TY  - JOUR
AU  - Joksić, Jelena
AU  - Sopić, Miron
AU  - Spasojević-Kalimanovska, Vesna
AU  - Gojković, Tamara
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Anđelković, Kristina
AU  - Kalimanovska-Oštrić, Dimitra
AU  - Jelić-Ivanović, Zorana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2575
AB  - Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles  lt  50%. Plasma resistin correlated positively with creatinine (r = 0.456, P  lt  0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.
PB  - Wiley, Hoboken
T2  - Clinical and Experimental Pharmacology and Physiology
T1  - Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients
VL  - 43
IS  - 1
SP  - 22
EP  - 28
DO  - 10.1111/1440-1681.12503
ER  - 

@article{
author = "Joksić, Jelena and Sopić, Miron and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Zeljković, Aleksandra and Vekić, Jelena and Anđelković, Kristina and Kalimanovska-Oštrić, Dimitra and Jelić-Ivanović, Zorana",
year = "2016",
abstract = "Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles  lt  50%. Plasma resistin correlated positively with creatinine (r = 0.456, P  lt  0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.",
publisher = "Wiley, Hoboken",
journal = "Clinical and Experimental Pharmacology and Physiology",
title = "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients",
volume = "43",
number = "1",
pages = "22-28",
doi = "10.1111/1440-1681.12503"
}

Joksić, J., Sopić, M., Spasojević-Kalimanovska, V., Gojković, T., Zeljković, A., Vekić, J., Anđelković, K., Kalimanovska-Oštrić, D.,& Jelić-Ivanović, Z.. (2016). Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology
Wiley, Hoboken., 43(1), 22-28.
https://doi.org/10.1111/1440-1681.12503

Joksić J, Sopić M, Spasojević-Kalimanovska V, Gojković T, Zeljković A, Vekić J, Anđelković K, Kalimanovska-Oštrić D, Jelić-Ivanović Z. Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology. 2016;43(1):22-28.
doi:10.1111/1440-1681.12503 .

Joksić, Jelena, Sopić, Miron, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Zeljković, Aleksandra, Vekić, Jelena, Anđelković, Kristina, Kalimanovska-Oštrić, Dimitra, Jelić-Ivanović, Zorana, "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients" in Clinical and Experimental Pharmacology and Physiology, 43, no. 1 (2016):22-28,
https://doi.org/10.1111/1440-1681.12503 . .

TY  - JOUR
AU  - Joksić, Jelena
AU  - Sopić, Miron
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kalimanovska-Oštrić, Dimitra
AU  - Anđelković, Kristina
AU  - Jelić-Ivanović, Zorana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2465
AB  - Introduction: Previous studies have implicated a strong link between circulating plasma resistin and coronary artery disease (CAD). The aim of this study was to evaluate the differences in peripheral blood mononuclear cells (PBMC) resistin mRNA and its plasma protein concentrations between the patients with CAD of different clinical severity. Material and methods: This study included 33 healthy subjects as the control group (CG) and 77 patients requiring coronary angiography. Of the latter 30 was CAD negative whereas 47 were CAD positive [18 with stable angina pectoris (SAP) and 29 with acute coronary syndrome (ACS)]. Circulating resistin was measured by ELISA; PBMC resistin mRNA was determined by real-time PCR. Results: Resistin protein was significantly higher in the ACS group compared to the CG (P = 0.001) and the CAD negative group (P = 0.018). Resistin mRNA expression did not vary across the study groups, despite the positive correlation seen with plasma resistin (rho = 0.305, P = 0.008). In patients, plasma resistin and PBMC resistin mRNA negatively correlated with HDL-C (rho = -0.404, P  lt  0.001 and rho = -0.257, P = 0.032, respectively). Furthermore, the highest plasma resistin tertile showed the lowest HDL-C (P = 0.006). Plasma resistin was positively associated with serum creatinine (rho = 0.353, P = 0.002). Conclusion: Significant increase of plasma resistin in patients with ACS compared to CG and CAD negative patients was observed. Despite no change in PBMC resistin mRNA in different disease conditions a positive association between resistin mRNA and resistin plasma protein was evident. Both plasma resistin and PBMC resistin mRNA were negatively associated with plasma HDL-C, and plasma resistin positively with serum creatinine.
PB  - Croatian Soc Medical Biochemistry & Laboratory Medicine, Zagreb
T2  - Biochemia Medica
T1  - Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease
VL  - 25
IS  - 2
SP  - 242
EP  - 251
DO  - 10.11613/BM.2015.025
ER  - 

