TY  - JOUR
AU  - Đurđić, Slađana
AU  - Stanković, Vesna
AU  - Ražić, Slavica
AU  - Mutić, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3983
AB  - Lead isotope ratio pattern (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb) was analyzed in 59 samples of Serbian wine, from four geographical regions. By utilization of powerful inductively coupled plasma mass spectrometry (ICP-QMS), lead isotope ratios were used as unique “fingerprint”, when combined with multivariate methods of analysis (Principal Component Analysis), provided information on the geographical origin of wine. In validation of ICP- QMS method and quantitative analysis, the certified reference material NIST SRM 981 was employed to test the mass-bias correction and thallium isotopes 203Tl and 205Tl (NIST SRM 997) as an internal standard. The obtained results were discussed in correlation with the corresponding values of LIRs of different European and Australian wines. In addition, the impact of anthropogenic Pb from different sources on the total Pb isotopic composition in Serbian wines was analyzed too. On the other side, the obtained values of Pb content were compared with the applicable health safety standards, according to the International Code of Oenological Practices.
PB  - Frontiers Media S.A.
T2  - Frontiers in Chemistry
T1  - Is a Lead Isotope Ratios in Wine Good Marker for Origin Assessment?
VL  - 9
DO  - 10.3389/fchem.2021.746695
ER  - 

@article{
author = "Đurđić, Slađana and Stanković, Vesna and Ražić, Slavica and Mutić, Jelena",
year = "2021",
abstract = "Lead isotope ratio pattern (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb) was analyzed in 59 samples of Serbian wine, from four geographical regions. By utilization of powerful inductively coupled plasma mass spectrometry (ICP-QMS), lead isotope ratios were used as unique “fingerprint”, when combined with multivariate methods of analysis (Principal Component Analysis), provided information on the geographical origin of wine. In validation of ICP- QMS method and quantitative analysis, the certified reference material NIST SRM 981 was employed to test the mass-bias correction and thallium isotopes 203Tl and 205Tl (NIST SRM 997) as an internal standard. The obtained results were discussed in correlation with the corresponding values of LIRs of different European and Australian wines. In addition, the impact of anthropogenic Pb from different sources on the total Pb isotopic composition in Serbian wines was analyzed too. On the other side, the obtained values of Pb content were compared with the applicable health safety standards, according to the International Code of Oenological Practices.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Chemistry",
title = "Is a Lead Isotope Ratios in Wine Good Marker for Origin Assessment?",
volume = "9",
doi = "10.3389/fchem.2021.746695"
}

Đurđić, S., Stanković, V., Ražić, S.,& Mutić, J.. (2021). Is a Lead Isotope Ratios in Wine Good Marker for Origin Assessment?. in Frontiers in Chemistry
Frontiers Media S.A.., 9.
https://doi.org/10.3389/fchem.2021.746695

Đurđić S, Stanković V, Ražić S, Mutić J. Is a Lead Isotope Ratios in Wine Good Marker for Origin Assessment?. in Frontiers in Chemistry. 2021;9.
doi:10.3389/fchem.2021.746695 .

Đurđić, Slađana, Stanković, Vesna, Ražić, Slavica, Mutić, Jelena, "Is a Lead Isotope Ratios in Wine Good Marker for Origin Assessment?" in Frontiers in Chemistry, 9 (2021),
https://doi.org/10.3389/fchem.2021.746695 . .

