TY  - JOUR
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Savić, Miroslav
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Jančić, Ivan
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4910
AB  - Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords
AB  - Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.
PB  - De Gruyter Poland
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Is alumina suitable for solid phase extraction of catecholamines from brain tissue?
T1  - Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?
VL  - 74
IS  - 2
SP  - 120
EP  - 126
DO  - 10.2478/aiht-2023-74-3706
ER  - 

@article{
author = "Mirković, Duško and Beletić, Anđelo and Savić, Miroslav and Milinković, Neda and Sarić-Matutinović, Marija and Jančić, Ivan",
year = "2023",
abstract = "Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 µm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol. © 2023 Duško Mirković et al., published by Sciendo.  Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2O3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 µm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije. Author keywords aluminijev oksid; aluminium oxide; brain; catecholamines; ekstrakcija čvrste faze; katekolamini; mozak; solid phase extraction; tissue; tkivo   © This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine      1of1   Top of page Cited by 0 documents Inform me when this document is cited in Scopus: Related documents Find more related documents in Scopus based on: Authors Keywords, Promjene razine katekolamina (KAT) u moždanom tkivu značajne su za brojna područja profesionalne toksikologije odnosno ekotoksikologije u kojima se istražuje neurotoksičnost izazvana različitim agensima poput mangana ili cinka, pesticida, industrijskih otapala, plastike, aerozagađenja ili zračenja. Niske koncentracije KAT-a u tkivu zahtijevaju pouzdanu i učinkovitu tehniku ekstrakcije kojom se postiže maksimalni „prinos“ katekolamina i minimalni sadržaj interferirajućih spojeva. Cilj istraživanja bio je procijeniti mogu li se kolone na bazi aluminijeva (III) oksida (Al2 O 3), dizajnirane za izolaciju KAT-a iz plazme, koristiti za ekstrakciju čvrstom fazom (eng. solid-phase extraction – SPE) KAT-a iz moždanoga tkiva. Nakon homogenizacije tkiva Sprague Dawley štakora upotrebom perklorne kiseline, primijenjene su tri tehnike ekstrakcije: SPE, filtracija kroz 0,22 μm membranski filtar, koji je zapravo rutinska tehnika za izolaciju KAT-a iz mozga, i kombinacija tih dviju tehnika. U ekstraktima je relativna kromatografska pokretljivost KAT-a analizirana HPLC metodom s elektrokemijskom detekcijom. Ponašanje norepinefrina i epinefrina tijekom kromatografije bilo je slično, bez obzira na tehniku ekstrakcije, što upućuje na to da aluminijev oksid ima zadovoljavajuća svojstva izolirati ta dva KAT-a iz moždanoga tkiva. Međutim, uočeni su problemi s ekstrakcijom dopamina, koji zahtijevaju dodatne eksperimente kako bi se otkrio njihov uzrok i osmislio protokol optimizacije.",
publisher = "De Gruyter Poland",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?, Je li aluminijev oksid pogodan za postupak ekstrakcije čvrste faze katekolamina iz moždanoga tkiva?",
volume = "74",
number = "2",
pages = "120-126",
doi = "10.2478/aiht-2023-74-3706"
}

Mirković, D., Beletić, A., Savić, M., Milinković, N., Sarić-Matutinović, M.,& Jančić, I.. (2023). Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju
De Gruyter Poland., 74(2), 120-126.
https://doi.org/10.2478/aiht-2023-74-3706

Mirković D, Beletić A, Savić M, Milinković N, Sarić-Matutinović M, Jančić I. Is alumina suitable for solid phase extraction of catecholamines from brain tissue?. in Arhiv za higijenu rada i toksikologiju. 2023;74(2):120-126.
doi:10.2478/aiht-2023-74-3706 .

Mirković, Duško, Beletić, Anđelo, Savić, Miroslav, Milinković, Neda, Sarić-Matutinović, Marija, Jančić, Ivan, "Is alumina suitable for solid phase extraction of catecholamines from brain tissue?" in Arhiv za higijenu rada i toksikologiju, 74, no. 2 (2023):120-126,
https://doi.org/10.2478/aiht-2023-74-3706 . .

TY  - JOUR
AU  - Dudašova Petrovičova, Olina
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
AU  - Dopsaj, Violeta
AU  - Milinković, Neda
AU  - Dopsaj, Milivoj
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5409
AB  - A randomized, double-blind, placebo-controlled study was conducted to investigate the influence of supplementation with a superoxide dismutase (SOD)-rich plant extract on markers of oxidative stress, zonulin levels and the performance of elite athletes. Participants were 30 international-level rowers, divided into an experimental group (n = 15) and a control group (n = 15). The rowers performed a maximal effort incremental test on a rowing ergometer at the beginning and at the end of the study. Markers of oxidative stress (total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), advanced oxidation protein products (AOPPs), malondialdehyde (MDA), sulfhydryl (SH) groups, bilirubin, uric acid, albumin and zonulin) were determined in serum. A lower TOS (p = 0.010) and OSI (p = 0.004), a lower MDA (p = 0.001) and a higher level of SH groups (p = 0.031) were observed in the experimental group after supplementation. Physical performance was evaluated through metabolic efficiency, taking lactate levels and power output on the ergometer into account. After 6 weeks of supplementation, the relative increase in metabolic efficiency at a 4 mmol/L lactate concentration and maximal effort was significantly higher in the experimental group (p = 0.004 and p = 0.015, respectively). These results suggest that supplementation with a SOD-rich extract promotes lower oxidative stress, better antioxidant protection and, consequently, the better work performance of athletes.
PB  - MDPI
T2  - Metabolites
T1  - How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress
VL  - 13
IS  - 12
DO  - 10.3390/metabo13121200
ER  - 

@article{
author = "Dudašova Petrovičova, Olina and Stanković, Ivan and Đorđević, Brižita and Dopsaj, Violeta and Milinković, Neda and Dopsaj, Milivoj",
year = "2023",
abstract = "A randomized, double-blind, placebo-controlled study was conducted to investigate the influence of supplementation with a superoxide dismutase (SOD)-rich plant extract on markers of oxidative stress, zonulin levels and the performance of elite athletes. Participants were 30 international-level rowers, divided into an experimental group (n = 15) and a control group (n = 15). The rowers performed a maximal effort incremental test on a rowing ergometer at the beginning and at the end of the study. Markers of oxidative stress (total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), advanced oxidation protein products (AOPPs), malondialdehyde (MDA), sulfhydryl (SH) groups, bilirubin, uric acid, albumin and zonulin) were determined in serum. A lower TOS (p = 0.010) and OSI (p = 0.004), a lower MDA (p = 0.001) and a higher level of SH groups (p = 0.031) were observed in the experimental group after supplementation. Physical performance was evaluated through metabolic efficiency, taking lactate levels and power output on the ergometer into account. After 6 weeks of supplementation, the relative increase in metabolic efficiency at a 4 mmol/L lactate concentration and maximal effort was significantly higher in the experimental group (p = 0.004 and p = 0.015, respectively). These results suggest that supplementation with a SOD-rich extract promotes lower oxidative stress, better antioxidant protection and, consequently, the better work performance of athletes.",
publisher = "MDPI",
journal = "Metabolites",
title = "How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress",
volume = "13",
number = "12",
doi = "10.3390/metabo13121200"
}

Dudašova Petrovičova, O., Stanković, I., Đorđević, B., Dopsaj, V., Milinković, N.,& Dopsaj, M.. (2023). How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress. in Metabolites
MDPI., 13(12).
https://doi.org/10.3390/metabo13121200

Dudašova Petrovičova O, Stanković I, Đorđević B, Dopsaj V, Milinković N, Dopsaj M. How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress. in Metabolites. 2023;13(12).
doi:10.3390/metabo13121200 .

Dudašova Petrovičova, Olina, Stanković, Ivan, Đorđević, Brižita, Dopsaj, Violeta, Milinković, Neda, Dopsaj, Milivoj, "How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress" in Metabolites, 13, no. 12 (2023),
https://doi.org/10.3390/metabo13121200 . .

TY  - JOUR
AU  - Vasić, Marija
AU  - Topić, Aleksandra
AU  - Marković, Bojan
AU  - Milinković, Neda
AU  - Dinčić, Evica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4397
AB  - Background: Multiple sclerosis (MS) is characterized by
inflammation, demyelination and axonal degeneration.
Oxidative stress (OS) plays a significant role in the pathogenesis of the disease. The aim of the study was to examine the association between OS and smoking on the MS
development.
Methods: The study included 175 patients with relapsing-
remitting multiple sclerosis (RRMS) (76 males, 99 females)
and 254 healthy subjects (81 males and 173 females).
Oxidative stress biomarkers in serum, Total Antioxidant
Status (TAS) and Total Oxidative Status (TOS) were determined spectrophotometrically. Oxidative Stress Index (OSI)
was calculated as the ratio of TOS and TAS. Urinary 8-oxo-
7,8-dihydro-2’-deoxyguanosine were determined by
HPLC-MS/MS and expressed as 8-oxodG/creatinine.
Results: In females with RRMS were higher TOS, OSI and
8-oxodG/creatinine than in females in control group. The
group of males with RRMS had lower level of TAS than the
males in control group. Higher levels of 8-oxodG/creatinine was obtained in active, passive and former smokers
with RRMS than in control group with the same exposition
to tobacco smoke. Independent predictors of MS are passive smoking, increased OSI and increased levels of urinary
8-oxodG/creatinine.
Conclusions: Our results demonstrate that the OS parameters should be included in the assessment of the risk for MS
development. Due to the more sensitivity to oxidative
stress, females may be at higher risk of MS development.
This data indicates the importance of introducing the
antioxidant therapy as a complementary treatment in
patients with RRMS.
AB  - Uvod: Multipla skleroza (MS) se karakteriše upalom, demijelinizacijom i degeneracijom aksona. Oksidativni stres (OS) igra značajnu ulogu u patogenezi bolesti. Cilj studije je bio da se ispita povezanost OS i pušenja na razvoj MS. Metode: Studija je obuhvatila 175 pacijenata sa relapsnoremitentnom multiplom sklerozom (RRMS) (76 muškaraca, 99 žena) i 254 zdrava ispitanika (81 muškarac i 173 žene). Biomarkeri oksidativnog stresa u serumu, ukupni antioksidativni status (TAS) i ukupni oksidativni status (TOS) su određeni spektrofotometrijski. Indeks oksidativnog stresa (OSI) je izračunat kao odnos TOS i TAS. Urinarni 8-okso7,8-dihidro-2'-deoksiguanozin je određen HPLC-MS/MS i izražen kao 8-oksoG/kreatinin. Rezultati: Kod žena sa RRMS bili su viši TOS, OSI i 8okodG/kreatinin nego kod žena u kontrolnoj grupi. Grupa muškaraca sa RRMS imala je niži nivo TAS od muškaraca u kontrolnoj grupi. Veći nivoi 8-okodG/kreatinina su dobijeni kod aktivnih, pasivnih i bivših pušača sa RRMS nego u kontrolnoj grupi sa istom izloženošću duvanskom dimu. Nezavisni prediktori MS su pasivno pušenje, povećan OSI i povećani nivoi 8-okodG/kreatinina u urinu. Zaključak: Naši rezultati pokazuju da parametre OS treba uključiti u procenu rizika za razvoj MS. Zbog veće osetljivosti na oksidativni stres, žene mogu biti izložene većem riziku od razvoja MS. Ovi podaci ukazuju na značaj uvođenja antioksidativne terapije kao komplementarnog lečenja kod pacijenata sa RRMS.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Oxidative stress-related risk of the multiple sclerosis development
T1  - Rizik razvoja multiple skleroze povezan sa oksidativnim stresom
VL  - 42
IS  - 1
SP  - 1
EP  - 8
DO  - 10.5937/jomb0-37546
ER  - 

@article{
author = "Vasić, Marija and Topić, Aleksandra and Marković, Bojan and Milinković, Neda and Dinčić, Evica",
year = "2023",
abstract = "Background: Multiple sclerosis (MS) is characterized by
inflammation, demyelination and axonal degeneration.
Oxidative stress (OS) plays a significant role in the pathogenesis of the disease. The aim of the study was to examine the association between OS and smoking on the MS
development.
Methods: The study included 175 patients with relapsing-
remitting multiple sclerosis (RRMS) (76 males, 99 females)
and 254 healthy subjects (81 males and 173 females).
Oxidative stress biomarkers in serum, Total Antioxidant
Status (TAS) and Total Oxidative Status (TOS) were determined spectrophotometrically. Oxidative Stress Index (OSI)
was calculated as the ratio of TOS and TAS. Urinary 8-oxo-
7,8-dihydro-2’-deoxyguanosine were determined by
HPLC-MS/MS and expressed as 8-oxodG/creatinine.
Results: In females with RRMS were higher TOS, OSI and
8-oxodG/creatinine than in females in control group. The
group of males with RRMS had lower level of TAS than the
males in control group. Higher levels of 8-oxodG/creatinine was obtained in active, passive and former smokers
with RRMS than in control group with the same exposition
to tobacco smoke. Independent predictors of MS are passive smoking, increased OSI and increased levels of urinary
8-oxodG/creatinine.
Conclusions: Our results demonstrate that the OS parameters should be included in the assessment of the risk for MS
development. Due to the more sensitivity to oxidative
stress, females may be at higher risk of MS development.
This data indicates the importance of introducing the
antioxidant therapy as a complementary treatment in
patients with RRMS., Uvod: Multipla skleroza (MS) se karakteriše upalom, demijelinizacijom i degeneracijom aksona. Oksidativni stres (OS) igra značajnu ulogu u patogenezi bolesti. Cilj studije je bio da se ispita povezanost OS i pušenja na razvoj MS. Metode: Studija je obuhvatila 175 pacijenata sa relapsnoremitentnom multiplom sklerozom (RRMS) (76 muškaraca, 99 žena) i 254 zdrava ispitanika (81 muškarac i 173 žene). Biomarkeri oksidativnog stresa u serumu, ukupni antioksidativni status (TAS) i ukupni oksidativni status (TOS) su određeni spektrofotometrijski. Indeks oksidativnog stresa (OSI) je izračunat kao odnos TOS i TAS. Urinarni 8-okso7,8-dihidro-2'-deoksiguanozin je određen HPLC-MS/MS i izražen kao 8-oksoG/kreatinin. Rezultati: Kod žena sa RRMS bili su viši TOS, OSI i 8okodG/kreatinin nego kod žena u kontrolnoj grupi. Grupa muškaraca sa RRMS imala je niži nivo TAS od muškaraca u kontrolnoj grupi. Veći nivoi 8-okodG/kreatinina su dobijeni kod aktivnih, pasivnih i bivših pušača sa RRMS nego u kontrolnoj grupi sa istom izloženošću duvanskom dimu. Nezavisni prediktori MS su pasivno pušenje, povećan OSI i povećani nivoi 8-okodG/kreatinina u urinu. Zaključak: Naši rezultati pokazuju da parametre OS treba uključiti u procenu rizika za razvoj MS. Zbog veće osetljivosti na oksidativni stres, žene mogu biti izložene većem riziku od razvoja MS. Ovi podaci ukazuju na značaj uvođenja antioksidativne terapije kao komplementarnog lečenja kod pacijenata sa RRMS.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Oxidative stress-related risk of the multiple sclerosis development, Rizik razvoja multiple skleroze povezan sa oksidativnim stresom",
volume = "42",
number = "1",
pages = "1-8",
doi = "10.5937/jomb0-37546"
}

Vasić, M., Topić, A., Marković, B., Milinković, N.,& Dinčić, E.. (2023). Oxidative stress-related risk of the multiple sclerosis development. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 42(1), 1-8.
https://doi.org/10.5937/jomb0-37546

Vasić M, Topić A, Marković B, Milinković N, Dinčić E. Oxidative stress-related risk of the multiple sclerosis development. in Journal of Medical Biochemistry. 2023;42(1):1-8.
doi:10.5937/jomb0-37546 .

Vasić, Marija, Topić, Aleksandra, Marković, Bojan, Milinković, Neda, Dinčić, Evica, "Oxidative stress-related risk of the multiple sclerosis development" in Journal of Medical Biochemistry, 42, no. 1 (2023):1-8,
https://doi.org/10.5937/jomb0-37546 . .

