TY  - JOUR
AU  - Pantić Biševac, Jelena
AU  - Đukić, Mirjana
AU  - Stanojević, Ivan
AU  - Stevanović, Ivana
AU  - Mijušković, Željko
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Banović, Tatjana
AU  - Vojvodić, Danilo
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3206
AB  - Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Association between oxidative stress and melanoma progression
VL  - 37
IS  - 1
SP  - 12
EP  - 20
DO  - 10.1515/jomb-2017-0040
ER  - 

@article{
author = "Pantić Biševac, Jelena and Đukić, Mirjana and Stanojević, Ivan and Stevanović, Ivana and Mijušković, Željko and Đurić, Ana and Gobeljić, Borko and Banović, Tatjana and Vojvodić, Danilo",
year = "2018",
abstract = "Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Association between oxidative stress and melanoma progression",
volume = "37",
number = "1",
pages = "12-20",
doi = "10.1515/jomb-2017-0040"
}

Pantić Biševac, J., Đukić, M., Stanojević, I., Stevanović, I., Mijušković, Ž., Đurić, A., Gobeljić, B., Banović, T.,& Vojvodić, D.. (2018). Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 37(1), 12-20.
https://doi.org/10.1515/jomb-2017-0040

Pantić Biševac J, Đukić M, Stanojević I, Stevanović I, Mijušković Ž, Đurić A, Gobeljić B, Banović T, Vojvodić D. Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry. 2018;37(1):12-20.
doi:10.1515/jomb-2017-0040 .

Pantić Biševac, Jelena, Đukić, Mirjana, Stanojević, Ivan, Stevanović, Ivana, Mijušković, Željko, Đurić, Ana, Gobeljić, Borko, Banović, Tatjana, Vojvodić, Danilo, "Association between oxidative stress and melanoma progression" in Journal of Medical Biochemistry, 37, no. 1 (2018):12-20,
https://doi.org/10.1515/jomb-2017-0040 . .

TY  - JOUR
AU  - Stanojević, Ivan
AU  - Miller, Karolina
AU  - Kandolf-Sekulović, Lidija
AU  - Mijušković, Željko
AU  - Zolotarevska, Lidija
AU  - Jović, Milena
AU  - Gacević, Milomir
AU  - Đukić, Mirjana
AU  - Arsenijević, Nebojša
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2565
AB  - Seventy-eight melanoma patients and 10 healthy individuals were examined. Follow-up examinations of all melanoma patients were performed regularly every three months. Myeloid-derived suppressor cells (MDSC) were defined as lineage negative (CD3(-), CD19(-), CD56(-)), HLA-DR-/low, CD11b(+) and CD33(+). Classification of granulocytic (GrMDSC) and monocytic (MoMDSC) subsets was based on the CD15 and CD14 expression, respectively. Unlike the MoMDSC, that were present in 60% of healthy controls and 15% of melanoma patients, the GrMDSC were present in all examined participants, and the melanoma patients were found to have statistically higher frequencies compared with healthy controls. Accordingly, we kept focused on GrMDSC frequencies in relation to the melanoma stages and course of the disease. The GrMDSC values are highest in stage IV melanoma patients, with statistical significance compared with stages IA, IB, IIA and IIB. Patients with progression had statistically higher GrMDSC counts comparing with those with stable disease (P = 0.0079). Patients who had progression-free interval (PFI)  lt  12 months showed significantly higher GrMDSC values compared with those with PFI > 12 months (P = 0.0333). GrMDSC showed significant negative correlation with PFI intervals (P = 0.0095). The GrMDSC subset was predominant in all our patients. We confirmed that GrMDSC do accumulate early in the peripheral blood of melanoma patients and their frequencies correlate narrowly with the clinical stage and the spread of the disease. The increase in GrMDSC frequencies correlates well with a progressive disease and could be considered a potential predictive biomarker of high-risk melanoma cases that are more likely to have a shorter PFI.
PB  - Oxford Univ Press, Oxford
T2  - International Immunology
T1  - A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma
VL  - 28
IS  - 2
SP  - 87
EP  - 97
DO  - 10.1093/intimm/dxv053
ER  - 