TY  - CONF
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
AU  - Osmokrović, Andrea
AU  - Mišković Stanković, Vesna
AU  - Jančić, Ivan
AU  - Milenković, Marina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4666
AB  - Alginate hydrogels are widely used in wound dressings due to hydrophilicity, biocompatibility,
flexibility, and high sorption capacity, providing effective moisture regulation in wounds and inducing
rapid granulation and reepithelization of the damaged tissue. However, these dressings are not
bioactive so that different methodologies have been investigated to extend functionality of alginate
hydrogels.
In the present work, we show several approaches to achieve this aim by addition of different
biologically active components. These include incorporation of silver nanoparticles as potent
antimicrobial agents (1), bioactive honey components (2), activated charcoal (AC) particles as carriers
of therapeutically active agents (3) as well as the use of Zn-alginate hydrogels that release zinc ions (4).
The obtained composites were comprehensively characterized regarding composition, cytotoxicity,
antibacterial activity, release kinetics of active agents and wound treatment in a rat model.
Ag/alginate nanocomposite hydrogels releasing silver ions and/or nanoparticles exhibited high
bactericidal activity against a broad spectrum of standard and multi-drug resistant clinical bacterial
strains (Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus – MRSA,
Acinetobacter baumannii and Pseudomonas aeruginosa). Especially interesting results were obtained
against 13 clinical isolates of A. baumannii, which were completely extinguished over 48 h in 6 cases
(2). However, in 3 clinical isolates, antibacterial effects were not noticed implying possibility for
development of bacterial resistance to silver. In the treatment of 2nd degree burns in rats Ag/alginate
nanocomposites exhibited the same efficiency as commercial medical products (5).
Composite alginate hydrogels with immobilized AC particles impregnated with povidone iodine
(PVP-I) as a model therapeutically active agent, were developed with the aim to provide controlled
particle release in the wound without actually releasing the adsorbed substance, thus achieving the
desired activity without adverse effects by systemic absorption. The composite Ca-alginate hydrogels
induced strong bactericidal effects against two standard bacterial strains and clinical multi-resistant
wound isolates (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis and Proteus mirabilis) without
releasing PVP-I in the environment (3). Furthermore, composite Zn-alginate hydrogels released zinc
ions in addition to AC particles with adsorbed PVP-I, which induced additional microbicidal effects on
one wild yeast strain (Candida albicans). The obtained bactericidal effects were ascribed to effective
adsorption of bacteria onto AC particles and further direct contact with the adsorbed iodine, while the
antifungal activity against C. albicans was assigned to released Zn 2+.
Overall, the developed composite alginate hydrogels have shown high potentials for utilization
in variety of multifunctional wound dressings according to the specific needs.
AB  - Alginatni hidrogelovi se široko koriste u oblogama za rane zbog svoje hidrofilnosti,