TY  - JOUR
AU  - Džoljić, Eleonora
AU  - Sarić-Matutinović, Marija
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Milinković, Neda
AU  - Kostić, Vladimir
AU  - Ignjatović, Svetlana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5179
AB  - Background: Vitamin D is a steroid hormone, known to be involved in the pathogenesis of various neurodegenerative disorders, including Parkinson's disease (PD). We aimed to clarify the relationship between hypovitaminosis D and the predisposition for PD and its clinical presentation. An additional aim was to examine the specific gene polymorphisms associated with vitamin D level. Material and methods: Total level of 25(OH)-vitamin D (25(OH)D) was measured in the serum of parkinsonian patients (n = 113) and controls (n = 82) using a commercial immunoassay. Genetic analyses were performed using Taqman assays on Real Time PCR amplification system. Results: Higher frequency of vitamin D deficiency (<50 nmol/L) was observed in PD patients, compared to controls (40.7% and 23.2%, respectively, P = 0.010). It was also a positive predictive marker of PD (OR, 2.27; 95% CI, 1.206–4.298; P < 0.011). Significantly higher UPDRS (35.85 ± 1.35 and 32.09 ± 0.99, respectively, P = 0.023) and HY scores (2(1.5–2.5) and 1.5(1.0–2.0), respectively, P = 0.005) were present in patients with 25(OH)D level < 50 nmol/L compared to patients with 25(OH)D level ≥ 50 nmol/L. Despite some trends observed, differences in allelic and genotypic distribution between controls and patients, as well as between subgroups, did not reach the level of significance (P > 0.05). Conclusions: Findings of this study confirm the hypothesis of a significant relationship between hypovitaminosis D and PD. We demonstrated higher prevalence of vitamin D deficiency in PD patients, as well as its predictive potential for the onset and progression of PD.
PB  - Elsevier Ltd
T2  - Neuroscience
T1  - Vitamin D Serum Levels and Vitamin D Receptor Genotype in Patients with Parkinson's Disease
VL  - 533
SP  - 53
EP  - 62
DO  - 10.1016/j.neuroscience.2023.10.004
ER  - 

@article{
author = "Džoljić, Eleonora and Sarić-Matutinović, Marija and Stojković, Oliver and Veličković, Jelena and Milinković, Neda and Kostić, Vladimir and Ignjatović, Svetlana",
year = "2023",
abstract = "Background: Vitamin D is a steroid hormone, known to be involved in the pathogenesis of various neurodegenerative disorders, including Parkinson's disease (PD). We aimed to clarify the relationship between hypovitaminosis D and the predisposition for PD and its clinical presentation. An additional aim was to examine the specific gene polymorphisms associated with vitamin D level. Material and methods: Total level of 25(OH)-vitamin D (25(OH)D) was measured in the serum of parkinsonian patients (n = 113) and controls (n = 82) using a commercial immunoassay. Genetic analyses were performed using Taqman assays on Real Time PCR amplification system. Results: Higher frequency of vitamin D deficiency (<50 nmol/L) was observed in PD patients, compared to controls (40.7% and 23.2%, respectively, P = 0.010). It was also a positive predictive marker of PD (OR, 2.27; 95% CI, 1.206–4.298; P < 0.011). Significantly higher UPDRS (35.85 ± 1.35 and 32.09 ± 0.99, respectively, P = 0.023) and HY scores (2(1.5–2.5) and 1.5(1.0–2.0), respectively, P = 0.005) were present in patients with 25(OH)D level < 50 nmol/L compared to patients with 25(OH)D level ≥ 50 nmol/L. Despite some trends observed, differences in allelic and genotypic distribution between controls and patients, as well as between subgroups, did not reach the level of significance (P > 0.05). Conclusions: Findings of this study confirm the hypothesis of a significant relationship between hypovitaminosis D and PD. We demonstrated higher prevalence of vitamin D deficiency in PD patients, as well as its predictive potential for the onset and progression of PD.",
publisher = "Elsevier Ltd",
journal = "Neuroscience",
title = "Vitamin D Serum Levels and Vitamin D Receptor Genotype in Patients with Parkinson's Disease",
volume = "533",
pages = "53-62",
doi = "10.1016/j.neuroscience.2023.10.004"
}

Džoljić, E., Sarić-Matutinović, M., Stojković, O., Veličković, J., Milinković, N., Kostić, V.,& Ignjatović, S.. (2023). Vitamin D Serum Levels and Vitamin D Receptor Genotype in Patients with Parkinson's Disease. in Neuroscience
Elsevier Ltd., 533, 53-62.
https://doi.org/10.1016/j.neuroscience.2023.10.004

Džoljić E, Sarić-Matutinović M, Stojković O, Veličković J, Milinković N, Kostić V, Ignjatović S. Vitamin D Serum Levels and Vitamin D Receptor Genotype in Patients with Parkinson's Disease. in Neuroscience. 2023;533:53-62.
doi:10.1016/j.neuroscience.2023.10.004 .

Džoljić, Eleonora, Sarić-Matutinović, Marija, Stojković, Oliver, Veličković, Jelena, Milinković, Neda, Kostić, Vladimir, Ignjatović, Svetlana, "Vitamin D Serum Levels and Vitamin D Receptor Genotype in Patients with Parkinson's Disease" in Neuroscience, 533 (2023):53-62,
https://doi.org/10.1016/j.neuroscience.2023.10.004 . .

TY  - JOUR
AU  - Çubukçu, Hikmet Can
AU  - Vanstapel, Florent
AU  - Thelen, Marc
AU  - Van Schrojenstein Lantman, Marith
AU  - Bernabeu-Andreu, Francisco A
AU  - Meško Brguljan, Pika
AU  - Milinković, Neda
AU  - Linko, Solveig
AU  - Panteghini, Mauro
AU  - Boursier, Guilaine
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5345
AB  - According to ISO 15189:2022, analytical performance specifications (APS) should relate to intended clinical use and impact on patient care. Therefore, we aimed to develop a web application for laboratory professionals to calculate APS based on a simulation of the impact of measurement uncertainty (MU) on the outcome using the chosen decision limits, agreement thresholds, and data of the population of interest. We developed the "APS Calculator"allowing users to upload and select data of concern, specify decision limits and agreement thresholds, and conduct simulations to determine APS for MU. The simulation involved categorizing original measurand concentrations, generating measured (simulated) results by introducing different degrees of MU, and recategorizing measured concentrations based on clinical decision limits and acceptable clinical misclassification rates. The agreements between original and simulated result categories were assessed, and values that met or exceeded user-specified agreement thresholds that set goals for the between-category agreement were considered acceptable. The application generates contour plots of agreement rates and corresponding MU values. We tested the application using National Health and Nutrition Examination Survey data, with decision limits from relevant guidelines. We determined APS for MU of six measurands (blood total hemoglobin, plasma fasting glucose, serum total and high-density lipoprotein cholesterol, triglycerides, and total folate) to demonstrate the potential of the application to generate APS. The developed data-driven web application offers a flexible tool for laboratory professionals to calculate APS for MU using their chosen decision limits and agreement thresholds, and the data of the population of interest.
PB  - Walter de Gruyter GmbH
T2  - Clinical Chemistry and Laboratory Medicine
T1  - APS calculator: A data-driven tool for setting outcome-based analytical performance specifications for measurement uncertainty using specific clinical requirements and population data
DO  - 10.1515/cclm-2023-0740
ER  - 

@article{
author = "Çubukçu, Hikmet Can and Vanstapel, Florent and Thelen, Marc and Van Schrojenstein Lantman, Marith and Bernabeu-Andreu, Francisco A and Meško Brguljan, Pika and Milinković, Neda and Linko, Solveig and Panteghini, Mauro and Boursier, Guilaine",
year = "2023",
abstract = "According to ISO 15189:2022, analytical performance specifications (APS) should relate to intended clinical use and impact on patient care. Therefore, we aimed to develop a web application for laboratory professionals to calculate APS based on a simulation of the impact of measurement uncertainty (MU) on the outcome using the chosen decision limits, agreement thresholds, and data of the population of interest. We developed the "APS Calculator"allowing users to upload and select data of concern, specify decision limits and agreement thresholds, and conduct simulations to determine APS for MU. The simulation involved categorizing original measurand concentrations, generating measured (simulated) results by introducing different degrees of MU, and recategorizing measured concentrations based on clinical decision limits and acceptable clinical misclassification rates. The agreements between original and simulated result categories were assessed, and values that met or exceeded user-specified agreement thresholds that set goals for the between-category agreement were considered acceptable. The application generates contour plots of agreement rates and corresponding MU values. We tested the application using National Health and Nutrition Examination Survey data, with decision limits from relevant guidelines. We determined APS for MU of six measurands (blood total hemoglobin, plasma fasting glucose, serum total and high-density lipoprotein cholesterol, triglycerides, and total folate) to demonstrate the potential of the application to generate APS. The developed data-driven web application offers a flexible tool for laboratory professionals to calculate APS for MU using their chosen decision limits and agreement thresholds, and the data of the population of interest.",
publisher = "Walter de Gruyter GmbH",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "APS calculator: A data-driven tool for setting outcome-based analytical performance specifications for measurement uncertainty using specific clinical requirements and population data",
doi = "10.1515/cclm-2023-0740"
}

Çubukçu, H. C., Vanstapel, F., Thelen, M., Van Schrojenstein Lantman, M., Bernabeu-Andreu, F. A., Meško Brguljan, P., Milinković, N., Linko, S., Panteghini, M.,& Boursier, G.. (2023). APS calculator: A data-driven tool for setting outcome-based analytical performance specifications for measurement uncertainty using specific clinical requirements and population data. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter GmbH..
https://doi.org/10.1515/cclm-2023-0740

Çubukçu HC, Vanstapel F, Thelen M, Van Schrojenstein Lantman M, Bernabeu-Andreu FA, Meško Brguljan P, Milinković N, Linko S, Panteghini M, Boursier G. APS calculator: A data-driven tool for setting outcome-based analytical performance specifications for measurement uncertainty using specific clinical requirements and population data. in Clinical Chemistry and Laboratory Medicine. 2023;.
doi:10.1515/cclm-2023-0740 .

Çubukçu, Hikmet Can, Vanstapel, Florent, Thelen, Marc, Van Schrojenstein Lantman, Marith, Bernabeu-Andreu, Francisco A, Meško Brguljan, Pika, Milinković, Neda, Linko, Solveig, Panteghini, Mauro, Boursier, Guilaine, "APS calculator: A data-driven tool for setting outcome-based analytical performance specifications for measurement uncertainty using specific clinical requirements and population data" in Clinical Chemistry and Laboratory Medicine (2023),
https://doi.org/10.1515/cclm-2023-0740 . .

TY  - JOUR
AU  - Okuka, Nina
AU  - Schuh, Verena
AU  - Krammer, Ulrike
AU  - Polovina, Snežana
AU  - Šumarac-Dumanović, Mirjana
AU  - Milinković, Neda
AU  - Veličković, Ksenija
AU  - Đorđević, Brižita
AU  - Haslberger, Alexander
AU  - Ivanović, Nevena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5054
AB  - Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.
PB  - MDPI
T2  - Life
T1  - Epigenetic Aspects of a New Probiotic Concept—A Pilot Study
VL  - 13
IS  - 9
DO  - 10.3390/life13091912
ER  - 

@article{
author = "Okuka, Nina and Schuh, Verena and Krammer, Ulrike and Polovina, Snežana and Šumarac-Dumanović, Mirjana and Milinković, Neda and Veličković, Ksenija and Đorđević, Brižita and Haslberger, Alexander and Ivanović, Nevena",
year = "2023",
abstract = "Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.",
publisher = "MDPI",
journal = "Life",
title = "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study",
volume = "13",
number = "9",
doi = "10.3390/life13091912"
}

Okuka, N., Schuh, V., Krammer, U., Polovina, S., Šumarac-Dumanović, M., Milinković, N., Veličković, K., Đorđević, B., Haslberger, A.,& Ivanović, N.. (2023). Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life
MDPI., 13(9).
https://doi.org/10.3390/life13091912

Okuka N, Schuh V, Krammer U, Polovina S, Šumarac-Dumanović M, Milinković N, Veličković K, Đorđević B, Haslberger A, Ivanović N. Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life. 2023;13(9).
doi:10.3390/life13091912 .

Okuka, Nina, Schuh, Verena, Krammer, Ulrike, Polovina, Snežana, Šumarac-Dumanović, Mirjana, Milinković, Neda, Veličković, Ksenija, Đorđević, Brižita, Haslberger, Alexander, Ivanović, Nevena, "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study" in Life, 13, no. 9 (2023),
https://doi.org/10.3390/life13091912 . .

TY  - JOUR
AU  - Jegorović, Boris
AU  - Nikolić, Aleksandra
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Šipetić Grujičić, Sandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4993
AB  - Background: The unpredictable course of Coronavirus
Disease 19 (COVID-19) is making good severity assessment tools crucial. This study aimed to assess the usefulness of serum amyloid A (SAA) and other acute-phase
reactants (APRs) in ambulatory care COVID-19 patients
and identified relationships between these markers and disease outcomes.
Methods: From August to November 2020, patients seen
in the outpatient department of the Clinic for Infectious and
Tropical Diseases (Belgrade, Serbia) with confirmed
COVID-19 were included. Patients were classified into
mild, moderate, and severe disease groups based on World
Health Organization criteria. SAA, C-reactive protein
(CRP), interleukin-6 (IL-6), procalcitonin (PCT), ferritin, fibrinogen, D-dimer, albumin, and transferrin were measured.
The median values of all APRs were compared between
COVID-19 severity groups, hospitalized and non-hospitalized patients, and survivors and non-survivors. The
Receiver operator characteristic (ROC) curve analysis was
used for the classification characteristics assessment of
individual APRs for the severity of illness, hospitalization,
and survival.
Results: Higher levels of SAA, CRP, IL-6, PCT, and lower
levels of transferrin and albumin were observed in severe
cases, hospitalized patients, and non-survivors. Based on
ROC curve analysis AUC for SAA has fair classification performance for disease severity (0.794) and death (0.732)
and good performance for hospitalization (0.853).
Conclusion: SAA is a valuable marker in everyday practice
for assessing COVID-19 severity and prognosis in ambulatory patients.
AB  - Uvod: Tok koronavirusne bolesti 2019 (COVID-19) je nepredvidiv, zbog čega su neophodni dobri alati za procenu težine ove infekcije. Svrha ovog istraživanja je procena značaja određivanja serumskog amiloida A (SAA) i drugih reaktanata akutne faze (RAF) kod ambulantnih pacijenata sa COVID-19, kao i identifikacija veze između ovih markera i ishoda bolesti. Metode: Od avgusta do novembra 2020. godine, u studju su uključeni pacijenti koji su pregledani u Prijemnoj ambulanti Klinike za infektivne i tropske bolesti (Beograd, Srbija), sa potvrđenim COVID-19. Pacijenti su bili klasifikovani u grupe blage, umerene i teške bolesti na osnovu kriterijuma Svetske zdravstvene organizacije. Određivane su vrednosti SAA, C-reaktivnog proteina (CRP), interleukina-6 (IL-6), prokalcitonina (PCT), feritina, fibrinogena, D-dimera, albumina i transferina. Medijane vrednosti RAF su poređene između grupa težine bolesti, bolesnika koji su bili hospitalizovani i onih koji nisu, kao i preživelih i preminulih. Korišćena je analiza Receiver operator characteristic (ROC) krive i Area-under-curve (AUC) za analizu klasifikacionih karakteristika pojedinačnih RAF za težinu bolesti, hospitalizaciju i preživljavanje. Rezultati: Viši nivoi SAA, CRP, IL-6, PCT i niži nivoi transferrina i albumina su uočeni kod osoba sa teškim oblikom bolesti, hospitalizovanih pacijenata i preminulih. Na osnovu analize ROC krive, AUC za SAA je pokazao zadovoljavajuće klasifikacione performanse za težinu bolesti (0,794) i smrtni ishod (0,732) i dobre performanse za hospitalizaciju (0,853). Zaključak: SAA je vredan marker u svakodnevnoj praksi za procenu težine i prognoze COVID-19 kod ambulantnih pacijenata.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients
T1  - Značaj određivanja serumskog amiloida A i drugih reaktanata akutne faze kod ambulantnih pacijenata sa COVID-19
VL  - 42
IS  - 3
SP  - 492
EP  - 504
DO  - 10.5937/jomb0-42799
ER  - 

@article{
author = "Jegorović, Boris and Nikolić, Aleksandra and Milinković, Neda and Ignjatović, Svetlana and Šipetić Grujičić, Sandra",
year = "2023",
abstract = "Background: The unpredictable course of Coronavirus
Disease 19 (COVID-19) is making good severity assessment tools crucial. This study aimed to assess the usefulness of serum amyloid A (SAA) and other acute-phase
reactants (APRs) in ambulatory care COVID-19 patients
and identified relationships between these markers and disease outcomes.
Methods: From August to November 2020, patients seen
in the outpatient department of the Clinic for Infectious and
Tropical Diseases (Belgrade, Serbia) with confirmed
COVID-19 were included. Patients were classified into
mild, moderate, and severe disease groups based on World
Health Organization criteria. SAA, C-reactive protein
(CRP), interleukin-6 (IL-6), procalcitonin (PCT), ferritin, fibrinogen, D-dimer, albumin, and transferrin were measured.
The median values of all APRs were compared between
COVID-19 severity groups, hospitalized and non-hospitalized patients, and survivors and non-survivors. The
Receiver operator characteristic (ROC) curve analysis was
used for the classification characteristics assessment of
individual APRs for the severity of illness, hospitalization,
and survival.
Results: Higher levels of SAA, CRP, IL-6, PCT, and lower
levels of transferrin and albumin were observed in severe
cases, hospitalized patients, and non-survivors. Based on
ROC curve analysis AUC for SAA has fair classification performance for disease severity (0.794) and death (0.732)
and good performance for hospitalization (0.853).
Conclusion: SAA is a valuable marker in everyday practice
for assessing COVID-19 severity and prognosis in ambulatory patients., Uvod: Tok koronavirusne bolesti 2019 (COVID-19) je nepredvidiv, zbog čega su neophodni dobri alati za procenu težine ove infekcije. Svrha ovog istraživanja je procena značaja određivanja serumskog amiloida A (SAA) i drugih reaktanata akutne faze (RAF) kod ambulantnih pacijenata sa COVID-19, kao i identifikacija veze između ovih markera i ishoda bolesti. Metode: Od avgusta do novembra 2020. godine, u studju su uključeni pacijenti koji su pregledani u Prijemnoj ambulanti Klinike za infektivne i tropske bolesti (Beograd, Srbija), sa potvrđenim COVID-19. Pacijenti su bili klasifikovani u grupe blage, umerene i teške bolesti na osnovu kriterijuma Svetske zdravstvene organizacije. Određivane su vrednosti SAA, C-reaktivnog proteina (CRP), interleukina-6 (IL-6), prokalcitonina (PCT), feritina, fibrinogena, D-dimera, albumina i transferina. Medijane vrednosti RAF su poređene između grupa težine bolesti, bolesnika koji su bili hospitalizovani i onih koji nisu, kao i preživelih i preminulih. Korišćena je analiza Receiver operator characteristic (ROC) krive i Area-under-curve (AUC) za analizu klasifikacionih karakteristika pojedinačnih RAF za težinu bolesti, hospitalizaciju i preživljavanje. Rezultati: Viši nivoi SAA, CRP, IL-6, PCT i niži nivoi transferrina i albumina su uočeni kod osoba sa teškim oblikom bolesti, hospitalizovanih pacijenata i preminulih. Na osnovu analize ROC krive, AUC za SAA je pokazao zadovoljavajuće klasifikacione performanse za težinu bolesti (0,794) i smrtni ishod (0,732) i dobre performanse za hospitalizaciju (0,853). Zaključak: SAA je vredan marker u svakodnevnoj praksi za procenu težine i prognoze COVID-19 kod ambulantnih pacijenata.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients, Značaj određivanja serumskog amiloida A i drugih reaktanata akutne faze kod ambulantnih pacijenata sa COVID-19",
volume = "42",
number = "3",
pages = "492-504",
doi = "10.5937/jomb0-42799"
}

Jegorović, B., Nikolić, A., Milinković, N., Ignjatović, S.,& Šipetić Grujičić, S.. (2023). The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 42(3), 492-504.
https://doi.org/10.5937/jomb0-42799

Jegorović B, Nikolić A, Milinković N, Ignjatović S, Šipetić Grujičić S. The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients. in Journal of Medical Biochemistry. 2023;42(3):492-504.
doi:10.5937/jomb0-42799 .

