TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Klisić, Aleksandra
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Memon, Lidija
AU  - Gaković, Branka
AU  - Bogavac-Stanojević, Nataša
AU  - Spasojević-Kalimanovska, Vesna
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4677
AB  - Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.
PB  - Termedia Publishing House Ltd.
T2  - Archives of Medical Science
T1  - Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
VL  - 19
IS  - 2
SP  - 313
EP  - 323
DO  - 10.5114/aoms/136074
ER  - 

@article{
author = "Vukašinović, Aleksandra and Ostanek, Barbara and Klisić, Aleksandra and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Memon, Lidija and Gaković, Branka and Bogavac-Stanojević, Nataša and Spasojević-Kalimanovska, Vesna and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.",
publisher = "Termedia Publishing House Ltd.",
journal = "Archives of Medical Science",
title = "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress",
volume = "19",
number = "2",
pages = "313-323",
doi = "10.5114/aoms/136074"
}

Vukašinović, A., Ostanek, B., Klisić, A., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Memon, L., Gaković, B., Bogavac-Stanojević, N., Spasojević-Kalimanovska, V., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science
Termedia Publishing House Ltd.., 19(2), 313-323.
https://doi.org/10.5114/aoms/136074

Vukašinović A, Ostanek B, Klisić A, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Memon L, Gaković B, Bogavac-Stanojević N, Spasojević-Kalimanovska V, Marc J, Nešković A, Kotur-Stevuljević J. Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science. 2023;19(2):313-323.
doi:10.5114/aoms/136074 .

Vukašinović, Aleksandra, Ostanek, Barbara, Klisić, Aleksandra, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Memon, Lidija, Gaković, Branka, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress" in Archives of Medical Science, 19, no. 2 (2023):313-323,
https://doi.org/10.5114/aoms/136074 . .

TY  - JOUR
AU  - Kostadinović, Jelena
AU  - Popadić, Višeslav
AU  - Klašnja, Slobodan
AU  - Klisić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Andrić, Zoran
AU  - Zdravković, Marija
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4629
AB  - The clinical efficacy of chemotherapy, as a recognized therapeutic approach for malignant 
diseases,   usually   has   certain   limitations   due   to   its   cardiotoxicity   (CT)   and   consequent   
cardiomyopathy,  or  even  heart  failure.  CT
  is  defined  as  any  cardiac  injury  connected  with  
oncology treatment, whether it is chemo
-, radio
-, targeted or immunotherapy, or cancer by itself, 
and it represents a great challenge for clinicians in everyday practice. A wide spectrum of factors 
related t
o chemotherapy (type of drug, dose during each cycle, cumulative dose, schedule, method 
of  application,  combination  with  other  cardiotoxic  drugs  or  association  with  radiotherapy)  and  
patient  characteristics  (age,  presence  of  cardiovascular  risk  factors,  pr
evious  cardiovascular  
disease)  are  the  determining  factors  that  influence  the  frequency  of  CT.  Imaging  methods  for  
morphological  and  functional  monitoring  of  the  heart  muscle  are  used  for  monitoring  CT.  The  
quest  for  diagnostic  tools  for  early  CT  detection 
is  of  great  significance.  In  line  with  this,  the  
measurement  of  some  cardiac  biomarkers  has  found  its  place  in  clinical  settings  as  an  early  
determinant of myocardial injury. Therefore, in this review article, special attention will be paid 
to  certain  wel
l-established,  as  well  as  certain  novel  cardiac  biomarkers,  and  their  role  in  
recognizing asymptomatic CT, in order to gain deeper insight into their diagnostic utility.
AB  - Klinička  efikasnost  hemioterapije,  kao  priznatog  terapeutskog  pristupa  malignim 
bolestima, obično ima određena ograničenja zbog svoje kardiotoksičnosti (KT), te posledične 
kardiomiopatije, pa čak i srčane insuficijencije. KT se definiše kao svaka povreda miokarda 
povezana  s  lečenjem  raka,  uzimajući  u  obzir  hemioterapiju,  radioterapiju,  biološku  ili 
imunoterapiju,  kao  i  sam  karcinom,  te  predstavlja veliki  izazov  za  kliničare 
u  svakodnevnoj  
praksi. Širok spektar faktora povezanih s hemioterapijom (vrsta leka, doza tokom svakog ciklusa, 
kumulativna doza, raspored, način primene, kombinacija s drugim kardiotoksičnim lekovima ili 
povezanost s radioterapijom) i karakteristike bolesnika (dob, prisutnost faktora kardiovaskularnog 
rizika, prethodne kardiovaskularne bolesti), odlučujući su faktori koji utiču na učestalost KT. Za 
praćenje KT koriste se radiološke metode za morfološko i funkcionalno praćenje srčanog mišića. 
Potraga za dij
agnostičkim alatima za rano otkrivanje KT od velikog je značaja. U skladu s tim, 
merenje nekih srčanih biomarkera kao rane determinante povrede miokarda našlo je svoje mesto 
u kliničkim okruženjima. Stoga 
će u ovom preglednom članku posebna pažnja biti po
sve
ć
ena 
nekim  dobro  utvrđenim,  kao  i  nekim  novim  srčanim  biomarkerima,  i  njihovoj  ulozi  u 
prepoznavanju asimptomatske KT, kako bi se stekao dublji uvid u njihovu dijagnostičku korisnost 
i neželjene efekte lekova koji se primenjuju u terapiji karcinoma.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
T2  - Arhiv za farmaciju
T1  - Cardiotoxicity: Importance of biomarkers
T1  - Kardiotoksičnost: Važnost biomarkera
VL  - 73
IS  - 1
SP  - 1
EP  - 16
DO  - 10.5937/arhfarm73-40534
ER  - 

