TY  - JOUR
AU  - Rauner, Martina
AU  - Foessl, Ines
AU  - Formosa, Melissa
AU  - Kague, Erika
AU  - Prijatelj, Vid
AU  - Alonso Lopez, Nerea
AU  - Banerjee, Bodhisattwa
AU  - Bergen, Dylan
AU  - Busse, Björn
AU  - Calado, Angelo
AU  - Douni, Eleni
AU  - Gabet, Yankel
AU  - Garcı´a Giralt, Natalia
AU  - Grinberg, Daniel
AU  - Lovsin, Nika
AU  - Nogues Solan, Xavier
AU  - Ostanek, Barbara
AU  - Pavlos, Nathan
AU  - Rivadeneira, Fernando
AU  - Soldatović, Ivan
AU  - van de Peppel, Jeroen
AU  - van der Eerden, Bram
AU  - van Hul, Wim
AU  - Balcells, Susanna
AU  - Marc, Janja
AU  - Reppe, Sjur
AU  - Søe, Kent
AU  - Karasik, David
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4019
AB  - The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques
VL  - 12
DO  - 10.3389/fendo.2021.731217
ER  - 

@article{
author = "Rauner, Martina and Foessl, Ines and Formosa, Melissa and Kague, Erika and Prijatelj, Vid and Alonso Lopez, Nerea and Banerjee, Bodhisattwa and Bergen, Dylan and Busse, Björn and Calado, Angelo and Douni, Eleni and Gabet, Yankel and Garcı´a Giralt, Natalia and Grinberg, Daniel and Lovsin, Nika and Nogues Solan, Xavier and Ostanek, Barbara and Pavlos, Nathan and Rivadeneira, Fernando and Soldatović, Ivan and van de Peppel, Jeroen and van der Eerden, Bram and van Hul, Wim and Balcells, Susanna and Marc, Janja and Reppe, Sjur and Søe, Kent and Karasik, David",
year = "2021",
abstract = "The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques",
volume = "12",
doi = "10.3389/fendo.2021.731217"
}

Rauner, M., Foessl, I., Formosa, M., Kague, E., Prijatelj, V., Alonso Lopez, N., Banerjee, B., Bergen, D., Busse, B., Calado, A., Douni, E., Gabet, Y., Garcı´a Giralt, N., Grinberg, D., Lovsin, N., Nogues Solan, X., Ostanek, B., Pavlos, N., Rivadeneira, F., Soldatović, I., van de Peppel, J., van der Eerden, B., van Hul, W., Balcells, S., Marc, J., Reppe, S., Søe, K.,& Karasik, D.. (2021). Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology
Frontiers Media S.A.., 12.
https://doi.org/10.3389/fendo.2021.731217

Rauner M, Foessl I, Formosa M, Kague E, Prijatelj V, Alonso Lopez N, Banerjee B, Bergen D, Busse B, Calado A, Douni E, Gabet Y, Garcı´a Giralt N, Grinberg D, Lovsin N, Nogues Solan X, Ostanek B, Pavlos N, Rivadeneira F, Soldatović I, van de Peppel J, van der Eerden B, van Hul W, Balcells S, Marc J, Reppe S, Søe K, Karasik D. Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology. 2021;12.
doi:10.3389/fendo.2021.731217 .

Rauner, Martina, Foessl, Ines, Formosa, Melissa, Kague, Erika, Prijatelj, Vid, Alonso Lopez, Nerea, Banerjee, Bodhisattwa, Bergen, Dylan, Busse, Björn, Calado, Angelo, Douni, Eleni, Gabet, Yankel, Garcı´a Giralt, Natalia, Grinberg, Daniel, Lovsin, Nika, Nogues Solan, Xavier, Ostanek, Barbara, Pavlos, Nathan, Rivadeneira, Fernando, Soldatović, Ivan, van de Peppel, Jeroen, van der Eerden, Bram, van Hul, Wim, Balcells, Susanna, Marc, Janja, Reppe, Sjur, Søe, Kent, Karasik, David, "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques" in Frontiers in Endocrinology, 12 (2021),
https://doi.org/10.3389/fendo.2021.731217 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kocić, Gordana
AU  - Kavarić, Nebojša
AU  - Pavlović, Radmila
AU  - Soldatović, Ivan
AU  - Ninić, Ana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3342
AB  - Background: Nitric oxide (NO) is oxidative stress biomarker which is regarded as one of the key determinants of energy metabolism and vascular tone. Considering the controversial reports on the association between nitric oxide products (NOx) and metabolic syndrome (MetS), the aim of the current study was to examine that potential relationship. Additionally, we aimed to evaluate a broad spectrum of other oxidative stress biomarkers [i.e., malondialdehyde (MDA), advanced oxidation protein products (AOPP), xanthine oxidoreductase (XOD), xanthine oxidase (XO) xanthine dehydrogenase (XDH)] in relation with MetS. Methods: A total of 109 volunteers (46.8% of them with MetS) were included in this cross-sectional study. Biohemical and anthropometric parameters, as well as blood pressure, were obtained. The MetS was diagnosed according to the International Diabetes Federation criteria. Results: Multivariate logistic regression analysis showed that XOD (OR=1.011; 95% CI 1.002-1.019; p=0.016), XO (OR=1.014; 95% CI 1.003-1.026; p=0.016), MDA (OR=1.113; 95% CI 1.038-1.192; p=0.003) and AOPP (OR=1.022; 95% CI 1.005-1.039; p=0.012) were the independent predictors of MetS, whereas no association between NOx and MetS was found. As XOD rose for 1 U/L, XO for 1 U/L, MDA for 1 mu mol/L and AOPP for 1 T/L, probability for MetS rose for 1.1%, 1.4%, 11.3% and 2.2%, respectively. Adjusted R-2 for the Model was 0.531, which means that 53.1% of variation in MetS could be explained with this Model. Conclusions: Unlike XOD, MDA and AOPP, NOx is not associated with MetS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Nitric oxide products are not associated with metabolic syndrome
VL  - 38
IS  - 3
SP  - 361
EP  - 367
DO  - 10.2478/jomb-2018-0035
ER  - 