biokompatibilnosti, fleksibilnosti i velikog sorpcionog kapaciteta čime obezbeđuju efikasnu regulaciju
vlažnosti rane i podstiču brzu granulaciju i reepitelizaciju oštećenog tkiva. Međutim, ove obloge nisu
bioaktivne tako da su istraživane različite metodologije kako bi se proširila funkcionalnost alginatnih
hidrogelova.
U ovom radu je prikazano nekoliko pristupa ostvarivanju tog cilja dodatkom različitih biološki
aktivnih komponenata. Ovi pristupi uključuju inkorporaciju nanočestica srebra kao potentnog
antimikrobnog agensa (1), bioaktivnih komponenata meda (2), čestica aktivnog uglja (AU) kao nosača
terapeutski aktivnih agenasa (3), kao i primenu hidrogelova Zn-alginata koji otpuštaju jone cinka.
Dobijeni kompoziti su sveobuhvatno karakterisani u pogledu sastava, citotoksičnosti, antibakterijske
aktivnosti, kinetike otpuštanja aktivnih agenasa i tretmana rana u eksperimentalnom modelu
opekotina na pacovima.
Ag/alginatni nanokompozitni hydrogelovi su usled otpuštanja jona i/ili nanočestica srebra,
pokazali izraženu baktericidnu aktivnost prema širokom spektru standardnih i kliničkih multi-
rezistentnih bakterijskih sojeva (Escherichia coli, Staphylococcus aureus, meticilin-resistentni
Staphylococcus aureus – MRSA, Acinetobacter baumannii i Pseudomonas aeruginosa). Posebno
interesantni rezultati su dobijeni u kulturama 13 kliničkih izolata A. baumannii, gde je u 6 slučajeva
postignut potpun baktericidan efekat u toku 48 h (2). Ipak, kod 3 klinička izolata nije postignuto
antibakterijsko dejstvo što ukazuje na mogućnost razvoja bakterijske rezistencije na srebro. U
tretmanu opekotina drugog stepena na pacovima, Ag/alginatni nanokompoziti su pokazali istu
efikasnost kao komercijalni medicinski proizvodi.
Kompozitni alginatni hidrogelovi sa imobilisanim česticama AU impregniranih povidon-jodom
kao model terapeutski aktivnom komponentom, su razvijeni sa ciljem da obezbede kontrolisano
otpuštanje čestica AU u rani bez otpuštanja adsorbovane supstance kako bi se na taj način postiglo
željeno dejstvo bez neželjenih efekata sistemske apsorpcije. Kompozitni Ca-alginatni hidrogelovi su
pokazali jake baktericidne efekte na dva standardna bakterijska soja i nekoliko kliničkih multi-
rezistentnih izolata iz rana (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis i Proteus mirabilis) bez
otpuštanja povidon-joda u okolinu (3). Isto tako, kompozitni Zn-alginatni hidrogelovi su otpuštali jone
cinka uz otpuštanje AU čestica sa adsorbovanim povidon-jodom što je prouzrokovalo dodatno
mikrobicidno dejstvo na jedan divlji soj gljivice Candida albicans. Dobijeni baktericidni efekti su
pripisani efikasnoj adsorpciji bakterija na čestice AU i daljem direktnom kontaktu adsorbovanog joda
sa ćelijskom membranom bakterija, dok je antifungalna aktivnost u odnosu na C. albicans pripisana
otpuštenim Zn 2+ jonima.
Može se zaključiti da su razvijeni kompozitni alginatni hdrogelovi pokazali veliki potencijal za
primenu u raznovrsnim multifunkcionalnim oblogama za rane prilagođenim specifičnim potrebama.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Multifunctional composites based on alginate hydrogels for potential use in wound dressings
T1  - Multifunkcionalni kompoziti na bazi alginatnih hidrogelova za potencijalnu primenu u oblogama za rane
VL  - 71
IS  - 5 suplement
SP  - S22
EP  - S23
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4666
ER  - 