@article{
author = "Kostadinović, Jelena and Popadić, Višeslav and Klašnja, Slobodan and Klisić, Aleksandra and Kotur-Stevuljević, Jelena and Andrić, Zoran and Zdravković, Marija",
year = "2023",
abstract = "The clinical efficacy of chemotherapy, as a recognized therapeutic approach for malignant 
diseases,   usually   has   certain   limitations   due   to   its   cardiotoxicity   (CT)   and   consequent   
cardiomyopathy,  or  even  heart  failure.  CT
  is  defined  as  any  cardiac  injury  connected  with  
oncology treatment, whether it is chemo
-, radio
-, targeted or immunotherapy, or cancer by itself, 
and it represents a great challenge for clinicians in everyday practice. A wide spectrum of factors 
related t
o chemotherapy (type of drug, dose during each cycle, cumulative dose, schedule, method 
of  application,  combination  with  other  cardiotoxic  drugs  or  association  with  radiotherapy)  and  
patient  characteristics  (age,  presence  of  cardiovascular  risk  factors,  pr
evious  cardiovascular  
disease)  are  the  determining  factors  that  influence  the  frequency  of  CT.  Imaging  methods  for  
morphological  and  functional  monitoring  of  the  heart  muscle  are  used  for  monitoring  CT.  The  
quest  for  diagnostic  tools  for  early  CT  detection 
is  of  great  significance.  In  line  with  this,  the  
measurement  of  some  cardiac  biomarkers  has  found  its  place  in  clinical  settings  as  an  early  
determinant of myocardial injury. Therefore, in this review article, special attention will be paid 
to  certain  wel
l-established,  as  well  as  certain  novel  cardiac  biomarkers,  and  their  role  in  
recognizing asymptomatic CT, in order to gain deeper insight into their diagnostic utility., Klinička  efikasnost  hemioterapije,  kao  priznatog  terapeutskog  pristupa  malignim 
bolestima, obično ima određena ograničenja zbog svoje kardiotoksičnosti (KT), te posledične 
kardiomiopatije, pa čak i srčane insuficijencije. KT se definiše kao svaka povreda miokarda 
povezana  s  lečenjem  raka,  uzimajući  u  obzir  hemioterapiju,  radioterapiju,  biološku  ili 
imunoterapiju,  kao  i  sam  karcinom,  te  predstavlja veliki  izazov  za  kliničare 
u  svakodnevnoj  
praksi. Širok spektar faktora povezanih s hemioterapijom (vrsta leka, doza tokom svakog ciklusa, 
kumulativna doza, raspored, način primene, kombinacija s drugim kardiotoksičnim lekovima ili 
povezanost s radioterapijom) i karakteristike bolesnika (dob, prisutnost faktora kardiovaskularnog 
rizika, prethodne kardiovaskularne bolesti), odlučujući su faktori koji utiču na učestalost KT. Za 
praćenje KT koriste se radiološke metode za morfološko i funkcionalno praćenje srčanog mišića. 
Potraga za dij
agnostičkim alatima za rano otkrivanje KT od velikog je značaja. U skladu s tim, 
merenje nekih srčanih biomarkera kao rane determinante povrede miokarda našlo je svoje mesto 
u kliničkim okruženjima. Stoga 
će u ovom preglednom članku posebna pažnja biti po
sve
ć
ena 
nekim  dobro  utvrđenim,  kao  i  nekim  novim  srčanim  biomarkerima,  i  njihovoj  ulozi  u 
prepoznavanju asimptomatske KT, kako bi se stekao dublji uvid u njihovu dijagnostičku korisnost 
i neželjene efekte lekova koji se primenjuju u terapiji karcinoma.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Cardiotoxicity: Importance of biomarkers, Kardiotoksičnost: Važnost biomarkera",
volume = "73",
number = "1",
pages = "1-16",
doi = "10.5937/arhfarm73-40534"
}

Kostadinović, J., Popadić, V., Klašnja, S., Klisić, A., Kotur-Stevuljević, J., Andrić, Z.,& Zdravković, M.. (2023). Cardiotoxicity: Importance of biomarkers. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 73(1), 1-16.
https://doi.org/10.5937/arhfarm73-40534

Kostadinović J, Popadić V, Klašnja S, Klisić A, Kotur-Stevuljević J, Andrić Z, Zdravković M. Cardiotoxicity: Importance of biomarkers. in Arhiv za farmaciju. 2023;73(1):1-16.
doi:10.5937/arhfarm73-40534 .

Kostadinović, Jelena, Popadić, Višeslav, Klašnja, Slobodan, Klisić, Aleksandra, Kotur-Stevuljević, Jelena, Andrić, Zoran, Zdravković, Marija, "Cardiotoxicity: Importance of biomarkers" in Arhiv za farmaciju, 73, no. 1 (2023):1-16,
https://doi.org/10.5937/arhfarm73-40534 . .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Klisic, Aleksandra
AU  - Ostanek, Barbara
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Bogavac-Stanojević, Nataša
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5078
AB  - In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?
VL  - 24
IS  - 18
DO  - 10.3390/ijms241814308
ER  - 

@article{
author = "Vukašinović, Aleksandra and Klisic, Aleksandra and Ostanek, Barbara and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Bogavac-Stanojević, Nataša and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?",
volume = "24",
number = "18",
doi = "10.3390/ijms241814308"
}

Vukašinović, A., Klisic, A., Ostanek, B., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Bogavac-Stanojević, N., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences
MDPI., 24(18).
https://doi.org/10.3390/ijms241814308

Vukašinović A, Klisic A, Ostanek B, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Bogavac-Stanojević N, Marc J, Nešković A, Kotur-Stevuljević J. Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences. 2023;24(18).
doi:10.3390/ijms241814308 .

Vukašinović, Aleksandra, Klisic, Aleksandra, Ostanek, Barbara, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Bogavac-Stanojević, Nataša, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?" in International Journal of Molecular Sciences, 24, no. 18 (2023),
https://doi.org/10.3390/ijms241814308 . .

