TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4469
AB  - Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.
PB  - Taylor and Francis Group
T2  - Journal of Essential Oil Research
T1  - Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils
VL  - 35
IS  - 2
SP  - 154
EP  - 167
DO  - 10.1080/10412905.2022.2147675
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.",
publisher = "Taylor and Francis Group",
journal = "Journal of Essential Oil Research",
title = "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils",
volume = "35",
number = "2",
pages = "154-167",
doi = "10.1080/10412905.2022.2147675"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2023). Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research
Taylor and Francis Group., 35(2), 154-167.
https://doi.org/10.1080/10412905.2022.2147675

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research. 2023;35(2):154-167.
doi:10.1080/10412905.2022.2147675 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils" in Journal of Essential Oil Research, 35, no. 2 (2023):154-167,
https://doi.org/10.1080/10412905.2022.2147675 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4276
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Elsevier Ltd., 402.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023),
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - JOUR
AU  - Gopčević, Kristina
AU  - Grujić, Slavica
AU  - Arsenijević, Jelena
AU  - Džamić, Ana
AU  - Veličković, Ivona
AU  - Izrael-Živković, Lidija
AU  - Medić, Ana
AU  - Mudrić, Jelena
AU  - Soković, Marina
AU  - Đurić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4342
AB  - The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.
PB  - Nature Research
T2  - Scientific Reports
T1  - Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction
VL  - 12
IS  - 1
DO  - 10.1038/s41598-022-25668-3
ER  - 

@article{
author = "Gopčević, Kristina and Grujić, Slavica and Arsenijević, Jelena and Džamić, Ana and Veličković, Ivona and Izrael-Živković, Lidija and Medić, Ana and Mudrić, Jelena and Soković, Marina and Đurić, Ana",
year = "2022",
abstract = "The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction",
volume = "12",
number = "1",
doi = "10.1038/s41598-022-25668-3"
}

Gopčević, K., Grujić, S., Arsenijević, J., Džamić, A., Veličković, I., Izrael-Živković, L., Medić, A., Mudrić, J., Soković, M.,& Đurić, A.. (2022). Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports
Nature Research., 12(1).
https://doi.org/10.1038/s41598-022-25668-3

Gopčević K, Grujić S, Arsenijević J, Džamić A, Veličković I, Izrael-Živković L, Medić A, Mudrić J, Soković M, Đurić A. Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports. 2022;12(1).
doi:10.1038/s41598-022-25668-3 .

Gopčević, Kristina, Grujić, Slavica, Arsenijević, Jelena, Džamić, Ana, Veličković, Ivona, Izrael-Živković, Lidija, Medić, Ana, Mudrić, Jelena, Soković, Marina, Đurić, Ana, "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction" in Scientific Reports, 12, no. 1 (2022),
https://doi.org/10.1038/s41598-022-25668-3 . .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana D.
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4172
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones
VL  - 19
IS  - 7
SP  - e202200326
DO  - 10.1002/cbdv.202200326
ER  - 

@article{
author = "Milutinović, Violeta and Matić, Ivana Z. and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana D. and Ušjak, Ljuboš and Niketić, Marjan S. and Petrović, Silvana",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones",
volume = "19",
number = "7",
pages = "e202200326",
doi = "10.1002/cbdv.202200326"
}

Milutinović, V., Matić, I. Z., Stanojković, T., Soković, M., Ćirić, A. D., Ušjak, L., Niketić, M. S.,& Petrović, S.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović V, Matić IZ, Stanojković T, Soković M, Ćirić AD, Ušjak L, Niketić MS, Petrović S. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta, Matić, Ivana Z., Stanojković, Tatjana, Soković, Marina, Ćirić, Ana D., Ušjak, Ljuboš, Niketić, Marjan S., Petrović, Silvana, "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones" in Chemistry and Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4502
AB  - In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives.
AB  - Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.
T1  - Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.
VL  - 72
IS  - 4 suplement
SP  - 455
EP  - 456
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4502
ER  - 

@conference{
author = "Ušjak, Ljuboš and Stojković, Dejan and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives., Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l., Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.",
volume = "72",
number = "4 suplement",
pages = "455-456",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4502"
}

Ušjak, L., Stojković, D., Soković, M., Niketić, M.,& Petrović, S.. (2022). Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), 455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502

Ušjak L, Stojković D, Soković M, Niketić M, Petrović S. Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju. 2022;72(4 suplement):455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

Ušjak, Ljuboš, Stojković, Dejan, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l." in Arhiv za farmaciju, 72, no. 4 suplement (2022):455-456,
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

TY  - JOUR
AU  - Čilović-Kozarević, Ermina
AU  - Šarić-Kundalić, Broza
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5107
AB  - Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.
PB  - Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg
VL  - 41
SP  - 35
EP  - 40
DO  - 10.5937/leksir2141035C
ER  - 

@article{
author = "Čilović-Kozarević, Ermina and Šarić-Kundalić, Broza and Ibišević, Merima and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2021",
abstract = "Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.",
publisher = "Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg",
volume = "41",
pages = "35-40",
doi = "10.5937/leksir2141035C"
}

Čilović-Kozarević, E., Šarić-Kundalić, B., Ibišević, M., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2021). GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd., 41, 35-40.
https://doi.org/10.5937/leksir2141035C

Čilović-Kozarević E, Šarić-Kundalić B, Ibišević M, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine. 2021;41:35-40.
doi:10.5937/leksir2141035C .

Čilović-Kozarević, Ermina, Šarić-Kundalić, Broza, Ibišević, Merima, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg" in Lekovite sirovine, 41 (2021):35-40,
https://doi.org/10.5937/leksir2141035C . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4615
AB  - INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.
PB  - University Prince of Songkla, Thailand
PB  - University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
T1  - In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies
SP  - 188
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4615
ER  - 

@conference{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2021",
abstract = "INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.",
publisher = "University Prince of Songkla, Thailand, University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts",
title = "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies",
pages = "188-189",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4615"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2021). In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
University Prince of Songkla, Thailand., 188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts. 2021;:188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts (2021):188-189,
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Matić, Ivana
AU  - Grozdanić-Stanisavljević, Nađa
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5109
AB  - Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones
SP  - 162
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5109
ER  - 

@conference{
author = "Milutinović, Violeta and Matić, Ivana and Grozdanić-Stanisavljević, Nađa and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones",
pages = "162-163",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5109"
}

Milutinović, V., Matić, I., Grozdanić-Stanisavljević, N., Stanojković, T., Soković, M., Ćirić, A., Niketić, M.,& Petrović, S.. (2019). Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109

Milutinović V, Matić I, Grozdanić-Stanisavljević N, Stanojković T, Soković M, Ćirić A, Niketić M, Petrović S. Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

Milutinović, Violeta, Matić, Ivana, Grozdanić-Stanisavljević, Nađa, Stanojković, Tatjana, Soković, Marina, Ćirić, Ana, Niketić, Marjan, Petrović, Silvana, "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):162-163,
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