@conference{
author = "Obradović, Bojana and Stojkovska, Jasmina and Osmokrović, Andrea and Mišković Stanković, Vesna and Jančić, Ivan and Milenković, Marina",
year = "2021",
abstract = "Alginate hydrogels are widely used in wound dressings due to hydrophilicity, biocompatibility,
flexibility, and high sorption capacity, providing effective moisture regulation in wounds and inducing
rapid granulation and reepithelization of the damaged tissue. However, these dressings are not
bioactive so that different methodologies have been investigated to extend functionality of alginate
hydrogels.
In the present work, we show several approaches to achieve this aim by addition of different
biologically active components. These include incorporation of silver nanoparticles as potent
antimicrobial agents (1), bioactive honey components (2), activated charcoal (AC) particles as carriers
of therapeutically active agents (3) as well as the use of Zn-alginate hydrogels that release zinc ions (4).
The obtained composites were comprehensively characterized regarding composition, cytotoxicity,
antibacterial activity, release kinetics of active agents and wound treatment in a rat model.
Ag/alginate nanocomposite hydrogels releasing silver ions and/or nanoparticles exhibited high
bactericidal activity against a broad spectrum of standard and multi-drug resistant clinical bacterial
strains (Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus – MRSA,
Acinetobacter baumannii and Pseudomonas aeruginosa). Especially interesting results were obtained
against 13 clinical isolates of A. baumannii, which were completely extinguished over 48 h in 6 cases
(2). However, in 3 clinical isolates, antibacterial effects were not noticed implying possibility for
development of bacterial resistance to silver. In the treatment of 2nd degree burns in rats Ag/alginate
nanocomposites exhibited the same efficiency as commercial medical products (5).
Composite alginate hydrogels with immobilized AC particles impregnated with povidone iodine
(PVP-I) as a model therapeutically active agent, were developed with the aim to provide controlled
particle release in the wound without actually releasing the adsorbed substance, thus achieving the
desired activity without adverse effects by systemic absorption. The composite Ca-alginate hydrogels
induced strong bactericidal effects against two standard bacterial strains and clinical multi-resistant
wound isolates (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis and Proteus mirabilis) without
releasing PVP-I in the environment (3). Furthermore, composite Zn-alginate hydrogels released zinc
ions in addition to AC particles with adsorbed PVP-I, which induced additional microbicidal effects on
one wild yeast strain (Candida albicans). The obtained bactericidal effects were ascribed to effective
adsorption of bacteria onto AC particles and further direct contact with the adsorbed iodine, while the
antifungal activity against C. albicans was assigned to released Zn 2+.
Overall, the developed composite alginate hydrogels have shown high potentials for utilization
in variety of multifunctional wound dressings according to the specific needs., Alginatni hidrogelovi se široko koriste u oblogama za rane zbog svoje hidrofilnosti,
biokompatibilnosti, fleksibilnosti i velikog sorpcionog kapaciteta čime obezbeđuju efikasnu regulaciju
vlažnosti rane i podstiču brzu granulaciju i reepitelizaciju oštećenog tkiva. Međutim, ove obloge nisu
bioaktivne tako da su istraživane različite metodologije kako bi se proširila funkcionalnost alginatnih
hidrogelova.
U ovom radu je prikazano nekoliko pristupa ostvarivanju tog cilja dodatkom različitih biološki
aktivnih komponenata. Ovi pristupi uključuju inkorporaciju nanočestica srebra kao potentnog
antimikrobnog agensa (1), bioaktivnih komponenata meda (2), čestica aktivnog uglja (AU) kao nosača
terapeutski aktivnih agenasa (3), kao i primenu hidrogelova Zn-alginata koji otpuštaju jone cinka.
Dobijeni kompoziti su sveobuhvatno karakterisani u pogledu sastava, citotoksičnosti, antibakterijske
aktivnosti, kinetike otpuštanja aktivnih agenasa i tretmana rana u eksperimentalnom modelu
opekotina na pacovima.
Ag/alginatni nanokompozitni hydrogelovi su usled otpuštanja jona i/ili nanočestica srebra,
pokazali izraženu baktericidnu aktivnost prema širokom spektru standardnih i kliničkih multi-
rezistentnih bakterijskih sojeva (Escherichia coli, Staphylococcus aureus, meticilin-resistentni
Staphylococcus aureus – MRSA, Acinetobacter baumannii i Pseudomonas aeruginosa). Posebno
interesantni rezultati su dobijeni u kulturama 13 kliničkih izolata A. baumannii, gde je u 6 slučajeva
postignut potpun baktericidan efekat u toku 48 h (2). Ipak, kod 3 klinička izolata nije postignuto
antibakterijsko dejstvo što ukazuje na mogućnost razvoja bakterijske rezistencije na srebro. U
tretmanu opekotina drugog stepena na pacovima, Ag/alginatni nanokompoziti su pokazali istu
efikasnost kao komercijalni medicinski proizvodi.
Kompozitni alginatni hidrogelovi sa imobilisanim česticama AU impregniranih povidon-jodom
kao model terapeutski aktivnom komponentom, su razvijeni sa ciljem da obezbede kontrolisano
otpuštanje čestica AU u rani bez otpuštanja adsorbovane supstance kako bi se na taj način postiglo
željeno dejstvo bez neželjenih efekata sistemske apsorpcije. Kompozitni Ca-alginatni hidrogelovi su
pokazali jake baktericidne efekte na dva standardna bakterijska soja i nekoliko kliničkih multi-
rezistentnih izolata iz rana (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis i Proteus mirabilis) bez
otpuštanja povidon-joda u okolinu (3). Isto tako, kompozitni Zn-alginatni hidrogelovi su otpuštali jone
cinka uz otpuštanje AU čestica sa adsorbovanim povidon-jodom što je prouzrokovalo dodatno
mikrobicidno dejstvo na jedan divlji soj gljivice Candida albicans. Dobijeni baktericidni efekti su
pripisani efikasnoj adsorpciji bakterija na čestice AU i daljem direktnom kontaktu adsorbovanog joda
sa ćelijskom membranom bakterija, dok je antifungalna aktivnost u odnosu na C. albicans pripisana
otpuštenim Zn 2+ jonima.
Može se zaključiti da su razvijeni kompozitni alginatni hdrogelovi pokazali veliki potencijal za
primenu u raznovrsnim multifunkcionalnim oblogama za rane prilagođenim specifičnim potrebama.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Multifunctional composites based on alginate hydrogels for potential use in wound dressings, Multifunkcionalni kompoziti na bazi alginatnih hidrogelova za potencijalnu primenu u oblogama za rane",
volume = "71",
number = "5 suplement",
pages = "S22-S23",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4666"
}

