TY  - JOUR
AU  - Rmandić, Milena
AU  - Stajić, Ana
AU  - Jančić, Jasna
AU  - Samardžić, Janko
AU  - Jović, Nebojša
AU  - Malenović, Anđelija
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4262
AB  - In this research, a UHPLC–MS/MS method was developed and validated for the deter-
mination of zonisamide in dried plasma spots (DPS) and dried blood spots (DBS). Detection of
zonisamide and internal standard, 1-(2,3-dichlorphenyl)piperazine, was carried out in ESI+ mode by
monitoring two MRM transitions per analyte. Total run time, less than 2.5 min, was achieved using
Acquity UPLC BEH Amide (2.1 × 100 mm, 1.7 μm particle size) column with mobile phase com-
prising acetonitrile–water (85:15%, v/v) with 0.075% formic acid. The flow rate was 0.225 mL/min,
the column temperature was 30 ◦C and the injection volume was 3 μL. Desolvation temperature,
desolvation gas flow rate, ion source temperature and cone gas flow rate were set by the IntelliStart
software tool in combination with tuning. All of the Guthrie cards were scanned, and DPS/DBS
areas were determined by the image processing tool. The influence of hematocrit values (20–60%)
on accuracy and precision was evaluated to determine the range within which method for DBSs is
free from Hct or dependency is within acceptable limits. The validated method was applied to the
determination of zonisamide levels in DPS and DBS samples obtained from patients confirming its
suitability for clinical application. Finally, the distribution of zonisamide into the red blood cells was
estimated by correlating its DPS and DBS levels.
PB  - MDPI
T2  - Molecules
T1  - Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice
VL  - 27
IS  - 15
DO  - 10.3390/molecules27154899
ER  - 

@article{
author = "Rmandić, Milena and Stajić, Ana and Jančić, Jasna and Samardžić, Janko and Jović, Nebojša and Malenović, Anđelija",
year = "2022",
abstract = "In this research, a UHPLC–MS/MS method was developed and validated for the deter-
mination of zonisamide in dried plasma spots (DPS) and dried blood spots (DBS). Detection of
zonisamide and internal standard, 1-(2,3-dichlorphenyl)piperazine, was carried out in ESI+ mode by
monitoring two MRM transitions per analyte. Total run time, less than 2.5 min, was achieved using
Acquity UPLC BEH Amide (2.1 × 100 mm, 1.7 μm particle size) column with mobile phase com-
prising acetonitrile–water (85:15%, v/v) with 0.075% formic acid. The flow rate was 0.225 mL/min,
the column temperature was 30 ◦C and the injection volume was 3 μL. Desolvation temperature,
desolvation gas flow rate, ion source temperature and cone gas flow rate were set by the IntelliStart
software tool in combination with tuning. All of the Guthrie cards were scanned, and DPS/DBS
areas were determined by the image processing tool. The influence of hematocrit values (20–60%)
on accuracy and precision was evaluated to determine the range within which method for DBSs is
free from Hct or dependency is within acceptable limits. The validated method was applied to the
determination of zonisamide levels in DPS and DBS samples obtained from patients confirming its
suitability for clinical application. Finally, the distribution of zonisamide into the red blood cells was
estimated by correlating its DPS and DBS levels.",
publisher = "MDPI",
journal = "Molecules",
title = "Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice",
volume = "27",
number = "15",
doi = "10.3390/molecules27154899"
}

Rmandić, M., Stajić, A., Jančić, J., Samardžić, J., Jović, N.,& Malenović, A.. (2022). Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice. in Molecules
MDPI., 27(15).
https://doi.org/10.3390/molecules27154899

Rmandić M, Stajić A, Jančić J, Samardžić J, Jović N, Malenović A. Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice. in Molecules. 2022;27(15).
doi:10.3390/molecules27154899 .

Rmandić, Milena, Stajić, Ana, Jančić, Jasna, Samardžić, Janko, Jović, Nebojša, Malenović, Anđelija, "Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice" in Molecules, 27, no. 15 (2022),
https://doi.org/10.3390/molecules27154899 . .