Jegorović, Boris, Nikolić, Aleksandra, Milinković, Neda, Ignjatović, Svetlana, Šipetić Grujičić, Sandra, "The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients" in Journal of Medical Biochemistry, 42, no. 3 (2023):492-504,
https://doi.org/10.5937/jomb0-42799 . .

TY  - CONF
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Tadić, Ivana
AU  - Dmitrašinović, Gordana
AU  - Ignjatović, Svetlana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5591
AB  - Uvod i cilj. Literaturni podaci ukazuju da serološka imunoodređivanja mogu biti adekvatna
dopuna molekularnoj dijagnostici SARS-CoV-2 infekcije. Cilj ovog ispitivanja je da se proceni
slaganje SARS-CoV-2 seroloških testova pet različitih proizvođača i ispita imuni odgovor na
različite vrste vakcina.
Metode. Korišćene su metode: Sars-CoV-2 IgG (Beckman Coulter) (I), SARS-CoV-2 ukupna
antitela (BioRad) (II), SARS-CoV-2 IgG II Quant (Abbott) (III), SARS-CoV-2 IgG (Abbott) (IV) i
Elecsys Anti-SARS-CoV-2 (Roche) (V). Paralelno je ispitivano metodom I i II 44 uzorka, I i III 84
uzorka, I i IV 53 uzorka, I i V 80 uzoraka. Dodatno je za procenu imunog odgovora na infekciju i
različite vrste vakcina analizirano 153 uzorka. Ispitanici su proveravali prisustvo antitela zbog
sumnje na asimptomatsku SARS-CoV-2 infekciju ili zbog prisustva simptoma.
Rezultati. II i III metodom je dobijen značajno veći broj reaktivnih rezultata u odnosu na I
metod (65,9% i 91,7% vs 52,3%). I i IV metode su pokazale slaganje u reaktivnosti (60,4% vs
69,8%), dok I i V metodom nije dobijeno slaganje u reaktivnosti rezultata (58,8% vs 93,8%).
Najintenzivniji imuni odgovor postignut je nakon vakcinacije Phizer vakcinom. Imuni odgovor
nakon Phizer i Sinopharm vakcine je pokazao značajnu korelaciju sa vremenom proteklim od
imunizacije (P=0,032 i P=0,012). Žene su imale značajno viši titar antitela nakon vakcinacije
u odnosu na muškarce (P=0,006). Nije dobijena značajna razlika u odnosu na tip vakcine i
godina starosti ispitanika (P=0,197).
Zaključak
Iako serološka imunoodređivanja pokazuju značajne analitičke i kliničke karakteristike za
detekciju SARS-CoV-2 i dalje postoji problem kompletne evaluacije testova i neizvesnosti u
pogledu tačnosti rezultata i njihove uporedivosti.
PB  - Udruženje farmaceuta Beograd
C3  - Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
T1  - Analitičke i kliničke karakteristike seroloških imonoodređivanja anti-SARS-CoV-2 antitela
SP  - 12
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5591
ER  - 

@conference{
author = "Milinković, Neda and Sarić-Matutinović, Marija and Tadić, Ivana and Dmitrašinović, Gordana and Ignjatović, Svetlana",
year = "2022",
abstract = "Uvod i cilj. Literaturni podaci ukazuju da serološka imunoodređivanja mogu biti adekvatna
dopuna molekularnoj dijagnostici SARS-CoV-2 infekcije. Cilj ovog ispitivanja je da se proceni
slaganje SARS-CoV-2 seroloških testova pet različitih proizvođača i ispita imuni odgovor na
različite vrste vakcina.
Metode. Korišćene su metode: Sars-CoV-2 IgG (Beckman Coulter) (I), SARS-CoV-2 ukupna
antitela (BioRad) (II), SARS-CoV-2 IgG II Quant (Abbott) (III), SARS-CoV-2 IgG (Abbott) (IV) i
Elecsys Anti-SARS-CoV-2 (Roche) (V). Paralelno je ispitivano metodom I i II 44 uzorka, I i III 84
uzorka, I i IV 53 uzorka, I i V 80 uzoraka. Dodatno je za procenu imunog odgovora na infekciju i
različite vrste vakcina analizirano 153 uzorka. Ispitanici su proveravali prisustvo antitela zbog
sumnje na asimptomatsku SARS-CoV-2 infekciju ili zbog prisustva simptoma.
Rezultati. II i III metodom je dobijen značajno veći broj reaktivnih rezultata u odnosu na I
metod (65,9% i 91,7% vs 52,3%). I i IV metode su pokazale slaganje u reaktivnosti (60,4% vs
69,8%), dok I i V metodom nije dobijeno slaganje u reaktivnosti rezultata (58,8% vs 93,8%).
Najintenzivniji imuni odgovor postignut je nakon vakcinacije Phizer vakcinom. Imuni odgovor
nakon Phizer i Sinopharm vakcine je pokazao značajnu korelaciju sa vremenom proteklim od
imunizacije (P=0,032 i P=0,012). Žene su imale značajno viši titar antitela nakon vakcinacije
u odnosu na muškarce (P=0,006). Nije dobijena značajna razlika u odnosu na tip vakcine i
godina starosti ispitanika (P=0,197).
Zaključak
Iako serološka imunoodređivanja pokazuju značajne analitičke i kliničke karakteristike za
detekciju SARS-CoV-2 i dalje postoji problem kompletne evaluacije testova i neizvesnosti u
pogledu tačnosti rezultata i njihove uporedivosti.",
publisher = "Udruženje farmaceuta Beograd",
journal = "Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd",
title = "Analitičke i kliničke karakteristike seroloških imonoodređivanja anti-SARS-CoV-2 antitela",
pages = "12-12",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5591"
}

Milinković, N., Sarić-Matutinović, M., Tadić, I., Dmitrašinović, G.,& Ignjatović, S.. (2022). Analitičke i kliničke karakteristike seroloških imonoodređivanja anti-SARS-CoV-2 antitela. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
Udruženje farmaceuta Beograd., 12-12.
https://hdl.handle.net/21.15107/rcub_farfar_5591

Milinković N, Sarić-Matutinović M, Tadić I, Dmitrašinović G, Ignjatović S. Analitičke i kliničke karakteristike seroloških imonoodređivanja anti-SARS-CoV-2 antitela. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd. 2022;:12-12.
https://hdl.handle.net/21.15107/rcub_farfar_5591 .

Milinković, Neda, Sarić-Matutinović, Marija, Tadić, Ivana, Dmitrašinović, Gordana, Ignjatović, Svetlana, "Analitičke i kliničke karakteristike seroloških imonoodređivanja anti-SARS-CoV-2 antitela" in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd (2022):12-12,
https://hdl.handle.net/21.15107/rcub_farfar_5591 .

TY  - CONF
AU  - Sarić-Matutinović, Marija
AU  - Milinković, Neda
AU  - Dmitrašinović, Gordana
AU  - Ignjatović, Svetlana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5590
AB  - Uvod i cilj. Pandemija bolesti izazvane teškim akutnim respiratornim sindromom korona-
virus 2 (SARS-CoV-2) dovela je do ekspanzije seroloških testova za određivanje antitela i an-
tigena virusa. Access SARS-CoV-2 IgG je serološki test namenjen je za detekciju IgG antitela
virusa, i može se koristiti kao dodatno sredstvo prilikom detekcije patogena, praćenja stanja
pacijenata, procene imuniteta nakon infekcije i vakcinacije. Cilj istraživanja je bio da se odre-
de dijagnostičke karakteristike i klinički značaj Access SARS-CoV-2 IgG testa.
Metode. Istraživanjem je obuhvaćeno 94 ispitanika sa potvrđenom prethodnom SARS-
CoV-2 infekcijom (PCR metodom) i 104 kontrolna ispitanika. IgG antitela su određena u se-
rumu ispitanika primenom Access Sars-CoV-2 IgG testa, proizvođača Beckman Coulter, na
imunohemijskom Access 2 imunoanalizatoru iste firme.
Rezultati. Titar IgG antitela je bio značajno veći kod ispitanika koji su preležali infekciju
(3,95 (1,36 - 11,44 S/CO) u odnosu na kontrolne ispitanike (0,03 (0,02 – 0,16 S/CO) (p <
0,001). ROC analizom je utvrđena statistički značajna AUC (0,946, p < 0,001), a osetljivost i
specifičnost za potvrdu infekcije su redom bile 81% i 95%, za graničnu vrednost 0,955. Pozitiv-
na i negativna prediktivna vrednost su bile 94% i 85%, redom. Dodatno je utvrđeno da je titar
IgG antitela bio najveći u grupi ispitanika sa teškim oblikom infekcije, u poređenju sa ispita-
nicima koji su imali lakši oblik i ispitanicima koji su imali blagu ili asimptomatsku infekciju (p
= 0,048).
Zaključak. Naši rezultati ukazuju na to da analizirani Access SARS-CoV-2 IgG serološki
test ima zadovoljavajuće dijagnostičke karakteristike i može biti klinički značajan za procenu
stanja imuniteta nakon preležane infekcije.
PB  - Udruženje farmaceuta Beograd
C3  - Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
T1  - Dijagnostičke karakteristike serološkog access SARS-CoV-2 IgG imunohemijskog testa
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5590
ER  - 

@conference{
author = "Sarić-Matutinović, Marija and Milinković, Neda and Dmitrašinović, Gordana and Ignjatović, Svetlana",
year = "2022",
abstract = "Uvod i cilj. Pandemija bolesti izazvane teškim akutnim respiratornim sindromom korona-
virus 2 (SARS-CoV-2) dovela je do ekspanzije seroloških testova za određivanje antitela i an-
tigena virusa. Access SARS-CoV-2 IgG je serološki test namenjen je za detekciju IgG antitela
virusa, i može se koristiti kao dodatno sredstvo prilikom detekcije patogena, praćenja stanja
pacijenata, procene imuniteta nakon infekcije i vakcinacije. Cilj istraživanja je bio da se odre-
de dijagnostičke karakteristike i klinički značaj Access SARS-CoV-2 IgG testa.
Metode. Istraživanjem je obuhvaćeno 94 ispitanika sa potvrđenom prethodnom SARS-
CoV-2 infekcijom (PCR metodom) i 104 kontrolna ispitanika. IgG antitela su određena u se-
rumu ispitanika primenom Access Sars-CoV-2 IgG testa, proizvođača Beckman Coulter, na
imunohemijskom Access 2 imunoanalizatoru iste firme.
Rezultati. Titar IgG antitela je bio značajno veći kod ispitanika koji su preležali infekciju
(3,95 (1,36 - 11,44 S/CO) u odnosu na kontrolne ispitanike (0,03 (0,02 – 0,16 S/CO) (p <
0,001). ROC analizom je utvrđena statistički značajna AUC (0,946, p < 0,001), a osetljivost i
specifičnost za potvrdu infekcije su redom bile 81% i 95%, za graničnu vrednost 0,955. Pozitiv-
na i negativna prediktivna vrednost su bile 94% i 85%, redom. Dodatno je utvrđeno da je titar
IgG antitela bio najveći u grupi ispitanika sa teškim oblikom infekcije, u poređenju sa ispita-
nicima koji su imali lakši oblik i ispitanicima koji su imali blagu ili asimptomatsku infekciju (p
= 0,048).
Zaključak. Naši rezultati ukazuju na to da analizirani Access SARS-CoV-2 IgG serološki
test ima zadovoljavajuće dijagnostičke karakteristike i može biti klinički značajan za procenu
stanja imuniteta nakon preležane infekcije.",
publisher = "Udruženje farmaceuta Beograd",
journal = "Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd",
title = "Dijagnostičke karakteristike serološkog access SARS-CoV-2 IgG imunohemijskog testa",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5590"
}

Sarić-Matutinović, M., Milinković, N., Dmitrašinović, G.,& Ignjatović, S.. (2022). Dijagnostičke karakteristike serološkog access SARS-CoV-2 IgG imunohemijskog testa. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
Udruženje farmaceuta Beograd., 20-20.
https://hdl.handle.net/21.15107/rcub_farfar_5590

Sarić-Matutinović M, Milinković N, Dmitrašinović G, Ignjatović S. Dijagnostičke karakteristike serološkog access SARS-CoV-2 IgG imunohemijskog testa. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd. 2022;:20-20.
https://hdl.handle.net/21.15107/rcub_farfar_5590 .

Sarić-Matutinović, Marija, Milinković, Neda, Dmitrašinović, Gordana, Ignjatović, Svetlana, "Dijagnostičke karakteristike serološkog access SARS-CoV-2 IgG imunohemijskog testa" in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd (2022):20-20,
https://hdl.handle.net/21.15107/rcub_farfar_5590 .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Dmitrašinović, Gordana
AU  - Ignjatović, Svetlana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4242
AB  - Previous research suggests that point-of-care (POCT) determination of glycated hemoglobin (HbA1c) is a diagnostic test that can be an adequate alternative to measuring HbA1c in the laboratory. The main goal of this study was to examine the analytical characteristics of the novel INCLIX POCT method for HbA1c determination in order to test its performance before introducing this method into routine use. HbA1c is measured in a duplicate in 44 EDTA blood samples parallel on INCLIX POCT device (Sugitech, Inc.) and using automated turbidimetric immunoinhibition test on Olympus AU400 (Beckman Coulter). The within run imprecision was 7.58%, between runs imprecision was 6.63% and 6.22%, and day-to-day imprecision was 8.80% and 7.51%. Total laboratory imprecision was in agreement with those stated by the manufacturer. A statistically significant Pearson correlation coefficient was calculated (r = 0.871, P < 0.01; linear R2 = 0.757). Using Deming regression analysis, the following equation was obtained: y = - 1.80 + 1.304x. Our results indicate statistically significant correlation, linear relationship, and a significant degree of compatibility between the two analyzed methods. However, the negative bias of the HbA1c values determined on the POCT analyzer compared to the Olympus AU400 was confirmed, highlighting the need to standardize the INCLIX method.
AB  - Prethodna istraživanja sugerišu da je „point-of-care“ metoda (POCT) za određivanje glikoziliranog hemoglobina (HbA1c) dijagnostički test koji može biti adekvatna alternativa merenju HbA1c u laboratoriji. Glavni cilj ovog istraživanja bio je da se ispitaju analitičke karakteristike nove INCLIX POCT metode za određivanje HbA1c kako bi se testirale karakteristike izvođenja pre uvođenja ove metode u rutinsku upotrebu. HbA1c je meren u duplikatu u 44 uzorka EDTA krvi paralelno na INCLIX POCT uređaju (Sugitech, Inc.) i automatskim turbidimetrijskim imunoinhibicijskim testom na Olympus AU400 biohemijskom analizatoru (Beckman Coulter). Nepreciznost u seriji bila je 7,58%, nepreciznost između serija bila je 6,63% i 6,22%, a nepreciznost iz dana u dan bila je 8,80% i 7,51%. Ukupna laboratorijska nepreciznost bila je u slaganju sa podacima koje je naveo proizvođač. Izračunat je statistički značajan Pearsonov koeficijent korelacije (r = 0,871, P < 0,01; linearni R2 = 0,757). Demingovom regresionom analizom dobijena je sledeća jednačina: y = -1,80 + 1,304x. Naši rezultati ukazuju na statistički značajnu korelaciju, linearnu povezanost i značajan stepen kompatibilnosti između dve analizirane metode. Međutim, potvrđen je negativan bias vrednosti HbA1c određenih na POCT analizatoru u odnosu na Olympus AU400, što ističe potrebu za standardizacijom INCLIX metode.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Compliance of the POCT method with the fully automated method for HbA1c determination
T1  - Usklađenost POCT metode sa potpuno automatizovanom metodom za određivanje HbA1c
VL  - 72
IS  - 1
SP  - 77
EP  - 90
DO  - 10.5937/arhfarm72-35905
ER  - 

