TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - CONF
AU  - Radović-Selgrad, Jelena
AU  - Milutinović, Violeta
AU  - Dragićević-Sudimac, Katarina
AU  - Vićentijević, Lazar
AU  - Kopanja, Đurđica
AU  - Kundaković-Vasović, Tatjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5149
AB  - Sorbus torminalis (L.) Crantz (Rosaceae), брекиња, је листопадно дрво средње величине које потиче из централне и јужне Европе, северозападне Африке, Балканског полуострва и Мале Азије. Разни делови биљке, укључујући плод, лист, цваст и кору брекиње, традиционално се користе као диуретици, антид ијароици, антиинфламатори, антидијабетици и антиатерогена средства, вазопротективи и вазорелаксанси, али и као добри антиоксиданси. ...
AB  - Sorbus torminalis (L.) Crantz (Rosaceae) is a medium-sized deciduous tree native to central and southern Europe, northwestern Africa, the Balkan Peninsula, and western Asia. Various parts of the plant, including the fruit, leaf, inflorescence, and bark are traditionally used as diuretics, antidiarrheals, anti-inflammatory, antidiabetic and antiatherogenic agents, vasoprotective and vasorelaxants, but also as potent antioxidants. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz
T1  - Enzyme inhibitory properties of the methanol extract of the tree bark of Sorbus torminalis (L.) Crantz
SP  - 39
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5149
ER  - 

@conference{
author = "Radović-Selgrad, Jelena and Milutinović, Violeta and Dragićević-Sudimac, Katarina and Vićentijević, Lazar and Kopanja, Đurđica and Kundaković-Vasović, Tatjana",
year = "2023",
abstract = "Sorbus torminalis (L.) Crantz (Rosaceae), брекиња, је листопадно дрво средње величине које потиче из централне и јужне Европе, северозападне Африке, Балканског полуострва и Мале Азије. Разни делови биљке, укључујући плод, лист, цваст и кору брекиње, традиционално се користе као диуретици, антид ијароици, антиинфламатори, антидијабетици и антиатерогена средства, вазопротективи и вазорелаксанси, али и као добри антиоксиданси. ..., Sorbus torminalis (L.) Crantz (Rosaceae) is a medium-sized deciduous tree native to central and southern Europe, northwestern Africa, the Balkan Peninsula, and western Asia. Various parts of the plant, including the fruit, leaf, inflorescence, and bark are traditionally used as diuretics, antidiarrheals, anti-inflammatory, antidiabetic and antiatherogenic agents, vasoprotective and vasorelaxants, but also as potent antioxidants. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz, Enzyme inhibitory properties of the methanol extract of the tree bark of Sorbus torminalis (L.) Crantz",
pages = "39-41",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5149"
}

Radović-Selgrad, J., Milutinović, V., Dragićević-Sudimac, K., Vićentijević, L., Kopanja, Đ.,& Kundaković-Vasović, T.. (2023). Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 39-41.
https://hdl.handle.net/21.15107/rcub_farfar_5149

Radović-Selgrad J, Milutinović V, Dragićević-Sudimac K, Vićentijević L, Kopanja Đ, Kundaković-Vasović T. Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:39-41.
https://hdl.handle.net/21.15107/rcub_farfar_5149 .

Radović-Selgrad, Jelena, Milutinović, Violeta, Dragićević-Sudimac, Katarina, Vićentijević, Lazar, Kopanja, Đurđica, Kundaković-Vasović, Tatjana, "Ензимска инхибиторна својства метанолног екстракта коре стабла брекиње, Sorbus torminalis (L.) Crantz" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):39-41,
https://hdl.handle.net/21.15107/rcub_farfar_5149 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5267
AB  - Биљке рода Micromeria Benth. (Lamiaceae) традиционално се користе код срчаних
тегоба, респираторних болести (астме), главобоље, прехладе, у третману рана и инфекција
коже. Неке од њих су испитиване у погледу композиције секундарних метаболита, као и
фармаколошких активности. ...
AB  - Species belonging to the genus Micromeria Benth. (Lamiaceae) are traditionally used in the
treatment of heart problems, respiratory diseases (asthma), headaches, cold, wounds and skin
infections. For some of them the composition of secondary metabolites, as well as pharmacological
activities were investigated. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae)
T1  - Chemical composition of essential oil and hydroethanol extract of herb of Micromeria nervosa (Desf.) Benth. (Lamiaceae)
SP  - 21
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5267
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Биљке рода Micromeria Benth. (Lamiaceae) традиционално се користе код срчаних
тегоба, респираторних болести (астме), главобоље, прехладе, у третману рана и инфекција
коже. Неке од њих су испитиване у погледу композиције секундарних метаболита, као и
фармаколошких активности. ..., Species belonging to the genus Micromeria Benth. (Lamiaceae) are traditionally used in the
treatment of heart problems, respiratory diseases (asthma), headaches, cold, wounds and skin
infections. For some of them the composition of secondary metabolites, as well as pharmacological
activities were investigated. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae), Chemical composition of essential oil and hydroethanol extract of herb of Micromeria nervosa (Desf.) Benth. (Lamiaceae)",
pages = "21-22",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5267"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 21-22.
https://hdl.handle.net/21.15107/rcub_farfar_5267

Milutinović V, Ušjak L, Niketić M, Petrović S. Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:21-22.
https://hdl.handle.net/21.15107/rcub_farfar_5267 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae)" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):21-22,
https://hdl.handle.net/21.15107/rcub_farfar_5267 .

TY  - CONF
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Rajab Elferjane, Rajab Elferjane
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5261
AB  - Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.
PB  - University of East Sarajevo, Faculty of technology
C3  - VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
T1  - Maceration and heat-assisted extraction of polyphenols from Aloe vera
SP  - 136
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5261
ER  - 

@conference{
author = "Čutović, Natalija and Jovanović, Aleksandra and Rajab Elferjane, Rajab Elferjane and Milutinović, Violeta and Petrović, Predrag and Marinković, Aleksandar and Bugarski, Branko",
year = "2023",
abstract = "Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, 
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, 
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, 
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal 
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used 
extraction procedures because of their simple operation and low costs. In the present study, A. 
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The 
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and 
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content 
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the 
most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally 
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and 
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant 
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min 
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the 
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg 
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different 
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol 
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different 
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract 
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration 
when also reached the steady state (~40.4 mg/mL), but there were no significant differences 
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the 
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained 
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and 
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and 
cosmetics.",
publisher = "University of East Sarajevo, Faculty of technology",
journal = "VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings",
title = "Maceration and heat-assisted extraction of polyphenols from Aloe vera",
pages = "136-143",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5261"
}

Čutović, N., Jovanović, A., Rajab Elferjane, R. E., Milutinović, V., Petrović, P., Marinković, A.,& Bugarski, B.. (2023). Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings
University of East Sarajevo, Faculty of technology., 136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261

Čutović N, Jovanović A, Rajab Elferjane RE, Milutinović V, Petrović P, Marinković A, Bugarski B. Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings. 2023;:136-143.
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

Čutović, Natalija, Jovanović, Aleksandra, Rajab Elferjane, Rajab Elferjane, Milutinović, Violeta, Petrović, Predrag, Marinković, Aleksandar, Bugarski, Branko, "Maceration and heat-assisted extraction of polyphenols from Aloe vera" in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings (2023):136-143,
https://hdl.handle.net/21.15107/rcub_farfar_5261 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Petković, Miloš
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5180
AB  - The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites
VL  - 20
IS  - 11
SP  - e202301044
DO  - 10.1002/cbdv.202301044
ER  - 

@article{
author = "Milutinović, Violeta and Petrović, Predrag and Petković, Miloš and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites",
volume = "20",
number = "11",
pages = "e202301044",
doi = "10.1002/cbdv.202301044"
}

Milutinović, V., Petrović, P., Petković, M., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity
John Wiley and Sons Inc., 20(11), e202301044.
https://doi.org/10.1002/cbdv.202301044

Milutinović V, Petrović P, Petković M, Klaus A, Ušjak L, Niketić M, Petrović S. Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity. 2023;20(11):e202301044.
doi:10.1002/cbdv.202301044 .

Milutinović, Violeta, Petrović, Predrag, Petković, Miloš, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites" in Chemistry and Biodiversity, 20, no. 11 (2023):e202301044,
https://doi.org/10.1002/cbdv.202301044 . .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5086
AB  - Stachys subcrenata Vis. (Lamiaceae) је вишегодишња зељаста биљка која припада комплексној групи S. recta и данас се углавном сматра њеном подврстом. Описана је из Далмације, и до сада је са сигурношћу позната за приобаље и залеђе Јадранског мора, иако се наводи и за источни и јужни Балкан, Анадолију и Иран. ...
AB  - Stachys subcrenata Vis. (Lamiaceae) is a perennial herb that belongs to the complex group S. recta and nowadays it is mostly considered аs its subspecies. It has been described from Dalmatia, and so far it is known with certainty for the coast and hinterland of the Adriatic Sea, although it was also reported for the eastern and southern Balkans, Anatolia and Iran. Plant inhabits stony and rocky places, often by the sea coast. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Нови Сад
C3  - 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака
T1  - Фенолни састојци метанолног екстракта хербе Stachys subcrenata
T1  - Phenolic constituents of Stachys subcrenata herb methanol extract
SP  - 24
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5086
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Stachys subcrenata Vis. (Lamiaceae) је вишегодишња зељаста биљка која припада комплексној групи S. recta и данас се углавном сматра њеном подврстом. Описана је из Далмације, и до сада је са сигурношћу позната за приобаље и залеђе Јадранског мора, иако се наводи и за источни и јужни Балкан, Анадолију и Иран. ..., Stachys subcrenata Vis. (Lamiaceae) is a perennial herb that belongs to the complex group S. recta and nowadays it is mostly considered аs its subspecies. It has been described from Dalmatia, and so far it is known with certainty for the coast and hinterland of the Adriatic Sea, although it was also reported for the eastern and southern Balkans, Anatolia and Iran. Plant inhabits stony and rocky places, often by the sea coast. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Нови Сад",
journal = "2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака",
title = "Фенолни састојци метанолног екстракта хербе Stachys subcrenata, Phenolic constituents of Stachys subcrenata herb methanol extract",
pages = "24-26",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5086"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2023). Фенолни састојци метанолног екстракта хербе Stachys subcrenata. in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 24-26.
https://hdl.handle.net/21.15107/rcub_farfar_5086

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Фенолни састојци метанолног екстракта хербе Stachys subcrenata. in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака. 2023;:24-26.
https://hdl.handle.net/21.15107/rcub_farfar_5086 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Фенолни састојци метанолног екстракта хербе Stachys subcrenata" in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака (2023):24-26,
https://hdl.handle.net/21.15107/rcub_farfar_5086 .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4988
AB  - The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists.
In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).
C3  - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts
T1  - Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract
SP  - 196
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4988
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists.
In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).",
journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts",
title = "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract",
pages = "196-196",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4988"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2023). Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 196-196.
https://hdl.handle.net/21.15107/rcub_farfar_4988

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:196-196.
https://hdl.handle.net/21.15107/rcub_farfar_4988 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):196-196,
https://hdl.handle.net/21.15107/rcub_farfar_4988 .