TY  - JOUR
AU  - Kostić, Nada
AU  - Dotsikas, Yannis
AU  - Jović, Nebojša
AU  - Stevanović, Galina
AU  - Malenović, Anđelija
AU  - Medenica, Mirjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2410
AB  - In this paper, novel LC-MS/MS methods for the determination of antiepileptic drug pregabalin in dried matrix spots (DMS) are presented. This attractive technique of sample collection in micro amount was utilized in the form of dried blood spots (DBS) and dried plasma spots (DPS). Following a pre-column derivatization procedure, using n-propyl chloroformate in the presence of n-propanol, and consecutive liquid-liquid extraction, derivatized pregabalin and its internal standard, 4-aminocyclohexanecarboxylic acid, were detected in positive ion mode by applying two SRM transitions per analyte. A YMC-Pack Octyl column (50 mm x 4.0 mm, 3 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 2 min. Established methods were fully validated over the concentration range of 0.200-20.0 mu g/mL for DBS and 0.400-40.0 mu g/mL for DPS, respectively, while specificity, accuracy, precision, recovery, matrix-effect, stability, dilution integrity and spot homogeneity were found within acceptance criteria. Validated methods were applied for the determination of pregabalin levels in dried blood and plasma samples obtained from patients with epilepsy, after per os administration of commercial capsules. Comparison of drug level in blood and plasma, as well as correction steps undertaken in order to overcome hematocrit issue, when analyzing DBS, are also given.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods
VL  - 109
SP  - 79
EP  - 84
DO  - 10.1016/j.jpba.2015.02.023
ER  - 

@article{
author = "Kostić, Nada and Dotsikas, Yannis and Jović, Nebojša and Stevanović, Galina and Malenović, Anđelija and Medenica, Mirjana",
year = "2015",
abstract = "In this paper, novel LC-MS/MS methods for the determination of antiepileptic drug pregabalin in dried matrix spots (DMS) are presented. This attractive technique of sample collection in micro amount was utilized in the form of dried blood spots (DBS) and dried plasma spots (DPS). Following a pre-column derivatization procedure, using n-propyl chloroformate in the presence of n-propanol, and consecutive liquid-liquid extraction, derivatized pregabalin and its internal standard, 4-aminocyclohexanecarboxylic acid, were detected in positive ion mode by applying two SRM transitions per analyte. A YMC-Pack Octyl column (50 mm x 4.0 mm, 3 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 2 min. Established methods were fully validated over the concentration range of 0.200-20.0 mu g/mL for DBS and 0.400-40.0 mu g/mL for DPS, respectively, while specificity, accuracy, precision, recovery, matrix-effect, stability, dilution integrity and spot homogeneity were found within acceptance criteria. Validated methods were applied for the determination of pregabalin levels in dried blood and plasma samples obtained from patients with epilepsy, after per os administration of commercial capsules. Comparison of drug level in blood and plasma, as well as correction steps undertaken in order to overcome hematocrit issue, when analyzing DBS, are also given.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods",
volume = "109",
pages = "79-84",
doi = "10.1016/j.jpba.2015.02.023"
}

Kostić, N., Dotsikas, Y., Jović, N., Stevanović, G., Malenović, A.,& Medenica, M.. (2015). Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 109, 79-84.
https://doi.org/10.1016/j.jpba.2015.02.023

Kostić N, Dotsikas Y, Jović N, Stevanović G, Malenović A, Medenica M. Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods. in Journal of Pharmaceutical and Biomedical Analysis. 2015;109:79-84.
doi:10.1016/j.jpba.2015.02.023 .

Kostić, Nada, Dotsikas, Yannis, Jović, Nebojša, Stevanović, Galina, Malenović, Anđelija, Medenica, Mirjana, "Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods" in Journal of Pharmaceutical and Biomedical Analysis, 109 (2015):79-84,
https://doi.org/10.1016/j.jpba.2015.02.023 . .

TY  - JOUR
AU  - Kostić, Nada
AU  - Dotsikas, Yannis
AU  - Jović, Nebojša
AU  - Stevanović, Galina
AU  - Malenović, Anđelija
AU  - Medenica, Mirjana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2212
AB  - This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice
VL  - 962
SP  - 102
EP  - 108
DO  - 10.1016/j.jchromb.2014.05.037
ER  - 

@article{
author = "Kostić, Nada and Dotsikas, Yannis and Jović, Nebojša and Stevanović, Galina and Malenović, Anđelija and Medenica, Mirjana",
year = "2014",
abstract = "This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice",
volume = "962",
pages = "102-108",
doi = "10.1016/j.jchromb.2014.05.037"
}

Kostić, N., Dotsikas, Y., Jović, N., Stevanović, G., Malenović, A.,& Medenica, M.. (2014). Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 962, 102-108.
https://doi.org/10.1016/j.jchromb.2014.05.037

Kostić N, Dotsikas Y, Jović N, Stevanović G, Malenović A, Medenica M. Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2014;962:102-108.
doi:10.1016/j.jchromb.2014.05.037 .

Kostić, Nada, Dotsikas, Yannis, Jović, Nebojša, Stevanović, Galina, Malenović, Anđelija, Medenica, Mirjana, "Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 962 (2014):102-108,
https://doi.org/10.1016/j.jchromb.2014.05.037 . .