@article{
author = "Klisić, Aleksandra and Kocić, Gordana and Kavarić, Nebojša and Pavlović, Radmila and Soldatović, Ivan and Ninić, Ana",
year = "2019",
abstract = "Background: Nitric oxide (NO) is oxidative stress biomarker which is regarded as one of the key determinants of energy metabolism and vascular tone. Considering the controversial reports on the association between nitric oxide products (NOx) and metabolic syndrome (MetS), the aim of the current study was to examine that potential relationship. Additionally, we aimed to evaluate a broad spectrum of other oxidative stress biomarkers [i.e., malondialdehyde (MDA), advanced oxidation protein products (AOPP), xanthine oxidoreductase (XOD), xanthine oxidase (XO) xanthine dehydrogenase (XDH)] in relation with MetS. Methods: A total of 109 volunteers (46.8% of them with MetS) were included in this cross-sectional study. Biohemical and anthropometric parameters, as well as blood pressure, were obtained. The MetS was diagnosed according to the International Diabetes Federation criteria. Results: Multivariate logistic regression analysis showed that XOD (OR=1.011; 95% CI 1.002-1.019; p=0.016), XO (OR=1.014; 95% CI 1.003-1.026; p=0.016), MDA (OR=1.113; 95% CI 1.038-1.192; p=0.003) and AOPP (OR=1.022; 95% CI 1.005-1.039; p=0.012) were the independent predictors of MetS, whereas no association between NOx and MetS was found. As XOD rose for 1 U/L, XO for 1 U/L, MDA for 1 mu mol/L and AOPP for 1 T/L, probability for MetS rose for 1.1%, 1.4%, 11.3% and 2.2%, respectively. Adjusted R-2 for the Model was 0.531, which means that 53.1% of variation in MetS could be explained with this Model. Conclusions: Unlike XOD, MDA and AOPP, NOx is not associated with MetS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Nitric oxide products are not associated with metabolic syndrome",
volume = "38",
number = "3",
pages = "361-367",
doi = "10.2478/jomb-2018-0035"
}

Klisić, A., Kocić, G., Kavarić, N., Pavlović, R., Soldatović, I.,& Ninić, A.. (2019). Nitric oxide products are not associated with metabolic syndrome. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 38(3), 361-367.
https://doi.org/10.2478/jomb-2018-0035

Klisić A, Kocić G, Kavarić N, Pavlović R, Soldatović I, Ninić A. Nitric oxide products are not associated with metabolic syndrome. in Journal of Medical Biochemistry. 2019;38(3):361-367.
doi:10.2478/jomb-2018-0035 .

Klisić, Aleksandra, Kocić, Gordana, Kavarić, Nebojša, Pavlović, Radmila, Soldatović, Ivan, Ninić, Ana, "Nitric oxide products are not associated with metabolic syndrome" in Journal of Medical Biochemistry, 38, no. 3 (2019):361-367,
https://doi.org/10.2478/jomb-2018-0035 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Soldatović, Ivan
AU  - Ninić, Ana
AU  - Kotur-Stevuljević, Jelena
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3306
AB  - Background: Retinol-binding protein 4 (RBP4) and cystatin C are regarded as novel metabolic risk markers. Therefore, we aimed to examine which one of these biomarkers better correlates with metabolic syndrome (MetS) in a cohort of postmenopausal women. Methods: A total of 129 postmenopausal women (among which 62 women had MetS) were recruited in this cross-sectional study. MetS was diagnosed according to the International Diabetes Federation criteria. Results: Cystatin C and RBP4 levels were significantly higher in women with MetS, compared to those without MetS (p = 0.011 vs. p  lt 0.001, respectively). A significant difference in the proportion of women with and without MetS across cystatin C and RBP4 quartiles was observed (chi(2) = 5.1, p = 0.025, and chi(2) =11.1, p = 0.001, respectively). Logistic regression analysis revealed a borderline significant relationship between cystatin C and MetS (p = 0.066), but this significance disappeared after adjustment for age, inflammation level and duration of menopause (p = 0.221). On the contrary, a significant relationship between RBP4 and MetS was observed not only without adjustment (p = 0.009), but also even after adjustment for age, inflammation level and duration of menopause (p= 0.006). Conclusions: RBP4 better correlates with MetS than cystatin C in postmenopausal women.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Journal of Laboratory Medicine
T1  - Retinol-binding protein 4 better correlates with metabolic syndrome than cystatin C
VL  - 43
IS  - 1
SP  - 29
EP  - 34
DO  - 10.1515/labmed-2018-0325
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Soldatović, Ivan and Ninić, Ana and Kotur-Stevuljević, Jelena",
year = "2019",
abstract = "Background: Retinol-binding protein 4 (RBP4) and cystatin C are regarded as novel metabolic risk markers. Therefore, we aimed to examine which one of these biomarkers better correlates with metabolic syndrome (MetS) in a cohort of postmenopausal women. Methods: A total of 129 postmenopausal women (among which 62 women had MetS) were recruited in this cross-sectional study. MetS was diagnosed according to the International Diabetes Federation criteria. Results: Cystatin C and RBP4 levels were significantly higher in women with MetS, compared to those without MetS (p = 0.011 vs. p  lt 0.001, respectively). A significant difference in the proportion of women with and without MetS across cystatin C and RBP4 quartiles was observed (chi(2) = 5.1, p = 0.025, and chi(2) =11.1, p = 0.001, respectively). Logistic regression analysis revealed a borderline significant relationship between cystatin C and MetS (p = 0.066), but this significance disappeared after adjustment for age, inflammation level and duration of menopause (p = 0.221). On the contrary, a significant relationship between RBP4 and MetS was observed not only without adjustment (p = 0.009), but also even after adjustment for age, inflammation level and duration of menopause (p= 0.006). Conclusions: RBP4 better correlates with MetS than cystatin C in postmenopausal women.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Journal of Laboratory Medicine",
title = "Retinol-binding protein 4 better correlates with metabolic syndrome than cystatin C",
volume = "43",
number = "1",
pages = "29-34",
doi = "10.1515/labmed-2018-0325"
}

