TY  - JOUR
AU  - Milošević, Tamara
AU  - Sopić, Miron
AU  - Vekić, Jelena
AU  - Guzonjić, Azra
AU  - Vujčić, Sanja
AU  - Pešić, Snežana
AU  - Miljković-Trailović, Milica
AU  - Naumović, Radomir
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5414
AB  - Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.
PB  - Springer Science and Business Media B.V.
T2  - International Urology and Nephrology
T1  - The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients
VL  - 56
IS  - 2
SP  - 615
EP  - 623
DO  - 10.1007/s11255-023-03696-w
ER  - 

@article{
author = "Milošević, Tamara and Sopić, Miron and Vekić, Jelena and Guzonjić, Azra and Vujčić, Sanja and Pešić, Snežana and Miljković-Trailović, Milica and Naumović, Radomir and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "International Urology and Nephrology",
title = "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients",
volume = "56",
number = "2",
pages = "615-623",
doi = "10.1007/s11255-023-03696-w"
}

Milošević, T., Sopić, M., Vekić, J., Guzonjić, A., Vujčić, S., Pešić, S., Miljković-Trailović, M., Naumović, R.,& Kotur-Stevuljević, J.. (2024). The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology
Springer Science and Business Media B.V.., 56(2), 615-623.
https://doi.org/10.1007/s11255-023-03696-w

Milošević T, Sopić M, Vekić J, Guzonjić A, Vujčić S, Pešić S, Miljković-Trailović M, Naumović R, Kotur-Stevuljević J. The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology. 2024;56(2):615-623.
doi:10.1007/s11255-023-03696-w .

Milošević, Tamara, Sopić, Miron, Vekić, Jelena, Guzonjić, Azra, Vujčić, Sanja, Pešić, Snežana, Miljković-Trailović, Milica, Naumović, Radomir, Kotur-Stevuljević, Jelena, "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients" in International Urology and Nephrology, 56, no. 2 (2024):615-623,
https://doi.org/10.1007/s11255-023-03696-w . .

TY  - JOUR
AU  - Paunović, Marija
AU  - Kotur-Stevuljević, Jelena
AU  - Arsić, Aleksandra
AU  - Milošević, Maja
AU  - Todorović, Vanja
AU  - Guzonjić, Azra
AU  - Vučić, Vesna
AU  - Petrović, Snježana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4918
AB  - A Western-style diet, rich in fat and simple sugars, is the main risk factor for a significant number of chronic diseases and disorders, as well as for a progression of metabolic syndrome (MetS). One of the key mechanisms involved in MetS development is increased oxidative stress caused by the accumulation of body fat. Some dietary polyphenols have shown a protective role in preventing oxidative-stress-induced damage. We investigated the difference in the oxidative response of plasma, liver, and visceral adipose tissue in rats fed with a high-fat high-fructose (HFF) diet for ten weeks, and the effectiveness of polyphenol-rich juices (black currant (BC) and cornelian cherry (CC)) in HFF-diet-induced oxidative stress prevention. The most prominent impact of the HFF diet on redox parameters was recorded in the liver, whereas adipose tissue showed the most potent protection mechanisms against oxidative stress. Consumption of both juices decreased advanced oxidation protein product (AOPP) level in plasma, increased paraoxonase1 (PON1) activity in the liver, and significantly decreased total oxidative status (TOS) in adipose tissue. BC exerted stronger antioxidative potential than CC and decreased the superoxide anion radical (O2•−) level in the liver. It also reduced TOS, total antioxidative status (TAS), and malondialdehyde (MDA) concentration in adipose tissue. The multiple linear regression analysis has shown that the best predictors of MetS development, estimated through the increase in visceral adiposity, were superoxide dismutase (SOD), AOPP, TOS, and TAS. The consumption of polyphenol-rich juices may provide a convenient approach for the systemic reduction of oxidative stress parameters.
PB  - MDPI
T2  - Antioxidants
T1  - Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats
VL  - 12
IS  - 6
DO  - 10.3390/antiox12061148
ER  - 

@article{
author = "Paunović, Marija and Kotur-Stevuljević, Jelena and Arsić, Aleksandra and Milošević, Maja and Todorović, Vanja and Guzonjić, Azra and Vučić, Vesna and Petrović, Snježana",
year = "2023",
abstract = "A Western-style diet, rich in fat and simple sugars, is the main risk factor for a significant number of chronic diseases and disorders, as well as for a progression of metabolic syndrome (MetS). One of the key mechanisms involved in MetS development is increased oxidative stress caused by the accumulation of body fat. Some dietary polyphenols have shown a protective role in preventing oxidative-stress-induced damage. We investigated the difference in the oxidative response of plasma, liver, and visceral adipose tissue in rats fed with a high-fat high-fructose (HFF) diet for ten weeks, and the effectiveness of polyphenol-rich juices (black currant (BC) and cornelian cherry (CC)) in HFF-diet-induced oxidative stress prevention. The most prominent impact of the HFF diet on redox parameters was recorded in the liver, whereas adipose tissue showed the most potent protection mechanisms against oxidative stress. Consumption of both juices decreased advanced oxidation protein product (AOPP) level in plasma, increased paraoxonase1 (PON1) activity in the liver, and significantly decreased total oxidative status (TOS) in adipose tissue. BC exerted stronger antioxidative potential than CC and decreased the superoxide anion radical (O2•−) level in the liver. It also reduced TOS, total antioxidative status (TAS), and malondialdehyde (MDA) concentration in adipose tissue. The multiple linear regression analysis has shown that the best predictors of MetS development, estimated through the increase in visceral adiposity, were superoxide dismutase (SOD), AOPP, TOS, and TAS. The consumption of polyphenol-rich juices may provide a convenient approach for the systemic reduction of oxidative stress parameters.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats",
volume = "12",
number = "6",
doi = "10.3390/antiox12061148"
}

Paunović, M., Kotur-Stevuljević, J., Arsić, A., Milošević, M., Todorović, V., Guzonjić, A., Vučić, V.,& Petrović, S.. (2023). Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats. in Antioxidants
MDPI., 12(6).
https://doi.org/10.3390/antiox12061148

Paunović M, Kotur-Stevuljević J, Arsić A, Milošević M, Todorović V, Guzonjić A, Vučić V, Petrović S. Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats. in Antioxidants. 2023;12(6).
doi:10.3390/antiox12061148 .

Paunović, Marija, Kotur-Stevuljević, Jelena, Arsić, Aleksandra, Milošević, Maja, Todorović, Vanja, Guzonjić, Azra, Vučić, Vesna, Petrović, Snježana, "Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats" in Antioxidants, 12, no. 6 (2023),
https://doi.org/10.3390/antiox12061148 . .

TY  - DATA
AU  - Kotur-Stevuljević, Jelena
AU  - Roksandić-Milenković, Marina
AU  - Dimić, Nemanja
AU  - Dimić, Dejan
AU  - Ćujić, Danica
AU  - Vekić, Jelena
AU  - Guzonjić, Azra
AU  - Gagić, Jelena
AU  - Timotijević, Ljiljana
AU  - Žujović, Dejan
AU  - Gnjatović, Marija
AU  - Todorović, Aleksandra
AU  - Bogavac-Stanojević, Nataša
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5108
AB  - Background A three-year COVID-19 pandemic revealed a spectrum of disease severity and clinical manifestations. The most intriguing part of this phenomenon lays in interindividual variability in COVID-19 course among patients, which is attributable to patient's age, comorbidities and general health status. Focus of this follow-up study was to assess leukocyte telomere length change in mild-to-moderate COVID-19 patients and concomitant influence of inflammation, oxidative stress (OS), pulmonary involvement and implemented therapy on the course of the disease.

