TY  - JOUR
AU  - Rakić, Tijana
AU  - Jovanović, Marko
AU  - Dumić, Aleksandra
AU  - Pekić, Marina
AU  - Ribić, Sanja
AU  - Jančić-Stojanović, Biljana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1982
AB  - This paper presents multiobjective optimization of complex mixtures separation in hydrophilic interaction liquid chromatography (HILIC). The selected model mixture consisted of five psychotropic drugs: clozapine, thioridazine, sulpiride, pheniramine and lamotrigine. Three factors related to the mobile phase composition (acetonitrile content, pH of the water phase and concentration of ammonium acetate) were optimized in order to achieve the following goals: maximal separation quality, minimal total analysis duration and robustness of an optimum. The consideration of robustness in early phases of the method development provides reliable methods with low risk for failure in validation phase. The simultaneous optimization of all goals was achieved by multiple threshold approach combined with grid point search. The identified optimal separation conditions (acetonitrile content 83%, pH of the water phase 3.5 and ammonium acetate content in water phase 14 mM) were experimentally verified.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Robust Optimization of Psychotropic Drug Mixture Separation in Hydrophilic Interaction Liquid Chromatography
VL  - 60
IS  - 2
SP  - 411
EP  - 415
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1982
ER  - 

@article{
author = "Rakić, Tijana and Jovanović, Marko and Dumić, Aleksandra and Pekić, Marina and Ribić, Sanja and Jančić-Stojanović, Biljana",
year = "2013",
abstract = "This paper presents multiobjective optimization of complex mixtures separation in hydrophilic interaction liquid chromatography (HILIC). The selected model mixture consisted of five psychotropic drugs: clozapine, thioridazine, sulpiride, pheniramine and lamotrigine. Three factors related to the mobile phase composition (acetonitrile content, pH of the water phase and concentration of ammonium acetate) were optimized in order to achieve the following goals: maximal separation quality, minimal total analysis duration and robustness of an optimum. The consideration of robustness in early phases of the method development provides reliable methods with low risk for failure in validation phase. The simultaneous optimization of all goals was achieved by multiple threshold approach combined with grid point search. The identified optimal separation conditions (acetonitrile content 83%, pH of the water phase 3.5 and ammonium acetate content in water phase 14 mM) were experimentally verified.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Robust Optimization of Psychotropic Drug Mixture Separation in Hydrophilic Interaction Liquid Chromatography",
volume = "60",
number = "2",
pages = "411-415",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1982"
}

Rakić, T., Jovanović, M., Dumić, A., Pekić, M., Ribić, S.,& Jančić-Stojanović, B.. (2013). Robust Optimization of Psychotropic Drug Mixture Separation in Hydrophilic Interaction Liquid Chromatography. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 60(2), 411-415.
https://hdl.handle.net/21.15107/rcub_farfar_1982

Rakić T, Jovanović M, Dumić A, Pekić M, Ribić S, Jančić-Stojanović B. Robust Optimization of Psychotropic Drug Mixture Separation in Hydrophilic Interaction Liquid Chromatography. in Acta Chimica Slovenica. 2013;60(2):411-415.
https://hdl.handle.net/21.15107/rcub_farfar_1982 .

Rakić, Tijana, Jovanović, Marko, Dumić, Aleksandra, Pekić, Marina, Ribić, Sanja, Jančić-Stojanović, Biljana, "Robust Optimization of Psychotropic Drug Mixture Separation in Hydrophilic Interaction Liquid Chromatography" in Acta Chimica Slovenica, 60, no. 2 (2013):411-415,
https://hdl.handle.net/21.15107/rcub_farfar_1982 .