Klisić, A., Kavarić, N., Soldatović, I., Ninić, A.,& Kotur-Stevuljević, J.. (2019). Retinol-binding protein 4 better correlates with metabolic syndrome than cystatin C. in Journal of Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 43(1), 29-34.
https://doi.org/10.1515/labmed-2018-0325

Klisić A, Kavarić N, Soldatović I, Ninić A, Kotur-Stevuljević J. Retinol-binding protein 4 better correlates with metabolic syndrome than cystatin C. in Journal of Laboratory Medicine. 2019;43(1):29-34.
doi:10.1515/labmed-2018-0325 .

Klisić, Aleksandra, Kavarić, Nebojša, Soldatović, Ivan, Ninić, Ana, Kotur-Stevuljević, Jelena, "Retinol-binding protein 4 better correlates with metabolic syndrome than cystatin C" in Journal of Laboratory Medicine, 43, no. 1 (2019):29-34,
https://doi.org/10.1515/labmed-2018-0325 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Bjelaković, Bojko
AU  - Zvrko, Elvir
AU  - Soldatović, Ivan
AU  - Kotur-Stevuljević, Jelena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3120
AB  - Objective: Better than simple anthropometric parameters, the visceral adiposity index (VAI) has recently been proposed as a predictor of cardiometabolic risk in adults. However, there are conflicting results on the associations of these parameters in children and adolescents. Therefore, we aimed to estimate this potential relationship between VAI, anthropometric parameters (i.e., body mass index [BMI], waist circumference [WC], and waist-to-height ratio [WHtR], respectively), and inflammation as measured by high-sensitivity C-reactive protein (hs-CRP) levels in a cohort of adolescent girls. Methods: A total of 90 adolescent girls from 16 to 19 years old were included in cross-sectional study. Anthropometric and biochemical parameters (glucose, lipid parameters, and hsCRP) were measured. The VAI, derived from anthropometric and lipid parameters, calculated {[WC/36.58 + (1.89 x BMI)] x (triglycerides/0.81) x (1.52/ HDL-cholesterol)} was calculated. Results: A comparison of the receiver operating characteristic (ROC) curves showed that all the curves for the anthropometric parameters (e.g., BMI, WC, WHtR) had excellent discriminatory capability with regard to inflammation level status (low vs. high level) and significantly larger areas under the curve (AUC = 0.885, AUC = 0.863, AUC = 0.860, respectively; P lt 0.001) than the ROC curve for VAI did (AUC = 0.686; P = 0.021). Conclusion: Visceral adiposity index is not superior over anthropometric parameters in relation to inflammation as measured by high sensitivity C-reactive protein in adolescent girls.
PB  - Univ Puerto Rico Medical Sciences Campus, San Juan
T2  - Puerto Rico Health Sciences Journal
T1  - Visceral Adiposity Index is not Superior over Anthropometric Parameters with regards to Inflammation in Healthy Adolescent Girls
VL  - 37
IS  - 4
SP  - 195
EP  - 199
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3120
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Bjelaković, Bojko and Zvrko, Elvir and Soldatović, Ivan and Kotur-Stevuljević, Jelena",
year = "2018",
abstract = "Objective: Better than simple anthropometric parameters, the visceral adiposity index (VAI) has recently been proposed as a predictor of cardiometabolic risk in adults. However, there are conflicting results on the associations of these parameters in children and adolescents. Therefore, we aimed to estimate this potential relationship between VAI, anthropometric parameters (i.e., body mass index [BMI], waist circumference [WC], and waist-to-height ratio [WHtR], respectively), and inflammation as measured by high-sensitivity C-reactive protein (hs-CRP) levels in a cohort of adolescent girls. Methods: A total of 90 adolescent girls from 16 to 19 years old were included in cross-sectional study. Anthropometric and biochemical parameters (glucose, lipid parameters, and hsCRP) were measured. The VAI, derived from anthropometric and lipid parameters, calculated {[WC/36.58 + (1.89 x BMI)] x (triglycerides/0.81) x (1.52/ HDL-cholesterol)} was calculated. Results: A comparison of the receiver operating characteristic (ROC) curves showed that all the curves for the anthropometric parameters (e.g., BMI, WC, WHtR) had excellent discriminatory capability with regard to inflammation level status (low vs. high level) and significantly larger areas under the curve (AUC = 0.885, AUC = 0.863, AUC = 0.860, respectively; P lt 0.001) than the ROC curve for VAI did (AUC = 0.686; P = 0.021). Conclusion: Visceral adiposity index is not superior over anthropometric parameters in relation to inflammation as measured by high sensitivity C-reactive protein in adolescent girls.",
publisher = "Univ Puerto Rico Medical Sciences Campus, San Juan",
journal = "Puerto Rico Health Sciences Journal",
title = "Visceral Adiposity Index is not Superior over Anthropometric Parameters with regards to Inflammation in Healthy Adolescent Girls",
volume = "37",
number = "4",
pages = "195-199",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3120"
}