Material and methods Routine biochemical/haematological parameters, markers of OS (prooxidants and antioxidants), vitamin D, IgM and IgG antibodies level and relative length of leukocyte telomeres (rLTL) were measured at three time-points (at diagnosis, after 14 and 21 days from the disease onset) in blood samples of 31 consecutive COVID-19 patients, with a mild (n=16) and moderate (n=15) form of the disease, treated on an outpatient basis.

Results Although the patients had reduced rLTL at baseline (median: 0.592; 25th – 75th percentiles: 0.518-0.724), it significantly increased during the follow-up (median: 0.773; 25th – 75th percentiles: 0.615-0.923; P<0.01). The rate of telomere attrition was associated with the extent of OS and pulmonary involvement. During follow-up, the burden of OS was reduced, while antioxidative defence mechanisms were recovered. The use of antibiotics and N-acetylcysteine-propolis supplementation was associated with telomere lengthening.

Conclusion Results of this study revealed significant interaction between OS, inflammation and leukocyte telomere length attrition in COVID-19. Our data suggest that rRTL can be a biomarker that enables more precise therapy decision and accurate patient status estimation.
PB  - Hindawi
T1  - Supplementary material for the article: Kotur-Stevuljević, J.; Roksandić-Milenković, M.; Dimić, N. ...et. al. Leukocyte telomere dynamic change in patients with mild to moderate COVID-19 during three weeks of follow-up: relation with therapy
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5108
ER  - 

@misc{
author = "Kotur-Stevuljević, Jelena and Roksandić-Milenković, Marina and Dimić, Nemanja and Dimić, Dejan and Ćujić, Danica and Vekić, Jelena and Guzonjić, Azra and Gagić, Jelena and Timotijević, Ljiljana and Žujović, Dejan and Gnjatović, Marija and Todorović, Aleksandra and Bogavac-Stanojević, Nataša",
year = "2023",
abstract = "Background A three-year COVID-19 pandemic revealed a spectrum of disease severity and clinical manifestations. The most intriguing part of this phenomenon lays in interindividual variability in COVID-19 course among patients, which is attributable to patient's age, comorbidities and general health status. Focus of this follow-up study was to assess leukocyte telomere length change in mild-to-moderate COVID-19 patients and concomitant influence of inflammation, oxidative stress (OS), pulmonary involvement and implemented therapy on the course of the disease.

Material and methods Routine biochemical/haematological parameters, markers of OS (prooxidants and antioxidants), vitamin D, IgM and IgG antibodies level and relative length of leukocyte telomeres (rLTL) were measured at three time-points (at diagnosis, after 14 and 21 days from the disease onset) in blood samples of 31 consecutive COVID-19 patients, with a mild (n=16) and moderate (n=15) form of the disease, treated on an outpatient basis.

Results Although the patients had reduced rLTL at baseline (median: 0.592; 25th – 75th percentiles: 0.518-0.724), it significantly increased during the follow-up (median: 0.773; 25th – 75th percentiles: 0.615-0.923; P<0.01). The rate of telomere attrition was associated with the extent of OS and pulmonary involvement. During follow-up, the burden of OS was reduced, while antioxidative defence mechanisms were recovered. The use of antibiotics and N-acetylcysteine-propolis supplementation was associated with telomere lengthening.

Conclusion Results of this study revealed significant interaction between OS, inflammation and leukocyte telomere length attrition in COVID-19. Our data suggest that rRTL can be a biomarker that enables more precise therapy decision and accurate patient status estimation.",
publisher = "Hindawi",
title = "Supplementary material for the article: Kotur-Stevuljević, J.; Roksandić-Milenković, M.; Dimić, N. ...et. al. Leukocyte telomere dynamic change in patients with mild to moderate COVID-19 during three weeks of follow-up: relation with therapy",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5108"
}

Kotur-Stevuljević, J., Roksandić-Milenković, M., Dimić, N., Dimić, D., Ćujić, D., Vekić, J., Guzonjić, A., Gagić, J., Timotijević, L., Žujović, D., Gnjatović, M., Todorović, A.,& Bogavac-Stanojević, N.. (2023). Supplementary material for the article: Kotur-Stevuljević, J.; Roksandić-Milenković, M.; Dimić, N. ...et. al. Leukocyte telomere dynamic change in patients with mild to moderate COVID-19 during three weeks of follow-up: relation with therapy. 
Hindawi..
https://hdl.handle.net/21.15107/rcub_farfar_5108

Kotur-Stevuljević J, Roksandić-Milenković M, Dimić N, Dimić D, Ćujić D, Vekić J, Guzonjić A, Gagić J, Timotijević L, Žujović D, Gnjatović M, Todorović A, Bogavac-Stanojević N. Supplementary material for the article: Kotur-Stevuljević, J.; Roksandić-Milenković, M.; Dimić, N. ...et. al. Leukocyte telomere dynamic change in patients with mild to moderate COVID-19 during three weeks of follow-up: relation with therapy. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5108 .

Kotur-Stevuljević, Jelena, Roksandić-Milenković, Marina, Dimić, Nemanja, Dimić, Dejan, Ćujić, Danica, Vekić, Jelena, Guzonjić, Azra, Gagić, Jelena, Timotijević, Ljiljana, Žujović, Dejan, Gnjatović, Marija, Todorović, Aleksandra, Bogavac-Stanojević, Nataša, "Supplementary material for the article: Kotur-Stevuljević, J.; Roksandić-Milenković, M.; Dimić, N. ...et. al. Leukocyte telomere dynamic change in patients with mild to moderate COVID-19 during three weeks of follow-up: relation with therapy" (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5108 .

TY  - JOUR
AU  - Miladinov, Marko
AU  - Rosić, Jovana
AU  - Erić, Katarina
AU  - Guzonjić, Azra
AU  - Jelenković, Jelenko
AU  - Bogavac-Stanojević, Nataša
AU  - Dimitrijević, Ivan
AU  - Kotur-Stevuljević, Jelena
AU  - Barišić, Goran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5204
AB  - The Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune evasion mechanism exploited by different tumors. Additionally, excessive generation of free radicals, redox imbalance, and consequential DNA damage can affect intestinal cell homeostasis and lead to neoplastic transformation. Therefore, our study aimed to investigate the significance of SLFN11 and PD-L1 proteins and redox status parameters as prognostic biomarkers in CRC patients. This study included a total of 155 CRC patients. SLFN11 and PD-L1 serum levels were measured with ELISA and evaluated based on redox status parameters, sociodemographic and clinical characteristics, and survival. The following redox status parameters were investigated: spectrophotometrically measured superoxide dismutase (SOD), sulfhydryl (SH) groups, advanced oxidation protein products (AOPP), malondialdehyde (MDA), pro-oxidant–antioxidant balance (PAB), and superoxide anion (O2•–). The prooxidative score, antioxidative score, and OXY-SCORE were also calculated. The results showed significantly shorter survival in patients with higher OXY-SCOREs and higher levels of serum SLFN11, while only histopathology-analysis-related factors showed significant prognostic value. OXY-SCORE and SLFN11 levels may harbor prognostic potential in CRC patients.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients
VL  - 24
IS  - 20
DO  - 10.3390/ijms242015083
ER  - 

