TY  - CONF
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Vidović, Jana
AU  - Čalija, Bojan
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Milić, Jela
AU  - Đorđević, Brižita
AU  - Kalagasidis-Krusić, Melina T.
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2333
AB  - Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.
PB  - Karger, Basel
C3  - Annals of Nutrition and Metabolism
T1  - Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity
VL  - 67
IS  - Supplement 1
SP  - 484
EP  - 485
DO  - 10.1159/000440895
ER  - 

@conference{
author = "Vidović, Bojana and Milašinović, Nikola and Vidović, Jana and Čalija, Bojan and Crevar-Sakač, Milkica and Vujić, Zorica and Milić, Jela and Đorđević, Brižita and Kalagasidis-Krusić, Melina T. and Knežević-Jugović, Zorica",
year = "2015",
abstract = "Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity",
volume = "67",
number = "Supplement 1",
pages = "484-485",
doi = "10.1159/000440895"
}

Vidović, B., Milašinović, N., Vidović, J., Čalija, B., Crevar-Sakač, M., Vujić, Z., Milić, J., Đorđević, B., Kalagasidis-Krusić, M. T.,& Knežević-Jugović, Z.. (2015). Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism
Karger, Basel., 67(Supplement 1), 484-485.
https://doi.org/10.1159/000440895

Vidović B, Milašinović N, Vidović J, Čalija B, Crevar-Sakač M, Vujić Z, Milić J, Đorđević B, Kalagasidis-Krusić MT, Knežević-Jugović Z. Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism. 2015;67(Supplement 1):484-485.
doi:10.1159/000440895 .

Vidović, Bojana, Milašinović, Nikola, Vidović, Jana, Čalija, Bojan, Crevar-Sakač, Milkica, Vujić, Zorica, Milić, Jela, Đorđević, Brižita, Kalagasidis-Krusić, Melina T., Knežević-Jugović, Zorica, "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity" in Annals of Nutrition and Metabolism, 67, no. Supplement 1 (2015):484-485,
https://doi.org/10.1159/000440895 . .