Klisić, A., Kavarić, N., Bjelaković, B., Zvrko, E., Soldatović, I.,& Kotur-Stevuljević, J.. (2018). Visceral Adiposity Index is not Superior over Anthropometric Parameters with regards to Inflammation in Healthy Adolescent Girls. in Puerto Rico Health Sciences Journal
Univ Puerto Rico Medical Sciences Campus, San Juan., 37(4), 195-199.
https://hdl.handle.net/21.15107/rcub_farfar_3120

Klisić A, Kavarić N, Bjelaković B, Zvrko E, Soldatović I, Kotur-Stevuljević J. Visceral Adiposity Index is not Superior over Anthropometric Parameters with regards to Inflammation in Healthy Adolescent Girls. in Puerto Rico Health Sciences Journal. 2018;37(4):195-199.
https://hdl.handle.net/21.15107/rcub_farfar_3120 .

Klisić, Aleksandra, Kavarić, Nebojša, Bjelaković, Bojko, Zvrko, Elvir, Soldatović, Ivan, Kotur-Stevuljević, Jelena, "Visceral Adiposity Index is not Superior over Anthropometric Parameters with regards to Inflammation in Healthy Adolescent Girls" in Puerto Rico Health Sciences Journal, 37, no. 4 (2018):195-199,
https://hdl.handle.net/21.15107/rcub_farfar_3120 .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Bjelaković, Bojko
AU  - Soldatović, Ivan
AU  - Martinović, Milica
AU  - Kotur-Stevuljević, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3017
AB  - Retinol-binding protein 4 (RBP4) is an emerging risk factor for atherosclerotic disease in adults. However, to our knowledge, there are no studies examining the relationship between RBP4 and cardiovascular risk in young population. Therefore, we aimed to estimate this potential relationship in overweight/obese adolescent girls. Seventy overweight/obese adolescent girls, mean age 17.6 +/- 1.20 years, were included. Anthropometric and biochemical parameters were measured. Cardiovascular risk score (CVRS) was calculated by adding points for each risk factor (e.g., sex, high-density lipoprotein cholesterol (HDL-c), non-HDL-c, smoking, blood pressure and fasting glycemia). According to the risk status, we divided adolescent girls into low, medium and high risk groups (-2  lt = CVRS  lt = 1, 2  lt = CVRS 54 and CVRS >= 5, respectively). We found significantly higher RBP4 in the high risk group as compared with low risk group (p lt 0.001). However, multiple linear regression analysis showed waist circumference (beta=0.257, p=0.031) to be the only independent predictor of higher cardiovascular risk (adjusted R'=0.342, p lt 0.001). In conclusion, RBP4 may be associated with higher cardiovascular risk in overweight/obese adolescent girls, but this association is mediated by abdominal obesity.
PB  - Sestre Milosrdnice Univ Hospital, Zagreb
T2  - Acta Clinica Croatica
T1  - The association between retinol-binding protein 4 and cardiovascular risk score is mediated by waist circumference in overweight/obese adolescent girls
VL  - 56
IS  - 1
SP  - 92
EP  - 98
DO  - 10.20471/acc.2017.56.01.14
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Bjelaković, Bojko and Soldatović, Ivan and Martinović, Milica and Kotur-Stevuljević, Jelena",
year = "2017",
abstract = "Retinol-binding protein 4 (RBP4) is an emerging risk factor for atherosclerotic disease in adults. However, to our knowledge, there are no studies examining the relationship between RBP4 and cardiovascular risk in young population. Therefore, we aimed to estimate this potential relationship in overweight/obese adolescent girls. Seventy overweight/obese adolescent girls, mean age 17.6 +/- 1.20 years, were included. Anthropometric and biochemical parameters were measured. Cardiovascular risk score (CVRS) was calculated by adding points for each risk factor (e.g., sex, high-density lipoprotein cholesterol (HDL-c), non-HDL-c, smoking, blood pressure and fasting glycemia). According to the risk status, we divided adolescent girls into low, medium and high risk groups (-2  lt = CVRS  lt = 1, 2  lt = CVRS 54 and CVRS >= 5, respectively). We found significantly higher RBP4 in the high risk group as compared with low risk group (p lt 0.001). However, multiple linear regression analysis showed waist circumference (beta=0.257, p=0.031) to be the only independent predictor of higher cardiovascular risk (adjusted R'=0.342, p lt 0.001). In conclusion, RBP4 may be associated with higher cardiovascular risk in overweight/obese adolescent girls, but this association is mediated by abdominal obesity.",
publisher = "Sestre Milosrdnice Univ Hospital, Zagreb",
journal = "Acta Clinica Croatica",
title = "The association between retinol-binding protein 4 and cardiovascular risk score is mediated by waist circumference in overweight/obese adolescent girls",
volume = "56",
number = "1",
pages = "92-98",
doi = "10.20471/acc.2017.56.01.14"
}

