TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Micić, Svetlana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1734
AB  - A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations
VL  - 59
IS  - 2
SP  - 436
EP  - 441
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1734
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Micić, Svetlana",
year = "2012",
abstract = "A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations",
volume = "59",
number = "2",
pages = "436-441",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1734"
}

Kuntić, V., Pejić, N.,& Micić, S.. (2012). Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 59(2), 436-441.
https://hdl.handle.net/21.15107/rcub_farfar_1734

Kuntić V, Pejić N, Micić S. Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica. 2012;59(2):436-441.
https://hdl.handle.net/21.15107/rcub_farfar_1734 .

Kuntić, Vesna, Pejić, Nataša, Micić, Svetlana, "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations" in Acta Chimica Slovenica, 59, no. 2 (2012):436-441,
https://hdl.handle.net/21.15107/rcub_farfar_1734 .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Ivković, Branka
AU  - Vujić, Zorica
AU  - Ilić, Katarina
AU  - Micić, Svetlana
AU  - Vukojević, Vladana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/944
AB  - A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Isocratic RP-HPLC method for rutin determination in solid oral dosage forms
VL  - 43
IS  - 2
SP  - 718
EP  - 721
DO  - 10.1016/j.jpba.2006.07.019
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Ivković, Branka and Vujić, Zorica and Ilić, Katarina and Micić, Svetlana and Vukojević, Vladana",
year = "2007",
abstract = "A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms",
volume = "43",
number = "2",
pages = "718-721",
doi = "10.1016/j.jpba.2006.07.019"
}

Kuntić, V., Pejić, N., Ivković, B., Vujić, Z., Ilić, K., Micić, S.,& Vukojević, V.. (2007). Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 43(2), 718-721.
https://doi.org/10.1016/j.jpba.2006.07.019

Kuntić V, Pejić N, Ivković B, Vujić Z, Ilić K, Micić S, Vukojević V. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis. 2007;43(2):718-721.
doi:10.1016/j.jpba.2006.07.019 .

Kuntić, Vesna, Pejić, Nataša, Ivković, Branka, Vujić, Zorica, Ilić, Katarina, Micić, Svetlana, Vukojević, Vladana, "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms" in Journal of Pharmaceutical and Biomedical Analysis, 43, no. 2 (2007):718-721,
https://doi.org/10.1016/j.jpba.2006.07.019 . .