TY  - JOUR
AU  - Rajab Elferjane, Muna
AU  - Jovanović, Aleksandra A.
AU  - Milutinović, Violeta
AU  - Čutović, Natalija
AU  - Jovanović Krivokuća, Milica
AU  - Marinković, Aleksandar
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4956
AB  - In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.
PB  - MDPI
T2  - Plants
T1  - From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities
VL  - 12
IS  - 14
DO  - 10.3390/plants12142744
ER  - 

@article{
author = "Rajab Elferjane, Muna and Jovanović, Aleksandra A. and Milutinović, Violeta and Čutović, Natalija and Jovanović Krivokuća, Milica and Marinković, Aleksandar",
year = "2023",
abstract = "In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.",
publisher = "MDPI",
journal = "Plants",
title = "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities",
volume = "12",
number = "14",
doi = "10.3390/plants12142744"
}

Rajab Elferjane, M., Jovanović, A. A., Milutinović, V., Čutović, N., Jovanović Krivokuća, M.,& Marinković, A.. (2023). From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants
MDPI., 12(14).
https://doi.org/10.3390/plants12142744

Rajab Elferjane M, Jovanović AA, Milutinović V, Čutović N, Jovanović Krivokuća M, Marinković A. From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants. 2023;12(14).
doi:10.3390/plants12142744 .

Rajab Elferjane, Muna, Jovanović, Aleksandra A., Milutinović, Violeta, Čutović, Natalija, Jovanović Krivokuća, Milica, Marinković, Aleksandar, "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities" in Plants, 12, no. 14 (2023),
https://doi.org/10.3390/plants12142744 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4585
AB  - Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) is a dwarfish shrub,
which range includes eastern part of the Balkan Peninsula (Albania, Bulgaria, Serbia) (1).
The aim of the investigation was to chemically characterize aerial flowering parts of this
plant: the dried methanol extract (TM), its alkaloid (AF) and non-alkaloid (NAF) fractions,
and to assess their ability and the ability of detected flavonoids to inhibit enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), in vitro in the Ellman
spectrophotometric test. Plant material was collected in eastern Serbia (Jelašnička Klisura
gorge), dried, powdered and extracted with methanol, after dichloromethane pre-extraction.
Using solvents of different polarities and pH value, AF (yield 14.35%) and NAF (yield
57.78%) were obtained from TM. By LC-MS analysis, flavonoids luteolin-8-C-
glucosylpentoside (159.81 mg/g), genistin (22.38 mg/g), vitexin (6.32 mg/g), rutin (4.25
mg/g), and genistein (0.86 mg/g) were detected and quantified in TM, while luteolin-8-C-
glucosylpentoside (212.70 mg/g), rutin (1.38 mg/g), and genistin (1.03 mg/g) in NAF. Using
GC-FID-MS analysis, quinolizidine alkaloids sparteine, 17-oksosparteine, and lupanine were
identified in AF. TM and NAF exhibited significant and comparable anti-cholinesterase
activity (IC50AChE 0.54 and 0.72 mg/mL; IC50BuChE 0.45 and 0.68 mg/mL). All detected
flavonoids evinced the ability to inhibit enzymes; the most active were genistein and vitexin
(IC50AChE 54.75 and 88.35 μg/mL; IC 50BuChE 27.83 and 49.77 μg/mL). Considering the
quinolizidine alkaloids toxicity and AF poor anti-cholinesterase activity, it might be
concluded, regarding safety profile and medical potential, that the fraction of the herb
methanol extract, flavonoid-rich and alkaloid-free (NAF), is good candidate for further
research.
AB  - Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) je polužbun, koji
raste na području istočnog dela Balkanskog poluostrva (Albanija, Bugarska, Srbija) (1). Cilj
istraživanja bio je da se izvrši hemijska karakterizacija nadzemnih delova u cvetu ove biljke:
suvog metanolnog ekstrakta (TM), njegove alkaloidne (AF) i frakcije bez alkaloida (NAF), i da
se in vitro, u Ellmanov-om spektrofotomerijskom testu, ispita njihova sposobnost, kao i
sposobnost detektovanih flavonoida da inhibiraju enzime acetilholinesterazu (AChE) i
butirilholinesterazu (BChE). Biljni materijal je sakupljen u istočnoj Srbiji (Jelašnička klisura),
osušen, samleven i ekstrahovan metanolom, nakon pre-ekstrakcije dihlormetanom.
Primenom rastvarača različite polarnosti uz promenu pH vrednosti, iz TM su dobijene AF
(prinos 14,35%) i NAF (prinos 57,78%). LC-MS analizom, u TM detektovani su i
kvanitikovani flavonoidi luteolin-8-C-glukozilpentozid (159,81 mg/g), genistin (22,38 mg/g),
viteksin (6,32 mg/g) i rutin (4,25 mg/g) i genistein (0,86 mg/g), a u NAF luteolin-8-C-
glukozilpentozid (212,70 mg/g), rutin (1,38 mg/g) i genistin (1,03 mg/g). GC-FID-MS
analizom, u AF identifikovani su hinolizidinski alkaloidi spartein, 17-oksospartein i lupanin.
TM i NAF ispoljili su značajnu i uporedivu antiholinesteraznu aktivnost (IC50AChE 0,54 i 0,72
mg/mL; IC 50BChE 0,45 i 0,68 mg/mL). Svi detektovani flavonoidi su pokazali sposobnost da
inhibiraju enzime; najaktivniji su bili genistein i viteksin (IC 50AChE 54,75 i 88,35 μg/mL;
IC 50BuChE 27,83 i 49,77 μg/mL). Uzimajući u obzir toksič nost hinolizidinskih alkaloida i slabu
antiholinesteraznu aktivnost AF, mož e se zaključ iti da je frakcija metanolnog ekstrakata
herbe bogata flavonoidima i oslobođena alkaloida (NAF), u pogledu bezbednosnog profila i
lekovitog potencijala dobar kandidat za dalja ispitivanja.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts
T1  - Sekundarni metaboliti i antiholinesterazna aktivnost herbe Chamaecytisus heuffelii subsp. jankae
VL  - 72
IS  - 4 suplement
SP  - S471
EP  - S472
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4585
ER  - 

@conference{
author = "Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) is a dwarfish shrub,
which range includes eastern part of the Balkan Peninsula (Albania, Bulgaria, Serbia) (1).
The aim of the investigation was to chemically characterize aerial flowering parts of this
plant: the dried methanol extract (TM), its alkaloid (AF) and non-alkaloid (NAF) fractions,
and to assess their ability and the ability of detected flavonoids to inhibit enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), in vitro in the Ellman
spectrophotometric test. Plant material was collected in eastern Serbia (Jelašnička Klisura
gorge), dried, powdered and extracted with methanol, after dichloromethane pre-extraction.
Using solvents of different polarities and pH value, AF (yield 14.35%) and NAF (yield
57.78%) were obtained from TM. By LC-MS analysis, flavonoids luteolin-8-C-
glucosylpentoside (159.81 mg/g), genistin (22.38 mg/g), vitexin (6.32 mg/g), rutin (4.25
mg/g), and genistein (0.86 mg/g) were detected and quantified in TM, while luteolin-8-C-
glucosylpentoside (212.70 mg/g), rutin (1.38 mg/g), and genistin (1.03 mg/g) in NAF. Using
GC-FID-MS analysis, quinolizidine alkaloids sparteine, 17-oksosparteine, and lupanine were
identified in AF. TM and NAF exhibited significant and comparable anti-cholinesterase
activity (IC50AChE 0.54 and 0.72 mg/mL; IC50BuChE 0.45 and 0.68 mg/mL). All detected
flavonoids evinced the ability to inhibit enzymes; the most active were genistein and vitexin
(IC50AChE 54.75 and 88.35 μg/mL; IC 50BuChE 27.83 and 49.77 μg/mL). Considering the
quinolizidine alkaloids toxicity and AF poor anti-cholinesterase activity, it might be
concluded, regarding safety profile and medical potential, that the fraction of the herb
methanol extract, flavonoid-rich and alkaloid-free (NAF), is good candidate for further
research., Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) je polužbun, koji
raste na području istočnog dela Balkanskog poluostrva (Albanija, Bugarska, Srbija) (1). Cilj
istraživanja bio je da se izvrši hemijska karakterizacija nadzemnih delova u cvetu ove biljke:
suvog metanolnog ekstrakta (TM), njegove alkaloidne (AF) i frakcije bez alkaloida (NAF), i da
se in vitro, u Ellmanov-om spektrofotomerijskom testu, ispita njihova sposobnost, kao i
sposobnost detektovanih flavonoida da inhibiraju enzime acetilholinesterazu (AChE) i
butirilholinesterazu (BChE). Biljni materijal je sakupljen u istočnoj Srbiji (Jelašnička klisura),
osušen, samleven i ekstrahovan metanolom, nakon pre-ekstrakcije dihlormetanom.
Primenom rastvarača različite polarnosti uz promenu pH vrednosti, iz TM su dobijene AF
(prinos 14,35%) i NAF (prinos 57,78%). LC-MS analizom, u TM detektovani su i
kvanitikovani flavonoidi luteolin-8-C-glukozilpentozid (159,81 mg/g), genistin (22,38 mg/g),
viteksin (6,32 mg/g) i rutin (4,25 mg/g) i genistein (0,86 mg/g), a u NAF luteolin-8-C-
glukozilpentozid (212,70 mg/g), rutin (1,38 mg/g) i genistin (1,03 mg/g). GC-FID-MS
analizom, u AF identifikovani su hinolizidinski alkaloidi spartein, 17-oksospartein i lupanin.
TM i NAF ispoljili su značajnu i uporedivu antiholinesteraznu aktivnost (IC50AChE 0,54 i 0,72
mg/mL; IC 50BChE 0,45 i 0,68 mg/mL). Svi detektovani flavonoidi su pokazali sposobnost da
inhibiraju enzime; najaktivniji su bili genistein i viteksin (IC 50AChE 54,75 i 88,35 μg/mL;
IC 50BuChE 27,83 i 49,77 μg/mL). Uzimajući u obzir toksič nost hinolizidinskih alkaloida i slabu
antiholinesteraznu aktivnost AF, mož e se zaključ iti da je frakcija metanolnog ekstrakata
herbe bogata flavonoidima i oslobođena alkaloida (NAF), u pogledu bezbednosnog profila i
lekovitog potencijala dobar kandidat za dalja ispitivanja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts, Sekundarni metaboliti i antiholinesterazna aktivnost herbe Chamaecytisus heuffelii subsp. jankae",
volume = "72",
number = "4 suplement",
pages = "S471-S472",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4585"
}

Milutinović, V., Niketić, M.,& Petrović, S.. (2022). Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S471-S472.
https://hdl.handle.net/21.15107/rcub_farfar_4585

Milutinović V, Niketić M, Petrović S. Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts. in Arhiv za farmaciju. 2022;72(4 suplement):S471-S472.
https://hdl.handle.net/21.15107/rcub_farfar_4585 .

Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S471-S472,
https://hdl.handle.net/21.15107/rcub_farfar_4585 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4505
AB  - The genus Hieracium L. s. str. (Cichorieae, Asteraceae) is one of the most taxonomically complex genera of flowering plants. Chemosystematic significance of triterpenes especially for Asteraceae genera is scarcely assessed. The subject of this research are 28 Hieracium species from Balkan Peninsula (mostly collected at Mt Durmitor, Montenegro): 12 principal belonging to sections Pannosa, Naegeliana, Drepanoidea and Villosa, and 16 hybridogenous originated from the species from those four sections and Glauciformia, Hieracium, Prenanthoidea and/or Italica. Previously, in dried dichloromethane extracts of flowering aerial plant parts, α-amyrin and α-amyrin acetate, as well as β-amyrin, β-amyrin acetate and/or lupeol acetate were identified and quantified using GC-FID-MS. The aim was to evaluate chemosystematic significance of these five triterpenes using multivariate statistical methods (principal components analysis-PCA and non-metric multidimensional scaling-nMDS). Statistical analysis supported the current taxonomical classification of the investigated species, despite overlapping of certain groups. In PCA, all five triterpenes significantly contributed to the differences between the species. Moreover, lupeol acetate could be a significant chemosystematic marker for the most of principal species belonging to the section Pannosa, as well as for some hybridogenous species between H. gymnocephalum Gris. ex Pant. and the members from the other sections.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula
SP  - 185
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4505
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "The genus Hieracium L. s. str. (Cichorieae, Asteraceae) is one of the most taxonomically complex genera of flowering plants. Chemosystematic significance of triterpenes especially for Asteraceae genera is scarcely assessed. The subject of this research are 28 Hieracium species from Balkan Peninsula (mostly collected at Mt Durmitor, Montenegro): 12 principal belonging to sections Pannosa, Naegeliana, Drepanoidea and Villosa, and 16 hybridogenous originated from the species from those four sections and Glauciformia, Hieracium, Prenanthoidea and/or Italica. Previously, in dried dichloromethane extracts of flowering aerial plant parts, α-amyrin and α-amyrin acetate, as well as β-amyrin, β-amyrin acetate and/or lupeol acetate were identified and quantified using GC-FID-MS. The aim was to evaluate chemosystematic significance of these five triterpenes using multivariate statistical methods (principal components analysis-PCA and non-metric multidimensional scaling-nMDS). Statistical analysis supported the current taxonomical classification of the investigated species, despite overlapping of certain groups. In PCA, all five triterpenes significantly contributed to the differences between the species. Moreover, lupeol acetate could be a significant chemosystematic marker for the most of principal species belonging to the section Pannosa, as well as for some hybridogenous species between H. gymnocephalum Gris. ex Pant. and the members from the other sections.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula",
pages = "185-185",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4505"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2022). Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 185-185.
https://hdl.handle.net/21.15107/rcub_farfar_4505

Milutinović V, Ušjak L, Niketić M, Petrović S. Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:185-185.
https://hdl.handle.net/21.15107/rcub_farfar_4505 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):185-185,
https://hdl.handle.net/21.15107/rcub_farfar_4505 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4504
AB  - The wood-sage (woodland germander) Teucrium scorodonia L. is widespread in European Atlantic coast and in the western part of Central Europe, with some isolated populations in Mediterranean. Recently discovered population on the territory of Serbia, in the valley of Štavica river (vicinity of Loznica), represents the most eastern enclave in the species range. Preliminary chemical analysis of aerial flowering parts and its dried ethanol and hydroethanol (70% v/v) extracts, and assessment of antioxidant potential of selected (hydroethanol) extract were performed. Using appropriate spectrophotometrical methods, total polyphenols in herb (3.48%, expressed as pyrogallol), ethanol and hydroethanol extracts (100.05 and 96.65 mg gallic acid equivalents - GAE/g), tannins in herb (0.96%, expressed as pyrogallol), ethanol and hydroethanol extracts (11.86 and 12.31 mg GAE/g), flavonoids in herb, ethanol and hydroethanol extracts (0.19%, 0.83% and 0.74%, expressed as hyperoside) were quantified. Also, in ethanol and hydroethanol extracts dihydroxycinnamic acid derivatives were determined (8.96% and 8.37%, expressed as acteoside). By LC-MS, in selected (hydroethanol) extract, four flavonoid and five phenylethanoid glycosides were detected; verbascoside (acteoside) was identified and quantified (4.42%) using external standard. This extract exhibited significant total antioxidant activity (1.37 mmol Fe2+/g) and moderate anti-DPPH potential (SC50=51.28 μg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4504
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "The wood-sage (woodland germander) Teucrium scorodonia L. is widespread in European Atlantic coast and in the western part of Central Europe, with some isolated populations in Mediterranean. Recently discovered population on the territory of Serbia, in the valley of Štavica river (vicinity of Loznica), represents the most eastern enclave in the species range. Preliminary chemical analysis of aerial flowering parts and its dried ethanol and hydroethanol (70% v/v) extracts, and assessment of antioxidant potential of selected (hydroethanol) extract were performed. Using appropriate spectrophotometrical methods, total polyphenols in herb (3.48%, expressed as pyrogallol), ethanol and hydroethanol extracts (100.05 and 96.65 mg gallic acid equivalents - GAE/g), tannins in herb (0.96%, expressed as pyrogallol), ethanol and hydroethanol extracts (11.86 and 12.31 mg GAE/g), flavonoids in herb, ethanol and hydroethanol extracts (0.19%, 0.83% and 0.74%, expressed as hyperoside) were quantified. Also, in ethanol and hydroethanol extracts dihydroxycinnamic acid derivatives were determined (8.96% and 8.37%, expressed as acteoside). By LC-MS, in selected (hydroethanol) extract, four flavonoid and five phenylethanoid glycosides were detected; verbascoside (acteoside) was identified and quantified (4.42%) using external standard. This extract exhibited significant total antioxidant activity (1.37 mmol Fe2+/g) and moderate anti-DPPH potential (SC50=51.28 μg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4504"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2022). Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_4504

Milutinović V, Ušjak L, Niketić M, Petrović S. Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_4504 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_4504 .

TY  - CONF
AU  - Milutinović, Violeta
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4449
AB  - The hawkweed genus Hieracium L. s. str. (Asteraceae) is one of the species-richest and
systematically the most complex genera of flowering plants. The genus traditional
classification, beside Hieracium, included, among others, subgenus Pilosella, which is
currently considered as separate genus. In folk medicine, application of some Hieracium s.
str. species was reported for various respiratory, gastrointestinal, urogenital, and some skin
disorders. The 28 species from the Balkan Peninsula were investigated, and majority was
collected on the Mt Durmitor (Montenegro), where the greatest diversity of taxa of this genus
was registered. In flowering aerial parts, composition of pharmaceutically significant
secondary metabolites - flavonoids, phenolic acids, sesquiterpene lactones and triterpenes
was established, and by multivariate statistical analysis their chemosystematic significance
was demonstrated. Corresponding metabolites isolation was performed using column
chromatography and semi-preparative liquid chromatography (LC-MS), identification using
mass spectrometry, NMR spectroscopy and refractometry, and extracts comparative
qualitative and quantitative analysis using LC-MS or gas chromatography (GC-FID-MS). In all
28 species, sesquiterpene lactones were identified for the first time. Three of them were
previously undescribed proline conjugates. Sesquiterpene lactone-amino acid conjugates are
quite rare in nature, and formerly were identified only in a few species of other Asteraceae
genera. Results obtained by investigation of pharmacological activities (in vitro antioxidant,
antimicrobial, cytotoxic, anticholinesterase, and in vivo antihyperalgesic and antiedematous),
as well as safety profiles of selected, chemically characterized extracts and/or secondary
metabolites, indicated the medicinal potential of the analysed plants and gave justification
for further research aiming to find new natural medicinal raw materials.
AB  - Rod Hieracium L. s. str. (jastrebnik, runjavica) (Asteraceae) je jedan od najvećih i
sistematski najsloženijih rodova skrivenosemenica. Tradicionalna klasifikacija roda je pored
podroda Hieracium uključivala, između ostalog, i podrod Pilosella, koji prema savremenoj
klasifikaciji ima status roda. Za pojedine vrste roda Hieracium s. str. je zabeležena primena u
narodnoj medicini kod različitih poremećaja respiratornog, gastrointestinalnog, urogenitalnog
trakta i kod nekih kožnih bolesti. Sprovedeno je istraživanje 28 vrsta sa Balkanskog
poluostrva, od kojih je najveć i broj sakupljen na planini Durmitor (Crna Gora), na kojoj je
konstatovan najveći diverzitet taksona roda. U nadzemnim delovima biljaka u cvetu ustanovljena
je kompozicija sekundarnih metabolita od značaja za farmaciju - flavonoida, fenolkarboksilnih
kiselina, seskviterpenskih laktona i triterpena, i multivarijantnom statističkom analizom
pokazan i njihov hemosistematski značaj. Izolovanje odgovarajućih metabolita izvršeno je
primenom hromatografije u koloni i semi-preparativne tečne hromatografije (LC-MS),
identifikacija primenom masene spektrometrije, NMR spektroskopije i refraktometrije, a uporedna
kvalitativna i kvantitativna analiza ekstrakata primenom LC-MS ili gasne hromatografije (GC-FID-
MS). Seskviterpenski laktoni su u svih 28 vrsta identifikovani po prvi put, od kojih su tri do
sada neopisani konjugati sa prolinom. Konjugati seskviterpenskih laktona sa
aminokiselinama su inače vrlo retki u prirodi i do sada su identifikovani samo u nekoliko
vrsta drugih rodova familije Asteraceae. Rezultati dobijeni ispitivanjem farmakološke
aktivnosti (in vitro antioksidantne, antimikrobne, citotoksične, antiholinesterazne, i in vivo
antihiperalgezijske i antiedematozne), kao i rezultati ispitivanja bezbednosti primene za
odabrane, hemijski okarakterisane ekstrakte i/ili sekundarne metabolite ukazali su na
lekoviti potencijal ispitivanih biljaka i opravdanost daljih istraživanja u cilju iznalaženja
novih prirodnih lekovitih sirovina.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential
T1  - Vrste roda Hieracium s. str. sa Balkanskog poluostrva: sekundarni metaboliti i farmakološki potencijal
VL  - 72
IS  - 4 suplement
SP  - S64
EP  - S65
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4449
ER  - 

@conference{
author = "Milutinović, Violeta",
year = "2022",
abstract = "The hawkweed genus Hieracium L. s. str. (Asteraceae) is one of the species-richest and
systematically the most complex genera of flowering plants. The genus traditional
classification, beside Hieracium, included, among others, subgenus Pilosella, which is
currently considered as separate genus. In folk medicine, application of some Hieracium s.
str. species was reported for various respiratory, gastrointestinal, urogenital, and some skin
disorders. The 28 species from the Balkan Peninsula were investigated, and majority was
collected on the Mt Durmitor (Montenegro), where the greatest diversity of taxa of this genus
was registered. In flowering aerial parts, composition of pharmaceutically significant
secondary metabolites - flavonoids, phenolic acids, sesquiterpene lactones and triterpenes
was established, and by multivariate statistical analysis their chemosystematic significance
was demonstrated. Corresponding metabolites isolation was performed using column
chromatography and semi-preparative liquid chromatography (LC-MS), identification using
mass spectrometry, NMR spectroscopy and refractometry, and extracts comparative
qualitative and quantitative analysis using LC-MS or gas chromatography (GC-FID-MS). In all
28 species, sesquiterpene lactones were identified for the first time. Three of them were
previously undescribed proline conjugates. Sesquiterpene lactone-amino acid conjugates are
quite rare in nature, and formerly were identified only in a few species of other Asteraceae
genera. Results obtained by investigation of pharmacological activities (in vitro antioxidant,
antimicrobial, cytotoxic, anticholinesterase, and in vivo antihyperalgesic and antiedematous),
as well as safety profiles of selected, chemically characterized extracts and/or secondary
metabolites, indicated the medicinal potential of the analysed plants and gave justification
for further research aiming to find new natural medicinal raw materials., Rod Hieracium L. s. str. (jastrebnik, runjavica) (Asteraceae) je jedan od najvećih i
sistematski najsloženijih rodova skrivenosemenica. Tradicionalna klasifikacija roda je pored
podroda Hieracium uključivala, između ostalog, i podrod Pilosella, koji prema savremenoj
klasifikaciji ima status roda. Za pojedine vrste roda Hieracium s. str. je zabeležena primena u
narodnoj medicini kod različitih poremećaja respiratornog, gastrointestinalnog, urogenitalnog
trakta i kod nekih kožnih bolesti. Sprovedeno je istraživanje 28 vrsta sa Balkanskog
poluostrva, od kojih je najveć i broj sakupljen na planini Durmitor (Crna Gora), na kojoj je
konstatovan najveći diverzitet taksona roda. U nadzemnim delovima biljaka u cvetu ustanovljena
je kompozicija sekundarnih metabolita od značaja za farmaciju - flavonoida, fenolkarboksilnih
kiselina, seskviterpenskih laktona i triterpena, i multivarijantnom statističkom analizom
pokazan i njihov hemosistematski značaj. Izolovanje odgovarajućih metabolita izvršeno je
primenom hromatografije u koloni i semi-preparativne tečne hromatografije (LC-MS),
identifikacija primenom masene spektrometrije, NMR spektroskopije i refraktometrije, a uporedna
kvalitativna i kvantitativna analiza ekstrakata primenom LC-MS ili gasne hromatografije (GC-FID-
MS). Seskviterpenski laktoni su u svih 28 vrsta identifikovani po prvi put, od kojih su tri do
sada neopisani konjugati sa prolinom. Konjugati seskviterpenskih laktona sa
aminokiselinama su inače vrlo retki u prirodi i do sada su identifikovani samo u nekoliko
vrsta drugih rodova familije Asteraceae. Rezultati dobijeni ispitivanjem farmakološke
aktivnosti (in vitro antioksidantne, antimikrobne, citotoksične, antiholinesterazne, i in vivo
antihiperalgezijske i antiedematozne), kao i rezultati ispitivanja bezbednosti primene za
odabrane, hemijski okarakterisane ekstrakte i/ili sekundarne metabolite ukazali su na
lekoviti potencijal ispitivanih biljaka i opravdanost daljih istraživanja u cilju iznalaženja
novih prirodnih lekovitih sirovina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential, Vrste roda Hieracium s. str. sa Balkanskog poluostrva: sekundarni metaboliti i farmakološki potencijal",
volume = "72",
number = "4 suplement",
pages = "S64-S65",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4449"
}