Klisić, A., Kavarić, N., Bjelaković, B., Soldatović, I., Martinović, M.,& Kotur-Stevuljević, J.. (2017). The association between retinol-binding protein 4 and cardiovascular risk score is mediated by waist circumference in overweight/obese adolescent girls. in Acta Clinica Croatica
Sestre Milosrdnice Univ Hospital, Zagreb., 56(1), 92-98.
https://doi.org/10.20471/acc.2017.56.01.14

Klisić A, Kavarić N, Bjelaković B, Soldatović I, Martinović M, Kotur-Stevuljević J. The association between retinol-binding protein 4 and cardiovascular risk score is mediated by waist circumference in overweight/obese adolescent girls. in Acta Clinica Croatica. 2017;56(1):92-98.
doi:10.20471/acc.2017.56.01.14 .

Klisić, Aleksandra, Kavarić, Nebojša, Bjelaković, Bojko, Soldatović, Ivan, Martinović, Milica, Kotur-Stevuljević, Jelena, "The association between retinol-binding protein 4 and cardiovascular risk score is mediated by waist circumference in overweight/obese adolescent girls" in Acta Clinica Croatica, 56, no. 1 (2017):92-98,
https://doi.org/10.20471/acc.2017.56.01.14 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Jovanović, Milovan
AU  - Soldatović, Ivan
AU  - Gligorović-Barhanović, Najdana
AU  - Kotur-Stevuljević, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2800
AB  - Introduction: Previous studies have examined the correlation between hyperandrogenemia and non-alcoholic fatty liver disease (NAFLD) in women and showed contradictory results. Therefore, we aimed to evaluate the relationship between testosterone level and Fatty Liver Index (FLI), as a surrogate marker for NAFLD, in a cohort of postmenopausal women. Material and methods: A total of 150 postmenopausal women were included in this cross-sectional study. Anthropometric and biochemical parameters, as well as blood pressure, were obtained. Non-alcoholic fatty liver disease is assessed by FLI, an algorithm based on body mass index, waist circumference, triglycerides and gamma-glutamyl transferase, as a simple and accurate predictor of hepatic steatosis. Women were divided into three groups (FLI  lt  30, n = 80; 30 = FLI  lt  60, n = 44; FLI = 60, n = 26). Homeostasis model assessment of insulin resistance (HOMA-IR) as a surrogate marker of insulin resistance was calculated. Results: Multiple linear regression analysis revealed that the best model consisted of 4 parameters (e.g., bioavailable testosterone (beta = 0.288, p = 0.001), log HOMA-IR (beta = 0.227, p = 0.005), log high-sensitivity C-reactive protein (beta = 0.322, p  lt  0.001), and retinol-binding protein 4 (beta = 0.226, p  lt  0.001)). Adjusted R-2 for the best model was 0.550, which means that as much as 55.0% of variation in FLI could be explained with this model. Conclusions: Bioavailable testosterone is independently associated with FLI in postmenopausal women.
PB  - Termedia Publishing House Ltd, Poznan
T2  - Archives of Medical Science
T1  - Bioavailable testosterone is independently associated with Fatty Liver Index in postmenopausal women
VL  - 13
IS  - 5
SP  - 1188
EP  - 1196
DO  - 10.5114/aoms.2017.68972
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Jovanović, Milovan and Soldatović, Ivan and Gligorović-Barhanović, Najdana and Kotur-Stevuljević, Jelena",
year = "2017",
abstract = "Introduction: Previous studies have examined the correlation between hyperandrogenemia and non-alcoholic fatty liver disease (NAFLD) in women and showed contradictory results. Therefore, we aimed to evaluate the relationship between testosterone level and Fatty Liver Index (FLI), as a surrogate marker for NAFLD, in a cohort of postmenopausal women. Material and methods: A total of 150 postmenopausal women were included in this cross-sectional study. Anthropometric and biochemical parameters, as well as blood pressure, were obtained. Non-alcoholic fatty liver disease is assessed by FLI, an algorithm based on body mass index, waist circumference, triglycerides and gamma-glutamyl transferase, as a simple and accurate predictor of hepatic steatosis. Women were divided into three groups (FLI  lt  30, n = 80; 30 = FLI  lt  60, n = 44; FLI = 60, n = 26). Homeostasis model assessment of insulin resistance (HOMA-IR) as a surrogate marker of insulin resistance was calculated. Results: Multiple linear regression analysis revealed that the best model consisted of 4 parameters (e.g., bioavailable testosterone (beta = 0.288, p = 0.001), log HOMA-IR (beta = 0.227, p = 0.005), log high-sensitivity C-reactive protein (beta = 0.322, p  lt  0.001), and retinol-binding protein 4 (beta = 0.226, p  lt  0.001)). Adjusted R-2 for the best model was 0.550, which means that as much as 55.0% of variation in FLI could be explained with this model. Conclusions: Bioavailable testosterone is independently associated with FLI in postmenopausal women.",
publisher = "Termedia Publishing House Ltd, Poznan",
journal = "Archives of Medical Science",
title = "Bioavailable testosterone is independently associated with Fatty Liver Index in postmenopausal women",
volume = "13",
number = "5",
pages = "1188-1196",
doi = "10.5114/aoms.2017.68972"
}