@article{
author = "Miladinov, Marko and Rosić, Jovana and Erić, Katarina and Guzonjić, Azra and Jelenković, Jelenko and Bogavac-Stanojević, Nataša and Dimitrijević, Ivan and Kotur-Stevuljević, Jelena and Barišić, Goran",
year = "2023",
abstract = "The Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune evasion mechanism exploited by different tumors. Additionally, excessive generation of free radicals, redox imbalance, and consequential DNA damage can affect intestinal cell homeostasis and lead to neoplastic transformation. Therefore, our study aimed to investigate the significance of SLFN11 and PD-L1 proteins and redox status parameters as prognostic biomarkers in CRC patients. This study included a total of 155 CRC patients. SLFN11 and PD-L1 serum levels were measured with ELISA and evaluated based on redox status parameters, sociodemographic and clinical characteristics, and survival. The following redox status parameters were investigated: spectrophotometrically measured superoxide dismutase (SOD), sulfhydryl (SH) groups, advanced oxidation protein products (AOPP), malondialdehyde (MDA), pro-oxidant–antioxidant balance (PAB), and superoxide anion (O2•–). The prooxidative score, antioxidative score, and OXY-SCORE were also calculated. The results showed significantly shorter survival in patients with higher OXY-SCOREs and higher levels of serum SLFN11, while only histopathology-analysis-related factors showed significant prognostic value. OXY-SCORE and SLFN11 levels may harbor prognostic potential in CRC patients.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients",
volume = "24",
number = "20",
doi = "10.3390/ijms242015083"
}

Miladinov, M., Rosić, J., Erić, K., Guzonjić, A., Jelenković, J., Bogavac-Stanojević, N., Dimitrijević, I., Kotur-Stevuljević, J.,& Barišić, G.. (2023). Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients. in International Journal of Molecular Sciences
MDPI., 24(20).
https://doi.org/10.3390/ijms242015083

Miladinov M, Rosić J, Erić K, Guzonjić A, Jelenković J, Bogavac-Stanojević N, Dimitrijević I, Kotur-Stevuljević J, Barišić G. Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients. in International Journal of Molecular Sciences. 2023;24(20).
doi:10.3390/ijms242015083 .

Miladinov, Marko, Rosić, Jovana, Erić, Katarina, Guzonjić, Azra, Jelenković, Jelenko, Bogavac-Stanojević, Nataša, Dimitrijević, Ivan, Kotur-Stevuljević, Jelena, Barišić, Goran, "Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients" in International Journal of Molecular Sciences, 24, no. 20 (2023),
https://doi.org/10.3390/ijms242015083 . .

TY  - JOUR
AU  - Belić, Milica
AU  - Sopić, Miron
AU  - Roksandić-Milenković, Marina
AU  - Ćeriman, Vesna
AU  - Guzonjić, Azra
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Dimić, Nemanja
AU  - Jovanović, Dragana
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5387
AB  - Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.
PB  - Charles University, Czech Republic
T2  - Folia biologica
T1  - Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis
VL  - 69
IS  - 2
SP  - 59
EP  - 68
DO  - 10.14712/fb2023069020059
ER  - 

@article{
author = "Belić, Milica and Sopić, Miron and Roksandić-Milenković, Marina and Ćeriman, Vesna and Guzonjić, Azra and Vukašinović, Aleksandra and Ostanek, Barbara and Dimić, Nemanja and Jovanović, Dragana and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.",
publisher = "Charles University, Czech Republic",
journal = "Folia biologica",
title = "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis",
volume = "69",
number = "2",
pages = "59-68",
doi = "10.14712/fb2023069020059"
}

Belić, M., Sopić, M., Roksandić-Milenković, M., Ćeriman, V., Guzonjić, A., Vukašinović, A., Ostanek, B., Dimić, N., Jovanović, D.,& Kotur-Stevuljević, J.. (2023). Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica
Charles University, Czech Republic., 69(2), 59-68.
https://doi.org/10.14712/fb2023069020059

Belić M, Sopić M, Roksandić-Milenković M, Ćeriman V, Guzonjić A, Vukašinović A, Ostanek B, Dimić N, Jovanović D, Kotur-Stevuljević J. Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica. 2023;69(2):59-68.
doi:10.14712/fb2023069020059 .

Belić, Milica, Sopić, Miron, Roksandić-Milenković, Marina, Ćeriman, Vesna, Guzonjić, Azra, Vukašinović, Aleksandra, Ostanek, Barbara, Dimić, Nemanja, Jovanović, Dragana, Kotur-Stevuljević, Jelena, "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis" in Folia biologica, 69, no. 2 (2023):59-68,
https://doi.org/10.14712/fb2023069020059 . .

TY  - CONF
AU  - Guzonjić, Azra
AU  - Roksandić Milenković, Marina
AU  - Dimić, Dejan
AU  - Dimić, Nemanja
AU  - Timotijević, Ljiljana
AU  - Sopić, Miron
AU  - Vekić, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4510
AB  - The pathogenesis of the COVID-19 infection caused by the SARS-CoV-2 coronavirus is
the subject of active research worldwide. As a complex disease, it is suspected that oxidative
stress plays a role in its pathogenesis and contributes to the progressive shortening of
telomeres. Patients aged 25–84 years (n=31) were monitored at the time of diagnosis, as
well as at control on the 14th and 21st days. Redox status parameters were determined: total
oxidant status (TOS), superoxide anion radical (O2.-), prooxidant-antioxidant balance (PAB),
advanced protein oxidation products (AOPP), malondialdehyde (MDA), and ischemia-
modified albumin (IMA) as prooxidants and total antioxidant status (TAS), superoxide-
dismutase (SOD) and paraoxonase 1 (PON1) enzyme activity, total antioxidant and total
oxidant status ratio (TAS/TOS), and total sulfhydryl groups (SHG) as antioxidants. Patients
have the shortest telomeres at diagnosis and their length increases during recovery. A
statistically significant difference in rTL was shown in patients at the time of diagnosis and
after 21 days (p=0.008). rTL has a statistically significant negative correlation with SHG in
patients at the time of diagnosis (ρ=-0.386, p=0.038), as well as in patients after 14 days (ρ =-
0.389, p=0.037). After 21 days, a statistically significant positive correlation was shown
between rTL and SOD (ρ =0.381, p=0.046). The obtained results indicate progressive
shortening of telomeres caused by COVID-19 infection, impaired redox status, as well as the
change of these parameters during recovery, which confirms the link between oxidative
stress and rTL, as well as the participation of these processes in the pathogenesis of COVID-
19.
AB  - Patogeneza COVID-19 infekcije izazvane koronavirusom SARS-CoV-2 je predmet
aktivnog istraživanja širom sveta. Kako je u pitanju složena bolest, smatra se da i u njenoj
patogenezi leži oksidativni stres, koji doprinosi progesivnom skraćivanju telomera. Pacijenti
starosti 25-84 godina (n=31) praćeni su u trenutku postavljanja dijagnoze, kao i na kontroli
14. i 21. dana. Parametri redoks statusa određivani su iz seruma ili plazme odgovarajućim
spektrofotometrijskim metodama, dok je iz leukocita periferne krvi merena relativna dužina
telomera (rTL) pomoću qPCR metode. Od parametara oksidativnog statusa određivani su:
totalni oksidantni status (TOS), superoksidni anjon radikal (O2.- ), prooksidantno-
antioksidantni balans (PAB), uznapredovali produkti oksidacije proteina (AOPP),
malondialdehid (MDA), ishemijom modifikovan albumin (IMA). Kao parametri
antioksidativne zaštite određivani su: totalni antioksidantni status (TAS), aktivnost enzima
superoksid-dismutaze (SOD) i paraoksonaze 1 (PON1), odnos totalnog antioksidantnog i
totalnog oksidantnog statusa (TAS/TOS), kao i ukupne sulfhidrilne grupe (SHG). Pacijenti u
trenutku postavljanja dijagnoze imaju najkraće telomere i njihova dužina se povećava tokom
oporavka. Pokazana je statistički značajna razlika u rTL kod pacijenata u trenutku
postavljanja dijagnoze i nakon 21 dan (p=0,008). rTL je u statistički značajnoj negativnoj
korelaciji sa SHG kod pacijenata u trenutku postavljanja dijagnoze (ρ=-0,386, p=0,038), kao i
kod pacijenata nakon 14 dana oporavka (ρ=-0,389, p=0,037). Nakon 21 dan, pokazana je
statistički značajna pozitivna korelacija između rTL i SOD (ρ=0,381, p=0,046). Dobijeni
rezultati ukazuju na progresivno skraćenje telomera izazvano COVID-19 infekcijom, narušen
redoks status, kao i na promenu ovih parametara tokom oporavka, koja potvrđuje vezu
između oksidativnog stresa i rTL, kao i učešće ovih procesa u patogenezi COVID-19.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Oxidative stress and telomere length in COVID-19 patients
T1  - Oksidativni stres i dužina telomera kod COVID‐19 pacijenata
VL  - 72
IS  - 4 suplement
SP  - S231
EP  - S232
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4510
ER  - 