Milutinović, V.. (2022). Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S64-S65.
https://hdl.handle.net/21.15107/rcub_farfar_4449

Milutinović V. Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential. in Arhiv za farmaciju. 2022;72(4 suplement):S64-S65.
https://hdl.handle.net/21.15107/rcub_farfar_4449 .

Milutinović, Violeta, "Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S64-S65,
https://hdl.handle.net/21.15107/rcub_farfar_4449 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana D.
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4172
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones
VL  - 19
IS  - 7
SP  - e202200326
DO  - 10.1002/cbdv.202200326
ER  - 

@article{
author = "Milutinović, Violeta and Matić, Ivana Z. and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana D. and Ušjak, Ljuboš and Niketić, Marjan S. and Petrović, Silvana",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones",
volume = "19",
number = "7",
pages = "e202200326",
doi = "10.1002/cbdv.202200326"
}

Milutinović, V., Matić, I. Z., Stanojković, T., Soković, M., Ćirić, A. D., Ušjak, L., Niketić, M. S.,& Petrović, S.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović V, Matić IZ, Stanojković T, Soković M, Ćirić AD, Ušjak L, Niketić MS, Petrović S. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta, Matić, Ivana Z., Stanojković, Tatjana, Soković, Marina, Ćirić, Ana D., Ušjak, Ljuboš, Niketić, Marjan S., Petrović, Silvana, "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones" in Chemistry and Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4423
AB  - Stachys milanii Petrović (Lamiaceae) is an endemic of central and eastern part of Balkan Peninsula, growing mainly in river valleys on semi-saline soils, often along the edge of cereal fields and abandoned arable lands. It is a critically endangered and strictly protected species in Serbia, with just one proven habitat (1). So far it was investigated on herb essential oil composition and antimicrobial activity, and on fatty acid composition of seeds (2). 
Aim of this work was to investigate antiradical potential and phenolic constituents of dry methanol herb extract. Aerial flowering parts (herb) of S. milanii were collected in North Macedonia (Ovče Pole). Powdered, dried plant material was extracted by bimaceration with chloroform, and then with methanol. Obtained dry methanol extract was used for further analysis. Using appropriate spectrophotometric procedure strong, concentration-dependant anti-DPPH activity of extract was observed (SC50=34.15 μg/mL). Also spectrophotometrically, using Folin-Ciocalteu reagent, significant total phenolics content (0,47 mg gallic acid/mg dry extract) was determined. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of one phenolic acid, four phenylethanol glycosides, eight flavonoid glycosides derivatives of 8-hydroxyflavones, and one apigenin glycoside. Using commercial standards in extract were identified and quantified: chlorogenic acid (1.63%) and acteoside (2.65%), as the main phenylethanoid. In 8-hydroxyflavones fraction six hypolaetine and two isoscutellarein derivatives were present; using compounds previously isolated from S. alpina subsp. dinarica Murb. herb, 4'-O-methylhypolaetine-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside and isoscutellarein-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside were identified, representing the most prominent flavonoids in investigated extract, respectively.  


References
1. Niketić M, Diklić N. Stachys milanii Petrović. In: Stevanović V. (Ed.) Red Book of Flora of Serbia 1 - Extinct and Critically Endangered Taxa. Belgrade: Ministry for the Protection of Natural Resources and the Environment, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia. 1999; pp. 211-3, 466-7.
2. Stojanovic G, Ligon AP, Smelcerovic A, Lazarevic J, Spiteller M, Palic R. Fatty acids of Stachys milanii seeds. Chem Nat Comp 2007;43(4):380-3.


Acknowledgements
This research was funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161.
AB  - Stachys milanii Petrović (Lamiaceae) je endemit centralnog i istočnog dela Balkanskog
poluostrva koji raste pretežno u dolinama reka na poluslanim zemljištima, često i po obodu
žitnih polja i po napuštenim oranicama. U Republici Srbiji je kritično ugrožena i strogo
zaštićena vrsta, sa samo jednim zabeleženim staništem (1). Biljka je do sada ispitivana u
pogledu sastava i antimikrobne aktivnosti etarskog ulja herbe i kompozije masnih kiselina
semena (2). Cilj ovog rada bio je da se ispitaju antiradikalski potencijal i fenolni sastojci
suvog metanolnog ekstrakta herbe. Nadzemni delovi S. milanii u cvetu prikupljeni su u
Severnoj Makedoniji (Ovče Pole). Sprašen, osušen biljni materijal je ekstrahovan posupkom
bimaceracije najpre hloroformom, a zatim metanolom. Dobijeni suvi metanolni ekstrakt
korišćen je za dalju analizu. Odgovarajućim spektrofotometrijskim postupkom utvrđena je
snažna, koncentraciono-zavisna anti-DPPH aktivnost ekstrakta (SC50=34,15 μg/mL).
Spektrofotometrijski, korišćenjem Folin-Ciocalteu reagensa, utvrđen je i značajan sadržaj
ukupnih polifenola (0,47 mg galne kiseline/mg suvog ekstrakta). U TLC-DPPH testu uočene
žute zone ukazale su na prisustvo većeg broja jedinjenja koja snažno neutrališu ljubičasti
DPPH-radikal. LC-MS analizom u ekstraktu su detektovani jedna fenolkarboksilna kiselina,
četiri feniletanska heterozida, osam flavonoidnih heterozida derivata 8-hidroksiflavona i
jedan heterozid apigenina. Korišćenjem komercijalnih standarda identifikovni su i
kvantifikovani hlorogenska kiselina (1,63%) i akteozid (2,65%), kao glavni feniletanski
heterozid. Frakciju derivata 8-hidroksiflavona činilo je šest heterozida hipolaetina i dva
heterozida izoskutelareina; korišćenjem jedinjenja prethodno izolovanih iz herbe S. alpina
subsp. dinarica Murb., identifikovani su 4'-O-metilhipolaetin-7-O-[6'''-acetilalozil(12)]-
glukopiranozid i izoskutelarein 7-O-[6'''-acetilalozil(12)]-glukopiranozid, koji su ujedno
predstavljali i najzastupljnije flavonoide u ekstraktu, redom.
PB  - Pharmaceutical Association of Serbia
C3  - Arhiv za farmaciju
T1  - Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract
T1  - Antiradikalska aktivnost i fenolni sastojci metanolnog ekstrakta herbe Stachys milanii
VL  - 72
IS  - 4 suplement
SP  - S463
EP  - S464
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4423
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Stachys milanii Petrović (Lamiaceae) is an endemic of central and eastern part of Balkan Peninsula, growing mainly in river valleys on semi-saline soils, often along the edge of cereal fields and abandoned arable lands. It is a critically endangered and strictly protected species in Serbia, with just one proven habitat (1). So far it was investigated on herb essential oil composition and antimicrobial activity, and on fatty acid composition of seeds (2). 
Aim of this work was to investigate antiradical potential and phenolic constituents of dry methanol herb extract. Aerial flowering parts (herb) of S. milanii were collected in North Macedonia (Ovče Pole). Powdered, dried plant material was extracted by bimaceration with chloroform, and then with methanol. Obtained dry methanol extract was used for further analysis. Using appropriate spectrophotometric procedure strong, concentration-dependant anti-DPPH activity of extract was observed (SC50=34.15 μg/mL). Also spectrophotometrically, using Folin-Ciocalteu reagent, significant total phenolics content (0,47 mg gallic acid/mg dry extract) was determined. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of one phenolic acid, four phenylethanol glycosides, eight flavonoid glycosides derivatives of 8-hydroxyflavones, and one apigenin glycoside. Using commercial standards in extract were identified and quantified: chlorogenic acid (1.63%) and acteoside (2.65%), as the main phenylethanoid. In 8-hydroxyflavones fraction six hypolaetine and two isoscutellarein derivatives were present; using compounds previously isolated from S. alpina subsp. dinarica Murb. herb, 4'-O-methylhypolaetine-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside and isoscutellarein-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside were identified, representing the most prominent flavonoids in investigated extract, respectively.  


References
1. Niketić M, Diklić N. Stachys milanii Petrović. In: Stevanović V. (Ed.) Red Book of Flora of Serbia 1 - Extinct and Critically Endangered Taxa. Belgrade: Ministry for the Protection of Natural Resources and the Environment, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia. 1999; pp. 211-3, 466-7.
2. Stojanovic G, Ligon AP, Smelcerovic A, Lazarevic J, Spiteller M, Palic R. Fatty acids of Stachys milanii seeds. Chem Nat Comp 2007;43(4):380-3.