TY  - JOUR
AU  - Đekić, Saška
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Hinić, Saša
AU  - Cerović, Milivoje
AU  - Stefanović, Milica
AU  - Mihajlović, Marija
AU  - Nešković, Aleksandar
AU  - Bogavac-Stanojević, Nataša
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4843
AB  - The aim of this multicentric study was to assess the impacts of oxidative stress, inflam-
mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative
function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1)
activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In
69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using
polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each
HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The
distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within
HDL) was estimated using the zymogram method. The STEMI patients had significantly lower
proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1
within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013
and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive
associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and
pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased
proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small
HDL3 particles and the altered pPON1 within HDL.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction
VL  - 24
IS  - 11
DO  - 10.3390/ijms24119384
ER  - 

@article{
author = "Đekić, Saška and Vekić, Jelena and Zeljković, Aleksandra and Kotur-Stevuljević, Jelena and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Mihajlović, Marija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša",
year = "2023",
abstract = "The aim of this multicentric study was to assess the impacts of oxidative stress, inflam-
mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative
function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1)
activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In
69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using
polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each
HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The
distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within
HDL) was estimated using the zymogram method. The STEMI patients had significantly lower
proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1
within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013
and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive
associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and
pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased
proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small
HDL3 particles and the altered pPON1 within HDL.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction",
volume = "24",
number = "11",
doi = "10.3390/ijms24119384"
}

Đekić, S., Vekić, J., Zeljković, A., Kotur-Stevuljević, J., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Mihajlović, M., Nešković, A.,& Bogavac-Stanojević, N.. (2023). HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences
MDPI., 24(11).
https://doi.org/10.3390/ijms24119384

Đekić S, Vekić J, Zeljković A, Kotur-Stevuljević J, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Mihajlović M, Nešković A, Bogavac-Stanojević N. HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences. 2023;24(11).
doi:10.3390/ijms24119384 .

Đekić, Saška, Vekić, Jelena, Zeljković, Aleksandra, Kotur-Stevuljević, Jelena, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Mihajlović, Marija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction" in International Journal of Molecular Sciences, 24, no. 11 (2023),
https://doi.org/10.3390/ijms24119384 . .

TY  - JOUR
AU  - Rajkov, Branislava
AU  - Zdravković, Marija
AU  - Ninić, Ana
AU  - Brajković, Milica
AU  - Klašnja, Slobodan
AU  - Gardijan, Vera
AU  - Memon, Lidija
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Spasojević-Kalimanovska, Vesna
AU  - Radosavljević, Vojislav
AU  - Sopić, Miron
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4551
AB  - Purpose: Obstructive sleep apnea (OSA) is characterised by increased systemic inflammation, and is often accompanied with type 2 diabetes mellitus (T2DM) and cardiovascular disease. The aim of this investigation was to evaluate gene expression of resistin, its receptor CAP1 and CD36 as the indicators of the inflammatory changes in PBMCs in relation to the severity of OSA, and the presence of type 2 diabetes mellitus (T2DM) in OSA. Methods: Severity of OSA was defined by the apnea/hypopnea index (AHI): AHI < 30: mild to moderate OSA (MM-OSA), AHI ≥ 30: severe OSA (S-OSA). Presence of T2DM was captured: OSA with T2DM (OSA + T2DM), OSA without T2DM (OSA-T2DM). PBMC resistin, CAP1, and CD36 mRNA were determined by real-time PCR. Results: Resistin mRNA was significantly upregulated in S-OSA (N = 54) compared to the MM-OSA (N = 52, P = 0.043); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.302; P = 0.166, respectively). Resistin mRNA was significantly upregulated in OSA + T2DM (N = 29) compared to the OSA-T2DM (N = 77, P = 0.029); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.662; P = 0.108, respectively). AHI and T2DM were independent predictors of resistin mRNA above the 75th percentile (OR = 3.717 [1.152–11.991]; OR = 3.261 [1.000–10.630], P = 0.042 respectively). Conclusion: Resistin gene upregulation in S-OSA indicates its possible contribution to increased inflammation in S-OSA and makes it a possible marker of the disease severity. Resistin gene upregulation in OSA + T2DM suggests that a joint effect of these two comorbidities may have a major contribution to increased inflammation and complications that arise from this state.
PB  - Springer Nature
T2  - Sleep and Breathing
T1  - Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus
DO  - 10.1007/s11325-023-02809-0
ER  - 

@article{
author = "Rajkov, Branislava and Zdravković, Marija and Ninić, Ana and Brajković, Milica and Klašnja, Slobodan and Gardijan, Vera and Memon, Lidija and Munjas, Jelena and Mihajlović, Marija and Spasojević-Kalimanovska, Vesna and Radosavljević, Vojislav and Sopić, Miron",
year = "2023",
abstract = "Purpose: Obstructive sleep apnea (OSA) is characterised by increased systemic inflammation, and is often accompanied with type 2 diabetes mellitus (T2DM) and cardiovascular disease. The aim of this investigation was to evaluate gene expression of resistin, its receptor CAP1 and CD36 as the indicators of the inflammatory changes in PBMCs in relation to the severity of OSA, and the presence of type 2 diabetes mellitus (T2DM) in OSA. Methods: Severity of OSA was defined by the apnea/hypopnea index (AHI): AHI < 30: mild to moderate OSA (MM-OSA), AHI ≥ 30: severe OSA (S-OSA). Presence of T2DM was captured: OSA with T2DM (OSA + T2DM), OSA without T2DM (OSA-T2DM). PBMC resistin, CAP1, and CD36 mRNA were determined by real-time PCR. Results: Resistin mRNA was significantly upregulated in S-OSA (N = 54) compared to the MM-OSA (N = 52, P = 0.043); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.302; P = 0.166, respectively). Resistin mRNA was significantly upregulated in OSA + T2DM (N = 29) compared to the OSA-T2DM (N = 77, P = 0.029); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.662; P = 0.108, respectively). AHI and T2DM were independent predictors of resistin mRNA above the 75th percentile (OR = 3.717 [1.152–11.991]; OR = 3.261 [1.000–10.630], P = 0.042 respectively). Conclusion: Resistin gene upregulation in S-OSA indicates its possible contribution to increased inflammation in S-OSA and makes it a possible marker of the disease severity. Resistin gene upregulation in OSA + T2DM suggests that a joint effect of these two comorbidities may have a major contribution to increased inflammation and complications that arise from this state.",
publisher = "Springer Nature",
journal = "Sleep and Breathing",
title = "Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus",
doi = "10.1007/s11325-023-02809-0"
}