TY  - GEN
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3482
AB  - Uvod: Predmet istraživanja je devet taksona roda Heracleum (Apiaceae): sedam iz grupe 
H. sphondylium (H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum), i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.
Rezultati i diskusija: Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja (ukupno 59 uzoraka) izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatični estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatični estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. Antimikrobna aktivnost 30 odabranih uzoraka etarskih ulja ispitivana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u slučaju 17 uzoraka ulja MIK vrednosti su prema barem jednom mikroorganizmu bile niže ili bliske 100 µg/mL); selektivna citotoksičnost 26 uzoraka ulja MTT testom na tumorske HeLa, LS174 i A549 ćelije (u slučaju 13 uzoraka ulja IC50 vrednosti su prema barem jednoj ćelijskoj liniji bile niže ili bliske 30 µg/mL; ulja uglavnom nisu inhibirala rast normalnih MRC-5 ćelija). Ukupno 8 od ispitivanih 14 uzoraka ulja pokazalo je slab anti-DPPH potencijal. Sastav aktivnih zona u TLC-DPPH testu ispitan je gasnom hromatografijom (dominirali su oksidovani terpeni i fenilpropani). S obzirom da je u pojedinim uljima utvrđeno prisustvo furanokumarina koji mogu delovati fototoksično, definisan je bezbednosni profil ulja prema preporuci Evropske agencije za lekove (utvrđeno je da dnevni unos 1,94-15,68 mL ulja ne doprinosi značajno ukupnom riziku, koji postoji kao posledica unosa izvesnih količina furanokumarina hranom).
U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin. U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni. 
Hemosistematski značaj komponenti etarskih ulja (u slučaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrđen je multivarijantnom statističkom analizom (PCA, nMDS i UPGMA).
Zaključak: Sprovedena hemijska karakterizacija značajno je doprinela boljem poznavanju kompozicije sekundarnih i primarnih metabolita odabranih predstavnika sekcije Heracleum, dok su ispitivanja H. austriacum subsp. siifolium iz sekcije Wendia sprovedena po prvi put. Utvrđeno je da su ispitivani taksoni potencijalno novi biljni izvori jedinjenja značajnih za farmaceutsku, kozmetičku, prehrambenu ili hemijsku industriju. Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na opravdanost daljih istraživanja ovih izolata kao potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.
T1  - Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe
T1  - Hemijska i farmakološka karakterizacija 9 taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3482
ER  - 

@misc{
author = "Ušjak, Ljuboš and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Uvod: Predmet istraživanja je devet taksona roda Heracleum (Apiaceae): sedam iz grupe 
H. sphondylium (H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum), i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.
Rezultati i diskusija: Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja (ukupno 59 uzoraka) izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatični estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatični estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. Antimikrobna aktivnost 30 odabranih uzoraka etarskih ulja ispitivana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u slučaju 17 uzoraka ulja MIK vrednosti su prema barem jednom mikroorganizmu bile niže ili bliske 100 µg/mL); selektivna citotoksičnost 26 uzoraka ulja MTT testom na tumorske HeLa, LS174 i A549 ćelije (u slučaju 13 uzoraka ulja IC50 vrednosti su prema barem jednoj ćelijskoj liniji bile niže ili bliske 30 µg/mL; ulja uglavnom nisu inhibirala rast normalnih MRC-5 ćelija). Ukupno 8 od ispitivanih 14 uzoraka ulja pokazalo je slab anti-DPPH potencijal. Sastav aktivnih zona u TLC-DPPH testu ispitan je gasnom hromatografijom (dominirali su oksidovani terpeni i fenilpropani). S obzirom da je u pojedinim uljima utvrđeno prisustvo furanokumarina koji mogu delovati fototoksično, definisan je bezbednosni profil ulja prema preporuci Evropske agencije za lekove (utvrđeno je da dnevni unos 1,94-15,68 mL ulja ne doprinosi značajno ukupnom riziku, koji postoji kao posledica unosa izvesnih količina furanokumarina hranom).
U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin. U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni. 
Hemosistematski značaj komponenti etarskih ulja (u slučaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrđen je multivarijantnom statističkom analizom (PCA, nMDS i UPGMA).
Zaključak: Sprovedena hemijska karakterizacija značajno je doprinela boljem poznavanju kompozicije sekundarnih i primarnih metabolita odabranih predstavnika sekcije Heracleum, dok su ispitivanja H. austriacum subsp. siifolium iz sekcije Wendia sprovedena po prvi put. Utvrđeno je da su ispitivani taksoni potencijalno novi biljni izvori jedinjenja značajnih za farmaceutsku, kozmetičku, prehrambenu ili hemijsku industriju. Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na opravdanost daljih istraživanja ovih izolata kao potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.",
title = "Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe, Hemijska i farmakološka karakterizacija 9 taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3482"
}

Ušjak, L., Drobac, M., Soković, M., Stanojković, T., Niketić, M.,& Petrović, S.. (2019). Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe. .
https://hdl.handle.net/21.15107/rcub_farfar_3482

Ušjak L, Drobac M, Soković M, Stanojković T, Niketić M, Petrović S. Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_3482 .

Ušjak, Ljuboš, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Niketić, Marjan, Petrović, Silvana, "Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe" (2019),
https://hdl.handle.net/21.15107/rcub_farfar_3482 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3000
AB  - Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC-MS. Monoterpenes prevailed in the root oil, with beta-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49-14.56 µg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 µg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils
VL  - 54
IS  - 8
SP  - 2193
EP  - 2202
DO  - 10.1007/s13197-017-2610-z
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2017",
abstract = "Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC-MS. Monoterpenes prevailed in the root oil, with beta-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49-14.56 µg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 µg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils",
volume = "54",
number = "8",
pages = "2193-2202",
doi = "10.1007/s13197-017-2610-z"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2017). Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 54(8), 2193-2202.
https://doi.org/10.1007/s13197-017-2610-z

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology-Mysore. 2017;54(8):2193-2202.
doi:10.1007/s13197-017-2610-z .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils" in Journal of Food Science and Technology-Mysore, 54, no. 8 (2017):2193-2202,
https://doi.org/10.1007/s13197-017-2610-z . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2908
AB  - Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils.
AB  - 
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants
VL  - 8
IS  - 1
SP  - 278
EP  - 290
DO  - 10.1039/c6fo01698g
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2017",
abstract = "Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils., ",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants",
volume = "8",
number = "1",
pages = "278-290",
doi = "10.1039/c6fo01698g"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2017). Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function
Royal Soc Chemistry, Cambridge., 8(1), 278-290.
https://doi.org/10.1039/c6fo01698g