Acknowledgements
This research was funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161., Stachys milanii Petrović (Lamiaceae) je endemit centralnog i istočnog dela Balkanskog
poluostrva koji raste pretežno u dolinama reka na poluslanim zemljištima, često i po obodu
žitnih polja i po napuštenim oranicama. U Republici Srbiji je kritično ugrožena i strogo
zaštićena vrsta, sa samo jednim zabeleženim staništem (1). Biljka je do sada ispitivana u
pogledu sastava i antimikrobne aktivnosti etarskog ulja herbe i kompozije masnih kiselina
semena (2). Cilj ovog rada bio je da se ispitaju antiradikalski potencijal i fenolni sastojci
suvog metanolnog ekstrakta herbe. Nadzemni delovi S. milanii u cvetu prikupljeni su u
Severnoj Makedoniji (Ovče Pole). Sprašen, osušen biljni materijal je ekstrahovan posupkom
bimaceracije najpre hloroformom, a zatim metanolom. Dobijeni suvi metanolni ekstrakt
korišćen je za dalju analizu. Odgovarajućim spektrofotometrijskim postupkom utvrđena je
snažna, koncentraciono-zavisna anti-DPPH aktivnost ekstrakta (SC50=34,15 μg/mL).
Spektrofotometrijski, korišćenjem Folin-Ciocalteu reagensa, utvrđen je i značajan sadržaj
ukupnih polifenola (0,47 mg galne kiseline/mg suvog ekstrakta). U TLC-DPPH testu uočene
žute zone ukazale su na prisustvo većeg broja jedinjenja koja snažno neutrališu ljubičasti
DPPH-radikal. LC-MS analizom u ekstraktu su detektovani jedna fenolkarboksilna kiselina,
četiri feniletanska heterozida, osam flavonoidnih heterozida derivata 8-hidroksiflavona i
jedan heterozid apigenina. Korišćenjem komercijalnih standarda identifikovni su i
kvantifikovani hlorogenska kiselina (1,63%) i akteozid (2,65%), kao glavni feniletanski
heterozid. Frakciju derivata 8-hidroksiflavona činilo je šest heterozida hipolaetina i dva
heterozida izoskutelareina; korišćenjem jedinjenja prethodno izolovanih iz herbe S. alpina
subsp. dinarica Murb., identifikovani su 4'-O-metilhipolaetin-7-O-[6'''-acetilalozil(12)]-
glukopiranozid i izoskutelarein 7-O-[6'''-acetilalozil(12)]-glukopiranozid, koji su ujedno
predstavljali i najzastupljnije flavonoide u ekstraktu, redom.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract, Antiradikalska aktivnost i fenolni sastojci metanolnog ekstrakta herbe Stachys milanii",
volume = "72",
number = "4 suplement",
pages = "S463-S464",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4423"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2022). Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 72(4 suplement), S463-S464.
https://hdl.handle.net/21.15107/rcub_farfar_4423

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract. in Arhiv za farmaciju. 2022;72(4 suplement):S463-S464.
https://hdl.handle.net/21.15107/rcub_farfar_4423 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S463-S464,
https://hdl.handle.net/21.15107/rcub_farfar_4423 .

TY  - THES
AU  - Milutinović, Violeta
PY  - 2021
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8442
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24744/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49042953
UR  - https://nardus.mpn.gov.rs/handle/123456789/18845
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4039
AB  - hibridogenih).Semipreparativnim LC-MS metodama, iz MeOH ekstrakta cvasti H. calophyllum izolovana sučetiri seskviterpenska laktona (krepizid E i tri nova jedinjenja: 8-epi-ikserisamin A, kalofilamin A ikalofilamin B), a iz MeOH ekstrakta podzemnih organa H. scheppigianum pet fenolkarboksilnih(mono- ili di-O-kafeoilhina) kiselina (identifikacija na osnovu NMR i MS spektara).Ukupno četiri seskviterpenska laktona, sedam mono- ili di-O-kafeoilhina kiselina i 19flavonoida (luteolin, apigenin, diosmetin, 15 njihovih heterozida i heterozid kvercetina)kvantifikovani su u suvim MeOH ekstraktima ispitivanih podzemnih organa dve vrste i herbi 28vrsta LC-MS metodama, i pet triterpena (derivata ursana, oleana i lupana) u suvim CH2Cl2ekstraktima herbi 28 vrsta GC-FID-MS metodom (identifikacija pomoću standarda, NMR, MS i/iliUV spektara). Hemosistematski značaj jedinjenja identifikovanih u herbama 28 vrsta utvrđen jemultivarijantnim statističkim metodama (PCA, nMDS i UPGMA).Suvi MeOH ekstrakti herbi su in vitro pokazali značajan ukupni antioksidantni potencijal isposobnost neutralizacije slobodnih DPPH•, ABTS•+ i OH• radikala (28 vrsta) i inhibicijeacetilholinesteraze (27 vrsta) i butirilholinesteraze (7 vrsta). Među testiranih 12 jedinjenja, najboljuantiholinesteraznu aktivnost ispoljila su tri flavonoidna aglikona i 8-epi-ikserisamin A. Odabranisuvi MeOH i CH2Cl2 ekstrakti herbi šest vrsta pokazali su antimikrobnu aktivnost prema 18, a 8-epi-ikserisamin A i krepizid E prema 4 mikroorganizma. Isti odabrani izolati ispoljili sucitotoksični efekat prema tri, odnosno jednoj tumorskoj ćelijskoj liniji, uz zanemarljivu inhibicijurasta zdravih ćelija. Tri odabrana MeOH i CH2Cl2 ekstrakta su u modelu inflamacije šape pacovaizazvane karageninom pokazala antihiperalgezijsku, ali ne i antiedematoznu aktivnost; bezbednostprimene dva ekstrakta koji su ispoljili značajnu antihiperalgezijsku aktivnost pokazana je in vivorotarod i testom akutne toksičnosti.Ključne reči:
AB  - nonhybridogenousand 16 hybridogenous).Using semipreparative LC-MS methods, four sesquiterpene lactones (crepiside E and three newcompounds: 8-epiixerisamine A, calophyllamine A and calophyllamine B) were isolated from theMeOH extract of H. calophyllum flowering heads, and five phenolic (mono- or di-Ocaffeoylquinic)acids from the MeOH extract of H. scheppigianum underground parts(identification based on NMR and MS spectra).Total of four sesquiterpene lactones, seven mono- or di-O-caffeoylquinic acids and 19flavonoids (luteolin, apigenin, diosmetin, their 15 glycosides and one quercetin glycoside) werequantified in the dried MeOH extracts of the investigated underground parts of two species andherbs of 28 species, using LC-MS methods, and five triterpenes (ursane, oleane and lupanederivatives) in the dried CH2Cl2 extracts of the herbs of 28 species, using GC-FID-MS method(identification using standards, NMR, MS and/or UV spectra). Chemosystematic significance ofthe compounds identified in the herbs of 28 species was determined using multivariate statistics(PCA, nMDS and UPGMA).Dried MeOH herb extracts in vitro exhibited significant total antioxidant activity andscavenging of DPPH•, ABTS•+ and OH• radicals (28 species), as well as the ability to inhibitacetylcholinesterase (27 species) and butyrylcholinesterase (7 species). Among 12 testedcompounds, three flavonoid aglycones and 8-epiixerisamine A exhibited the highestanticholinesterase activity. Selected dried MeOH and CH2Cl2 herb extracts of six species showedantimicrobial activity against 18, whereas 8-epiixerisamine A and crepiside E against fourmicroorganisms. The same selected isolates exhibited cytotoxic activity against three and onecancer cell lines, respectively, with negligible inhibition of the normal cells growth. In thecarrageenan-induced localized inflammation model in rats, three selected MeOH and CH2Cl2extracts exhibited antihyperalgesic, but not antiedematous activity; safety of the oral administrationof two extracts that exhibited significant antihyperalgesic activity was demonstrated in vivo inrotarod test and acute oral toxicity study.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18845
ER  - 

@phdthesis{
author = "Milutinović, Violeta",
year = "2021",
abstract = "hibridogenih).Semipreparativnim LC-MS metodama, iz MeOH ekstrakta cvasti H. calophyllum izolovana sučetiri seskviterpenska laktona (krepizid E i tri nova jedinjenja: 8-epi-ikserisamin A, kalofilamin A ikalofilamin B), a iz MeOH ekstrakta podzemnih organa H. scheppigianum pet fenolkarboksilnih(mono- ili di-O-kafeoilhina) kiselina (identifikacija na osnovu NMR i MS spektara).Ukupno četiri seskviterpenska laktona, sedam mono- ili di-O-kafeoilhina kiselina i 19flavonoida (luteolin, apigenin, diosmetin, 15 njihovih heterozida i heterozid kvercetina)kvantifikovani su u suvim MeOH ekstraktima ispitivanih podzemnih organa dve vrste i herbi 28vrsta LC-MS metodama, i pet triterpena (derivata ursana, oleana i lupana) u suvim CH2Cl2ekstraktima herbi 28 vrsta GC-FID-MS metodom (identifikacija pomoću standarda, NMR, MS i/iliUV spektara). Hemosistematski značaj jedinjenja identifikovanih u herbama 28 vrsta utvrđen jemultivarijantnim statističkim metodama (PCA, nMDS i UPGMA).Suvi MeOH ekstrakti herbi su in vitro pokazali značajan ukupni antioksidantni potencijal isposobnost neutralizacije slobodnih DPPH•, ABTS•+ i OH• radikala (28 vrsta) i inhibicijeacetilholinesteraze (27 vrsta) i butirilholinesteraze (7 vrsta). Među testiranih 12 jedinjenja, najboljuantiholinesteraznu aktivnost ispoljila su tri flavonoidna aglikona i 8-epi-ikserisamin A. Odabranisuvi MeOH i CH2Cl2 ekstrakti herbi šest vrsta pokazali su antimikrobnu aktivnost prema 18, a 8-epi-ikserisamin A i krepizid E prema 4 mikroorganizma. Isti odabrani izolati ispoljili sucitotoksični efekat prema tri, odnosno jednoj tumorskoj ćelijskoj liniji, uz zanemarljivu inhibicijurasta zdravih ćelija. Tri odabrana MeOH i CH2Cl2 ekstrakta su u modelu inflamacije šape pacovaizazvane karageninom pokazala antihiperalgezijsku, ali ne i antiedematoznu aktivnost; bezbednostprimene dva ekstrakta koji su ispoljili značajnu antihiperalgezijsku aktivnost pokazana je in vivorotarod i testom akutne toksičnosti.Ključne reči:, nonhybridogenousand 16 hybridogenous).Using semipreparative LC-MS methods, four sesquiterpene lactones (crepiside E and three newcompounds: 8-epiixerisamine A, calophyllamine A and calophyllamine B) were isolated from theMeOH extract of H. calophyllum flowering heads, and five phenolic (mono- or di-Ocaffeoylquinic)acids from the MeOH extract of H. scheppigianum underground parts(identification based on NMR and MS spectra).Total of four sesquiterpene lactones, seven mono- or di-O-caffeoylquinic acids and 19flavonoids (luteolin, apigenin, diosmetin, their 15 glycosides and one quercetin glycoside) werequantified in the dried MeOH extracts of the investigated underground parts of two species andherbs of 28 species, using LC-MS methods, and five triterpenes (ursane, oleane and lupanederivatives) in the dried CH2Cl2 extracts of the herbs of 28 species, using GC-FID-MS method(identification using standards, NMR, MS and/or UV spectra). Chemosystematic significance ofthe compounds identified in the herbs of 28 species was determined using multivariate statistics(PCA, nMDS and UPGMA).Dried MeOH herb extracts in vitro exhibited significant total antioxidant activity andscavenging of DPPH•, ABTS•+ and OH• radicals (28 species), as well as the ability to inhibitacetylcholinesterase (27 species) and butyrylcholinesterase (7 species). Among 12 testedcompounds, three flavonoid aglycones and 8-epiixerisamine A exhibited the highestanticholinesterase activity. Selected dried MeOH and CH2Cl2 herb extracts of six species showedantimicrobial activity against 18, whereas 8-epiixerisamine A and crepiside E against fourmicroorganisms. The same selected isolates exhibited cytotoxic activity against three and onecancer cell lines, respectively, with negligible inhibition of the normal cells growth. In thecarrageenan-induced localized inflammation model in rats, three selected MeOH and CH2Cl2extracts exhibited antihyperalgesic, but not antiedematous activity; safety of the oral administrationof two extracts that exhibited significant antihyperalgesic activity was demonstrated in vivo inrotarod test and acute oral toxicity study.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18845"
}

Milutinović, V.. (2021). Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_18845

Milutinović V. Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Универзитет у Београду. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18845 .