Obradović, B., Stojkovska, J., Osmokrović, A., Mišković Stanković, V., Jančić, I.,& Milenković, M.. (2021). Multifunctional composites based on alginate hydrogels for potential use in wound dressings. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S22-S23.
https://hdl.handle.net/21.15107/rcub_farfar_4666

Obradović B, Stojkovska J, Osmokrović A, Mišković Stanković V, Jančić I, Milenković M. Multifunctional composites based on alginate hydrogels for potential use in wound dressings. in Arhiv za farmaciju. 2021;71(5 suplement):S22-S23.
https://hdl.handle.net/21.15107/rcub_farfar_4666 .

Obradović, Bojana, Stojkovska, Jasmina, Osmokrović, Andrea, Mišković Stanković, Vesna, Jančić, Ivan, Milenković, Marina, "Multifunctional composites based on alginate hydrogels for potential use in wound dressings" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S22-S23,
https://hdl.handle.net/21.15107/rcub_farfar_4666 .

TY  - JOUR
AU  - Đurđić, Slađana
AU  - Stanković, Vesna
AU  - Ražić, Slavica
AU  - Mutić, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3727
AB  - The analysis of isotope ratios of lead in the mushrooms and soil, where they were grown, assisted with a principal component analysis, offered a new perspective for understanding possible chemical environment in a real setup of those compartments. The content of lead and its isotope compositions were determined in soil samples and mushroom Macrolepiota procera from unpolluted area of Mountain Goč, Serbia. Sequential extraction procedure based on the Commission of the European Community Bureau of Reference (BCR) was applied on soil samples in order to determine the distribution of lead in the labile and un-labile fractions of the soil. Caps and stipes of mushrooms were subjected to microwave acid-assisted digestion prior to measurements by inductively coupled plasma quadrupole mass spectrometer for determination of lead content and lead isotope ratios. Information about the chemical fractionation of Pb in soil, Pb isotopic data from soil fractions and fruiting bodies allowed a more detailed insight on the uptake mechanisms. Lead was predominantly associated with reducible fraction (~ 60%). Only its small portion (∼ 1%) was present in the exchangeable and acid-extractable fractions suggesting the low mobility of Pb. Lead isotope analysis revealed the presence of anthropogenic lead in the surface soil. Significant lower 206Pb/207Pb compared with other fractions was found in exchangeable and acid-soluble fraction (1.331 ± 0.010), which corresponds to the isotope ratio of European gasoline. The highest 206Pb/207Pb ratio was observed in reducible fraction (1.162 ± 0.007), while in oxidizable and residual fraction, those values were similar (1.159 ± 0.006 and 1.159 ± 0.004, respectively). Distinction of exchangeable and acid-extractable fractions from others was also confirmed, for the first time, by principal component analysis. The analysis of four isotope ratios (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, and 207Pb/204Pb) indicated that the analyzed M. procera accumulates lead from the first two fractions of topsoil layers.
PB  - Springer Nature
T2  - Environmental Science and Pollution Research
T1  - Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil
DO  - 10.1007/s11356-020-07947-6
ER  - 

@article{
author = "Đurđić, Slađana and Stanković, Vesna and Ražić, Slavica and Mutić, Jelena",
year = "2020",
abstract = "The analysis of isotope ratios of lead in the mushrooms and soil, where they were grown, assisted with a principal component analysis, offered a new perspective for understanding possible chemical environment in a real setup of those compartments. The content of lead and its isotope compositions were determined in soil samples and mushroom Macrolepiota procera from unpolluted area of Mountain Goč, Serbia. Sequential extraction procedure based on the Commission of the European Community Bureau of Reference (BCR) was applied on soil samples in order to determine the distribution of lead in the labile and un-labile fractions of the soil. Caps and stipes of mushrooms were subjected to microwave acid-assisted digestion prior to measurements by inductively coupled plasma quadrupole mass spectrometer for determination of lead content and lead isotope ratios. Information about the chemical fractionation of Pb in soil, Pb isotopic data from soil fractions and fruiting bodies allowed a more detailed insight on the uptake mechanisms. Lead was predominantly associated with reducible fraction (~ 60%). Only its small portion (∼ 1%) was present in the exchangeable and acid-extractable fractions suggesting the low mobility of Pb. Lead isotope analysis revealed the presence of anthropogenic lead in the surface soil. Significant lower 206Pb/207Pb compared with other fractions was found in exchangeable and acid-soluble fraction (1.331 ± 0.010), which corresponds to the isotope ratio of European gasoline. The highest 206Pb/207Pb ratio was observed in reducible fraction (1.162 ± 0.007), while in oxidizable and residual fraction, those values were similar (1.159 ± 0.006 and 1.159 ± 0.004, respectively). Distinction of exchangeable and acid-extractable fractions from others was also confirmed, for the first time, by principal component analysis. The analysis of four isotope ratios (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, and 207Pb/204Pb) indicated that the analyzed M. procera accumulates lead from the first two fractions of topsoil layers.",
publisher = "Springer Nature",
journal = "Environmental Science and Pollution Research",
title = "Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil",
doi = "10.1007/s11356-020-07947-6"
}

Đurđić, S., Stanković, V., Ražić, S.,& Mutić, J.. (2020). Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil. in Environmental Science and Pollution Research
Springer Nature..
https://doi.org/10.1007/s11356-020-07947-6

Đurđić S, Stanković V, Ražić S, Mutić J. Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil. in Environmental Science and Pollution Research. 2020;.
doi:10.1007/s11356-020-07947-6 .