Rajkov, B., Zdravković, M., Ninić, A., Brajković, M., Klašnja, S., Gardijan, V., Memon, L., Munjas, J., Mihajlović, M., Spasojević-Kalimanovska, V., Radosavljević, V.,& Sopić, M.. (2023). Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus. in Sleep and Breathing
Springer Nature..
https://doi.org/10.1007/s11325-023-02809-0

Rajkov B, Zdravković M, Ninić A, Brajković M, Klašnja S, Gardijan V, Memon L, Munjas J, Mihajlović M, Spasojević-Kalimanovska V, Radosavljević V, Sopić M. Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus. in Sleep and Breathing. 2023;.
doi:10.1007/s11325-023-02809-0 .

Rajkov, Branislava, Zdravković, Marija, Ninić, Ana, Brajković, Milica, Klašnja, Slobodan, Gardijan, Vera, Memon, Lidija, Munjas, Jelena, Mihajlović, Marija, Spasojević-Kalimanovska, Vesna, Radosavljević, Vojislav, Sopić, Miron, "Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus" in Sleep and Breathing (2023),
https://doi.org/10.1007/s11325-023-02809-0 . .

TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanovich, Ljudmila
AU  - Đoković, Aleksandra
AU  - Todić, Brankica
AU  - Dopsaj, Violeta
AU  - Saponjski, Jovica
AU  - Saponjski, Dušan
AU  - Marković, Olivera
AU  - Belizna, Cristina
AU  - Zdravković, Marija
AU  - Marisavljević, Dragomir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4310
AB  - Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients
VL  - 23
IS  - 20
DO  - 10.3390/ijms232012309
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanovich, Ljudmila and Đoković, Aleksandra and Todić, Brankica and Dopsaj, Violeta and Saponjski, Jovica and Saponjski, Dušan and Marković, Olivera and Belizna, Cristina and Zdravković, Marija and Marisavljević, Dragomir",
year = "2022",
abstract = "Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients",
volume = "23",
number = "20",
doi = "10.3390/ijms232012309"
}

Stanisavljević, N., Stojanovich, L., Đoković, A., Todić, B., Dopsaj, V., Saponjski, J., Saponjski, D., Marković, O., Belizna, C., Zdravković, M.,& Marisavljević, D.. (2022). Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences
MDPI., 23(20).
https://doi.org/10.3390/ijms232012309

Stanisavljević N, Stojanovich L, Đoković A, Todić B, Dopsaj V, Saponjski J, Saponjski D, Marković O, Belizna C, Zdravković M, Marisavljević D. Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences. 2022;23(20).
doi:10.3390/ijms232012309 .

Stanisavljević, Nataša, Stojanovich, Ljudmila, Đoković, Aleksandra, Todić, Brankica, Dopsaj, Violeta, Saponjski, Jovica, Saponjski, Dušan, Marković, Olivera, Belizna, Cristina, Zdravković, Marija, Marisavljević, Dragomir, "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients" in International Journal of Molecular Sciences, 23, no. 20 (2022),
https://doi.org/10.3390/ijms232012309 . .

TY  - JOUR
AU  - Milojević, Ana
AU  - Zdravković, Marija
AU  - Brajković, Milica
AU  - Memon, Lidija
AU  - Gardijan, Vera
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Stefanović, Aleksandra
AU  - Mihajlović, Marija
AU  - Ivanišević, Jasmina
AU  - Bogavac-Stanojević, Nataša
AU  - Radosavljević, Vojislav
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ninić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4199
AB  - Objectives: Obstructive sleep apnea (OSA) is a common condition closely related to obesity, insulin resistance, dyslipidemia, and cardiovascular disease. The aim of this study was to explore the possible relationship between OSA and proprotein convertase subtilisin/kexin type 9 (PCSK9). Methods: Full-night polysomnography was performed on 150 participants who were divided into three groups: controls, OSA patients on statin therapy, and OSA patients not on statin therapy. Biochemical markers, plasma low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses, and PCSK9 were determined. Results: PCSK9 was highest in OSA patients on statins compared to the control group and to OSA patients not on statins (p = 0.036 and p = 0.039, respectively), after adjustment for body mass index (BMI). LDL diameter was greater in OSA patients not on statins compared to OSA patients on statins (p = 0.032). PCSK9 was highest in the group of patients with all three risk factors (diagnosed OSA, statins, BMI ≥25 kg/m2) compared to groups with no, one, and two risk factors (p = 0.031, p = 0.001, and p = 0.029, respectively). Presence of OSA, statin therapy, and BMI ≥25 kg/m2 when combined were independently associated with higher levels of PCSK9 when adjusted for antihypertensive therapy, small dense LDL, and HDL 3c subclass (odds ratio = 2.849; interquartile range [1.026–7.912], p = 0.044). Conclusion: Statin therapy was closely related to PCSK9. OSA along with obesity and statin use induces elevation of PCSK9.
PB  - S. Karger AG Basel
T2  - Medical Principles and Practice
T1  - Effects of Apnea, Obesity, and Statin Therapy on
Proprotein Convertase Subtilisin/Kexin 9 Levels
in Patients with Obstructive Sleep Apnea
VL  - 31
IS  - 3
SP  - 293
EP  - 300
DO  - 10.1159/000524087
ER  - 