Milutinović, Violeta, "Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva" in Универзитет у Београду (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18845 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Milutinović, Violeta
AU  - Đorđić Crnogorac, Marija J.
AU  - Stanojković, Tatjana P.
AU  - Niketić, Marjan S.
AU  - Kukić-Marković, Jelena
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3957
AB  - Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.
PB  - John Wiley and Sons Inc
T2  - Chemistry & Biodiversity
T1  - Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition
VL  - 18
IS  - 10
DO  - 10.1002/cbdv.202100446
ER  - 

@article{
author = "Ušjak, Ljuboš and Milutinović, Violeta and Đorđić Crnogorac, Marija J. and Stanojković, Tatjana P. and Niketić, Marjan S. and Kukić-Marković, Jelena and Petrović, Silvana",
year = "2021",
abstract = "Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry & Biodiversity",
title = "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition",
volume = "18",
number = "10",
doi = "10.1002/cbdv.202100446"
}

Ušjak, L., Milutinović, V., Đorđić Crnogorac, M. J., Stanojković, T. P., Niketić, M. S., Kukić-Marković, J.,& Petrović, S.. (2021). Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity
John Wiley and Sons Inc., 18(10).
https://doi.org/10.1002/cbdv.202100446

Ušjak L, Milutinović V, Đorđić Crnogorac MJ, Stanojković TP, Niketić MS, Kukić-Marković J, Petrović S. Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity. 2021;18(10).
doi:10.1002/cbdv.202100446 .

Ušjak, Ljuboš, Milutinović, Violeta, Đorđić Crnogorac, Marija J., Stanojković, Tatjana P., Niketić, Marjan S., Kukić-Marković, Jelena, Petrović, Silvana, "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition" in Chemistry & Biodiversity, 18, no. 10 (2021),
https://doi.org/10.1002/cbdv.202100446 . .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Pecikoza, Uroš
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3641
AB  - Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.
PB  - Taylor & Francis Ltd
T2  - Natural Product Research
T1  - Investigation of antihyperalgesic and antiedematous activities of three Hieracium species
VL  - 35
IS  - 23
SP  - 5384
EP  - 5388
DO  - 10.1080/14786419.2020.1768086
ER  - 

@article{
author = "Milutinović, Violeta and Pecikoza, Uroš and Tomić, Maja and Stepanović-Petrović, Radica and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana",
year = "2021",
abstract = "Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.",
publisher = "Taylor & Francis Ltd",
journal = "Natural Product Research",
title = "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species",
volume = "35",
number = "23",
pages = "5384-5388",
doi = "10.1080/14786419.2020.1768086"
}

Milutinović, V., Pecikoza, U., Tomić, M., Stepanović-Petrović, R., Niketić, M., Ušjak, L.,& Petrović, S.. (2021). Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research
Taylor & Francis Ltd., 35(23), 5384-5388.
https://doi.org/10.1080/14786419.2020.1768086

Milutinović V, Pecikoza U, Tomić M, Stepanović-Petrović R, Niketić M, Ušjak L, Petrović S. Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research. 2021;35(23):5384-5388.
doi:10.1080/14786419.2020.1768086 .

Milutinović, Violeta, Pecikoza, Uroš, Tomić, Maja, Stepanović-Petrović, Radica, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species" in Natural Product Research, 35, no. 23 (2021):5384-5388,
https://doi.org/10.1080/14786419.2020.1768086 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Pecikoza, Uroš
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5281
AB  - Rod Hieracium s. str. (Asteraceae) je autohton na teritoriji Evroazije, severne Afrike i Severne Amerike, sa velikim brojem taksona rasprostranjenih u Evropi. ...
AB  - Genus Hieracium s. str. (Asteraceae) is autochthonous in Eurasia, North Africa and North America, with the vast majority of taxa being distributed in Europe. ...
PB  - Srpsko farmakološko društvo (SFD)
C3  - 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem. Novi Sad, 18-21.09.2019. Zbornik kratkih sadržaja radova
T1  - Ispitivanje antihiperalgezijske i antiedematozne aktivnosti ekstrakata nadzemnih delova u cvetu tri vrste roda Hieracium
T1  - Investigation of antihyperalgesic and antiedematous activities of the flowering aerial parts extracts of three Hieracium species
SP  - 176
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5281
ER  - 

@conference{
author = "Milutinović, Violeta and Pecikoza, Uroš and Tomić, Maja and Stepanović-Petrović, Radica and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana",
year = "2019",
abstract = "Rod Hieracium s. str. (Asteraceae) je autohton na teritoriji Evroazije, severne Afrike i Severne Amerike, sa velikim brojem taksona rasprostranjenih u Evropi. ..., Genus Hieracium s. str. (Asteraceae) is autochthonous in Eurasia, North Africa and North America, with the vast majority of taxa being distributed in Europe. ...",
publisher = "Srpsko farmakološko društvo (SFD)",
journal = "14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem. Novi Sad, 18-21.09.2019. Zbornik kratkih sadržaja radova",
title = "Ispitivanje antihiperalgezijske i antiedematozne aktivnosti ekstrakata nadzemnih delova u cvetu tri vrste roda Hieracium, Investigation of antihyperalgesic and antiedematous activities of the flowering aerial parts extracts of three Hieracium species",
pages = "176-177",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5281"
}

Milutinović, V., Pecikoza, U., Tomić, M., Stepanović-Petrović, R., Niketić, M., Ušjak, L.,& Petrović, S.. (2019). Ispitivanje antihiperalgezijske i antiedematozne aktivnosti ekstrakata nadzemnih delova u cvetu tri vrste roda Hieracium. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem. Novi Sad, 18-21.09.2019. Zbornik kratkih sadržaja radova
Srpsko farmakološko društvo (SFD)., 176-177.
https://hdl.handle.net/21.15107/rcub_farfar_5281

Milutinović V, Pecikoza U, Tomić M, Stepanović-Petrović R, Niketić M, Ušjak L, Petrović S. Ispitivanje antihiperalgezijske i antiedematozne aktivnosti ekstrakata nadzemnih delova u cvetu tri vrste roda Hieracium. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem. Novi Sad, 18-21.09.2019. Zbornik kratkih sadržaja radova. 2019;:176-177.
https://hdl.handle.net/21.15107/rcub_farfar_5281 .

Milutinović, Violeta, Pecikoza, Uroš, Tomić, Maja, Stepanović-Petrović, Radica, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Ispitivanje antihiperalgezijske i antiedematozne aktivnosti ekstrakata nadzemnih delova u cvetu tri vrste roda Hieracium" in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem. Novi Sad, 18-21.09.2019. Zbornik kratkih sadržaja radova (2019):176-177,
https://hdl.handle.net/21.15107/rcub_farfar_5281 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5110
AB  - The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5110
ER  - 

@conference{
author = "Milutinović, Violeta and Petrović, Predrag and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5110"
}

Milutinović, V., Petrović, P., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2019). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110

Milutinović V, Petrović P, Klaus A, Ušjak L, Niketić M, Petrović S. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

Milutinović, Violeta, Petrović, Predrag, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Matić, Ivana
AU  - Grozdanić-Stanisavljević, Nađa
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5109
AB  - Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones
SP  - 162
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5109
ER  - 

@conference{
author = "Milutinović, Violeta and Matić, Ivana and Grozdanić-Stanisavljević, Nađa and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones",
pages = "162-163",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5109"
}

Milutinović, V., Matić, I., Grozdanić-Stanisavljević, N., Stanojković, T., Soković, M., Ćirić, A., Niketić, M.,& Petrović, S.. (2019). Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109

Milutinović V, Matić I, Grozdanić-Stanisavljević N, Stanojković T, Soković M, Ćirić A, Niketić M, Petrović S. Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

Milutinović, Violeta, Matić, Ivana, Grozdanić-Stanisavljević, Nađa, Stanojković, Tatjana, Soković, Marina, Ćirić, Ana, Niketić, Marjan, Petrović, Silvana, "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):162-163,
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Jovanović, Aleksandra
AU  - Simović, Milica
AU  - Milutinović, Violeta
AU  - Kozarski, Maja
AU  - Petković, Miloš
AU  - Cvetković, Anka
AU  - Klaus, Anita
AU  - Bugarski, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5105
AB  - Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.
PB  - Serbian Biological Society
T2  - Archives Biological Sciences
T1  - The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products
VL  - 71
IS  - 4
SP  - 721
EP  - 733
DO  - 10.2298/ABS190725055P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Jovanović, Aleksandra and Simović, Milica and Milutinović, Violeta and Kozarski, Maja and Petković, Miloš and Cvetković, Anka and Klaus, Anita and Bugarski, Branko",
year = "2019",
abstract = "Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.",
publisher = "Serbian Biological Society",
journal = "Archives Biological Sciences",
title = "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products",
volume = "71",
number = "4",
pages = "721-733",
doi = "10.2298/ABS190725055P"
}

Petrović, P., Ivanović, K., Jovanović, A., Simović, M., Milutinović, V., Kozarski, M., Petković, M., Cvetković, A., Klaus, A.,& Bugarski, B.. (2019). The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives Biological Sciences
Serbian Biological Society., 71(4), 721-733.
https://doi.org/10.2298/ABS190725055P

Petrović P, Ivanović K, Jovanović A, Simović M, Milutinović V, Kozarski M, Petković M, Cvetković A, Klaus A, Bugarski B. The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives Biological Sciences. 2019;71(4):721-733.
doi:10.2298/ABS190725055P .

Petrović, Predrag, Ivanović, Katarina, Jovanović, Aleksandra, Simović, Milica, Milutinović, Violeta, Kozarski, Maja, Petković, Miloš, Cvetković, Anka, Klaus, Anita, Bugarski, Branko, "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products" in Archives Biological Sciences, 71, no. 4 (2019):721-733,
https://doi.org/10.2298/ABS190725055P . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Stanojković, Tatjana
AU  - Đorđić Crnogorac, Marija
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5258
AB  - Pyrus × velenovskyi Dostálek (Rosaceae) [P. pyraster (L.) Burgsd. × P. spinosa Forssk.] is a deciduous tree up to 5 m height. Leaf-blades 2.2–4.8 × 1.3–2.7 cm, ± glabrate, ellip tic, gradually narrowed to a rather long petiole. Th is natural hybrid plant was described from Bulgaria, and has been also found in Serbia. The objective of this work was to investigate the phenolic profile, as well as in vitro antioxidant, α-amylase and α-glucosidase inhibitory activities of dried methanol ex tract from the bark of this tree. The plant material was collected in eastern Serbia (Jelašnička Klisura gorge). In the dried bark, the contents of different classes of phenolics were spectrophotometrically determined: total polyphenols (10.36%), tannins (8.78%), procyanidins (4.21%) and phenolic glycosides (3.54%). After pre-extraction with dichloromethane, powdered bark was extracted with methanol by bimaceration at room temperature. In obtained dried methanol extract, using aforementioned spectrophotometric tests, the contents of total polyphenols (33.42%) and tannins (21.55%) were determined, and by HPLC, arbutin, chlorogenic acid, catechin and procyanidin B2 were identifi ed. Its antioxidant activity, i.e. ferric reducing capacity (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radical scavenging ability, as well as the inhibition of the enzymes α-amylase and α-glucosidase were assessed using corresponding colorimetric assays. Tested dried methanol extract exhibited signifi cant DPPH and OH radical scavenging abilities (SC50 = 6.85 and 12.21 μg/mL, respectively), and ferric reducing capacity (10.74 mmol Fe (II)/g of dried extract), as well as the inhibition of α-amylase (IC50 = 11.4 μg/mL) and α-glucosidase (IC50 = 5.48 μg/mL).
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark
VL  - 42
IS  - 1 supplement
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5258
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Stanojković, Tatjana and Đorđić Crnogorac, Marija and Niketić, Marjan and Petrović, Silvana",
year = "2018",
abstract = "Pyrus × velenovskyi Dostálek (Rosaceae) [P. pyraster (L.) Burgsd. × P. spinosa Forssk.] is a deciduous tree up to 5 m height. Leaf-blades 2.2–4.8 × 1.3–2.7 cm, ± glabrate, ellip tic, gradually narrowed to a rather long petiole. Th is natural hybrid plant was described from Bulgaria, and has been also found in Serbia. The objective of this work was to investigate the phenolic profile, as well as in vitro antioxidant, α-amylase and α-glucosidase inhibitory activities of dried methanol ex tract from the bark of this tree. The plant material was collected in eastern Serbia (Jelašnička Klisura gorge). In the dried bark, the contents of different classes of phenolics were spectrophotometrically determined: total polyphenols (10.36%), tannins (8.78%), procyanidins (4.21%) and phenolic glycosides (3.54%). After pre-extraction with dichloromethane, powdered bark was extracted with methanol by bimaceration at room temperature. In obtained dried methanol extract, using aforementioned spectrophotometric tests, the contents of total polyphenols (33.42%) and tannins (21.55%) were determined, and by HPLC, arbutin, chlorogenic acid, catechin and procyanidin B2 were identifi ed. Its antioxidant activity, i.e. ferric reducing capacity (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radical scavenging ability, as well as the inhibition of the enzymes α-amylase and α-glucosidase were assessed using corresponding colorimetric assays. Tested dried methanol extract exhibited signifi cant DPPH and OH radical scavenging abilities (SC50 = 6.85 and 12.21 μg/mL, respectively), and ferric reducing capacity (10.74 mmol Fe (II)/g of dried extract), as well as the inhibition of α-amylase (IC50 = 11.4 μg/mL) and α-glucosidase (IC50 = 5.48 μg/mL).",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark",
volume = "42",
number = "1 supplement",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5258"
}