@conference{
author = "Guzonjić, Azra and Roksandić Milenković, Marina and Dimić, Dejan and Dimić, Nemanja and Timotijević, Ljiljana and Sopić, Miron and Vekić, Jelena and Bogavac-Stanojević, Nataša and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "The pathogenesis of the COVID-19 infection caused by the SARS-CoV-2 coronavirus is
the subject of active research worldwide. As a complex disease, it is suspected that oxidative
stress plays a role in its pathogenesis and contributes to the progressive shortening of
telomeres. Patients aged 25–84 years (n=31) were monitored at the time of diagnosis, as
well as at control on the 14th and 21st days. Redox status parameters were determined: total
oxidant status (TOS), superoxide anion radical (O2.-), prooxidant-antioxidant balance (PAB),
advanced protein oxidation products (AOPP), malondialdehyde (MDA), and ischemia-
modified albumin (IMA) as prooxidants and total antioxidant status (TAS), superoxide-
dismutase (SOD) and paraoxonase 1 (PON1) enzyme activity, total antioxidant and total
oxidant status ratio (TAS/TOS), and total sulfhydryl groups (SHG) as antioxidants. Patients
have the shortest telomeres at diagnosis and their length increases during recovery. A
statistically significant difference in rTL was shown in patients at the time of diagnosis and
after 21 days (p=0.008). rTL has a statistically significant negative correlation with SHG in
patients at the time of diagnosis (ρ=-0.386, p=0.038), as well as in patients after 14 days (ρ =-
0.389, p=0.037). After 21 days, a statistically significant positive correlation was shown
between rTL and SOD (ρ =0.381, p=0.046). The obtained results indicate progressive
shortening of telomeres caused by COVID-19 infection, impaired redox status, as well as the
change of these parameters during recovery, which confirms the link between oxidative
stress and rTL, as well as the participation of these processes in the pathogenesis of COVID-
19., Patogeneza COVID-19 infekcije izazvane koronavirusom SARS-CoV-2 je predmet
aktivnog istraživanja širom sveta. Kako je u pitanju složena bolest, smatra se da i u njenoj
patogenezi leži oksidativni stres, koji doprinosi progesivnom skraćivanju telomera. Pacijenti
starosti 25-84 godina (n=31) praćeni su u trenutku postavljanja dijagnoze, kao i na kontroli
14. i 21. dana. Parametri redoks statusa određivani su iz seruma ili plazme odgovarajućim
spektrofotometrijskim metodama, dok je iz leukocita periferne krvi merena relativna dužina
telomera (rTL) pomoću qPCR metode. Od parametara oksidativnog statusa određivani su:
totalni oksidantni status (TOS), superoksidni anjon radikal (O2.- ), prooksidantno-
antioksidantni balans (PAB), uznapredovali produkti oksidacije proteina (AOPP),
malondialdehid (MDA), ishemijom modifikovan albumin (IMA). Kao parametri
antioksidativne zaštite određivani su: totalni antioksidantni status (TAS), aktivnost enzima
superoksid-dismutaze (SOD) i paraoksonaze 1 (PON1), odnos totalnog antioksidantnog i
totalnog oksidantnog statusa (TAS/TOS), kao i ukupne sulfhidrilne grupe (SHG). Pacijenti u
trenutku postavljanja dijagnoze imaju najkraće telomere i njihova dužina se povećava tokom
oporavka. Pokazana je statistički značajna razlika u rTL kod pacijenata u trenutku
postavljanja dijagnoze i nakon 21 dan (p=0,008). rTL je u statistički značajnoj negativnoj
korelaciji sa SHG kod pacijenata u trenutku postavljanja dijagnoze (ρ=-0,386, p=0,038), kao i
kod pacijenata nakon 14 dana oporavka (ρ=-0,389, p=0,037). Nakon 21 dan, pokazana je
statistički značajna pozitivna korelacija između rTL i SOD (ρ=0,381, p=0,046). Dobijeni
rezultati ukazuju na progresivno skraćenje telomera izazvano COVID-19 infekcijom, narušen
redoks status, kao i na promenu ovih parametara tokom oporavka, koja potvrđuje vezu
između oksidativnog stresa i rTL, kao i učešće ovih procesa u patogenezi COVID-19.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Oxidative stress and telomere length in COVID-19 patients, Oksidativni stres i dužina telomera kod COVID‐19 pacijenata",
volume = "72",
number = "4 suplement",
pages = "S231-S232",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4510"
}

Guzonjić, A., Roksandić Milenković, M., Dimić, D., Dimić, N., Timotijević, L., Sopić, M., Vekić, J., Bogavac-Stanojević, N.,& Kotur-Stevuljević, J.. (2022). Oxidative stress and telomere length in COVID-19 patients. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S231-S232.
https://hdl.handle.net/21.15107/rcub_farfar_4510

Guzonjić A, Roksandić Milenković M, Dimić D, Dimić N, Timotijević L, Sopić M, Vekić J, Bogavac-Stanojević N, Kotur-Stevuljević J. Oxidative stress and telomere length in COVID-19 patients. in Arhiv za farmaciju. 2022;72(4 suplement):S231-S232.
https://hdl.handle.net/21.15107/rcub_farfar_4510 .

Guzonjić, Azra, Roksandić Milenković, Marina, Dimić, Dejan, Dimić, Nemanja, Timotijević, Ljiljana, Sopić, Miron, Vekić, Jelena, Bogavac-Stanojević, Nataša, Kotur-Stevuljević, Jelena, "Oxidative stress and telomere length in COVID-19 patients" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S231-S232,
https://hdl.handle.net/21.15107/rcub_farfar_4510 .