@article{
author = "Milojević, Ana and Zdravković, Marija and Brajković, Milica and Memon, Lidija and Gardijan, Vera and Vekić, Jelena and Zeljković, Aleksandra and Stefanović, Aleksandra and Mihajlović, Marija and Ivanišević, Jasmina and Bogavac-Stanojević, Nataša and Radosavljević, Vojislav and Spasojević-Kalimanovska, Vesna and Ninić, Ana",
year = "2022",
abstract = "Objectives: Obstructive sleep apnea (OSA) is a common condition closely related to obesity, insulin resistance, dyslipidemia, and cardiovascular disease. The aim of this study was to explore the possible relationship between OSA and proprotein convertase subtilisin/kexin type 9 (PCSK9). Methods: Full-night polysomnography was performed on 150 participants who were divided into three groups: controls, OSA patients on statin therapy, and OSA patients not on statin therapy. Biochemical markers, plasma low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses, and PCSK9 were determined. Results: PCSK9 was highest in OSA patients on statins compared to the control group and to OSA patients not on statins (p = 0.036 and p = 0.039, respectively), after adjustment for body mass index (BMI). LDL diameter was greater in OSA patients not on statins compared to OSA patients on statins (p = 0.032). PCSK9 was highest in the group of patients with all three risk factors (diagnosed OSA, statins, BMI ≥25 kg/m2) compared to groups with no, one, and two risk factors (p = 0.031, p = 0.001, and p = 0.029, respectively). Presence of OSA, statin therapy, and BMI ≥25 kg/m2 when combined were independently associated with higher levels of PCSK9 when adjusted for antihypertensive therapy, small dense LDL, and HDL 3c subclass (odds ratio = 2.849; interquartile range [1.026–7.912], p = 0.044). Conclusion: Statin therapy was closely related to PCSK9. OSA along with obesity and statin use induces elevation of PCSK9.",
publisher = "S. Karger AG Basel",
journal = "Medical Principles and Practice",
title = "Effects of Apnea, Obesity, and Statin Therapy on
Proprotein Convertase Subtilisin/Kexin 9 Levels
in Patients with Obstructive Sleep Apnea",
volume = "31",
number = "3",
pages = "293-300",
doi = "10.1159/000524087"
}

Milojević, A., Zdravković, M., Brajković, M., Memon, L., Gardijan, V., Vekić, J., Zeljković, A., Stefanović, A., Mihajlović, M., Ivanišević, J., Bogavac-Stanojević, N., Radosavljević, V., Spasojević-Kalimanovska, V.,& Ninić, A.. (2022). Effects of Apnea, Obesity, and Statin Therapy on
Proprotein Convertase Subtilisin/Kexin 9 Levels
in Patients with Obstructive Sleep Apnea. in Medical Principles and Practice
S. Karger AG Basel., 31(3), 293-300.
https://doi.org/10.1159/000524087

Milojević A, Zdravković M, Brajković M, Memon L, Gardijan V, Vekić J, Zeljković A, Stefanović A, Mihajlović M, Ivanišević J, Bogavac-Stanojević N, Radosavljević V, Spasojević-Kalimanovska V, Ninić A. Effects of Apnea, Obesity, and Statin Therapy on
Proprotein Convertase Subtilisin/Kexin 9 Levels
in Patients with Obstructive Sleep Apnea. in Medical Principles and Practice. 2022;31(3):293-300.
doi:10.1159/000524087 .

Milojević, Ana, Zdravković, Marija, Brajković, Milica, Memon, Lidija, Gardijan, Vera, Vekić, Jelena, Zeljković, Aleksandra, Stefanović, Aleksandra, Mihajlović, Marija, Ivanišević, Jasmina, Bogavac-Stanojević, Nataša, Radosavljević, Vojislav, Spasojević-Kalimanovska, Vesna, Ninić, Ana, "Effects of Apnea, Obesity, and Statin Therapy on
Proprotein Convertase Subtilisin/Kexin 9 Levels
in Patients with Obstructive Sleep Apnea" in Medical Principles and Practice, 31, no. 3 (2022):293-300,
https://doi.org/10.1159/000524087 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Milojević, Ana
AU  - Vladimirov, Sandra
AU  - Zdravković, Marija
AU  - Memon, Lidija
AU  - Brajković, Milica
AU  - Gardijan, Vera
AU  - Gojković, Tamara
AU  - Stefanović, Aleksandra
AU  - Miljković-Trailović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ninić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4322
AB  - Background and aims: Obstructive sleep apnea (OSA) is closely linked to obesity and related adverse metabolic changes, including dyslipidemia. However, it is not clear whether OSA is an independent contributing factor to dyslipidemia, or the observed association is a reflection of a concomitant presence of obesity. Additionally, dyslipidemia is usually evaluated through measurement of parameters of routine lipid status, while more precise evaluation of lipid homeostasis is rarely performed in OSA. In this study, we analyzed markers of cholesterol synthesis and absorption in patients with OSA with respect to the presence of obesity and the disease severity. Methods and results: This study enrolled 116 OSA patients. Concentrations of non-cholesterol sterols (NCS), measured by LC-MS/MS, were used as markers of cholesterol synthesis and absorption. Apnea-hypopnea index (AHI) and oxygen saturation (SaO2) were utilized as markers of OSA severity. Serum lipid status parameters were determined by routine enzymatic methods. Markers of cholesterol synthesis were increased (P = 0.005), whilst markers of cholesterol absorption decreased (P = 0.001) in obese OSA patients. Cholesterol synthesis/absorption ratio was elevated in obese subjects (P < 0.001). Concentration of cholesterol synthesis marker lathosterol was significantly higher in subjects with severe OSA (P = 0.014) and we observed a trend of decreased cholesterol absorption in these patients. AHI was revealed as an independent determinant of lathosterol concentration (P = 0.022). Conclusions: Our results suggest that the presence of obesity and severe forms of OSA is characterized by elevated endogenous cholesterol synthesis. AHI was singled out as an independent determinant of the serum level of cholesterol synthesis marker lathosterol.
PB  - Elsevier B.V.
T2  - Nutrition, Metabolism and Cardiovascular Diseases
T1  - Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity
VL  - 32
IS  - 12
SP  - 2848
EP  - 2857
DO  - 10.1016/j.numecd.2022.09.006
ER  - 