Đurđić, Slađana, Stanković, Vesna, Ražić, Slavica, Mutić, Jelena, "Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil" in Environmental Science and Pollution Research (2020),
https://doi.org/10.1007/s11356-020-07947-6 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3657
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley Periodicals, LLC.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
VL  - 108
IS  - 11
SP  - 2175
EP  - 2189
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley Periodicals, LLC.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
volume = "108",
number = "11",
pages = "2175-2189",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research - Part A
Wiley Periodicals, LLC.., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research - Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research - Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Đurđević, Željka
AU  - Jančić, Ivan
AU  - Bufan, Biljana
AU  - Milenković, Marina
AU  - Janković, Radmila
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3169
AB  - In the present study, possibilities for using novel nanocomposites based on alginate and silver nanoparticles for wound treatment were investigated in a second-degree thermal burn model in Wistar rats. Silver nanoparticles (AgNPs) were electrochemically synthesized in alginate solutions that were further utilized to obtain the Ag/alginate solution and microfibers for subsequent in vivo studies. Daily applications of the Ag/alginate colloid solution, containing AgNPs, alginate and ascorbic acid (G3), wet Ag/alginate microfibers containing AgNPs (G5) and dry Ag/alginate microfibers containing AgNPs (G6) were compared to treatments with a commercial cream containing silver sulfadiazine (G2) and a commercial Ca-alginate wound dressing containing silver ions (G4), as well as to the untreated controls (G1). Results of the in vivo study have shown faster healing in treated wounds, which completely healed on day 19 (G4, G5 and G6) and 21 (G2 and G3) after the thermal injury, while the period for complete reepitelization of untreated wounds (G1) was 25 days. The macroscopic analysis has shown that scabs fell off between day 10 and 12 after the thermal injury induction in treated groups, whereas between day 15 and 16 in the control group. These macroscopic findings were supported by the results of histopathological analyses, which have shown enhanced granulation and reepithelization, reduced inflammation and improved organization of the extracellular matrix in treated groups without adverse effects. Among the treated groups, dressings based on Ca-alginate (G4-G6) induced enhanced healing as compared to the other two groups (G2, G3), which could be attributed to additional stimuli of released Ca2+. The obtained results indicated potentials of novel nanocomposites based on alginate and AgNPs for therapeutic applications in wound treatments.
PB  - Sage Publications Ltd, London
T2  - Journal of Biomaterials Applications
T1  - Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments
VL  - 32
IS  - 9
SP  - 1197
EP  - 1211
DO  - 10.1177/0885328218759564
ER  - 

@article{
author = "Stojkovska, Jasmina and Đurđević, Željka and Jančić, Ivan and Bufan, Biljana and Milenković, Marina and Janković, Radmila and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2018",
abstract = "In the present study, possibilities for using novel nanocomposites based on alginate and silver nanoparticles for wound treatment were investigated in a second-degree thermal burn model in Wistar rats. Silver nanoparticles (AgNPs) were electrochemically synthesized in alginate solutions that were further utilized to obtain the Ag/alginate solution and microfibers for subsequent in vivo studies. Daily applications of the Ag/alginate colloid solution, containing AgNPs, alginate and ascorbic acid (G3), wet Ag/alginate microfibers containing AgNPs (G5) and dry Ag/alginate microfibers containing AgNPs (G6) were compared to treatments with a commercial cream containing silver sulfadiazine (G2) and a commercial Ca-alginate wound dressing containing silver ions (G4), as well as to the untreated controls (G1). Results of the in vivo study have shown faster healing in treated wounds, which completely healed on day 19 (G4, G5 and G6) and 21 (G2 and G3) after the thermal injury, while the period for complete reepitelization of untreated wounds (G1) was 25 days. The macroscopic analysis has shown that scabs fell off between day 10 and 12 after the thermal injury induction in treated groups, whereas between day 15 and 16 in the control group. These macroscopic findings were supported by the results of histopathological analyses, which have shown enhanced granulation and reepithelization, reduced inflammation and improved organization of the extracellular matrix in treated groups without adverse effects. Among the treated groups, dressings based on Ca-alginate (G4-G6) induced enhanced healing as compared to the other two groups (G2, G3), which could be attributed to additional stimuli of released Ca2+. The obtained results indicated potentials of novel nanocomposites based on alginate and AgNPs for therapeutic applications in wound treatments.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Biomaterials Applications",
title = "Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments",
volume = "32",
number = "9",
pages = "1197-1211",
doi = "10.1177/0885328218759564"
}