TY  - CONF
AU  - Erceg, Sanja
AU  - Tomašević, Ratko
AU  - Ninić, Ana
AU  - Pavlović, Aleksandar
AU  - Guzonjić, Azra
AU  - Vujčić, Sanja
AU  - Mamić, Milica
AU  - Mitrović, Bojan
AU  - Gluvić, Zoran
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4511
AB  - Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease,
present in up to 30% of the adult population worldwide (1). Triglycerides accumulation in
hepatocytes (steatosis) represents the root cause of NAFLD and is associated with oxidative
stress, which could further lead to fibrosis and cell death of hepatocytes (2). The aim of this
research was to identify redox status markers for predicting the risk of developing steatosis.
158 participants were included. Steatosis was confirmed by ultrasound in 101 subjects,
while the remaining 57 were in the control group. The following markers of redox status
were determined in serum and plasma samples of all subjects: superoxide dismutase 1
(SOD1), paraoxonase (PON1), malondialdehyde (MDA) and superoxide anion (O2.-). For this
purpose, spectrophotometric methods and enzyme immunosorbent assays were used. SOD1
was statistically significantly higher (P<0.001), while O2 .- was significantly lower in the
patient group (P<0.001). SOD1 was significantly negatively correlated with O2.- (ρ= -0.494,
P<0.001) and MDA (ρ= -0.242, P=0.002). Univariate binary logistic regression analysis
showed a positive association between SOD1 and the presence of steatosis (OR=1.018, 95%
CI 1.005-1.031; P=0.005), as well as a negative association between O2 .- and the presence of
steatosis (OR=0.959, 95% CI 0.941-0.978; P<0.001). Multivariate analysis singled out SOD1
(OR=1.024, 95% CI 1.006-1.041; P=0.007) and O2.- (OR=0.965, 95% CI 0.942-0.989;
P=0.004) as independent predictors for the presence of steatosis in our subjects. The redox
status parameters, SOD1 and O2, respectively, showed a positive and negative prediction of
the presence of statosis in our subjects.
AB  - Nealkoholna masna bolest jetre (eng. nonalcoholic fatty liver disease, NAFLD) je
najčešće hronično oboljenje jetre, prisutno i u do 30% adultne populacije širom sveta (1).
Značajnu ulogu u nastanku NAFLD ima akumulacija triglicerida u hepatocitima – steatoza,
koja je povezana sa oksidativnim stresom, a koji dalje vodi fibrozi i ćelijskoj smrti hepatocita
(2). Cilj ovog istraživanja bio je identifikacija markera redoks statusa za predviđanje rizika za
nastanak steatoze. Studija je obuhvatila 158 ispitanika iz Kliničko bolničkog centra Zemun.
Steatoza je potvrđena ultrazvukom kod 101 ispitanika, dok je preostalih 57 činilo kontrolnu
grupu. U uzorcima seruma i plazme svih ispitanika određeni su sledeći markeri redoks
statusa: superoksid dismutaza 1 (SOD1), paraoksonaza (PON1), malondialdehid (MDA) i
superoksidni anjon (O2.-). U tu svrhu korišćene su spektrofotometrijske metode i enzimski
imunosorbentni testovi. SOD1 je bila statistički značajno viša (P<0,001), dok O 2.- značajno
niži u grupi pacijenata (P<0,001), dok se PON1 i MDA nisu značajno razlikovali između
grupa. SOD1 je bila u značajnoj negativnoj korelaciji sa O 2.- (ρ=-0,494, P<0,001) i MDA (ρ= -
0,242, P=0,002). Univarijatna binarna logistička regresiona analiza je pokazala pozitivnu
asocijaciju između SOD1 i prisustva steatoze (OR=1,018, 95% CI 1,005-1,031; P=0,005), kao i
negativnu asocijaciju između O 2.- i prisustva steatoze (OR=0,959, 95% CI 0,941-0,978;
P<0,001). Multivarijantna analiza je izdvojila SOD1 (OR=1,024, 95% CI 1,006-1,041;
P=0,007) i O2.- (OR=0,965, 95% CI 0,942-0,989; P=0,004) kao nezavisne prediktore za
prisustvo steatoze kod naših ispitanika. Parametri redoks statusa, SOD1 i O 2.- redom, su
pokazali pozitivnu, odnosno negativnu predikciju prisustva statoze kod naših ispitanika.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Markers of redox status in patients with nonalcoholic fatty liver disease
T1  - Markeri redoks statusa kod pacijenata sa nealkoholnom masnom bolešću jetre
VL  - 72
IS  - 4 suplement
SP  - S233
EP  - S234
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4511
ER  - 

@conference{
author = "Erceg, Sanja and Tomašević, Ratko and Ninić, Ana and Pavlović, Aleksandar and Guzonjić, Azra and Vujčić, Sanja and Mamić, Milica and Mitrović, Bojan and Gluvić, Zoran and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease,
present in up to 30% of the adult population worldwide (1). Triglycerides accumulation in
hepatocytes (steatosis) represents the root cause of NAFLD and is associated with oxidative
stress, which could further lead to fibrosis and cell death of hepatocytes (2). The aim of this
research was to identify redox status markers for predicting the risk of developing steatosis.
158 participants were included. Steatosis was confirmed by ultrasound in 101 subjects,
while the remaining 57 were in the control group. The following markers of redox status
were determined in serum and plasma samples of all subjects: superoxide dismutase 1
(SOD1), paraoxonase (PON1), malondialdehyde (MDA) and superoxide anion (O2.-). For this
purpose, spectrophotometric methods and enzyme immunosorbent assays were used. SOD1
was statistically significantly higher (P<0.001), while O2 .- was significantly lower in the
patient group (P<0.001). SOD1 was significantly negatively correlated with O2.- (ρ= -0.494,
P<0.001) and MDA (ρ= -0.242, P=0.002). Univariate binary logistic regression analysis
showed a positive association between SOD1 and the presence of steatosis (OR=1.018, 95%
CI 1.005-1.031; P=0.005), as well as a negative association between O2 .- and the presence of
steatosis (OR=0.959, 95% CI 0.941-0.978; P<0.001). Multivariate analysis singled out SOD1
(OR=1.024, 95% CI 1.006-1.041; P=0.007) and O2.- (OR=0.965, 95% CI 0.942-0.989;
P=0.004) as independent predictors for the presence of steatosis in our subjects. The redox
status parameters, SOD1 and O2, respectively, showed a positive and negative prediction of
the presence of statosis in our subjects., Nealkoholna masna bolest jetre (eng. nonalcoholic fatty liver disease, NAFLD) je
najčešće hronično oboljenje jetre, prisutno i u do 30% adultne populacije širom sveta (1).
Značajnu ulogu u nastanku NAFLD ima akumulacija triglicerida u hepatocitima – steatoza,
koja je povezana sa oksidativnim stresom, a koji dalje vodi fibrozi i ćelijskoj smrti hepatocita
(2). Cilj ovog istraživanja bio je identifikacija markera redoks statusa za predviđanje rizika za
nastanak steatoze. Studija je obuhvatila 158 ispitanika iz Kliničko bolničkog centra Zemun.
Steatoza je potvrđena ultrazvukom kod 101 ispitanika, dok je preostalih 57 činilo kontrolnu
grupu. U uzorcima seruma i plazme svih ispitanika određeni su sledeći markeri redoks
statusa: superoksid dismutaza 1 (SOD1), paraoksonaza (PON1), malondialdehid (MDA) i
superoksidni anjon (O2.-). U tu svrhu korišćene su spektrofotometrijske metode i enzimski
imunosorbentni testovi. SOD1 je bila statistički značajno viša (P<0,001), dok O 2.- značajno
niži u grupi pacijenata (P<0,001), dok se PON1 i MDA nisu značajno razlikovali između
grupa. SOD1 je bila u značajnoj negativnoj korelaciji sa O 2.- (ρ=-0,494, P<0,001) i MDA (ρ= -
0,242, P=0,002). Univarijatna binarna logistička regresiona analiza je pokazala pozitivnu
asocijaciju između SOD1 i prisustva steatoze (OR=1,018, 95% CI 1,005-1,031; P=0,005), kao i
negativnu asocijaciju između O 2.- i prisustva steatoze (OR=0,959, 95% CI 0,941-0,978;
P<0,001). Multivarijantna analiza je izdvojila SOD1 (OR=1,024, 95% CI 1,006-1,041;
P=0,007) i O2.- (OR=0,965, 95% CI 0,942-0,989; P=0,004) kao nezavisne prediktore za
prisustvo steatoze kod naših ispitanika. Parametri redoks statusa, SOD1 i O 2.- redom, su
pokazali pozitivnu, odnosno negativnu predikciju prisustva statoze kod naših ispitanika.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Markers of redox status in patients with nonalcoholic fatty liver disease, Markeri redoks statusa kod pacijenata sa nealkoholnom masnom bolešću jetre",
volume = "72",
number = "4 suplement",
pages = "S233-S234",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4511"
}