Milutinović, V., Ušjak, L., Stanojković, T., Đorđić Crnogorac, M., Niketić, M.,& Petrović, S.. (2018). Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 139-139.
https://hdl.handle.net/21.15107/rcub_farfar_5258

Milutinović V, Ušjak L, Stanojković T, Đorđić Crnogorac M, Niketić M, Petrović S. Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):139-139.
https://hdl.handle.net/21.15107/rcub_farfar_5258 .

Milutinović, Violeta, Ušjak, Ljuboš, Stanojković, Tatjana, Đorđić Crnogorac, Marija, Niketić, Marjan, Petrović, Silvana, "Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):139-139,
https://hdl.handle.net/21.15107/rcub_farfar_5258 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Petrović, Silvana
AU  - Niketić, Marjan
AU  - Krunić, Aleksej
AU  - Nikolić, Dejan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5260
AB  - The genus Hieracium L. is autochthonous in Eurasia, North Africa and North America and the
vast majority of the taxa are distributed in Europe. Hieracium naegelianum Pančić, belonging
to monotypic subendemic section H. sect. Naegeliana Zahn ex Szeląg, is a small, caespitose
plant, with long rhizome. It inhabits subalpine and alpine orosubmediterranean screes and
mainly is endemic for the mountains in W Balkan, with a single population in S Apennines.
Hieracium scheppigianum Freyn is a hybridogenous apomictic species probably originated
from H. gymnocephalum Griseb. ex Pant. (H. sect. Pannosa /Zahn (Zahn) and H. bupleuroides
C.C. Gmelin, s. l. (H. sect. Drepanoidea Monnier). The species can be found only on screes,
rocky places or rocky pastures in subalpine and alpine zone in Dinarides. The underground
parts of H. naegelianum and H. scheppigianum were collected on Mt Durmitor, Republic of
Montenegro. Dried and powered plant material was macerated with dichloromethane and
methanol, successively. Both solvents were evaporated under reduced pressure and dried
methanol extracts were used for further investigation. Using LC-MS method, sesquiterpene
lactone glycoside of guaianolide type, crepiside E, and six phenolic acids, i.e. chlorogenic acid
and five dicaffeoylquinic acids (cynarin, 3,5-, 1,5-, 3,4- and 4,5-dicaffeoylquinic acids) were
identified and quantified in both dried methanol extracts. Crepiside E was the most abundant
compound (116.58 and 126.88 mg/g), while among phenolic acids, 3,5-dicaffeoylquinic
acid (72.86 and 62.01 mg/g) was dominant in both investigated extracts. Radical scavenging
activity was estimated using colorimetric DPPH and OH radical assays, and both extracts
showed prominent and concentration dependent activity with SC50DPPH of 25.25 and
29.38 μg/mL and SC50OH of 16.09 and 17.50 μg/mL, respectively. This is the first report on
secondary metabolites and antiradical potential of underground parts of H. naegelianum and
H. scheppigianum
PB  - University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
PB  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Zagreb, Croatia
C3  - 10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts
T1  - Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts
SP  - 113
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5260
ER  - 

@conference{
author = "Milutinović, Violeta and Petrović, Silvana and Niketić, Marjan and Krunić, Aleksej and Nikolić, Dejan",
year = "2018",
abstract = "The genus Hieracium L. is autochthonous in Eurasia, North Africa and North America and the
vast majority of the taxa are distributed in Europe. Hieracium naegelianum Pančić, belonging
to monotypic subendemic section H. sect. Naegeliana Zahn ex Szeląg, is a small, caespitose
plant, with long rhizome. It inhabits subalpine and alpine orosubmediterranean screes and
mainly is endemic for the mountains in W Balkan, with a single population in S Apennines.
Hieracium scheppigianum Freyn is a hybridogenous apomictic species probably originated
from H. gymnocephalum Griseb. ex Pant. (H. sect. Pannosa /Zahn (Zahn) and H. bupleuroides
C.C. Gmelin, s. l. (H. sect. Drepanoidea Monnier). The species can be found only on screes,
rocky places or rocky pastures in subalpine and alpine zone in Dinarides. The underground
parts of H. naegelianum and H. scheppigianum were collected on Mt Durmitor, Republic of
Montenegro. Dried and powered plant material was macerated with dichloromethane and
methanol, successively. Both solvents were evaporated under reduced pressure and dried
methanol extracts were used for further investigation. Using LC-MS method, sesquiterpene
lactone glycoside of guaianolide type, crepiside E, and six phenolic acids, i.e. chlorogenic acid
and five dicaffeoylquinic acids (cynarin, 3,5-, 1,5-, 3,4- and 4,5-dicaffeoylquinic acids) were
identified and quantified in both dried methanol extracts. Crepiside E was the most abundant
compound (116.58 and 126.88 mg/g), while among phenolic acids, 3,5-dicaffeoylquinic
acid (72.86 and 62.01 mg/g) was dominant in both investigated extracts. Radical scavenging
activity was estimated using colorimetric DPPH and OH radical assays, and both extracts
showed prominent and concentration dependent activity with SC50DPPH of 25.25 and
29.38 μg/mL and SC50OH of 16.09 and 17.50 μg/mL, respectively. This is the first report on
secondary metabolites and antiradical potential of underground parts of H. naegelianum and
H. scheppigianum",
publisher = "University of Zagreb, Faculty of Agriculture, Zagreb, Croatia, Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Zagreb, Croatia",
journal = "10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts",
title = "Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5260"
}

Milutinović, V., Petrović, S., Niketić, M., Krunić, A.,& Nikolić, D.. (2018). Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts. in 10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts
University of Zagreb, Faculty of Agriculture, Zagreb, Croatia., 113-113.
https://hdl.handle.net/21.15107/rcub_farfar_5260

Milutinović V, Petrović S, Niketić M, Krunić A, Nikolić D. Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts. in 10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts. 2018;:113-113.
https://hdl.handle.net/21.15107/rcub_farfar_5260 .

Milutinović, Violeta, Petrović, Silvana, Niketić, Marjan, Krunić, Aleksej, Nikolić, Dejan, "Phytochemical analysis and antiradical potential of methanol extracts of Hieracium naegelianum Pančić and H. scheppigianum Freyn underground parts" in 10th Conference on Medicinal and Aromatic Plants of Southeast European Countries (10th CMAPSEEC): Book of Abstracts (2018):113-113,
https://hdl.handle.net/21.15107/rcub_farfar_5260 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petković, Miloš
AU  - Petrović, Silvana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5237
AB  - Rod Hieracium L. s. str. (Asteraceae) predstavlja jedan od najbrojnijih i
najkompleksnijih rodova skrivenosemenica. Na Balkanskom poluostrvu, najveći broj
vrsta ovog roda zabeležen je na Dinaridima, naročito na Durmitoru u Crnoj Gori. Cilj
ovog istraživanja bio je da se utvrdi i međusobno uporedi profil triterpenskih jedinjenja
u dihlormetanskim ekstraktima nadzemnih delova u cvetu 28 vrsta ovog roda poreklom iz
Crne Gore i Srbije, i to: H. gymnocephalum Griseb. ex Pant., H. orieni A. Kern., H. blecicii
Niketić, H. paratrichum Niketić, H. spirocaule Niketić, H. mokragorae (Nägeli & Peter)
Nägeli & Peter, H. pannosum Boiss. s.l., H. plumulosum A. Kern., H. villosum Jacq., H.
pilosum Froel., H. pseudoschenkii (Rohlena & Zahn) Niketić, H. naegelianum Pančić, H.
anastrum (Degen & Zahn) Niketić, H. calophyllum R. Uechtr., H. scheppigianum Freyn, H.
durmitoricum (Rohlena & Zahn) Niketić, H. guentheri‐beckii Zahn, H. mirificissimum
Rohlena & Zahn, H. coloriscapum Rohlena & Zahn, H. pyricephalum Niketić, H.
albopellitum (Zahn) Niketić, H. glabratum Willd., H. scorzonerifolium Vill. s.l., H. dentatum
Hoppe s.l., H. valdepilosum Vill. s.l., H. neilreichii Beck, H. tommasinianum K. Malý i H.
macrodontoides (Zahn) Zahn.
U suvim dihlormetanskim ekstraktima nadzemnih delova biljaka u cvetu vršena
je komparativna kvalitativna i kvantitativna GC‐FID‐MS analiza triterpena. Struktura
triterpena utvrđena je poređenjem sa komercijalnim standardima, kao i na osnovu 1D i
2D NMR spektara odgovarajućih frakcija.
U ispitivanim vrstama identifikovano je ukupno pet triterpena, i to dva derivata
ursana (α‐amirin i njegov acetat), dva derivata oleana (β‐amirin i njegov acetat) i jedan
derivat lupana (lupeol‐acetat). Dominantni triterpeni, u većini ispitivanih vrsta, bili su
derivati ursana, a praćeni su jedinjenjima tipa oleana.
U ovom radu je, za većinu (26) odabranih biljnih vrsta, ispitivanje triterpena
sprovedeno po prvi put. Na osnovu dobijenih rezultata, značajno su upotpunjeni podaci
o prisustvu i kompoziciji triterpenskih jedinjenja u vrstama roda Hieracium
AB  - The genus Hieracium L. s. str. (Asteraceae) represents one of the most numerous
and most complex genera of flowering plants. In the Balkan Peninsula the largest
number of species of this genus are recorded on Mt Durmitor in Montenegro. The
objective of this study was to determine and compare triterpene profile of flowering
aerial parts dichloromethane extracts of 28 Hieracium species from Montenegro and
Serbia: H. gymnocephalum Griseb. ex Pant., H. orieni A. Kern., H. blecicii Niketić, H.
paratrichum Niketić, H. spirocaule Niketić, H. mokragorae (Nägeli & Peter) Nägeli &
Peter, H. pannosum Boiss. s.l., H. plumulosum A. Kern., H. villosum Jacq., H. pilosum Froel.,
H. pseudoschenkii (Rohlena & Zahn) Niketić, H. naegelianum Pančić, H. anastrum (Degen
& Zahn) Niketić, H. calophyllum R. Uechtr., H. scheppigianum Freyn, H. durmitoricum
(Rohlena & Zahn) Niketić, H. guentheri‐beckii Zahn, H. mirificissimum Rohlena & Zahn, H.
coloriscapum Rohlena & Zahn, H. pyricephalum Niketić, H. albopellitum (Zahn) Niketić, H.
glabratum Willd., H. scorzonerifolium Vill. s.l., H. dentatum Hoppe s.l., H. valdepilosum Vill.
s.l., H. neilreichii Beck, H. tommasinianum K. Malý and H. macrodontoides (Zahn) Zahn.
In dried dichloromethane extracts of flowering aerial plants parts comparative
qualitative and quantitative GC‐FID‐MS analysis of triterpenes was performed. The
structures of triterpenes were elucidated comparing to commercial standards, and on
the basis of 1D and 2D NMR spectra of corresponding fractions.
In investigated species, overall five triterpenes, including two of ursane
(α‐amyrin and its acetate), two of oleane (β‐amyrin and its acetate) and one of lupane
(lupeol acetate) type, were identified. Dominant triterpenes, for the majority of species,
were of ursane type, followed by representatives of oleane type.
In this paper, for the majority (26) of selected plant species, triterpenes were
tested for the first time. Based on obtained results, the data on the presence and
composition of triterpen compounds in Hieracium species have been significantly
supplemented.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Ispitivanje triterpena u dihlormetanskim ekstraktima 28 vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva
T1  - Investigation of triterpenes in the dichloromethane extracts of 28 Hieracium L. species (Asteraceae) from the Balkan peninsula
VL  - 68
IS  - 3
SP  - 714
EP  - 715
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5237
ER  - 

