TY  - JOUR
AU  - Milanović, Z
AU  - Vekić, Jelena
AU  - Radonjić, V
AU  - Ilić-Božović, A
AU  - Zeljković, Aleksandra
AU  - Janać, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Buch, J
AU  - Chandrashekar, R
AU  - Bojić-Trbojević, Ž
AU  - Hajduković, L
AU  - Christopher, M.M
AU  - Kovačević Filipović, M
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3253
AB  - Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P  lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P  lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
PB  - Blackwell Publishing Inc.
T2  - Journal of Veterinary Internal Medicine
T1  - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
VL  - 33
IS  - 4
SP  - 1686
EP  - 1694
DO  - 10.1111/jvim.15537
ER  - 

@article{
author = "Milanović, Z and Vekić, Jelena and Radonjić, V and Ilić-Božović, A and Zeljković, Aleksandra and Janać, Jelena and Spasojević-Kalimanovska, Vesna and Buch, J and Chandrashekar, R and Bojić-Trbojević, Ž and Hajduković, L and Christopher, M.M and Kovačević Filipović, M",
year = "2019",
abstract = "Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P  lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P  lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
publisher = "Blackwell Publishing Inc.",
journal = "Journal of Veterinary Internal Medicine",
title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs",
volume = "33",
number = "4",
pages = "1686-1694",
doi = "10.1111/jvim.15537"
}

Milanović, Z., Vekić, J., Radonjić, V., Ilić-Božović, A., Zeljković, A., Janać, J., Spasojević-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojić-Trbojević, Ž., Hajduković, L., Christopher, M.M,& Kovačević Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine
Blackwell Publishing Inc.., 33(4), 1686-1694.
https://doi.org/10.1111/jvim.15537

Milanović Z, Vekić J, Radonjić V, Ilić-Božović A, Zeljković A, Janać J, Spasojević-Kalimanovska V, Buch J, Chandrashekar R, Bojić-Trbojević Ž, Hajduković L, Christopher M, Kovačević Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694.
doi:10.1111/jvim.15537 .

Milanović, Z, Vekić, Jelena, Radonjić, V, Ilić-Božović, A, Zeljković, Aleksandra, Janać, Jelena, Spasojević-Kalimanovska, Vesna, Buch, J, Chandrashekar, R, Bojić-Trbojević, Ž, Hajduković, L, Christopher, M.M, Kovačević Filipović, M, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694,
https://doi.org/10.1111/jvim.15537 . .