKC because of its wide applicability. Separation of very complex mixtures, determination of drugs in the biological materials, etc., can be successfully achieved by MEKC. This review surveys typical applications of MEKC analysis. Recent advances in MEKC, especially with solid-phase extraction and large-volume sample stacking, are described. Modes of electrokinetic chromatography including MEKC, a separation theory of MEKC, environmental friendly analysis, and selectivity manipulation in MEKC are also briefly mentioned., Micelama elektrokinetička kapilarna hromatografija (MEKC) postala je najpopularnija kapilarna elektromigraciona metoda. Na osnovu veoma široke upotrebe metode, većina analiza lekova moguća je uz MEKC. Separacija veoma kompleksnih smeša, određivanje lekova u biološkom materijalu, i analize drugih uzoraka, mogu biti veoma uspešno izvedene upotrebom MEKC tehnike. U okviru ovog preglednog rada prikazana je aplikacija tipičnih MEKC metoda. Noviji pristupi u kombinaciji sa čvrsto-faznom ekstrakcijom i visoko-volumskim injektovanjem su takođe opisani. Teorijski i ekološki pristup, kao i prednosti i nedostatci same metode, prikazani su teorijski i kroz praktične primere.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Application of micellar electrokinetic capillary chromatography for routine analysis of different materials, Upotreba micelarne elektrokinetičke kapilarne hromatografije u rutinskoj analizi različitih uzoraka",
volume = "62",
number = "3",
pages = "181-190",
doi = "10.2298/HEMIND0803181I"
}

Injac, R., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Application of micellar electrokinetic capillary chromatography for routine analysis of different materials. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 62(3), 181-190.
https://doi.org/10.2298/HEMIND0803181I

Injac R, Karljiković-Rajić K, Štrukelj B. Application of micellar electrokinetic capillary chromatography for routine analysis of different materials. in Hemijska industrija. 2008;62(3):181-190.
doi:10.2298/HEMIND0803181I .

Injac, Rade, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Application of micellar electrokinetic capillary chromatography for routine analysis of different materials" in Hemijska industrija, 62, no. 3 (2008):181-190,
https://doi.org/10.2298/HEMIND0803181I . .

TY  - JOUR
AU  - Injac, Rade
AU  - Kac, Javor
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1110
AB  - A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.
PB  - Food & Drug Adminstration, Taipei
T2  - Journal of Food and Drug Analysis
T1  - Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography
VL  - 16
IS  - 1
SP  - 18
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1110
ER  - 

@article{
author = "Injac, Rade and Kac, Javor and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.",
publisher = "Food & Drug Adminstration, Taipei",
journal = "Journal of Food and Drug Analysis",
title = "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography",
volume = "16",
number = "1",
pages = "18-25",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1110"
}

Injac, R., Kac, J., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. in Journal of Food and Drug Analysis
Food & Drug Adminstration, Taipei., 16(1), 18-25.
https://hdl.handle.net/21.15107/rcub_farfar_1110

Injac R, Kac J, Karljiković-Rajić K, Štrukelj B. Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. in Journal of Food and Drug Analysis. 2008;16(1):18-25.
https://hdl.handle.net/21.15107/rcub_farfar_1110 .

Injac, Rade, Kac, Javor, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography" in Journal of Food and Drug Analysis, 16, no. 1 (2008):18-25,
https://hdl.handle.net/21.15107/rcub_farfar_1110 .

TY  - JOUR
AU  - Injac, Rade
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1061
AB  - A novel and simple method has been developed for the determination of doxycycline (DOX) in biological fluids. The method is based on SPE, large-volume sample stacking (LVSS) and MEKC with UV-DAD detection. Six SPE cartridges have been used in investigation for sample clean up and pre-concentration (Supelco (R) LC-8, LC-18, LC-SCX, and LC-WCX, as well as Strata (TM)-X and X-C). DOX was determined on a 56 cm. (effective length 50 cm) x 50 mu m id fused-silica capillary. The BGE was 20 mM borate buffer, pH 9.3, containing 80 mM SDS and 7.5% v/v of methanol (30 s x 50 mbar), and the temperature and voltage were 25 degrees C and 30 kV, respectively. The analytical wavelength was set at 210 nm. Under optimized conditions it is possible to determine DOX in human serum, urine, semen, tears and saliva with recovery of 97.5% (RSD 2.5%). The method was shown to be sensitive (LOD is 1 mu g/L) and precise (intra-day RSD 0.2 and 2.4%; inter-days 0.4 and 3.5% for migration time and peak area, respectively). Results for developed SPE-LVSS-MEKC were compared with LVSS-MEKC method with direct sample injection. The new LVSS-MEKC method is presented as a useful technique for rapid determination without extraction procedure of DOX in human urine and serum, using 80mM of SDS, 10% v/v of methanol and 40 mM borate buffer (pH 9.3; 30 s x 50 mbar; 25 degrees C; 30 kV; 350 nm), but not for the other biological fluids, according to lower sensitivity of the method and because of the sample composition.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Electrophoresis
T1  - SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC
VL  - 29
IS  - 21
SP  - 4431
EP  - 4438
DO  - 10.1002/elps.200800339
ER  - 