Klisić, A., Kavarić, N., Jovanović, M., Soldatović, I., Gligorović-Barhanović, N.,& Kotur-Stevuljević, J.. (2017). Bioavailable testosterone is independently associated with Fatty Liver Index in postmenopausal women. in Archives of Medical Science
Termedia Publishing House Ltd, Poznan., 13(5), 1188-1196.
https://doi.org/10.5114/aoms.2017.68972

Klisić A, Kavarić N, Jovanović M, Soldatović I, Gligorović-Barhanović N, Kotur-Stevuljević J. Bioavailable testosterone is independently associated with Fatty Liver Index in postmenopausal women. in Archives of Medical Science. 2017;13(5):1188-1196.
doi:10.5114/aoms.2017.68972 .

Klisić, Aleksandra, Kavarić, Nebojša, Jovanović, Milovan, Soldatović, Ivan, Gligorović-Barhanović, Najdana, Kotur-Stevuljević, Jelena, "Bioavailable testosterone is independently associated with Fatty Liver Index in postmenopausal women" in Archives of Medical Science, 13, no. 5 (2017):1188-1196,
https://doi.org/10.5114/aoms.2017.68972 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Soldatović, Ivan
AU  - Bjelaković, Bojko
AU  - Kotur-Stevuljević, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2661
AB  - Background: Since the cardiovascular (CV) risk score in the young population, children and adolescents, is underestimated, especially in developing countries such as Montenegro, where a strong interaction exists between the genetically conditioned CV risk and environmental factors, the purpose of this study was to estimate CV risk in apparently healthy adolescent girls. Moreover, we aimed to test some new, emerging CV risk factors and their interaction with the traditional ones, such as obesity. Precisely, we aimed to assess the impact of low bilirubin levels, as a routine biochemical parameter, as an additional risk factor for atherosclerotic disease in the adult phase. Methods: Forty-five obese adolescent girls (mean age 17.8 +/- 1.22 years) and forty-five age-and sex-matched normal weight controls, all nonsmokers, were included. Anthropometric and biochemical parameters were measured. Cardiovascular Risk Score (CVRS) was calculated by adding the points for each risk factor (e.g. sex, HDL-c, non-HDLc, blood pressure and fasting glycemia). Results: A significant positive relationship between CVRS and ALT, hsCRP and TG/HDL-c, but an opposite relationship between CVRS and total bilirubin were found (P lt 0.001). Multiple linear regression analysis showed that higher waist circumference (WC) and LDL-c, but lower HDL-c were independent predictors of lower bilirubin values (adjusted R-2=0.603, P lt 0.001). Conclusions: Obese adolescent girls are at an increased risk of cardiovascular disease late in life. In addition to the traditional risk factors, total bilirubin may have the potential to discriminate between low and higher risk for cardiovascular disturbances in healthy adolescent girls.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Relationship between cardiovascular risk score and traditional and nontraditional cardiometabolic parameters in obese adolescent girls
VL  - 35
IS  - 3
SP  - 282
EP  - 292
DO  - 10.1515/jomb-2016-0005
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Soldatović, Ivan and Bjelaković, Bojko and Kotur-Stevuljević, Jelena",
year = "2016",
abstract = "Background: Since the cardiovascular (CV) risk score in the young population, children and adolescents, is underestimated, especially in developing countries such as Montenegro, where a strong interaction exists between the genetically conditioned CV risk and environmental factors, the purpose of this study was to estimate CV risk in apparently healthy adolescent girls. Moreover, we aimed to test some new, emerging CV risk factors and their interaction with the traditional ones, such as obesity. Precisely, we aimed to assess the impact of low bilirubin levels, as a routine biochemical parameter, as an additional risk factor for atherosclerotic disease in the adult phase. Methods: Forty-five obese adolescent girls (mean age 17.8 +/- 1.22 years) and forty-five age-and sex-matched normal weight controls, all nonsmokers, were included. Anthropometric and biochemical parameters were measured. Cardiovascular Risk Score (CVRS) was calculated by adding the points for each risk factor (e.g. sex, HDL-c, non-HDLc, blood pressure and fasting glycemia). Results: A significant positive relationship between CVRS and ALT, hsCRP and TG/HDL-c, but an opposite relationship between CVRS and total bilirubin were found (P lt 0.001). Multiple linear regression analysis showed that higher waist circumference (WC) and LDL-c, but lower HDL-c were independent predictors of lower bilirubin values (adjusted R-2=0.603, P lt 0.001). Conclusions: Obese adolescent girls are at an increased risk of cardiovascular disease late in life. In addition to the traditional risk factors, total bilirubin may have the potential to discriminate between low and higher risk for cardiovascular disturbances in healthy adolescent girls.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Relationship between cardiovascular risk score and traditional and nontraditional cardiometabolic parameters in obese adolescent girls",
volume = "35",
number = "3",
pages = "282-292",
doi = "10.1515/jomb-2016-0005"
}