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function. 2017;8(1):278-290.
doi:10.1039/c6fo01698g .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants" in Food & Function, 8, no. 1 (2017):278-290,
https://doi.org/10.1039/c6fo01698g . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2571
AB  - The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum
(Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene
(35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main
constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial
screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root
oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best
antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs
0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL)
and LS174 (IC50 24.16-58.86 μg/mL) cell lines.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis
VL  - 11
IS  - 4
SP  - 529
EP  - 534
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2990
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2016",
abstract = "The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum
(Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene
(35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main
constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial
screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root
oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best
antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs
0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL)
and LS174 (IC50 24.16-58.86 μg/mL) cell lines.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis",
volume = "11",
number = "4",
pages = "529-534",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2990"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2016). Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications
Natural Products Inc, Westerville., 11(4), 529-534.
https://hdl.handle.net/21.15107/rcub_ibiss_2990

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications. 2016;11(4):529-534.
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis" in Natural Product Communications, 11, no. 4 (2016):529-534,
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Grozdanić, Nađa
AU  - Niketić, Marjan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2534
AB  - In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančic and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50> 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50= 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50= 6.7 μg/ml).
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils
VL  - 13
IS  - 4
SP  - 466
EP  - 476
DO  - 10.1002/cbdv.201500151
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Grozdanić, Nađa and Niketić, Marjan",
year = "2016",
abstract = "In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančic and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50> 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50= 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50= 6.7 μg/ml).",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils",
volume = "13",
number = "4",
pages = "466-476",
doi = "10.1002/cbdv.201500151"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D., Grozdanić, N.,& Niketić, M.. (2016). Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 13(4), 466-476.
https://doi.org/10.1002/cbdv.201500151

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Grozdanić N, Niketić M. Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils. in Chemistry & Biodiversity. 2016;13(4):466-476.
doi:10.1002/cbdv.201500151 .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Grozdanić, Nađa, Niketić, Marjan, "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils" in Chemistry & Biodiversity, 13, no. 4 (2016):466-476,
https://doi.org/10.1002/cbdv.201500151 . .

TY  - JOUR
AU  - Popović, Višnja
AU  - Stojković, Dejan
AU  - Nikolić, Miloš
AU  - Heyerick, Arne
AU  - Petrović, Silvana
AU  - Soković, Marina
AU  - Niketić, Marjan
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2422
AB  - Antimicrobial properties of extracts of underground parts of three Laserpitium L. (Apiaceae) species, namely Laserpitium latifolium L., Laserpitium zernyi Hayek and Laserpitium ochridanum Micevski, were investigated. The investigated species are widely used as functional foods, as spices and for preparations in traditional medicine for treating complaints connected with infection and inflammation. Furthermore, antimicrobial and antibiofilm effects of laserpitine, the most abundant compound in the chloroform extract of Laserpitium latifolium, and guaianolide sesquiterpene lactones, such as, isomontanolide, montanolide and tarolide, principal components of the extracts of Laserpitium zernyi and Laserpitium ochridanum were assessed. The antimicrobial activity was tested using the microdilution method against five pathogenic bacteria and five fungi, as well as in the microplate biofilm assay on two Candida clinical isolates (C. albicans and C. krusei). Among the extracts, Laserpitium latifolium showed the most prominent activity. Isolated metabolites exerted higher effects against fungal than against bacterial strains, isomontanolide being the most active. Interestingly, all constituents showed higher potential on inhibition of biofilm formation than fluconazole, a reference compound. Tested metabolites may be good novel agents with high antifungal and antibacterial potential that might find practical applications in food industry as food preservatives in order to retard the growth of food spoiling microbes, but only after detailed safety assessments.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates
VL  - 6
IS  - 4
SP  - 1205
EP  - 1211
DO  - 10.1039/c5fo00066a
ER  - 

@article{
author = "Popović, Višnja and Stojković, Dejan and Nikolić, Miloš and Heyerick, Arne and Petrović, Silvana and Soković, Marina and Niketić, Marjan",
year = "2015",
abstract = "Antimicrobial properties of extracts of underground parts of three Laserpitium L. (Apiaceae) species, namely Laserpitium latifolium L., Laserpitium zernyi Hayek and Laserpitium ochridanum Micevski, were investigated. The investigated species are widely used as functional foods, as spices and for preparations in traditional medicine for treating complaints connected with infection and inflammation. Furthermore, antimicrobial and antibiofilm effects of laserpitine, the most abundant compound in the chloroform extract of Laserpitium latifolium, and guaianolide sesquiterpene lactones, such as, isomontanolide, montanolide and tarolide, principal components of the extracts of Laserpitium zernyi and Laserpitium ochridanum were assessed. The antimicrobial activity was tested using the microdilution method against five pathogenic bacteria and five fungi, as well as in the microplate biofilm assay on two Candida clinical isolates (C. albicans and C. krusei). Among the extracts, Laserpitium latifolium showed the most prominent activity. Isolated metabolites exerted higher effects against fungal than against bacterial strains, isomontanolide being the most active. Interestingly, all constituents showed higher potential on inhibition of biofilm formation than fluconazole, a reference compound. Tested metabolites may be good novel agents with high antifungal and antibacterial potential that might find practical applications in food industry as food preservatives in order to retard the growth of food spoiling microbes, but only after detailed safety assessments.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates",
volume = "6",
number = "4",
pages = "1205-1211",
doi = "10.1039/c5fo00066a"
}

Popović, V., Stojković, D., Nikolić, M., Heyerick, A., Petrović, S., Soković, M.,& Niketić, M.. (2015). Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates. in Food & Function
Royal Soc Chemistry, Cambridge., 6(4), 1205-1211.
https://doi.org/10.1039/c5fo00066a

Popović V, Stojković D, Nikolić M, Heyerick A, Petrović S, Soković M, Niketić M. Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates. in Food & Function. 2015;6(4):1205-1211.
doi:10.1039/c5fo00066a .

Popović, Višnja, Stojković, Dejan, Nikolić, Miloš, Heyerick, Arne, Petrović, Silvana, Soković, Marina, Niketić, Marjan, "Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates" in Food & Function, 6, no. 4 (2015):1205-1211,
https://doi.org/10.1039/c5fo00066a . .