Erceg, S., Tomašević, R., Ninić, A., Pavlović, A., Guzonjić, A., Vujčić, S., Mamić, M., Mitrović, B., Gluvić, Z.,& Kotur-Stevuljević, J.. (2022). Markers of redox status in patients with nonalcoholic fatty liver disease. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S233-S234.
https://hdl.handle.net/21.15107/rcub_farfar_4511

Erceg S, Tomašević R, Ninić A, Pavlović A, Guzonjić A, Vujčić S, Mamić M, Mitrović B, Gluvić Z, Kotur-Stevuljević J. Markers of redox status in patients with nonalcoholic fatty liver disease. in Arhiv za farmaciju. 2022;72(4 suplement):S233-S234.
https://hdl.handle.net/21.15107/rcub_farfar_4511 .

Erceg, Sanja, Tomašević, Ratko, Ninić, Ana, Pavlović, Aleksandar, Guzonjić, Azra, Vujčić, Sanja, Mamić, Milica, Mitrović, Bojan, Gluvić, Zoran, Kotur-Stevuljević, Jelena, "Markers of redox status in patients with nonalcoholic fatty liver disease" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S233-S234,
https://hdl.handle.net/21.15107/rcub_farfar_4511 .

TY  - CONF
AU  - Crevar, Milkica
AU  - Guzonjić, Azra
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4444
AB  - Oxidative stress is a phenomenon that occurs due to the disturbance in the balance
between the production of reactive oxygen species and the ability of biological systems to
remove the resulting compounds. Oxidative stress is involved in the pathogenesis of many
disorders in the human organism. This indicates the importance of quantification of
oxidative stress parameters in biological samples. Traditionally, these parameters are
determined by biochemical tests. Although these tests are routinely performed, they have
many drawbacks. To determine the exact concentration of selected compounds, more
sensitive analytical methods are becoming more important. In the modern scientific
literature, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly
mentioned (1). This method, with adequate selection of stationary and mobile phases,
enables quantification of very low concentrations of selected parameters. In addition, these
methods can simultaneously determine the concentration of many selected components.
However, it is necessary to take into account that LC-MS/MS methods require a very purified
biological sample from which the proteins have been maximally removed. In this work, we
will present the results of determination of cysteine, cystine, reduced and oxidized
glutathione in patients with microcellular lung cancer. The use of LC-MS/MS methods is
becoming increasingly common for the analysis of oxidative stress markers in biological
fluids. In the future, we are expected to move to even more modern, fully automated
methods, which simultaneously purify and analyze samples.
AB  - Oksidativni stres je pojava koja nastaje usled narušavanja ravnoteže između
proizvodnje i nagomilavanja reaktivnih jedinjenja kiseonika u organizmu i sposobnosti
bioloških sistema da ukloni nastala jedinjenja. Oksidativni stres učestvuje u patogenezi
mnogih poremećaja kao što su kardiovaskularne bolesti, dijabetes i bolesti bubrega. Ovo
ukazuje na značaj određivanja odabranih parametara oksidativnog stresa u uzorcima
biološkog materijala. Tradicionalno, najveći broj parametara se određuje biohemijskim
testovima. Iako se ovi testovi rutinski izvode, oni imaju mnoge nedostatke. Da bi se odredila
tačna koncentracija izabranih parametara, koji se u uzorcima nalaze u veoma niskim
koncentracijama, sve veću prednost imaju osetljivije analitičke metode. U savremenoj
naučnoj literaturi se u te svrhe sve više spominje tečna hromatografija spregnuta sa
masenim detektorom (LC-MS/MS) (1). Ova metoda, uz adekvatan izbor stacionarne i
mobilne faze, omogućava kvantifikaciju veoma niskih koncentracija odabranih jedinjenja.
Sem toga, ovim metodama se istovremeno, u jednom uzorku, može odrediti koncentracija
većeg broja odabranih komponenti. Ipak, neophodno je voditi računa o tome da tečna
hromatografija spregnuta sa masenim detektorom zahteva dobro prečišćen uzorak biološkog
materijala iz kog su maksimalno uklonjeni proteini. U ovom radu biće prikazani rezultati
određivanja cisteina, cistina, redukovanog i oksidovanog glutationa kod pacijenata sa
mikrocelularnim karcinomom pluća. Upotreba LC-MS/MS metoda je sve uobičajenija za
analizu markera oksidativnog stresa u biološkim tečnostima zbog svoje osetljivosti. U
budućnosti se očekuje razvoj ka još savremenijim, potpuno automatizovanim metodama,
kojima se istovremeno uzorci prečišćavaju i analiziraju.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Application of LC-MS/MS methods in the quantification of oxidative stress parameters
T1  - Primena LC‐MS/MS metoda u ispitivanju parametara oksidativnog stresa
VL  - 72
IS  - 4 suplement
SP  - S51
EP  - S52
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4444
ER  - 

