TY  - JOUR
AU  - Milašinović, Nikola
AU  - Čalija, Bojan
AU  - Vidović, Bojana
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2607
AB  - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
VL  - 60
SP  - 106
EP  - 112
DO  - 10.1016/j.jtice.2015.10.037
ER  - 

@article{
author = "Milašinović, Nikola and Čalija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica",
year = "2016",
abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method",
volume = "60",
pages = "106-112",
doi = "10.1016/j.jtice.2015.10.037"
}

Milašinović, N., Čalija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science BV, Amsterdam., 60, 106-112.
https://doi.org/10.1016/j.jtice.2015.10.037

Milašinović N, Čalija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers. 2016;60:106-112.
doi:10.1016/j.jtice.2015.10.037 .

Milašinović, Nikola, Čalija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan Institute of Chemical Engineers, 60 (2016):106-112,
https://doi.org/10.1016/j.jtice.2015.10.037 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Kotur-Stevuljević, Jelena
AU  - Dilber, Sanda
AU  - Kalagasidis-Krusić, Melina T.
AU  - Đorđević, Brižita
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2605
AB  - Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity
VL  - 70
IS  - 1
SP  - 49
EP  - 58
DO  - 10.2298/HEMIND141119010V
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Kotur-Stevuljević, Jelena and Dilber, Sanda and Kalagasidis-Krusić, Melina T. and Đorđević, Brižita and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity",
volume = "70",
number = "1",
pages = "49-58",
doi = "10.2298/HEMIND141119010V"
}

Vidović, B., Milašinović, N., Kotur-Stevuljević, J., Dilber, S., Kalagasidis-Krusić, M. T., Đorđević, B.,& Knežević-Jugović, Z.. (2016). Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(1), 49-58.
https://doi.org/10.2298/HEMIND141119010V

Vidović B, Milašinović N, Kotur-Stevuljević J, Dilber S, Kalagasidis-Krusić MT, Đorđević B, Knežević-Jugović Z. Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija. 2016;70(1):49-58.
doi:10.2298/HEMIND141119010V .

Vidović, Bojana, Milašinović, Nikola, Kotur-Stevuljević, Jelena, Dilber, Sanda, Kalagasidis-Krusić, Melina T., Đorđević, Brižita, Knežević-Jugović, Zorica, "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity" in Hemijska industrija, 70, no. 1 (2016):49-58,
https://doi.org/10.2298/HEMIND141119010V . .

TY  - CONF
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Vidović, Jana
AU  - Čalija, Bojan
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Milić, Jela
AU  - Đorđević, Brižita
AU  - Kalagasidis-Krusić, Melina T.
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2333
AB  - Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.
PB  - Karger, Basel
C3  - Annals of Nutrition and Metabolism
T1  - Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity
VL  - 67
IS  - Supplement 1
SP  - 484
EP  - 485
DO  - 10.1159/000440895
ER  - 

@conference{
author = "Vidović, Bojana and Milašinović, Nikola and Vidović, Jana and Čalija, Bojan and Crevar-Sakač, Milkica and Vujić, Zorica and Milić, Jela and Đorđević, Brižita and Kalagasidis-Krusić, Melina T. and Knežević-Jugović, Zorica",
year = "2015",
abstract = "Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity",
volume = "67",
number = "Supplement 1",
pages = "484-485",
doi = "10.1159/000440895"
}

Vidović, B., Milašinović, N., Vidović, J., Čalija, B., Crevar-Sakač, M., Vujić, Z., Milić, J., Đorđević, B., Kalagasidis-Krusić, M. T.,& Knežević-Jugović, Z.. (2015). Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism
Karger, Basel., 67(Supplement 1), 484-485.
https://doi.org/10.1159/000440895

Vidović B, Milašinović N, Vidović J, Čalija B, Crevar-Sakač M, Vujić Z, Milić J, Đorđević B, Kalagasidis-Krusić MT, Knežević-Jugović Z. Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism. 2015;67(Supplement 1):484-485.
doi:10.1159/000440895 .

Vidović, Bojana, Milašinović, Nikola, Vidović, Jana, Čalija, Bojan, Crevar-Sakač, Milkica, Vujić, Zorica, Milić, Jela, Đorđević, Brižita, Kalagasidis-Krusić, Melina T., Knežević-Jugović, Zorica, "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity" in Annals of Nutrition and Metabolism, 67, no. Supplement 1 (2015):484-485,
https://doi.org/10.1159/000440895 . .