@article{
author = "Injac, Rade and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A novel and simple method has been developed for the determination of doxycycline (DOX) in biological fluids. The method is based on SPE, large-volume sample stacking (LVSS) and MEKC with UV-DAD detection. Six SPE cartridges have been used in investigation for sample clean up and pre-concentration (Supelco (R) LC-8, LC-18, LC-SCX, and LC-WCX, as well as Strata (TM)-X and X-C). DOX was determined on a 56 cm. (effective length 50 cm) x 50 mu m id fused-silica capillary. The BGE was 20 mM borate buffer, pH 9.3, containing 80 mM SDS and 7.5% v/v of methanol (30 s x 50 mbar), and the temperature and voltage were 25 degrees C and 30 kV, respectively. The analytical wavelength was set at 210 nm. Under optimized conditions it is possible to determine DOX in human serum, urine, semen, tears and saliva with recovery of 97.5% (RSD 2.5%). The method was shown to be sensitive (LOD is 1 mu g/L) and precise (intra-day RSD 0.2 and 2.4%; inter-days 0.4 and 3.5% for migration time and peak area, respectively). Results for developed SPE-LVSS-MEKC were compared with LVSS-MEKC method with direct sample injection. The new LVSS-MEKC method is presented as a useful technique for rapid determination without extraction procedure of DOX in human urine and serum, using 80mM of SDS, 10% v/v of methanol and 40 mM borate buffer (pH 9.3; 30 s x 50 mbar; 25 degrees C; 30 kV; 350 nm), but not for the other biological fluids, according to lower sensitivity of the method and because of the sample composition.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Electrophoresis",
title = "SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC",
volume = "29",
number = "21",
pages = "4431-4438",
doi = "10.1002/elps.200800339"
}

Injac, R., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC. in Electrophoresis
Wiley-VCH Verlag GMBH, Weinheim., 29(21), 4431-4438.
https://doi.org/10.1002/elps.200800339

Injac R, Karljiković-Rajić K, Štrukelj B. SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC. in Electrophoresis. 2008;29(21):4431-4438.
doi:10.1002/elps.200800339 .

Injac, Rade, Karljiković-Rajić, Katarina, Štrukelj, Borut, "SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC" in Electrophoresis, 29, no. 21 (2008):4431-4438,
https://doi.org/10.1002/elps.200800339 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Kac, Javor
AU  - Mlinarić, Ales
AU  - Karljiković-Rajić, Katarina
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/825
AB  - An MEKC procedure was developed for the separation of zinc bacitracin (Zn-BC) and nystatin (NYS) in mixtures and in animal feedstuff. The running buffer was 15 mM borate/19 mM phosphate, pH 8.2, containing 20 mM SDS and 10% v/v methanol. Samples were run at 25 degrees C, the applied voltage was 25 IN, and an additional pressure of 5 mbar was applied. Both analytes were detected by LTV simultaneously at 215 nm, Zn-BC alone at 192 and 254 nm, and NYS alone at 305 nm. The method was shown to be specific, accurate (recoveries were 100.0 +/- 0.6% and 100.1 +/- 0.6% for Zn-BC and NYS, respectively), linear over the tested range (correlation coefficients 0.9991 and 0.9994), and precise (RSD below 1.3% for both analytes). The method was applied to determine Zn-BC and NYS as additives in animal feed.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Journal of Separation Science
T1  - Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed
VL  - 29
IS  - 9
SP  - 1288
EP  - 1293
DO  - 10.1002/jssc.200500477
ER  - 

@article{
author = "Injac, Rade and Kac, Javor and Mlinarić, Ales and Karljiković-Rajić, Katarina",
year = "2006",
abstract = "An MEKC procedure was developed for the separation of zinc bacitracin (Zn-BC) and nystatin (NYS) in mixtures and in animal feedstuff. The running buffer was 15 mM borate/19 mM phosphate, pH 8.2, containing 20 mM SDS and 10% v/v methanol. Samples were run at 25 degrees C, the applied voltage was 25 IN, and an additional pressure of 5 mbar was applied. Both analytes were detected by LTV simultaneously at 215 nm, Zn-BC alone at 192 and 254 nm, and NYS alone at 305 nm. The method was shown to be specific, accurate (recoveries were 100.0 +/- 0.6% and 100.1 +/- 0.6% for Zn-BC and NYS, respectively), linear over the tested range (correlation coefficients 0.9991 and 0.9994), and precise (RSD below 1.3% for both analytes). The method was applied to determine Zn-BC and NYS as additives in animal feed.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Journal of Separation Science",
title = "Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed",
volume = "29",
number = "9",
pages = "1288-1293",
doi = "10.1002/jssc.200500477"
}

Injac, R., Kac, J., Mlinarić, A.,& Karljiković-Rajić, K.. (2006). Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed. in Journal of Separation Science
Wiley-VCH Verlag GMBH, Weinheim., 29(9), 1288-1293.
https://doi.org/10.1002/jssc.200500477

Injac R, Kac J, Mlinarić A, Karljiković-Rajić K. Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed. in Journal of Separation Science. 2006;29(9):1288-1293.
doi:10.1002/jssc.200500477 .

Injac, Rade, Kac, Javor, Mlinarić, Ales, Karljiković-Rajić, Katarina, "Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed" in Journal of Separation Science, 29, no. 9 (2006):1288-1293,
https://doi.org/10.1002/jssc.200500477 . .