@article{
author = "Zeljković, Aleksandra and Milojević, Ana and Vladimirov, Sandra and Zdravković, Marija and Memon, Lidija and Brajković, Milica and Gardijan, Vera and Gojković, Tamara and Stefanović, Aleksandra and Miljković-Trailović, Milica and Spasojević-Kalimanovska, Vesna and Ninić, Ana",
year = "2022",
abstract = "Background and aims: Obstructive sleep apnea (OSA) is closely linked to obesity and related adverse metabolic changes, including dyslipidemia. However, it is not clear whether OSA is an independent contributing factor to dyslipidemia, or the observed association is a reflection of a concomitant presence of obesity. Additionally, dyslipidemia is usually evaluated through measurement of parameters of routine lipid status, while more precise evaluation of lipid homeostasis is rarely performed in OSA. In this study, we analyzed markers of cholesterol synthesis and absorption in patients with OSA with respect to the presence of obesity and the disease severity. Methods and results: This study enrolled 116 OSA patients. Concentrations of non-cholesterol sterols (NCS), measured by LC-MS/MS, were used as markers of cholesterol synthesis and absorption. Apnea-hypopnea index (AHI) and oxygen saturation (SaO2) were utilized as markers of OSA severity. Serum lipid status parameters were determined by routine enzymatic methods. Markers of cholesterol synthesis were increased (P = 0.005), whilst markers of cholesterol absorption decreased (P = 0.001) in obese OSA patients. Cholesterol synthesis/absorption ratio was elevated in obese subjects (P < 0.001). Concentration of cholesterol synthesis marker lathosterol was significantly higher in subjects with severe OSA (P = 0.014) and we observed a trend of decreased cholesterol absorption in these patients. AHI was revealed as an independent determinant of lathosterol concentration (P = 0.022). Conclusions: Our results suggest that the presence of obesity and severe forms of OSA is characterized by elevated endogenous cholesterol synthesis. AHI was singled out as an independent determinant of the serum level of cholesterol synthesis marker lathosterol.",
publisher = "Elsevier B.V.",
journal = "Nutrition, Metabolism and Cardiovascular Diseases",
title = "Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity",
volume = "32",
number = "12",
pages = "2848-2857",
doi = "10.1016/j.numecd.2022.09.006"
}

Zeljković, A., Milojević, A., Vladimirov, S., Zdravković, M., Memon, L., Brajković, M., Gardijan, V., Gojković, T., Stefanović, A., Miljković-Trailović, M., Spasojević-Kalimanovska, V.,& Ninić, A.. (2022). Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity. in Nutrition, Metabolism and Cardiovascular Diseases
Elsevier B.V.., 32(12), 2848-2857.
https://doi.org/10.1016/j.numecd.2022.09.006

Zeljković A, Milojević A, Vladimirov S, Zdravković M, Memon L, Brajković M, Gardijan V, Gojković T, Stefanović A, Miljković-Trailović M, Spasojević-Kalimanovska V, Ninić A. Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity. in Nutrition, Metabolism and Cardiovascular Diseases. 2022;32(12):2848-2857.
doi:10.1016/j.numecd.2022.09.006 .

Zeljković, Aleksandra, Milojević, Ana, Vladimirov, Sandra, Zdravković, Marija, Memon, Lidija, Brajković, Milica, Gardijan, Vera, Gojković, Tamara, Stefanović, Aleksandra, Miljković-Trailović, Milica, Spasojević-Kalimanovska, Vesna, Ninić, Ana, "Alterations of cholesterol synthesis and absorption in obstructive sleep apnea: Influence of obesity and disease severity" in Nutrition, Metabolism and Cardiovascular Diseases, 32, no. 12 (2022):2848-2857,
https://doi.org/10.1016/j.numecd.2022.09.006 . .

TY  - CONF
AU  - Sopić, Miron
AU  - Šupljeglav, Branislava
AU  - Milojević, Ana
AU  - Mihajlović, Marija
AU  - Ninić, Ana
AU  - Munjas, Jelena
AU  - Gardijan, Vera
AU  - Radosavljević, Vojislav
AU  - Memon, Lidija
AU  - Zdravković, Marija
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5586
AB  - Background and Aims: Obstructive sleep apnea (OSA) is associated with increased risk of cardiovascular disease. Resistin is recognized as a potent proinflammatory cytokine that is able to influence atherosclerotic plaque progression through several mechanisms. ...
PB  - Elsevier
C3  - Atherosclerosis
T1  - Increased gene expression of resistin and CD36 in peripheral blood mononuclear cells is associated with hypercholesterolemia in patients with obstructive sleep apnea
VL  - 315
SP  - e48
EP  - e49
DO  - 10.1016/j.atherosclerosis.2020.10.150
ER  - 

@conference{
author = "Sopić, Miron and Šupljeglav, Branislava and Milojević, Ana and Mihajlović, Marija and Ninić, Ana and Munjas, Jelena and Gardijan, Vera and Radosavljević, Vojislav and Memon, Lidija and Zdravković, Marija and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background and Aims: Obstructive sleep apnea (OSA) is associated with increased risk of cardiovascular disease. Resistin is recognized as a potent proinflammatory cytokine that is able to influence atherosclerotic plaque progression through several mechanisms. ...",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Increased gene expression of resistin and CD36 in peripheral blood mononuclear cells is associated with hypercholesterolemia in patients with obstructive sleep apnea",
volume = "315",
pages = "e48-e49",
doi = "10.1016/j.atherosclerosis.2020.10.150"
}

Sopić, M., Šupljeglav, B., Milojević, A., Mihajlović, M., Ninić, A., Munjas, J., Gardijan, V., Radosavljević, V., Memon, L., Zdravković, M.,& Spasojević-Kalimanovska, V.. (2020). Increased gene expression of resistin and CD36 in peripheral blood mononuclear cells is associated with hypercholesterolemia in patients with obstructive sleep apnea. in Atherosclerosis
Elsevier., 315, e48-e49.
https://doi.org/10.1016/j.atherosclerosis.2020.10.150

Sopić M, Šupljeglav B, Milojević A, Mihajlović M, Ninić A, Munjas J, Gardijan V, Radosavljević V, Memon L, Zdravković M, Spasojević-Kalimanovska V. Increased gene expression of resistin and CD36 in peripheral blood mononuclear cells is associated with hypercholesterolemia in patients with obstructive sleep apnea. in Atherosclerosis. 2020;315:e48-e49.
doi:10.1016/j.atherosclerosis.2020.10.150 .