@article{
author = "Milinković, Neda and Sarić-Matutinović, Marija and Dmitrašinović, Gordana and Ignjatović, Svetlana",
year = "2022",
abstract = "Previous research suggests that point-of-care (POCT) determination of glycated hemoglobin (HbA1c) is a diagnostic test that can be an adequate alternative to measuring HbA1c in the laboratory. The main goal of this study was to examine the analytical characteristics of the novel INCLIX POCT method for HbA1c determination in order to test its performance before introducing this method into routine use. HbA1c is measured in a duplicate in 44 EDTA blood samples parallel on INCLIX POCT device (Sugitech, Inc.) and using automated turbidimetric immunoinhibition test on Olympus AU400 (Beckman Coulter). The within run imprecision was 7.58%, between runs imprecision was 6.63% and 6.22%, and day-to-day imprecision was 8.80% and 7.51%. Total laboratory imprecision was in agreement with those stated by the manufacturer. A statistically significant Pearson correlation coefficient was calculated (r = 0.871, P < 0.01; linear R2 = 0.757). Using Deming regression analysis, the following equation was obtained: y = - 1.80 + 1.304x. Our results indicate statistically significant correlation, linear relationship, and a significant degree of compatibility between the two analyzed methods. However, the negative bias of the HbA1c values determined on the POCT analyzer compared to the Olympus AU400 was confirmed, highlighting the need to standardize the INCLIX method., Prethodna istraživanja sugerišu da je „point-of-care“ metoda (POCT) za određivanje glikoziliranog hemoglobina (HbA1c) dijagnostički test koji može biti adekvatna alternativa merenju HbA1c u laboratoriji. Glavni cilj ovog istraživanja bio je da se ispitaju analitičke karakteristike nove INCLIX POCT metode za određivanje HbA1c kako bi se testirale karakteristike izvođenja pre uvođenja ove metode u rutinsku upotrebu. HbA1c je meren u duplikatu u 44 uzorka EDTA krvi paralelno na INCLIX POCT uređaju (Sugitech, Inc.) i automatskim turbidimetrijskim imunoinhibicijskim testom na Olympus AU400 biohemijskom analizatoru (Beckman Coulter). Nepreciznost u seriji bila je 7,58%, nepreciznost između serija bila je 6,63% i 6,22%, a nepreciznost iz dana u dan bila je 8,80% i 7,51%. Ukupna laboratorijska nepreciznost bila je u slaganju sa podacima koje je naveo proizvođač. Izračunat je statistički značajan Pearsonov koeficijent korelacije (r = 0,871, P < 0,01; linearni R2 = 0,757). Demingovom regresionom analizom dobijena je sledeća jednačina: y = -1,80 + 1,304x. Naši rezultati ukazuju na statistički značajnu korelaciju, linearnu povezanost i značajan stepen kompatibilnosti između dve analizirane metode. Međutim, potvrđen je negativan bias vrednosti HbA1c određenih na POCT analizatoru u odnosu na Olympus AU400, što ističe potrebu za standardizacijom INCLIX metode.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Compliance of the POCT method with the fully automated method for HbA1c determination, Usklađenost POCT metode sa potpuno automatizovanom metodom za određivanje HbA1c",
volume = "72",
number = "1",
pages = "77-90",
doi = "10.5937/arhfarm72-35905"
}

Milinković, N., Sarić-Matutinović, M., Dmitrašinović, G.,& Ignjatović, S.. (2022). Compliance of the POCT method with the fully automated method for HbA1c determination. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 72(1), 77-90.
https://doi.org/10.5937/arhfarm72-35905

Milinković N, Sarić-Matutinović M, Dmitrašinović G, Ignjatović S. Compliance of the POCT method with the fully automated method for HbA1c determination. in Arhiv za farmaciju. 2022;72(1):77-90.
doi:10.5937/arhfarm72-35905 .

Milinković, Neda, Sarić-Matutinović, Marija, Dmitrašinović, Gordana, Ignjatović, Svetlana, "Compliance of the POCT method with the fully automated method for HbA1c determination" in Arhiv za farmaciju, 72, no. 1 (2022):77-90,
https://doi.org/10.5937/arhfarm72-35905 . .

TY  - JOUR
AU  - Mirjanić-Azarić, Bosa
AU  - Milinković, Neda
AU  - Bogavac-Stanojević, Nataša
AU  - Avram, Sanja
AU  - Stojaković-Jelisavac, Tanja
AU  - Stojanović, Darja
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4173
AB  - Background: The aim of this study was to determine the reference intervals (RIs) for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3) and FT3/FT4 ratio using indirect methods. Methods: We analyzed 1256 results TSH, FT4 and FT3 collected from a laboratory information system between 2017 and 2021. All measurements were performed on a Siemens ADVIA Centaur XP analyzer using the chemiluminescent immunoassay. We calculated the values of the 2.5th and 97.5th percentiles as recommended by the IFCC (CLSI C28-A3). Results: The RIs derived for TSH, FT4, FT3 and FT3/FT4 ratio were 0.34–4.10 mIU/L, 11.3–20.6 pmol/L, 3.5–6.32 pmol/L and 0.21–0.47, respectively. We found a significant difference between calculated RIs for the TSH and FT4 and those recommended by the manufacturer. Also, FT3 values were significantly higher in the group younger than 30 years relative to the fourth decade (5.26 vs. 5.02, p=0.005), the fifth decade (5.26 vs. 4.94, p=0.001), the sixth decade (5.26 vs. 4.87, p<0.001), the seventh decade (5.26 vs. 4.79, p<0.001) and the group older than 70 years old (5.26 vs. 4.55, p<0.001). Likewise, we found for TSH values and FT3/FT4 ratio a significant difference (p <0.001) between different age groups. Conclusions: The establishing RIs for the population of the Republic of Srpska were significantly differed from the recommended RIs by the manufacturer for TSH and FT4. Our results encourage other laboratories to develop their own RIs for thyroid parameters by applying CLSI recommendations.
AB  - Uvod: Cilj ove studije bio je da se odrede referentni intervali (RI) tireotropnog hormona (TSH), slobodnog tiroksina (FT4), slobodnog trijodotironina (FT3) i odnosa FT3/FT4 indirektnom metodom procene referentnih intervala. Metode: Analizirali smo 1256 dobijenih vrednosti TSH, FT4 i FT3 u periodu između 2017. i 2021. godine. Rezultate smo uzeli iz laboratorijskog informacionog sistema. Sva merenja su izvedena na Siemens ADVIA Centaur XP analizatoru pomo}u hemiluminiscentnih imunohemijskih testova. Izračunali smo vrednosti 2,5-og i 97,5-og percentila prema preporuci IFCC-a (CLSI C28-A3). Rezultati: Procenjeni RI za TSH, FT4, FT3 i odnos FT3/FT4 bili su 0,34-4,10 mIU/L; 11,3-20,6 pmol/L; 3,5-6,32 pmol/L i 0,21-0,47. Utvrdili smo značajnu razliku između izračunatih RI za TSH i FT4 i onih koje preporučuje proizvođač. Takođe, vrednosti FT3 bile su značajno ve}e u grupi mlađoj od 30 godina u odnosu na četvrtu deceniju (5,26 vs. 5,02; p = 0,005), petu deceniju (5,26 vs. 4,94; p = 0,001), šestu deceniju (5,26 vs. 4,87; p<0,001), sedmu deceniju (5,26 vs. 4,79; p<0,001) i grupu stariju od 70 godina (5,26 vs. 4,55; p<0,001). Isto tako, za vrednosti TSH i odnos FT3/FT4 pronašli smo značajnu razliku (p <0,001) između različitih dobnih grupa. Zaključak: Procenjene vrednosti referentnih intervala za TSH i FT4 za stanovništvo Republike Srpske značajno su se razlikovale od preporučenih RI od strane proizvođača. Naši rezultati podstiču druge laboratorije da izrade sopstvene RI za parametre štitne žlezde primenom CLSI preporuka.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Indirect estimation of reference intervals for thyroid parameters using advia centaur XP analyzer
T1  - Indirektna procjena referentnih intervala za parametre štitne žlijezde upotrebom advia centaur XP analizatora
VL  - 41
IS  - 2
SP  - 238
EP  - 245
DO  - 10.5937/jomb0-33543
ER  - 

@article{
author = "Mirjanić-Azarić, Bosa and Milinković, Neda and Bogavac-Stanojević, Nataša and Avram, Sanja and Stojaković-Jelisavac, Tanja and Stojanović, Darja",
year = "2022",
abstract = "Background: The aim of this study was to determine the reference intervals (RIs) for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3) and FT3/FT4 ratio using indirect methods. Methods: We analyzed 1256 results TSH, FT4 and FT3 collected from a laboratory information system between 2017 and 2021. All measurements were performed on a Siemens ADVIA Centaur XP analyzer using the chemiluminescent immunoassay. We calculated the values of the 2.5th and 97.5th percentiles as recommended by the IFCC (CLSI C28-A3). Results: The RIs derived for TSH, FT4, FT3 and FT3/FT4 ratio were 0.34–4.10 mIU/L, 11.3–20.6 pmol/L, 3.5–6.32 pmol/L and 0.21–0.47, respectively. We found a significant difference between calculated RIs for the TSH and FT4 and those recommended by the manufacturer. Also, FT3 values were significantly higher in the group younger than 30 years relative to the fourth decade (5.26 vs. 5.02, p=0.005), the fifth decade (5.26 vs. 4.94, p=0.001), the sixth decade (5.26 vs. 4.87, p<0.001), the seventh decade (5.26 vs. 4.79, p<0.001) and the group older than 70 years old (5.26 vs. 4.55, p<0.001). Likewise, we found for TSH values and FT3/FT4 ratio a significant difference (p <0.001) between different age groups. Conclusions: The establishing RIs for the population of the Republic of Srpska were significantly differed from the recommended RIs by the manufacturer for TSH and FT4. Our results encourage other laboratories to develop their own RIs for thyroid parameters by applying CLSI recommendations., Uvod: Cilj ove studije bio je da se odrede referentni intervali (RI) tireotropnog hormona (TSH), slobodnog tiroksina (FT4), slobodnog trijodotironina (FT3) i odnosa FT3/FT4 indirektnom metodom procene referentnih intervala. Metode: Analizirali smo 1256 dobijenih vrednosti TSH, FT4 i FT3 u periodu između 2017. i 2021. godine. Rezultate smo uzeli iz laboratorijskog informacionog sistema. Sva merenja su izvedena na Siemens ADVIA Centaur XP analizatoru pomo}u hemiluminiscentnih imunohemijskih testova. Izračunali smo vrednosti 2,5-og i 97,5-og percentila prema preporuci IFCC-a (CLSI C28-A3). Rezultati: Procenjeni RI za TSH, FT4, FT3 i odnos FT3/FT4 bili su 0,34-4,10 mIU/L; 11,3-20,6 pmol/L; 3,5-6,32 pmol/L i 0,21-0,47. Utvrdili smo značajnu razliku između izračunatih RI za TSH i FT4 i onih koje preporučuje proizvođač. Takođe, vrednosti FT3 bile su značajno ve}e u grupi mlađoj od 30 godina u odnosu na četvrtu deceniju (5,26 vs. 5,02; p = 0,005), petu deceniju (5,26 vs. 4,94; p = 0,001), šestu deceniju (5,26 vs. 4,87; p<0,001), sedmu deceniju (5,26 vs. 4,79; p<0,001) i grupu stariju od 70 godina (5,26 vs. 4,55; p<0,001). Isto tako, za vrednosti TSH i odnos FT3/FT4 pronašli smo značajnu razliku (p <0,001) između različitih dobnih grupa. Zaključak: Procenjene vrednosti referentnih intervala za TSH i FT4 za stanovništvo Republike Srpske značajno su se razlikovale od preporučenih RI od strane proizvođača. Naši rezultati podstiču druge laboratorije da izrade sopstvene RI za parametre štitne žlezde primenom CLSI preporuka.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Indirect estimation of reference intervals for thyroid parameters using advia centaur XP analyzer, Indirektna procjena referentnih intervala za parametre štitne žlijezde upotrebom advia centaur XP analizatora",
volume = "41",
number = "2",
pages = "238-245",
doi = "10.5937/jomb0-33543"
}

Mirjanić-Azarić, B., Milinković, N., Bogavac-Stanojević, N., Avram, S., Stojaković-Jelisavac, T.,& Stojanović, D.. (2022). Indirect estimation of reference intervals for thyroid parameters using advia centaur XP analyzer. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(2), 238-245.
https://doi.org/10.5937/jomb0-33543

Mirjanić-Azarić B, Milinković N, Bogavac-Stanojević N, Avram S, Stojaković-Jelisavac T, Stojanović D. Indirect estimation of reference intervals for thyroid parameters using advia centaur XP analyzer. in Journal of Medical Biochemistry. 2022;41(2):238-245.
doi:10.5937/jomb0-33543 .

Mirjanić-Azarić, Bosa, Milinković, Neda, Bogavac-Stanojević, Nataša, Avram, Sanja, Stojaković-Jelisavac, Tanja, Stojanović, Darja, "Indirect estimation of reference intervals for thyroid parameters using advia centaur XP analyzer" in Journal of Medical Biochemistry, 41, no. 2 (2022):238-245,
https://doi.org/10.5937/jomb0-33543 . .

TY  - JOUR
AU  - Dudašova Petrovičova, Olina
AU  - Stanković, Ivan
AU  - Milinković, Neda
AU  - Dopsaj, Violeta
AU  - Đorđević, Brižita
AU  - Dopsaj, Milivoj
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4302
AB  - This study aimed to investigate the effect of supplementation with plant origin superoxide dismutase (SOD), GliSODin, on parameters of muscle damage, metabolic, and work performance at international level rowers. Twenty-eight rowers were included in a randomized, double-blind study. The study was conducted during a 6-week preparation period. At the beginning of the study and after 6 weeks of the supplementation period, all rowers were tested on a rowing ergometer. Blood samples were taken from the antecubital vein before and after every ergometer testing. Muscle damage markers creatine kinase (CK) and lactate dehydrogenase (LDH), total antioxidant capacity (TAC), inflammation parameters interleukin-6 (IL-6), and C-reactive protein (CRP) were measured. Rowing performance was assessed by lactate level in capillary blood and power output on the rowing ergometer. After supplementation, experimental group had significantly lower CK (p = 0.049) and IL-6 (p = 0.035) before and IL-6 (p = 0.050) after exhausting exercise on ergometer. Relative change of power output at 4 mmol/L concentration of lactate in blood, considering the initial and final test, was significantly higher (p = 0.020) in the supplemented group. It was concluded that GliSODin could be considered a good supplement in preventing some deleterious effects of intensive physical activity, including inflammation and muscle damage, and consequently, to enable a better rowing performance of elite rowers.
PB  - MDPI
T2  - Biology
T1  - Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort
VL  - 11
IS  - 10
DO  - 10.3390/biology11101437
ER  - 

@article{
author = "Dudašova Petrovičova, Olina and Stanković, Ivan and Milinković, Neda and Dopsaj, Violeta and Đorđević, Brižita and Dopsaj, Milivoj",
year = "2022",
abstract = "This study aimed to investigate the effect of supplementation with plant origin superoxide dismutase (SOD), GliSODin, on parameters of muscle damage, metabolic, and work performance at international level rowers. Twenty-eight rowers were included in a randomized, double-blind study. The study was conducted during a 6-week preparation period. At the beginning of the study and after 6 weeks of the supplementation period, all rowers were tested on a rowing ergometer. Blood samples were taken from the antecubital vein before and after every ergometer testing. Muscle damage markers creatine kinase (CK) and lactate dehydrogenase (LDH), total antioxidant capacity (TAC), inflammation parameters interleukin-6 (IL-6), and C-reactive protein (CRP) were measured. Rowing performance was assessed by lactate level in capillary blood and power output on the rowing ergometer. After supplementation, experimental group had significantly lower CK (p = 0.049) and IL-6 (p = 0.035) before and IL-6 (p = 0.050) after exhausting exercise on ergometer. Relative change of power output at 4 mmol/L concentration of lactate in blood, considering the initial and final test, was significantly higher (p = 0.020) in the supplemented group. It was concluded that GliSODin could be considered a good supplement in preventing some deleterious effects of intensive physical activity, including inflammation and muscle damage, and consequently, to enable a better rowing performance of elite rowers.",
publisher = "MDPI",
journal = "Biology",
title = "Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort",
volume = "11",
number = "10",
doi = "10.3390/biology11101437"
}

Dudašova Petrovičova, O., Stanković, I., Milinković, N., Dopsaj, V., Đorđević, B.,& Dopsaj, M.. (2022). Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort. in Biology
MDPI., 11(10).
https://doi.org/10.3390/biology11101437

Dudašova Petrovičova O, Stanković I, Milinković N, Dopsaj V, Đorđević B, Dopsaj M. Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort. in Biology. 2022;11(10).
doi:10.3390/biology11101437 .