@article{
author = "Joksić, Jelena and Sopić, Miron and Spasojević-Kalimanovska, Vesna and Kalimanovska-Oštrić, Dimitra and Anđelković, Kristina and Jelić-Ivanović, Zorana",
year = "2015",
abstract = "Introduction: Previous studies have implicated a strong link between circulating plasma resistin and coronary artery disease (CAD). The aim of this study was to evaluate the differences in peripheral blood mononuclear cells (PBMC) resistin mRNA and its plasma protein concentrations between the patients with CAD of different clinical severity. Material and methods: This study included 33 healthy subjects as the control group (CG) and 77 patients requiring coronary angiography. Of the latter 30 was CAD negative whereas 47 were CAD positive [18 with stable angina pectoris (SAP) and 29 with acute coronary syndrome (ACS)]. Circulating resistin was measured by ELISA; PBMC resistin mRNA was determined by real-time PCR. Results: Resistin protein was significantly higher in the ACS group compared to the CG (P = 0.001) and the CAD negative group (P = 0.018). Resistin mRNA expression did not vary across the study groups, despite the positive correlation seen with plasma resistin (rho = 0.305, P = 0.008). In patients, plasma resistin and PBMC resistin mRNA negatively correlated with HDL-C (rho = -0.404, P  lt  0.001 and rho = -0.257, P = 0.032, respectively). Furthermore, the highest plasma resistin tertile showed the lowest HDL-C (P = 0.006). Plasma resistin was positively associated with serum creatinine (rho = 0.353, P = 0.002). Conclusion: Significant increase of plasma resistin in patients with ACS compared to CG and CAD negative patients was observed. Despite no change in PBMC resistin mRNA in different disease conditions a positive association between resistin mRNA and resistin plasma protein was evident. Both plasma resistin and PBMC resistin mRNA were negatively associated with plasma HDL-C, and plasma resistin positively with serum creatinine.",
publisher = "Croatian Soc Medical Biochemistry & Laboratory Medicine, Zagreb",
journal = "Biochemia Medica",
title = "Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease",
volume = "25",
number = "2",
pages = "242-251",
doi = "10.11613/BM.2015.025"
}

Joksić, J., Sopić, M., Spasojević-Kalimanovska, V., Kalimanovska-Oštrić, D., Anđelković, K.,& Jelić-Ivanović, Z.. (2015). Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease. in Biochemia Medica
Croatian Soc Medical Biochemistry & Laboratory Medicine, Zagreb., 25(2), 242-251.
https://doi.org/10.11613/BM.2015.025

Joksić J, Sopić M, Spasojević-Kalimanovska V, Kalimanovska-Oštrić D, Anđelković K, Jelić-Ivanović Z. Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease. in Biochemia Medica. 2015;25(2):242-251.
doi:10.11613/BM.2015.025 .

Joksić, Jelena, Sopić, Miron, Spasojević-Kalimanovska, Vesna, Kalimanovska-Oštrić, Dimitra, Anđelković, Kristina, Jelić-Ivanović, Zorana, "Circulating resistin protein and mRNA concentrations and clinical severity of coronary artery disease" in Biochemia Medica, 25, no. 2 (2015):242-251,
https://doi.org/10.11613/BM.2015.025 . .