Sopić, Miron, Šupljeglav, Branislava, Milojević, Ana, Mihajlović, Marija, Ninić, Ana, Munjas, Jelena, Gardijan, Vera, Radosavljević, Vojislav, Memon, Lidija, Zdravković, Marija, Spasojević-Kalimanovska, Vesna, "Increased gene expression of resistin and CD36 in peripheral blood mononuclear cells is associated with hypercholesterolemia in patients with obstructive sleep apnea" in Atherosclerosis, 315 (2020):e48-e49,
https://doi.org/10.1016/j.atherosclerosis.2020.10.150 . .

TY  - JOUR
AU  - Vuković Dejanović, Vesna
AU  - Kotur-Stevuljević, Jelena
AU  - Vukašinović, Aleksandra
AU  - Miljković, Milica
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Hinić, Saša
AU  - Cerović, Milivoje
AU  - Stefanović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Memon, Lidija
AU  - Nešković, Aleksandar
AU  - Bogavac-Stanojević, Nataša
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3612
AB  - We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.
PB  - SAGE journals
T2  - Angiology
T1  - Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?
VL  - 71
IS  - 8
SP  - 713
EP  - 720
DO  - 10.1177/0003319720921724
ER  - 

@article{
author = "Vuković Dejanović, Vesna and Kotur-Stevuljević, Jelena and Vukašinović, Aleksandra and Miljković, Milica and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Spasojević-Kalimanovska, Vesna and Memon, Lidija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša",
year = "2020",
abstract = "We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.",
publisher = "SAGE journals",
journal = "Angiology",
title = "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?",
volume = "71",
number = "8",
pages = "713-720",
doi = "10.1177/0003319720921724"
}

Vuković Dejanović, V., Kotur-Stevuljević, J., Vukašinović, A., Miljković, M., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Spasojević-Kalimanovska, V., Memon, L., Nešković, A.,& Bogavac-Stanojević, N.. (2020). Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology
SAGE journals., 71(8), 713-720.
https://doi.org/10.1177/0003319720921724

Vuković Dejanović V, Kotur-Stevuljević J, Vukašinović A, Miljković M, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Spasojević-Kalimanovska V, Memon L, Nešković A, Bogavac-Stanojević N. Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology. 2020;71(8):713-720.
doi:10.1177/0003319720921724 .

Vuković Dejanović, Vesna, Kotur-Stevuljević, Jelena, Vukašinović, Aleksandra, Miljković, Milica, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Spasojević-Kalimanovska, Vesna, Memon, Lidija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?" in Angiology, 71, no. 8 (2020):713-720,
https://doi.org/10.1177/0003319720921724 . .

TY  - JOUR
AU  - Ninić, Ana
AU  - Zdravković, Marija
AU  - Radosavljević, Vojislav
AU  - Gardijan, Vera
AU  - Memon, Lidija
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3769
AB  - Obstructive  sleep  apnea  (OSA)  as  a  worldwide  prevalent  condition  carries  risk  for cardiovascular  and  metabolic  diseases,  ultimately  increasingoverall  mortality  rates.  Non-alcoholic fatty liver disease  (NAFLD) can be considered as the primary metabolic  disease,  but also as a coexisting OSA comorbidity. Although prevalence of NAFLD covers quarter of world population, it increases with OSA presence. It can be speculated that chronic intermittent hypoxia (CIH)  and  sympathetic  nervous  system  overactivity  are  involved  in  NAFLD  pathogenesis  and progression   from   simple   steatosis throughsteatohepatitis   to   fibrosis.   CIH   provides   the environment  for  liveroxidative  stress,  inflammation  and  increases  the  expression  of  genes involved in cholesterol and fatty acids synthesis. Catecholamines increase β-oxidation in liver and release free fatty acids from adipose tissue in plasma which inhibit insulin effects. Obesity and insulin resistance as key playersin NAFLD development and advancement, deepen vicious circle of oxidative stress, inflammation and dyslipidemia. If not treated, OSA in NAFLD patients has been  associated  with inflammation, hepatocytes’ necrosis,  and  fibrosis.  Continuous  positive airway pressure (CPAP) represents gold standard for OSA therapy, allowing the unimpeded air passage through upper parts of respiratory system. However, it has been demonstrated that CPAP therapyhave beneficial effects on cardiometabolic outcomes and slowliver degeneration
AB  - Opstruktivna apneja u snu (OSA) kao oboljenje prevalentno u svetu nosi rizik za nastanak kardiovaskularnih i metaboličkih bolesti, povećavajući ukupnu smrtnost. Bolest nealkoholne masne jetre (eng. non-alcoholic fatty liver disease -NAFLD) se može smatrati primarnom metaboličkom bolešću, ali kao i komorbiditet OSA. Iako prevalenca NAFLD obuhvata četvrtinu svetske populacije, ona se povećava sa prisustvom OSA. Pretpostavlja se da su hronična intermitentna hipoksija (eng. chronic intermittent hypoxia -CIH) i prekomerna aktivnost simpatičkog nervnog sistema uključeni u patogenezu NAFLD i progresiju od steatoze preko steatohepatitisa do fibroze. U jetri CIH stvara uslove za oksidativni stres, inflamaciju i povećava ekspresiju gena koji učestvuju u sintezi holesterola i masnih kiselina. Kateholamini stimulišu b-oksidaciju masnih kiselina u jetri i oslobađaju slobodne masne kiseline iz masnog tkiva u plazmu, koje inhibiraju dejstva insulina. Gojaznost i insulinska rezistencija kao ključni faktori u razvoju i napredovanju NAFLD produbljuju začarani krug oksidativnog stresa, inflamacije i dislipidemije. Ako se ne leči, OSA kod pacijenata sa NAFLD je povezana sa inflamacijom, nekrozom hepatocita i fibrozom. Kontinuirani pozitivni pritisak vazduha (eng. continuous positive airway pressure -CPAP) predstavlja zlatni standard u terapiji OSA, koji omogućava neometani prolaz vazduha kroz gornje delove respiratornog sistema. Međutim, CPAP terapija je pokazala da ima povoljne efekte na kardiometaboličke ishode i da usporava degeneraciju jetre.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Non-alcoholic fatty liver disease as metabolic consequence of obstructive sleep apnea
T1  - Bolest nealkoholne masne jetre kao metabolička posledica opstruktivne apneje u snu
VL  - 70
IS  - 6
SP  - 319
EP  - 331
DO  - 10.5937/arhfarm70-27586
ER  - 