@article{
author = "Stanojević, Ivan and Miller, Karolina and Kandolf-Sekulović, Lidija and Mijušković, Željko and Zolotarevska, Lidija and Jović, Milena and Gacević, Milomir and Đukić, Mirjana and Arsenijević, Nebojša and Vojvodić, Danilo",
year = "2016",
abstract = "Seventy-eight melanoma patients and 10 healthy individuals were examined. Follow-up examinations of all melanoma patients were performed regularly every three months. Myeloid-derived suppressor cells (MDSC) were defined as lineage negative (CD3(-), CD19(-), CD56(-)), HLA-DR-/low, CD11b(+) and CD33(+). Classification of granulocytic (GrMDSC) and monocytic (MoMDSC) subsets was based on the CD15 and CD14 expression, respectively. Unlike the MoMDSC, that were present in 60% of healthy controls and 15% of melanoma patients, the GrMDSC were present in all examined participants, and the melanoma patients were found to have statistically higher frequencies compared with healthy controls. Accordingly, we kept focused on GrMDSC frequencies in relation to the melanoma stages and course of the disease. The GrMDSC values are highest in stage IV melanoma patients, with statistical significance compared with stages IA, IB, IIA and IIB. Patients with progression had statistically higher GrMDSC counts comparing with those with stable disease (P = 0.0079). Patients who had progression-free interval (PFI)  lt  12 months showed significantly higher GrMDSC values compared with those with PFI > 12 months (P = 0.0333). GrMDSC showed significant negative correlation with PFI intervals (P = 0.0095). The GrMDSC subset was predominant in all our patients. We confirmed that GrMDSC do accumulate early in the peripheral blood of melanoma patients and their frequencies correlate narrowly with the clinical stage and the spread of the disease. The increase in GrMDSC frequencies correlates well with a progressive disease and could be considered a potential predictive biomarker of high-risk melanoma cases that are more likely to have a shorter PFI.",
publisher = "Oxford Univ Press, Oxford",
journal = "International Immunology",
title = "A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma",
volume = "28",
number = "2",
pages = "87-97",
doi = "10.1093/intimm/dxv053"
}

Stanojević, I., Miller, K., Kandolf-Sekulović, L., Mijušković, Ž., Zolotarevska, L., Jović, M., Gacević, M., Đukić, M., Arsenijević, N.,& Vojvodić, D.. (2016). A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma. in International Immunology
Oxford Univ Press, Oxford., 28(2), 87-97.
https://doi.org/10.1093/intimm/dxv053

Stanojević I, Miller K, Kandolf-Sekulović L, Mijušković Ž, Zolotarevska L, Jović M, Gacević M, Đukić M, Arsenijević N, Vojvodić D. A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma. in International Immunology. 2016;28(2):87-97.
doi:10.1093/intimm/dxv053 .

Stanojević, Ivan, Miller, Karolina, Kandolf-Sekulović, Lidija, Mijušković, Željko, Zolotarevska, Lidija, Jović, Milena, Gacević, Milomir, Đukić, Mirjana, Arsenijević, Nebojša, Vojvodić, Danilo, "A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma" in International Immunology, 28, no. 2 (2016):87-97,
https://doi.org/10.1093/intimm/dxv053 . .