Klisić, A., Kavarić, N., Soldatović, I., Bjelaković, B.,& Kotur-Stevuljević, J.. (2016). Relationship between cardiovascular risk score and traditional and nontraditional cardiometabolic parameters in obese adolescent girls. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(3), 282-292.
https://doi.org/10.1515/jomb-2016-0005

Klisić A, Kavarić N, Soldatović I, Bjelaković B, Kotur-Stevuljević J. Relationship between cardiovascular risk score and traditional and nontraditional cardiometabolic parameters in obese adolescent girls. in Journal of Medical Biochemistry. 2016;35(3):282-292.
doi:10.1515/jomb-2016-0005 .

Klisić, Aleksandra, Kavarić, Nebojša, Soldatović, Ivan, Bjelaković, Bojko, Kotur-Stevuljević, Jelena, "Relationship between cardiovascular risk score and traditional and nontraditional cardiometabolic parameters in obese adolescent girls" in Journal of Medical Biochemistry, 35, no. 3 (2016):282-292,
https://doi.org/10.1515/jomb-2016-0005 . .

TY  - JOUR
AU  - Krnjeta, Tijana
AU  - Mirković, Ljiljana
AU  - Ignjatović, Svetlana
AU  - Tomasević, Dragana
AU  - Lukić, Jelena
AU  - Topalov, Drina
AU  - Soldatović, Ivan
AU  - Majkić-Singh, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2638
AB  - Background: Up until now there have been contradictory data about the association between p.Val158Met catechol-O-methyltransferase (COMT) polymorphism and risk of preeclampsia (PE). The goal of this study was to assess the potential correlation between p.Val158Met COMT polymorphism and risk of early-onset PE, risk of a severe form of early-onset PE, as well as risk of small-for-gestationalage (SGA) complicating PE. Methods: The study included 47 early-onset PE patients and 47 control cases. Forty-seven early-onset PE patients were grouped by disease severity (33 patients with a severe form and 14 patients without severe features) and secondly by size for gestational age (12 patients with appropriate-for-gestational-age (AGA) and 35 patients with SGA size). p.Val158Met polymorphism was genotyped by PCR-RFLP analysis. Results: Allele analysis showed significant difference in COMT allele distribution between early-onset PE and control group as well as early-onset PE SGA and controls (p=0.04057 and p=0.0411 respectively). A statistically significant distribution difference between the severe form and form without severe features of early-onset PE patients was not observed (p>0.05). The highest difference ob served was in the allele recessive model where COMT MetMet genotype was associated with decreased risk of early-onset PE (OR=0.281; 95% CI=0.092-0.7836) and PE complications including severe early-onset PE (OR=0.304; 95% CI=0.086-0.944) and SGA early-onset PE (OR=0.284; 95% CI=0.081-0.874). Conclusions: COMT may be used as a candidate gene for early-onset PE and its severe form and SGA complications.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications
VL  - 35
IS  - 3
SP  - 312
EP  - 318
DO  - 10.1515/jomb-2016-0013
ER  - 

@article{
author = "Krnjeta, Tijana and Mirković, Ljiljana and Ignjatović, Svetlana and Tomasević, Dragana and Lukić, Jelena and Topalov, Drina and Soldatović, Ivan and Majkić-Singh, Nada",
year = "2016",
abstract = "Background: Up until now there have been contradictory data about the association between p.Val158Met catechol-O-methyltransferase (COMT) polymorphism and risk of preeclampsia (PE). The goal of this study was to assess the potential correlation between p.Val158Met COMT polymorphism and risk of early-onset PE, risk of a severe form of early-onset PE, as well as risk of small-for-gestationalage (SGA) complicating PE. Methods: The study included 47 early-onset PE patients and 47 control cases. Forty-seven early-onset PE patients were grouped by disease severity (33 patients with a severe form and 14 patients without severe features) and secondly by size for gestational age (12 patients with appropriate-for-gestational-age (AGA) and 35 patients with SGA size). p.Val158Met polymorphism was genotyped by PCR-RFLP analysis. Results: Allele analysis showed significant difference in COMT allele distribution between early-onset PE and control group as well as early-onset PE SGA and controls (p=0.04057 and p=0.0411 respectively). A statistically significant distribution difference between the severe form and form without severe features of early-onset PE patients was not observed (p>0.05). The highest difference ob served was in the allele recessive model where COMT MetMet genotype was associated with decreased risk of early-onset PE (OR=0.281; 95% CI=0.092-0.7836) and PE complications including severe early-onset PE (OR=0.304; 95% CI=0.086-0.944) and SGA early-onset PE (OR=0.284; 95% CI=0.081-0.874). Conclusions: COMT may be used as a candidate gene for early-onset PE and its severe form and SGA complications.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications",
volume = "35",
number = "3",
pages = "312-318",
doi = "10.1515/jomb-2016-0013"
}

Krnjeta, T., Mirković, L., Ignjatović, S., Tomasević, D., Lukić, J., Topalov, D., Soldatović, I.,& Majkić-Singh, N.. (2016). Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(3), 312-318.
https://doi.org/10.1515/jomb-2016-0013

Krnjeta T, Mirković L, Ignjatović S, Tomasević D, Lukić J, Topalov D, Soldatović I, Majkić-Singh N. Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications. in Journal of Medical Biochemistry. 2016;35(3):312-318.
doi:10.1515/jomb-2016-0013 .