Dudašova Petrovičova, Olina, Stanković, Ivan, Milinković, Neda, Dopsaj, Violeta, Đorđević, Brižita, Dopsaj, Milivoj, "Effects of 6-Week Supplementation with GliSODin on Parameters of Muscle Damages, Metabolic, and Work Performance at International Level Rowers after Specific Maximal Effort" in Biology, 11, no. 10 (2022),
https://doi.org/10.3390/biology11101437 . .

TY  - JOUR
AU  - Van Schrojenstein Lantman, Marith
AU  - Çubukçu, Hikmet Can
AU  - Boursier, Guilaine
AU  - Panteghini, Mauro
AU  - Bernabeu-Andreu, Francisco
AU  - Milinković, Neda
AU  - Mesko Brguljan, Pika
AU  - Linko, Solveig
AU  - Brugnoni, Duilio
AU  - O'Kelly, Ruth
AU  - Kroupis, Christos
AU  - Lohmander, Maria
AU  - Šprongl, Luděk
AU  - Vanstapel, Florent
AU  - Thelen, Marc
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4072
AB  - Clinicians trust medical laboratories to provide reliable results on which they rely for clinical decisions. Lab- oratories fulfil their responsibility for accurate and consistent results by utilizing an arsenal of approaches, ranging from validation and verification experiments to daily quality control procedures. All these procedures verify, on different moments, that the results of a certain examination procedure have analytical performance characteristics (APC) that meet analytical performance specifications (APS) set for a particular intended use. The APC can in part be determined by esti- mating the measurement uncertainty component under con- ditions of within-laboratory precision (uRw), which comprises all components influencing the measurement uncertainty of random sources. To maintain the adequacy of their mea- surement procedures, laboratories need to distinguish aspects that are manageable vs. those that are not. One of the aspects that may influence uRw is the momentary significant bias caused by shifts in reagent and/or calibrator lots, which, when accepted or unnoticed, become a factor of the APC. In this paper, we postulate a model for allocating a part of allowable uRw to between-reagent lot variation, based on the need for long-term consistency of the measurement variability for that specific measurand. The allocation manages the ratio between short-term and long-term variation and indicates laboratories when to reject or correct certain variations due to reagent lots.
PB  - De Gruyter Open Ltd
T2  - Clinical Chemistry and Laboratory Medicine
T1  - An approach for determining allowable between reagent lot variation
VL  - 60
IS  - 5
SP  - 681
EP  - 688
DO  - 10.1515/cclm-2022-0083
ER  - 

@article{
author = "Van Schrojenstein Lantman, Marith and Çubukçu, Hikmet Can and Boursier, Guilaine and Panteghini, Mauro and Bernabeu-Andreu, Francisco and Milinković, Neda and Mesko Brguljan, Pika and Linko, Solveig and Brugnoni, Duilio and O'Kelly, Ruth and Kroupis, Christos and Lohmander, Maria and Šprongl, Luděk and Vanstapel, Florent and Thelen, Marc",
year = "2022",
abstract = "Clinicians trust medical laboratories to provide reliable results on which they rely for clinical decisions. Lab- oratories fulfil their responsibility for accurate and consistent results by utilizing an arsenal of approaches, ranging from validation and verification experiments to daily quality control procedures. All these procedures verify, on different moments, that the results of a certain examination procedure have analytical performance characteristics (APC) that meet analytical performance specifications (APS) set for a particular intended use. The APC can in part be determined by esti- mating the measurement uncertainty component under con- ditions of within-laboratory precision (uRw), which comprises all components influencing the measurement uncertainty of random sources. To maintain the adequacy of their mea- surement procedures, laboratories need to distinguish aspects that are manageable vs. those that are not. One of the aspects that may influence uRw is the momentary significant bias caused by shifts in reagent and/or calibrator lots, which, when accepted or unnoticed, become a factor of the APC. In this paper, we postulate a model for allocating a part of allowable uRw to between-reagent lot variation, based on the need for long-term consistency of the measurement variability for that specific measurand. The allocation manages the ratio between short-term and long-term variation and indicates laboratories when to reject or correct certain variations due to reagent lots.",
publisher = "De Gruyter Open Ltd",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "An approach for determining allowable between reagent lot variation",
volume = "60",
number = "5",
pages = "681-688",
doi = "10.1515/cclm-2022-0083"
}

Van Schrojenstein Lantman, M., Çubukçu, H. C., Boursier, G., Panteghini, M., Bernabeu-Andreu, F., Milinković, N., Mesko Brguljan, P., Linko, S., Brugnoni, D., O'Kelly, R., Kroupis, C., Lohmander, M., Šprongl, L., Vanstapel, F.,& Thelen, M.. (2022). An approach for determining allowable between reagent lot variation. in Clinical Chemistry and Laboratory Medicine
De Gruyter Open Ltd., 60(5), 681-688.
https://doi.org/10.1515/cclm-2022-0083

Van Schrojenstein Lantman M, Çubukçu HC, Boursier G, Panteghini M, Bernabeu-Andreu F, Milinković N, Mesko Brguljan P, Linko S, Brugnoni D, O'Kelly R, Kroupis C, Lohmander M, Šprongl L, Vanstapel F, Thelen M. An approach for determining allowable between reagent lot variation. in Clinical Chemistry and Laboratory Medicine. 2022;60(5):681-688.
doi:10.1515/cclm-2022-0083 .

Van Schrojenstein Lantman, Marith, Çubukçu, Hikmet Can, Boursier, Guilaine, Panteghini, Mauro, Bernabeu-Andreu, Francisco, Milinković, Neda, Mesko Brguljan, Pika, Linko, Solveig, Brugnoni, Duilio, O'Kelly, Ruth, Kroupis, Christos, Lohmander, Maria, Šprongl, Luděk, Vanstapel, Florent, Thelen, Marc, "An approach for determining allowable between reagent lot variation" in Clinical Chemistry and Laboratory Medicine, 60, no. 5 (2022):681-688,
https://doi.org/10.1515/cclm-2022-0083 . .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Radosavljević, Branimir
AU  - Zeković, Milica
AU  - Korčok, Davor
AU  - Ignjatović, Svetlana
AU  - Đorđević, Brižita
AU  - Milinković, Neda
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4048
AB  - This study aimed to investigate the short-term effects of three magnesium (Mg) dietary supplements containing mineral immediately available for absorption on Mg biochemical status indices (ionized and total Mg), as well as their effects on electrolytes levels in healthy female young adults (n = 61). After a 10-days intervention period supplementation with powder/granulate containing Mg oxide led to an increase in both ionized Mg concentration and % in total Mg in comparison with the baseline. Supplementation with Mg citrate was associated with the significant increase in % of ionized fraction and decrease in serum total Mg concentration. By contrast, among participants consuming Mg carbonate in the form of effervescent tablets ionized Mg concentration and % in total Mg decreased, without detectable changes in serum total Mg. In conclusion, after the short-term supplementation period, Mg oxide demonstrated superior bioavailability compared to the other examined Mg supplements without affecting other minerals’ levels.
PB  - Springer Nature B.V.
T2  - BioMetals
T1  - Effects of short-term magnesium supplementation on ionized, total magnesium and other relevant electrolytes levels
VL  - 35
SP  - 267
EP  - 283
DO  - 10.1007/s10534-022-00363-y
ER  - 

@article{
author = "Ivanović, Nevena and Radosavljević, Branimir and Zeković, Milica and Korčok, Davor and Ignjatović, Svetlana and Đorđević, Brižita and Milinković, Neda",
year = "2022",
abstract = "This study aimed to investigate the short-term effects of three magnesium (Mg) dietary supplements containing mineral immediately available for absorption on Mg biochemical status indices (ionized and total Mg), as well as their effects on electrolytes levels in healthy female young adults (n = 61). After a 10-days intervention period supplementation with powder/granulate containing Mg oxide led to an increase in both ionized Mg concentration and % in total Mg in comparison with the baseline. Supplementation with Mg citrate was associated with the significant increase in % of ionized fraction and decrease in serum total Mg concentration. By contrast, among participants consuming Mg carbonate in the form of effervescent tablets ionized Mg concentration and % in total Mg decreased, without detectable changes in serum total Mg. In conclusion, after the short-term supplementation period, Mg oxide demonstrated superior bioavailability compared to the other examined Mg supplements without affecting other minerals’ levels.",
publisher = "Springer Nature B.V.",
journal = "BioMetals",
title = "Effects of short-term magnesium supplementation on ionized, total magnesium and other relevant electrolytes levels",
volume = "35",
pages = "267-283",
doi = "10.1007/s10534-022-00363-y"
}

Ivanović, N., Radosavljević, B., Zeković, M., Korčok, D., Ignjatović, S., Đorđević, B.,& Milinković, N.. (2022). Effects of short-term magnesium supplementation on ionized, total magnesium and other relevant electrolytes levels. in BioMetals
Springer Nature B.V.., 35, 267-283.
https://doi.org/10.1007/s10534-022-00363-y

Ivanović N, Radosavljević B, Zeković M, Korčok D, Ignjatović S, Đorđević B, Milinković N. Effects of short-term magnesium supplementation on ionized, total magnesium and other relevant electrolytes levels. in BioMetals. 2022;35:267-283.
doi:10.1007/s10534-022-00363-y .

Ivanović, Nevena, Radosavljević, Branimir, Zeković, Milica, Korčok, Davor, Ignjatović, Svetlana, Đorđević, Brižita, Milinković, Neda, "Effects of short-term magnesium supplementation on ionized, total magnesium and other relevant electrolytes levels" in BioMetals, 35 (2022):267-283,
https://doi.org/10.1007/s10534-022-00363-y . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Zeković, Milica
AU  - Dodevska, Margarita
AU  - Đorđević, Brižita
AU  - Radosavljević, Branimir
AU  - Ignjatović, Svetlana
AU  - Ivanović, Nevena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4256
AB  - Background: Literature data indicate the benefit of magne-
sium (Mg) supplementation. The aim of this study was to
examine the effect of short-term Mg supplementation on
iron status in healthy female participants.
Methods: One hundred healthy female students of the
University of Belgrade - Faculty of Pharmacy participated the
study during eleven intervention days. Students ingested Mg
preparations with the same dose of the active substance. The
analysis included the measurement of serum iron, unsaturat-
ed iron binding capacity (UIBC), total iron binding capacity
(TIBC), total Mg (tMg), ionized Mg (iMg), complete blood
count, met-, carboxy- and oxy-haemoglobin (metHgb,
COHgb, O2Hgb). Transferrin concentrations and percentage
of transferrin saturation (SAT) were calculated manually. The
association among the analyzed biochemical parameters was
examined using polynomial regression. A principal compo-
nent analysis (PCA) was used for the evaluation of interde-
pendence between the analyzed parameters.
Results: A statistically significant trend for change in O2Hgb
(%) by tertiles of iMg concentrations was found (P = 0.029).
Serum tMg reached significant positive correlation with the
SAT at concentration levels greater than 0.9 mmol/L, after
11 days of intervention (R2=0.116). Ionized Mg in a con-
centration higher than 0.6 mmol/L is positively correlated
with SAT and serum Fe (R2=0.214; 0.199, respectively).
PCA revealed variability of 64.7% for two axes after 11 days.
Conclusions: Mg supplementation leads to an improvement
in the certain iron status parameters even in individuals with
optimal levels of these indices. However, caution should be
exercised when supplementing Mg, and laboratory monitor-
ing of the interaction is required.
AB  - Uvod: Literaturni podaci ukazuju na benefit suplementacije magnezijumom (Mg). Cilj ove studije bio je da se ispita uticaj kratkotrajne suplementacije Mg na status gvožđa kod zdravih žena. Metode: Sto zdravih studentkinja Univerzitet u Beogradu - Farmaceutskog fakulteta je učestvovalo u istraživanju tokom jedanaest dana intervencije. Studenti su uzimali preparate Mg sa istom dozom aktivne supstance. U serumu je određivano gvožđe, nezasićen kapacitet vezivanja gvožđa (UIBC), ukupan kapacitet vezivanja gvožđa (TIBC), ukupan Mg (tMg), jonizovni Mg (iMg), kompletna krvna slika, met-, karboksiioksihemoglobin (metHgb, COHgb, O2Hgb). Transferin i saturacija transferina (SAT) su izračunati ručno. Povezanost analiziranih biohemijskih parametara je ispitana pomoću polinomalne regresije. Za procenu međuzavisnosti između analiziranih parametara korišćena je analiza glavnih komponenti (PCA). Rezultati: Utvrđen je statistički značajan trend promene O2Hgb (%) po tertilima koncentracija iMg (P = 0,029). Ukupan Mg je dostigao značajnu pozitivnu korelaciju sa SAT pri koncentracijama većim od 0,9 mmol/L, nakon 11 dana intervencije (R2 = 0,116). Jonizovani Mg u koncentraciji većoj od 0,6 mmol/L pozitivno korelira sa SAT i gvožđem (R2 = 0,214; 0,199, redom). PCA analizom je pokazana varijabilnost od 64,7% za dve ose nakon 11 dana. Zaključak: Suplementacija Mg dovodi do poboljšanja određenih parametara statusa gvožđa čak i kod pojedinaca sa optimalnim nivoima ovih parametara. Međutim treba biti oprezan pri suplementaciji Mg, a dodatno je neophodno i laboratorijsko praćenje ovih interakcija.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Magnesium supplementation and iron status among female students: the intervention study
T1  - Suplementacija magnezijuma i status gvožđa kod studentkinja - studija intervencije
VL  - 41
IS  - 3
SP  - 316
EP  - 326
DO  - 10.5937/jomb0-33898
ER  - 

@article{
author = "Milinković, Neda and Zeković, Milica and Dodevska, Margarita and Đorđević, Brižita and Radosavljević, Branimir and Ignjatović, Svetlana and Ivanović, Nevena",
year = "2022",
abstract = "Background: Literature data indicate the benefit of magne-
sium (Mg) supplementation. The aim of this study was to
examine the effect of short-term Mg supplementation on
iron status in healthy female participants.
Methods: One hundred healthy female students of the
University of Belgrade - Faculty of Pharmacy participated the
study during eleven intervention days. Students ingested Mg
preparations with the same dose of the active substance. The
analysis included the measurement of serum iron, unsaturat-
ed iron binding capacity (UIBC), total iron binding capacity
(TIBC), total Mg (tMg), ionized Mg (iMg), complete blood
count, met-, carboxy- and oxy-haemoglobin (metHgb,
COHgb, O2Hgb). Transferrin concentrations and percentage
of transferrin saturation (SAT) were calculated manually. The
association among the analyzed biochemical parameters was
examined using polynomial regression. A principal compo-
nent analysis (PCA) was used for the evaluation of interde-
pendence between the analyzed parameters.
Results: A statistically significant trend for change in O2Hgb
(%) by tertiles of iMg concentrations was found (P = 0.029).
Serum tMg reached significant positive correlation with the
SAT at concentration levels greater than 0.9 mmol/L, after
11 days of intervention (R2=0.116). Ionized Mg in a con-
centration higher than 0.6 mmol/L is positively correlated
with SAT and serum Fe (R2=0.214; 0.199, respectively).
PCA revealed variability of 64.7% for two axes after 11 days.
Conclusions: Mg supplementation leads to an improvement
in the certain iron status parameters even in individuals with
optimal levels of these indices. However, caution should be
exercised when supplementing Mg, and laboratory monitor-
ing of the interaction is required., Uvod: Literaturni podaci ukazuju na benefit suplementacije magnezijumom (Mg). Cilj ove studije bio je da se ispita uticaj kratkotrajne suplementacije Mg na status gvožđa kod zdravih žena. Metode: Sto zdravih studentkinja Univerzitet u Beogradu - Farmaceutskog fakulteta je učestvovalo u istraživanju tokom jedanaest dana intervencije. Studenti su uzimali preparate Mg sa istom dozom aktivne supstance. U serumu je određivano gvožđe, nezasićen kapacitet vezivanja gvožđa (UIBC), ukupan kapacitet vezivanja gvožđa (TIBC), ukupan Mg (tMg), jonizovni Mg (iMg), kompletna krvna slika, met-, karboksiioksihemoglobin (metHgb, COHgb, O2Hgb). Transferin i saturacija transferina (SAT) su izračunati ručno. Povezanost analiziranih biohemijskih parametara je ispitana pomoću polinomalne regresije. Za procenu međuzavisnosti između analiziranih parametara korišćena je analiza glavnih komponenti (PCA). Rezultati: Utvrđen je statistički značajan trend promene O2Hgb (%) po tertilima koncentracija iMg (P = 0,029). Ukupan Mg je dostigao značajnu pozitivnu korelaciju sa SAT pri koncentracijama većim od 0,9 mmol/L, nakon 11 dana intervencije (R2 = 0,116). Jonizovani Mg u koncentraciji većoj od 0,6 mmol/L pozitivno korelira sa SAT i gvožđem (R2 = 0,214; 0,199, redom). PCA analizom je pokazana varijabilnost od 64,7% za dve ose nakon 11 dana. Zaključak: Suplementacija Mg dovodi do poboljšanja određenih parametara statusa gvožđa čak i kod pojedinaca sa optimalnim nivoima ovih parametara. Međutim treba biti oprezan pri suplementaciji Mg, a dodatno je neophodno i laboratorijsko praćenje ovih interakcija.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Magnesium supplementation and iron status among female students: the intervention study, Suplementacija magnezijuma i status gvožđa kod studentkinja - studija intervencije",
volume = "41",
number = "3",
pages = "316-326",
doi = "10.5937/jomb0-33898"
}

Milinković, N., Zeković, M., Dodevska, M., Đorđević, B., Radosavljević, B., Ignjatović, S.,& Ivanović, N.. (2022). Magnesium supplementation and iron status among female students: the intervention study. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 41(3), 316-326.
https://doi.org/10.5937/jomb0-33898

Milinković N, Zeković M, Dodevska M, Đorđević B, Radosavljević B, Ignjatović S, Ivanović N. Magnesium supplementation and iron status among female students: the intervention study. in Journal of Medical Biochemistry. 2022;41(3):316-326.
doi:10.5937/jomb0-33898 .