@conference{
author = "Crevar, Milkica and Guzonjić, Azra and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "Oxidative stress is a phenomenon that occurs due to the disturbance in the balance
between the production of reactive oxygen species and the ability of biological systems to
remove the resulting compounds. Oxidative stress is involved in the pathogenesis of many
disorders in the human organism. This indicates the importance of quantification of
oxidative stress parameters in biological samples. Traditionally, these parameters are
determined by biochemical tests. Although these tests are routinely performed, they have
many drawbacks. To determine the exact concentration of selected compounds, more
sensitive analytical methods are becoming more important. In the modern scientific
literature, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly
mentioned (1). This method, with adequate selection of stationary and mobile phases,
enables quantification of very low concentrations of selected parameters. In addition, these
methods can simultaneously determine the concentration of many selected components.
However, it is necessary to take into account that LC-MS/MS methods require a very purified
biological sample from which the proteins have been maximally removed. In this work, we
will present the results of determination of cysteine, cystine, reduced and oxidized
glutathione in patients with microcellular lung cancer. The use of LC-MS/MS methods is
becoming increasingly common for the analysis of oxidative stress markers in biological
fluids. In the future, we are expected to move to even more modern, fully automated
methods, which simultaneously purify and analyze samples., Oksidativni stres je pojava koja nastaje usled narušavanja ravnoteže između
proizvodnje i nagomilavanja reaktivnih jedinjenja kiseonika u organizmu i sposobnosti
bioloških sistema da ukloni nastala jedinjenja. Oksidativni stres učestvuje u patogenezi
mnogih poremećaja kao što su kardiovaskularne bolesti, dijabetes i bolesti bubrega. Ovo
ukazuje na značaj određivanja odabranih parametara oksidativnog stresa u uzorcima
biološkog materijala. Tradicionalno, najveći broj parametara se određuje biohemijskim
testovima. Iako se ovi testovi rutinski izvode, oni imaju mnoge nedostatke. Da bi se odredila
tačna koncentracija izabranih parametara, koji se u uzorcima nalaze u veoma niskim
koncentracijama, sve veću prednost imaju osetljivije analitičke metode. U savremenoj
naučnoj literaturi se u te svrhe sve više spominje tečna hromatografija spregnuta sa
masenim detektorom (LC-MS/MS) (1). Ova metoda, uz adekvatan izbor stacionarne i
mobilne faze, omogućava kvantifikaciju veoma niskih koncentracija odabranih jedinjenja.
Sem toga, ovim metodama se istovremeno, u jednom uzorku, može odrediti koncentracija
većeg broja odabranih komponenti. Ipak, neophodno je voditi računa o tome da tečna
hromatografija spregnuta sa masenim detektorom zahteva dobro prečišćen uzorak biološkog
materijala iz kog su maksimalno uklonjeni proteini. U ovom radu biće prikazani rezultati
određivanja cisteina, cistina, redukovanog i oksidovanog glutationa kod pacijenata sa
mikrocelularnim karcinomom pluća. Upotreba LC-MS/MS metoda je sve uobičajenija za
analizu markera oksidativnog stresa u biološkim tečnostima zbog svoje osetljivosti. U
budućnosti se očekuje razvoj ka još savremenijim, potpuno automatizovanim metodama,
kojima se istovremeno uzorci prečišćavaju i analiziraju.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Application of LC-MS/MS methods in the quantification of oxidative stress parameters, Primena LC‐MS/MS metoda u ispitivanju parametara oksidativnog stresa",
volume = "72",
number = "4 suplement",
pages = "S51-S52",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4444"
}

Crevar, M., Guzonjić, A.,& Kotur-Stevuljević, J.. (2022). Application of LC-MS/MS methods in the quantification of oxidative stress parameters. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S51-S52.
https://hdl.handle.net/21.15107/rcub_farfar_4444

Crevar M, Guzonjić A, Kotur-Stevuljević J. Application of LC-MS/MS methods in the quantification of oxidative stress parameters. in Arhiv za farmaciju. 2022;72(4 suplement):S51-S52.
https://hdl.handle.net/21.15107/rcub_farfar_4444 .

Crevar, Milkica, Guzonjić, Azra, Kotur-Stevuljević, Jelena, "Application of LC-MS/MS methods in the quantification of oxidative stress parameters" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S51-S52,
https://hdl.handle.net/21.15107/rcub_farfar_4444 .

TY  - JOUR
AU  - Vujčić, Sanja
AU  - Kotur-Stevuljević, Jelena
AU  - Vekić, Jelena
AU  - Perović-Blagojević, Iva
AU  - Stefanović, Tatjana
AU  - Ilić-Mijailović, Sanja
AU  - Koprivica Uzelac, Branka
AU  - Bosić, Srećko
AU  - Antonić, Tamara
AU  - Guzonjić, Azra
AU  - Mastilović, Ana-Marija
AU  - Marković, Zorica
AU  - Rizzo, Manfredi
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4369
AB  - Background and Objectives: Diabetic foot (DF) development is driven by complex interactions of hyperglycemia, inflammation, and oxidative stress (OS). We aimed to investigate OS and inflammatory biomarkers in patients with DF and their potential to improve early diagnosis and management of DF. Materials and Methods: The prooxidant–antioxidant balance (PAB), superoxide dismutase (SOD), total oxidative status (TOS), total sulfhydryl groups (SHG), routine biochemical parameters, and complete blood count were determined in 42 patients with type-2 DM, of which 23 patients had DF, while 19 patients were without DF complications. The neutrophils-to-lymphocyte ratio (NLR) was evaluated as a biomarker of inflammation. Results: Patients with DF had significantly higher (p < 0.05) PAB levels (170 ± 33.9 U/L) compared to those without DF complications (142 ± 31.3 U/L). In addition, patients with DF had significantly reduced SOD activities (p < 0.01). NLR values were significantly higher in the DF group (median: 2.8; interquartile range: 2.0–4.3) than in the group without DF (median: 1.4; interquartile range: 1.4–2.1; p < 0.01). A positive correlation was found between the PAB and NLR index (r = 0.449; p < 0.05). The diagnostic accuracy of both PAB (AUC = 0.741; p < 0.01) and NLR (AUC = 0.760; p < 0.01) was estimated as acceptable. Conclusions: In conclusion, the development of DF is associated with enhanced OS and inflammation processes. PAB and NLR could be useful non-invasive biomarkers of DF development.
PB  - MDPI
T2  - Medicina (Lithuania)
T1  - Oxidative Stress and Inflammatory Biomarkers in Patients with Diabetic Foot
VL  - 58
IS  - 12
DO  - 10.3390/medicina58121866
ER  - 

@article{
author = "Vujčić, Sanja and Kotur-Stevuljević, Jelena and Vekić, Jelena and Perović-Blagojević, Iva and Stefanović, Tatjana and Ilić-Mijailović, Sanja and Koprivica Uzelac, Branka and Bosić, Srećko and Antonić, Tamara and Guzonjić, Azra and Mastilović, Ana-Marija and Marković, Zorica and Rizzo, Manfredi",
year = "2022",
abstract = "Background and Objectives: Diabetic foot (DF) development is driven by complex interactions of hyperglycemia, inflammation, and oxidative stress (OS). We aimed to investigate OS and inflammatory biomarkers in patients with DF and their potential to improve early diagnosis and management of DF. Materials and Methods: The prooxidant–antioxidant balance (PAB), superoxide dismutase (SOD), total oxidative status (TOS), total sulfhydryl groups (SHG), routine biochemical parameters, and complete blood count were determined in 42 patients with type-2 DM, of which 23 patients had DF, while 19 patients were without DF complications. The neutrophils-to-lymphocyte ratio (NLR) was evaluated as a biomarker of inflammation. Results: Patients with DF had significantly higher (p < 0.05) PAB levels (170 ± 33.9 U/L) compared to those without DF complications (142 ± 31.3 U/L). In addition, patients with DF had significantly reduced SOD activities (p < 0.01). NLR values were significantly higher in the DF group (median: 2.8; interquartile range: 2.0–4.3) than in the group without DF (median: 1.4; interquartile range: 1.4–2.1; p < 0.01). A positive correlation was found between the PAB and NLR index (r = 0.449; p < 0.05). The diagnostic accuracy of both PAB (AUC = 0.741; p < 0.01) and NLR (AUC = 0.760; p < 0.01) was estimated as acceptable. Conclusions: In conclusion, the development of DF is associated with enhanced OS and inflammation processes. PAB and NLR could be useful non-invasive biomarkers of DF development.",
publisher = "MDPI",
journal = "Medicina (Lithuania)",
title = "Oxidative Stress and Inflammatory Biomarkers in Patients with Diabetic Foot",
volume = "58",
number = "12",
doi = "10.3390/medicina58121866"
}

Vujčić, S., Kotur-Stevuljević, J., Vekić, J., Perović-Blagojević, I., Stefanović, T., Ilić-Mijailović, S., Koprivica Uzelac, B., Bosić, S., Antonić, T., Guzonjić, A., Mastilović, A., Marković, Z.,& Rizzo, M.. (2022). Oxidative Stress and Inflammatory Biomarkers in Patients with Diabetic Foot. in Medicina (Lithuania)
MDPI., 58(12).
https://doi.org/10.3390/medicina58121866

Vujčić S, Kotur-Stevuljević J, Vekić J, Perović-Blagojević I, Stefanović T, Ilić-Mijailović S, Koprivica Uzelac B, Bosić S, Antonić T, Guzonjić A, Mastilović A, Marković Z, Rizzo M. Oxidative Stress and Inflammatory Biomarkers in Patients with Diabetic Foot. in Medicina (Lithuania). 2022;58(12).
doi:10.3390/medicina58121866 .