@article{
author = "Ninić, Ana and Zdravković, Marija and Radosavljević, Vojislav and Gardijan, Vera and Memon, Lidija and Vekić, Jelena and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Obstructive  sleep  apnea  (OSA)  as  a  worldwide  prevalent  condition  carries  risk  for cardiovascular  and  metabolic  diseases,  ultimately  increasingoverall  mortality  rates.  Non-alcoholic fatty liver disease  (NAFLD) can be considered as the primary metabolic  disease,  but also as a coexisting OSA comorbidity. Although prevalence of NAFLD covers quarter of world population, it increases with OSA presence. It can be speculated that chronic intermittent hypoxia (CIH)  and  sympathetic  nervous  system  overactivity  are  involved  in  NAFLD  pathogenesis  and progression   from   simple   steatosis throughsteatohepatitis   to   fibrosis.   CIH   provides   the environment  for  liveroxidative  stress,  inflammation  and  increases  the  expression  of  genes involved in cholesterol and fatty acids synthesis. Catecholamines increase β-oxidation in liver and release free fatty acids from adipose tissue in plasma which inhibit insulin effects. Obesity and insulin resistance as key playersin NAFLD development and advancement, deepen vicious circle of oxidative stress, inflammation and dyslipidemia. If not treated, OSA in NAFLD patients has been  associated  with inflammation, hepatocytes’ necrosis,  and  fibrosis.  Continuous  positive airway pressure (CPAP) represents gold standard for OSA therapy, allowing the unimpeded air passage through upper parts of respiratory system. However, it has been demonstrated that CPAP therapyhave beneficial effects on cardiometabolic outcomes and slowliver degeneration, Opstruktivna apneja u snu (OSA) kao oboljenje prevalentno u svetu nosi rizik za nastanak kardiovaskularnih i metaboličkih bolesti, povećavajući ukupnu smrtnost. Bolest nealkoholne masne jetre (eng. non-alcoholic fatty liver disease -NAFLD) se može smatrati primarnom metaboličkom bolešću, ali kao i komorbiditet OSA. Iako prevalenca NAFLD obuhvata četvrtinu svetske populacije, ona se povećava sa prisustvom OSA. Pretpostavlja se da su hronična intermitentna hipoksija (eng. chronic intermittent hypoxia -CIH) i prekomerna aktivnost simpatičkog nervnog sistema uključeni u patogenezu NAFLD i progresiju od steatoze preko steatohepatitisa do fibroze. U jetri CIH stvara uslove za oksidativni stres, inflamaciju i povećava ekspresiju gena koji učestvuju u sintezi holesterola i masnih kiselina. Kateholamini stimulišu b-oksidaciju masnih kiselina u jetri i oslobađaju slobodne masne kiseline iz masnog tkiva u plazmu, koje inhibiraju dejstva insulina. Gojaznost i insulinska rezistencija kao ključni faktori u razvoju i napredovanju NAFLD produbljuju začarani krug oksidativnog stresa, inflamacije i dislipidemije. Ako se ne leči, OSA kod pacijenata sa NAFLD je povezana sa inflamacijom, nekrozom hepatocita i fibrozom. Kontinuirani pozitivni pritisak vazduha (eng. continuous positive airway pressure -CPAP) predstavlja zlatni standard u terapiji OSA, koji omogućava neometani prolaz vazduha kroz gornje delove respiratornog sistema. Međutim, CPAP terapija je pokazala da ima povoljne efekte na kardiometaboličke ishode i da usporava degeneraciju jetre.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Non-alcoholic fatty liver disease as metabolic consequence of obstructive sleep apnea, Bolest nealkoholne masne jetre kao metabolička posledica opstruktivne apneje u snu",
volume = "70",
number = "6",
pages = "319-331",
doi = "10.5937/arhfarm70-27586"
}

Ninić, A., Zdravković, M., Radosavljević, V., Gardijan, V., Memon, L., Vekić, J.,& Spasojević-Kalimanovska, V.. (2020). Non-alcoholic fatty liver disease as metabolic consequence of obstructive sleep apnea. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 70(6), 319-331.
https://doi.org/10.5937/arhfarm70-27586

Ninić A, Zdravković M, Radosavljević V, Gardijan V, Memon L, Vekić J, Spasojević-Kalimanovska V. Non-alcoholic fatty liver disease as metabolic consequence of obstructive sleep apnea. in Arhiv za farmaciju. 2020;70(6):319-331.
doi:10.5937/arhfarm70-27586 .

Ninić, Ana, Zdravković, Marija, Radosavljević, Vojislav, Gardijan, Vera, Memon, Lidija, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, "Non-alcoholic fatty liver disease as metabolic consequence of obstructive sleep apnea" in Arhiv za farmaciju, 70, no. 6 (2020):319-331,
https://doi.org/10.5937/arhfarm70-27586 . .