Milinković, Neda, Zeković, Milica, Dodevska, Margarita, Đorđević, Brižita, Radosavljević, Branimir, Ignjatović, Svetlana, Ivanović, Nevena, "Magnesium supplementation and iron status among female students: the intervention study" in Journal of Medical Biochemistry, 41, no. 3 (2022):316-326,
https://doi.org/10.5937/jomb0-33898 . .

TY  - CONF
AU  - Veličković, Ksenija
AU  - Okuka, Nina
AU  - Polovina, Snežana
AU  - Sumarac‐Dumanović, Mirjana
AU  - Ivanović, Nevena
AU  - Milinković, Neda
AU  - Đorđević, Brižita
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4590
AB  - Obesity could be associated with enhanced platelet (PL) function and thrombosis.
Also, changes in gut microbiota composition were observed in obese patients, and probiotics
supplementation has been shown to improve some of the obesity-associated metabolic
parameters. This study aimed to determine the possible effects of probiotic supplementation
on platelet morphology in overweight/obese premenopausal women.Twenty overweight
(BMI = 25.0-29.9 kg/m2) and obese (BMI ≥ 30.0 kg/m2) women participated in double-
blind, randomized, placebo-controlled study. They were randomly assigned to receive one
capsule daily of probiotics (7x1010 CFU Lactobacillus plantarum 299v (DSM9843), 5x109
CFU Saccharomyces cerevisiae var. boulardii and 40mg octacosanol; N=10) or placebo
(N=10) for 3 months. To determine changes in the parameters of platelet activation - the
platelet count (PC), platelet distribution width (PDW), Feret diameter, and hypogranular
appearance, blood samples were obtained for an automated hematology analyser and
peripheral smears staining. Although difference in PC was not found between groups,
supplementation with probiotics decreased PDW compared to the placebo group (p<0.05).
Regarding platelets diameter, there is an decrease in group supplemented with probiotics
compared to placebo, however, a statistical difference has not been reached. Further, the
proportion of hypogranular PL was increased in a probiotic-supplemented group compared
to the placebo group (p<0.01). In conclusion, probiotic supplementation achieved
improvements in platelets morphology and hematological indices in overweight/obese
premenopausal women.
AB  - Gojaznost je često povezana sa povećanom funkcijom krvnih pločica i trombozom.
Takođe, istraživanja ukazuju na promenjen sastav mikrobioma creva kod gojaznih osoba,
kao i da suplementacija određenim probioticima može imati pozitivan uticaj na telesnu
masu. Cilj rada je bio da se ispita uticaj suplementacije probiotskog preparata na morfološke
karakteristike krvnih pločica u randomizovanoj, dvostruko-slepoj, placebo-kontrolisanoj
studiji. U tu svrhu, regrutovano je 20 žena sa prekomernom težinom (BMI ≥ 25,0 kg/m2) koje
su podeljene u dve grupe. Prva grupa je pila dnevno jednu kapsulu probiotskog preparata
tokom tri meseca (kombinaciju soja Lactobacillus plantarum 299v (DSM9843),
Saccharomyces cerevisiae var. boulardii i 40 mg oktakozanola; N = 10), dok je druga grupa
bila placebo grupa. Određen je broj krvnih pločica, i njihova raspodela po volumenu (eng.
platelet distribution width, PDW), a zatim je posmatrana morfologija krvnih pločica i
izmeren njihov dijametar iz krvnih razmaza. Analiza je pokazala da su krvne pločice kod
gojaznih/žena sa prekomernom težinom uglavnom krupne, što je jedan od pokazatelja
njihove aktivnosti. U interventoj grupi nije zapažena promena u broju krvnih pločica, ali je
značajno smanjen PDW u poređenju sa placebo grupom (p < 0,05). Dalje, pokazano je da je
udeo hipogranularnih krvnih pločica bio povećan u poređenju sa placebo grupom (p < 0,01).
Ovi rezultati ukazuju na pozitivan efekat probiotika na morfološke karakteristike krvnih
pločica i mogućnost primene probiotika u regulaciji njihove aktivnosti u patološkim
stanjima.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Effects of probiotic supplementation on platelet morphology in overweight/obese woman
T1  - Efekti suplementacije probiotikom na morfologiju krvnih pločica gojaznih žena i žena sa prokomernom težinom
VL  - 72
IS  - 4 suplement
SP  - S509
EP  - S510
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4590
ER  - 

@conference{
author = "Veličković, Ksenija and Okuka, Nina and Polovina, Snežana and Sumarac‐Dumanović, Mirjana and Ivanović, Nevena and Milinković, Neda and Đorđević, Brižita",
year = "2022",
abstract = "Obesity could be associated with enhanced platelet (PL) function and thrombosis.
Also, changes in gut microbiota composition were observed in obese patients, and probiotics
supplementation has been shown to improve some of the obesity-associated metabolic
parameters. This study aimed to determine the possible effects of probiotic supplementation
on platelet morphology in overweight/obese premenopausal women.Twenty overweight
(BMI = 25.0-29.9 kg/m2) and obese (BMI ≥ 30.0 kg/m2) women participated in double-
blind, randomized, placebo-controlled study. They were randomly assigned to receive one
capsule daily of probiotics (7x1010 CFU Lactobacillus plantarum 299v (DSM9843), 5x109
CFU Saccharomyces cerevisiae var. boulardii and 40mg octacosanol; N=10) or placebo
(N=10) for 3 months. To determine changes in the parameters of platelet activation - the
platelet count (PC), platelet distribution width (PDW), Feret diameter, and hypogranular
appearance, blood samples were obtained for an automated hematology analyser and
peripheral smears staining. Although difference in PC was not found between groups,
supplementation with probiotics decreased PDW compared to the placebo group (p<0.05).
Regarding platelets diameter, there is an decrease in group supplemented with probiotics
compared to placebo, however, a statistical difference has not been reached. Further, the
proportion of hypogranular PL was increased in a probiotic-supplemented group compared
to the placebo group (p<0.01). In conclusion, probiotic supplementation achieved
improvements in platelets morphology and hematological indices in overweight/obese
premenopausal women., Gojaznost je često povezana sa povećanom funkcijom krvnih pločica i trombozom.
Takođe, istraživanja ukazuju na promenjen sastav mikrobioma creva kod gojaznih osoba,
kao i da suplementacija određenim probioticima može imati pozitivan uticaj na telesnu
masu. Cilj rada je bio da se ispita uticaj suplementacije probiotskog preparata na morfološke
karakteristike krvnih pločica u randomizovanoj, dvostruko-slepoj, placebo-kontrolisanoj
studiji. U tu svrhu, regrutovano je 20 žena sa prekomernom težinom (BMI ≥ 25,0 kg/m2) koje
su podeljene u dve grupe. Prva grupa je pila dnevno jednu kapsulu probiotskog preparata
tokom tri meseca (kombinaciju soja Lactobacillus plantarum 299v (DSM9843),
Saccharomyces cerevisiae var. boulardii i 40 mg oktakozanola; N = 10), dok je druga grupa
bila placebo grupa. Određen je broj krvnih pločica, i njihova raspodela po volumenu (eng.
platelet distribution width, PDW), a zatim je posmatrana morfologija krvnih pločica i
izmeren njihov dijametar iz krvnih razmaza. Analiza je pokazala da su krvne pločice kod
gojaznih/žena sa prekomernom težinom uglavnom krupne, što je jedan od pokazatelja
njihove aktivnosti. U interventoj grupi nije zapažena promena u broju krvnih pločica, ali je
značajno smanjen PDW u poređenju sa placebo grupom (p < 0,05). Dalje, pokazano je da je
udeo hipogranularnih krvnih pločica bio povećan u poređenju sa placebo grupom (p < 0,01).
Ovi rezultati ukazuju na pozitivan efekat probiotika na morfološke karakteristike krvnih
pločica i mogućnost primene probiotika u regulaciji njihove aktivnosti u patološkim
stanjima.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Effects of probiotic supplementation on platelet morphology in overweight/obese woman, Efekti suplementacije probiotikom na morfologiju krvnih pločica gojaznih žena i žena sa prokomernom težinom",
volume = "72",
number = "4 suplement",
pages = "S509-S510",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4590"
}

Veličković, K., Okuka, N., Polovina, S., Sumarac‐Dumanović, M., Ivanović, N., Milinković, N.,& Đorđević, B.. (2022). Effects of probiotic supplementation on platelet morphology in overweight/obese woman. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S509-S510.
https://hdl.handle.net/21.15107/rcub_farfar_4590

Veličković K, Okuka N, Polovina S, Sumarac‐Dumanović M, Ivanović N, Milinković N, Đorđević B. Effects of probiotic supplementation on platelet morphology in overweight/obese woman. in Arhiv za farmaciju. 2022;72(4 suplement):S509-S510.
https://hdl.handle.net/21.15107/rcub_farfar_4590 .

Veličković, Ksenija, Okuka, Nina, Polovina, Snežana, Sumarac‐Dumanović, Mirjana, Ivanović, Nevena, Milinković, Neda, Đorđević, Brižita, "Effects of probiotic supplementation on platelet morphology in overweight/obese woman" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S509-S510,
https://hdl.handle.net/21.15107/rcub_farfar_4590 .

TY  - JOUR
AU  - Milinković, Neda
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4183
AB  - Under the auspices of Society of Medical
Biochemists of Serbia (SMBS), the 16th NTK –
Accredited Laboratories Conference was held on
September 08, 2021. This Conference was traditionally held within International Conventions on Quality
in the organization of the United Association of
Serbia for Quality (UASQ), Belgrade, Serbia.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - 16th NTK - Accredited Laboratories Conference-Society of Medical Biochemists of Serbia International Conventions on Quality in the organization of the United Association of Serbia for Quality (UASQ), Belgrade, Serbia
VL  - 41
IS  - 2
SP  - 246
EP  - 247
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4183
ER  - 

@article{
author = "Milinković, Neda",
year = "2022",
abstract = "Under the auspices of Society of Medical
Biochemists of Serbia (SMBS), the 16th NTK –
Accredited Laboratories Conference was held on
September 08, 2021. This Conference was traditionally held within International Conventions on Quality
in the organization of the United Association of
Serbia for Quality (UASQ), Belgrade, Serbia.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "16th NTK - Accredited Laboratories Conference-Society of Medical Biochemists of Serbia International Conventions on Quality in the organization of the United Association of Serbia for Quality (UASQ), Belgrade, Serbia",
volume = "41",
number = "2",
pages = "246-247",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4183"
}

Milinković, N.. (2022). 16th NTK - Accredited Laboratories Conference-Society of Medical Biochemists of Serbia International Conventions on Quality in the organization of the United Association of Serbia for Quality (UASQ), Belgrade, Serbia. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(2), 246-247.
https://hdl.handle.net/21.15107/rcub_farfar_4183

Milinković N. 16th NTK - Accredited Laboratories Conference-Society of Medical Biochemists of Serbia International Conventions on Quality in the organization of the United Association of Serbia for Quality (UASQ), Belgrade, Serbia. in Journal of Medical Biochemistry. 2022;41(2):246-247.
https://hdl.handle.net/21.15107/rcub_farfar_4183 .

Milinković, Neda, "16th NTK - Accredited Laboratories Conference-Society of Medical Biochemists of Serbia International Conventions on Quality in the organization of the United Association of Serbia for Quality (UASQ), Belgrade, Serbia" in Journal of Medical Biochemistry, 41, no. 2 (2022):246-247,
https://hdl.handle.net/21.15107/rcub_farfar_4183 .

TY  - CONF
AU  - Prvulović, Marija
AU  - Ivanović, Nevena
AU  - Zeković, Milica
AU  - Milinković, Neda
AU  - Dodevska, Margarita
AU  - Kukić-Marković, Jelena
AU  - Okuka, Nina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5093
AB  - Nuts are nutrient-dense foods with complex matrices rich in unsaturated fatty acids and other bioactive compounds. Numerous reported health benefits of nuts may be attributed to their unique nutrient profiles and synergistic interaction of their constituents, including macronutrients, micronutrients and phytochemicals. The objective of this study was to explore pharmacy students’ nuts consumption practice, as well as their attitudes and knowledge concerning nuts nutritional and health properties. A cross-sectional survey based on the online self-administered questionnaire, was conducted in September 2020. Among respondents (n=136, 90.4% female and 9.6% male, mean age 23.7±2.0 years), more than half agreed or strongly agreed that regular consumption of nuts might have beneficial effects on health, including reducing the risk of cardiovascular disease. Furthermore, the majority of participants perceived nuts as healthy sources of proteins and monounsaturated and polyunsaturated fats. The most common reason cited by respondents for eating nuts were taste (80.2%), followed by health effects (53.1%) and nutrient profiles (45.0%). The most commonly consumed nuts among pharmacy students were almonds (35.2%) and peanuts (28.4%), followed by hazelnuts (19.1%) and walnuts (15.6%). Based on self-reported data, students predominantly consume raw nuts (54.0%) as a snack between meals (78.5%). Nutrition-related courses within the faculty curriculum were the main sources of acquiring information regarding the beneficial effects nuts exert on health outcomes. Although certain gaps in knowledge were determined, in most senior students, the attitudes and dietary practices regarding the consumption of nuts were at an adequate level. Nevertheless, expanded efforts and additional educational programs may be useful in strengthening the capacities of these future health professionals for providing adequate nutrition advice and proactive advocacy of nuts health benefits.
PB  - University of Belgrade
C3  - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
T1  - Knowledge, attitudes and dietary practices of pharmacy students regarding the importance of nuts consumption
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5093
ER  - 

@conference{
author = "Prvulović, Marija and Ivanović, Nevena and Zeković, Milica and Milinković, Neda and Dodevska, Margarita and Kukić-Marković, Jelena and Okuka, Nina",
year = "2021",
abstract = "Nuts are nutrient-dense foods with complex matrices rich in unsaturated fatty acids and other bioactive compounds. Numerous reported health benefits of nuts may be attributed to their unique nutrient profiles and synergistic interaction of their constituents, including macronutrients, micronutrients and phytochemicals. The objective of this study was to explore pharmacy students’ nuts consumption practice, as well as their attitudes and knowledge concerning nuts nutritional and health properties. A cross-sectional survey based on the online self-administered questionnaire, was conducted in September 2020. Among respondents (n=136, 90.4% female and 9.6% male, mean age 23.7±2.0 years), more than half agreed or strongly agreed that regular consumption of nuts might have beneficial effects on health, including reducing the risk of cardiovascular disease. Furthermore, the majority of participants perceived nuts as healthy sources of proteins and monounsaturated and polyunsaturated fats. The most common reason cited by respondents for eating nuts were taste (80.2%), followed by health effects (53.1%) and nutrient profiles (45.0%). The most commonly consumed nuts among pharmacy students were almonds (35.2%) and peanuts (28.4%), followed by hazelnuts (19.1%) and walnuts (15.6%). Based on self-reported data, students predominantly consume raw nuts (54.0%) as a snack between meals (78.5%). Nutrition-related courses within the faculty curriculum were the main sources of acquiring information regarding the beneficial effects nuts exert on health outcomes. Although certain gaps in knowledge were determined, in most senior students, the attitudes and dietary practices regarding the consumption of nuts were at an adequate level. Nevertheless, expanded efforts and additional educational programs may be useful in strengthening the capacities of these future health professionals for providing adequate nutrition advice and proactive advocacy of nuts health benefits.",
publisher = "University of Belgrade",
journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts",
title = "Knowledge, attitudes and dietary practices of pharmacy students regarding the importance of nuts consumption",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5093"
}

Prvulović, M., Ivanović, N., Zeković, M., Milinković, N., Dodevska, M., Kukić-Marković, J.,& Okuka, N.. (2021). Knowledge, attitudes and dietary practices of pharmacy students regarding the importance of nuts consumption. in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
University of Belgrade., 88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5093

Prvulović M, Ivanović N, Zeković M, Milinković N, Dodevska M, Kukić-Marković J, Okuka N. Knowledge, attitudes and dietary practices of pharmacy students regarding the importance of nuts consumption. in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5093 .

Prvulović, Marija, Ivanović, Nevena, Zeković, Milica, Milinković, Neda, Dodevska, Margarita, Kukić-Marković, Jelena, Okuka, Nina, "Knowledge, attitudes and dietary practices of pharmacy students regarding the importance of nuts consumption" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):88-88,
https://hdl.handle.net/21.15107/rcub_farfar_5093 .