Krnjeta, Tijana, Mirković, Ljiljana, Ignjatović, Svetlana, Tomasević, Dragana, Lukić, Jelena, Topalov, Drina, Soldatović, Ivan, Majkić-Singh, Nada, "Protective role of maternal P.VAL158MET catechol-o-methyltransferase polymorphism against early-onset preeclampsia and its complications" in Journal of Medical Biochemistry, 35, no. 3 (2016):312-318,
https://doi.org/10.1515/jomb-2016-0013 . .

TY  - CONF
AU  - Dimitrijević-Srećković, Vesna
AU  - Srecković, B.
AU  - Janeski, H.
AU  - Soldatović, Ivan
AU  - Janać, Jelena
AU  - Jelić-Ivanović, Zorana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2444
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis
T1  - Abdominal obesity responsible for depression and risk of early atherosclerosis in adolescents and youth
VL  - 241
IS  - 1
SP  - e167
EP  - e167
DO  - 10.1016/j.atherosclerosis.2015.04.859
ER  - 

@conference{
author = "Dimitrijević-Srećković, Vesna and Srecković, B. and Janeski, H. and Soldatović, Ivan and Janać, Jelena and Jelić-Ivanović, Zorana",
year = "2015",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Abdominal obesity responsible for depression and risk of early atherosclerosis in adolescents and youth",
volume = "241",
number = "1",
pages = "e167-e167",
doi = "10.1016/j.atherosclerosis.2015.04.859"
}

Dimitrijević-Srećković, V., Srecković, B., Janeski, H., Soldatović, I., Janać, J.,& Jelić-Ivanović, Z.. (2015). Abdominal obesity responsible for depression and risk of early atherosclerosis in adolescents and youth. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 241(1), e167-e167.
https://doi.org/10.1016/j.atherosclerosis.2015.04.859

Dimitrijević-Srećković V, Srecković B, Janeski H, Soldatović I, Janać J, Jelić-Ivanović Z. Abdominal obesity responsible for depression and risk of early atherosclerosis in adolescents and youth. in Atherosclerosis. 2015;241(1):e167-e167.
doi:10.1016/j.atherosclerosis.2015.04.859 .

Dimitrijević-Srećković, Vesna, Srecković, B., Janeski, H., Soldatović, Ivan, Janać, Jelena, Jelić-Ivanović, Zorana, "Abdominal obesity responsible for depression and risk of early atherosclerosis in adolescents and youth" in Atherosclerosis, 241, no. 1 (2015):e167-e167,
https://doi.org/10.1016/j.atherosclerosis.2015.04.859 . .

TY  - CONF
AU  - Dimitrijević-Srećković, Vesna
AU  - Janeski, H.
AU  - Srecković, B.
AU  - Soldatović, Ivan
AU  - Janać, Jelena
AU  - Jelić-Ivanović, Zorana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2447
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis
T1  - Progression of abdominal obesity in youth followed by testosterone decrease leading to erectile dysfunction and risk of early atherosclerosis
VL  - 241
IS  - 1
SP  - e167
EP  - e167
DO  - 10.1016/j.atherosclerosis.2015.04.860
ER  - 

@conference{
author = "Dimitrijević-Srećković, Vesna and Janeski, H. and Srecković, B. and Soldatović, Ivan and Janać, Jelena and Jelić-Ivanović, Zorana",
year = "2015",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Progression of abdominal obesity in youth followed by testosterone decrease leading to erectile dysfunction and risk of early atherosclerosis",
volume = "241",
number = "1",
pages = "e167-e167",
doi = "10.1016/j.atherosclerosis.2015.04.860"
}

Dimitrijević-Srećković, V., Janeski, H., Srecković, B., Soldatović, I., Janać, J.,& Jelić-Ivanović, Z.. (2015). Progression of abdominal obesity in youth followed by testosterone decrease leading to erectile dysfunction and risk of early atherosclerosis. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 241(1), e167-e167.
https://doi.org/10.1016/j.atherosclerosis.2015.04.860

Dimitrijević-Srećković V, Janeski H, Srecković B, Soldatović I, Janać J, Jelić-Ivanović Z. Progression of abdominal obesity in youth followed by testosterone decrease leading to erectile dysfunction and risk of early atherosclerosis. in Atherosclerosis. 2015;241(1):e167-e167.
doi:10.1016/j.atherosclerosis.2015.04.860 .

Dimitrijević-Srećković, Vesna, Janeski, H., Srecković, B., Soldatović, Ivan, Janać, Jelena, Jelić-Ivanović, Zorana, "Progression of abdominal obesity in youth followed by testosterone decrease leading to erectile dysfunction and risk of early atherosclerosis" in Atherosclerosis, 241, no. 1 (2015):e167-e167,
https://doi.org/10.1016/j.atherosclerosis.2015.04.860 . .