TY  - JOUR
AU  - Hadžifejzović, Nihad
AU  - Kukić-Marković, Jelena
AU  - Petrović, Silvana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Nahrstedt, Adolf
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1866
AB  - Here we report on the antimicrobial and antioxidant activity of several extracts of Ruscus aculeatus L. and Ruscus hypoglossum L., as well as of some compounds previously isolated from them (p-coumaric and caffeic acids, rutin). For antimicrobial activity, the microdilution technique was used against eight bacterial and five fungal strains, along with standard drugs (streptomycin, ampicillin, bifonazole, ketoconazole). Investigated extracts and isolated compounds showed antibacterial and especially antifungal activity. In some cases this activity was better than standard drugs (streptomycin, ampicillin, bifonazole, ketoconazole) and was more evident for some of isolated compounds. Antioxidant activity was investigated by DPPH and 2-deoxyribose assays, along with spectrophotometrical determination of total phenolics content (TPC) using Folin-Ciocalteu reagent. In DPPH test obtained SC50 values were in a range of 173-541 mu g/mL, while inhibition of 2-deoxyribose for some extracts reached up to maximal 50%. Results of antioxidant assays were strongly correlated with total phenolics content. The most active was the EtOAc fraction of the MeOH extract of R. aculeatus herb regarding all investigated parameters.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.
VL  - 49
SP  - 407
EP  - 411
DO  - 10.1016/j.indcrop.2013.05.036
ER  - 

@article{
author = "Hadžifejzović, Nihad and Kukić-Marković, Jelena and Petrović, Silvana and Soković, Marina and Glamočlija, Jasmina and Stojković, Dejan and Nahrstedt, Adolf",
year = "2013",
abstract = "Here we report on the antimicrobial and antioxidant activity of several extracts of Ruscus aculeatus L. and Ruscus hypoglossum L., as well as of some compounds previously isolated from them (p-coumaric and caffeic acids, rutin). For antimicrobial activity, the microdilution technique was used against eight bacterial and five fungal strains, along with standard drugs (streptomycin, ampicillin, bifonazole, ketoconazole). Investigated extracts and isolated compounds showed antibacterial and especially antifungal activity. In some cases this activity was better than standard drugs (streptomycin, ampicillin, bifonazole, ketoconazole) and was more evident for some of isolated compounds. Antioxidant activity was investigated by DPPH and 2-deoxyribose assays, along with spectrophotometrical determination of total phenolics content (TPC) using Folin-Ciocalteu reagent. In DPPH test obtained SC50 values were in a range of 173-541 mu g/mL, while inhibition of 2-deoxyribose for some extracts reached up to maximal 50%. Results of antioxidant assays were strongly correlated with total phenolics content. The most active was the EtOAc fraction of the MeOH extract of R. aculeatus herb regarding all investigated parameters.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.",
volume = "49",
pages = "407-411",
doi = "10.1016/j.indcrop.2013.05.036"
}

Hadžifejzović, N., Kukić-Marković, J., Petrović, S., Soković, M., Glamočlija, J., Stojković, D.,& Nahrstedt, A.. (2013). Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 49, 407-411.
https://doi.org/10.1016/j.indcrop.2013.05.036

Hadžifejzović N, Kukić-Marković J, Petrović S, Soković M, Glamočlija J, Stojković D, Nahrstedt A. Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.. in Industrial Crops and Products. 2013;49:407-411.
doi:10.1016/j.indcrop.2013.05.036 .

Hadžifejzović, Nihad, Kukić-Marković, Jelena, Petrović, Silvana, Soković, Marina, Glamočlija, Jasmina, Stojković, Dejan, Nahrstedt, Adolf, "Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L." in Industrial Crops and Products, 49 (2013):407-411,
https://doi.org/10.1016/j.indcrop.2013.05.036 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Kukić-Marković, Jelena
AU  - Petrović, Silvana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1858
AB  - Background Three pure compounds that naturally occur in plants were of particular interest to our study regarding the possibility of using them as food preservatives: p-coumaric acid (found in peanuts, tomatoes, carrots, garlic, wine, vinegar, etc.), caffeic acid (found in argan oil, oats, wheat, rice and olive oil) and rutin (found in asparagus, citrus fruits, berries, apple, apricot, asparagus, beef and beer). In the following study we investigated in situ antioxidant and antimicrobial activities of three pure compounds, namely caffeic acid, p-coumaric acid and rutin, naturally occurring in plants. Results Two food systems were used in order to obtain information on how these compounds react in actual food systems rather than microbiological media. The results indicated good antioxidant activity in in situ food systems. For tested phenolic compounds it was further shown that they successively inhibited the development of the isolated food contaminant Staphylococcus aureus in chicken soup. Panelist found that organoleptic characteristics of chicken soup and pork meat improved after treatment with phenolics. Conclusion Our findings alone, along with the potential use of phenolic compounds that are widespread in nature, may imply their potential use as preservatives in the food industry.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems
VL  - 93
IS  - 13
SP  - 3205
EP  - 3208
DO  - 10.1002/jsfa.6156
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Soković, Marina and Glamočlija, Jasmina and Kukić-Marković, Jelena and Petrović, Silvana",
year = "2013",
abstract = "Background Three pure compounds that naturally occur in plants were of particular interest to our study regarding the possibility of using them as food preservatives: p-coumaric acid (found in peanuts, tomatoes, carrots, garlic, wine, vinegar, etc.), caffeic acid (found in argan oil, oats, wheat, rice and olive oil) and rutin (found in asparagus, citrus fruits, berries, apple, apricot, asparagus, beef and beer). In the following study we investigated in situ antioxidant and antimicrobial activities of three pure compounds, namely caffeic acid, p-coumaric acid and rutin, naturally occurring in plants. Results Two food systems were used in order to obtain information on how these compounds react in actual food systems rather than microbiological media. The results indicated good antioxidant activity in in situ food systems. For tested phenolic compounds it was further shown that they successively inhibited the development of the isolated food contaminant Staphylococcus aureus in chicken soup. Panelist found that organoleptic characteristics of chicken soup and pork meat improved after treatment with phenolics. Conclusion Our findings alone, along with the potential use of phenolic compounds that are widespread in nature, may imply their potential use as preservatives in the food industry.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems",
volume = "93",
number = "13",
pages = "3205-3208",
doi = "10.1002/jsfa.6156"
}

Stojković, D., Petrović, J., Soković, M., Glamočlija, J., Kukić-Marković, J.,& Petrović, S.. (2013). In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken., 93(13), 3205-3208.
https://doi.org/10.1002/jsfa.6156

Stojković D, Petrović J, Soković M, Glamočlija J, Kukić-Marković J, Petrović S. In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. in Journal of the Science of Food and Agriculture. 2013;93(13):3205-3208.
doi:10.1002/jsfa.6156 .

Stojković, Dejan, Petrović, Jovana, Soković, Marina, Glamočlija, Jasmina, Kukić-Marković, Jelena, Petrović, Silvana, "In situ antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems" in Journal of the Science of Food and Agriculture, 93, no. 13 (2013):3205-3208,
https://doi.org/10.1002/jsfa.6156 . .

