TY  - JOUR
AU  - Vučković, Nemanja
AU  - Prlainović, Nevena
AU  - Glođović, Nikola
AU  - Čalija, Bojan
AU  - Milosavljević, Nedeljko
AU  - Kalagasidis Krušić, Melina
AU  - Milašinović, Nikola
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5504
AB  - Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.
T2  - Journal of the Iranian Chemical Society
T1  - Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system
VL  - 21
IS  - 3
SP  - 781
EP  - 792
DO  - 10.1007/s13738-023-02960-1
ER  - 

@article{
author = "Vučković, Nemanja and Prlainović, Nevena and Glođović, Nikola and Čalija, Bojan and Milosavljević, Nedeljko and Kalagasidis Krušić, Melina and Milašinović, Nikola",
year = "2024",
abstract = "Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.",
journal = "Journal of the Iranian Chemical Society",
title = "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system",
volume = "21",
number = "3",
pages = "781-792",
doi = "10.1007/s13738-023-02960-1"
}

Vučković, N., Prlainović, N., Glođović, N., Čalija, B., Milosavljević, N., Kalagasidis Krušić, M.,& Milašinović, N.. (2024). Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society, 21(3), 781-792.
https://doi.org/10.1007/s13738-023-02960-1

Vučković N, Prlainović N, Glođović N, Čalija B, Milosavljević N, Kalagasidis Krušić M, Milašinović N. Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society. 2024;21(3):781-792.
doi:10.1007/s13738-023-02960-1 .

Vučković, Nemanja, Prlainović, Nevena, Glođović, Nikola, Čalija, Bojan, Milosavljević, Nedeljko, Kalagasidis Krušić, Melina, Milašinović, Nikola, "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system" in Journal of the Iranian Chemical Society, 21, no. 3 (2024):781-792,
https://doi.org/10.1007/s13738-023-02960-1 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Kotur-Stevuljević, Jelena
AU  - Dilber, Sanda
AU  - Kalagasidis-Krusić, Melina T.
AU  - Đorđević, Brižita
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2605
AB  - Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity
VL  - 70
IS  - 1
SP  - 49
EP  - 58
DO  - 10.2298/HEMIND141119010V
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Kotur-Stevuljević, Jelena and Dilber, Sanda and Kalagasidis-Krusić, Melina T. and Đorđević, Brižita and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity",
volume = "70",
number = "1",
pages = "49-58",
doi = "10.2298/HEMIND141119010V"
}

Vidović, B., Milašinović, N., Kotur-Stevuljević, J., Dilber, S., Kalagasidis-Krusić, M. T., Đorđević, B.,& Knežević-Jugović, Z.. (2016). Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(1), 49-58.
https://doi.org/10.2298/HEMIND141119010V

Vidović B, Milašinović N, Kotur-Stevuljević J, Dilber S, Kalagasidis-Krusić MT, Đorđević B, Knežević-Jugović Z. Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija. 2016;70(1):49-58.
doi:10.2298/HEMIND141119010V .

Vidović, Bojana, Milašinović, Nikola, Kotur-Stevuljević, Jelena, Dilber, Sanda, Kalagasidis-Krusić, Melina T., Đorđević, Brižita, Knežević-Jugović, Zorica, "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity" in Hemijska industrija, 70, no. 1 (2016):49-58,
https://doi.org/10.2298/HEMIND141119010V . .

TY  - CONF
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Vidović, Jana
AU  - Čalija, Bojan
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Milić, Jela
AU  - Đorđević, Brižita
AU  - Kalagasidis-Krusić, Melina T.
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2333
AB  - Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.
PB  - Karger, Basel
C3  - Annals of Nutrition and Metabolism
T1  - Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity
VL  - 67
IS  - Supplement 1
SP  - 484
EP  - 485
DO  - 10.1159/000440895
ER  - 

@conference{
author = "Vidović, Bojana and Milašinović, Nikola and Vidović, Jana and Čalija, Bojan and Crevar-Sakač, Milkica and Vujić, Zorica and Milić, Jela and Đorđević, Brižita and Kalagasidis-Krusić, Melina T. and Knežević-Jugović, Zorica",
year = "2015",
abstract = "Introduction: Αlpha-Lipoic acid (LA) has gained considerable
attention as a nutraceutical agent due to its various medicinal proper-
ties. Despite its safety and effectiveness LA utilization is limited by its
low bioavailability and stability. Recently, there has been an increasing
interest in the developing of efficient oral delivery systems, such as
LA/chitosan conjugate, for protection and controlled release of LA to
enhance its oral bioavailability with improved biological potential.
Objectives: The aim of the research was to explore, both in vitro
antioxidative activity of LA upon encapsulation into chitosan micro-
particles (LA/chitosan conjugate formation) and its release.
Method / Design: LA was encapsulated by dip-coating method
onto ready-made chitosan microparticles of predetermined particle
size, prepared by reverse emulsion polymerization technique and
the encapsulation efficiency was determined, as well. Structural
interactions of LA with chitosan within the conjugate were revealed
by Fourier Transform Infrared (FT-IR) spectroscopy and Differental
Scanning Calorimetry (DSC). Also, the prepared LA/chitosan conjugates were evaluated for in vitro released LA antioxidative activity.
Results: The applied technique allowed the production of chitosan microparticles with an average diameter between 135 μm and 169
μm, in its dried state. Furthermore, the encapsulation efficiency of LA 12th European Nutrition Conference 2015Ann Nutr Metab 2015; 67(suppl 1) 485
was up to 50%. FT-IR analyses confirmed the presence of LA within
synthetized microparticles. The disappearance of melting peak of pure
LA upon encapsulation, observed at DSC thermogram, could be ascribed to the formation of LA/chitosan conjugate. A satisfactory level of
antioxidative activity after sustained release of LA in pH 6.8 has been
confirmed by FRAP (showing up to 56 μmolFe(II)/gmicroparticles)
and ABTS (showing up to 85 μmolTrolox/gmicroparticles) assays.
Conclusions: The results showed that the prepared LA/chitosan
microparticles conjugate could be used for encapsulation of LA and
exhibited the potential of preserving its activity for a longer period of
time, by improving its stability and functionality.",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity",
volume = "67",
number = "Supplement 1",
pages = "484-485",
doi = "10.1159/000440895"
}

Vidović, B., Milašinović, N., Vidović, J., Čalija, B., Crevar-Sakač, M., Vujić, Z., Milić, J., Đorđević, B., Kalagasidis-Krusić, M. T.,& Knežević-Jugović, Z.. (2015). Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism
Karger, Basel., 67(Supplement 1), 484-485.
https://doi.org/10.1159/000440895

Vidović B, Milašinović N, Vidović J, Čalija B, Crevar-Sakač M, Vujić Z, Milić J, Đorđević B, Kalagasidis-Krusić MT, Knežević-Jugović Z. Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity. in Annals of Nutrition and Metabolism. 2015;67(Supplement 1):484-485.
doi:10.1159/000440895 .

Vidović, Bojana, Milašinović, Nikola, Vidović, Jana, Čalija, Bojan, Crevar-Sakač, Milkica, Vujić, Zorica, Milić, Jela, Đorđević, Brižita, Kalagasidis-Krusić, Melina T., Knežević-Jugović, Zorica, "Preparation of α-lipoic acid/chitosan microparticle conjugate and its in vitro
antioxidative activity" in Annals of Nutrition and Metabolism, 67, no. Supplement 1 (2015):484-485,
https://doi.org/10.1159/000440895 . .