TY  - JOUR
AU  - Bisevac, Jelena Pantic
AU  - Stanojević, Ivan
AU  - Mijušković, Željko
AU  - Banović, Tatjana
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2754
AB  - Background: The immune response in patients with melanoma is an important focus of research due to the tumor's resistance and immunotherapy possibilities. IL-27 is one of the cytokines with antitumor properties. The role of IL-27 in the pathogenesis of melanoma is still unclear. The aim of this study was to examine the association between serum IL-27 levels and the clinical parameters of melanoma patients. Methods: The IL-27 concentration was determined by commercial ELISA in serum samples from melanoma patients (n=72) and healthy control subjects (n=44). Patients were classified according to AJCC clinical stage, TNM stage, the length of progression-free interval (PFI) and the extent of the disease (localized or widespread). Results: Average IL-27 values were increased in patients with early stages of melanoma compared to patients with terminal stages and control values. The highest IL-27 concentration was found in stage Ila. Patients in stages III and IV had significantly lower values of IL-27 compared to control. Patients with localized melanoma and shorter PFI had insignificantly increased IL-27 levels compared to patients with widespread disease and longer PFI. Patients with metastatic disease and stage TNM4 had significantly lower average IL-27 values compared to control. Patients with high production of IL-27 (>1000 pg/mL) were most numerous in Ila AJCC stage, with initial tumor size TNM2 and in the group of patients with localized disease. Conclusions: High levels of IL-27 in patients with melanoma are associated with the initial stages and localized disease.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - High Interleukin 27 Production is Associated with Early Clinical Stage and Localized Disease in Patients with Melanoma
VL  - 35
IS  - 4
SP  - 443
EP  - 450
DO  - 10.1515/jomb-2016-0018
ER  - 

@article{
author = "Bisevac, Jelena Pantic and Stanojević, Ivan and Mijušković, Željko and Banović, Tatjana and Đukić, Mirjana and Vojvodić, Danilo",
year = "2016",
abstract = "Background: The immune response in patients with melanoma is an important focus of research due to the tumor's resistance and immunotherapy possibilities. IL-27 is one of the cytokines with antitumor properties. The role of IL-27 in the pathogenesis of melanoma is still unclear. The aim of this study was to examine the association between serum IL-27 levels and the clinical parameters of melanoma patients. Methods: The IL-27 concentration was determined by commercial ELISA in serum samples from melanoma patients (n=72) and healthy control subjects (n=44). Patients were classified according to AJCC clinical stage, TNM stage, the length of progression-free interval (PFI) and the extent of the disease (localized or widespread). Results: Average IL-27 values were increased in patients with early stages of melanoma compared to patients with terminal stages and control values. The highest IL-27 concentration was found in stage Ila. Patients in stages III and IV had significantly lower values of IL-27 compared to control. Patients with localized melanoma and shorter PFI had insignificantly increased IL-27 levels compared to patients with widespread disease and longer PFI. Patients with metastatic disease and stage TNM4 had significantly lower average IL-27 values compared to control. Patients with high production of IL-27 (>1000 pg/mL) were most numerous in Ila AJCC stage, with initial tumor size TNM2 and in the group of patients with localized disease. Conclusions: High levels of IL-27 in patients with melanoma are associated with the initial stages and localized disease.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "High Interleukin 27 Production is Associated with Early Clinical Stage and Localized Disease in Patients with Melanoma",
volume = "35",
number = "4",
pages = "443-450",
doi = "10.1515/jomb-2016-0018"
}

Bisevac, J. P., Stanojević, I., Mijušković, Ž., Banović, T., Đukić, M.,& Vojvodić, D.. (2016). High Interleukin 27 Production is Associated with Early Clinical Stage and Localized Disease in Patients with Melanoma. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(4), 443-450.
https://doi.org/10.1515/jomb-2016-0018

Bisevac JP, Stanojević I, Mijušković Ž, Banović T, Đukić M, Vojvodić D. High Interleukin 27 Production is Associated with Early Clinical Stage and Localized Disease in Patients with Melanoma. in Journal of Medical Biochemistry. 2016;35(4):443-450.
doi:10.1515/jomb-2016-0018 .

Bisevac, Jelena Pantic, Stanojević, Ivan, Mijušković, Željko, Banović, Tatjana, Đukić, Mirjana, Vojvodić, Danilo, "High Interleukin 27 Production is Associated with Early Clinical Stage and Localized Disease in Patients with Melanoma" in Journal of Medical Biochemistry, 35, no. 4 (2016):443-450,
https://doi.org/10.1515/jomb-2016-0018 . .