@conference{
author = "Milutinović, Violeta and Niketić, Marjan and Petković, Miloš and Petrović, Silvana",
year = "2018",
abstract = "Rod Hieracium L. s. str. (Asteraceae) predstavlja jedan od najbrojnijih i
najkompleksnijih rodova skrivenosemenica. Na Balkanskom poluostrvu, najveći broj
vrsta ovog roda zabeležen je na Dinaridima, naročito na Durmitoru u Crnoj Gori. Cilj
ovog istraživanja bio je da se utvrdi i međusobno uporedi profil triterpenskih jedinjenja
u dihlormetanskim ekstraktima nadzemnih delova u cvetu 28 vrsta ovog roda poreklom iz
Crne Gore i Srbije, i to: H. gymnocephalum Griseb. ex Pant., H. orieni A. Kern., H. blecicii
Niketić, H. paratrichum Niketić, H. spirocaule Niketić, H. mokragorae (Nägeli & Peter)
Nägeli & Peter, H. pannosum Boiss. s.l., H. plumulosum A. Kern., H. villosum Jacq., H.
pilosum Froel., H. pseudoschenkii (Rohlena & Zahn) Niketić, H. naegelianum Pančić, H.
anastrum (Degen & Zahn) Niketić, H. calophyllum R. Uechtr., H. scheppigianum Freyn, H.
durmitoricum (Rohlena & Zahn) Niketić, H. guentheri‐beckii Zahn, H. mirificissimum
Rohlena & Zahn, H. coloriscapum Rohlena & Zahn, H. pyricephalum Niketić, H.
albopellitum (Zahn) Niketić, H. glabratum Willd., H. scorzonerifolium Vill. s.l., H. dentatum
Hoppe s.l., H. valdepilosum Vill. s.l., H. neilreichii Beck, H. tommasinianum K. Malý i H.
macrodontoides (Zahn) Zahn.
U suvim dihlormetanskim ekstraktima nadzemnih delova biljaka u cvetu vršena
je komparativna kvalitativna i kvantitativna GC‐FID‐MS analiza triterpena. Struktura
triterpena utvrđena je poređenjem sa komercijalnim standardima, kao i na osnovu 1D i
2D NMR spektara odgovarajućih frakcija.
U ispitivanim vrstama identifikovano je ukupno pet triterpena, i to dva derivata
ursana (α‐amirin i njegov acetat), dva derivata oleana (β‐amirin i njegov acetat) i jedan
derivat lupana (lupeol‐acetat). Dominantni triterpeni, u većini ispitivanih vrsta, bili su
derivati ursana, a praćeni su jedinjenjima tipa oleana.
U ovom radu je, za većinu (26) odabranih biljnih vrsta, ispitivanje triterpena
sprovedeno po prvi put. Na osnovu dobijenih rezultata, značajno su upotpunjeni podaci
o prisustvu i kompoziciji triterpenskih jedinjenja u vrstama roda Hieracium, The genus Hieracium L. s. str. (Asteraceae) represents one of the most numerous
and most complex genera of flowering plants. In the Balkan Peninsula the largest
number of species of this genus are recorded on Mt Durmitor in Montenegro. The
objective of this study was to determine and compare triterpene profile of flowering
aerial parts dichloromethane extracts of 28 Hieracium species from Montenegro and
Serbia: H. gymnocephalum Griseb. ex Pant., H. orieni A. Kern., H. blecicii Niketić, H.
paratrichum Niketić, H. spirocaule Niketić, H. mokragorae (Nägeli & Peter) Nägeli &
Peter, H. pannosum Boiss. s.l., H. plumulosum A. Kern., H. villosum Jacq., H. pilosum Froel.,
H. pseudoschenkii (Rohlena & Zahn) Niketić, H. naegelianum Pančić, H. anastrum (Degen
& Zahn) Niketić, H. calophyllum R. Uechtr., H. scheppigianum Freyn, H. durmitoricum
(Rohlena & Zahn) Niketić, H. guentheri‐beckii Zahn, H. mirificissimum Rohlena & Zahn, H.
coloriscapum Rohlena & Zahn, H. pyricephalum Niketić, H. albopellitum (Zahn) Niketić, H.
glabratum Willd., H. scorzonerifolium Vill. s.l., H. dentatum Hoppe s.l., H. valdepilosum Vill.
s.l., H. neilreichii Beck, H. tommasinianum K. Malý and H. macrodontoides (Zahn) Zahn.
In dried dichloromethane extracts of flowering aerial plants parts comparative
qualitative and quantitative GC‐FID‐MS analysis of triterpenes was performed. The
structures of triterpenes were elucidated comparing to commercial standards, and on
the basis of 1D and 2D NMR spectra of corresponding fractions.
In investigated species, overall five triterpenes, including two of ursane
(α‐amyrin and its acetate), two of oleane (β‐amyrin and its acetate) and one of lupane
(lupeol acetate) type, were identified. Dominant triterpenes, for the majority of species,
were of ursane type, followed by representatives of oleane type.
In this paper, for the majority (26) of selected plant species, triterpenes were
tested for the first time. Based on obtained results, the data on the presence and
composition of triterpen compounds in Hieracium species have been significantly
supplemented.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Ispitivanje triterpena u dihlormetanskim ekstraktima 28 vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva, Investigation of triterpenes in the dichloromethane extracts of 28 Hieracium L. species (Asteraceae) from the Balkan peninsula",
volume = "68",
number = "3",
pages = "714-715",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5237"
}

Milutinović, V., Niketić, M., Petković, M.,& Petrović, S.. (2018). Ispitivanje triterpena u dihlormetanskim ekstraktima 28 vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 714-715.
https://hdl.handle.net/21.15107/rcub_farfar_5237

Milutinović V, Niketić M, Petković M, Petrović S. Ispitivanje triterpena u dihlormetanskim ekstraktima 28 vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Arhiv za farmaciju. 2018;68(3):714-715.
https://hdl.handle.net/21.15107/rcub_farfar_5237 .

Milutinović, Violeta, Niketić, Marjan, Petković, Miloš, Petrović, Silvana, "Ispitivanje triterpena u dihlormetanskim ekstraktima 28 vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva" in Arhiv za farmaciju, 68, no. 3 (2018):714-715,
https://hdl.handle.net/21.15107/rcub_farfar_5237 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5236
AB  - Cytisus jankae Velen. (Fabaceae) is a dwarfish shrub (10–25
cm height) with 3-foliolate leaves and capitate inflorescence
with tubular calyces. The whole plant is densely covered with
silky hairs. Its range includes central and east part of the Bal-
kan Peninsula and Romania. It inhabits dry rocky places on
limestone or ultramafic soils. The objective of this study was
to establish the phytochemical profile of flowering aerial parts
of this plant. The plant material was collected in eastern Ser-
bia (Jelašnička Klisura gorge). In the air-dried herb, by the
direct gravimetric method, the total alkaloids content (0.32%)
was determined, and using colorimetric assays the total poly-
phenols (43.8%), tannins (1.13%), and the total flavonoids
(1.31%) were quantified. Additionally, the powdered dried
plant material was successively extracted with dichlorometh-
ane (maceration) and methanol (bimaceration) at room tem-
perature. After the solvents were evaporated under reduced
pressure, dried extracts were subjected to the further analy-
sis. The total alkaloids content in both the dichloromethane
(7.01%) and methanol (3.47%) extracts, and the contents of
total polyphenols (13.75%), tannins (2.45%) and flavonoids
(4.65%) in the methanol extract, were determined by afore-
mentioned tests. Applying the LC-MS method, in the metha-
nol extract flavonoids quercetin 3-O-rutinoside, luteolin, api-
genin and genistein were identified. GC-MS analysis resulted
in the identification of quinolizidine alkaloids sparteine,
17-oxosparteine and lupanine (2-oxosparteine) in both the
methanol and dichloromethane extracts, as well as of one tri-
terpene, β-amyrin, in the dichloromethane extract. This is the
first report on specialised metabolites from flowering aerial
parts of C. jankae.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part
VL  - 42
IS  - 1 supplement
SP  - 143
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5236
ER  - 

@conference{
author = "Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2018",
abstract = "Cytisus jankae Velen. (Fabaceae) is a dwarfish shrub (10–25
cm height) with 3-foliolate leaves and capitate inflorescence
with tubular calyces. The whole plant is densely covered with
silky hairs. Its range includes central and east part of the Bal-
kan Peninsula and Romania. It inhabits dry rocky places on
limestone or ultramafic soils. The objective of this study was
to establish the phytochemical profile of flowering aerial parts
of this plant. The plant material was collected in eastern Ser-
bia (Jelašnička Klisura gorge). In the air-dried herb, by the
direct gravimetric method, the total alkaloids content (0.32%)
was determined, and using colorimetric assays the total poly-
phenols (43.8%), tannins (1.13%), and the total flavonoids
(1.31%) were quantified. Additionally, the powdered dried
plant material was successively extracted with dichlorometh-
ane (maceration) and methanol (bimaceration) at room tem-
perature. After the solvents were evaporated under reduced
pressure, dried extracts were subjected to the further analy-
sis. The total alkaloids content in both the dichloromethane
(7.01%) and methanol (3.47%) extracts, and the contents of
total polyphenols (13.75%), tannins (2.45%) and flavonoids
(4.65%) in the methanol extract, were determined by afore-
mentioned tests. Applying the LC-MS method, in the metha-
nol extract flavonoids quercetin 3-O-rutinoside, luteolin, api-
genin and genistein were identified. GC-MS analysis resulted
in the identification of quinolizidine alkaloids sparteine,
17-oxosparteine and lupanine (2-oxosparteine) in both the
methanol and dichloromethane extracts, as well as of one tri-
terpene, β-amyrin, in the dichloromethane extract. This is the
first report on specialised metabolites from flowering aerial
parts of C. jankae.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part",
volume = "42",
number = "1 supplement",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5236"
}

Milutinović, V., Niketić, M.,& Petrović, S.. (2018). Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 143-143.
https://hdl.handle.net/21.15107/rcub_farfar_5236

Milutinović V, Niketić M, Petrović S. Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):143-143.
https://hdl.handle.net/21.15107/rcub_farfar_5236 .

Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):143-143,
https://hdl.handle.net/21.15107/rcub_farfar_5236 .