TY  - JOUR
AU  - Stanojković, Tatjana
AU  - Kolundžija, Branka
AU  - Ćirić, Ana D.
AU  - Soković, Marina
AU  - Nikolić, Dejan
AU  - Kundaković, Tatjana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1975
AB  - Methanolic extract of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae) and its major compond rosmarinic acid possessed strong antimicrobial activity against tested bacteria and fungi, and antitumour activity against malignant cells, but without antiproliferative effect on healthy cell line MRC5. Minimal inhibitory concentration (MIC) of methanolic extract was ranged between 0.0125-1.25 mg/ml, while minimal bactericidal concentration (MBC) was of 0.2-5.0 mg/ml and fungicidal (MFC) was 0.15-2.5 mg/ml. The rosmarinic acid exhibited better antimicrobial activity then methanol extract. The most sensitive species to rosmarinic acid were Bacillus cereus and Candida kruzei, while Micrococcus flavus, Trichoderma viride and Aspergillus niger were the most resistant. The methanol extract exhibited strong activity against Fem-x human malignant melanoma cells with an IC50 39.66 +/- 2.71 mu g/ml, and moderate activity against other cancer cell lines (IC50 from 138.06 +/- 0.16 mu g/ml against estrogen-dependant breast cancer cell lines MDA-MB-361 to 173.15 +/- 0.02 against a human epithelial cervical cancer cells HeLa). Rosmarinic acid arrested G2/M phase cell cycle in Fem-x cells, against which both methanol extract and rosmarinic acid possessed the best cytotoxic activity.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Cytotoxicity and antimicrobial activity of satureja kitaibelii wierzb. Ex heuff (lamiaceae)
VL  - 8
IS  - 2
SP  - 845
EP  - 854
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1021
ER  - 

@article{
author = "Stanojković, Tatjana and Kolundžija, Branka and Ćirić, Ana D. and Soković, Marina and Nikolić, Dejan and Kundaković, Tatjana",
year = "2013",
abstract = "Methanolic extract of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae) and its major compond rosmarinic acid possessed strong antimicrobial activity against tested bacteria and fungi, and antitumour activity against malignant cells, but without antiproliferative effect on healthy cell line MRC5. Minimal inhibitory concentration (MIC) of methanolic extract was ranged between 0.0125-1.25 mg/ml, while minimal bactericidal concentration (MBC) was of 0.2-5.0 mg/ml and fungicidal (MFC) was 0.15-2.5 mg/ml. The rosmarinic acid exhibited better antimicrobial activity then methanol extract. The most sensitive species to rosmarinic acid were Bacillus cereus and Candida kruzei, while Micrococcus flavus, Trichoderma viride and Aspergillus niger were the most resistant. The methanol extract exhibited strong activity against Fem-x human malignant melanoma cells with an IC50 39.66 +/- 2.71 mu g/ml, and moderate activity against other cancer cell lines (IC50 from 138.06 +/- 0.16 mu g/ml against estrogen-dependant breast cancer cell lines MDA-MB-361 to 173.15 +/- 0.02 against a human epithelial cervical cancer cells HeLa). Rosmarinic acid arrested G2/M phase cell cycle in Fem-x cells, against which both methanol extract and rosmarinic acid possessed the best cytotoxic activity.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Cytotoxicity and antimicrobial activity of satureja kitaibelii wierzb. Ex heuff (lamiaceae)",
volume = "8",
number = "2",
pages = "845-854",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1021"
}

Stanojković, T., Kolundžija, B., Ćirić, A. D., Soković, M., Nikolić, D.,& Kundaković, T.. (2013). Cytotoxicity and antimicrobial activity of satureja kitaibelii wierzb. Ex heuff (lamiaceae). in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 8(2), 845-854.
https://hdl.handle.net/21.15107/rcub_ibiss_1021

Stanojković T, Kolundžija B, Ćirić AD, Soković M, Nikolić D, Kundaković T. Cytotoxicity and antimicrobial activity of satureja kitaibelii wierzb. Ex heuff (lamiaceae). in Digest Journal of Nanomaterials and Biostructures. 2013;8(2):845-854.
https://hdl.handle.net/21.15107/rcub_ibiss_1021 .

Stanojković, Tatjana, Kolundžija, Branka, Ćirić, Ana D., Soković, Marina, Nikolić, Dejan, Kundaković, Tatjana, "Cytotoxicity and antimicrobial activity of satureja kitaibelii wierzb. Ex heuff (lamiaceae)" in Digest Journal of Nanomaterials and Biostructures, 8, no. 2 (2013):845-854,
https://hdl.handle.net/21.15107/rcub_ibiss_1021 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ferreira, Isabel C. F. R.
AU  - Janković, Teodora
AU  - Maksimović, Zoran
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1935
AB  - This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system
VL  - 55
SP  - 209
EP  - 213
DO  - 10.1016/j.fct.2013.01.005
ER  - 

@article{
author = "Stojković, Dejan and Živković, Jelena and Soković, Marina and Glamočlija, Jasmina and Ferreira, Isabel C. F. R. and Janković, Teodora and Maksimović, Zoran",
year = "2013",
abstract = "This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system",
volume = "55",
pages = "209-213",
doi = "10.1016/j.fct.2013.01.005"
}

Stojković, D., Živković, J., Soković, M., Glamočlija, J., Ferreira, I. C. F. R., Janković, T.,& Maksimović, Z.. (2013). Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 55, 209-213.
https://doi.org/10.1016/j.fct.2013.01.005

Stojković D, Živković J, Soković M, Glamočlija J, Ferreira ICFR, Janković T, Maksimović Z. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology. 2013;55:209-213.
doi:10.1016/j.fct.2013.01.005 .

Stojković, Dejan, Živković, Jelena, Soković, Marina, Glamočlija, Jasmina, Ferreira, Isabel C. F. R., Janković, Teodora, Maksimović, Zoran, "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system" in Food and Chemical Toxicology, 55 (2013):209-213,
https://doi.org/10.1016/j.fct.2013.01.005 . .