TY  - CONF
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4076
AB  - In medical laboratories, bias presents quantitative expression of trueness, as
analytical performance characteristic that describes systematic errors. Despite bias evaluation
is an integral part of basic lessons of laboratory quality management, it is still difficult to achieve
adequate bias estimation in routine laboratory practice. There are several principles that could
be used to estimate bias, but fundamental for evaluating bias in routine medical laboratories is
the availability of a suitable reference materials. Besides, it is important that the measuring
method and measuring analytical system are in a stable state, and that they have completed
an appropriate validation or verification process. This could be checked routinely by the results
of internal and/or external quality control. In addition, information about the uncertainty of the
certified reference material is a prerequisite for testing the bias. Significant efforts were made
to minimized bias in routine laboratory practice, using reference measurement methods and
commutable reference materials. Still, there are some challenges that remain for the laboratory
professionals as the end users, due to remaining bias between measurement methods and
systems from different and/or the same manufacturers.
PB  - Beograd : JUSK - Jedinstveno Udruženje Srbije za Kvalitet
C3  - XVII međunarodna konvencija o kvalitetu - Zbornik radova JUSK ICQ 2021, 9-11 jun Beograd
T1  - Bias in medical biochemistry- what laboratory professionals need to know?
SP  - 56
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4076
ER  - 

@conference{
author = "Milinković, Neda and Ignjatović, Svetlana",
year = "2021",
abstract = "In medical laboratories, bias presents quantitative expression of trueness, as
analytical performance characteristic that describes systematic errors. Despite bias evaluation
is an integral part of basic lessons of laboratory quality management, it is still difficult to achieve
adequate bias estimation in routine laboratory practice. There are several principles that could
be used to estimate bias, but fundamental for evaluating bias in routine medical laboratories is
the availability of a suitable reference materials. Besides, it is important that the measuring
method and measuring analytical system are in a stable state, and that they have completed
an appropriate validation or verification process. This could be checked routinely by the results
of internal and/or external quality control. In addition, information about the uncertainty of the
certified reference material is a prerequisite for testing the bias. Significant efforts were made
to minimized bias in routine laboratory practice, using reference measurement methods and
commutable reference materials. Still, there are some challenges that remain for the laboratory
professionals as the end users, due to remaining bias between measurement methods and
systems from different and/or the same manufacturers.",
publisher = "Beograd : JUSK - Jedinstveno Udruženje Srbije za Kvalitet",
journal = "XVII međunarodna konvencija o kvalitetu - Zbornik radova JUSK ICQ 2021, 9-11 jun Beograd",
title = "Bias in medical biochemistry- what laboratory professionals need to know?",
pages = "56-63",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4076"
}

Milinković, N.,& Ignjatović, S.. (2021). Bias in medical biochemistry- what laboratory professionals need to know?. in XVII međunarodna konvencija o kvalitetu - Zbornik radova JUSK ICQ 2021, 9-11 jun Beograd
Beograd : JUSK - Jedinstveno Udruženje Srbije za Kvalitet., 56-63.
https://hdl.handle.net/21.15107/rcub_farfar_4076

Milinković N, Ignjatović S. Bias in medical biochemistry- what laboratory professionals need to know?. in XVII međunarodna konvencija o kvalitetu - Zbornik radova JUSK ICQ 2021, 9-11 jun Beograd. 2021;:56-63.
https://hdl.handle.net/21.15107/rcub_farfar_4076 .

Milinković, Neda, Ignjatović, Svetlana, "Bias in medical biochemistry- what laboratory professionals need to know?" in XVII međunarodna konvencija o kvalitetu - Zbornik radova JUSK ICQ 2021, 9-11 jun Beograd (2021):56-63,
https://hdl.handle.net/21.15107/rcub_farfar_4076 .

TY  - JOUR
AU  - Çubukçu, Hikmet Can
AU  - Vanstapel, Florent
AU  - Thelen, Marc
AU  - Bernabeu-Andreu, Francisco A.
AU  - Van Schrojenstein Lantman, Marith
AU  - Brugnoni, Duilio
AU  - Mesko Brguljan, Pika
AU  - Milinković, Neda
AU  - Linko, Solveig
AU  - Vaubourdolle, Michel
AU  - O'Kelly, Ruth
AU  - Kroupis, Christos
AU  - Lohmander, Maria
AU  - Šprongl, Luděk
AU  - Panteghini, Mauro
AU  - Boursier, Guilaine
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4077
AB  - The ISO 15189:2012 standard section 5.9.1 requires laboratories to review results before release, considering quality control, previous results, and clinical information, if any, and to issue documented procedures about it. While laboratory result reporting is generally regarded as part of the post-analytical phase, the result release process requires a general view of the total examination process. Reviewing test results may follow with troubleshooting and test repetition, including reanalyzing an individual sample or resampling. A systematic understanding of the result release may help laboratory professionals carry out appropriate test repetition and ensure the plausibility of laboratory results. In this paper, we addressed the crucial steps in the result release process, including evaluation of sample quality, critical result notification, result reporting, and recommendations for the management of the result release, considering quality control alerts, instrument flags, warning messages, and interference indexes. Error detection tools and plausibility checks mentioned in the present paper can support the daily practice of results release.
PB  - Elsevier
T2  - Clinica Chimica Acta
T1  - Improving the laboratory result release process in the light of ISO 15189:2012 standard
VL  - 522
SP  - 167
EP  - 173
DO  - 10.1016/j.cca.2021.08.013
ER  - 

@article{
author = "Çubukçu, Hikmet Can and Vanstapel, Florent and Thelen, Marc and Bernabeu-Andreu, Francisco A. and Van Schrojenstein Lantman, Marith and Brugnoni, Duilio and Mesko Brguljan, Pika and Milinković, Neda and Linko, Solveig and Vaubourdolle, Michel and O'Kelly, Ruth and Kroupis, Christos and Lohmander, Maria and Šprongl, Luděk and Panteghini, Mauro and Boursier, Guilaine",
year = "2021",
abstract = "The ISO 15189:2012 standard section 5.9.1 requires laboratories to review results before release, considering quality control, previous results, and clinical information, if any, and to issue documented procedures about it. While laboratory result reporting is generally regarded as part of the post-analytical phase, the result release process requires a general view of the total examination process. Reviewing test results may follow with troubleshooting and test repetition, including reanalyzing an individual sample or resampling. A systematic understanding of the result release may help laboratory professionals carry out appropriate test repetition and ensure the plausibility of laboratory results. In this paper, we addressed the crucial steps in the result release process, including evaluation of sample quality, critical result notification, result reporting, and recommendations for the management of the result release, considering quality control alerts, instrument flags, warning messages, and interference indexes. Error detection tools and plausibility checks mentioned in the present paper can support the daily practice of results release.",
publisher = "Elsevier",
journal = "Clinica Chimica Acta",
title = "Improving the laboratory result release process in the light of ISO 15189:2012 standard",
volume = "522",
pages = "167-173",
doi = "10.1016/j.cca.2021.08.013"
}

Çubukçu, H. C., Vanstapel, F., Thelen, M., Bernabeu-Andreu, F. A., Van Schrojenstein Lantman, M., Brugnoni, D., Mesko Brguljan, P., Milinković, N., Linko, S., Vaubourdolle, M., O'Kelly, R., Kroupis, C., Lohmander, M., Šprongl, L., Panteghini, M.,& Boursier, G.. (2021). Improving the laboratory result release process in the light of ISO 15189:2012 standard. in Clinica Chimica Acta
Elsevier., 522, 167-173.
https://doi.org/10.1016/j.cca.2021.08.013

Çubukçu HC, Vanstapel F, Thelen M, Bernabeu-Andreu FA, Van Schrojenstein Lantman M, Brugnoni D, Mesko Brguljan P, Milinković N, Linko S, Vaubourdolle M, O'Kelly R, Kroupis C, Lohmander M, Šprongl L, Panteghini M, Boursier G. Improving the laboratory result release process in the light of ISO 15189:2012 standard. in Clinica Chimica Acta. 2021;522:167-173.
doi:10.1016/j.cca.2021.08.013 .

Çubukçu, Hikmet Can, Vanstapel, Florent, Thelen, Marc, Bernabeu-Andreu, Francisco A., Van Schrojenstein Lantman, Marith, Brugnoni, Duilio, Mesko Brguljan, Pika, Milinković, Neda, Linko, Solveig, Vaubourdolle, Michel, O'Kelly, Ruth, Kroupis, Christos, Lohmander, Maria, Šprongl, Luděk, Panteghini, Mauro, Boursier, Guilaine, "Improving the laboratory result release process in the light of ISO 15189:2012 standard" in Clinica Chimica Acta, 522 (2021):167-173,
https://doi.org/10.1016/j.cca.2021.08.013 . .

TY  - JOUR
AU  - Milovanović, Vera
AU  - Topić, Aleksandra
AU  - Milinković, Neda
AU  - Lazić, Zorica
AU  - Ivošević, Anita
AU  - Radojković, Dragica
AU  - Divac Rankov, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4003
AB  - Objective: Chronic obstructive pulmonary disease (COPD) is multi-factorial disorder which
results from environmental influences and genetic factors. We aimed to investigate whether
methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with
COPD development and severity in Serbian adult population.
Methods: The study included 155 patients with COPD and 134 healthy volunteers. Genotyping
was determined performing home-made polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxi-
dized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxi-
dized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was
presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).
Results: Frequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients
and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in geno-
type or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was signifi-
cantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-
Elastase) was significantly lower in COPD patients than in the control group. In COPD group,
increased level of OxyA1ATwas present in G allele carriers who were smokers relative to G allele
carriers who were not smokers. In the smoker group of patients with severe and very severe
COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.
Conclusion: These findings suggest that MSRA rs10903323 gene polymorphism is probably not a
risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized
A1AT in COPD-smokers, and in severe form of COPD.
PB  - Elsevier
T2  - Pulmonology
T1  - Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients
DO  - 10.1016/j.pulmoe.2021.09.003
ER  - 

@article{
author = "Milovanović, Vera and Topić, Aleksandra and Milinković, Neda and Lazić, Zorica and Ivošević, Anita and Radojković, Dragica and Divac Rankov, Aleksandra",
year = "2021",
abstract = "Objective: Chronic obstructive pulmonary disease (COPD) is multi-factorial disorder which
results from environmental influences and genetic factors. We aimed to investigate whether
methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with
COPD development and severity in Serbian adult population.
Methods: The study included 155 patients with COPD and 134 healthy volunteers. Genotyping
was determined performing home-made polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxi-
dized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxi-
dized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was
presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).
Results: Frequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients
and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in geno-
type or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was signifi-
cantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-
Elastase) was significantly lower in COPD patients than in the control group. In COPD group,
increased level of OxyA1ATwas present in G allele carriers who were smokers relative to G allele
carriers who were not smokers. In the smoker group of patients with severe and very severe
COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.
Conclusion: These findings suggest that MSRA rs10903323 gene polymorphism is probably not a
risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized
A1AT in COPD-smokers, and in severe form of COPD.",
publisher = "Elsevier",
journal = "Pulmonology",
title = "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients",
doi = "10.1016/j.pulmoe.2021.09.003"
}

Milovanović, V., Topić, A., Milinković, N., Lazić, Z., Ivošević, A., Radojković, D.,& Divac Rankov, A.. (2021). Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology
Elsevier..
https://doi.org/10.1016/j.pulmoe.2021.09.003

Milovanović V, Topić A, Milinković N, Lazić Z, Ivošević A, Radojković D, Divac Rankov A. Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology. 2021;.
doi:10.1016/j.pulmoe.2021.09.003 .

Milovanović, Vera, Topić, Aleksandra, Milinković, Neda, Lazić, Zorica, Ivošević, Anita, Radojković, Dragica, Divac Rankov, Aleksandra, "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients" in Pulmonology (2021),
https://doi.org/10.1016/j.pulmoe.2021.09.003 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Pejanović, Svetlana
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3617
AB  - Background: Problem  of  the  variability  between  the different  methods  using  for  bone  alkaline  phosphatase(bALP)  determination  greately  influences  the  clinical significance of bALP as direct marker of bone metabolism.The  aim  of  this  study  was  to  compare  immunoassay  with electrophoresis technique for bALP determination.  Methods: We  measured  bALP  in  71  patients  on  hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter).Results: The   analyzed   methods   showed   significant correlation  (Spearman’s  rho:  0.776,  P  <  0.01),  but  we found statistically significant (P < 0.01) positive bias (27%)for  the  results  measured  by  immunoassay.  In  support  of this,  using  electrophoresis  technique  we  have  detected presence  of  the  intestinal  isoenzymes  of  alkaline  phosphatase in 55% of patients with median value of 30% of the total  alkaline  phoshatase  and  presence  of  liver-2  alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant  concordance  between  two  analysed  methods.Cusum  test  showed  no  significant  deviation  from  linearity(P=0.850).Conclusions:Despite  good  agreement  between  immunoassay  methods  and  electrophoresis  technique  for  bALP determination,  interchangeability  between  these  two methods  is  questionable.  Although  immunoassays  are increasingly  used,  as  fully  automated  methods,  in  a  large number  of  laboratories  and  become  routine  methods  for bALP determination, it should be beared in mind, besidesvarious  interferences,  also  the  heterogeneity  of  the  bALP itself, especially in patients on hemodialysis.
AB  - Uvod: Problem varijabilnosti između različitih metoda pomoću kojih se određuje koštani izoezim alkalne fosfataze (bALP) u velikoj meri utiče na klinički značaj bALP kao direktnog markera metabolizma kostiju. Cilj ove studije bio je poređenje imunohemijskog testa sa elektroforetskom tehnikom za određivanje bALP. Metode: bALP je određen kod 71 pacijenta na hemodijalizi, agar gel elektroforetskom tehnikom (ISO-PAL, SEBIA) i imunohemijskom metodom (OSTASE, Beckman Coulter). Rezultati: Analizirane metode pokazale su značajno slaganje (Spearmanov rho: 0,776, P < 0,01), ali je uočen statistički značajan (P < 0,01) pozitivan bias (27%) za rezultate izmerene imunohemijskom metodom. U prilog ovome, primenom elektroforetske tehnike, otkrili smo prisustvo intestinalnih izoenzima alkalne fosfataze u 55% bolesnika, sa srednjom vrednosti od 30% ukupne alkalne fostataze, kao i prisustvo jetrenih izoformi alkalne fosfataze kod 42% bolesnika sa srednjom vrednosti od 16,6%. Kendall-ov W od 0,787 (P < 0,0001) ukazivao je na značajno slaganje između dve analizirane metode. Cusumov test nije pokazao značajno odstupanje od linearnosti (P = 0,850). Zaključak: Uprkos tome što postoji dobro slaganje između imunohemijske metode i elektroforetske tehnike za određivanje bALP-a, međusobna zamenjivost ove dve metode je pod znakom pitanja. Iako se imunohemijske metode sve više koriste kao potpuno automatizovane, u velikom broju laboratorija i postaju rutinske metode za određivanje bALP-a, treba imati na umu, osim različitih interferencija, i heterogenost samog bALP, posebno kod bolesnika na hemodijalizi.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients
T1  - Poređenje imunohemijske metode i elektroforetske tehnike za određivanje koštanog izoenzima alkalne fosfataze kod pacijenata na hemodijalizi
VL  - 39
IS  - 2
SP  - 178
EP  - 183
DO  - 10.2478/jomb-2019-0048
ER  - 

@article{
author = "Milinković, Neda and Sarić-Matutinović, Marija and Pejanović, Svetlana and Ignjatović, Svetlana",
year = "2020",
abstract = "Background: Problem  of  the  variability  between  the different  methods  using  for  bone  alkaline  phosphatase(bALP)  determination  greately  influences  the  clinical significance of bALP as direct marker of bone metabolism.The  aim  of  this  study  was  to  compare  immunoassay  with electrophoresis technique for bALP determination.  Methods: We  measured  bALP  in  71  patients  on  hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter).Results: The   analyzed   methods   showed   significant correlation  (Spearman’s  rho:  0.776,  P  <  0.01),  but  we found statistically significant (P < 0.01) positive bias (27%)for  the  results  measured  by  immunoassay.  In  support  of this,  using  electrophoresis  technique  we  have  detected presence  of  the  intestinal  isoenzymes  of  alkaline  phosphatase in 55% of patients with median value of 30% of the total  alkaline  phoshatase  and  presence  of  liver-2  alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant  concordance  between  two  analysed  methods.Cusum  test  showed  no  significant  deviation  from  linearity(P=0.850).Conclusions:Despite  good  agreement  between  immunoassay  methods  and  electrophoresis  technique  for  bALP determination,  interchangeability  between  these  two methods  is  questionable.  Although  immunoassays  are increasingly  used,  as  fully  automated  methods,  in  a  large number  of  laboratories  and  become  routine  methods  for bALP determination, it should be beared in mind, besidesvarious  interferences,  also  the  heterogeneity  of  the  bALP itself, especially in patients on hemodialysis., Uvod: Problem varijabilnosti između različitih metoda pomoću kojih se određuje koštani izoezim alkalne fosfataze (bALP) u velikoj meri utiče na klinički značaj bALP kao direktnog markera metabolizma kostiju. Cilj ove studije bio je poređenje imunohemijskog testa sa elektroforetskom tehnikom za određivanje bALP. Metode: bALP je određen kod 71 pacijenta na hemodijalizi, agar gel elektroforetskom tehnikom (ISO-PAL, SEBIA) i imunohemijskom metodom (OSTASE, Beckman Coulter). Rezultati: Analizirane metode pokazale su značajno slaganje (Spearmanov rho: 0,776, P < 0,01), ali je uočen statistički značajan (P < 0,01) pozitivan bias (27%) za rezultate izmerene imunohemijskom metodom. U prilog ovome, primenom elektroforetske tehnike, otkrili smo prisustvo intestinalnih izoenzima alkalne fosfataze u 55% bolesnika, sa srednjom vrednosti od 30% ukupne alkalne fostataze, kao i prisustvo jetrenih izoformi alkalne fosfataze kod 42% bolesnika sa srednjom vrednosti od 16,6%. Kendall-ov W od 0,787 (P < 0,0001) ukazivao je na značajno slaganje između dve analizirane metode. Cusumov test nije pokazao značajno odstupanje od linearnosti (P = 0,850). Zaključak: Uprkos tome što postoji dobro slaganje između imunohemijske metode i elektroforetske tehnike za određivanje bALP-a, međusobna zamenjivost ove dve metode je pod znakom pitanja. Iako se imunohemijske metode sve više koriste kao potpuno automatizovane, u velikom broju laboratorija i postaju rutinske metode za određivanje bALP-a, treba imati na umu, osim različitih interferencija, i heterogenost samog bALP, posebno kod bolesnika na hemodijalizi.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients, Poređenje imunohemijske metode i elektroforetske tehnike za određivanje koštanog izoenzima alkalne fosfataze kod pacijenata na hemodijalizi",
volume = "39",
number = "2",
pages = "178-183",
doi = "10.2478/jomb-2019-0048"
}

Milinković, N., Sarić-Matutinović, M., Pejanović, S.,& Ignjatović, S.. (2020). Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(2), 178-183.
https://doi.org/10.2478/jomb-2019-0048

Milinković N, Sarić-Matutinović M, Pejanović S, Ignjatović S. Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients. in Journal of Medical Biochemistry. 2020;39(2):178-183.
doi:10.2478/jomb-2019-0048 .