Vujčić, Sanja, Kotur-Stevuljević, Jelena, Vekić, Jelena, Perović-Blagojević, Iva, Stefanović, Tatjana, Ilić-Mijailović, Sanja, Koprivica Uzelac, Branka, Bosić, Srećko, Antonić, Tamara, Guzonjić, Azra, Mastilović, Ana-Marija, Marković, Zorica, Rizzo, Manfredi, "Oxidative Stress and Inflammatory Biomarkers in Patients with Diabetic Foot" in Medicina (Lithuania), 58, no. 12 (2022),
https://doi.org/10.3390/medicina58121866 . .

TY  - JOUR
AU  - Guzonjić, Azra
AU  - Sopić, Miron
AU  - Ostanek, Barbara
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4185
AB  - As research related to healthspan and lifespan has become a hot topic, the necessity for a
reliable and practical biomarker of aging (BoA), which can provide information about mortality
and morbidity risk, along with remaining life expectancy, has increased. The chromosome
terminus non-coding protective structure that prevents genomic instability is called a telomere.
The continual shortening of telomeres, which affects their structure as well as function, is a
hallmark of agedness. The aforementioned process is a potential cause of age-related diseases
(ARDs), leading to a bad prognosis and a low survival rate, which compromise health and
longevity. Hence, studies scrutinizing the BoAs often include telomere length (TL) as a
prospective candidate. The results of these studies suggest that TL measurement can only provide
an approximate appraisal of the aging rate, and its implementation into clinical practice and
routine use as a BoA has many limitations and challenges. Nevertheless, measuring TL while
determining other biomarkers can be used to assess biological age. This review focuses on the
importance of telomeres in health, senescence, and diseases, as well as on summarizing the results
and conclusions of previous studies evaluating TL as a potential BoA.
AB  - Kako su istraživanja na temu starenja postala sve popularnija, javila se potreba za pouzdanim i praktičnim biomarkerom starenja, koji može pružiti informacije o riziku od mortaliteta i morbiditeta. Telomere su nekodirajući krajevi linearnih hromozoma koji održavaju njihovu stabilnost i integritet. Ćelijsko starenje i starenje organizma karakteriše progresivno skraćivanje telomera, što ugrožava njihovu strukturu i funkciju. Skraćivanje telomera je u vezi sa povećanom incidencom bolesti povezanih sa starenjem i lošom stopom preživljavanja, što ugrožava zdravlje i skraćuje životni vek. Stoga je dužina telomera dugo vremena prepoznata kao jedan od najboljih biomarkera starenja. Međutim, nedavna istraživanja ukazuju na to da dužina telomera može da pruži samo približnu procenu brzine starenja, pa je implementacija ovog biomarkera u kliničku praksu i rutinsku primenu praćena mnogim ograničenjima i izazovima. Uprkos tome, merenje dužine telomera, uz istovremeno određivanje drugih biomarkera, može poslužiti za procenu biološke starosti. Fokus ovog rada je na značaju telomera u ljudskom zdravlju, starenju i bolestima, kao i na sumiranju rezultata i zaključaka dosadašnjih studija koje su se bavile ispitivanjem dužine telomera kao potencijalnog kandidata za biomarker starenja.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Telomere length as a biomarker of aging and diseases
T1  - Dužina telomera kao biomarker starenja i bolesti
VL  - 72
IS  - 2
SP  - 105
EP  - 126
DO  - 10.5937/arhfarm72-36376
ER  - 

@article{
author = "Guzonjić, Azra and Sopić, Miron and Ostanek, Barbara and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "As research related to healthspan and lifespan has become a hot topic, the necessity for a
reliable and practical biomarker of aging (BoA), which can provide information about mortality
and morbidity risk, along with remaining life expectancy, has increased. The chromosome
terminus non-coding protective structure that prevents genomic instability is called a telomere.
The continual shortening of telomeres, which affects their structure as well as function, is a
hallmark of agedness. The aforementioned process is a potential cause of age-related diseases
(ARDs), leading to a bad prognosis and a low survival rate, which compromise health and
longevity. Hence, studies scrutinizing the BoAs often include telomere length (TL) as a
prospective candidate. The results of these studies suggest that TL measurement can only provide
an approximate appraisal of the aging rate, and its implementation into clinical practice and
routine use as a BoA has many limitations and challenges. Nevertheless, measuring TL while
determining other biomarkers can be used to assess biological age. This review focuses on the
importance of telomeres in health, senescence, and diseases, as well as on summarizing the results
and conclusions of previous studies evaluating TL as a potential BoA., Kako su istraživanja na temu starenja postala sve popularnija, javila se potreba za pouzdanim i praktičnim biomarkerom starenja, koji može pružiti informacije o riziku od mortaliteta i morbiditeta. Telomere su nekodirajući krajevi linearnih hromozoma koji održavaju njihovu stabilnost i integritet. Ćelijsko starenje i starenje organizma karakteriše progresivno skraćivanje telomera, što ugrožava njihovu strukturu i funkciju. Skraćivanje telomera je u vezi sa povećanom incidencom bolesti povezanih sa starenjem i lošom stopom preživljavanja, što ugrožava zdravlje i skraćuje životni vek. Stoga je dužina telomera dugo vremena prepoznata kao jedan od najboljih biomarkera starenja. Međutim, nedavna istraživanja ukazuju na to da dužina telomera može da pruži samo približnu procenu brzine starenja, pa je implementacija ovog biomarkera u kliničku praksu i rutinsku primenu praćena mnogim ograničenjima i izazovima. Uprkos tome, merenje dužine telomera, uz istovremeno određivanje drugih biomarkera, može poslužiti za procenu biološke starosti. Fokus ovog rada je na značaju telomera u ljudskom zdravlju, starenju i bolestima, kao i na sumiranju rezultata i zaključaka dosadašnjih studija koje su se bavile ispitivanjem dužine telomera kao potencijalnog kandidata za biomarker starenja.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Telomere length as a biomarker of aging and diseases, Dužina telomera kao biomarker starenja i bolesti",
volume = "72",
number = "2",
pages = "105-126",
doi = "10.5937/arhfarm72-36376"
}

Guzonjić, A., Sopić, M., Ostanek, B.,& Kotur-Stevuljević, J.. (2022). Telomere length as a biomarker of aging and diseases. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 72(2), 105-126.
https://doi.org/10.5937/arhfarm72-36376

Guzonjić A, Sopić M, Ostanek B, Kotur-Stevuljević J. Telomere length as a biomarker of aging and diseases. in Arhiv za farmaciju. 2022;72(2):105-126.
doi:10.5937/arhfarm72-36376 .

Guzonjić, Azra, Sopić, Miron, Ostanek, Barbara, Kotur-Stevuljević, Jelena, "Telomere length as a biomarker of aging and diseases" in Arhiv za farmaciju, 72, no. 2 (2022):105-126,
https://doi.org/10.5937/arhfarm72-36376 . .