TY  - JOUR
AU  - Kundaković, Tatjana
AU  - Ćirić, Ana D.
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Nikolić, Vesna D.
AU  - Nikolić, Goran
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1595
AB  - According to the tested antimicrobial activity of constituents and their chemical characteristics, a new formulation of oral antiseptic was made based on garlic bulb powder and its active principles with strong antimicrobial activity against a wide range of bacteria and fungi. The antimicrobial activity of garlic bulb powder, allicin and the lozenge with 15% of garlic powder was tested using broth microdilution method. The tested garlic powder, as well the lozenge, have shown very high antimicrobial activity with MIC 1.25-5.00 mg/ml, and 1.87-7.50 mg/ml, respectively. The major compound, allicin, was highly active at a very low concentration. The minimal inhibitory concentration of allicin was from 6.25-12.50 μg/ml for antibacterial activity and 0.4 μg/ml for antifungal activity. Those concentrations are comparable with concentrations of commercially available antibiotics and fungicides. The formulation of an antiseptic with herbal constituents, high antibacterial and antifungal activity and pleasant taste could be an alternative to classical pharmaceutical oral antiseptics.
AB  - Na osnovu ispitane antimikrobne aktivnosti komponenata i njihovih hemijskih karakteristika, napravljena je nova formulacija oralnog antiseptika koji kao aktivnu komponentu ima prašak belog luka čiji sastojci pokazuju veoma izraženu aktivnost protiv širokog spektra bakterija i gljive Candida albicans. Antimikrobna aktivnost praška belog luka, alicina i lozengi sa 15% praška belog luka je ispitivana bujon mikrodilucionom metodom. Testiran prašak belog luka, kao i lozenge imaju veoma jaku antimikrobnu aktivnost sa minimalnim inhibitornim koncentracijama (MIC) 1,25-5,00 mg/ml (prašak belog luka) i 1,87-7,50 mg/ml (lozenge). Glavna aktivna komponeta belog luka, alicin, pokazuje antimikrobnu aktivnost u veoma niskim koncentracijama. Minimalne inhibitorne koncentracije alicina se kreću od 6,25-12,5 μg/ml protiv bakterija i 0,4 μg/ml protiv gljivice Candida albicans. Ove koncentracije se mogu uporediti sa koncentracijama komercijalnih antibiotika i fungicida. Naši rezultati su u skladu sa prethodnim ispitivanjima alicina i različitih ekstrakta belog luka, i potvrđuju najvažniju ulogu alicina u antimikrobnoj aktivnosti. Veoma je važna visoka aktivnost ovako formulisanog oralnog preparata protiv gljivice C. albicans koja je čest uzročnik infekcija usne duplje. Formulisan proizvod sa antiseptičkim osobinama karakteriše stanje čvrstog rastvora gde su aktivne supstance molekularno-disperzno ugrađene u obliku dobro rastvornog polimernog matriksa (sorbitola) velike specifične površine. Dobra rastvorljivost polimernog matriksa i velika specifična površina omogućuju zadovoljavajuću brzinu otpuštanja aktivnih principa iz belog luka (alicin). Pored visoke antimikrobne aktivnosti, lozenge poseduju prijatan ukus i osvežavaju dah, pa se mogu koristiti kao prirodni antiseptik u prevenciji i terapiji infekcija sluzokože usta i ždrela. Formulacija antiseptika sa biljnim sastojcima i visokom antibakterijskom i antifungalnom aktivnošću može biti alternativa farmaceutskim oralnim antisepticima.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Antimicrobial activity of lozenge with garlic bulb powder
T1  - Antimikrobna aktivnost lozengi sa praškom belog luka
VL  - 65
IS  - 5
SP  - 607
EP  - 610
DO  - 10.2298/HEMIND110414048K
ER  - 

@article{
author = "Kundaković, Tatjana and Ćirić, Ana D. and Soković, Marina and Milenković, Marina and Nikolić, Vesna D. and Nikolić, Goran",
year = "2011",
abstract = "According to the tested antimicrobial activity of constituents and their chemical characteristics, a new formulation of oral antiseptic was made based on garlic bulb powder and its active principles with strong antimicrobial activity against a wide range of bacteria and fungi. The antimicrobial activity of garlic bulb powder, allicin and the lozenge with 15% of garlic powder was tested using broth microdilution method. The tested garlic powder, as well the lozenge, have shown very high antimicrobial activity with MIC 1.25-5.00 mg/ml, and 1.87-7.50 mg/ml, respectively. The major compound, allicin, was highly active at a very low concentration. The minimal inhibitory concentration of allicin was from 6.25-12.50 μg/ml for antibacterial activity and 0.4 μg/ml for antifungal activity. Those concentrations are comparable with concentrations of commercially available antibiotics and fungicides. The formulation of an antiseptic with herbal constituents, high antibacterial and antifungal activity and pleasant taste could be an alternative to classical pharmaceutical oral antiseptics., Na osnovu ispitane antimikrobne aktivnosti komponenata i njihovih hemijskih karakteristika, napravljena je nova formulacija oralnog antiseptika koji kao aktivnu komponentu ima prašak belog luka čiji sastojci pokazuju veoma izraženu aktivnost protiv širokog spektra bakterija i gljive Candida albicans. Antimikrobna aktivnost praška belog luka, alicina i lozengi sa 15% praška belog luka je ispitivana bujon mikrodilucionom metodom. Testiran prašak belog luka, kao i lozenge imaju veoma jaku antimikrobnu aktivnost sa minimalnim inhibitornim koncentracijama (MIC) 1,25-5,00 mg/ml (prašak belog luka) i 1,87-7,50 mg/ml (lozenge). Glavna aktivna komponeta belog luka, alicin, pokazuje antimikrobnu aktivnost u veoma niskim koncentracijama. Minimalne inhibitorne koncentracije alicina se kreću od 6,25-12,5 μg/ml protiv bakterija i 0,4 μg/ml protiv gljivice Candida albicans. Ove koncentracije se mogu uporediti sa koncentracijama komercijalnih antibiotika i fungicida. Naši rezultati su u skladu sa prethodnim ispitivanjima alicina i različitih ekstrakta belog luka, i potvrđuju najvažniju ulogu alicina u antimikrobnoj aktivnosti. Veoma je važna visoka aktivnost ovako formulisanog oralnog preparata protiv gljivice C. albicans koja je čest uzročnik infekcija usne duplje. Formulisan proizvod sa antiseptičkim osobinama karakteriše stanje čvrstog rastvora gde su aktivne supstance molekularno-disperzno ugrađene u obliku dobro rastvornog polimernog matriksa (sorbitola) velike specifične površine. Dobra rastvorljivost polimernog matriksa i velika specifična površina omogućuju zadovoljavajuću brzinu otpuštanja aktivnih principa iz belog luka (alicin). Pored visoke antimikrobne aktivnosti, lozenge poseduju prijatan ukus i osvežavaju dah, pa se mogu koristiti kao prirodni antiseptik u prevenciji i terapiji infekcija sluzokože usta i ždrela. Formulacija antiseptika sa biljnim sastojcima i visokom antibakterijskom i antifungalnom aktivnošću može biti alternativa farmaceutskim oralnim antisepticima.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Antimicrobial activity of lozenge with garlic bulb powder, Antimikrobna aktivnost lozengi sa praškom belog luka",
volume = "65",
number = "5",
pages = "607-610",
doi = "10.2298/HEMIND110414048K"
}

Kundaković, T., Ćirić, A. D., Soković, M., Milenković, M., Nikolić, V. D.,& Nikolić, G.. (2011). Antimicrobial activity of lozenge with garlic bulb powder. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 65(5), 607-610.
https://doi.org/10.2298/HEMIND110414048K

Kundaković T, Ćirić AD, Soković M, Milenković M, Nikolić VD, Nikolić G. Antimicrobial activity of lozenge with garlic bulb powder. in Hemijska industrija. 2011;65(5):607-610.
doi:10.2298/HEMIND110414048K .

Kundaković, Tatjana, Ćirić, Ana D., Soković, Marina, Milenković, Marina, Nikolić, Vesna D., Nikolić, Goran, "Antimicrobial activity of lozenge with garlic bulb powder" in Hemijska industrija, 65, no. 5 (2011):607-610,
https://doi.org/10.2298/HEMIND110414048K . .