Milinković, Neda, Sarić-Matutinović, Marija, Pejanović, Svetlana, Ignjatović, Svetlana, "Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients" in Journal of Medical Biochemistry, 39, no. 2 (2020):178-183,
https://doi.org/10.2478/jomb-2019-0048 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3742
AB  - Measurement uncertainty (MU) of results is one of the basic recommended and accepted statistical
methods in laboratory medicine, with which analytical and clinical evaluation of laboratory
test quality is assessed. Literature data indicate that the calculation of MU is not a simple process,
but that its assessment in daily laboratory practice should be reduced to routine and simple
presentation, understandable to both laboratory professionals and physicians. In order to achieve
this, it is necessary to understand the purpose of the test for which MU is to be determined.
Various suggestions have been given for presentation of MU as a quantitative indicator of the
quality of the final measurement result in the medical laboratory. Although MU refers to the final
measurement result, this metrological concept reflects the entire laboratory measurement process.
The data on estimated MU is used to interpret the measured numerical result, and represents
quantitatively the quality of the measurement itself, i.e. how different are the results of
multiple measurements of the analyte of interest in the same sample, as well as whether the
method of determination itself is subjected to significant random and systematic deviation.
Initially, in the metrological concept, the MU is viewed in relation to the true value of the analyte
of interest. However, the true value of the analyte measured in the biological fluid matrix of the
study population cannot be known. It is therefore considered the closest value obtained by the
perfect method, for which the bias and inaccuracy, as measures of systematic and random error,
are equal to zero, which is practically impossible to achieve in routine laboratory practice.
Although current standards require accredited medical laboratories to estimate MU, none of
these guidelines provide clear guidance on how this can be achieved in daily laboratory work.
This review examines literary data and documents dealing with MU issues, but also highlights
what additional terms and data should be considered when interpreting MU. This paper ultimately
draws attention, and once again points out, that a simpler solution is needed for this universal
concept to be formally and universally applicable in routine laboratory medicine practice.
PB  - Taylor & Francis Group
T2  - Critical Reviews in Clinical Laboratory Sciences
T1  - Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?
DO  - 10.1080/10408363.2020.1784838
ER  - 

@article{
author = "Milinković, Neda and Jovičić, Snežana and Ignjatović, Svetlana",
year = "2020",
abstract = "Measurement uncertainty (MU) of results is one of the basic recommended and accepted statistical
methods in laboratory medicine, with which analytical and clinical evaluation of laboratory
test quality is assessed. Literature data indicate that the calculation of MU is not a simple process,
but that its assessment in daily laboratory practice should be reduced to routine and simple
presentation, understandable to both laboratory professionals and physicians. In order to achieve
this, it is necessary to understand the purpose of the test for which MU is to be determined.
Various suggestions have been given for presentation of MU as a quantitative indicator of the
quality of the final measurement result in the medical laboratory. Although MU refers to the final
measurement result, this metrological concept reflects the entire laboratory measurement process.
The data on estimated MU is used to interpret the measured numerical result, and represents
quantitatively the quality of the measurement itself, i.e. how different are the results of
multiple measurements of the analyte of interest in the same sample, as well as whether the
method of determination itself is subjected to significant random and systematic deviation.
Initially, in the metrological concept, the MU is viewed in relation to the true value of the analyte
of interest. However, the true value of the analyte measured in the biological fluid matrix of the
study population cannot be known. It is therefore considered the closest value obtained by the
perfect method, for which the bias and inaccuracy, as measures of systematic and random error,
are equal to zero, which is practically impossible to achieve in routine laboratory practice.
Although current standards require accredited medical laboratories to estimate MU, none of
these guidelines provide clear guidance on how this can be achieved in daily laboratory work.
This review examines literary data and documents dealing with MU issues, but also highlights
what additional terms and data should be considered when interpreting MU. This paper ultimately
draws attention, and once again points out, that a simpler solution is needed for this universal
concept to be formally and universally applicable in routine laboratory medicine practice.",
publisher = "Taylor & Francis Group",
journal = "Critical Reviews in Clinical Laboratory Sciences",
title = "Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?",
doi = "10.1080/10408363.2020.1784838"
}

Milinković, N., Jovičić, S.,& Ignjatović, S.. (2020). Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?. in Critical Reviews in Clinical Laboratory Sciences
Taylor & Francis Group..
https://doi.org/10.1080/10408363.2020.1784838

Milinković N, Jovičić S, Ignjatović S. Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?. in Critical Reviews in Clinical Laboratory Sciences. 2020;.
doi:10.1080/10408363.2020.1784838 .

Milinković, Neda, Jovičić, Snežana, Ignjatović, Svetlana, "Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?" in Critical Reviews in Clinical Laboratory Sciences (2020),
https://doi.org/10.1080/10408363.2020.1784838 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić, Marija
AU  - Jovičić, Snežana
AU  - Mirković, Duško
AU  - Ležaić, Višnja
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3624
AB  - Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients.
AB  - Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients
T1  - Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega
VL  - 39
IS  - 3
SP  - 309
EP  - 317
DO  - 10.2478/jomb-2019-0032
ER  - 

@article{
author = "Milinković, Neda and Sarić, Marija and Jovičić, Snežana and Mirković, Duško and Ležaić, Višnja and Ignjatović, Svetlana",
year = "2020",
abstract = "Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients., Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients, Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega",
volume = "39",
number = "3",
pages = "309-317",
doi = "10.2478/jomb-2019-0032"
}

Milinković, N., Sarić, M., Jovičić, S., Mirković, D., Ležaić, V.,& Ignjatović, S.. (2020). Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 309-317.
https://doi.org/10.2478/jomb-2019-0032

Milinković N, Sarić M, Jovičić S, Mirković D, Ležaić V, Ignjatović S. Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry. 2020;39(3):309-317.
doi:10.2478/jomb-2019-0032 .

Milinković, Neda, Sarić, Marija, Jovičić, Snežana, Mirković, Duško, Ležaić, Višnja, Ignjatović, Svetlana, "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients" in Journal of Medical Biochemistry, 39, no. 3 (2020):309-317,
https://doi.org/10.2478/jomb-2019-0032 . .

TY  - JOUR
AU  - Radović, Ana
AU  - Milinković, Neda
AU  - Stošović, Milan
AU  - Dopsaj, Violeta
AU  - Ignjatović, Svetlana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3566
AB  - Literature data indicate that increased uric acid (UA) levels are an important risk factor for cardiovascular disease. The aim of this study was to examine the association of  UA with cardiovascular risk factors in patients with hemodialysis using retrospective analysis. In 110 patients,  we  studied  the  values  of  basic  renal  function  parameters,  bone  metabolism  and cardiovascular risk factors in the period from 2010 to 2017. The results indicate a significant increase in urea (P=0.004), creatinine (P=0.028) and inorganic phosphate (P=0.001), as well as significant differences in gender (P=0.013), in risk categories defined on the basis of the UA decision  limit  (cut  off  <350  μmol/L).  After  controlling  the  effects  of  most  parameters, statistically  significant  correlation  coefficients  were  obtained  for  UA  and  urea  (r=0.361; P=0.0013),  creatinine  (r=0.388;  P=0.0005)  and  inorganic  phosphate  (r=0.366;  P=0.0011). Significant regression coefficients were obtained for UA and male gender (β=-0.227, P=0.004), age (β=-0.298, P˂0.001), urea (β=0.271, P=0.005) and inorganic phosphate (β=0.232, P=0.009). The  predictive  value  of  independent  parameters  in  relation  to  UA  was  confirmed  for  male gender  (OR=3.595;  95%  CI:  1.421-9.094;  P=0.007)  and  inorganic  phosphate  (OR=14.842; 2.518-87.472, P=0.003). By Cox regression analysis of proportional hazard ratio, we obtained the most significant combined effect of the body mass index, dialysis and diastolic pressure on UA concentration in relation to the duration of hemodialysis (P <0.0001). The results of this long-term  study  suggest  that  UA  can  not  be  considered  an  independent  cardiovascular  risk factor, but that HD patients need to strategically control the level of MK in order to reduce the resulting complications, morbidity and mortality.
AB  - Literaturni  podaci  ukazuju  da  povećana  koncentracija  mokraćne  kiseline  (MK) predstavlja  značajan  faktor  rizika  za  kardiovaskularne  bolesti.  Cilj  ove  studije  bio  je  da  se retrospektivnom analizom ispita povezanost MK sa faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi. Za 110 bolesnika analizirane su vrednosti osnovnih parametara funkcije bubrega, metabolizma kostiju i faktora rizika za kardiovaskularne bolesti u periodu od 2010. do 2017. godine. Rezultati ukazuju na značajno povećanje koncentracije uree (P=0,004), kreatinina (P=0,028) i neorganskog fosfata (P˂0,001), kao i značajne razlike u zavisnosti od pola (P=0,013) u kategorijama rizika definisanim na osnovu granične koncentracije MK (cut off <350  μmol/L).  Nakon  kontrolisanja  efekta  većine  parametara,  statistički  značajne  vrednosti koeficijenata  korelacije  dobijene  su  za  MK  i  ureu  (r=0,361;  P=0,0013),  kreatinin  (r=0,388; P=0,0005) i neorganski fosfat (r=0,366; P=0,0011). Značajni regresioni koeficijenti su dobijeni za MK i muški pol (β=-0,227, P=0,004), godine starosti (β=-0,298, P˂0,001), ureu (β=0,271, P=0,005) i neorganski fosfat (β=0,232, P=0,009). Prediktivna vrednost nezavisnih parametara u odnosu  na  MK  je  potvrđena  za  muški  pol  (OR=3,595;  95%  CI:  1,421-9,094;  P=0,007)  i neorganski  fosfat  (OR=14,842;  95%  CI:  2,518-87,472,  P=0,003).  Cox-ovom  regresijom proporcionalnih  nepoželjnih  ishoda  dobijen  je  najznačajniji  udruženi  efekat  indeksa  telesne mase  i  adekvatnosti  dijalize  na  koncentraciju  MK  u  odnosu  na  dužinu  trajanja  hemodijalize (P<0,0001). Rezultati ove dugoročne studije ukazuju da se MK ne može smatrati nezavisnim faktorom rizika za kardiovaskularne bolesti, ali da je kod bolesnika na HD, potrebno strateški kontrolisati nivo MK u cilju smanjenja posledičnih komplikacija, morbiditeta i mortaliteta.
PB  - Beograd: Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis
T1  - Povezanost koncentracije mokraćne kiseline sa tradicionalnim i ne-tradicionalnim faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi – retrospektivna analiza
VL  - 69
IS  - 5
SP  - 323
EP  - 337
DO  - 10.5937/arhfarm1905323R
ER  - 

@article{
author = "Radović, Ana and Milinković, Neda and Stošović, Milan and Dopsaj, Violeta and Ignjatović, Svetlana",
year = "2019",
abstract = "Literature data indicate that increased uric acid (UA) levels are an important risk factor for cardiovascular disease. The aim of this study was to examine the association of  UA with cardiovascular risk factors in patients with hemodialysis using retrospective analysis. In 110 patients,  we  studied  the  values  of  basic  renal  function  parameters,  bone  metabolism  and cardiovascular risk factors in the period from 2010 to 2017. The results indicate a significant increase in urea (P=0.004), creatinine (P=0.028) and inorganic phosphate (P=0.001), as well as significant differences in gender (P=0.013), in risk categories defined on the basis of the UA decision  limit  (cut  off  <350  μmol/L).  After  controlling  the  effects  of  most  parameters, statistically  significant  correlation  coefficients  were  obtained  for  UA  and  urea  (r=0.361; P=0.0013),  creatinine  (r=0.388;  P=0.0005)  and  inorganic  phosphate  (r=0.366;  P=0.0011). Significant regression coefficients were obtained for UA and male gender (β=-0.227, P=0.004), age (β=-0.298, P˂0.001), urea (β=0.271, P=0.005) and inorganic phosphate (β=0.232, P=0.009). The  predictive  value  of  independent  parameters  in  relation  to  UA  was  confirmed  for  male gender  (OR=3.595;  95%  CI:  1.421-9.094;  P=0.007)  and  inorganic  phosphate  (OR=14.842; 2.518-87.472, P=0.003). By Cox regression analysis of proportional hazard ratio, we obtained the most significant combined effect of the body mass index, dialysis and diastolic pressure on UA concentration in relation to the duration of hemodialysis (P <0.0001). The results of this long-term  study  suggest  that  UA  can  not  be  considered  an  independent  cardiovascular  risk factor, but that HD patients need to strategically control the level of MK in order to reduce the resulting complications, morbidity and mortality., Literaturni  podaci  ukazuju  da  povećana  koncentracija  mokraćne  kiseline  (MK) predstavlja  značajan  faktor  rizika  za  kardiovaskularne  bolesti.  Cilj  ove  studije  bio  je  da  se retrospektivnom analizom ispita povezanost MK sa faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi. Za 110 bolesnika analizirane su vrednosti osnovnih parametara funkcije bubrega, metabolizma kostiju i faktora rizika za kardiovaskularne bolesti u periodu od 2010. do 2017. godine. Rezultati ukazuju na značajno povećanje koncentracije uree (P=0,004), kreatinina (P=0,028) i neorganskog fosfata (P˂0,001), kao i značajne razlike u zavisnosti od pola (P=0,013) u kategorijama rizika definisanim na osnovu granične koncentracije MK (cut off <350  μmol/L).  Nakon  kontrolisanja  efekta  većine  parametara,  statistički  značajne  vrednosti koeficijenata  korelacije  dobijene  su  za  MK  i  ureu  (r=0,361;  P=0,0013),  kreatinin  (r=0,388; P=0,0005) i neorganski fosfat (r=0,366; P=0,0011). Značajni regresioni koeficijenti su dobijeni za MK i muški pol (β=-0,227, P=0,004), godine starosti (β=-0,298, P˂0,001), ureu (β=0,271, P=0,005) i neorganski fosfat (β=0,232, P=0,009). Prediktivna vrednost nezavisnih parametara u odnosu  na  MK  je  potvrđena  za  muški  pol  (OR=3,595;  95%  CI:  1,421-9,094;  P=0,007)  i neorganski  fosfat  (OR=14,842;  95%  CI:  2,518-87,472,  P=0,003).  Cox-ovom  regresijom proporcionalnih  nepoželjnih  ishoda  dobijen  je  najznačajniji  udruženi  efekat  indeksa  telesne mase  i  adekvatnosti  dijalize  na  koncentraciju  MK  u  odnosu  na  dužinu  trajanja  hemodijalize (P<0,0001). Rezultati ove dugoročne studije ukazuju da se MK ne može smatrati nezavisnim faktorom rizika za kardiovaskularne bolesti, ali da je kod bolesnika na HD, potrebno strateški kontrolisati nivo MK u cilju smanjenja posledičnih komplikacija, morbiditeta i mortaliteta.",
publisher = "Beograd: Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis, Povezanost koncentracije mokraćne kiseline sa tradicionalnim i ne-tradicionalnim faktorima rizika za kardiovaskularne bolesti kod bolesnika na hemodijalizi – retrospektivna analiza",
volume = "69",
number = "5",
pages = "323-337",
doi = "10.5937/arhfarm1905323R"
}

Radović, A., Milinković, N., Stošović, M., Dopsaj, V.,& Ignjatović, S.. (2019). The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis. in Arhiv za farmaciju
Beograd: Savez farmaceutskih udruženja Srbije., 69(5), 323-337.
https://doi.org/10.5937/arhfarm1905323R

Radović A, Milinković N, Stošović M, Dopsaj V, Ignjatović S. The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis. in Arhiv za farmaciju. 2019;69(5):323-337.
doi:10.5937/arhfarm1905323R .

Radović, Ana, Milinković, Neda, Stošović, Milan, Dopsaj, Violeta, Ignjatović, Svetlana, "The relationship between uric acid concentration and cardiovascular risk: Retrospective analysis of patients on hemodialysis" in Arhiv za farmaciju, 69, no. 5 (2019):323-337,
https://doi.org/10.5937/arhfarm1905323R . .