Stojkovska, J., Đurđević, Ž., Jančić, I., Bufan, B., Milenković, M., Janković, R., Mišković-Stanković, V.,& Obradović, B.. (2018). Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments. in Journal of Biomaterials Applications
Sage Publications Ltd, London., 32(9), 1197-1211.
https://doi.org/10.1177/0885328218759564

Stojkovska J, Đurđević Ž, Jančić I, Bufan B, Milenković M, Janković R, Mišković-Stanković V, Obradović B. Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments. in Journal of Biomaterials Applications. 2018;32(9):1197-1211.
doi:10.1177/0885328218759564 .

Stojkovska, Jasmina, Đurđević, Željka, Jančić, Ivan, Bufan, Biljana, Milenković, Marina, Janković, Radmila, Mišković-Stanković, Vesna, Obradović, Bojana, "Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments" in Journal of Biomaterials Applications, 32, no. 9 (2018):1197-1211,
https://doi.org/10.1177/0885328218759564 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đosić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3123
AB  - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
PB  - Amer Chemical Soc, Washington
T2  - ACS Biomaterials Science & Engineering
T1  - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
VL  - 4
IS  - 12
SP  - 3994
EP  - 4007
DO  - 10.1021/acsbiomaterials.8b00859
ER  - 

@article{
author = "Stevanović, Milena and Đosić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Biomaterials Science & Engineering",
title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium",
volume = "4",
number = "12",
pages = "3994-4007",
doi = "10.1021/acsbiomaterials.8b00859"
}

Stevanović, M., Đosić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering
Amer Chemical Soc, Washington., 4(12), 3994-4007.
https://doi.org/10.1021/acsbiomaterials.8b00859

Stevanović M, Đosić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007.
doi:10.1021/acsbiomaterials.8b00859 .

Stevanović, Milena, Đosić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007,
https://doi.org/10.1021/acsbiomaterials.8b00859 . .

TY  - CONF
AU  - Zvicer, Jovana S
AU  - Girandon, L.
AU  - Potocar, U.
AU  - Froehlich, M.
AU  - Jančić, Ivan
AU  - Bufan, Biljana
AU  - Milenković, Marina
AU  - Stojkovska, J.
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2220
PB  - Wiley-Blackwell, Hoboken
C3  - Journal of Tissue Engineering and Regenerative Medicine
T1  - Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment
VL  - 8
IS  - Supplement 1
SP  - 345
EP  - 345
DO  - 10.1002/term.1932
ER  - 

@conference{
author = "Zvicer, Jovana S and Girandon, L. and Potocar, U. and Froehlich, M. and Jančić, Ivan and Bufan, Biljana and Milenković, Marina and Stojkovska, J. and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
title = "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment",
volume = "8",
number = "Supplement 1",
pages = "345-345",
doi = "10.1002/term.1932"
}

Zvicer, J. S., Girandon, L., Potocar, U., Froehlich, M., Jančić, I., Bufan, B., Milenković, M., Stojkovska, J., Mišković-Stanković, V.,& Obradović, B.. (2014). Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine
Wiley-Blackwell, Hoboken., 8(Supplement 1), 345-345.
https://doi.org/10.1002/term.1932

Zvicer JS, Girandon L, Potocar U, Froehlich M, Jančić I, Bufan B, Milenković M, Stojkovska J, Mišković-Stanković V, Obradović B. Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine. 2014;8(Supplement 1):345-345.
doi:10.1002/term.1932 .

Zvicer, Jovana S, Girandon, L., Potocar, U., Froehlich, M., Jančić, Ivan, Bufan, Biljana, Milenković, Marina, Stojkovska, J., Mišković-Stanković, Vesna, Obradović, Bojana, "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment" in Journal of Tissue Engineering and Regenerative Medicine, 8, no. Supplement 1 (2014):345-345,
https://doi.org/10.1002/term.1932 . .