TY  - CONF
AU  - Hadžifejzović, N.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Petrović, Silvana
AU  - Kukić-Marković, Jelena
AU  - Nahrstedt, Adolf
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1287
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Antimicrobial activity of selected extracts and some compounds from Ruscus aculeatus L., R. hypoglossum L. and R. alexandrinus Garsault (Ruscaceae)
VL  - 75
IS  - 9
SP  - 1039
EP  - 1039
DO  - 10.1055/s-0029-1234843
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1444
ER  - 

@conference{
author = "Hadžifejzović, N. and Soković, Marina and Glamočlija, Jasmina and Stojković, Dejan and Petrović, Silvana and Kukić-Marković, Jelena and Nahrstedt, Adolf",
year = "2009",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Antimicrobial activity of selected extracts and some compounds from Ruscus aculeatus L., R. hypoglossum L. and R. alexandrinus Garsault (Ruscaceae)",
volume = "75",
number = "9",
pages = "1039-1039",
doi = "10.1055/s-0029-1234843",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1444"
}

Hadžifejzović, N., Soković, M., Glamočlija, J., Stojković, D., Petrović, S., Kukić-Marković, J.,& Nahrstedt, A.. (2009). Antimicrobial activity of selected extracts and some compounds from Ruscus aculeatus L., R. hypoglossum L. and R. alexandrinus Garsault (Ruscaceae). in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 75(9), 1039-1039.
https://doi.org/10.1055/s-0029-1234843
https://hdl.handle.net/21.15107/rcub_ibiss_1444

Hadžifejzović N, Soković M, Glamočlija J, Stojković D, Petrović S, Kukić-Marković J, Nahrstedt A. Antimicrobial activity of selected extracts and some compounds from Ruscus aculeatus L., R. hypoglossum L. and R. alexandrinus Garsault (Ruscaceae). in Planta Medica. 2009;75(9):1039-1039.
doi:10.1055/s-0029-1234843
https://hdl.handle.net/21.15107/rcub_ibiss_1444 .

Hadžifejzović, N., Soković, Marina, Glamočlija, Jasmina, Stojković, Dejan, Petrović, Silvana, Kukić-Marković, Jelena, Nahrstedt, Adolf, "Antimicrobial activity of selected extracts and some compounds from Ruscus aculeatus L., R. hypoglossum L. and R. alexandrinus Garsault (Ruscaceae)" in Planta Medica, 75, no. 9 (2009):1039-1039,
https://doi.org/10.1055/s-0029-1234843 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1444 .

TY  - JOUR
AU  - Stojković, Slaviša
AU  - Petrović, Silvana
AU  - Kukić, Jelena
AU  - Džamić, Ana
AU  - Ristić, Mihailo
AU  - Milenković, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Stojković, Dejan
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1282
PB  - Springer, New York
T2  - Chemistry of Natural Compounds
T1  - Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil
VL  - 45
IS  - 2
SP  - 253
EP  - 256
DO  - 10.1007/s10600-009-9268-3
ER  - 

@article{
author = "Stojković, Slaviša and Petrović, Silvana and Kukić, Jelena and Džamić, Ana and Ristić, Mihailo and Milenković, Marina and Glamočlija, Jasmina and Soković, Marina and Stojković, Dejan",
year = "2009",
publisher = "Springer, New York",
journal = "Chemistry of Natural Compounds",
title = "Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil",
volume = "45",
number = "2",
pages = "253-256",
doi = "10.1007/s10600-009-9268-3"
}

Stojković, S., Petrović, S., Kukić, J., Džamić, A., Ristić, M., Milenković, M., Glamočlija, J., Soković, M.,& Stojković, D.. (2009). Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil. in Chemistry of Natural Compounds
Springer, New York., 45(2), 253-256.
https://doi.org/10.1007/s10600-009-9268-3

Stojković S, Petrović S, Kukić J, Džamić A, Ristić M, Milenković M, Glamočlija J, Soković M, Stojković D. Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil. in Chemistry of Natural Compounds. 2009;45(2):253-256.
doi:10.1007/s10600-009-9268-3 .

Stojković, Slaviša, Petrović, Silvana, Kukić, Jelena, Džamić, Ana, Ristić, Mihailo, Milenković, Marina, Glamočlija, Jasmina, Soković, Marina, Stojković, Dejan, "Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil" in Chemistry of Natural Compounds, 45, no. 2 (2009):253-256,
https://doi.org/10.1007/s10600-009-9268-3 . .

TY  - CONF
AU  - Ivković, Branka
AU  - Soković, Marina
AU  - Marković, Bojan
AU  - Vladimirova, S.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1195
PB  - Medimond S R L, 40128 Bologna
C3  - Joint Meeting on Medical Chemistry
T1  - Synthesis and Evaluation of Derivatives of Phenylpropiophenone as potential antibacterial and antifungal agents
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1195
ER  - 

@conference{
author = "Ivković, Branka and Soković, Marina and Marković, Bojan and Vladimirova, S.",
year = "2009",
publisher = "Medimond S R L, 40128 Bologna",
journal = "Joint Meeting on Medical Chemistry",
title = "Synthesis and Evaluation of Derivatives of Phenylpropiophenone as potential antibacterial and antifungal agents",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1195"
}

Ivković, B., Soković, M., Marković, B.,& Vladimirova, S.. (2009). Synthesis and Evaluation of Derivatives of Phenylpropiophenone as potential antibacterial and antifungal agents. in Joint Meeting on Medical Chemistry
Medimond S R L, 40128 Bologna., 61.
https://hdl.handle.net/21.15107/rcub_farfar_1195

Ivković B, Soković M, Marković B, Vladimirova S. Synthesis and Evaluation of Derivatives of Phenylpropiophenone as potential antibacterial and antifungal agents. in Joint Meeting on Medical Chemistry. 2009;:61.
https://hdl.handle.net/21.15107/rcub_farfar_1195 .

Ivković, Branka, Soković, Marina, Marković, Bojan, Vladimirova, S., "Synthesis and Evaluation of Derivatives of Phenylpropiophenone as potential antibacterial and antifungal agents" in Joint Meeting on Medical Chemistry (2009):61,
https://hdl.handle.net/21.15107/rcub_farfar_1195 .

TY  - JOUR
AU  - Kukić, Jelena
AU  - Popović, Višnja
AU  - Petrović, Silvana
AU  - Mucaji, Pavel
AU  - Ćirić, Ana D.
AU  - Stojković, Dejan
AU  - Soković, Marina
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3548
AB  - The whole, fresh involucral bracts of cardoon, Cynara cardunculus L. (Compositae), were extracted with EtOH and an aqueous suspension of the obtained EtOH extract was partitioned successively with CHCl3, EtOAc and n-BuOH, leaving a residual water extract. All obtained extracts were evaluated for their antioxidant and antimicrobial properties. The antioxidant potential was evaluated using following in vitro methods: FRAP (ferric reducing antioxidant power) assay, and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Antimicrobial activity was estimated using a microdilution technique against food-borne, mycotoxin producers and human pathogenic bacteria and micromycetes. The following bacteria were tested: Salmonella typhimurium, Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, as well as micromycetes: Aspergillus niger, Aspergillus ochraceus, Aspergillus flavus, Penicillium ochrochloron, Penicillium funiculosum, Trichoderma viride, Fusarium tricinctum and Alternaria alternata. Results showed that all extracts possessed concentration-dependent antioxidant activity. In biological assays, C. cardunculus extracts showed antimicrobial activity comparable with standard antibiotics.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Antioxidant and antimicrobial activity of Cynara cardunculus extracts
VL  - 107
IS  - 2
SP  - 861
EP  - 868
DO  - 10.1016/j.foodchem.2007.09.005
ER  - 

@article{
author = "Kukić, Jelena and Popović, Višnja and Petrović, Silvana and Mucaji, Pavel and Ćirić, Ana D. and Stojković, Dejan and Soković, Marina",
year = "2008",
abstract = "The whole, fresh involucral bracts of cardoon, Cynara cardunculus L. (Compositae), were extracted with EtOH and an aqueous suspension of the obtained EtOH extract was partitioned successively with CHCl3, EtOAc and n-BuOH, leaving a residual water extract. All obtained extracts were evaluated for their antioxidant and antimicrobial properties. The antioxidant potential was evaluated using following in vitro methods: FRAP (ferric reducing antioxidant power) assay, and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Antimicrobial activity was estimated using a microdilution technique against food-borne, mycotoxin producers and human pathogenic bacteria and micromycetes. The following bacteria were tested: Salmonella typhimurium, Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, as well as micromycetes: Aspergillus niger, Aspergillus ochraceus, Aspergillus flavus, Penicillium ochrochloron, Penicillium funiculosum, Trichoderma viride, Fusarium tricinctum and Alternaria alternata. Results showed that all extracts possessed concentration-dependent antioxidant activity. In biological assays, C. cardunculus extracts showed antimicrobial activity comparable with standard antibiotics.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Antioxidant and antimicrobial activity of Cynara cardunculus extracts",
volume = "107",
number = "2",
pages = "861-868",
doi = "10.1016/j.foodchem.2007.09.005"
}

Kukić, J., Popović, V., Petrović, S., Mucaji, P., Ćirić, A. D., Stojković, D.,& Soković, M.. (2008). Antioxidant and antimicrobial activity of Cynara cardunculus extracts. in Food Chemistry
Elsevier Sci Ltd, Oxford., 107(2), 861-868.
https://doi.org/10.1016/j.foodchem.2007.09.005

Kukić J, Popović V, Petrović S, Mucaji P, Ćirić AD, Stojković D, Soković M. Antioxidant and antimicrobial activity of Cynara cardunculus extracts. in Food Chemistry. 2008;107(2):861-868.
doi:10.1016/j.foodchem.2007.09.005 .

Kukić, Jelena, Popović, Višnja, Petrović, Silvana, Mucaji, Pavel, Ćirić, Ana D., Stojković, Dejan, Soković, Marina, "Antioxidant and antimicrobial activity of Cynara cardunculus extracts" in Food Chemistry, 107, no. 2 (2008):861-868,
https://doi.org/10.1016/j.foodchem.2007.09.005 . .

TY  - JOUR
AU  - Kukić, Jelena
AU  - Popović, Višnja
AU  - Petrović, Silvana
AU  - Mucaji, Pavel
AU  - Ćirić, Ana D.
AU  - Stojković, Dejan
AU  - Soković, Marina
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1049
AB  - The whole, fresh involucral bracts of cardoon, Cynara cardunculus L. (Compositae), were extracted with EtOH and an aqueous suspension of the obtained EtOH extract was partitioned successively with CHCl3, EtOAc and n-BuOH, leaving a residual water extract. All obtained extracts were evaluated for their antioxidant and antimicrobial properties. The antioxidant potential was evaluated using following in vitro methods: FRAP (ferric reducing antioxidant power) assay, and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Antimicrobial activity was estimated using a microdilution technique against food-borne, mycotoxin producers and human pathogenic bacteria and micromycetes. The following bacteria were tested: Salmonella typhimurium, Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, as well as micromycetes: Aspergillus niger, Aspergillus ochraceus, Aspergillus flavus, Penicillium ochrochloron, Penicillium funiculosum, Trichoderma viride, Fusarium tricinctum and Alternaria alternata. Results showed that all extracts possessed concentration-dependent antioxidant activity. In biological assays, C. cardunculus extracts showed antimicrobial activity comparable with standard antibiotics.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Antioxidant and antimicrobial activity of Cynara cardunculus extracts
VL  - 107
IS  - 2
SP  - 861
EP  - 868
DO  - 10.1016/j.foodchem.2007.09.005
ER  - 

@article{
author = "Kukić, Jelena and Popović, Višnja and Petrović, Silvana and Mucaji, Pavel and Ćirić, Ana D. and Stojković, Dejan and Soković, Marina",
year = "2008",
abstract = "The whole, fresh involucral bracts of cardoon, Cynara cardunculus L. (Compositae), were extracted with EtOH and an aqueous suspension of the obtained EtOH extract was partitioned successively with CHCl3, EtOAc and n-BuOH, leaving a residual water extract. All obtained extracts were evaluated for their antioxidant and antimicrobial properties. The antioxidant potential was evaluated using following in vitro methods: FRAP (ferric reducing antioxidant power) assay, and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Antimicrobial activity was estimated using a microdilution technique against food-borne, mycotoxin producers and human pathogenic bacteria and micromycetes. The following bacteria were tested: Salmonella typhimurium, Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, as well as micromycetes: Aspergillus niger, Aspergillus ochraceus, Aspergillus flavus, Penicillium ochrochloron, Penicillium funiculosum, Trichoderma viride, Fusarium tricinctum and Alternaria alternata. Results showed that all extracts possessed concentration-dependent antioxidant activity. In biological assays, C. cardunculus extracts showed antimicrobial activity comparable with standard antibiotics.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Antioxidant and antimicrobial activity of Cynara cardunculus extracts",
volume = "107",
number = "2",
pages = "861-868",
doi = "10.1016/j.foodchem.2007.09.005"
}

Kukić, J., Popović, V., Petrović, S., Mucaji, P., Ćirić, A. D., Stojković, D.,& Soković, M.. (2008). Antioxidant and antimicrobial activity of Cynara cardunculus extracts. in Food Chemistry
Elsevier Sci Ltd, Oxford., 107(2), 861-868.
https://doi.org/10.1016/j.foodchem.2007.09.005

Kukić J, Popović V, Petrović S, Mucaji P, Ćirić AD, Stojković D, Soković M. Antioxidant and antimicrobial activity of Cynara cardunculus extracts. in Food Chemistry. 2008;107(2):861-868.
doi:10.1016/j.foodchem.2007.09.005 .

Kukić, Jelena, Popović, Višnja, Petrović, Silvana, Mucaji, Pavel, Ćirić, Ana D., Stojković, Dejan, Soković, Marina, "Antioxidant and antimicrobial activity of Cynara cardunculus extracts" in Food Chemistry, 107, no. 2 (2008):861-868,
https://doi.org/10.1016/j.foodchem.2007.09.005 . .